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Tytuł oryginału: Increased IL-10 production during spontaneous apoptosis of monocytes.
Autorzy: Bzowska Małgorzata, Guzik Krzysztof, Barczyk Katarzyna, Ernst Martin, Flad Hans-Dieter, Pryjma Janusz
Źródło: Eur. J. Immunol. 2002: 32 (7) s.2011-2020, il., bibliogr. 46 poz.
Sygnatura GBL: 312,318

Hasła klasyfikacyjne GBL:
  • immunologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Monocytes/macrophages undergo apoptosis and are in contact with apoptosis cells both in vitro and in vivo. The data show that monocytes undergoing spontaneous apoptosis in vitro change their cytokine production profile. We demonstrate that the lipopolysaccharide (LPS)-induced production of interleukin-10 (IL-1)) is up-regulated, while production of proinflammatory cytokines such as tumor necrosis factor-ŕ (TNF-ŕ) and interleukin-1á (IL-1á) is either not affected or reduced. These differences seen both at the protein and mRNA level directly correlate with the appearance of apoptotic cells in the culture. Flow cytometry analysis using double staining, surface with annexin V and intracellular with anti-IL-10, suggested that annexin V-negative monocytes are the predominant source of IL-10. Analysis of sorted populations of monocytes indicated that the increase in IL-10 synthesis appears to result from direct interactions between non-apoptotic and apoptotic cells at the time of stimulation. Also non-apoptotic, freshly isolated monocytes produced more IL-10 upon stimulation with LPS, Staphylococcus aureus or zymosan when apoptotic neutrophils were added to the culture. In contrast, monocyte-derived macrophages did not produce more IL-10 in the presence of apoptotic neutrophils. Finally, we found that the presence of apoptotic monocytes in the culture may influence specific immune responses. The data show that in the presence of annexin V-positive monotocyes CD-4 positive memmory T cells produce less IFN-ç upon stimulation with purified protein derivative of tuberculin...

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