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Zapytanie: ORCHEL
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Tytuł oryginału: Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment.
Autorzy: Dzierżewicz Zofia, Orchel Arkadiusz, Węglarz Ludmiła, Latocha Małgorzata, Wilczok Tadeusz
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.211-220, il., bibliogr. 23 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • farmacja
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Gut-derived adenocarcinoa Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on proliferation, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secretion. Differentiation was determined by brush border alkaline phosphoatase activity. Apoptosis was assessed by acridine orane and Hoechst stains. Differentiation and apoptosis were analyzed in both adherent and floating cell populations. The transformed Caco-2 cells did not retain their malignant phenotype in the presence of NaB. They appeared to undergo a change in the phenotype induced by NaB, as indicated by reduced proliferation, enhanced differentiation, stimulation of apoptosis leading to decreased viablility of cells, and stimulaiton of interleukin-8-secretion. Considering all the above facts and data, we postulate that Caco-2 cells cultured in NaB supplemented medium could regain the phenotypic characteirstics of the phenotype of the parent cell from which originated the caco-2 line.

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