Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: DULIŃSKA
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Tytuł oryginału: Comparative analysis of prostatic acid phosphatase and prostat-specific antigen mRNA levels in hyperplastic prostate stimulated with steroid hormones and growth factors.
Autorzy: Dulińska Joanna, Laidler Piotr, Łabędź Maria
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.357-368, il., tab., bibliogr. s. 366-368 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kliniczna

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) are the markers of human prostatic gland. However, it is still not completely understood if and how, steroid hormones and growth factors affect their expression and metabolism in the respect to the major pahtologies of the gland. Appropriate studies were carried out on histopathologically diagnosed benign prostatic hyperplasia - BPH (n - 42) using tissue slices and cells derived from them. They were incubated with steroid hormones: 5-ŕ-dihydrotestosterone (DHT), estradiol (E) and growth factors: epidermal growth factor (EGF), basic fibroblastic growth factor (bFGF) under culture conditiions for up to 24 hours. 32P-labelled specific oligonucleotide probes were used to analyze total RNA isolated from each sample for the presence of PAP and PSA mRNAs. DHT, E, bFGF, EGF or both DHT + bFGF and DHT + EGF increased PAP and PSA mRNA levels in a time- and dose-dependent manner. The highest and statistically significant increase (p 0.001) for PAP mRNA was observed when DHT + bFGF were present in the medium while for PSA mRNA if DHT + EGF were added to the medium.

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