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Tytuł oryginału: Adhesive properties of cacinoembryonic antigen glycoforms expressed in glycosylation-deficient Chinese hamster ovary cell lines.
Autorzy: Krop-Watorek Anna, Kłopocki Arkadiusz G., Czerwiński Marcin, Lisowska Elwira
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.273-283, il., bibliogr. s. 280-283
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • immunologia
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • chomiki
  • płeć żeńska
  • in vitro

    Streszczenie angielskie: Carcinoembryonic antigen (CEA) is an oncofoetal cell surface glycoprotein that serves as an important tumour marker for colorectal and some other carcinomas. Its immunoglobulin-like structure places CEA within the immunoglobulin superfamily. CEA functions in several biological roles including homotypic and heterotypic (with other CEA familiy members) cell adhesion. Cell-cell interaction can be modulated by different factors, e.g., post-transplational modifications such as glycosylation. The purpose of this study was to examine whether changes in carbohydrate composition of CEA oligosaccharides can influence homotypic (CEA-CEA) interactions. In order to modulate glycosylation of CEA we used two different glycosylation mutants of Chinese hamster ovary (CH)) cells, Lec2 and Lec8. Lec2 cells showuld produce CEA with nonsialylated N-glycans, while Lec8 cells should yield more truncated sugar structures than Lec2. Parental CHO (Pro5) cells and the glycosylation deficient mutants were stably transfected wtih CEA cDNA. All three CEA glycoforms, tested in a solid-phase cell adhesion assay, showed an ability to mediate CEA-dependent cell adhesion, and no qualitative differences in the adhesion between the glycoforms were observed. Thus, it may be assumed that carbohydrates do not plas a role in homotypic adhesion, and the interactions between CEA molecules depend solely on the polypeptide structure.

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