Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: CIERNIEWSKI
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Tytuł oryginału: GATA-1 binding to the ŕv promoter negatively regulates expression of the integrin ŕv subunit in human leukemic K562 cells.
Autorzy: Czyż Małgorzata, Stasiak Marta, Boncela Joanna, Cierniewski Czesław S.
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.19-28, il., bibliogr. 30 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:
  • genetyka
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions - 194 and - 172 of the ŕv promoter region and are directly involved in the regulation of transcriptional activity of teh ŕv gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates ŕv expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the ŕv gene promoter. DNase I footprinting analysis revealed that region - 413 to - 408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced ŕv mRNA and ŕv protein onn the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated wtih increased leveld of ŕv. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the ŕv gene promoter and negatively regulates ŕv gene expression.

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    Tytuł oryginału: Natriuretic peptides reduce plasminogen activator inhibitor-1 expression in human endothelial cells.
    Autorzy: Pawłowska Zofia, Jerczyńska Hanna, Szemraj Janusz, Barańska Patrycja, Świątkowska Maria, Cierniewski Czesław S.
    Źródło: Cell. Mol. Biol. Lett. 2002: 7 (4) s.1153-1157, il., bibliogr. 10 poz.
    Sygnatura GBL: 306,513

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Plasma concentrations of natriuretic peptides increase in some pathological conditions, but very little is known about the effect of these vasodilator peptides on the regulation of the blood coagulation system. The fundamental role in the regulation of fibrinolysis is played by plasminogen activator inhibitor type 1 (PAI-1). Recent studies demonstrate that natriuretic peptides can modulate PAI-1 expression in bovine aortic smooth muscle cells and rat aortic endothelial cells. In this report, we tested the effect of natriuretic peptides on PAI-1 expression in the human endothelial cell line (EA.hy 926). For this purpose, we treated the cell cultures with ANP, BNP and CNP, and modulation of PAI-1 synthesis was evaluated. We compared the effect of natiuretic peptides on synthesis and release of PAI-1 in unstimulated cells, and after activation with tumour necrosis factor ŕ (TNFŕ). Natriuretic peptides abolished TNFŕ- induced upregulation of PAI-1 expression at both the PAI-1 mRNA and the antigen levels. The inhibitory efficiency was higher in the case of CNP when compared to that produced by ANP and BNP, particularly when TNFŕ-stimulated cells were used. We observed an inhibition of stimulatory effect of TNFŕ on PAI-1 expression also at the level of the PAI-1 promoter in cells transfected with a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was markedly inhibited by C-type natriuretic peptide, already at a very low (0.001 ćM) concentration of the peptide.

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