Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: The effect of Arg209 to Lys mutation in mouse thymidylate synthase.
Autorzy: Cieśla Joanna, Gołos Barbara, Wałajtys-Rode Elżbieta, Jagielska Elżbieta, Płucienniczak Andrzej, Rode Wojciech
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.651-658, il., tab., bibliogr. s. 657-658
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • genetyka
  • onkologia
  • hematologia
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Mouse thymidylate synthase R209K (a mutation corresponding to R218K in Lactobacillus casei), overexpressed in thymidylate synthase-deficient Escherichia coli strain, was poorly soluble and with only feeble enzyme activity. The mutated protein, incubated with FdUMP and N5,10 -methylenetetrahydrofolate, did not form a complex stable under conditions of SDS/polyacrylamide gel electrophoresis. The reaction catalyzed by the R209K enzyme (studied in a crude extract), compared to that catalyzed by purified wild-type recombinant mouse thymidylate synthase, showed the Km value for dUMP 571-fold higher and Vmax value over 50-fold (assuming that the mutated enzyme constituted 20 p.c. of total crude extract protein) lower. Thus the ratios kcat, R209K/kcat, 'wild' and (kcat, R209K/Km, R209K**dUMP)/(kcat, 'wild'/Km, 'wild'**dUMP) were 0.019 and 0.000032, respectively, documenting that mouse thymidylate synthase R209, similar to the corresponding L. casei R218, is essential for both dUMP binding and enzyme reaction.

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    Tytuł oryginału: Integrony.
    Tytuł angielski: Integrons.
    Autorzy: Wolinowska Renata, Masny Aleksander, Płucienniczak Andrzej
    Źródło: Kosmos 2002: 51 (3) s.353-364, il., tab., bibliogr. [19] poz., sum.
    Sygnatura GBL: 313,370

    Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Streszczenie angielskie: Accumulation of antibiotic resistance among pathogenic bacteria has called attention to horizontal gene transfer that involves plasmids and transpozons. Integrons, usually placed on mobile genome elements, are very deeply engaged in the process of origin of multiple-drug-resistant strains. Integrons are genetic elements that contain determinants of the components of the site-specific recombination system that recognizes and captures mobile gene cassettes. More than 70 different antibiotic resistnce genes covering most classes of antimicrobials presently in use have been detected in gene cassettes. Integrons are frequently found in clinical and environmental strains of gram-negative rods. The discovery of super-integrons, i.e. genetic structures gathering gene cassettes in a hunge number, led to the conception of genome cassettes capture as an element of a broader phenomenon of bacterial genome modification in response to changing environmental conditions.

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