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Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets.
Exp. Hematol. 2002: 30 (7) s.751-760, il., tab., bibliogr. 51 poz.
Hasła klasyfikacyjne GBL:
praca opublikowana za granicą
Objective: The development of megakaryocytes is regulated by thrombopoietin (TPO), which binds to the c-mpl receptor, and by several other cytokines such as interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), cilliary neurotropic factor (CNTF), and oncostatin (OSM), which bind to gp130 protein-coupled receptors. We attempted to identify signal transduction pathways activated by these factors in normal human megakaryocytes. Materials and methods: To better understand the role of these factors in normal human megakaryopoiesis we studied their effect on 1) purified human bone marrow-derived CD34+ cells, 2) human ŕIIbá3+ cells (shown by immunophenotypical and morphological criteria to be megakaryoblasts), which had been expanded ex vivo from CD34+ cells in chemically defined artificial serum, and 3) gel-filtered human peripheral blood platelets. Further, in an attempt to correlate the influence of these factors on cell proliferation and survival activation of signal transduction pathways, we evaluated their effect on the phosphorylation of MAPK p42/44 and activation of PI-3K-AKT and JAK-STAT proteins in these various cell types. Results: Using serum-free liquid cultures, we found that only TPO and IL-6 protected CD34+ cells and megakaryocytes from undergoing apoptosis (decrease in annexin-V binding, PARP cleavage, and activation of caspase-3). Moreover, only TPO when used alone and IL-6 only when used in combination with TPO, stimulated the growth of human colony-forming unitmegakarocytes (CFU-Meg) in semisolid serum-free medium...
Comparison of three instruments for assessing ongoing intimate partner violence.
Med. Sci. Monitor 2002: 8 (3) s.CR197-CR201, il., tab., bibliogr. 23 poz.
dorośli 19-44 r.ż.
dorośli 45-64 r.ż.
Background: Acitve injury surveillance programs need to address 'ongoing' intimate partner violence (IPV). While the Abuse Assessment Screen (AAS) has been validated for 'present' (within a year) IPV it is not clear that it is valid for 'present' (ongoing) IPV. We have created an OAS (Ongoing Abuse Screen, OAS) by changing the AAS to specifically request information related to 'ongoing' IPV. The hypothesis of this study was that the OAS represents a construct that is different from either the original AAS or a single question asking about ongoing IPV. Material/Methods: All patietns presenting to the ED during a convenience sampling of shifts completed the survey. The survey included the OAS, the AAS, and the question "Are you presently a victim of IPV?' Comparisons were made between these 3 using the kappa statistic for agreement. Results: A total of 488 surveys were completed. The AAS was positive in 288/488 (59 p.c., 95 p.c. CI = 55-63 p.c.), the OAS was positive in 78/488 (16 p.c., 95 p.c. CI = 13-19 p.c.), and the single question for DV was positive in 14/488 (3 p.c., 95 p.c. CI - 2-5 p.c.). Kappa was 0.28 for the AAS and the OAS. When compared to the single question about present DV, kappa was 0.05 for the AAS and 0.27 for the OAS. Conclusion: The OAS may be a useful tool for evaluating ongoing IPV. The OAS resulted in rates different from that of the AAS and may be more specific to ongoing IPV than the AAS and more sensitive than a single question about DV.
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