Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: Detecting the replication of the hepatitis B virus using the ImmunoMax technique following treatment with Interferon-alpha in children with chronic hepatitis.
Autorzy: Kasprzak Aldona, Wysocki Jacek, Zabel Maciej, Surdyk-Zasada Joanna
Źródło: Med. Sci. Monitor 2002: 8 (1) s.PR1-PR7, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • pediatria
  • immunologia
  • mikrobiologia
  • gastroenterologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Children with HBV in Poland are treated with preparations of interferon-alpha (IFN-ŕ). The continuing laack of complete response to this type of anti-viral therapy remains to be explained. The application of cell biology techniques to identify the viral components in situmakes it possible to clarify the association between the distribution of the virus and morphological injury to the liver, the immune response of the host, and clinical symptoms in the natural course of infection. Our study was intendent to evaluate HBV expression in liver biopsies taken an average of two years after completion of IFN-ŕ therapy in 10 children with serological markers of persistent HBV infection. Materil/Methods: For the immunocytochemical detecion of HBcAg and for the hybridocytochemical detection of HBV-DNA, the avidin-biotin-peroxidase (ABC) technique was employed, as well as classical in situ hybridization, both additionally amplified using the ImmunoMax technique. HBcAg and HBV-DNA levels were estimated using a semiquantitative technique. Resuslts: Our study demonstrated persistent active replication of HBV in the liver in all examined children. A mixed pattern of HBcAg localization prevailed (noted in cell nuclei, cytoplasm and cell membranes) with a somewhat lower proportion of involved cells and a more evident membrane localization of HBcAg, as compared to results obtained before treatment. HBVDNA was observed in the cytoplasm of a fraction of hepatocytes similar to that noted before therapy. Conclusions: The ImmunoMax technique was found to be highly suitable for in situ monitoring of HBV replication after termination of IFN-ŕ treatment. Children with focal distribution of HBcAg andHBV-DNA have the potential for earlier eradication of the virus from their livers.


    Tytuł oryginału: Immunolocalization of PTHrP in prepubertal and pubertal testis of European bison.
    Autorzy: Czykier Elżbieta, Zabel Maciej, Surdyk-Zasada Joanna
    Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.373-375, il., bibliogr. 23 poz.
    Sygnatura GBL: 304,846

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • urologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: The present study deals with immunohistochemical localization of PTHrP in prepubertal and pubertal testis of European bison. PTHrP immunoreactivity was observed in germinal cells in the testis of both prepubertal and pubaertal animals. In calves, PTHrP was found in germinal cells, in seminiferous tubules lacking the lumen. The reaction was strong and regularly distributed within the cytoplasm. In adult animals, the reaction showed differentiation in spermatogenic cells. Some cells were strongly and diffusely stained, others exhibited weaker reaction of granular pattern. Sertoli cells and Leydig cells were PTHrP-negative in calves and adult animals.

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