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Zapytanie: WOŁUŃ-CHOLEWA
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Tytuł oryginału: The influence of photodynamic reaction on R2C cells.
Autorzy: Wołuń-Cholewa M[aria], Warchoł J[erzy] B.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.109-110, il., bibliogr. 10 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • in vitro

    Streszczenie angielskie: Photodynamic therapy (PDT) represents a theapeutic approach in which photosensitised neoplastic cells undergo destruction under effect of light. In this study we have attempted to define effects of photochemotherapy R2C cells, sensitised with protoporphyrin IX (PpIX) and to find out whethe inhibition of gene expression by cycloheximide affects development of lesions in the cells. The photosensitised necrotic lesions was followed in the cells, using the fluorescent staining with propidium iodide and Hoechst 33342. The expresiments demonstrated that PpIX and light, acting in parallel, induce development of apoptotic and necrotic lesions in R2C cells. Intensity of the lesions correlated with concentration of the applied photosensitiser and with duration of light exposure. Using cycloheximide, we also inhibited protein expression in cells photosensitised with protoporphyrin before they were exposed to light. In the latter case, development of apoptosis was clearly intensified which might be explained by inhibition of antiapoptotic protein synthesis in the cells.

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