Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SCHULTHEISS
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Tytuł oryginału: Digital image analysis system for the quantification of infiltrates and cell adhesion molecules in inflammatory cardiomyopathy.
Autorzy: Noutsias Michel, Pauschinger Matthias, Ostermann Karsten, Escher Felicitas, Blohm Jan-Henrik, Schultheiss Heinz-Peter, Khl Uwe
Źródło: Med. Sci. Monitor 2002: 8 (5) s.MT59-MT71, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We attempted to develop a digital image analysis (DIA) system for endomyocardial biopsies (EMBs) to reliably quantify a) biopsy quality, b) immunohistochemically-marked infiltrates, and c) cell adhesion molecules (CAMs) in relation to net heart area (HA) for the semi-automated diagnosis of inflammatory cardiomyopathy (InfCM). 140 EMBs from dilated cardiomyopathy (DCM) patients and 14 autopsy heart samples (controls) were immunostained for T-lymphocytes (CD2, CD3, CD4, CD8), á2-integrin + infiltrates (CD18, LFA-1, Mac-1) and CAMs (immunoglobulin superfamily: ICAM-1, HLA class I, HLA DR, VCAM-1, CD58; selectins: CD62E and CD62P; and the á1-integrin chain CD29). EMB quality was assessed visually on a three-point scale. Infiltrates were quantified visually (per hpf) and by DIA (per mm2 HA). CAM expression was evaluated semiquantitatively and bay DIA (area fraction [AF]: stained area relative to HA). DIA-evaluated HA correlated significantly with the visual assessment of EMB quality. The visual evaluation of both infiltrates and CAMs correlated significantly with the respective DIA-based quantification. DIA-quantified CAM-AF and infiltrates were discriminated bay the CAM clssification (CAMs+: n = 87; 62 p.c.) compared to controls. DIA-quantified cCAM immunoreacativity correlated significantly with the DIA-quantified counter-receptor + infiltrates. DIA evaluation of biopsy quality, infiltrates, and CAMs was devoid of inter- and intraobserver variability. The DIA system presented here enables standardized and observer-independent assessment of EMB quality and intramyocardial...

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    Tytuł oryginału: Phenotypic characterization of infilatrates in dilated cardiomyopathy - diagnostic significance of T-lymphocytes and macrophages in inflammatory cardiomyopathy.
    Autorzy: Noutsias Michel, Pauschinger Matthias, Schultheiss Heinz-Peter, Khl Uwe
    Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR478-CR487, il., tab., bibliogr. 55 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • immunologia
  • kardiologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background: Dilated cardiomyopathy (DCM) is linked to inflammatory cardiomyopathy (InfCM). To gain a profound insight into the underlying mechanisms, we phenotypically characterized pan leukocytes (CD18), naive T-lymphocytes (CD3, CD2, CD4, CD8), activated lympocytes (LFA-1, LFA-3, VLA-4, ICAM-1, CD69, CD45RO), macrophages (Mac-1, 27E10), B-lymphocytes (CD19) and NK-cells (CD57) in DCM and control hearts. Material/Methods: Biopsies from DCM patietns (n = 164, LVEF 40 p.c.) and specimens from non-cardiac death causes (controls; n = 17) were immunostained. Biopsies exceeding 2.0 CD3+ lymphocytes per high power field/hpf and/or 1.5 CD3+ lymphocytes/hpf with numerous foci and HLA class I/DR abundance were evaluated positive for InfCM. Results: InfCM+ biopsies (n = 102; 63 p.c.) demonstrated significantly increased infiltrates with respect to all studied phenotypes except for CD19 and CD57 when compared with both DCM biopsies negative for InfCM (n = 62) and the controls, whereas the latter two groups did not differ (Tukey-Kramer analysis). Virtually all phenotypes correlated with one another in multivariate analysis (except for B-lymphocytes and NK cells). Whereas HLA class I/DR abundance was present in 14 p.c. of the controls and 26 p.c. of the DCM biopsies not yielding InflCM, InfCM+ biopsies demonstrated significantly ( 0.001) higher frequencies of HLA abundance (76 p.c.). Conclusions: The inflammatory process in InfCM comprises T-lymphocytes and macrophages, whereas B-lymphocytes and NK-cells are not significantly increased. InfCM is associated with HLA induction...

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