Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL
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KROTKIEWSKA
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Tytuł oryginału:
Interaction of glycophorin A with lectins as measured by surface plasmon resonance (SPR).
Autorzy:
Krotkiewska
Bożena,
Pasek
Marta,
Krotkiewski
Hubert
Źródło:
Acta Bioch. Pol. 2002: 49 (2) s.481-490, il., tab., bibliogr. s. 489-490
Sygnatura GBL:
303,116
Typ dokumentu:
tytuł obcojęzyczny
praca doświadczalna
Streszczenie angielskie:
Glycophorin A (GPA), the major sialoglycoprotien of the human erythrocyte membrane, was isolated from erythrocytes of healthy individuals of blood groups A, B and O using phenol-water extraction of erythrocyte membranes. interaction of individual GPA samples with three lectins (Psathyrella velutina lectin, PVL; Triticum vulgaris lectin, WGA and Sambucus nigra I agglutinin SNA-I) was analyzed using a BIAcore**TM biosensor equipped with a surface plasmon resonance (SPR) detector. The experiments showed no substantial differences in the interaction between native and desialylated GPA samples originating from erythrocytes of either blood group and each of the lectins. Desialylated samples reacted weaker than the native ones with all three lectins. PVL reacted about 50-fold more srongly than wGA which, similar to PVL, recognized GlcNAc and Neu5Ac residues. SNA-I lectin, recognizing ŕ2-6 linked Neu5Ac residues, showed relatively weak reaction with native and only residual reaction with desialylated GPA samples. The data obtained show that SPR is a valuable method to determine interaction of glycoproteins with lectins, which potentially can be used to detect differences in the carbohydrate moiety of individual glycoprotein samples.
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