Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: BHATTACHARYYA
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Tytuł oryginału: Serodiagnostic and immunoprophylactic potential of a 78kDa protein of Leishmania donovani of Indian origin.
Autorzy: Mukherjee Mandira, Bhattacharyya Anindita, Duttagupta Swadesh
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR117-BR122, il., tab., bibliogr. 35 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy
  • in vitro

    Streszczenie angielskie: The serodiagnosis of visceral leishmaniasis due to Leishmania donovani using crude parasite antigen is complicated in many endemic areas by cross-reactions with serum from humans infected with other protozoan diseases. The search for pure antigens avoiding such cross-reactions is in progress. Developing a vaccine against cutaneous leishmaniasis has been much more successful than against visceral leishmaniasis. Immunoprophylactic studies using various combinations of antigens and adjuvants are also in progress, and several strategies are in use, with varying degrees of success. Promastigotes of Leishmania donovani were used. The 78kDa protein was purified by a monoclonal antibody tagged GNBr sepharose CL-4B column. The presence of the protein in both stages of the parasite and in kala-azar patient serum was analyzed by western blotting. ELISA was used for serodiagnosis and isotype analysis of antibodies produced in immunized mice. Immunoprophylactic studies were carried out based on in vitro transformation of amastigotes to promastigotes. The 78 kDa membrane protein, present in both amastigote and promastigote forms of the parasite, was purified to homogeneity. The protein was found to have serodiagnostic potential to detect kala-azar. BALB/c mice immunized with 78kDa protein revealed reduction in spleen parasitemia. Isotype profiles of antibodies produced by immunized mice showed increased production of IgG2a and decreased IgG levels. Our results demonstrated, for the first time, the serodiagnostic and immunoprophylactic use of a pure membrane protein isolated from Leishmania donovani of Indian origin...

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    Tytuł oryginału: Effect of thyroxine on some digestive enzymes of the adult male toad, Bufo melanostictus.
    Autorzy: Bhattacharyya Sanat K., Chaki Korak K., Misra Kamales K.
    Źródło: Folia Biol. 2002: 50 (1/2) s.83-90, tab., bibliogr. s. 89-90
    Sygnatura GBL: 302,991

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • gastroenterologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • Żaby
  • płeć męska

    Streszczenie angielskie: Thyroxine is known to play an important role during the developmental process of amphibians. The present work is designed on the hypothesis that a functional relationship exists between the thyroid gland and digestive physiology in the adult toad. Three doese of thyroxine (25 ćg/100 g; 50 ćg/100 g; 100 ćg/100 ćg body weight) were orally administered daily to the adult male toad, Bufo melanostictus and changes in -amylase, pepsin, trypsin, and esterase activities of the different zones of the digestive tract, pancreas and liver were observed. The observations were made on 7, 15, and 30 days of thyroxine treatment. The result shows that the dose of 25 ćg increases the enzyme activities in the gastrointesinal tract, liver and pancreas of 7 days treatment. However, prolonged treatment with all the doses shows little effect on these enzymes. The pattern of changes in the enzymatic activities in the digestive tract of the adult male toads show more advanced compartmentalizaiton than that of fishes. It is also found that site-specific enzyme production is not pronounced in this amphibious vertebrate. It is proposed that a positive functional relationship between thyroxine and digestive enzymes exists in the adult male toad.

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