Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: PARKHOMENKO
Liczba odnalezionych rekordów: 2



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku
1/2

Tytuł oryginału: Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. P. 1: Spectrofluorometric analysis.
Autorzy: Parkhomenko Tatiana V., Klicenko Oksana A., Shavlovski Mikhail M., Kuznetsova Irina M., Uversky Vladimir N., Turoverov Konstantin K.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR261-BR265, il., bibliogr. 35 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Background: Serum albumin (SA) is a major proteinacous component of the blood plasma. In the present study we report on the determination and structural characterization of the modified SA in the plasma of glomeruklonephritis (GN) patients. Fluorescence analysis of albumin-enriched fractions (AEF) isolated from serum of GN patients leads to the discovery of a proteinacous component with essential blue shift of intrinsic fluorescence. Material/Methods: AEF were isolated from serum blood of 35 GN patietns, 30 GN patietns with chronic renal failure (CRF) corrected by hemodialysis (HD) and 40 healthy donors. Their fluorescence chacteristics have been compared using variations in teh value of parameter A = I320/I365 that characterizes fluorescence spectrum position and shape. Results: The fluorescence spectra of AEF from GN and GN with CRF patient were blue-and red-shifted, respectively, in comparison with spectra of donors' serum. The value of parameter A was 1.27 ń 0.05 for protein preparateions from healthy donors, while this characteristic lies within range of 1.3-2.1 or 0.77 - 1.29 for Gn and Gn with CRF patients, resectively. Moreover, for GN patients, the magnitude of spectral parameter A was approaching control values during the treatment of disease. Conclusion: hSA enriched fractions isolated from GN patients or GN patients with CRF corrected by HD differ by their spectrofluorometric properties from those of healthy donors. This technique allows the detection of renal disease and the efficiency fo thier cure.

    2/2

    Tytuł oryginału: Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. P. 2: Evidence for the enhancement of the haptoglobin. Level at the patholgoical conditions.
    Autorzy: Parkhomenko Tatiana V., Klicenko Oxana A., Shavlovski Mikhail M., Poletaev Andrey I., Kuznetsova Irina M., Uversky Vladimir N., Turoverov Konstantin K.
    Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR266-BR271, il., bibliogr. 59 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Background: Human serum albumin (hSA), the major component in blood plasma, fulfills a fundamental biological role as a universal carrier and resevoir in blood plasma, tissues, and secretions. Becaues of essential diversity in the bound ligands, hSA was shown to possess significant structural divergence. In the first paper of this series we reported on that albumin-enriched fractions isolated from serum of glomerulonephritis (GN) patients possesses essential blue shift of intrinsic fluorescence. Identification of this component was the major goal of this study. Material/Methods: Partially purifies SA preparations were isolated from blood plasma of 19 GN patients and 8 healthy donors. These preparations have been analyzed by combination of chromatographic (gel-filtration and hydrophobic sorption) and spectrofluorometric techniques. Results: At least six differented albumin enriched fractions were isolated from blood of GN pateitns. High molecular weight protein fraction with blue shifted intrinsic fluorescence spectrum was segregated. Higher concentration of this fraction may be responsible for higher parameter A values, which is characteristic of protein preparations from GN patietns. Amino acid sequence showed that á-haptoglobin is one of the components of this fraction. Conclusion: Combination of chromatographic methods with monitoring intrinsic fluorescence spectra of albumin-enriched fractions from blood plasma of healthy donors and GN patients represents a useful tool for the description of hSA heterogeneity and for the detection of the changes in the level of disease-related compounds. This approach is also appropriate for the isolation of individual components, different in their parameter A values, from the blood plasma.

    stosując format: