Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: KITTUR
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Tytuł oryginału: Critical evaluation of radiolabeled lymphocytes to detect acute renal transplant rejection in a large animal model.
Autorzy: Petronis John D., Kittur Dilip S., Wilasrusmee Chumpon
Źródło: Med. Sci. Monitor 2002: 8 (12) s.BR515-BR520, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • immunologia
  • transplantologia
  • radiologia
  • nefrologia

    Typ dokumentu:
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • Świnie

    Streszczenie angielskie: Background: Renal transplant rejection cannot be diagnosed with certainly by non-invasive techniques. These techniques lack the specificity to differentiate rejection from other causes of renal dysfunction such as ATN and calcineuria toxicity. Since rejection involves lymphocytes, which the other course of dysfunction do not, radiolabelling lymphocytes is an attractive technique to diagnose rejection non-invasively. Material and methods: We report our experience with this technique in a pig model of renal transplantation. We studied two groups of pigs, one with renal autografts and the other with allografts. We optimized radiolabelling of lymphocytes and also the technology for detection of these lymphocytes. The uptake of the radiolabeled lymphocyte was compared between the two groups by surface detection and, at the end of the experiment, with scintigraphy of renal tissues. Results: Despite adequate labeling and viability of lymphocytes, only 1-2 p.c. of injected lymphocytes 'homed' to the renal grafts. Furthermore, although the detection technology was optimized, a poor signal to noise ratio interfered with the detection of the labeled lymphocytes. Due to these problems rejection could not be differentiated from ATN in this model. Conclusion: We conclude that increasing the specific activity of the lymphocytes and improving the signal to noise ratio will enhance the specificity and sensitivity of this technique and facilitate noninvasive diagnosis of rejection.

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    Tytuł oryginału: Immunostimulatory effect of Silybum Marianum (milk thistle) extract.
    Autorzy: Wilasrusmee Chumpon, Kittur Smita, Shah Gaurang, Siddiqui Josephine, Bruch David, Wilasrusmee Skuntala, Kittur Dilip S.
    Źródło: Med. Sci. Monitor 2002: 8 (11) s.BR439-BR443, il., tab., bibliogr. 21 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • immunologia
  • farmacja

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna

    Wskaźnik treści:
  • zwierzęta
  • myszy
  • in vitro

    Streszczenie angielskie: Background: Herbal products are increasingly used for their effects on the immune system. Milk Thistle, a commonly used herbal product is known to inhibit growth of certain tumors, although the mechanism of this effect remains unknown. Previously we have shown that Milk Thistle extracts stimulate neurons in culture. Since other drugs that affect the neuronal system also affect the immune system, we investigated the effects of Milk Thistle on the immune system. Material/Methods: Standardized Milk Thistle extract was studied in murine lymphocyte proliferation tests using Concanavalian A (ConA) as the mitogen for non-specific stimulation and mixed lymphocyte culture (MLC) as allospecific stimulation. Th1 and Th2 cytokine levels in MLC were assayed by two antibody capture ELISA technique. ALl tests were performed in triplicate and repeated twice. Reults: We found that Milk Thistle is immunostimulatory in vitro. In increased lymphocyte proliferation in both mitogen and MLC assays. These effects of MIlk Thistle were associated with an increase in interferon gamm, interleukin (IL-4) and IL-10 cytokines in the MLC (table). This immunostimulatory effect increased in response to icnreasing doses of Milk Thistle. Conclusions: Our study has uncovered a novel effect of milk thistle on the immune system. This immunostimulatory effect may be of benefit in increasing the immunity to infectious diseases.

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