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MORTON
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Tytuł oryginału:
Fibrinogen/fibrin and albumin colocalize on the surface of placental villi - a novel element of feto-maternal barrier?
Autorzy:
Wasiutyński
Aleksander,
Wilczyński
Grzegorz M.,
Morton
Marcin,
Koperski
Łukasz,
Górnicka
Barbara,
Ziarkiewicz-Wróblewska
Bogna
Źródło:
Ann. Diagn. Paediatr. Pathol. 2002: 6 (3/4) s.125-129, il., bibliogr. 14 poz.
Sygnatura GBL:
313,395
Hasła klasyfikacyjne GBL:
immunologia
ginekologia i położnictwo
Typ dokumentu:
praca kliniczna
tytuł obcojęzyczny
Wskaźnik treści:
ludzie
dorośli 19-44 r.ż.
płeć żeńska
in vitro
Streszczenie angielskie:
The phenomenon of fetal escape from maternal anti-allogenic immune response is not completely understood. It was hypothesized that fibrininogen/fibrin and albumin, derived from plasma, can form on the surfaces of various cells, a complex, which is able to prevent the access of immune-competent cells and molecules. In this report, we have investigated, using immunohistochemical approach, whether such a complex could exist on the surface of villi in the human placenta. By means of both peroxidase-based and fluorescent techniques, we have immunolocalized fibrinogen/fibrin and albumin in paraffin sections of normal termplacentas, obtained upon delivery from 11 women. We found marked deposition of both fibrinogen/fibrin and albumin around the villi, in the form of dense layers covering respectively 78,4 ń 1,4 p.c. and 82,4 ń 1,2 p.c. of the villous surface. At those sites, both immunoreactivities strictly colocalized, as revealed by doublelabel immunofluorescent confocal microscopy. The analysis of fluorescence-resonance energy transfer (FRET) between fluorophores indicated that a substantial portion of fibrinogen/fibrin and albumin molecules on the villous surface are separated from each other by the distance smaller or equal to 30nm. Our results the presence of an inter-molecular complex between fibrinogen/fibrin and albumin on the surface of human trophoblast, which might constitute a novel element of a protective barrier at the feto-maternal interface.
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