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Tytuł oryginału: The role of G protein B subunits in the release of ATP from human erythrocytes.
Autorzy: Sprague R. S., Bowles E. A., Olearczyk J. J., Stephenson A. H., Lonigro A. J.
Źródło: J. Physiol. Pharmacol. 2002: 53 (4 p. 1) s.667-674, il., bibliogr. 20 poz.
Sygnatura GBL: 302,092

Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Previously, we demonstarted that adenosine triphosphate (ATP) is released from human erythrocytes in response to mechanical deformation and that this release requires activation of a signal-transduction pathway involving adenylyl cyclase and the heterotrimeric G protein, Gs. Here we investigate the role of heterotrimetric G proteins of the Gi subtype in the release of ATP from human erythrocytes. In addition, we determined the profile of heterotrimeric G protein á submits present in these erythrocyte membranes. The activity of GI was stimulated by incubation of erythriocytes (20 p.c. hematocrit) with mastopain (10 ćM). ATP release was measured using the luciferin/luciferase assay. Heterotrimeric G protein á subunits present in erythrocyte membranes were resolved using gel electrophoresis and subunit specific antibodies. Incubation of human erythrocytes with mastoparan (an activator ofGi/o) resulted in a 4.1 ń 0.6-fold increase in ATP present in the medium (P 0.01). Human erythrocyte membranes stain positively for á subunit types 1,2,3, and 4, all of which been reported to activate of some isoforms of adenylyl cyclase. Activation of theheterotrimeric G protein, Gi, results in ATP release from erythrocytes. This effect is may be related to the activity of á subunits associated with this G protein in the human erythrocyte.

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