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Estrogen receptor ŕ and á expression in theca and granulosa cells from women with polycystic ovary syndrome.
J. Clin. Endocrinol. Metab. 2002: 87 (12) s.5532-5538, il., bibliogr. 40 poz.
Hasła klasyfikacyjne GBL:
ginekologia i położnictwo
praca opublikowana za granicą
A defining characteristic of dominant follicles is high estradiol concentrations. Abnormal expression of estrogen receptors (ERs) could contribute to poor follicular develoment and ovulatory failure in polycystic ovary syndrome (PCOS). The aim of this study was to determine whether there are differences in ERŕ and ERá expression in granulosa cells (GC) and theca cells (TC) from women with PCOS, compared with regulary cycling women. GC and TC were obtained by microdissection from 12 polycystic and 23 normal ovaries. ERŕ and ERá mRNA and protein expression were measured by semiquantitative RT-PCR and Western blot, respectively. In control ovaries, both GC and TC ERŕ mRNAs were higher in small antral (SA) than in dominant follicles. ERŕ mRNA was similar in PCOS and size-matched control follicles. In control follicles, ERŕ protein concentrations were higher in GC than in TC. In GC, the ERŕ concentrations were comparable among SA, dominant, and PCOS follicles. In TC, ERŕ concentrations were lower in dominant follicles but were markedly increased in PCOS. In control ovaries, GC and TC expression of ERá mRNA was higher in SA, compared with dominant follicles. In PCOS, ERá mRNA was intermediate between SA and dominant follicles in both GC amnd TC. In GC, the ERá protein concentrations followed the same pattern as mRNA expression; but in TC ERá, protein in PCOS was equivalent to that in dominant follicles. The results of this study demonstrate that there are significamnt alterations in the expression of ERŕ and ERá in PCOS that may be related to abnormal follicular development.
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