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Tytuł oryginału: Melatonin effects on megakaryocyte membrane patch-clamp outward K+ current.
Autorzy: Di Bella Luigi, Bruschi Carla, Gualano Luciano
Źródło: Med. Sci. Monitor 2002: 8 (12) s.BR527-BR531, il., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • psy
  • in vitro

    Streszczenie angielskie: Background: This study was carried out to evaluate the influence of melatonin concentration on rat bone marrow megakaryocyte outward K+ current and its implications with regard to platelet production. It is the Author's view that the greatly extended development of megakaryocyte membrane, together with its ion channels, makes the choice of this topic particularly pertinent. Material and methods: megakaryocytes from fresh Wistar rat bone marrow were clampaged with patch-clamp technique and the examination of membrane outward current was performed when melatonin dissolved in external or internal standard solution was used. Results: On the basis of this study, melatonin does reduce outward K+ current intensity, the more the higher the melatonin concentration. In quantitative terms, whereas no change is noticed when dissolved melatonin concentration in external standard solution is smaller than 25 ćM, at 50 ćM only a delayed outward current decrease appears. The effect on the outward current intensity is reversible at least until melatonin concentration reaches 500 ćM; when melatonin concentration is higher than 1000 ćM the effects is demonstrably irreversible. The presence on the megakaryocyte membrane of internal standard melatonin solution does reduce the outward current even more sharply. Conclusions: There seems to be a positive correlation beween cationic pump and megakaryocyte intimate processes of platelet production.

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