Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL
Zapytanie:
KHL
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2
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Tytuł oryginału:
Digital image analysis system for the quantification of infiltrates and cell adhesion molecules in inflammatory cardiomyopathy.
Autorzy:
Noutsias
Michel,
Pauschinger
Matthias,
Ostermann
Karsten,
Escher
Felicitas,
Blohm
Jan-Henrik,
Schultheiss
Heinz-Peter,
Khl
Uwe
Źródło:
Med. Sci. Monitor 2002: 8 (5) s.MT59-MT71, il., tab., bibliogr. 27 poz.
Sygnatura GBL:
313,278
Hasła klasyfikacyjne GBL:
kardiologia
Typ dokumentu:
praca kliniczna
tytuł obcojęzyczny
Wskaźnik treści:
ludzie
dorośli 19-44 r.ż.
dorośli 45-64 r.ż.
płeć męska
płeć żeńska
Streszczenie angielskie:
We attempted to develop a digital image analysis (DIA) system for endomyocardial biopsies (EMBs) to reliably quantify a) biopsy quality, b) immunohistochemically-marked infiltrates, and c) cell adhesion molecules (CAMs) in relation to net heart area (HA) for the semi-automated diagnosis of inflammatory cardiomyopathy (InfCM). 140 EMBs from dilated cardiomyopathy (DCM) patients and 14 autopsy heart samples (controls) were immunostained for T-lymphocytes (CD2, CD3, CD4, CD8), á2-integrin + infiltrates (CD18, LFA-1, Mac-1) and CAMs (immunoglobulin superfamily: ICAM-1, HLA class I, HLA DR, VCAM-1, CD58; selectins: CD62E and CD62P; and the á1-integrin chain CD29). EMB quality was assessed visually on a three-point scale. Infiltrates were quantified visually (per hpf) and by DIA (per mm2 HA). CAM expression was evaluated semiquantitatively and bay DIA (area fraction [AF]: stained area relative to HA). DIA-evaluated HA correlated significantly with the visual assessment of EMB quality. The visual evaluation of both infiltrates and CAMs correlated significantly with the respective DIA-based quantification. DIA-quantified CAM-AF and infiltrates were discriminated bay the CAM clssification (CAMs+: n = 87; 62 p.c.) compared to controls. DIA-quantified cCAM immunoreacativity correlated significantly with the DIA-quantified counter-receptor + infiltrates. DIA evaluation of biopsy quality, infiltrates, and CAMs was devoid of inter- and intraobserver variability. The DIA system presented here enables standardized and observer-independent assessment of EMB quality and intramyocardial...
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Tytuł oryginału:
Phenotypic characterization of infilatrates in dilated cardiomyopathy - diagnostic significance of T-lymphocytes and macrophages in inflammatory cardiomyopathy.
Autorzy:
Noutsias
Michel,
Pauschinger
Matthias,
Schultheiss
Heinz-Peter,
Khl
Uwe
Źródło:
Med. Sci. Monitor 2002: 8 (7) s.CR478-CR487, il., tab., bibliogr. 55 poz.
Sygnatura GBL:
313,278
Hasła klasyfikacyjne GBL:
immunologia
kardiologia
Typ dokumentu:
tytuł obcojęzyczny
praca kliniczna
Wskaźnik treści:
ludzie
dorośli 19-44 r.ż.
dorośli 45-64 r.ż.
płeć męska
płeć żeńska
Streszczenie angielskie:
Background: Dilated cardiomyopathy (DCM) is linked to inflammatory cardiomyopathy (InfCM). To gain a profound insight into the underlying mechanisms, we phenotypically characterized pan leukocytes (CD18), naive T-lymphocytes (CD3, CD2, CD4, CD8), activated lympocytes (LFA-1, LFA-3, VLA-4, ICAM-1, CD69, CD45RO), macrophages (Mac-1, 27E10), B-lymphocytes (CD19) and NK-cells (CD57) in DCM and control hearts. Material/Methods: Biopsies from DCM patietns (n = 164, LVEF 40 p.c.) and specimens from non-cardiac death causes (controls; n = 17) were immunostained. Biopsies exceeding 2.0 CD3+ lymphocytes per high power field/hpf and/or 1.5 CD3+ lymphocytes/hpf with numerous foci and HLA class I/DR abundance were evaluated positive for InfCM. Results: InfCM+ biopsies (n = 102; 63 p.c.) demonstrated significantly increased infiltrates with respect to all studied phenotypes except for CD19 and CD57 when compared with both DCM biopsies negative for InfCM (n = 62) and the controls, whereas the latter two groups did not differ (Tukey-Kramer analysis). Virtually all phenotypes correlated with one another in multivariate analysis (except for B-lymphocytes and NK cells). Whereas HLA class I/DR abundance was present in 14 p.c. of the controls and 26 p.c. of the DCM biopsies not yielding InflCM, InfCM+ biopsies demonstrated significantly ( 0.001) higher frequencies of HLA abundance (76 p.c.). Conclusions: The inflammatory process in InfCM comprises T-lymphocytes and macrophages, whereas B-lymphocytes and NK-cells are not significantly increased. InfCM is associated with HLA induction...
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