Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: KOWIAŃSKI
Liczba odnalezionych rekordów: 2



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Tytuł oryginału: Relation between the age of specimen and the shrinkage of brain frozen sections.
Autorzy: Dziewiątkowski J[erzy], Wójcik S., Spodnik J[anusz] H., Spodnik E., Kowiański P[rzemysław, Moryś J[anusz]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.115-116, il., bibliogr. 13 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Typ dokumentu:
  • praca związana ze zjazdem
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • króliki

    Streszczenie angielskie: The aim of the study was to assess the effect of age of the animal upon the real thickness of the frozen sections. The study was performed on 19 rabbit brains. The thickness of the frozen sections regardless of their staining is age-dependent. The relation is proportional during the period from 7 to 180 postnatal day and characterizes both immunohistochemical as well as cresyl violet-stained sections; moreover, changes of the section thickness proceed parallely. It is suggeted that especially for some stereological parameters all required procedures should be standardized to achieve comparable and unbiasedly itnerprtable results.

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    Tytuł oryginału: Distribution of nitric oxide synthase and neuropeptide Y neurones during the development of the hippocampal formation in the rat.
    Autorzy: Moryś Joanna M., Kowiański Przemysław, Moryś Janusz
    Źródło: Folia Morphol. 2002: 61 (4) s.221-232, il., tab., bibliogr. 55 poz.
    Sygnatura GBL: 301,720

    Hasła klasyfikacyjne GBL:
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Mitric oxide (NO) a short-liver radical, which modulates synaptic plasticity, neuronal oscillations and cerebral blood flow. NOS-containing neurones can be detected anatomically by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry or by NOS immunohistochemistry. Neuropeptide Y (NPY) is the most abundant peptide in the brain. NPY is connected with several vital functions, such as a feeding behaviour, sexual maturation, regulation of circadian rhythms, body temperature, blood pressure and neuroendocrine secretions. Neuropeptide Y also modulates anxiety-related disorders, limbic epileptic seizures as well as learning and memory processes. The study was performed on 45 Wistar rats of various ages (P0, P4, P7, P10, P14, P21, P30, P60, and P120; P - postnatal day). The free-floating sections were stained with standard immunohistochemistry methods. Thereafter the histological sections were studied using the confocal laser microscope equipped. For 3D reconstruction the image analysis program LaserSharp 2000v. 2.0 (bio-Rad, UK) was used. We found that in the newborn rat both NOS- and NPY-immunoreactivity was weak. It had been increasing gradually until the 7th day of postnatal life, after that until P14 it was maintained on the similar level, and then the number of immunolabelled cells deceased. The developmental changes concerned cell morphology as well - until the 10th day of life the immunoreactive cells were immature, with round or oval bodies and had only a few fibres. From Pf14 the cells' morphology became similar to that in adult.

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