Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SKŁADANOWSKI
Liczba odnalezionych rekordów: 3



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Tytuł oryginału: Intercalation of imidazoacridinones to DNA and its relevance to cytotoxic and antitumor activity.
Autorzy: Dzięgielewski Jarosław, Ślusarski Bartłomiej, Konitz Antoni, Składanowski Andrzej, Konopa Jerzy
Źródło: Biochem. Pharmacol. 2002: 63 (9) s.1653-1662, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Imidazoacridinones (IA) are a class of antitumor agents which includes C-1311, an interesting drug in clinical trials. This study investigated the mechanism of IA binding to DNA for a series of 13 analogs that differ in their cytotoxic potency. Using C-1311 as a model compound, crystallographic, spectroscopic and biochemical techniques were employed to characterize drug-DNA interactions. X-ray crystallographic analysis revealed a planar structure of imidazoacridinone core that is capable of intercalative DNA bibding. Accordingly, C-1311 binding to DNA followede "classical" pattern observed for intercalation, as proved by tha DNA topoisomerase I - unwinding experiments, with relatively weak binding affinity (Ki = 1.2 x 10**5 M-1), and the binding site size of 2.4 bp. Other IA also bound to DNA with the binding affinity in the range of 10**5 M-1 and binding site size of 2-3 bp, suggesting a prevalence of the intercalative mechanism, similar to C-1311. Considerable DNA binding affinity was displayed by all highly cytotoxic derivatives. However, none of the analyzed drug-DNA binding parameters was significantly correlated with IA biological activities such as cell growth. DNA and RNA synthesis inhibition, or tumor growth inhibition, which suggests that the IA ability to non-covalently bind to DNA is not crucial for their biological activity. These results show that the ability to intercalate into DNA is a prominent attribute of IA, although factors other intercalative binding seem to be required for the biological activities of IA drugs.

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    Tytuł oryginału: Influence of G2 arrest on the cytotoxicity of DNA topoismerase inhibitors toward human carcinoma cells with different p53 status.
    Autorzy: Bożko Przemysław, Larsen Annette K., Raymond Eric, Składanowski Andrzej
    Źródło: Acta Bioch. Pol. 2002: 49 (1) s.109-119, il., bibliogr. 29 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
    Sygnatura GBL: 303,116

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • genetyka
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-incubation in drug-free medium. RKO cells expressing wild-type, functional p53 arrested the cell cycle progression in both the G1 an G2 phases of the cell cycle whereas teh RKO/E6cells, which lack functional p53, only arrested in the G2 phase. Growth-arrested cells did not resuem proliferetion even after prolonged incubation in drug-free medium (up to 96 h). To evaluate the importance of the cell cycle arrest on cellular survival, a non-toxic dose of UCN-01 (100 nM) was added to the growth-arrested cells. The addition of UCN-01 was accompanied by mitotic entry as revealed by the appearance of condensed chromatin and the MPM-2 phosphoepitope, which is charactieristic for mitotic cells, G2 exit and mitotic transit was accompanied by a rapid activation of caspase-4 and apoptotic cell death. The influence of UCN-01 on the long-term cytotoxic effects of the two drugs was also determined. Unexpectedly, abrogation of the G2 arrest had no influence on the overall cytotoxicity of either drug. In contrast, addition of UCN-01 to cisplatin-treated RKO and RKO/E6 cells greatly increased the cytotoxic effects of the alkylating agent. These results strongly suggest that even prolonged cell cycle arrest in the G2 phase of the cell cycle is not necessarily coupled to efficient DNA repair and enhanced cellular survival as generally believed.

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    Tytuł oryginału: Stanisław Żołnierowicz (1955-2001) - dr hab. nauk medycznych, profesor nadzwyczajny UG, nauczyciel akademicki na Międzyuczelnianym Wydziale Biotechnologii UG-AMG.
    Autorzy: Bigda Jacek, Składanowski Andrzej C., Tymińska Elżbieta
    Źródło: Ann. Acad. Med. Gedan. 2002: 32 s.511-515, il.
    Sygnatura GBL: 702,634

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca biograficzna
  • bibliografia

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • historia nowożytna
  • historia XX wieku

    Temat osobowy:
  • Żołnierowicz
  • Stanisław 1955-2001

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