Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: Melanin potentiates gentamicin-induced inhibition of collagen biosynthesis in human skin fibroblasts.
Autorzy: Wrześniok Dorota, Buszman Ewa, Karna Ewa, Nawrat Piotr, Pałka Jerzy
Źródło: Eur. J. Pharmacol. 2002: 446 (1/3) s.7-13, il., bibliogr. s. 12-13
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:
  • farmacja
  • toksykologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: One of the recognized side effects of gentamicin is ototoxicity. The mechanism underlying the organ specificity of this side effect of gentamicin has not been fully established. In view of the fact that a number of pharmacologic agents are known to from complexes with melanin and melanin is an abundant constituent of the inner ear tissues, we determined whether gentamicin interacts with melanin and how this process affects the biosynthesis of collagen in cultured human skin fibroblasts. Our results indicate that gentamicin forms comples with melanin. The amount of gentamicin bound to melanin increases with increasing of initial drug concentration. The Scatchard plot analysis of drug binding to melanin showed that at least two classes of independent binding sites are implicated in gentamicin-melanin complex formation: one class with an association constant K1 ~ 4 x 10**3 M**-1, and the second class with an association constant K2 ~ 3 x 10**2 M**-1. The number of total binding sites (n1 + n2) was calculated as about 1.36 ćmol gentamicin per 1 mg melanin. We have suggested that prolidase, an enzyme involved in collagen metabolism, may be one of the targets for gentamicin-induced inhibition of collagen biosynthesis. We found that gentamycin-induced inhibition of prolidase activity (IC50 ~ 100 ćM) and collagen biosynthesis (IC50 ~ 100 ćM). At this concentration of gentamicin, DNA biosynthesis in human skin fibroblasts was inhibited only by about 30 p.c. Melanin at 100 ćg/ml produced about 25 p.c. inhibition of DNA synthesis and about 30 p.c. inhibition of prolidase activity, ...


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    Tytuł oryginału: Wpływ jonów Cu2+ i Zn2+ na wiązanie prokainy i tetrakainy do melaniny.
    Tytuł angielski: The effect of Cu2+ and Zn2+ on procaine and tetracaine binding to melanin.
    Autorzy: Buszman Ewa, Betlej Bożena, Wrześniok Dorota
    Źródło: Ann. Acad. Med. Siles. 2002: 50/51 s.13-23, il., tab., bibliogr. 21 poz., sum.
    Sygnatura GBL: 313,709

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna

    Wskaźnik treści:
  • in vitro

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