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Tytuł oryginału: N-methyl-d-aspartate receptor-mediated processing of á-amyloid precursor protein in rat hippocampal slices: in vitro - superfusion study.
Autorzy: Gordon-Krajcer Wanda, Salińska Elżbieta, Łazarewicz Jerzy W.
Źródło: Folia Neuropathol. 2002: 40 (1) s.13-17, il., bibliogr. 26 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć męska
  • in vitro

    Streszczenie angielskie: Abnormal proteolytic degradation of the á amyloid precursor protein (á-APP) may result in accumulation of potentially neurotix á amyloid (áA). The role of various receptors in the regulation of á-APP processing has been suggested. This study aimed to determine how NMDA receptors and Caý+ ions regulate proteolysis of á-APP in rat hippocampus in vitro. Adult rat hippocampal slices were superfused with NMDA-containing media, and immunoreactivity of soluble á-APP derivatives was detected in dialysates. Application of 100 ćM and 250 ćM NMDA for 20 min in Caý+-containing medium induced dose-dependent release of amino-terminal á-APP derivatives, and a fragment of Aá sequence, whereas carboxy-terminal fragments of á-APP were only slighty detected. This indicates activation of á-APP processing, and release of its soluble cleavage products. This effect was inhibited by NMDA receptor antagonist 1 ćM MK-801 and 100 ćM CPP in Caý+-free medium, thus indicating that NMDA receptors and calcium ions mediate proteolytic non-amyloidogenic degradation of the á-APP.

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