Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: RUSZCZYŃSKA
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Tytuł oryginału: Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues.
Autorzy: Stachelska Alicja, Wieczorek Zbigniew, Ruszczyńska Katarzyna, Stolarski Ryszard, Pietrzak Monika, Lamphear Barry J., Rhoads Robert E., Darżynkiewicz Edward, Jankowska-Anyszka Marzena
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.671-682, il., tab., bibliogr. s. 679-682
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • genetyka

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: Translation initiation factor eIF4E binds the m**7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70 p.c. of genes express mRNAs with an unusual cap structure containing m3ý,ý,**7 G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m**7 GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m**7 G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m**7 G- and m3ý,ý,**7 G-containing caps (Kas 2x10**6 M**-1 to 7x10**6 M**-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of M**7 G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 ph units than that of eIF4E from human erythrocytes. these results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.

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    Tytuł oryginału: Technetium-99m MIBI imaging in diagnosis of pelvic and abdominal masses in patients with suspected gynaecological malignancy.
    Autorzy: Królicki Leszek, Ćwikła Jarosław B., Timorek Agnieszka, Ruszczyńska Małgorzata, Stelmachow Jerzy, Sawicki Włodzimierz, Kolasińska Agnieszka D., Kupryjańczyk Jolanta, Lieberman Gidon, Buscombe John R.
    Źródło: Nucl. Med. Rev. 2002: 5 (2) s.131-137, il., tab., bibliogr. 28 poz.
    Sygnatura GBL: 313,603

    Hasła klasyfikacyjne GBL:
  • pediatria
  • ginekologia i położnictwo
  • onkologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Background: The aim of this study was to determine the diagnostic value of 99mTcMIBI planar dynamic scintigraphy in the diagnosis of gynaecological malignancies and to determine if it has a greater diagnostic accuracy than conventional ultrasound technique (US). Material and methods; A prospective trial was performed to assess the accuracy of 99mTcMIBI scintigraphy and ultrasound in 93 female patients (mean age 50, SD 16; range 17-82 years). Three readers assessed all the imaging independently and this was comparedd with histological eamination in 89 cases and by clinical follow-up and correlative imaging for a minimum of 6 months in 4 patientss. Results: There were 37 patients with cancer of gynaecological origin. Tehre were in addition 56 benign lesions within the pelvis. 99mTcMIBI identified correctly 29 of the 337 malignant tumours localised within the pelvis and also correctly identified 21 of 23 metastases within the abdomen. Conventional US identified correctly 35 of the 37 tumours in the pelvis and 16 sites of metastsaes within the abdomen. The sensitivity and specificity of tumour detection within the pelvis for 99mTcMIBI were as follows: 78 p.c. and 70 p.c.; and for metastases within the abdomen 91 p.c. and 90 p.c. The results of ultrasound for tumour detection within the pelvis: the sensitivity and specificity were 95 p.c. and 79 p.c. and abdominal metastases 70 p.c. and 97 p.c. Analysis of the index area under a receiver operator characteristic (ROC) curve in scintigraphy did not show a ...

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