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Tytuł oryginału: Advances in immunocytochemistry.
Autorzy: Noorden Susan Van
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.121-124, il., bibliogr. 20 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:
  • immunologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • zwierzęta
  • myszy

    Streszczenie angielskie: Recent advances in immunohisto/cytochemical methods have been directed towards new fluorescent labaels, and to increasing sensitivity and improving methods for multiple immunostaining. The newestfluorescent dyes come in many colours, are much mosre stable than fluorescein isothiocyanate and give brighter fluorescence. The greatest impact on sensitivity comes from heat-induced antigen retreival on paraffin sections. Biotinylasted tyramide can also increase enormously the amount of label on a preparation. The immunogold method with silver enhancement has been improved by nanogold, which is smaller than colloidal gold, attracts more silfer and increases the sensitivity even further. The difficulties of multiple labelling with two or more primary antibodies raised in the same species due to cross-binding between reagents have largely been overcome. There are new methods of blocking spare reactive sites on the first reaction by strong heat, e. g. from microwaving in buffer. Alternatively, one of the antibodis can be directrly biotinylated very easily with a new commercially available kit.

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