Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: RUSSEL
Liczba odnalezionych rekordów: 2



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku
1/2

Tytuł oryginału: Detection of gsp somatic mutation through direct sequencing of heteroduplex alleles disclosed by denaturing gradient gel electrophoresis.
Autorzy: Fragoso Maria C. Barisson Villares, Lado Valeria S., Latronico Ana C., Frazzatto Eliana T. Salgado, Russell Alan J., Mendonca Berenice B.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR15-BR18, il., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • genetyka
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: The identification of somatic mutations in tissues is often difficult when the number of normal alleles in the tissue far exceeds the number of mutation was not possible by direct sequencing and present a new approach that improves the identification of gsp somatic mutations. Genomic DNA was extracted from frozen tissue of a human ovarian stromal Leydig cell tumor. Exons 8 and 9 of the Gsŕ gene were amplified by PCR and despite the abnormal migration pattern at this first DGGE, direct sequencing of the PCR product did not reveal mutations, probably due to the small amonunt of mutant alleles. To improve this amount, the PCR products were re-amplified using as template the excised products of the mutant homoduplex and heteroduplex bands obtained at the first DGGE. This approach resulted in the enhancing of the mutant homoduplex bands whereas the heteroduplex bands remained unchanged at the second DGGE. Direct sequencing of the second round PCR clearly identified the mutation R201C in the ovarian Leydig cell tumor. We have demonstrated a relatively rapid, convenient and reliable method to improve gsp somatic mutation detection combining a second DGGE of the PCR products obtained from the heteroduplexes and mutant homoduplex bands disclosed in a first DGGE followed by direct sequencing.

    2/2

    Tytuł oryginału: Laypersons' expectation of the sequelae of whiplash injury. A cross-cultural comparative study between Canada and Lithuania.
    Autorzy: Ferrari Robert, Obelieniene Diana, Russel Anthony Science, Darlington Paul, Gervais Roger, Green Paul
    Źródło: Med. Sci. Monitor 2002: 8 (11) s.CR728-CR734, tab., bibliogr. 21 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca epidemiologiczna

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background: The objective of the present study is to compare the frequency and nature of expected 'whiplash' symptoms in Lithuania (a country where the late whiplash syndrome is rare or unknown) with that in Canada. Material/Methods: A symptom checklist was administered to 2 subject groups selected from local companies in Kaunas, Lithuania, and Edmonton, Canada, respectively. Subjects were asked to imagine having suffered a neck sprain (whiplash injry) with no loss of consciousness in a motor vehicle accident, and to check which, of a variety of symptoms, they would expect might arise from the injury. For symptoms they anticipated, they were asked to select the period of time they expected those symptoms to persist. Results: In both the Lithuanian and Edmontonian groups, the pattern of symptoms anticipated closely resembled the acute symptoms commonly reported by accident victims with acute neck sprain, but while up to 50 p.c. of Edmontonians also anticipated symptoms to last months or years, very fe Lithuanian subjects selected any symptoms as likely to persist. Conclusions: In Lithuania, despite the documented occurrence of neck sprain symptoms in some 50 p.c. of individuals following motor vehicle accidents, there is a very low rate of expectation of any sequelae from this injury. What current or previous aspects of society that underlie this remain uncertain. This lack of expectation of chronicity in Lithuania may, in part, determine the low prevalence of the late whiplash syndrome there. Further sutdies of symptom expectation as an etiologic factor in the late whiplash syndrome are needed.

    stosując format: