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Zapytanie: SAWIŃSKI
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Tytuł oryginału: The influence of interleukin-1 and interleukin-6 on expression of proteins involved in steroidogenesis in Leydig cells and on their apoptosis or necrosis in vitro.
Autorzy: Sawiński P[iotr], Łukaszyk A[ndrzej]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.159-160, il., tab., bibliogr. 8 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:
  • immunologia
  • urologia
  • endokrynologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • in vitro

    Streszczenie angielskie: This paper presents a study on the expression of proteins involved in steroidogenesis in rat Leydig cells: StAR protein and aromatase, in relation to the age of animals and to the influence of interleukin-1 and interleukin-6. In addition, their effect on apoptotis and damage of Leydig cells was investigated. Leydig cells were isolated rom rats at certain age intervals and exposed to different concentrations of the above-mentioned cytokines. Next, total RNA was isolated from Leydig cells and RT-PCR with primers specific for rat StAR protein and aromatase was performed. The products were analysed by agarose gel electrophoresis. Leydig cells were also stained with propidium iodide and Hoechst 33342 to estimate the percentage of apoptotic or necrotic or necrotic cells.The results seem to confirm the role of interleukins in the control of expression of enzymes and regulatory factors involved in steroidogenesis in rat Leydig cells, and in the regulation of their apoptosis and necrosis. The effect of the studied interleukins on Leydig cells is exerted during development and after adolescence.

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