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Tytuł oryginału: Indoor fungi and their ciliostatic metabolites.
Autorzy: Pieckova Elena, Kunova Zuzana
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.59-63, tab., bibliogr. 31 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:
  • toksykologia
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • zarodki

    Streszczenie angielskie: According to epidemiological studies, it is possible that some secondary metabolites of indoor airborne fungi could be responsible for health troubles which occupants sufer from. In our previous experiments, a model with tracheal rings of 1-day-old chicks in vitro was shown to be a very suitable method to study the ciliostatic chloroform-extractable endo- end/or exometabolites of filamentous fungi. In this study we isolated the filamentous fungi from walls of "moudy" dwellings and schools (cultivation on dichloran 18 p.c. glycaerol agar at 25 and 37řC for 10 d) in Slovakia. We studied the ciliostatic effect of the chloroform-extractable endo- and exometabolites of 96 representative isolates (Stationary cultivation on the liquid medium with 2 p.c. of yeast extract and 10 p.c. of sucrose at 25řC for 10 days) on the cilia movement in tracheal organ cultures of 1-day-old chickens in vitro after 24, 48 and 72 hrs (incubation in the minimal essential medium according to Eagle with Earl's salts and 20 ćg of extract of metabolites dissolved in dimethylsulfoxide per 1 mL). Strains of Penicillium Link: Fr. sp., Aspregillus versicolor (Vuill.). Tiraboschi, A. flavus Link, Cladosporium sphaerospermum Penzig adn C. cladosporioides (Fres.) de Vries were isolated most frequently. Two A. flavus isolates were able to produce aflatoxins B1, B2, G1, G2 in vitro after cultivation on the liquid medium with 20 p.c. sucrose and 2 p.c. yeast extract. This is the first isolation of aflatoxigenic A. flavus strains from dwellings in Slovakia. All frequently isolated strainsproduced secondary metabolites...

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