Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL
Zapytanie:
KOWALCZEWSKI
Liczba odnalezionych rekordów:
3
Przejście do opcji zmiany formatu
|
Wyświetlenie wyników w wersji do druku
1/3
Tytuł oryginału:
Fibroblast-like synoviocytes from rheumatoid arthritis patients express functional IL-15 receptor complex: endogenous IL-15 in autocrine faschion enhances cell proliferation and expression of Bcl-xL and Bcl-2.
Autorzy:
Kurowska
Mariola,
Rudnicka
Weronika,
Kontny
Ewa,
Janicka
Iwona,
Chorazy
Magdalena,
Kowalczewski
Jacek,
Ziółkowska
Maria,
Ferrari-Lacraz
Sylvie,
Strom
Terry B.,
Maśliński
Włodzimierz
Źródło:
J. Immunol. 2002: 169 (4) s.1760-1767, il., bibliogr. 49 poz.
Sygnatura GBL:
300,982
Hasła klasyfikacyjne GBL:
reumatologia
Typ dokumentu:
praca kliniczna
praca opublikowana za granicą
tytuł obcojęzyczny
Wskaźnik treści:
ludzie
Streszczenie angielskie:
The hallmarks of rheumatoid arthritis (RA) are leukocytic infiltratin of the synovium and expansiveness of fibroblast-like synovicytes (FLS). The abnormal proliferation of FLS and their resistance to apoptosis is mediated, at least in part, bay present in RA joints proinflammatory cytokines and growth factors. Because IL-15 exerts properties of antiapoptotic and growth facators, and is produced by RA FLS, we hypothesized that IL-15 participates in RA FLA activation. To test this hypothesis, we first examined whether RA FLS express chains required for high affinity functional IL-15R. Indeed, RA FLS express IL-15Rŕ at mRNA and protein levels. Moreover, we confirmed the presence of IL-2Rá and common ç-chains. Interstingly, TNF-ŕ or IL-1á triggered significant elevation of IL-15Rŕ chanin at mRNA and protein levels. Next, we investigated the effects of exogenous or endogenous IL-15 on Bcl-2 andBcl-XL expression, FLS proliferation, and apoptosis. Exogenous IL-15 enhanced RA FLS proliferation and increased the level of mRNA-encoding Bcl-XL. To test the role of endogenous IL-15 in the activation of RA FLS, an IL-15 mutant/Fcç2a protein exerting properties of specific antagonist to the IL-15Rŕ chain was used. We found that blocking IL-15 biological activities using this protein substantially reduced endogenous expression of Bcl-2 and Bcl-XL, and RA FLA proliferation that was reflected by increased apoptosis. Thus, we havae demonstrated that a distinctive phenotype of RA FLS, i.e., persistent activation, proliferation, and resistance to apoptosis, is related to the autocrine activation of IL-15Rs by FLS-derived IL-15.
2/3
Tytuł oryginału:
High levels of osteoprotegerin and soluble receptor activator of nuclear factor kappaB ligand in serum of rheumatoid arthritis patients and their normalization after anti-tumor necrosis factor ŕ treatment.
Autorzy:
Ziółkowska
Maria,
Kurowska
Mariola,
Radzikowska
Anna,
Łuszczykiewicz
Grażyna,
Wiland
Piotr,
Dziewczopolski
Wojciech,
Filipowicz-Sosnowska
Anna,
Pazdur
Jacek,
Szechiński
Jacek,
Kowalczewski
Jacek,
Rell-Bakalarska
Maria,
Maśliński
Włodzimierz
Źródło:
Arthritis Rheum. 2002: 46 (7) s.1744-1753, il., tab., bibliogr. 32 poz.
Sygnatura GBL:
305,037
Hasła klasyfikacyjne GBL:
farmacja
reumatologia
Typ dokumentu:
tytuł obcojęzyczny
praca opublikowana za granicą
praca kliniczna
Wskaźnik treści:
ludzie
dorośli 19-44 r.ż.
dorośli 45-64 r.ż.
dorośli = 65 r.ż.
Streszczenie angielskie:
Objective. To test the hypotheses that 1) proinflammatory cytokines affect osteoprotegerin (OPG) and soluble receptor activator of nuclear factor kappaB ligand (sRANKL) production and therefore the OPG and sRANKL levels differ in rheumatoid arthritis (RA) patients in comparison with healthy individuals; and 2) anti-tumor necrosis factor ŕ (anti-TNFŕ) therapy influences OPG and sRNAKL levels. Methods. Sera were obtained from healthy individuals or RA patients receiving the combination of infliximab and methotrexate. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were isolated from RA patients. Fibroblast-like synoviocytes (FLS) were isolated from synovial tissue obtained at total knee replacement in RA patients. Supernatants from cells stimulated with cytokines were collected after culture in vitro. Concentrations of OPG and sRANKL were determined by enzymelinked immunosorbent assays. Results. A strong positive correlation between OPG concentration and age was observed in healthy individuals but not in RA patients. The OPG and sRANKL levels were higher in RA patients than in healthy controls. Cultured FLS spontaneously secreted much higher amounts of OPG than PBMCs or SFMCs. Proinflammatory cytokines enhanced OPG production. Anti-TNFŕ treatment resulted in the normalization of serum OPG and sRANKL levels in RA patients without influencing the OPG:sRANKL ratio. Conclusion. Although higher serum levels of OPG and sRANKL are present in RA patients than in healthy individuals, the ratio of OPG:sRANKL is similar. There is an age-dependent increase of OPG but not sRANKL levels in healthy subjects. Anti-TNFŕ treatment results in the normalization of elevated levels of OPG and sRANKL in RA patients.
3/3
Tytuł oryginału:
Działalność sekcji reumoortopedii Polskiego Towarzystwa Ortopedycznego i Traumatologicznego
Autorzy:
Kowalczewski
Jacek,
Michalak
Cezary,
Sak
Iwona
Źródło:
W: Ortopedia i traumatologia u progu nowego Millenium - Bydgoszcz, 2002 s.270 - 34 Zjazd Naukowy Polskiego Towarzystwa Ortopedycznego i Traumatologicznego pt. Ortopedia i traumatologia u progu nowego Millenium Bydgoszcz 18-21.09. 2002
Sygnatura GBL:
802,225
Hasła klasyfikacyjne GBL:
reumatologia
traumatologia i ortopedia
Typ dokumentu:
praca związana ze zjazdem
sprawozdanie
Temat korporatywny:
Polskie Towarzystwo Ortopedyczne i Traumatologiczne
stosując format:
z abstraktem i deskryptorami
z abstraktem
z deskryptorami
skrócony