Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: Enrichment of normal progenitors in counter-flow centrifugal elutriation (CCE) fractions of fresh chronic myeloid leukemia leukapheresis products.
Autorzy: Dłubek Dorota, Dybko Jarosław, Wysoczańska Barbara, Laba Anna, Klimczak Aleksandra, Kryczek Ilona, Konopka Lech, Lange Andrzej
Źródło: Eur. J. Haematol. 2002: 68 (5) s.281-288, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 304,912

Hasła klasyfikacyjne GBL:
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Objectives: The aim of this study was to assess the suitability of a technique based on counter-flow centrifugal elutriation (CCE), which should allow one to enrich chronic myeloid leukemia (CML) patients' unstimalated native leukapheresis product (nLP) in CD34+ HLADR cells and BCR-ABL negative cells. Methods: Six newly diagnosed CML patients were subjected to leukapheresis, and the products were subfractionated with the use of CCE. nLP and all fractions were studied for the presence of CD34+ cells and a proportion of BCR-ABL fluorescence in situ hybridization (FISH)+ cells. Results: CCE fractions with a high flow rate contained the highest proportion of CD34+ cells [mean (SEM) 6.89 p.c. (3.88)]. However, CD34+ cells present in low-rate CCE fractions showed a higher proportion of HLADR- [49.6 p.c. (13.5 in 70 mL min -1) and 21.5 p.c. (11.6 in 110 mL min-1)] than those in 170 mL min-1 [3.2 p.c.(2.5)] and 'rotor off'[3.4 p.c. (1.9)]. This was associated with lower proportions of BCR-ABL FISH+ [8.1 p.c. (4.8) and 1.9 (1.7)] and smaller BCR-ABL to ABL transcript ratios [0.58 (17) and 0.26 (0.08) in 70 and 110 mL min-1] fractions as compared to 140 and 170 mL min-1 fractions [21.6 p.c. (5.2) and 31.6 p.c. (15.3) for BCR-ABL FISH+ cells and 0.75 (0.16) and 0.90 (0.24) for BCR-ABL/ABL]. Fractions with the lowest proportions of BCR-ABL-positive cells and the lowest BCR-ABL/ABL transcript ratios (110 mL min-1) contained from 1.3 x 10**6 to 82.7 x 10**6 (median: 3.97 x 10**6) CD34+ cells. Conclusions: In the present study we have shown that CCE may be used effectively to obtain nLP fractions enriched in normal hematopoietic progrenitors.

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