Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SĘKTAS
Liczba odnalezionych rekordów: 1



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku
1/1

Tytuł oryginału: Ekspresja genów klonowanych w wektorach plazmidowych w zrekombinowanych szczepach Escherichia coli.
Tytuł angielski: Expresion of genes cloned in plasmid vectors in recombinant Escherichia coli strains.
Autorzy: Sęktas Marian
Źródło: Kosmos 2002: 51 (3) s.365-373, il., tab., bibliogr. [25] poz., sum.
Sygnatura GBL: 313,370

Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Streszczenie angielskie: Use of Escherichia coli bacteria as a host for high-level expression of cloned genes has become common. The purfication of a recombinant protein is greatly accelerated if the protein can be isolated from cells that overproduce it. To maximize expression, the cloned gene must be transcribed and translated as efficiently as possible. This is possible due to the construction of expression vectors, modified plasmids with useful features, which can be propagated and controlled in special hosts (expression systems). Usually, vectors for cloning and expressing targer-DNA are derived from medium-copy plasmids like pBR322. E. coli expression systems should meet several criteria including (i) minimal basal expression of the gene to be expressed under repressed conditions, (ii) fast and uncomplicated induction of a wide variety of genes to a high level of expression, and (iii) easy cloning and DNA manipulation features. This article describes how the most common T7 expression system, derived from bacteriophage T7, functions The system consists of a plasmid vector that allows coloning of the target DNA under T7 promoter control, and the T7 RNA polymerase gene borne by the recombinant bacterial host. The system is capable of expressing a wide variety of DNAs from prokaryotic and eukaryotic sources. In principle, the T7 system can be completely selective because host RNA polymerase and phage's polymerase recognized different promoters. However, synthesis of recombinant proteins, especially those that are toxic to the host, must be controlled, being at zero or non-toxic levels...

    stosując format: