Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SINGH
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1/5

Tytuł oryginału: Functional reconstitution of Ral-binding GTPase activating protein, RLIP76, in proteoliposomes catalyzing ATP-dependent transport of glutathione conjugate of 4-hydroxynonenal.
Autorzy: Sharma Rajendra, Sharma Abha, Yang Yusong, Awasthi Sanjay, Singhal Sharad S., Zimniak Piotr, Awasthi Yogesh C.
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.693-701, il., bibliogr. s. 700-701
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Streszczenie angielskie: Earlier studies from our laboratories have shown that RLIP76, a previously described Ral-binding GTpase activating protein (Jullien-Flores et al., 1995, J. Biol. Chem. 270: 22473), is identical with the xenobiotic transporter DNP-SG ATPase, adn can catalyze ATP-dependent transport of glutathione-conjugates as well as doxorubin (Awasthi et al., 2000, Biochemistry, 39: 9327). We have now reconstituted purified bacterially expressed RLIP76 in proteoliposomes, and have studied ATP-dependent uptake of the glutathione conjugate of 4-hydroxynonenal (GS-HNE) by these vesicles. Results of these studies show that RLIP76 reconstituted in proteoliposomes catalyzes ATP-dependent transport of GS-HNE against a concentration gradient. The transport of GS-HNE is saturable with respect to ATP as well as GS-HNE with Km values of 1.4 mM and 2.5 ćM, respectively. These studies demonstrate that RLIP76 mediates active transport of GS-HNE, adn are consistent with our previous work showing that RLIP76-mediated efflux of GS-HNE regulates the intracellular concentration of 4-HNE and thereby affects 4-HNE mediated signaling.

    2/5

    Tytuł oryginału: Antigenic and allergenic properties of Amaranthus spinosus Pollen - a commonly growing weed in India.
    Autorzy: Singh Anand B., Dahiya Pushpa
    Źródło: Ann. Agric. Environ. Med. 2002: 9 (2) s.147-151, il., tab., bibliogr. 22 poz.
    Sygnatura GBL: 313,158

    Hasła klasyfikacyjne GBL:
  • immunologia

    Typ dokumentu:
  • praca kliniczna
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Amaranthus spinosus (Fam. Amaranthaceae) is an important aeroallergen in India and grows commonly in different parts of the country. In spite of its clinical significance in Type I hypersensitivity disorders, the antigenic and the allergenic properties of the epollen have not been systematically resolved. We investigated antigenic and allergenic properties of 5 pollen samples of Amaranthus spinosus collected from the Delhi area of fortinghtly intervals. The protein content did not exhibit statistically significant variability. However, samples collected during the peak flowering season showed higher protein content. Biochemical characterization of samples showed multiple protein fractions bay IEF and SDS-PAGE analysis. Samples collecated during peak sesason showed a slightly higher number of bands (22) in the mw range of 14-70 kD. Seven protein fractions of 70, 66, 60, 50, 40, 30 and 14 kD were observed to have IgE binding capabilities and 9 were treated as allergenic. The observations will be helpful in standardizing pollen antigens for diagnosis and immunotherapy in India.

    3/5

    Tytuł oryginału: Transport functions and physiological significance of 76 kDa Ral-binding GTPase activating protein (RLIP76).
    Autorzy: Awasthi Sanjay, Sharma Rajendra, Yang Yusong, Singhal Sharad S., Pikula Sławomir, Bandorowicz-Pikula Joanna, Singh Shivendra V., Zimniak Piotr, Awasthi Yogesh C.
    Źródło: Acta Bioch. Pol. 2002: 49 (4) s.855-867, il., tab., bibliogr. s. 863-867
    Sygnatura GBL: 303,116

    Typ dokumentu:
  • tytuł obcojęzyczny

    Streszczenie angielskie: We have recently demonstrated that a previously known Ral-binding GTPase activating protein, RLIP76, can also catalyze ATP-dependent transport of various structurally unrelated xeno- and endobiotics irrespective of their net charge (Awasthi et al., 2000, Biochemistry, 39: 9327). RLIP76 is a non-ATP binding cassette (ABC) protien but it has two ATP-binding sites and shows basal ATPase activity which is stimulated in the presence of its transport substrates (allocrites) such as doxorubicin (DOX) and S-(2,4-dinitrophenyl) glutathione (DNP-SG). Proteoliposomes reconstituted with purified RLIP76 catalyze ATP-dependent, saturable transport of DOX, as well as of glutathione-conjugates including leukotrienes (LTC4) and the GSH-conjugate of 4-hydroxynonenal (GS-HNE). In erythrocytes the majority of transport activity for DOX, GS-HNE, and LTCA is a ccounted for by RLIP76. Cells exposed to mild oxidative stress show a rapid and transient induction of RLIP76 resulting in an increased efflux of GS-HNE and acquire resistance to oxidative stress mediated toxicity and apoptosis. Cells transfected with RLIP76 acquire resistance to DOX through increased efflux of the drug suggesting its possible role in the mechanisms of drug-resistance. In this article, we discuss the significance of transport functions of RLIP76 highlighting its role in the defense mechanisms against oxidative injury, and modulation of signaling mechanisms.

    4/5

    Tytuł oryginału: Potential of CT-scan based tumor volume as a response indicator in chemotherapy of advanced epithelial ovarian cancer.
    Autorzy: Kumar Pratik, Rehani Madan Mohan, Kumar Lalit, Sharma Raju, Bhatla Neerja, Singh Rajvir, Sundaram K. Ramaiyer
    Źródło: Med. Sci. Monitor 2002: 8 (10) s.CR667-CR674, il., tab., bibliogr. 27 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • onkologia
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: Background: Response prediction in patients undergoing chamotherapy for ovarian cancer is an important issue, since the cure rate is only about 15 - 20 p.c. We attempted to develop a semi-empirical model to predict response in individual cases after the first cycle of chemotherapy. Material/Methods: This prospective study included 51 cases of advanced ovarian cancer. A method was standardized to estimate ovarian tumor volume accurately from CT scan films. This permits the inclusion of patients who have undergone CT scan elsewhere. Patients underwent 4 - 6 cycles of chemotherapy and tumor volume was estimated after each cycle. This yielded a tumor regression curve for each patient. Results: Percent reduction in tumor volume after the first chemo-cycle was a significant prognostic factor in multivariate analysis. Depending upon the rate of regression patients could be classified into Fast Regressing FR (n = 29) and Moderately Regressing MR (n = 16), whereas 6 patients showed Progressive Disease (PD) despite ongoing chemotherapy. The median survivals for the FR, MR and PD groups were 29.3, 18.9 adn 8.5 months respectively. We found that 'percent reduction in volume after firs chemo-cycle' could categorize a patient as FR/MR/PD correctly in 94.1 p.c. of cases. This parameter could also detect 5 out of 6 inherently resistand PD cases, who would otherwise undergo further chemotherapy, since early detection of resistance by clinical monitoring is quite difficult.

    5/5

    Tytuł oryginału: Alcohol induced ketoacidosis, severe hypoglycemia and irreversible encephalopathy.
    Autorzy: Jain Hitender, Beriwal Sushil, Singh Shailender
    Źródło: Med. Sci. Monitor 2002: 8 (11) s.CR77-CR79, il., bibliogr. 4 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • psychiatria i psychologia
  • neurologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kazuistyczna

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Background: Severe hypoglycemia leading to permanent brain damage is rare in non-daibetic population. We present one such case where chronic alcoholism combined with prologned fasting lead to such a state and patient ended in a persistent vegetative state. Case report: A 50 year old Asian woman with past history of chronic alcoholism and hepatitis C was found unresponsive in her house after drinking alcohol consecutively for 2 days. Finger-stick glucose done by paramedics was 20 mg/dl. She was given 50 ml of 50 p.c. dextrose without any neurological response. On arrival in the emergency department patient was found to be comatose with only withdrawal response to deep painful stimuli and a negative babinski's sign. Computered tomogram of the head revealed mild cerebral atrophy. After an extensive work up a diagnosis of alcoholic ketoacidosis with hypoglycemia related encephalopathy was made. Conclusion: Alcohol induced ketoacidosis is usually associated with normal blood glucose. The probable etiology of low blood sugar in our patient was alcohol-induced inhibition of gluconeogenesis along with starvation. The prolonged hypoglycemia caused cortical damage simulating ischemic brain damage. Ten months in to follow-up patient is still in persistent vegetative state with no noticeable neurological recovery.

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