Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: DOBRUCKI
Liczba odnalezionych rekordów: 3



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Tytuł oryginału: Increased nitric oxide bioavailability in endothelial cells contributes to the pleiotropic effect of cerivastatin.
Autorzy: Kalinowski Leszek, Dobrucki Lawrence W., Brovkovych Viktor, Maliński Tadeusz
Źródło: Circulation 2002: 105 (8) s.933-938, il., bibliogr. 17 poz.
Sygnatura GBL: 311,033

Hasła klasyfikacyjne GBL:
  • farmacja
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Although statins preserve endothelial function by reducing serum cholesterol levels, it has been suggested they may also stimulate nitric oxide (NO) synthase in endothelium with concurrent increase in superoxide (O2-) generation, leading to impairment of NO activity. Therefore, measurements of biologically active NO and O2 - in endothelium after exposure to the HMG-CoA reductase inhibitor cerivastatin were undertaken to evaluate its potential effect on NO biological activity. Methods and Results - Highly sensitive electrochemical NO and O2 - microsensors were placed near the surface of a single human umbilical vein endothelial cell, and the kinetics of NO and O2 - release were recorded in vitro. Cerivastatin demonstrated a time-dependent effect on NO release in endothelial cels. The initial release (approximately the first 3 minutes) was concentration-dependent (0.01 to 10 ćmol/L) and was similar to that observed for typical NO synthase agonists calcium ionophore or acetylcholine. Cerivastatin stimulated NO release at a favorable rate and scavenged O2-, which led to the preservation of the active concentration of NO. The sustained effect (after ÷ 6 hours) of cerivastatin on endothelium was associated with an ÷ 35 p.c. increase in NO release as compared with the initial effect. In contrast to the initial effect, the sustained effect of cerivastatin was shown at concenrations ÷ 100-fold lower and was dependent on inhibition of endothelial HMG-CoA reductase. Conclusions - These data provide direct evidence to prove that in the presence of cerivastatin, the NOS system in endothelium operates with high efficiency toward increasing NO activity by activation of NO release and by concurrent inactivation of O2-.


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    Tytuł oryginału: Mikroemulsje jako potencjalne nośniki substancji leczniczych w preparatach farmaceutycznych.
    Tytuł angielski: Microemulsions as potential carriers of drugs in pharmaceutical preparations.
    Autorzy: Radomska Anna, Dobrucki Roman
    Źródło: Farm. Pol. 2002: 58 (5) s.218-225, sum. - 18 Zjazd Naukowy Polskiego Towarzystwa Farmaceutycznego Poznań 21.09. 2001
    Sygnatura GBL: 310,851

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca związana ze zjazdem

    Streszczenie angielskie: In the past few years microemulsions have been a subject of extensive research work, because of vast possibilities they offer a variety of technological applications. Microemulsions are potential drug carrier system for oral, topical and parenteral administration. Their properties such as thermodynamic stability, improved drug solubilization and bioavailability make them very attractive drug carrier systems. The review is started with a brief introduction and followed by the definition of microemulsions, their formation characteristic and the effect of additives. An overview of the various microemulsions application and the current state of knowledge in the field is presented in this review.


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    Tytuł oryginału: Cerivastatin potentiates nitric oxide release and enos expression through inhibition of isoprenoids synthesis.
    Autorzy: Kalinowski L., Dobrucki I. T., Maliński T.
    Źródło: J. Physiol. Pharmacol. 2002: 53 (4) p. 1 s.585-595, il., bibliogr. 32 poz.
    Sygnatura GBL: 302,092

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Endothelium dysfunction, which is often defined as a decrease in NO bioavailability, is one of the earliest manifestations of endothelium-impaired finction disorders, including atherosclerosis. Although improvement in NO bioavailability has been attributed to the lowering of serum cholesterol levels, recent studies suggest that HMG-CoA reductase inhibitors, statins, may have direct effects on NO bioavailability by little known mechanisms that are independent of serum cholesterol levels. The long-term effect of cerivastatin on NO release from endothelial cells was determined by using highly sensitive electrochemical microsensors and was correlated with endothelial NO synthase (eNOS) levels. To explore whether changes in isoprenoid synthesis affect NO bioavailability and eNOS expression, human endothelial cells were treated with cerivastatin, L-mevalonate (MVA; 1.5 mmol/L), geranylgeranylpyrophosphate (GGPP; 1mg/mL) and farnesylpyrophosphate (FPP; 1mg/mL). Cerivastatin increased spontaneous (by 53 p.c. ń 6) and an eNOS-stimulated NO release (by 41 ń 6 p.c. for calcium ionophore and by 47 ń 5 p.c. acetylcholine) as well as eNOS expression (by 118 ń 6 p.c.) in the same concentration-range. Cerivastatin-dependent increase in both NO release and eNOS expression was revealed after ~4h of exposure reaching the maximum after ~10 h. Co treatment with MVA or GGPP, but not FPP or LDL, reversed the effects of cerivastatin. These findings indicate that the long-term effect of cerivastatin resulting in enhanced ...

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