Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: JAGUSZTYN-KRYNICKA
Liczba odnalezionych rekordów: 1



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku
1/1

Tytuł oryginału: Immunological characterization of the Campylobacter jejuni 72Dz/92 cjaD gene product and its fusion with B subunit of E. coli LT toxin.
Autorzy: Wyszyńska Agnieszka, Pawelec Dariusz P., Jagusztyn-Krynicka Elżbieta K.
Źródło: Acta Microbiol. Pol. 2002: 51 (4) s.313-326, il., tab., bibliogr. s. 324-326
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:
  • pediatria
  • genetyka
  • immunologia
  • mikrobiologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kliniczna

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Campylobacter jejuni 72Dz/92 cjaD gene, orthologue of C. jejuni NCTC 11168 cj0113, C. jejuni M275 ompl18 and C. jejuni ATCC 29428 omp18, has been cloned, sequenced and analysed from the viewpoint of its immunological attributes. Neither the 5' nor 3' fragment of the cjaD encodes protein capable of reacting with anti-Campylobacter antibodies. Several fusions of the cjaD with eltB, which encodes B subunit of the E. coli LT toxin, have been constructed. The hybrid proteins, which differ in respect to their cellular localization, retain the ability to react with GM1 and are recognized by the antibodies specific for both moieties of the proteins. The fusion protein equipped with signal sequence, reveals a stronger affinity to GM1 than ints equivalent which is unable to cross the inner membrane. Two recombinant plasmids (pUWM405 expressing both LTB and CjaD proteins and pUWM299 containing cjaD gene fused into 3' end of Escherichia coli eltB gene lacking signal sequence) were introduced into avirulent Salmonella enterica servoar Typhimurium strain where they are stably maintained.

    stosując format: