Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: Neuroprotective effect of garlic compounds in amyloid-á peptide-induced apoptosis in vitro.
Autorzy: Peng Qiaoling, Buz'Zard Amber R., Lau Benjamin H. S.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR328-BR337, il., bibliogr. 60 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: Neuronal apoptosis is one of the pathoglogical features of Alzheimer's disease (AD) and is associated with senile plaques containing amyloid-á peptide (Aá). Reactive oxygen species (ROS)are proposed to be involved in the apoptotic mechanism of Aá-mediated neurotoxicity. The purpose of this study was to determine the effects of aged garlic extract (AGE) and S-allyl cysteine (SAC) on Aá25-35-induced apoptosis and ROS generation in a rat pheochromocytoma (PC12) cell line. Material/Methods: PC12 cells were grown in RPMI 1640 medium containing 5 p.c. fetal calf serum and 10 p.c. horse serum. Cells were incubated wtih AGE or SAC for 24 h prior to exposure to Aá25-35 for various times. Cell viability, DNA fragmentation, number of apoptotic cells, caspase activity and generation of ROS were determined. Results: Aá25-35-induced apoptosis in PC12 cells was demonstrated by: 1) A dose-dependent loss of cell viability; 2) A time- and dose-dependent increase in apoptotic cells; 3) An induction of DNA fragmentation; and 4) An increase in caspase-3 activity and cleavage of poly (ADP-ribose) polymerase (PARP). After exposing PC12 cells to Aá25-35, a significant increase in ROS preeceded apoptotic events. AGE and SAC not only suppressed the generation of ROS but also attenuated caspase-3 activation, DNA fragmentation, PARP cleavage and eventually protected against Aá-induced apoptosis. Conclusions: Our data suggest that ROS may be involved in Aá-induced apoptosis in PC12 cells. They further suggest that garlic compounds can reduce apoptosis, possibly by enhancing the endogeous antioxidant defenses.

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    Tytuł oryginału: Comparison of 99mTC-alafosfalin and 67Ga-citrate in a mouse model of bacterial infection.
    Autorzy: Tsopelas Chris, Penglis Stan, Bartholomeusz F. Dylan L.
    Źródło: Nucl. Med. Rev. 2002: 5 (2) s.93-97, il., tab., bibliogr. 18 poz.
    Sygnatura GBL: 313,603

    Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy
  • płeć żeńska

    Streszczenie angielskie: Background: The antibiotic-peptide 99mTC-alafosfalin was assessed as an infection imaging agent in Staphylococcus aureas infected mice by comparison with 99mGa-citrate, and also examined for influence of septic status on tracer biodistribution by comparison with normal mice. Material and methods: Intramuscular doses of S. aureus were administered into the right thigh muscle of mice and the infection was allowed to develop for 20 hours. In separate experiments, 99mTC-alafosfalin and 67Ga-citrate were subsequently administered adn allowed to localise. Quantitative organ distribution sstudies were performed in conjunction with scintigrapic images at 1 and 4 hours posst injection. An additional biodistribution with 99mTC-alafosfalin in normal mice was also performed. Results: 99mTC-alafosfalin was predominantly renal excreted, with low liver, intestine and bone uptake. Tehre was no difference in the uptake of these organs when infected mice were compared with normal mice. 99mTC-alafosfalin activity in the intestine at 1 and 4 hours was substantially less than 67Ga-citrate. For 99mTC-alafosfalin, infected/non-infected thigh ratios of 2.8/1.0 and 3.6/1.0 were determined at 1 and 4 hours post injection resepectively. 67Ga-citrate gave ratios of 1.6/11.0 and 3.7/1.0 at teh corresponding time points. Conclusions: 99mTC-alafosfalin uptake was mnore rapid than 67Ga-citrate, yet diffuse at the infectious sites in mice. The small and juvenile mouse model resulted in uptake of the phosphonic acid tracer by active ...

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