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Zapytanie: ŻAKOVSKA
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Tytuł oryginału: Positive findings of Borrelia burgdorferi in Culex (Culex) pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method.
Autorzy: Żakovska Alena, Nejedla Petra, Holikova Alena, Dendis Milos
Źródło: Ann. Agric. Environ. Med. 2002: 9 (2) s.257-259, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: After first finding Borrelia in the midgut of imago mosquitoes, we concentrated on the presence of Borrelia in mosquitoe development stages - larvae of the third or fourth instar. In the summer season in the years of 2000 - 2001 a total of 439 Culex (Culex) pipens pipens larvae were collected from a barrel of rainwater in the Obrany holiday area of Brno city (East Moravia, Czech Republic). The larvae midgut was observed under dark-field microscopy. Ten DFM positive samples (2.28 p.c.) were further analysed using the single-tube nested PCR method for the presence of flagellum DNA sequence specific for Borrelia burgdorferi sensu lato, of which 5 were positive. Borrelian positivity of Culex (C.) pipens pipens larvae was 1.14 p.c. One spirochete isolated strain in BSK-H medium was obtained. PCR detection for borrelian DNA of the isolated strain was negative. From these results we can conclude that a low percentage Borreliae can be also found in mosquitoe larvae and are likely to survive into imago stage.

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