Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SARKAR
Liczba odnalezionych rekordów: 2

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Tytuł oryginału: Carboxy terminal domain of the largest subunit of RNA polymerase II of Leishmania donovani has an unusually low number of phophorylation sites.
Autorzy: Dasgupta Arindam, Sharma Shalini, Das Aditi, Sarkar Dwijen, Majumder Hemanta K.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR341-CR350, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: The C-terminal domain (CTD) of the largest subunit of RNA polymerase II in higher eukaryotes has an altered form in Leishmania donovani. To determine whether this is a general feature of the kinetoplastida and to investigate the role of this domain in parasitic RNA pol II transcription, we isolated the gene encoding RNA pol II LS (rpol IILS) and analyzed its C-terminal domain. The discreteness observed may be due to a functional constraint delineating parasite from host. The gene for L. donovani rpol IILS was picked up and sequenced. The CTD of L. donovani rpolIILS was purified as a His-tagged recombinant protein and phosphorylated with a crude kinase extract from L. donovani. An immunoblot analysis of the phosphorylated CTD and photo-crosslinked L. donovani nuclear extracts was done using anti-CTD antibody. The L. donovani rpol IILS is encoded by a single-copy gene. Its transcript is matured posttranscriptionally, with the mini-exon trans-spliced 397 bases upstream of the initiation site. The uniqueness of Leishmania rpol IILS CTD according to prediction analysis was corroborated with in vitro phosphorylation of the recombinant protein. Photoaffinity labelling of L. donovani nuclear run-on transcripts and immunoblot analysis using anti-CTD antibody could identify the active form of RNA polymerase II enzyme in this parasite. The L. donovani rpol IILS possesses a unique C-terminal extension lacking the characteristic repeats but containing serine residues as a potential phosphorylation site. Anti-CTD antiabody could recognize a single molecular species for the RNA pol II enzyme in L. donovani.


    Tytuł oryginału: A prospective-controlled study of pregnant veterinary staff exposed to inhaled anesthetics and X-rays.
    Autorzy: Shuhaiber Samaer, Einarson Adrienne, Radde Ingeborg C., Sarkar Moumita, Koren Gideon
    Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (4) s.363-373, tab., bibliogr. 29 poz.
    Sygnatura GBL: 306,313

    Hasła klasyfikacyjne GBL:
  • weterynaria
  • medycyna pracy

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca epidemiologiczna

    Wskaźnik treści:
  • ludzie
  • płód
  • płeć żeńska
  • ciąża

    Streszczenie angielskie: Most veterinary staff are women of reproductive age. They are exposed to "waste" anesthetic gas and ionizing radiation in their workplace, which may endanger fetal safety. Presently, exposure of female veterinary staff to these health hazard has not been adequately addressed in the medical literature. Our primary objective was to investigate the incidence of major malformations associated with occupational exposure to inhaled anesthetics and/or radiation among pregnant veterinary staff. The secondary objective was to determine the rates of other adverse outcomes. We prospectively collected data on and followed-up women occupationally exposed to inhaled anesthetics and/or radiation in veterinary practices in Ontario, and compared them to controls matched for maternal age and gestational age at the time of call to the Motherisk Program. A total of 95 women were prospectively enrolled and followed-up. Among the participants there were 87 (93.5 p.c.) and 88 (92.8 p.c.) liverbirths in the study and control groups, respectively. There were 4 (4.8 p.c.) major birth defects in the study group and 3 (3.4 p.c.) in the control group. The rates of spontaneous abortion were also similar, 6 (6.4 p.c.) cases in the study group and 7 (7.4 p.c.) cases in the cotnrol group. These resutls suggest that Ontario female veterinary staff exposed to inhaled anesthetics and/or radiation do not seem to be at an increased rosk for major malformations above baseline risk.

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