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Tytuł oryginału: Acta Microbiologica Polonica
Tytuł angielski: Green fluorescent protein as a molecular marker in microbiology.
Autorzy: Rosochacki Stanisław Józef, Matejczyk Marzena
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.205-216, il., tab., bibliogr. s. 214-216
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Typ dokumentu:
  • tytuł obcojęzyczny

    Streszczenie angielskie: Molecular markers such as: lacZ(b-galactosidase), xylE (catechol 2,3-dioxygenase), lux (bacterial luciferase), luc (insect luciferase), phoA (alkaline phosphatase), gusA and gur AA (á-glucuronidase), gfp (green fluorescent protein), bla (á-lactamase) and other antibiotic resistance markers, heavy metals resistance genes are commonly used in environmental microorganisms research (Errampalli et al., 1998; Kohler et al., 1999). Most of these markers require one or more substrates, complex media and/or expensive equipment for detection. The gfp gene is widely used as a marker because of its very useful properteis such as high stability, minimal toxicity, non-invasive detection and the ability to generate tha green light without addition of external cofactors and without application of expensive equipment. Various applications of that reporter gene were showed starting from onitoring of microorganism's survival in complex biological systems such as activatedsludge to biodegradation of chemical compounds in soil. GFP allowed the detection, determination of spatial location and enumeration of bacterial cells from diverse environmental samples such as biofilm and water. The gfp as a biomarker was very useful in monitoring of gene expression and protein localisation in bacterial cells, too. The techniques with using gfp marker promise to supply a better understanding of environmental processes. It can make possible to use that knowledge in designing more effective and more efficient methods of biodegradation of toxic compounds from different envirnonments.

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