Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: JANOWSKA-WIECZOREK
Liczba odnalezionych rekordów: 2



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Tytuł oryginału: Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells.
Autorzy: Baj-Krzyworzeka Monika, Majka Marcin, Pratico Domenico, Ratajczak Janina, Vilaire Gaston, Kijowski Jacek, Reca Ryan, Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
Źródło: Exp. Hematol. 2002: 30 (5) s.450-459, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 305,749

Hasła klasyfikacyjne GBL:
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: objective. peripheral blood plateled-derived microparticles (PMPs) circulate in blood and may interact directly with target cells affecting their various biological functions. methods. To investigate the effect of human PMPs on hematopoiesis, we first phenotyped them for expression of various surface molecules and subsequently studied various biological responses of normal stem/progenitor (CD34+) and more differentiated precursor cells as well as several leukemic cell lines to PMPs. Results. We found that, in addition to platelet-endothelium attachment receptors (CD41, CD61 and CD62), PMPs express G-protein-coupled seven transmembrane-span receptors such as CXCR4 and PAR-1; cytokine receptors including TNF-RI, TNF-RII, and CD95; and ligands such as CD40L and PF-4. Moreover, we found that several of these receptors could be transferred by PMPs to the membranes of normal as well as malignant cells and observed that PMPs: 1) chemoattract these cells, 2) increase their adhesion, proliferation, and survival, and 3) activate in these cells various intracellular signaling cascades including MAPK p42/44, PI-3K-AKT, and STAT proteins. The biological effects of PMPs were only partly reduced by heat inactivation or trypsin digest, indicating that, in addition to the protein components of PMPs, lipid components are also responsible for their biological activity. conclusion. We conclude that PMPs modulate biological functions of hematopoietic cells and postulate that they play an important but as yet not fully understood role in intracellular cross-talk in hematopoiesis...

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    Tytuł oryginału: Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets.
    Autorzy: Majka Marcin, Ratajczak Janina, Villaire Gaston, Kubiczek Krystyna, Marquez Leah A., Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
    Źródło: Exp. Hematol. 2002: 30 (7) s.751-760, il., tab., bibliogr. 51 poz.
    Sygnatura GBL: 305,749

    Hasła klasyfikacyjne GBL:
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: The development of megakaryocytes is regulated by thrombopoietin (TPO), which binds to the c-mpl receptor, and by several other cytokines such as interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), cilliary neurotropic factor (CNTF), and oncostatin (OSM), which bind to gp130 protein-coupled receptors. We attempted to identify signal transduction pathways activated by these factors in normal human megakaryocytes. Materials and methods: To better understand the role of these factors in normal human megakaryopoiesis we studied their effect on 1) purified human bone marrow-derived CD34+ cells, 2) human ŕIIbá3+ cells (shown by immunophenotypical and morphological criteria to be megakaryoblasts), which had been expanded ex vivo from CD34+ cells in chemically defined artificial serum, and 3) gel-filtered human peripheral blood platelets. Further, in an attempt to correlate the influence of these factors on cell proliferation and survival activation of signal transduction pathways, we evaluated their effect on the phosphorylation of MAPK p42/44 and activation of PI-3K-AKT and JAK-STAT proteins in these various cell types. Results: Using serum-free liquid cultures, we found that only TPO and IL-6 protected CD34+ cells and megakaryocytes from undergoing apoptosis (decrease in annexin-V binding, PARP cleavage, and activation of caspase-3). Moreover, only TPO when used alone and IL-6 only when used in combination with TPO, stimulated the growth of human colony-forming unitmegakarocytes (CFU-Meg) in semisolid serum-free medium...

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