Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: ŁĘKOWSKA-KOCHANIAK
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Tytuł oryginału: Detection of Escherichia coli O157:H7 in raw meat by immunomagnetic separation and multiplex PCR.
Autorzy: Łękowska-Kochaniak Aneta, Czajkowska Danuta, Popowski Janusz
Źródło: Acta Microbiol. Pol. 2002: 51 (4) s.327-337, il., tab., bibliogr. s. 336-337
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:
  • genetyka
  • immunologia
  • mikrobiologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna

    Streszczenie angielskie: The aim of this research was to elaborate fast and sensitive method of detection of E. coli O157:H7 in food samples. Raw ground meat obtained from retail was artificially inoculated with low numbers of E. coli O157:H7. 18 h enrichment culture allowed pathogenic bacteria to multiply to the levels detectable in multiplex PCR. Immunomagnetic separation with magnetic beads coated with an antibody against E. coli O157:H7 were used to concentrate target bacteria and to separate PCR inhibitors. A portion of the bacterial suspension was used in a multiplex PCR to amplify eae (attaching and effacing) gene, stx (shiga toxin) genes and 90 kbp plasmid. The sensitivity of E. coli O157:H7 detection method was shown to be 1 cfu per 25 g of food sample. The total analysis can be completed within 24 h, whilst traditional methods involves enrichment, direct plating and confirmation tests with entire time at least 3 days.

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