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Tytuł oryginału:
Characterization of dendritic cells generated in vivo by an E. coli derived chimeric dual receptor agonist.
Autorzy:
Kahn
Larry E.,
Doshi
Parul,
McGarity
Kelly L.,
Yu
Minfu,
Bono
Christine P.,
Lie
Wen-Rong,
Rankin
Anne M.,
Smidt
Mary L.,
Streeter
Philip R.,
Klein
Barbara K.,
Welply
Joseph K.,
Woulfe
Susan L.
Źródło:
Med. Sci. Monitor 2002: 8 (12) s.BR504-BR514, il., tab., bibliogr. 48 poz.
Sygnatura GBL:
313,278
Hasła klasyfikacyjne GBL:
immunologia
Typ dokumentu:
praca doświadczalna
tytuł obcojęzyczny
Wskaźnik treści:
zwierzęta
myszy
płeć żeńska
Streszczenie angielskie:
Background: Progenipoietin-4 (ProGP-4) is an E. coli derived chimeric growth factor that activates the human Flt3 and G-CSF receptors. ProGP-4 possesses cross-species activity and treatment of mice with ProGP-4 results in increas in the number of WBC and Class II+/CD11c+ cells in both spleen and peripheral blood. Herein, we report morphologic, phenotypic and functional evaluation of Class II+/CD11c+ cells generated by in vivo administration of ProGP-4. Material and methods: C57BL/6 mice were injected daily with ProGP for 7 to 18 days. Leukocytes from spleen and peripheral blood were analyzed by flow cytometry to enumerate and characterize changes in DC populations. Spleens from ProGP treated mice were evaluated by immunocytochemistry and enriched CD11c+ populations were functionally assessed in a mixed lymphocyte assay and in an antigen dependent CTL assay. Results: Administration of this dual receptor agonist to mice resulted in dose-dependent increases in the numbers of total white blood cells and Class II+/CD11c+ cells in spleen and peripheral blood. CD11c+ cells from ProGP-4 treated mice co-expressed DEC205 and also expressed CD80, CD86 and CD40, albert at lower levels as compared to Class II+/CD11c+ cells from untreated animals. Despite lower co-stimulatory molecule expression, PorGP-4 generated ClassII+/CD11c+ cells stimulated proliferation of allogenic T cells and an antigen-specific T cell hybridoma as efficiently as bone marrow derived dendritic cells from untreated mice. Conclusion: The data presented in this report highlight the ability of E. coli derived...
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