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Evaluation of the usefulness of the ELISA method for detection of enterovirus antibodies in serum samples of patients with myocarditis.
Med. Sci. Monitor 2002: 8 (1) s.MT10-MT14, il., bibliogr. 26 poz.
Hasła klasyfikacyjne GBL:
Background: Enteroviruses are among the etiological factors in myocarditis, In the majority of cases it is impossible to obtain a direct confirmation of the presence of the virus, and typologically specific methods are of limited applicability, due both to the number of enteroviruses that can cause myocarditits, and to the serological cross reactions among them. The goal of our research was to determine the usability of the ELISA test and the complement fixation reaction (CFR) in diagnostic testing of the serum of patients with myocarditis. Material/Methods: The research material consisted of 1197 serum samples collected from patients with myocarditis. In all these samples we measured the contentration of IgG adn IgM enterovirus antibodies using the ELISA method, and teh height of the titer using the CFR method. Results: The presence of IgM antibodies is a confirmation of current infection. A postitive result was obtained for 61 p.c. of the patients. On the other hand, if one accepts as confirmation of infection a titer significantly different from the level observed in thy healthy population, namely ň 16 for CFR and ň 11 for ELISA-IgG (cf. positive results of 33 p.c. for CFR and 18.8 p.c. for ELISA IgG in our patients), the results may be disproportional. If one assumes as a test criterion a significant increase in titer, the presence of infection was confirmed in 28.5 p.c. of cases for CFR and 62 p.c. for ELISA-IgG. Conclusions: An analysis of the concentration of IgG antibodies constitutes a clear confirmation of infection., if the criterion of evaluation is based on a measure in a single serum sample. In this case it is necessary to test for a significant increase in the titer. The use of the average titer for the population at large to evaluate results leads to both false positives and false negatives. Testing for IgM antibodies in a single serum sample is a faster method which confirms the current presence of infection. Our results indicate that the ELISA test can be used to diagnose myocarditis, since
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