Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: LUTSIK
Liczba odnalezionych rekordów: 1



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku
1/1

Tytuł oryginału: In vitro studies of activation of phagocytic cells by bioactive peptides.
Autorzy: Stoika R. S., Lutsik M. D., Barska M. L., Tsyrulnyk A. A., Kashchak N. I.
Źródło: J. Physiol. Pharmacol. 2002: 53 (4 p. 1) s.675-688, il., bibliogr. 22 poz.
Sygnatura GBL: 302,092

Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: The effect of formyl chemotactic peptide (fCTP, fMet-Leu-Phe), á-amyloid peptides (á-AP, 1-42, 1-16 and 25-35), and bradykinin (BK) on functional activity of phagocytic cells has ben investigated. Wheat germ agglutinin (WGA) was also used as a model membrane binding agent of polypeptide nature. Murine monocytemacrophage cell line J774.2 and normal human blood polymorphonuclear (PMN) cells were used as target phagocytic cells. Their activity was quantitatively estimated by measuring phagocytosis of killed yeast cells. á-AP (1-41) maximally stimulated phagocytosis at 0.1 ćg/ml, ml, BK - at 1.0 ćg /ml, and fCTP - at 2.0 ćg/ml. á-AP (1-16) and á-AP (25-35) were inactive in used test-systems. Phagocytosis-inducing activity of á-AP (1-42) and BK reached maximal levels in 2 h and decreased after 4-6 h of incubation. Phagocytosis numbers were compared with the indicators of phagocytic cell activation, such as absorption of neutral red dye, glucose utilization, production of super-oxide anion (NBT-test) and nitrite accumulation (indicator of NO production). NBT-test, which may be related to the killing ability of phagocytic cells towards the ingested objects, was positive only in stimulated PMN leukocytes, while the nitrite accumulation was detected only in stimulated macrophages. Nitrite accumulation in macrophages was markedly induced by lipopolysaccharide and to a lower extent by 0.5 ćg/ml á-AP (1-42). In high dose (5.0 ćg/ml) á-AP suppressed nitrite accumulation in macrophages stimulated by lipopolysaccharide ...

    stosując format: