Wynik wyszukiwania

tytuł obcojęzyczny

Wskaźnik treści:

historia XIX wieku

2/2523

Tytuł oryginału: Are there any disturbances in vestibular organ of children and young adults with type I diabetes?
Autorzy: Gawron W., Pośpiech L., Orendorz-Frączkowska K., Noczyńska A.
Źródło: Diabetologia 2002: 45 (5) s.728-734, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 312,048

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Aims/hypothesis. The aim of the work was to evaluate the vestibular organ condition in children and young adults suffering from Type I (insulin-dependent) diabetes mellitus. Methods. The group examined consisted of 95 children and young adults aged from 6 to 28 years with Type I diabetes diagnosed. The diabetic group was divided into subgroups according to duration of the disease, compensation of the disease, and presence and character of hypoglycaemic incidents, and presence of diabetic complications. The control group consisted of 44 healthy children and young adults aged 6 to 28 years. After collecting detailed medical history in each case an electronystagmographic test was performed using the computed two-canal electronystagmographer. Results. Within the diabetic group 6 patients complained about vertigo and balance disorders. Spontaneous nystagmus occurred in 10 cases, positional one in 21 cases. Impaired optokinesis occurred in 36 cases and impaired eye tracking test in 33 cases. In caloric tests there was partial canal paresis in 4 cases and directional preponderance in 7 cases. Conclusion/interpretation. Metabolic disturbances present in Type I diabetes cause disturbances in different parts of vestibular organ but mostly in its central part. Comparing disturbances in the vestibular organ with clinical and biochemical parameters characterising diabetes, the range of vestibular organ impairment in diabetes mellitus type 1 seems to depend mainly on the presence and character of hypoglycaemic incidents and the duration of the disease and to some extent on the compensation of diabetes.

3/2523

Tytuł oryginału: Antiaggregatory activity of hypoglycaemic sulphonylureas.
Autorzy: Siluk D., Kaliszan R., Haber P., Petrusewicz J., Brzozowski Z., Sut G.
Źródło: Diabetologia 2002: 45 (7) s.1034-1037, il., tab., bibliogr. 11 poz.
Sygnatura GBL: 312,048

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Aims/hypothesis. Vascular complications observed in diabetes are often related to altered platelet functions. The most widely used hypoglycaemic drugs for treating Type II (non-insulin-dependent) diabetes mellitus are sulphonylurea derivatives. The purposes of this study were to evaluate the inhibitory effects of hypoglycaemic agents on platelet aggregation, to measure their lipophilicity and identify their structural parameters which assess their antiaggregatory activity. Methods. An antiaggregatory test in vitro was carried out for 13 sulphonylurea derivatives. Aggregation of platelets, incubated with the agents at concentrations varying from 7.5 to 480 ćmol/l, was induced by 10 ćmol/l ADP. Drug lipophilicity parameter, log kw, was measured by gradient HPLC and the agents were subjected to molecular modelling. Results. The most pronounced inhibition of platelet aggregation was by glimepiride, gliclazide, gliquidone, glibenclamide and compound 2A. The IC25 values were 15.9, 18.6, 20.4, 28.5 and 34.7 ćmol/l, respectively. Quantitative structure-activity relationships indicate that antiaggregatory activity is mainly affected by electronic and not by lipophilic properties of the agents. Conclusion/interpretation. Glimepiride appeared to be a more potent ADP-induced platelet aggregation inhibitor in vitro than gliclazide. Antiaggregatory activity was shown for gliquidone and confirmed for glibenclamide. The QSAR analysis supports the hypothesis of a free radical mechanism of action of sulphonylurea derivatives previously suggested for gliclazide.

4/2523

Tytuł oryginału: History and present situation of Paediatric ENT Surgery in Poland and in other Central East European countries.
Autorzy: Chmielik Mieczysław, Bielicka Anna, Ranocha Cezary, Chmielik Lechosław
Źródło: New Med. 2002: 5 (2) s.46-48, tab., bibliogr. 5 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: One of the first paediatric ENT centres in the world was the research group managed by Jan Danielewicz in Warsaw. It was organised on the basis of progress clinics and paediatric ENT wards, and a programme of specialisation was established (1, 2). Almost simultaneously, in the sixties of the twentieth century, centres for paediatric ENT were established in Prague, Berne, Bratislava, Budapest, Bucharest and Sophia. In the nineteen-seventies these were in touch with the centre for paediatric ENT in Italy, which was the pioneer in this speciality in West Europe. The effect of this contact was the creation of the European Federation of Oto-Rhino-Laryngology Societies, and the International Federation of Oto-Rhino-Laryngological Societies (IFOS), and further dynamic progress in this specialisation.

5/2523

Tytuł oryginału: Tracheotomy in children - indications and complications.
Autorzy: Zawadzka-Głos Lidia, Chmielik Mieczysław
Źródło: New Med. 2002: 5 (2) s.49-52, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Tracheostomy is a life-saving procedure when pertbrmecl with an appropriate indication and surgical technique. There is a limited indication for tracheostomy procedures in paediatrie surgery. It is rarely applied to the paediatric patient because they can be kept intuhated for a longer period compared to adults. Indications for tracheostomy are prolonged intubation, subglotie stenosis, general body trauma, tracheomalasia, cervical tumour pressing the trachea and larynx, congenital diseases of the larynx, burn injuries of the trachea, and foreign body aspiration. In the last decade the number of cases with tracheotomy increased due to the development of new intensive care units, the use of mechanical ventilation, and the increasing number of patients needing prolonged ventilation support. Tracheotomy in children lias been associated with significant operative and post-operative complications. When long-term tracheotomy is needed, parent education, together with special equipment and environment may allow the return of the tracheotomised child to home in secure conditions.

6/2523

Tytuł oryginału: Nasal and sinus diseases in children - surgical treatment.
Autorzy: Chmielik Mieczysław, Brożek Eliza
Źródło: New Med. 2002: 5 (2) s.53-54, bibliogr. 10 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Plastic surgery of the lateral nasal wall in children has been performed in Warsaw Paediatric ENT Clinic since 1995. There have been 121 operations. They consisted of conservative reconstruction of congenital or acquired defects in this area. In most eases a decrease in the number of upper airways infections and permanent improvement of nasal patency was attained. None of the cases lias shown any inhibitory influence from these procedures on craniofacial development. The principles of accomplishing conservative plastic surgery of the nasal mucous membrane are defined.

7/2523

Tytuł oryginału: Laryngeal haemangioma in children.
Autorzy: Chmielik Mieczysław, Zając Beata
Źródło: New Med. 2002: 5 (2) s.55-57, bibliogr. 22 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Haemangiomas and vascular malformations located in the larynx are rare hut potentially life-threatening conditions, because they compromise the airway. The authors present the clinical symptoms and method of diagnostic evaluation of these lesions. Various techniques for the treatment oflaryngeal haemangioma are discussed, but no single approach has universal acceptance.

8/2523

Tytuł oryginału: Assessment of hearing in children in postoperative treatment of choanal atresia.
Autorzy: Zawadzka-Głos Lidia, Bielicka Anna, Chmielik Mieczysław
Źródło: New Med. 2002: 5 (2) s.58-60, tab., bibliogr. 12 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Choanal atresia is a congenital abnormality often coexisting with other developmental abnormalities. The treatment of choice his condition is surgical restoration of choanae patency using a transnasal or transpalatal operative approach. After ;ical treatment, separators are fixed for several weeks in the posterior choanae and nasopharynx. Surgical transpalatal rvention and extended retention of separators in the posterior choanae and nasopharynx may cause auditive tube unction and otitis media with effusion. The aim of this work was the estimation of the occurence of otitis media with sion in children under post-operative treatment after choanal atresia depending on the surgical approach applied. immary of the period 1959-2002 in the Department of Paediatric Otorhinolaryngology in Warsaw shows that 175 operations s performed because of choanal atresia. A group of 11 children operated on choanal atresia between 1996-2002 was nined. Acoustic impedance audiometry was performed in all children. Bilateral choanal atresia was recognised in 4 children, unilateral choanal atresia in 7 children. A tympanomctry curve type B was obtained in 4 children, but a tympanometry curve type A was found in 7. In the examined group we did not find a dependence between the type of operative approach in surgical treatment of choanal atresia and otitis media with effusion occurrence.

9/2523

Tytuł oryginału: Tonsil operations in children - indications and contradictions.
Autorzy: Chmielik Mieczysław, Bielicka Anna, Brożek Eliza
Źródło: New Med. 2002: 5 (2) s.61-63, bibliogr. 10 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Adenoidectomy, tonsillectomy and tonsillotomy were and still are the most common surgical procedures performed in Paediatric Otorhinolaryngology. The aim of this study is to justify the use of these methods in particular cases, and also specifying the complications that might occur in the postoperative period. The indications for the procedures are divided into definite and relative, while the complications are separated into two groups, early and late. The information given in the text is based on the experience of the Paediatric ENT Clinic of the Medical University of Warsaw, and the available literature.

10/2523

Tytuł oryginału: Laryngeal papillomatosis in children and their surgical treatment.
Autorzy: Zawadzka-Głos Lidia, Chmielik Mieczysław, Bielicka Anna
Źródło: New Med. 2002: 5 (2) s.64-66, bibliogr. 17 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Laryngeal papillomas in children constitute a disease of viral aetiology caused by Human Papilloma Virus. Even though the changes arc histologically benign, their exophytic growth may lead to a critical airway obstruction and the child's suffocation. Due to the tendency to recur, lack of causal treatment, and risk of malignancy the optimal method of treating this condition is still being sought. The study presents methods applied nowadays in the treatment of laryngeal papillomas; especially, it concentrates on a classical papillotomy and its main advantage: the diminished risk of scar formation leading to laryngeal stenosis and vocal fold injury with speech disorders.

11/2523

Tytuł oryginału: Vertigo and dizziness in children - diagnostic problems.
Autorzy: Chmielik Jolanta, Chmielik Mieczysław, Zając Beata, Gabryszewska Anna, Szapłyko Waldemar
Źródło: New Med. 2002: 5 (2) s.67-70, il., bibliogr. 11 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The purpose of the study was to review clinical presentation, diagnostic methods, and conditions causing vertigo and dizziness in children. The authors report a retrospective review of 70 children diagnosed in the ENT Department or Outpatient Clinic in the Department of Paediatric Neurology. The differential diagnosis of dizziness and vertigo in children requires a multidisciplinary approach. Close co-operation between the otolaryngologist, paediatrician, neurologist and ophtalmologist allows the best chance of accurate diagnosis. From an analysis of the results, a diagnostic approach to the dizzy child is suggested, and the most frequent conditions causing vertigo in children are discussed.

12/2523

Tytuł oryginału: Ventilating tube insertion in the treatment of otitis media with effusion in children.
Autorzy: Dębska Małgorzata, Bielicka Anna, Chmielik Mieczysław
Źródło: New Med. 2002: 5 (2) s.71-72, bibliogr. 17 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The authors present methods of surgical treatment of otitis media with effusion in children. The advantages and complications of ventilating tube insertion are given particullar attention.

13/2523

Tytuł oryginału: Clinical aspects of ethmoiditis.
Autorzy: Chmielik Mieczysław, Gabryszewska Anna
Źródło: New Med. 2002: 5 (2) s.73-75, bibliogr. 12 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Ethmoiditis is a condition which any GP may meet in his practice. In this paper we describe the pathomechanisms which lea( to acute and prolonged sinusitis in children, the diagnostics, and prognosis. Particular attention is paid to complications which may be tragic in children. We also consider possible treatments and management when complications occur.

14/2523

Tytuł oryginału: Frontometaphyseal dysplasia in a 3 5/12 year-old girl and in the "unaffected mother".
Autorzy: Marik Ivo, Kozlowski K.
Źródło: New Med. 2002: 5 (2) s.76-79, il., bibliogr. 15 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We report a girl with fromtometaphyseal dysplasia in whom the first phenotypic abnormality noted at the age of 3 months was metopic suture torus. At the end of the first year of life knock knee deformity and torsion of the legs was noted and this progressed in spite of orthotic treatment. The diagnosis of frontometaphyseal dysplasia was established at the age of 3 years 5 months. Family studies revealed that the mother, supposed to be normal was also affected. Previously unreported clinical manifestations and radiographic findings are documented to further delineate and expand the spectrum of the disorder.

15/2523

Tytuł oryginału: A new, distinctive from of spondylo-epi-metaphyseal dysplasia.
Autorzy: Beighton P., Kozlowski K.
Źródło: New Med. 2002: 5 (2) s.80-82, il., bibliogr. 5 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Spondylo-epi-metaphyseal dysplasias are a group of complex disorders, most of which defy classification. We report a patient with distinctive radiographic features who presents a previously undescribed form of spondylo-epi-metaphyseal dysplasia.

16/2523

Tytuł oryginału: Hallucinations, illusions, and other visual disturbances in neurology.
Autorzy: Brzecki Andrzej, Kobel-Buys Krystyna, Buys Guido
Źródło: New Med. 2002: 5 (2) s.83-88, il., bibliogr. 57 poz.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The authors describe the visual pathway and especially the posterior part of the optic visual radiation and visual representation in the cuncus of the occipital cortex. Almost the entire visual tract is supplied by the posterior cerebral artery, except for central vision representation, which is supplied by a branch of the middle cerebral artery. Therefore, damage to the visual pathway of one hemisphere may cause hetcronymous hemianopsia or quadrantanopsia and, less frequently, visual hallucinations and illusions. Hallucinations and illusions may also occur - exempli gratia - in infectious encephalopathies, poisonings, sleep disorders and psychoses. In particular, stimulation of the temporal lobe by toxins or drugs having dopamincrgic, semtoninergic or adrenergic actions (frequently true or ,,false" neurotransmitters) may also cause them. Examples of paintings done by artist under the influence of some hallucinogens has been presented. Prosopgnosia and prosopagnosia have been discussed. Prosopagnosia may be caused by an injury the right hemisphere. Anosognosia of the left part of the body and the left visual field have to been also discussed. A case study of a patient with visual hallucinations and illusions of his own enormous extremities in the course of thrombosis of the posterior cerebral artery has been mentioned. Also, in connection with this theme, we discussed anosognosia of the left part of the body and left visual field spatial neglect, cortical blindness etc. and we mentioned border zone infarcts as a new problem of vascular diseases in elderly patients. ...

17/2523

Tytuł oryginału: Analihz pihdgotovki sihmejjnikh lihkarihv v dejakikh krajinakh svihtu ta problemi vprovadzhennja sihmejjnoji medicini v Ukrajinih.
Tytuł angielski: Comparative characteristic of family medicine in some countries and the possibility of introduction them in Ukraine.
Autorzy: Chopejj Ih. V.
Źródło: New Med. 2002: 5 (1) s.3-9, bibliogr. 11 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

organizacja ochrony zdrowia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In the paper presented the various models of family medicine. There are done the conclusion about the adequate model for Ukraine in the transitive economic period.

18/2523

Tytuł oryginału: Dosvihd nadannja pervinnoji mediko-sanihtarnoji dopomogi khvorim na khronihchnih obstruktivnih zakhvorjuvannja legen, jajkih poterpihli vnasljidok ekologihchnoji katastrofi - povenih.
Tytuł angielski: Experience of the primary medical and sanitary help render to patients with chronic obstructive pulmonary diseases, who were suffering from ecological disaster - flood.
Autorzy: Rogach Ih. M., Lemko Ih. S., Melega-Zajac K. P., Melega O. O., Lemko O. Ih., Brich V. D., Ljakh V. V., Kihrejj K. Ja, Gabor M. L., Kazankevich V. P.
Źródło: New Med. 2002: 5 (1) s.10-12, bibliogr. 6 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The peculiarities of chronic obstructive pulmonary diseases in patients who were suffering from flood were studied in dependence influence's nature of the ecological disaster factors. The ways and means of medical and sanitary help efficiency's increase were shown in account of nosology, disease's severity, nature of flood factors influence, period, which has passed after flood.

19/2523

Tytuł oryginału: Kontrol za efektivnihstju trivaloji antigihpertenzivnoji terapihji enalaprilom v praktichnihjj robotih sihmejjnogo lihkarja.
Tytuł angielski: Checking the effcacy of prolonged antihypertention therapy with enalapril in practical woru of a family doctor.
Autorzy: Nejjko Je. M., Orinchak M. A., Seredjuk N. M., Gorban Je. M., Vakaljuk Ih. P., Aleksandruk D. P., Jacishin R. Ih., Vihrstjuk N. G., Seredjuk V. N., Glushko N. L., Artemenko N. R., Lihbrik O. Ja., Cherkashina O. Je.
Źródło: New Med. 2002: 5 (1) s.13-15, bibliogr. 6 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: 137 patients in the middle and older age with arterial hypertension (AH) of the P and Sh stage were observed. The speading of AH in different regions of Precarpathia in dependance from climatical - geografical and socioeconomical conditions was studied. It was stated that the indexes of the daily monitoring of blood preassure are informational prognostical criterial of AH running and also are the criteria of the effectivness of the antihypertensive therapy. The effectiveness of the long time use of the angiotensin-converting enzyme inhibitor of enalapri in different age groups of the patients was proved. The study of psychological condition of the patient with the help to the questioneer may serve as the criteria of valuing the effectiveness of healing and social rehabilitation of the AH patients. The recommendation for the family doctors concerning healing and the prevention of the complicates of the AH on the base of the researth results were worked out.

20/2523

Tytuł oryginału: Suchasnih programi monihtoringu zakhvorjuvan' organihv travlennja u prakticih lihkarja sihmejjnoji medicini.
Tytuł angielski: The modern programs of the monitoring of alimentary tract's diseases in outpatient's practice of a general practitioner.
Autorzy: Chernobrovijj V. M., Melashhenko S. G., Palihjj Ih. G., Pihvtorak N. A., Zajika S. V., Skripka A. A., Fedzhaga Ih. V., Kavka S. O., Kosenko S. V., Fostakovs'kijj D. S., Chernova Ih. V., Morozova O. Ih., Burka N. V., Kolihsnik S. P.
Źródło: New Med. 2002: 5 (1) s.16-20, bibliogr. 9 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The article is devoted to the experience of treatment widespread alimentary tract's diseases in outpatient's practice by using methods of control gastrointestinal disorders: acid-related (hyperacidity, hypoacidity, gastroesofageal and duodenogastral reflux) and nonacid-related (contamination of microogranisms, vertebrogenic).

21/2523

Tytuł oryginału: Rol' sihmejjnoji medicini v optimihzacihij roboti po profihlakticih gostrikh porushen' mozkovogo krovoobihgu.
Tytuł angielski: The role of family medicine in optimization of prevention of acute cerebral circulatory disturbans.
Autorzy: Pulik O. R., Chopejj I. V., Pulik R. O.
Źródło: New Med. 2002: 5 (1) s.21-23, bibliogr. 9 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: In the article the various models of optimization of prevention of acute cerebral circulatory disturbances are presented. The significant role of the "patient's diary" has been shown.

22/2523

Tytuł oryginału: Ocihnka antigihpertenzivnoji dihji betaksolola (lokrenu) v gostrikh farmakodinamihchnikh testakh u khvorikh na arterihal'nu gihpertenzihju, shho perenesli ihnfarkt mihokarda (za danimi dobovogo monihtoruvannja arterihal'nogo tisku).
Tytuł angielski: The studing of arterial blood pressure monitoring results under the influence of lokren revealed its significant antihypertensive efficacy both after the first 20 mg dose and after 2-weeks course of treatment.
Autorzy: Vakaljuk I. P., Stasjuk L. B., Zvonar P. P., Lihbrik O. M.
Źródło: New Med. 2002: 5 (1) s.24-27, il., bibliogr. 8 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: The estimation of antihypertensive efficacy of lokren during the pharmonodynamic tests in patients with arterial hypertension, after acute myocardial infraction (by data of arterial blood pressure monitoring). While treating patients with 20 mg of Lokren the decreasing of systolic and diastolic arterial blood pressure variability and heart rate was observed.

23/2523

Tytuł oryginału: Analihz vrodzhenikh vad rozvitku plodu v oblasnomu centrih Zakarpattja ta jikh znachennja v prakticih sihmejjnogo lihkarja.
Tytuł angielski: Some data of ultrasonic investigation of pregnant women and analysis of natal defects of fetus during the time period of 1993-1999 in the town of uzhgorod.
Autorzy: Chopejj O. Ih., Slivka N. V., Chopejj K. Ih.
Źródło: New Med. 2002: 5 (1) s.28-31, tab., bibliogr. 5 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

Streszczenie angielskie: We studied the data of prenatal ultrasound diagnostics of abnormalities in fetus. Coincidence of prenatal and postnatal diagnoses was registered in 92 p.c. Structure and index of rate of abnormalities in fetus were compared with the data of monitoring in newborns. The differences have been analyzed.

24/2523

Tytuł oryginału: Optimihzacihja navchal'nogo procesu lihkarihv zagal'noji praktiki na ciklih "kardihologihja" shljakhom vikoristannja suchasnikh ihnformacihjjnikh tekhnologihjj.
Autorzy: Glushko L. V., Fedorov S. V., Makovec'ka T. Ih., Ostrovs'kijj M. M., Berezovs'ka L. A.
Źródło: New Med. 2002: 5 (1) s.32-33, bibliogr. 3 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The demands made to quality of training physicians to be are defined in the programs and directives of the Ministry of Public Health and Education of Ukraine. One of the factors directed to profound medical subjects studying is subsequent optimizing the training process, namely raising use of information technologies in teaching. This article gives facts and figures concerning forming postgraduate medical training of general practicioners in cardiology cycle using the modern information technologies.

25/2523

Tytuł oryginału: Rezultati vivchennja pokaznikihv pH slizovoji obolonki tovstoji kishki ta vikoristannja v klihnihchnihjj prakticih jikh zmihni pihd vplivom lihkuvannja.
Tytuł angielski: PH-metry in the patients having irritable bowel syndrome and variable pH after treatment.
Autorzy: Rusin Ih. S., Ihl'ko A. V., Ploskihna V. Ju., Kanchihjj V. M., Rihshko V. V.
Źródło: New Med. 2002: 5 (1) s.34-35, tab., bibliogr. 4 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The methods and the results of transcolonoscopic pH-metria of the different parts of bowels, changes of this indices attached to diseases of this part of the gastrointestinal tract under influence of different complexes of treatment are being discussed in this publication.

26/2523

Tytuł oryginału: Docihl'nihst' vikoristannja al'ternativnikh metodihv lihkuvannja khvorikh kardihologihchnogo profihlju v prakticih sihmejjnogo lihkarja.
Tytuł angielski: The expediency of alternative treatment methods used in a family doctor's practice.
Autorzy: Shkoda-Ul'janova N. V., Dolgosh M. Ju., Panas S. V.
Źródło: New Med. 2002: 5 (1) s.36-39, il., tab., bibliogr. 9 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Taking into consideration a significant number of the adverse effects, which appear after the treatment of the cardio - vascular diseases, namely: 1) tolerance; 2) cancellation syndrome; 3) paradoxical effect; it is suggested, as an alternative, to use angio - injeel, which could be applied both independently, as a monotherapy, and with other traditional remedies. The dosage, in this case, is decreased, contributing to the less number of cases of the aversive effects.

27/2523

Tytuł oryginału: Vivchennja ta mozhlivostih lihkuvannja gelihkobakterihozy v rodinakh khvorikh z urazhennjam gastrodyodenal'noji zoni.
Tytuł angielski: Study and opportunity of helicobacteriosis treatment in families of the patients with gastroduodenal disorders.
Autorzy: Kolesnik P. O., Chopejj Ih. V., Rubcova Je. Ih., Dolgosh M. Ju., Drozd S. Ih., Knishev V. Ih., Kharitonova Z. B., Kuz'mik V. M.
Źródło: New Med. 2002: 5 (1) s.40-44, bibliogr. 12 poz., sum.
Sygnatura GBL: 313,682

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The main theme of the article contains analysis of intrafamiliar clustering and transmission which is implicated as a major route for acquisition of Helicobacter pylori infection. The occurrence of digestive disorders in household members of the patients with chronic gastritis and duodenal ulcer disease families was observed by clinical and instrumental investigation. It proves the high risk of digestive disorders, associated with Helicobacter pylori, in the household members of the index patients. The results of the analysis prove the necessity of all-family eradication treatment in order to avoid reinfection, intrafamiliar clustering and transmission of Helicobacter pylori infection. It can also reduce the risk of digestive disorders, associated with Helicobacter pylori, in the household members of the index patients. The household members and the index patients treatment by the schedule list (which included omeprasole, augmentine, cyprofloxacine during 7 days) caused high eradication effect observed by invasive tests and was mostly alwaysaccompanied by changes in Ig G titres.

28/2523

2002
Tytuł polski: Probabilistic causal models in medicine: application to diagnosis of liver disorders : praca doktorska
Autorzy: Oniśko Agnieszka, Bobrowski Leon (promot.).; [Akademia Medyczna w Białymstoku]
Źródło: 2002, [1], X, 140 k. : il., tab., bibliogr. 158 poz., sum., maszyn.
Sygnatura GBL: 45/23902

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

29/2523

Tytuł oryginału: Medical humanities in Central and Eastern Europe : the example of Poland
Autorzy: Szawarski Zbigniew
Źródło: W: Integration of medical humanities into the education of health care professionals - Vilnius, 2002 s.17-23 - Włączenie humanistyki medycznej do kształcenia personelu służby zdrowia Wilno 28-29.06. 2002 - Oddzielna numeracja stron tekstu litewskiego w odwrotnym kierunkuTytuł odwróconej odkładki także w jęz. litewskim
Sygnatura GBL: 555,550

Typ dokumentu:

30/2523

Tytuł oryginału: Assessment of long-term effects of exposure to toluene based on the analysis of selected behavioral responses with particular reference to the ability to trigger behavioral hypersensitivity in rats.
Autorzy: Wiaderna Dorota, Tomas Tadeusz
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.239-245, il., bibliogr. 20 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Toluene is a major component of numerous commercial organic solvent formulations. It is often listed among the chemicals capable of producing the organic solvent syndrome and a neurobehavioral hypersensitivity condition. The hypersensitivity condition (continued long-term intensification of some behavioral reactions in response to pharmacological or environmental stressor) is usually associated with the increased tonus of the functional dopaminergic system. The aim of our current research was to determine whether, under conditions of inhalation exposure, toluene can produce long-term behavioral changes or modify the intensity of the behavioral response to apomorphine, a dopaminergic receptor agonist. In our experiment, male rats were exposed to 25, 100 and 250 ppm toluene for 4 weeks (5 days/week, 6h/day). The following behaviors were tested: finding water in a radial maze; open field motor activity; acquiring the conditional response of passive avoidance; sensitivity to a thermal pain stimulus (hot plate test) and changes in this sensitivity caused by stress; and acquiring the conditional response of two-directional active avoidance. The behavioral response to apomorphine, i.e. the increased spontaneous locomotor activity, was assessed on day 10 after the termination of the exposure in the rotary drum test. In the behavioral experiment, significant differences between groups were recorded only for hot plate test; in the 100 and 250 ppm rats, electric-shock-related anxiety response was stronger than in the control group. In the experiment using pharmacological provocation...

31/2523

Tytuł oryginału: Effects of occupational exposure to a mixture of solvents on the inner ear: a field study.
Autorzy: Sułkowski Wiesław J., Kowalska Sylwia, Matyja Wojciech, Guzek Wojciech, Wesołowski Wiktor, Szymczak Wiesław, Kostrzewski Przemysław
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.247-256, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Some clinical and laboratory studies that industrial solvents such as toluene, styrene, xylene, trichloroethylene and carbon disulfide or their mixtures may affect the inner ear, although the mechanism of this process is still not well understood. The aim of this investigation was to assess the incidence of hearing and vestibular disorders (using modern audiological and vestibular tests) in 61 workers exposed to a mixture solvents at the production of paints and varnishes; the control group included 40 age-matched non-exposed subjects. Environmental and biological monitoring revealed that the most significant exposure can be attributed to the following mixture constituents: ethylbenzene, xylene and trimethylbenzene isomers such as pseudocumene, mesitylene and hemimellitene. Electronystagmographic examinations showed the symptoms of vestibular dysfunction, as well as the decreased duration, amplitude and slow phase angular velocity of induced nystagmus in 47.5 p.c. of the subjects exposed versus 5 p.c. of controls. This was accompanied by sensorineural high frequency hearing loss, identified by means of pure tone audiometry in 42 p.c. of those exposed versus 5 p.v. controls, and reduced amplitudes of transiently evoked and distoration-product otoacoustic emissions. The findings closely correspond with the rate total exposure to the solvent mixture. A possible mechanism responsible for ototoxicity of solvents is discussed.

32/2523

Tytuł oryginału: Hospitalizations among teachers in Denmark, 1981-1997.
Autorzy: Hannerz Harald, Albertsen Karen, Tchsen Finn
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.257-266, il., tab., bibliogr. 61 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

medycyna pracy

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: ll systems and organs of the body were statistically significant lower among teachers compared with other economically active people of Denmark.

Streszczenie angielskie: It is a common notion that teachers constitute a group at high risk of occupational stress, but the potential health consequences of the assumed high stress levels are still poorly illuminated. The aim of the present study was to investigate morbidity among Danish teachers. Cohorts of Danish teachers of primary, secondary and vocational schools, aged 20-59 years in 1981, 1986, 1991 and 1994, were formed to calculate age standardized hospitalization ratios (SHRs) and time trends (1981-1997) for a large variety of diagnostic aggregations. Statistically significantly low SHRs for the major disease categories: neoplasms, diseases of the nervous, circulatory, respiratory, digestive and musculoskeletal systems were found both for man and women. We also found significantly low SHRs for the aggregated diagnostic group "injury and poisoning" in men and for diseases of the nervous and sense organs in women. Age standardized hospitalization rates with respect to diseases observed in practically all systems and organs of the body were statistically significantly lower among teachers compared with other economically active people of Denmark.

33/2523

Tytuł oryginału: Mortality study of workers compensated for asbestosis in Poland, 1970-1997.
Autorzy: Szeszenia-Dąbrowska Neonila, Wilczyńska Urszula, Szymczak Wiesław, Strzelecka Alicja
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.267-278, tab., bibliogr. 21 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was to assess the risk of asbestos-related malignancies among persons with diagnosed asbestosis. The study covered a cohort composed of 907 men and 490 women afficted by asbestosis, diagnosed in 1970-1997. The follow-up of the cohort continued until 31 December 1999. In all, 421 deaths were registered and causes of death were retrieved for 93.3 p.c. of the deceased. A significantly increased mortality was observed both in the male (300 deaths; SMR = 127; 95 p.c. CI: 113-142) and female (121 deaths, SMR = 150; 95 p.c. CI: 124-179) cohorts. The elevated number of deaths in the male and female cohorts were noted mainly due to respiratory diseases (men: 42 deaths; SMR = 344; 95 p.c. CI: 248-465 women: 20 deaths, SMR = 789; 95 p.c. CI: 482-1219) malignant neoplasms (men: 91 deaths, SMR = 146; 95 p.c. CI: 118-179; women: 34 deaths, SMR = 159; 95 p.c. CI: 110-222), including lung cancer (men: 39 deaths, SMR = 168; 95 p.c. CI: 119-230; women: 13 deaths, SMR = 621; 95 p.c. CI: 331-1062) and pleural mesothelioma (men: 3 deaths, SMR = 2680; 95 p.c. CI: 553-7832; women: 3 deaths, SMR - 7207; 95 p.c. CI: 1031-14612). Taking into account a cumulative dose of fibres, it was found that a significantly increased mortality from lung cancer and pleural mesothelioma applied to persons exposed to a dose above 25 f-y/ml. The results indicate that persons with asbestosis are at higher risk of developing malignant neoplasms, especially lung cancer and mesothelioma.

34/2523

Tytuł oryginału: Lung function and surface electromyography of intercostal muscles in cement mill workers.
Autorzy: Meo Sultan A., Azeem Muhammad A., Ghori Moinuddin G., Subhan Mirza M. F.
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.279-287, tab., bibliogr. 48 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Impairment of pulmonary function in cement mill workers has been previously reported without considering a variety of parameters that can help evaluate more thoroughly the effect of cement dust on the respiratory system. In addition, an integrated approach has not been considered to assert the involvement of respiratory muscles. Therefore, in the present study spirometry and surface electromyography (SEMG) of intercostal muscles were used for indicating pulmonary impairment. In this study, a group of 50, apparently healthy volunteers, male cement mill workers aged 20-60 years with exposure of 13 years on average, were randomly selected. They were matched with another group of 50 control healthy male subjects in terms of age, height, weight and socioeconomic status. Both groups met the standard exclusion criteria. Spirometry was performed on an electronic spirometer, while SEMG of intercostal muscles was performed by using a chart recorder. The results demonstrated statistically significant reduction in lung function parameters i.e., force vital capacity (FVC) (p 0.0005); force expiratory volume in first second (FEV1) (p 0.0005); peak expiratory flow (PEF) (p 0.005); and maximum voluntary ventilation (MVV) (p 0.0005) in cement mill workers, when compared with controls. However, the FEV1/FVC ratio was significantly higher (p 0.025) in cement mill workers. Similarly, the parameters obtained from SEMG of intercostal muscles, i.e. number of peaks (NPO) (p 0.0005); maximum peak amplitude (MPA) (p 0.0005); peak to peak amplitude (PPA) (p 0.0005)...

35/2523

Tytuł oryginału: Occupational exposure to organic dust associated with municipal waste collection and management.
Autorzy: Krajewski Jan A., Tarkowski Stanisław, Cyprowski Marcin, Szarapińska-Kwaszewska Jadwiga, Dudkiewicz Bożena
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (3) s.289-301, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was to assess the occupational exposure of workers employed in the municipal waste collection and management industry. Air samples were collected in the workers' breathing zone; two samples were collected parallely. The assessment concerned exposure to organic dust, endotoxins, bacteria and fungi. The questionnaire data showed that workers found themselves in good or very good health. They also regarded noise, dustiness, odor, physical effort and changeable atmospheric conditions as the most strenuous factors. The highest dust concentrations were observed on the sites of waste collection (mean, 7.7 mg/m3) and composting (mean, 4.6 mg/m3). Samples collected on the composting site contained the largest amount of endotoxins (mean concentration, 76 ng/m3). This was followed by sorting facility (61 ng/m3) and waste collection area (36 ng/m3). Gram-negative rods (primarily intestinal) were found in all samples collected at workposts, which justified the adoption of the air concentrations of endotoxins as a criterion for assessing their hygienic conditions. Taking MAC for total suspended dust (4.0 mg/m3) and the concentration of 10 ng/m3 for endotoxin as the criteria for the exposure evaluation, it must be considered that waste collectors and composting site workers are working in poor hygienic conditions. Workers employed at the most hazardous workposts did not use personal protective equipment (glasses, antirespirators), thus infringing a fundamental condition for limiting health hazards (Directive 2000/54/EC).

36/2523

Tytuł oryginału: Remarks on safety assessment, quality assurance, and diagnostic reference levels in medical X-ray imaging.
Autorzy: Golnik Natalia, Ratajczyk-Dziewięcka Małgorzata, Maciejewski Krzysztof
Źródło: Pol. J. Med. Phys. Engin. 2002: 8 (1) s.15-22, bibliogr. 10 poz.
Sygnatura GBL: 306,499

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The new Polish legislation (Atomic Law) and recent recommendations of international organizations require to pay more attention to control the risk associated with the operation of medical radiation sources. A comprehensive regulatory framework is necessary to ensure that a consistent approach is applied to all elements of the safety system. The paper shortly describes how safety assessment fits into the regulatory process and provides some information on the elements of a safety assessment. Complementary to the safety assessment, a program of quality assurance, QA, should be implemented to periodically check all aparameters which influence the patient dose and the image quality. The last part of the paper concern the implementation of diagnostic reference levels, recommended as a practical tool to mange radiation dose to patients in diagnostic radiology and nuclear medicine.

37/2523

Tytuł oryginału: On the acoustic phenomena produced by turbulence in the flowing blood.
Autorzy: Góral-Wójcicka A., Borgieł W., Małota Z., Nawrat Z.
Źródło: Pol. J. Med. Phys. Engin. 2002: 8 (1) s.29-35, il., bibliogr. 12 poz.
Sygnatura GBL: 306,499

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: A turbulent flow can be a source of sounds called "murmur" or "souffle", and can be a result of pathology in the blood vascular system. A model of acoustical effects, related to stenosis of the aorta, was created. Numerical calculations of pressure fluctuations in the environment of a simulated turbulent region were performed.

38/2523

Tytuł oryginału: Dosimetric verification of dose distributions calculated for Co-60 beams with individual compensation filters.
Autorzy: Perczak Aneta, Kukołowicz Paweł, Bulski Wojciech
Źródło: Pol. J. Med. Phys. Engin. 2002: 8 (1) s.49-61, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 306,499

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The compensating filters used in radiotherapy modify dose distribution in the entire field of the radiation beam, no matter what is the shape of the surface of the body. The determination of the shape of the compensator in a given clinical siluation calls for the use of advanced software tools. Modern treatment planning systems provide necessary modules needed to calculate the shapes of the compensation filters. Reliable use of these systems requires verification of such calculations. In this study, in order to evaluate the accuracy of the calculations, the dose distributions for Co-60 beams modified by the compensators, calculated by the computer system TMS-Helax were verified by comparing them with the measured dose distributions.

39/2523

Tytuł oryginału: The immunophenotypic and immunogenotypic B-cell differentiation arrest in bone marrow of RAG-deficient SCID patients corresponds to residual recombination activities of mutated RAG proteins.
Autorzy: Noordzij Jeroen G., Bruin-Versteeg Sandra de, Verkaik Nicole S., Vossen Jaak M. J. J., Groot Ronald de, Bernatowska Ewa, Langerak Anton W., Gent Dik C. van, Dongen Jacques J. M. van
Źródło: Blood 2002: 100 (6) s.2145-2152, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The protein products of the recombination activating genes (RAG1 and RAG2) initiate the formation of immunoglobulin (lg) and T-cell receptors, whichare essential for B- and t-cell development, respectively. Mutations in the RAG genes result in severe combined immunodeficiency disease (SCID), generally characterized by the absence of mature B and T lymphocytes, but presence of natural killer (NK) cells. Biochemically, mutations in the RAG genes result either in nonfunctional proteins or in proteins with partial recombination activity. The mutated RAG genes of 9 patients from 7 families were analyzed for their recombination activity using extrachromosomal recombination substrates, rearrangment of endogenous Ig loci in RAG gene-transfected nonlymphoid cells, or the presence of Ig gene rearrangements in bone marrow (BM). Recombination activity was virtually absent in all 6 patients with mutations in the RAG core domains, but partial activity was present in the other 3 RAG-deficient patients, 2 of them having Omenn syndrome with oligocional T lymphocytes. Using 4-color flow cytometry, we could define the exact stage at which B-cell differentiation was arrested in the BM of 5 RAG-deficient SCID patients. In 4 of 5 patients, the absence of recombination activity was associated with a complete B-cell differentiation arrest at the transition from cytoplasmic (Cy) Igć- pre -B-I cells to Cylgć+ pre -B-II cells. However, the fifth patient showed low frequencies of precursor B cells with Cylgć and surface membrane IgM...

40/2523

Tytuł oryginału: Comparative analysis of Ig and TCR gene rearrangements at diagnosis and at relapse of childhood precursor-B-ALL provides improved strategies for selection of stable PCR targets for monitoring of minimal residual disease.
Autorzy: Szczepański Tomasz, Willemse Marja J., Brinkhof Bas, Wering Elisabeth R. van, Burg Mirjam van der, Dongen Jacques J. M. van
Źródło: Blood 2002: 99 (7) s.2315-2323, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements are excellent patient-specific polymerase chain reaction (PCR) targets for detection of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL), but they might be unstable during the disease course. Therefore, we performed detailed molecular studies in 96 childhood precusor-B-ALL at diagnosis and at relapse (n = 91) or at presumably secondary acute myeloid leukemia (n = 5). Clonal Ig and TCR tatgets for MRD detection were identified in 94 patients, with 71 p.c. of these targets being preserved at relapse. The best stability was found for IGK-Kde rearrangements (90 p.c.), followed by TCRG (75 p.c.), IGH (64 p.c.), and incomplete TCRD rearrangements (63 p.c.). Combined Southern blot and PCR data for IGH, IGK-Kde, and TCRD genes showed significant differences in stability at relapse between monoclonal and oligoclonal rearrangements: 89 p.c. versus 40 p.c., respectively. In 38 p.c. of patients all MRD-PCR targets were preserved at relapse, and in 40 p.c. most of the targets (ň 50 p.c.) were preserved. In 22 p.c. of patients most targets (10 cases) or all targets (10 cases) were lost at relapse. The latter 10 cases included 4 patients with secondary acute myeloid leukemia with germiline Ig/TCR genes. In 5 other patients additional analyses proved the clonal relationship between both disease stages. Finally, in 1 patient all Ig/TCR gene rearrangements were completely different between diagnosis and relapse, which is suggestive of secondary ALL...

41/2523

Tytuł oryginału: Human leukocyte antigens class II and tumor necrosis factor genetic polymorphisms are independent predictors of non-Hodgkin lymphoma outcome.
Autorzy: Juszyczyński Przemysław, Kalinka Ewa, Bienvenu Jacques, Woszczek Grzegorz, Borowiec Maciej, Robak Tadeusz, Kowalski Marek, Lech-Maranda Ewa, Baseggio Lucile, Coiffier Bertrand, Salles Gilles, Warzocha Krzysztof
Źródło: Blood 2002: 100 (8) s.3037-3040, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: at least 2 inherited factors involved in NHL outcome.

Streszczenie angielskie: Tumor necrosis factor (TNF) production and non-Hodgkin lymphoma (NHL) outcome was found to be related to the TNF-308 polymorphism. To explore whether this could be linked to neighboring polymorphisms, we genotyped the TNF-376,-308,-238,-163, lymphotoxin alpha (LTŕ)+252, and HLA DRB1 alleles in 204 patients with NHL and 120 controls. TNF-308A was the only allele associated with higher TNF and its p55 and p75 receptors' levels (P=.009, P=.03, and P=.007) and lower complete remission rates (P=.006). Freedom from progession (FFP) and overall survival (OS) were shorter in patients with TNF-308A (P=.009 and P=.02), null HLA DRB1*02 allele (P=.007 and P.14) or both genetic markers (P=.004 and P.005). Multivariate analysis incorporating international. Prognostic index (IPI) identified TNF-308A (P .0001, relative risk (RR) = 1.63; P .0001, RR=1.51) and null HLA DRB1*02 alleles (P=.015, RR - 1.18; P .0001, RR = 1.25) as independent factors for FFP and OS. These results indicate the existence of least 2 inherited factors involved in NHL outcome. (Blood. 2002; 100 : 3037-3040).

42/2523

Tytuł oryginału: Modulation of the activity of calcium-activated neutral proteases (calpains) in chronic lymphocytic leukemia (B-CLL) cells.
Autorzy: Witkowski Jacek M., Żmuda-Trzebiatowska Emilia, Świercz Jakub M., Cichorek Mirosława, Ciepluch Hanna, Lewandowski Krzysztof, Bryl Ewa, Hellmann Andrzej
Źródło: Blood 2002: 100 (5) s.1802-1809, il., bibliogr. 57 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Decreased susceptibility to apoptosis and impaired proliferative control are throught to be responsible for prolonged life spain and accumulation of chronic lymphocytic leukaemia (B-CLL) cells. The acitivity of calpains (calcium-dependent, neutral proteases, active in the cells responding to signals inducing a rise of cytoplasmic (Ca++) is involed in the regulation of apoptosis of some cell types by interaction with caspase-3. This work verifies the hypothesis of the abnormal activity of calpains and its role in reduced apoptosis of the BCL cells. Casein zymography, reverse transcriptase-polymerase chain reaction, and Western blotting were used for identification and quantification of the activity and expression of calpains (calcium-dependent, neutral proteases, active in the cells responding to signals inducing a rise of cytoplamic (Ca++) is involved in the regulation of apoptosis of some cell types by interaction with caspase-3. This work verifies the hypothesis of the abnormal activity of calpains and its role in reduced apoptosis of the B-CLL cells. Casein zymography, reverse transcriptase-polymerase chain reaction, and Western blotting were used for identification and quantification of the activity and expression of calpains in B-CLL cells and purified B lymphocytes. The activity and expression of ć-calpain (requiring micromolar Ca++ for activation) are significantly higher in the leukemic than in nonmalignant cells. Conctrarily, the activity and expression of m-calpain (requiring milimolar Ca++) as well as the expression of calpastatin (an endogenous inhibitor of...

43/2523

Tytuł oryginału: Outcome of adult patients with T-lymphoblastic lymphoma treated according to protocols for acute lymphoblastic leukemia.
Autorzy: Hoelzer Dieter, Gkbuget Nicola, Digel Werner, Faak Thomas, Kneba Michael, Reutzel Regina, Romejko-Jarosińska Joanna, Zwoliński Jacek, Walewski Jan
Źródło: Blood 2002: 99 (12) s.4379-4395, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We treated 45 adult patients with T-lymphoblastic lymphoma (T-LBL) (age range 15-61 years) with 2 protocols designed for adult acute lymphoblastic leukemia (ALL). An encouraging cure rate of 90 p.c. was recently reported for T-LBL in children treated with a similar approach. In our study an 8-drug standard induction was administered over 8 weeks including prophylactic cranial (24 Gy) and mediastinal irradiation(24 Gy) followed by consolidation and reinduction therapy. AT diagnosis, 91 p.c. of the 45 patients showed a mediastinal tumor and 40 p.c. had pleural/pericardial effusions: 73 p.c. of the patients had stage III/IV disease. Ovarall 42 patients (93 p.c.) achieved a complete remission (CR), 2 patients (4 p.c.) achieved a partial remission, and 1 patient (2 p.c.) died during induction. In patients with stage I-III disease (n=18) the CR rate was 100 p.c. compared with 89 p.c. in stage IV (n=27). There were 15 patients who relapsed (36 p.c.) within 12-months. The majority of relapses (47 p.c.) occurred in the mediastinum (n=7) despite mediastinal irradiation with 24 Gy in 6 out of 7 patients. The estimates for overall survival, continuous CR, and disease-free survival at 7 years are 51 p.c., 65 p.c., and 62 p.c., respectively. Stage, age, lactate dehydrogenase and all other parameters had no influence on achievement of CR or outcome. This study demonstrates in a large cohort of patients with adult T-LBL that a high CR rate and a favourable outcome can be achieved with an ALL-type regimen. Mediastinal recurrence was the major obstacle and further improvement...

44/2523

Tytuł oryginału: Complementary functions of the antiapoptotic protein A1 and serine/threonine kinase pim-1 in the BCR/ABL-mediated leukemogenesis.
Autorzy: Nieborowska-Skorska Małgorzata, Hoser Grażyna, Kossev Plamen, Wasik Mariusz A., Skorski Tomasz
Źródło: Blood 2002: 99 (12) s.4531-4539, il., bibliogr. 68 poz.
Sygnatura GBL: 301,770

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: BCR/ABL oncogenic tyrosine kinase activates STAT5, which plays an important role in leukemogenesis. The downstream effectors of the BCR/ABL- STAT5 pathway remain poorly defined. We show here that expression of the antiapoptic protein A1, a member of the Bcl-2 family, and the serine/threonine kinase pim-1 are enhanced by CRB/ABL. This up-regulation requires activation of STAT5 by the signaling from SH3 + SH2 domains of the BCR/ABL. Enhanced expression of A1 and pim-1 played a key role in the BCR/ABL-mediated cell protection from apoptosis. In addition, pim-1 promoted proliferation of the BCR/ABL-transformed cells. Both A1 and pim-1 were required to induce interleukin 3-independent cell growth, inhibit activation of caspase 3, and stimulate cell cycle progression. Moreover, simultaneous up-regulation of both A1 and pim-1 was essential for in vitro transformation and in vivo leukemogenesis mediated by BCR/ABL. These data indicate that induction of A1 and pim-1 expression may play a critical role in the BCR/ABL - depenendent transformation.

45/2523

Tytuł oryginału: Early detection of COPD by high-risk population spirometric screening.
Autorzy: Zieliński J.
Źródło: Eur. Respir. Rev. 2002: 12 (86/87) s.287-288
Sygnatura GBL: 313,671

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

46/2523

Tytuł oryginału: Subtle acquired renal injury as a mechanism of salt-sensitive hypertension
Autorzy: Johnson Richard J., Herrera-Acosta Jaime, Schreiner George F., Rodriguez-Iturbe Bernardo
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[23-33], il., bibliogr. 94 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

47/2523

Tytuł oryginału: The vascular endothelin system is not overactive in normotensive hemodialysis patients
Autorzy: Bssemaker Eckhart, Passauer Jens, Reimann Doreen, Schulze Beate, Reichel Wolfgang, Gross Peter
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[59-67], il., tab., bibliogr. 30 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

48/2523

Tytuł oryginału: Ageing and heart failure : the place of ACE inhibitors in heart failure with preserved systolic function
Autorzy: Tendera M[ichał]
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[69-75], il., tab., bibliogr. 54 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

49/2523

Tytuł oryginału: Chronic hypokalaemia of adults : Gitelman's syndrome is frequent but classical Bartter's syndrome is rare
Autorzy: Gladziwa U., Schwarz R., Gitter A. H., Bijman J., Seyberth H., Beck F., Ritz E, Gross P[eter]
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[81-87], il., tab., bibliogr. 22 poz., sum. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

50/2523

Tytuł oryginału: Chronic, diagnosis-resistant hypokalaemia
Autorzy: Reimann Doreen, Gross Peter
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[89-93], il., tab., bibliogr. 30 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

51/2523

Tytuł oryginału: Oral antihyperglycemic therapy for type 2 diabetes
Autorzy: Inzucchi Silvio E.
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[127-139], il., tab., bibliogr. 154 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

52/2523

Tytuł oryginału: Insulin therapy in type 2 diabetes
Autorzy: Hollander Priscilla
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[141-146], il., tab., bibliogr. 36 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

53/2523

Tytuł oryginału: Advances in the treatment of renal anaemia
Autorzy: Locatelli Francesco
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[147-160], il. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

54/2523

Tytuł oryginału: HMG-CoA reductase inhibitors are associated with reduced mortality in ESRD patients
Autorzy: Seliger Stephen L., Weiss Noel S., Gillen Daniel L., Kestenbaum Bryan, Ball Adrianne, Sherrard Donald J., Stehman-Breen Catherine O.
Źródło: W: 2 Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[171-178], il., tab., bibliogr. 31 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

55/2523

Tytuł oryginału: Atherogenic lipoproteins in end-stage renal disease
Autorzy: Shoji Tetsuo, Ishimura Eiji, Inaba Masaaki, Tabata Tsutomu, Nishizawa Yoshiki
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[179-182], il., bibliogr. 23 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

56/2523

Tytuł oryginału: Tailoring the initial vascular access for dialysis patients
Autorzy: Konner Klaus, Hulbert-Shearon Tempie E., Roys Erik C., Port Friedrich K.
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[235-244], il., tab., bibliogr. 28 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

57/2523

Tytuł oryginału: Peritonitis in peritoneal dialysis
Autorzy: Voinescu C. G., Khanna R.
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[245-260], tab., bibliogr. 94 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

58/2523

Tytuł oryginału: The malnutrition, inflammation, and atherosclerosis (MIA) syndrome : the heart of the matter
Autorzy: Pecoits-Filho Roberto, Lindholm Bengt, Stenvinkel Peter
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[283-286], il., bibliogr. 34 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

59/2523

Tytuł oryginału: Principles and practice of renal transplantation
Autorzy: Kahan Barry D., Ponticelli Claudio
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[303-321], il., tab. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

60/2523

Tytuł oryginału: Treatment of secondary hyperparathyroidism with vitamin D derivatives and calcimimetics before and after start of dialysis
Autorzy: Dreke Tilman B.
Źródło: W: 2. Katowickie Seminarium "Postępy w nefrologii i nadciśnieniu tętniczym" : [materiały zjazdowe] - Katowice, [2002] s.[337-339], bibliogr. 23 poz. - 2 Katowickie Seminarium pt. Postępy w nefrologii i nadciśnieniu tętniczym Katowice 14-16.11. 2002
Sygnatura GBL: 802,649

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

61/2523

Tytuł oryginału: Influence of intensive movement rehabilitation in scout camps on ventilatory parameters of the respiratory tract in children with mild idiopathic scoliosis from highly polluted areas.
Tytuł polski: Wpływ intensywnego usprawniania ruchowego podczas obozu harcerskiego na czynność układu oddechowego u dzieci ze skoliozą idiopatyczną, pochodzących ze środowiska skażonego.
Autorzy: Żaba Ryszard
Źródło: Wiad. Lek. 2002: 55 supl. 1 cz. 2: Konferencja naukowa "Śląskie dni medycyny społecznej i zdrowia publicznego" s.1003-1008, tab., bibliogr. 19 poz., streszcz. - Konferencja naukowa pt. Śląskie dni medycyny społecznej i zdrowia publicznego Zabrze - Szczyrk 2002
Sygnatura GBL: 301,774

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Przebadano grupę 42 dzieci ze skoliozą idiopatyczną I stopnia wg Cobba (skrzywienie kręgosłupa poniżej 30 stopni) oraz grupę kontrolną 14 zdrowych dzieci biorących uddział w harcerskim obozie zdrowotnym (Obóz I), jak również przebadano grupę 19 dzieci ze skoliozą I stopnia, która po raz drugi uczestniczyła w harcerskim obozie rehabilitacyjnym (Obóz II). U wszystkich badanych dzieci, zarówno przed jak i po pobycie na 4 tygodniowym obozie rehabilitacyjnym oznaczono aparatem Vitalograph następujące wskaźniki wentylacyjne płuc: pojemność życiową płuc - FVC, maksymalną objętość wydechową jednosekundową - FEV1 oraz określono FEV1 proc. FVC. Porównując średnie wartości mierzonych wskaźników spirometrycznych płuc przed i po pobycie na pierwszym Obozie Rehabilitacyjnym, nie wykazano statystycznie znamiennych przyrostów. Równocześnie porównując mierzone wskaźniki wentylacyjne FVC i FEV1 po pobycie na drugim Obozie Rehabilitacyjnym, wykazano statystycznie znamienne ich przyrosty.

Streszczenie angielskie: In 42 children with mild idiopathic scoliosis Iř (spinal curvature less than 30ř) and in the control - 14 healthy children (camp I) as well as in 19 children with idiopathic scoliosis spending time in a camp for the second time (camp II) ventilatory lung parameters: vital capacity - VC, maximal forced expiratory volume one sec. - FEV1 and FEV1 p.c. FVC were determined. Tests were carried out with use of Vitalograph before and after four-week rehabilitation camp. When the mean values of spirometric parameters were compared between the two groups of children (camp I) before and after the camp, statistically significant values were not observed. Simultaneously, in children staying in the camp second time around (camp II), when the mean values of spirometric parameters were correlated between the two camps as well as before and after the second camp, statistically significant increased values of parameters were observed. Leaving Silesia, an area with high air pollution is one of the major tasks of Scout Health Camps for children with idiopathic scoliosis.

62/2523

Tytuł oryginału: Inhibition of alkaline phosphatase activity of boar semen by pentoxifylline, caffeine, and theophylline.
Autorzy: Głogowski Jan, Danforth Douglas R., Ciereszko Andrzej
Źródło: J. Androl. 2002: 23 (6) s.783-792, il., bibliogr. s. 790-792
Sygnatura GBL: 313,131

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Methyl xanthines have been used frequently as additives to sperm suspensions in order to improve sper characteristics. The mechanism of action on spermatozoa is generally assumed to be inhibition of sperm phospodiesterase activity, resulting in elevation of complementary adenosine monophosphate levels in spermatozoa. The present study was designed to examine the effect of methyl xanthines (pentoxifylline, caffeine, and theophylline) on another important enzyme system, alkaline phosphatase, in boar seminal plasma and spermatozoa. Inhibition of sperm alkaline phosphatase could be distinguished from that of seminal plasma by a paradoxical stimulation by pentoxifylline at lower pH values in spermatozoa. Among the three methyl xanthines, theophylline exhibited the most dramatic inhibition of alkaline phosphatase activity and substrate inhibition was observed with increasing concentrations. Each methyl xanthine had a different action on alkaline phosphatase activity at lower pH; theophylline showed the highest inhibition, caffeine inhibition was not related to pH, and pentoxifylline did not inhibit alkaline phosphatase of seminal plasma and, in fact, it stimulated its activity (or that of a phosphatase with lower pH optimum) in spermatozoa. These results indicate another possible mechanism of action of methyl xanthines on sperm and are in agreement with data indicating that methyl xanthines are not specific inhibitors of sperm phosphodiesterase, because clearly, they inhibit alkaline phosphatase activity as well.

63/2523

Tytuł oryginału: Bacterial infections - an early detection and cellular defense.
Tytuł polski: Zakażenia bakteryjne - wczesna diagnostyka i odporność komórkowa.
Autorzy: Maliński Tadeusz
Źródło: Nowiny Lek. 2002: 71 supl. 1: Zakażenia szpitalne: postępy w diagnostyce, terapii i profilaktyce s.19-20, bibliogr. 6 poz. - 9 Zjazd Polskiego Towarzystwa Zakażeń Szpitalnych pt. Zakażenia szpitalne: postępy w diagnostyce, terapii i profilaktyce Poznań 25-27.09. 2002
Sygnatura GBL: 310,004

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

64/2523

Tytuł oryginału: 8th European Congress of Dentomaxillofacial Radiology : programme and abstracts
Źródło: - [Cracow, b.w. 2002], 96 s., 21 cm. - 8 Europejski Kongres Radiologii Stomatologicznej i Szczękowo-Twarzowej Kraków 06-08.06. 2002
Sygnatura GBL: 735,461

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

65/2523

Tytuł oryginału: Immunosupression in liver transplantation for hepatic and biliary malignancy : the effect on tumor recurrence
Autorzy: Pączek Leszek, Ołdakowska-Jedynak Urszula
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[23-27] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

66/2523

Tytuł oryginału: Sixteen-year experience with liver transplantation in hepatocarcinoma, and comparison with liver resection
Autorzy: Gonzalez-Pinto Ignacio
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[28-29] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

67/2523

Tytuł oryginału: Indications and results of liver resection and transplantation for HCC
Autorzy: Jonas Sven, Neuhaus Peter
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[30-33] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

68/2523

Tytuł oryginału: Liver resection and transplantation for primary liver tumors
Autorzy: Farges Olivier
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[33-34] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

69/2523

Tytuł oryginału: Liver resection for HCC : IKEM prague experience
Autorzy: Ryska M., Belina F., Langer D.
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[35] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

70/2523

Tytuł oryginału: Liver transplantation in children with hepatic tumors
Autorzy: Kaliciński Piotr
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[36-37] - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

71/2523

Tytuł oryginału: Surgical treatment of the patients with HCC : experience of the Department of General and Liver Surgery
Autorzy: Krawczyk Marek
Źródło: W: Postępy w chirurgii i transplantacji wątroby : [program, wykłady i streszczenia] - [Warszawa, 2002] s.[38-44], il., tab. - 3 Międzynarodowe Sympozjum pt. Postępy w chirurgii i transplantacji wątroby Warszawa 22-23.11. 2002
Sygnatura GBL: 735,774

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

72/2523

Tytuł oryginału: DOTS Expansion Plan to Stop TB in the WHO European Region 2002-2006
Opracowanie edytorskie: Danzon Marc (przedm.).
Źródło: - [Cophenhagen, World Health Organization 2002], V, 46 s. : il., tab., 30 cm.
Sygnatura GBL: 291,084

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

73/2523

Tytuł oryginału: Relationship between acute and late normal tissue injury after postoperative radiotherapy in endometrial cancer.
Autorzy: Jereczek-Fossa Barbara A., Jassem Jacek, Badzio Andrzej
Źródło: Int. J. Radiat. Oncol. Biol. Phys. 2002: 52 (2) s.476-482, il., tab., bibliogr. 49 poz.
Sygnatura GBL: 312,578

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Purpose: To evaluate the relationship between acute and late normal tissue reaction in 317 consecutive endometrial cancer patients treated with surgery and adjuvant radiotherapy (RT). Methods: The data of 317 patients (staging according to the International Federation of Gynecology and Obstetrics) treated with postoperative RT were analyzed. Both low-dose-rat brachytherapy and external beam RT were applied in 247 patients (78 p.c.); brachytherapy only in 49 (15 p.c.) and external beam irradiation only in 21 (7 p.c.). The median follow-up was 7.3 years (range 4-21). The European Organization for Research and Treatment of Cancer, Radiation Therapy Oncology Group system with elements of the late effects of normal tissue, sibjective, objective, management, analytic (LENT/SOMA) scale was used to score the RT reactions. The correlation between the occurrence and severity of acute and late bowel and bladder toxicity, as well as the relationship between the severity of acute effects and time to occurrence of late reactions, were assessed using linear and logistic regression analyses. Results: Of the 317 patients, 268 (85 p.c.) experienced acute RT reactions of any grade. Severe acute bowel reactions were observed in 15 patients (5 p.c.), urinary bladder complications in 1 patient (0.5 p.c.) cutaneous in 1 patient (0.5 p.c.), and vaginal in 1 patient (0.5 p.c.). Severe acute hematologic toxicity was seen in 3 patients (1 p.c.). A total of 158 patients (51 p.c.) experienced late RT reactions of any grade. Severe late bowel reactions were obsrved in 19 patients (6 p.c.), urinary bladder in 5 (2 p.c.), vaginal in 3 (1 p.c.), and bone in 10 (4 p.c.)...

74/2523

Tytuł oryginału: Detection of enterotoxigenic Clostridium perfringens with a duplex PCR.
Autorzy: Augustynowicz E[wa], Gzyl A., Ślusarczyk J.
Źródło: J. Med. Microbiol. 2002: 51 (2) s.169-172, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 305,350

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Two sets of primers sedigned to detect Clostridium perfringens phospholipase C (plc) and enterotoxin (cpe) genes in a single PCR reaction were applied to a collection of 64 predominantly food poisoning-related C. perfringens isolates. In-vitro enterotoxin synthesis was tested serologically after inducing sporulation. Of the 64 isolates, 26 were clearly enterotoxigenic: 16 were classified as potentially enterotoxigenic only as serological testing did not confirm enterotoxin production, Duplex PCR for diagnosis of enteroxigenic C. perfringens from vegetative cultures can be a useful tool as fresh isolates often sporulate poorly or not all, giving rise to the possibility of false negative results by serological analysis.

75/2523

Tytuł oryginału: Free radicals-mediated induction of oxidized DNA bases and DNA-protein cross-links by nickel chloride.
Autorzy: Woźniak Katarzyna, Błasiak Janusz
Źródło: Mutat. Res 2002: 514 (1/2) s.233-243, il., tab., bibliogr. 50 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Using the comet assay, we showed that nickel chloride at 250-1000 ćM induced DNA damage in human lymphocytes, measured as the change in comet tail moment, which increased with concentration up to 500 ćM and then decreased. Observed increase might follow from the induction of strand breaks or/and alkali-labile sites (ALS) by nickel, whereas decrease from its induction of DNA-DNA and/or DNA-protein cross-links. Proteinase K caused an increase in the tail moment, suggesting that nickel chloride at 1000 ćM might cross-link DNA with nuclear proteins. Lymphocytes exposed to NiCl2 and treated with enzymes recognizing oxidized and alkylate bases: endonuclease III (Endo III), formamidopyrimidine-DNA glycosylase (Fpg) and 3-methyladenine-DNA glycosylase II (AlkA), dusplayed greated extent of DNA damege than those not treated with these enzymes, indicating the induction of oxidized and alkylated bases by nickel. The incubation of lymphocytes with spin traps, 5,5-dimethyl-pyrroline N-oxide (DMPO) and PBN decreased the extent of DNA damage which might follow from the production of free radicals by nickel. The pre-treatment with Vitamin C at 10 ćM and Vitamin E at 25 ćM decreased the tail moment of the cells exposed to CiCl2 at the concentrations of the metal causing strand breaks or/and ALS. The results obtained suggest that free radicals may be involved in the formation of strand breaks or/and ALS in DNA as well as DNA-protein cross-links induced by NiCl2. Nickel chloride can also alkulate DNA bases. Our results support thesis on multiple, free radicals-based genotoxicity pathways of nickel.

76/2523

Tytuł oryginału: Comparative studies of osteoblast and fibroblast type I collagen in a patient with osteogenesis imperfecta type IV.
Autorzy: Galicka Anna, Wołczyński Sławomir, Gińdzieński Andrzej
Źródło: J. Pathol. 2002: 196 (2) s.235-237, il., bibliogr. 16 poz.
Sygnatura GBL: 302,048

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

in vitro

Streszczenie angielskie: The expression of type I collagen has been compared in fibroblast and osteoblast cultures of a patient with moderately severe osteogenesis imperfecta (OI) type IV, with respect to control cells. Electrophoretic analysis of type I collagen showed that both OI osteoblasts and fibroblasts synthesized normal chains with delayed migration. However, the osteoblasts contained a higher proportion of abnormal chains than fibroblasts from the proband. Pulse-chase experiments showed that the trimers containing abnormal chains were cleared more rapidly from osteoblasts than fibroblasts. Moreover, the collagen secreted by OI osteoblests had thermal stability 1řC higher than collagen secreted by OI fibroblasts. These results suggest that the abnormal collagen in osteoblasts may be more resistant to intra- and extracellular degradation and my thus have better survival than in fibroblasts. This finding could have implications for understanding the clinical phenotype of OI.

77/2523

Tytuł oryginału: Potentiating antitumor effects of a combination therapy with lovastatin and butyrate in the Lewis lung carcinoma model in mice.
Autorzy: Giermasz Adam, Makowski Marcin, Kozłowska Ewa, Nowis Dominika, Maj Małgorzata, Jalili Ahmad, Feleszko Wojciech, Wójcik Cezary, Dąbrowska Anna, Jakóbisiak Marek, Gołąb Jakub
Źródło: Int. J. Cancer 2002: 97 (6) s.746-750, il., bibliogr. 32 poz.
Sygnatura GBL: 305,029

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Lovastatin, the drug used for the treatment of hypercholesterolemia, has previously been reported to exert antitumor activity in experimental murine models. Byturate and butyric acid derivatives are well known to induce differentiation and apoptosis of tumour cells and also have recently gained acceptance as potentaial anticancer agents. In this study, we examined the antitumor effects of the combination of lovastatin and butyrate or its prodrug tributyrin in vitro and in vivo against a murine Lewis lung carcinoma (3LL). This combination therapy showed synergistic antitumor activity against 3LL cells in vitro. These effects were at least in part due to apoptosis induction that occurred after 12 hr of incubation with lovastatin and butyrate and was preceded by changes in cell cycle distribution of treated cells and expression of p21, p53 and cyclin D 1. Remarkably, a systemic treatment of syngeneic mice inoculated with 3LL cells with both drugs resulted in significant tumour growth retardation.

78/2523

Tytuł oryginału: Accuracy of transcranial color Doppler ultrasonography in the diagnosis of middle cerebral artery spasm determined by receiver operating characteristic analysis.
Autorzy: Mariak Zenon, Krejza Jarosław, Świercz Mirosław, Kordecki Kazimierz, Lewko Janusz
Źródło: J. Neurosurg. 2002: 96 (2) s.323-330, il., tab., bibliogr. 68 poz.
Sygnatura GBL: 301,435

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Object. The value of transcranial Doppler ultrasonography for the detection of middle cerebral artery (MCA) spasm has been asserted. None of the published studies, however, has adequately scrutinized the overall diagnostic accuracy of this procedure. There are only sporadic reports concerning the utility of transcranial color Doppler (TCCD) ultrasonography, although this method has been proved to be more precise. In this study the authors attempted to estimate the performance of TCD ultrasonography in detecting MCA narrowing by using receiver operating characteristic (ROC) curve analysis, based on TCCD studies obtained in a relatively large, randomly selected population of patients. Methods. Transcranial color Doppler ultrasonography studies were obtained in 100 consecutive patients (54 men and 46 women ages 18-74 years, median age 50 years) routinely referred by neurosurgeons for intraarterial angiography. The M1 segment of the MCA was insonated using a 2.5-MHz probe via a temporal acoustic window, and angle-corrected flow velocities were obtained. Angiographically depicted vasospasm was graded as none, mild (ó 25 p.c. vessel caliber reduction), and moderate to severe ( 25 p.c. vessel caliber reduction). The effectiveness of TCCD ultrasonography in diagnosing MCA spasm was evaluated by calculating the areas under the ROC curves (Az). Of the 200 MCAs examined, 173 were successfully visualized with the aid of TCCD ultrasonography. Mild vasospasm was angiographically diagnosed in 15 arteries and moderate-to-severe vasospasm in 28...

79/2523

Tytuł oryginału: Expression of FasL gene in T cells of renal allograft recipients.
Autorzy: Kasprzycka Monika, Kłodos Konrad, Nowaczyk Maria, Wyzgał Janusz, Podobińska Iwona, Durlik Magdalena, Górski Andrzej
Źródło: Immunol. Lett. 2002: 80 (1) s.9-13, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 312,663

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: FasL molecule expressed on activated T cells induces apoptosis in Fas-expressing cells. It is possible that apoptosis induced by FasL is involved in the process of allograft destruction brought about by infiltrating T cells. The aim of our study was to evaluate expression of FasL gene in peripheral blood T cells of renal allograft recipients (RAR). We have studied 25 patients: 16 with uneventful stable course (RAR-S) and nine during biopsy proven chronic rejection (RAR-CH). The relative expression of FasL mRNA compared with that of á-actin was established by semi-quantitive RT-PCR. We have found that FasL gene expression was significantly increased in T cells of RAR-CH compared to RAR-S (P 0.01). Our results suggest that T cells expression of FasL gene is increased during chronic rejection. Therefore, this phenomenon may pay a role in allograft injury associated with that process. Further studies are needed to unravel possible clinical consequences of observed differences in T cell expression of FasL.

80/2523

Tytuł oryginału: Effects of oestrogen deprivation on interleukin-6 production by peripheral blood mononuclear cells of postmenopausal women.
Autorzy: Rachoń D., Myśliwska J., Suchecka-Rachoń K., Więckiewicz J.
Źródło: J. Endocrinol. 2002: 172 (2) s.387-395, il., tab., bibliogr. s. 393-395
Sygnatura GBL: 310,833

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Various hormones can influence the expression of interleukin-6 (IL-6) and oestrogens are the most extensively studied. There is, however, controversy about the nature of the IL-6 secreted by human cells and its regulation by 17á-oestradiol. The aim of this work was to clarify whether oestrogen deprivation after menopause may contribute to an enhanced IL-6 production by peripheral blood mononuclear cells (PBMC) in postmenopausal women. Twenty-two healthy postmenopausal women, age range 45-63 years with clinical symptoms of oestrogen deficiency were enrolled in the study. The control group consisted of 16 healthy young women, age range 22-31 years, with regular menses and who were not taking oral contraceptives. Levels of IL-6 in the sera and PBMC culture supernatants were measured by the biological B9 cell-proliferation assay and expression of the IL-6 gene in non-stiulated PBMC was detected by RT-PCR. The effect of 17á-oestradiol on spontaneous IL-6 production by the PBMC of postmenopausal women was also studied in vitro and in vivo. Seventeen out of the twenty-two postmenopausal women were given hormonal replacement therapy of 50 ćg 17á-oestradiol/day transdermally and the spontaneous production of IL-6 by the PBMC was analysed after 6 and 12 months of treatment. The postmenopausal women had significantly higher serum levels of IL-6 than the young controls. The spontaneous production of IL-6 by non-stimulated PBMC into the culture supernatants was also significantly higher in the postmenopausal women compared with the young...

81/2523

Tytuł oryginału: Myocardial perfusion correlates with improvement of systolic function of the left ventricle after CABG. Dobutamine echocardiography and Tc-99m-MIBI SPECT study.
Autorzy: Paluszkiewicz L., Kwinecki P., Jemielity M., Dyszkiewicz W[ojciech], Cieśliński A., Szyszko A.
Źródło: Eur. J. Cardiothorac. Surg. 2002: 21 (1) s.32-35, tab., bibliogr. 20 poz. - 14 Doroczne Spotkanie Europejskiego Towarzystwa Chirurgii Klatki Piersiowej i Serca Frankfurt 07-11.10. 2000
Sygnatura GBL: 312,899

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: The aim of the study was to assess the effect of surgical revascularization [coronary artery bypass grafting (CABG)] on systolic function and perfusion of the left ventricle using dobutamine echocardiography (DE) and Tc-99m-MIBI SPECT (SPECT). Methods: There were 32 patients mean age 52.2 ń 7.2 years in whom DE and SPECT were performed before and 3-4 months after CABG using standard protocols. Wall motion score index (WMSI) and perfusion index (PI) were calculated. Results: Significant improvement of WMSI at rest (1.44 ń 0.46 vs 1.33 ń 0.41; P = 0.03) as well as after maximal dose of dobutamine (1.49 ń 0.42 vs 1.30 ń 0.44; P = 0.02) was observed after CABG as compared to preoparative examination. Similar relation was observed during SPECT study. Perfusion index diminished significantly after revascularization during rest acquisition (2.19 ń 0.71 vs 1.93 ń 0.70; P = 0.0008) and after Dipirydamole administration (2.73 ń 0.73 vs 2.20 ń 0.69; P = 0.0001) as compared to preoperative examination. We found correlation between PI and WMSI at rest before CABG (R = 0.46; P = 0.01). PI after Dipirydamole and WMSI after maximal dose of Dobutamine before CABG (R = 0.37; P = 0.04), PI and WMSI at rest CABG (R = 0.39; P = 0.03), PI after Dipirydamole and WMSI after dobutamine after CABG (R = 0.38; P = 0.03). Conclusions: Surgical revascularization significantly improves both perfusion and contractility. Increased perfusion after CABG correlates with improvement of systolic function of the left ventricle.

82/2523

Tytuł oryginału: A new type of mixed anhydride and its applications to the synthesis of 7-substituted 8-chloro-5,5-dioxoimidazo[1,2-b][1,4,2]benzodithiazines with in vitro antitumor activity.
Autorzy: Brzozowski Zdzisław, Sączewski Franciszek
Źródło: J. Med. Chem. 2002: 45 (2) s.430-437, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 312,133

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: A new series of 8-chloro-5,5-dioximidazo[1,2-b][1,4,2]benzodithiazine derivatives 8-25 with heteroaryloxycarbonyl or heteroarylcarbamoyl substituents at position 7 have been synthesized as potential antitumor agents. In this procedure a novel type of mixed anhydride 7 was prepared from 8-chloro-5,5-dioxoimidazo[1,2-b][1,4,2]benzodithiazine-7-carboxylic acid 6 and methanesulfonyl chloride, which in turn was condensed either with heteroarylamines or heteroarylhydroxy compounds. All the compounds prepared were screened at the National Cancer Institute (NCI) for their activities against a panel of 60 tumor cell lines, and relationships between structure and antitumor activity in vitro are discussed. The amides 8, 10, 12, 13, 21, and ester 25 were inactive, whereas the other compounds exhibited rather moderate activity against one or more human tumor cell lines. The prominent compound with remarkable activity (log GI50 -8) and selectivity for the leukemia HL-60(TB) cell line was 2-methyl-8quinolyl 8-chloro-5,5-dioximidazo[1,2-b][1,4,2]benzodithiazine-7-carboxylate 24.

83/2523

Tytuł oryginału: Pulsatile blood pressure component as predictor of mortality in hypertension: a meta-analysis of clinical trial control groups.
Autorzy: Gasowski Jerzy, Fagard Robert H., Staessen Jan A., Grodzicki Tomasz, Pocock Stuart, Boutitie Florent, Gueyffier Francois, Boissel Jean-Pierre
Źródło: J. Hypertens. 2002: 20 (1) s.145-151, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 313,132

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective. Although cirrent guidelines rest exclusively on the measurement of systolic and diastolic blood pressures, the arterial pressure wave is more precisely described as consisting of a pulsatile (pulse pressure) and a steady (mean pressure) component. This study explored the independent roles of pulse pressure and mean pressure as predictors of mortality in a wide range of patients with hypertension. Design and methods. This meta-analysis, based on individual patient data, has combined results from the control groups of seven randomized clinical trials conducted in patients with systolo-diastolic or isolated systolic hypertension. The relative hazard rates associated with pulse pressure and mean pressure were calculated using Cox's proportional hazard regression models with stratification for the seven trials and with adjustment for sex, age, smoking and the other pressure. Results. A 10 mmHg wider pulse pressure at baseline, which corresponds to approximately one-half of its standard deviation, was independently associated with an increase in risk by 6 p.c. for total mortality (P = 0.001), 7 p.c. for cardiovascular mortality (P = 0.01), and 7 p.c. for fatal coronary accidents (P = 0.03). The corresponding increase in risk of fatal stroke was similar (+ 6 p.c., P = 0.27) but there were too few strokes to reach statistical significance. In similar analyses, mean pressure was not identified as an independent predictor of these outcomes. Significant interactions of pulse pressure or mean pressure with age suggested that the prognostic power of pulse pressuer for fatal stroke was more important at higher age (P = 0.04), whereas the prognostic power of mean pressure for coronary mortality was greatest in the young (P = 0.01)...

84/2523

Tytuł oryginału: Inhibition of N-methyl-D-aspartic acid-nitric oxide synthase in rat hippocampal slices by ethanol. Evidence for the involvement of tetrahydrobiopterin but not lipid peroxidation.
Autorzy: Czapski Grzegorz A., Sun Grace Y., Strosznajder Joanna B.
Źródło: J. Biomed. Sci. 2002: 9 (1) s.3-9, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,488

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The ionotropic glutamatergic receptor system, especially the subtype mediated by N-methyl-D-aspartic acid (NMDA), is known to exhibit special sensitivity to the effect of ethanol. This is due partly to the ability of ethanol to modulate the production of nitric oxide through the NMDA-nitric oxide synthase (NOS) pathway. In this study, we examined the effects of ethanol on basal and NMDA-stimulated NOS activity in rat hippocampal slices by measuring the conversion of [**14C]-arginine into [**14C]-citrulline in an incubation system containing the necessary cofactors. Stimulation of hippocampal slices with NMDA (100 ćM) enhanced NOS activity by 43 p.c. (n = 12). Although ethanol did not alter NOS activity when added to the incubation system during NMDA stimulation, it dose-dependently inhibited NMDA-NOS activity when added to the slices during the 90-min preincubation period. Further assay of NOS activity with brain cytosolic fraction indicated an inhibitory effect of ethanol (200 mM) when the assay was carried out in the absence of exogenous tetrahydrobiopterin (BH4), a redox-active cofactor for NOS. Incubation of brain homogenates resulted in a time-dependent increase in the levels of lipid peroxidation products, but ethanol did not further enhance these products. Taken together, these results providence evidence for the role of BH4 but not oxidative stress in the inhibitory effect of ethanol on NMDA-NOS activity in rat hippocampal slices.

85/2523

Tytuł oryginału: Angiogenesis inhibitors specific for methionine aminopeptidase 2 as drugs for malaria and Leishmaniasis.
Autorzy: Zhang Peng, Nicholson Diarmuid E., Bujnicki Janusz M., Su Xinzhuan, Brendle James J., Ferdig Michael, Kyle Dennis E., Milhous Wilbur K., Chiang Peter K.
Źródło: J. Biomed. Sci. 2002: 9 (1) s.34-40, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,488

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Methionine aminopeptidase 2(MetAP2) is responsible for the hydrolysis of the initiator methionine molecule from the majority of newly synthesized proteins. We have cloned the MetAP2 gene from the malaria parasite Plasmodium falciparum (PfMetAP2; GenBank accession number AF348320). The cloned PfMetAP2 has no intron, consists of 1,544 bp and encodes a protein of 354 amino acids with a molecular mass of 40,537 D and an overall base composition of 72.54 p.c. A + T. PfMetAP2 has 40 p.c. sequence identity with human MetAP2 and 45 p.c. identity with yeast MetAP2, and is located in chromosome 14 of P. falciparum. The three-dimensional structure of PfMetAP2 has been modeled based on the crystal structure of human MetAP2, and several amino acid side chains protruding into the binding pocket that differ between the plasmodial and human enzyme have been identified. The specific MetAP2 inhibitors, fumagilin and TNP-470, potently blocked in vitro growth of P. falciparum and Leishmania donovani, with IC50 values similar to the prototype drugs. Furthermore, in the case of P. falciparum, the chloroquine-resistant strains are equally susceptible to these two compounds.

86/2523

Tytuł oryginału: Intrapartum cardiotocography and fetal pulse oximetry in assessing fetal hypoxia.
Autorzy: Leszczyńska-Gorzelak B., Poniedziałek-Czajkowska E., Oleszczuk J.
Źródło: Int. J. Gynecol. Obstet. 2002: 76 (1) s.9-14, tab., bibliogr. 20 poz.
Sygnatura GBL: 312,925

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Objectives: A retrospective analysis of short-term variability (STV), a cardiotocography (CTG) parameter, in relation to fetal blood saturation values (FSpO2) obtained by fetal pulse oximetry. Methods: The study included 26 healthy pregnant women monitored continuously during delivery with both cardiotocography and fetal pulse oximetry. Results: Lower FSpO2 values were observed in the group showing STV levels ó 6.0 ms in the 1st stage of labor (44.7 ń 3.46 p.c. vs. 49.2 ń 1.8 p.c.), but this result was not statistically significant. In the 2nd stage of labor, FSpO2 values in the group with STV levels ó 6.0 ms were significantly lower than those with STV levels 6.0 ms (34.4 ń 2.9 p.c. vs. 43 ń 7.2 p.c.; P 0.001). A positive correlation was found between STV levels ó 6.0 ms and mean FSpO2 values in the 2nd stage of labor. Conclusions: 1. A significant relationship was observed between short-term variability in the cardiotocographic records and fetal blood saturation levels in the 2nd stage of labor. 2. Fetal pulse oximetry can be valuable in assessing fetal wellbeing, especially when CTG records are abnormal.

87/2523

Tytuł oryginału: Apoptosis in the first trimester human placenta: the role in maintaining immune privilege at the maternal-foetal interface and in the trophoblast remodelling.
Autorzy: Jerzak Małgorzata, Bischof Paul
Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 100 (2) s.138-142, il., bibliogr. 56 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Apoptosis has been proposed as a mechanism maintaining immune privilege. Expression of Fas ligand (FasL) by the human trophoblast has been recently accepted as a mechanism providing protection against the lytic action of activated decidual immune cells expressing Fas receptor (FasR). Therefore, the purpose of this review was determine the role of apoptosis in early pregnancy maintenance according to the latest literature. We used Medline literature search. The data suggest that apoptosis may serve as a previously unsuspected mechanism that induces tolerance of the foetal allograft against maternal immune system. Apoptosis of activated maternal immune cells occurs in the human decidua mainly through Fas-FasL or receptor for TNF-related apoptosis-inducing ligand (TRAIL-R)-TNF-related apoptosis-inducing ligand (TRAIL) signalling. This might be a defence mechanism against rejection of the foetal allograft by the maternal immune system. In addition, in this review contribution of programmed cell death to placental cell turnover and remodelling during first trimester of pregnancy is also discussed.

88/2523

Tytuł oryginału: Reduction in fatal pulmonary embolism and venous thrombosis by perioperative administration of low molecular weight heparin. Gynecological ward retrospective analysis.
Autorzy: Cyrkowicz Andrzej
Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 100 (2) s.223-226, tab., bibliogr. 20 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objectives: To compare the frequency of deep vein thrombosis (DVT) and pulmonary embolism (PE) in patients undergoing gynecological operations without low molecular weight heparin (LMWH) prophylaxis and those receiving such prophylaxis. Design: Retrospective, hospital record-based study. Material and methods: About 1785 consecutive patients without LMWH prophylaxis and 1871 ones treated with nadroparin (Fraxiparine) 7500 ICU s.c. 2 h before the operation and repeated daily for 5-7 days or until the patient was fully mobile. DVT was diagnosed on the basis of clinical symptoms and ultrasound examination, and PE clinical symptoms, gasometric data, electrocardiography and chest X-ray. Results: Among the patients without prophylaxis, four cases of PE occurred (0.22 p.c.), two fatal (0.11 p.c.), 13 cases of proximal DVT (0.72 p.c.) and 41 distal DVT complications (2.3 p.c.). In LMWH, group 3 proximal DVT (0.16 p.c.) and 18 distal DVT (0.96 p.c.) developed but there were no clinically expressed pulmonary embolism. According to the Fisher's exact test, the difference between the complications in the analyzed groups is significant P 0.05. Conclusions: The perioperative applying of LMWH to prevent DVT in the patients operated on gynecologically is effective.

89/2523

Tytuł oryginału: Immunopharmacological potential of selective phosphodiesterase inhibition.[P.] 1: Differential regulation of lipopolysaccharide-mediated proinflammatory cytokine (interleukin-6 and tumor necrosis factor-ŕ) biosynthesis in alveolar epithelial cells.
Autorzy: Haddad John J., Land Stephen C., Tarnow-Mordi William O., Zembala Marek, Kowalczyk Danuta, Lauterbach Ryszard
Źródło: J. Pharmacol. Exp. Ther. 2002: 300 (2) s.559-566, il., bibliogr. s. 565-566
Sygnatura GBL: 310,547

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In an attempt to elaborate in vitro on a therapeutic strategy that countracts an inflammatory signal, we previously reported a novel immunopharmacological potential of glutathione, an antioxidant thiol, in regulating inflammatory cytokines. In the present study, we investigated the hypothesis that selective regulation of phosphodiesterases (PDEs), a family of enzymes that controls intracellular cAMP/cGMP degradation, differentially regulates proinflammatory cytokines. Selective PDE1 inhibition (8-methoxymethyl-3-isobutyl-1-methylxanthine) blockaded lipopolysaccharide-endotoxin (LPS)-mediated biosynthesis of interleukin (IL)-6, but this pathway had no inhibitory effect on tumor necrosis factor-ŕ (TNF-ŕ). Furthermore, inhibition of PDE3 (amrinone) abolished the effect of LPS on IL-6, but attenuated TNF-ŕ production. Reversible competitive inhibition of PDE4 (rolipram) exhibited a potent inhibitory effect on IL-6 and a dual, biphasic (excitatory/inhibitory) effect on TNF-ŕ secretion. Blockading PDE5 (4-[3',4'-(methylenediox)benzyl]amino-6-methoxyquinazoline) showed a high potency in reducing IL-6 production, but in a manner similar to the inhibition of PDE4, exhibited a biphasic effect on TNF-ŕ biosynthesis. Simultaneous inhibition of PDE5, 6 and 9 (zaprinast), purported to specifically elevate intracellular cGMP, reduced, in a doseindependent manner, IL-6 and TNF-ŕ biosynthesis. Finally, nonselective inhibition of PDE by pentoxyfylline suppressed LPS-mediated secretion of IL-6 and TNF-ŕ. The involvement of specific PDE isoenzymes in differentially regulating LPS-mediated inflammatory cytokine biosynthesis indicates a novel approach to unravel the potential therapeutic targets that these isozymes constitute during the progression of inflammation within the respiratory epithelium.

90/2523

Tytuł oryginału: Immunopharmacological potential of selective phosphodiesterase inhibition. [P.] 2: Evidence for the involvement of an inhibitory-kB/nuclear factor-kB-sensitive pathway in alveolar epithelial cells.
Autorzy: Haddad John J., Land Stephen C., Tarnow-Mordi William O., Zembala Marek, Kowalczyk Danuta, Lauterbach Ryszard
Źródło: J. Pharmacol. Exp. Ther. 2002: 300 (2) s.567-576, il., tab., bibliogr. s. 575-576
Sygnatura GBL: 310,547

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: In this report we investigated the immunopharmacological role of selective and nonselective phosphodiesterase (PDE) inhibition in regulating the inhibitory-kB (IkB-ŕ)/nuclear factor-kB (NF-kB) signally transduction pathway. In fetal alveolar type II epithelial cells, PDE blockade at the level of the diverging cAMP/cGMP pathways differentially regulated the phosphorylation and degradation of IkB-ŕ, the major cytosolic inhibitor of NF-kB. Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kB1 (p50), RelA (p65), RelB (p68), and c-Rel (p75), selective blockade of PDE 5, 6, and 9, by the action of 4-[3',4'=(methylenedioxy)benzyl]amino)-6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS)-mediated NF-kB translocation. Pentoxifilline, a nonspecific PDE inhibitor, reversed the excitatory effect of LPS on NF-kB subunit nuclear localization, in a dosedependent manner. Furthermore, analysis of NF-kB activation under the same conditions revealed a biphasic effect mediated by LPS. PDEs 1, 3, and 4 inhibition was associated with upregulating NF-kB transcriptional activity. In contrast, blockading the activity of PDEs 5, 6, and 9 negatively attenuated LPS-mediated NF-kB activation, similar to the effect of 3,7-dihydro-3,7-dimethyl-1-(5-oxohexyl)-1H-purine-2,6-dione (pentoxifulline). These results indicate that selective and nonselective interference with the control of the dynamic equilibrium of cyclic nucleotides via PDE isoenzyme regulation represents an immunoregulatory mechanism that requires the differential, biphasic targeting of the IkB-ŕ/NF-kB pathway.

91/2523

Tytuł oryginału: Matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1: a possible role in the pathogenesis of endometriosis.
Autorzy: Szamatowicz J., Laudański P., Tomaszewska I.
Źródło: Hum. Reprod. 2002: 17 (2) s.284-288, il., tab., bibliogr. s. 287-288
Sygnatura GBL: 313,265

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Background: Matrix metalloproteinases (MMPs) are a family of endopeptidases which play a role in the degradation and turnover of extracellular matrix proteins. Their action is regulated by specific tissue inhibitors called tissue inhibitors of metalloproteinases (TIMPs). Methods: We measured the concentrations of total and active MMP-9 in peritoneal fluid of infertile women with mild or moderate endometriosis (n = 22) and compared them with those in a control group of infertile patients (n = 21). Results: We found that the mean (ń SD) total concentrations of MMP-9 in the peritoneal fluid of patients with endometriosis was 6.2 ń 1.8 ng/ml, in comparison with 2.9 ń 2.6 ng/ml in the control group (P = 0.001). Concenrations of active MMP-9 did not differ significantly between the groups. The concentrations of TIMP-1, after logarithmic transformation, were significantly lower (P = 0.017) in endometriotic peritoneal fluids than in peritoneal fluid of control women, 1.02 ń 0.21 ng/ml and 1.16 ń 0.18 ng/ml respectively. No correlation between stage of disease, steroid hormone concentration, MMP-9 (total and active) and TIMP-1 was found. Conclusions: These results suggest that a disturbed equilibrium exists between MMP-9 and TIMP-1 in peritoneal fluid of women with endometriosis. This may play an important role in the pathogenesis of the disease.

92/2523

Tytuł oryginału: HA 1004, an inhibitor of serine/threonine protein kinases, restores the sensitivity of thymic lymphomas to Caý+ -mediated apoptosis through a protein kinase A-independent mechanism.
Autorzy: Matuszyk Janusz, Cebrat Małgorzata, Kalas Wojciech, Strzadała Leon
Źródło: Int. Immunopharmacol. 2002: 2 (4) s.435-442, il., bibliogr. 20 poz.
Sygnatura GBL: 313,577

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Our revious reports showed that thymic lymphomas arising in TCR transgenic mice are resistant to Caý+ -mediated apoptosis. Here we show that induction of apoptosis in thymic lymphomas involves a process that is cAMP-mediated and which depends on the activation of protein kinase A (PKA) despite the lower level of PKA type I in these lymphomas compared to thymocytes. Further, we show that treatment of the lymphomas with HA1004, a serine/threonine protein kinase inhibitor, restores their susceptibility to ionomycin-induced apoptosis. Results indicate that HA1004-induced restoration of sensitivity to ionomycin proceeds through a PKA-independent mechanism. Moreover, activation of PKA instead of its inhibition induces apoptosis of lymphoma cells.

93/2523

Tytuł oryginału: Lactoferrin immunomodulation of DTH response in mice.
Autorzy: Actor Jeffrey K., Hwang Shen-An, Olsen Margaret, Zimecki Michał, Hunter jr Robert L., Kruzel Marian L.
Źródło: Int. Immunopharmacol. 2002: 2 (4) s.475-486, il., tab., bibliogr. 51 poz.
Sygnatura GBL: 313,577

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Improved nontoxic adjuvants, especially adjuvants capable of inducing cell-mediated immunity (CMI), are needed for research in immunology and for development of human and veterinary vaccines. Bovine Lactoferrin, an effector molecule shown to directly participate in host defense, was assessed at various concentrations as an adjuvant component for induction of DTH responses to sheep red blood cells (SRBC). Subcutaneous immunization with Lactoferrin enhanced delayed type hypersensitivity (DTH) in CBA mice in a dose-dependent fashion; DTH responses were most significantly increased when sensitization was accomplished using Lactoferrin at 50 ćg/dose and 250 ćg/dose. Furthermore, Lactoferrin admixed with suboptimal dose of SRBC enhanced DTH responses by over 17-fold. Peritoneal cells collected from mice intraperitoneally injected with a 100 ćg/dose of Lactoferrin demonstrated modest, but significant, production of TNF-ŕ, IL-12 and MIP-1ŕ, when cultured in vitro, compared to saline-injected controls. J774A.1 murine macrophages stimulated with Lactoferrin resulted in increased TNF-ŕ protein production, and upregulated IL-12 and IL-15 mRNA. Levels of message for chemokins MIP-1ŕ and MIP-2 were also increased in a dose-dependent way. Taken together, these results indicate that Lactoferrin as an adjuvant may stimulate macrophages to generate a local environment likely to push immune responses towards development and maintenance of CMI.

94/2523

Tytuł oryginału: Nitric oxide and convulsions in 4-aminopyridine-treated mice.
Autorzy: Tutka Piotr, Młynarczyk Małgorzata, Żółkowska Dorota, Kleinrok Zdzisław, Wielosz Marian, Czuczwar Stanisław J.
Źródło: Eur. J. Pharmacol. 2002: 437 (1/2) s.47-53, il., tab., bibliogr. s. 52-53
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: We studied whether N**G-nitro-L-arginine (NNA), an inhibitor of nitric oxide (NO) synthase as well as L-arginine and molsidomine, two agents elevating NO, influenced convulsions caused by 4-aminopyridine, a K+ channel blocker in mice. NNA, in a dose known to decrease level of NO (40 mg kg -**1), enhanced the susceptibility to intraperitoneal (i.p.) and intracerebroventricular (i,c,v,) 4-aminopyride. L-arginine (500 mg kg -**1) and molsidomine (20 mg kg -**1) alone did not influence 4-aminopyridine-induced seizure activity. Surprisingly, the proconvulsant effect of NNA upon clonic and tonic seizures was potentiated by molsidomine (20 mg kg -**1). No influence of L-arginine on the proconvulsant effect of NNA was found. Taking into account the proconvulsant effect of NNA, an involvement of NO-mediated events in the mechanism of convulsive activity of 4-aminopyridine might by postulated, However, the ineffectiveness of L-arginine and molsidomine to suppress the convulsive activity of 4-aminopyridine as well as a paradoxical potentiation of the proconvulsant effect of NNA by molsidomine seem to exclude the impact of NO pathway on 4-aminopyridine-induced convulsions in mice. Our data suggest that the proconvulsant effect of NNA in this seizure model is caused by other, not related to NO, mechanisms.

95/2523

Tytuł oryginału: Decortication in chronic pleural empyema - effect on lung function.
Autorzy: Rzyman Witold, Skokowski Jan, Romanowicz Grzegorz, Lass Piotr, Dziadziuszko Rafał
Źródło: Eur. J. Cardiothorac. Surg. 2002: 21 (3) s.502-507, il., bibliogr. 24 poz.
Sygnatura GBL: 312,899

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: The aim of the present study is to evaluate the lung function before and after the lung decortication in patients with chronic pleural empyema (CPE).Methods: Twenty-six patients with diagnosis of CPE were evaluated in a prospective manner by lung perfusion scintigraphy, blood gas analysis and spirometry before and 35 weeks (ń 17) after the lung decortication. Results: Preoperative scintigraphy showed reduction of lung perfusion on the affected side to 24.5 p.c. (ń 12.6 p.c.) in 11 right side empyemas (predicted value 55 p.c.) and to 18 p.c. (ń 8 p.c.) in 15 left side empyemas (predicted value 45 p.c.). The postoperative measurements showed improvement in perfusion to 45.2 p.c. (ń 7.7 p.c.) in patients with right side empyema and 34.1 p.c. (ń 8.5 p.c.) with the left side affection. The preoperative vital capacity (VC) was reduced to 62.3 p.c. (ń 13.8 p.c.) of the predicted value and forced expiratory volume in 1 s (FEV1) to 50 p.c. (ń 15.5 p.c.) of the predicted value. Postoperatively, slight improvement was achieved to 79.8 p.c. (ń 12.9 p.c.) for VC and 69.2 p.c. (ń 12.7 p.c.) for FEV1. Blood gas analysis showed decreased value in majority of the patients before operation and significant improvement in postoperative evaluation: Conclusion: Perfusion and spirometry improves significantly in patients with CPE after the lung decortication but function of the affected lung remains impaired. There was no influence of the age, gender, side of the disease, bacteriology or duration of the empyema before operation on lung function.

96/2523

Tytuł oryginału: 2- Chloroadenosine, a preferential agonist of adenosine A1 receptors, enhances the anticonvulsant activity of carbamazepine and clonazepam in mice.
Autorzy: Borowicz Kinga K., Łuszczki Jarogniew, Czuczwar Stanisław J.
Źródło: Eur. Neuropsychopharmacol. 2002: 12 (2) s.173-179, il., tab., bibliogr. [28] poz.
Sygnatura GBL: 313,361

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: 2-Chloroadenosine (0.25 - 1 mg/kg) significantly raised the threshold for electroconvulsions in mice. This preferential adenosine A1 receptor agonist (at 0.125 mg/kg) significantly potentiated the protective activity of carbamazepine against maximal electroshock-induced seizures in mice. 2-Chloroadenosine (1 mg/kg) showed also anticonvulsive efficacy against pentylenetetrazol-evoked seizures, raising the CD50 vale for pentylenetetrazol from 77.2 to 93.7 mg/kg. The drug (at 0.5 mg/kg) significantly enhanced the protective action of clonazepam in this test, decreasing its ED50 value from 0.033 to 0.011 mg/kg. Moreover, aminophylline, a non-selective adenosine receptor antagonist (5 mg/kg), and 8-cyclopentyl-1,3-dimethylxanthine (8-CPX), a selective A1, adenosine receptor antagonist (5 mg/kg) reversed the 2-chloroadenosine (0.125 mg/kg)-induced enhancement of the protective activity of carbamazepine and clonazepam. 2-Chloroadenosine administered alone or combined with antiepileptic drugs, caused neither motor nor long-term memory impairment. Finally, the adenosine A1 agonist did not change the free plasma concentration of antiepileptic, so a pharmacokinetic factor is not probable. Summing up, 2-chloroadenosine potentiated the protective activity of both carbamazepine and clonazepam, which seems to be associated with the enhancement of purinergic transmission mediated through adenosine A1 receptors.

97/2523

Tytuł oryginału: The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines. P. 1: Reduction of cytostatic effects of quinacrine mustard by caffeine.
Autorzy: Kapuściński Jan, Ardelt Barbara, Piosik Jacek, Zdunek Małgorzata, Darzynkiewicz Zbigniew
Źródło: Biochem. Pharmacol. 2002: 63 (4) s.625-634, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Recently, accumulated statistical data indicate the protective effect of caffeine consumption against several types of cancer diseases. There are also reports about protective effect of caffeine and other xanthines against tumors induced by polycyclic aromatic hydrocarbons. One of the explanations is based on biological activation of such carcinogens by cytochromes that are also known for metabolism of caffeine. However, there is also numerous data indicating reverse effect on cytotoxicity of anticancer drugs that inhibit the action of topoisomerase I (e.g. Camptothecin or Topotecan) and topoisomerase II inhibitors (e.g. Doxorubicin, Mitoxantrone or mAMSA). In this work we tested the hypothesis that the caffeine protective effect is the result of sequestering of aromatic mutagens by formation of stacking (ă - ă) complex. As the models for the study we have chosen two well-known mutagens, that do not require metabolic activation: quinacrine mustard (QM, aromatic, heterocyclic nitrogen mustard) and mechlorethamine (NM2, aliphatic nitrogen mustard). The flow cytometry study of these agents' action on the cell cycle of HL-60 cells indicated that caffeine prevents the cytotoxic action of QM, but not that of NM2. The formations of stacking complexes of QM with caffeine were confirmed by light absorption, calorimetric measurements and by molecular modeling calculation. Using the statistical thermodynamics calculations we calculated the "neighborhood" association constant (KAC = 59 ń 2 M**-1) and enthalpy change (DeltaH**0' = -116 cal mol**-1); the favorable entropy change of complex formation (DeltaS**0' = 7.72 cal mol**-1 K**-1, ...

98/2523

Tytuł oryginału: The modulation by xanthines of the DNA-damaging effect of polycyclic aromatic agents. P. 2: The stacking complexes of caffeine with doxorubicin and mitoxantrone.
Autorzy: Piosik Jacek, Zdunek Małgorzata, Kapuściński Jan
Źródło: Biochem. Pharmacol. 2002: 63 (4) s.635-646, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Recently accumulated statistical data indicate the protective effects of caffeine consumption against several types of cancer diseases. There are also reports, about protective effect of caffeine and other xanthines against tumors induced by polycyclic aromatic hydrocarbons. One of the explanations of this phenomenon is based on biological activation of such carcinogens by cytochromes that are also known for metabolism of caffeine. In the accompanying paper [Kapuścinski et al., this issue] we provide evidence (flow cytometry and the cell cycle analysis) that the cytostatic effects of caffeine (CAF) on two DNA alkylating agents, which do not require the biological activation, depend on their ability to form stacking (ă - ă) complex. In this study, we use physicochemical techniques (computer aided light absorption and microcalorimetry), and molecular modeling to examine previously published qualitative data. This is published both by our and other group's data, indicates that CAF is able to modify the cytotoxic and/or cytostatic action of the two well known antitumor drugs doxorubicin (DOX) and mitoxantrone (MIT). To obtain the quantitative results from the experimental data we used the statistical-thermodynamical model of mixed aggregation, to find the association constants KAC of the CAF-drug interaction (128 ń 10 and 356 ń 21 M**-1 for DOX-CAF and MIT-CAF complex formation, respectively). In addition, the favorable enthalpy change of CAF-MIT (DeltaH = -11.3 kcal/mol) was measured by microcalorimetry titration. The molecular modeling (semi-empirical and force field method)...

99/2523

Tytuł oryginału: Assessment of the quality and frequency of mutations occurrence in PTEN gene in endometrial carcinomas and hyperplasias.
Autorzy: Konopka Bożena, Paszko Zygmunt, Janiec-Jankowska Aneta, Goluda Marian
Źródło: Cancer Lett. 2002: 178 (1) s.43-51, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 306,023

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The quality and frequency of mutations in PTEN gene were assessed in 59 carcinomas and 6 hyperplasias of the endometrium in women. Screening for mutations was done in all exons of PTEN gene by the PCR-SSCP analysis and DNA sequencing. Results were correlated with histological status and clinical features of endometrial carcinomas. In 45.8 p.c. (27/59) of carcinomas, 36 somatic mutations were detected in PTEN gene. In seven carcinomas, two mutations and in one carcinoma there mutations coexisted simultaneously. Moreover in 33.3 p.c. (2/6) of hyperplasia cases mutations were shown. Most identified mutations (57.9 p.c.) were present in exons 5 and 8, less frequently in exons 2 (15.8 p.c.) and 7 (13.2 p.c.) and they were least frequent in exons 1 and 3 (5.3 p.c. each). No mutations were found in exons 4, 6 and 9. Of all identified mutations, 73.7 p.c. of these resulting in truncated protein were present due to deletions, insertions and nonsense mutations. Missense mutations accounted for 13.2 p.c. of mutations and they were present only in exon 5. One point mutation (2.5 p.c.) was in intronic splice site. The remaining 10.5 p.c.of mutations were neutral polymorphisms. No statistically significant correlation were found between the frequency of PTEN gene mutations and the clinical stage of endometrial carcinomas. However, evident statistically significant, reverse correlation were observed between the frequency of mutations and the grade of morphological differentation of the diseases (Xý = 7.2393, ŕ = 0.0071). In conclusion, our data support the view that PTEN gene mutations are frequent events involved in development of endometrial carcinomas in women.

100/2523

Tytuł oryginału: Accuracy of linear endoscopic ultrasonography in the evaluation of patients with suspected common bile duct stones.
Autorzy: Kohut M., Nowakowska-Duława E., Marek T., Kaczor R., Nowak A.
Źródło: Endoscopy 2002: 34 (4) s.299-303, tab., bibliogr. 28 poz.
Sygnatura GBL: 305,439

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background and study aims: Endoscopic ultrasonography (EUS) with radial scanning is an efficient diagnostic tool where there is suspicion of common bile duct (CBD) stones. Little is known about the use of linear EUS in this condition. The aim of this study was to evaluate the diagnostic efficiency of linear EUS in a large group of patients suspected to have bile duct stones, using endoscopic retrograde cholangiopancreatography (ERCP) with endoscopic sphincterotomy and exploration of the CBD using a Dormia basket, or surgical choledochotomy with choledochoscopy, as diagnostic "gold standards". Patients and methods: 134 patients with clinical suspecion of CBD stones were included in the study and prospectively evaluated, using EUS, and ERCP with endoscopic sphincteroctomy (127 patients), or choledochotomy with choledochoscopy where ERCP was unsuccessful (seven patients). EUS was done before ERCP using an echo endoscope (Pentax FG 32 UA; 5 - 7.5 MHz) and Hitachi EUB 405 ultrasound machine. ERCP was done using the TFJ 100 or TJ 20 Olympus duodenoscope. ERCP was carried out within a mean of 2 days after EUS. The longest time between EUS and ERCP was 3 days. The examiners were binded to the results of the other method used. Results: CBD stones were found in 91 (68 p.c.) patients at ERCP with ES or at surgery. The correct diagnosis was established by EUS in 85 patients. The remaining 43 patients without CBD stones were correctly diagnosed in 41 cases by means of EUS, giving an accuracy of 94 p.c., specificity of 93 p.c., a positive predictive value of 98 p.c., a negative predictive value of 87 p.c., and a Youden's index of 98 p.c....

101/2523

Tytuł oryginału: The respriation of mitochondria from human skeletal muscle coupled to ATP synthesis is not impaired in pH 6.45 medium but is depressed at pH 7.8.
Tytuł polski: Oddychanie mitochondriów mięśnia szkieletowego człowieka skojarzone z syntezą ATP - bez zmian w środowisku o pH 6,45, hamowanie w pH 7,8.
Autorzy: Popinigis Jerzy, Antosiewicz Jędrzej, Crimi Massimo, Kaczor Jan Jacek, Bargossi Alberto Mario, Mercuri Mario, Gruca Zbigniew, Lenaz Giorgio
Źródło: Med. Sport. 2002: 18 (1) s.11-24, il., tab., bibliogr. 40 poz., streszcz.
Sygnatura GBL: 313,155

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Cel pracy: W czasie pierwszych kilku sekund wysiłku fizycznego w wyniku działania enzymu kinazy kreatynowej, wnętrze komórki mięśniowej ulega alkalizacji - wg Conetta nawet do pH 7,8. W wyniku kontynuacji wysiłku i powstawania kwasu mlekowego dochodzi następnie do zakwaszenia cytosolu, a obniżenie pH do wartości 6,45 doprowadza do zatrzymania glikolizy. Celem niniejszej pracy było zbadanie jak zmiany pH środowiska z wartości 7,0 do 6,45 i z 7,0 do 7,8 będą wpływały na aktywność oddechową mitochondriów otrzymanych z mięśnia szkieletowego człowieka. Materiały i metody: mitochondria izolowano z mięśni szkieletowych pochodzących z zabiegów operacyjnych typu amputacji dokonywanych ze wskazań lekarskich. Pomiaru zużycia tlenu przez mitochondria dokonywano za pomocą klasycznej elektrody tlenowej Clarka. Stosowano środowiska o pH 7,0 lub 6,45, lub 7,8. Substratami były: glutaminian z jabłczanem, pirogronian z jabłczanem oraz mleczan z NAD. Wyniki: w przypadku oddychania wysoko sprzężonego z syntezą ATP krótka ekspozycja mitochondriów do warunków pH 6.45 nei zmieniała ich aktywności oddechowej. Wyjątkiem było utlenianie mlecznu z NAD, które ulegało zahamowaniu. Mitochondria, których oddychanie było słabiej skojarzone z syntezą ATP, nieznacznie zmniejszały zużycie tlenu w pH 6.45. Natomiast 50 proc. hamowania obserwowano w pH 6.45 w mitochondriach z uszkodzoną strukturą. Hamowanie oddychania obserwowano w pH 7.8, nawet w mitochondriach dobrze skojarzonych. Obniżenie pH z 7,0 ...

Streszczenie angielskie: Aim of the study: during the first seconds of exercise due to creatine kinase reaction the interior of the muscle cell becomes alkaline, and according to Connett reaches pH 7.8 When exercise continues, the cytosol - due to formation of lactic acid - becomes acidic and glycolysis stops when pH reaches value of 6.45. The aim of our research was to investigate how changes of the pH of the medium from 7,0 to 6.45 or from 7.0 to 7.8 would affect the respiratory activity of mitochondria from human skeletal muscle. Materials and methods: mitochondria were isolated from post amputationem muscles of patients undergoing surgery. Oxygen uptake was measured with the classical Clark type oxygen electorde in pH 7.0, pH 6.45 and pH 7.8 media. Glutamate + malate, or pyruvate + malate, or NAD + lactate were used as respiratory substrates. Results: The respiration that was tightly coupled to ATP synthesis was found not impaired by short exposure to medium with pH 6.45. Exception was lactate (+ NAD) oxidation, which was arrested at this pH. Slight inhibition of respiration at pH 6.45 was noted in loose-coupled mitochondria, but almost 50 p.c. inhibition was seen at pH 6.45 in disrupted mitochondria. The coupled respiration of mitochondria was found inhibited at pH 7.8. Lowering of pH from 7.0 to 6.0 did not affect the activity of complex IV of respiratory chain, but complex I was under these conditions inhibited 39 p.c. and complex III 53 p.c. Activity of complex IV was inhibited 36 ...

102/2523

Tytuł oryginału: The search for optimal treatment in relapsed and refractory acute myeloid leukemia.
Autorzy: Robak Tadeusz, Wrzesień-Kuś Agata
Źródło: Leuk. Lymphoma 2002: 43 (2) s.281-291, tab., bibliogr. 116 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Despite the significant progress in the treatment of AML during the last 5-10 years, 20-40 p.c. of patients still do not achieve remission with standard induction therapy. In addition, 50-70 p.c. of patients in CR are likely to relapse. A major limitation of successful AML therapy is intrinsic or acquired drug resistance. Several pharmacological inhibitors of mechanisms inducing chemoresistance in leukemic cells have been investigated. New cytotoxic drugs, agents with novel mechanisms of action, and new treatment strategies are currently being investigated. The management of refractory or relapsed AML patients is reviewed in this study.

103/2523

Tytuł oryginału: In vitro drug resistance profiles of adult acute lymphoblastic leukemia: possible explanation for difference in outcome to similar therapeutic regimens.
Autorzy: Styczyński Jan, Wysocki Mariusz
Źródło: Leuk. Lymphoma 2002: 43 (2) s.301-307, il., tab., bibliogr. 79 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Age has important prognostic impact in acute lymphoblastic leukemia (ALL). Adults with ALL have a worse prognosis compared to children. This may be due to different, unfavorable biology, poor treatment tolerance, drug resistance, higher expression of drug resistance related proteins. The lymphoblasts from adult ALL show an increased in vitro resistance to cytotoxic drugs, including prednisolone, dexamethasone, cytosine arabinoside, daunorubicin, L-asparaginase and methotrexate. Glucocorticoid resistance may be a fundamental difference between children, adolescents and adults with ALL, which may underlie different biological aspects and also explain the difference in prognosis. It seems that in vitro resistance to prednisolone with respect to the age might be a continuous variable in ALL patients, except infants. The greater the age, the higher the in vitro resistance to prednisolone. This may be due to induction of various defense mechanisms, such as an activation of P-glycoprotien, which develops throughout the life and protect the human against xenobiotics. Among a number of various drug resistance mechanisms, only several weak differences between adults and children with ALL have been reported including higher P-glycoprotein expression, lower methotrexate polyglutamate accumulation and possibly more often p53 gene mutations in adults. Intrinsic resistance, induction of drug resistance proteins expression during chemotherapy and co-existence of various mechanisms are common phenomena in adult ALL. It seems that age itself, more than drug resitance profile, reflects factors which have direct effect on chemotherapy response in adult ALL.

104/2523

Tytuł oryginału: G-CSF administered in time-sequenced setting during remission induction and consolidation therapy of adult acute lymphoblastic leukemia has beneficial influence on early recovery and possibly improves long-term outcome: a randomized multicenter study.
Autorzy: Hołowiecki Jerzy, Giebel Sebastian, Krzemień Sławomira, Krawczyk-Kuliś Małgorzata, Jagoda Krystyna, Kopera Małgorzata, Hołowiecka Beata, Grosicki Sebastian, Hellmann Andrzej, Dmoszyńska Anna, Paluszewska Monika, Robak Tadeusz, Konopka Lech, Maj Stanisław, Wojnar Jerzy, Wojciechowska Maria, Skotnicki Aleksander, Baran Wojciech, Cioch Maria
Źródło: Leuk. Lymphoma 2002: 43 (2) s.315-325, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

ludzie

Streszczenie angielskie: Sixty-four untreated adult acute lymphoblastic leukemia (ALL) patients were randomized to receive chemotherapy alone, n = 31 or chemotherapy and granulocyte colony stimulating factor (G-CSF), n = 33. During induction patients received G-CSF for 5 days between four weekly Epirubicin + Vcradministrations, starting 36 h after each application and finishing 48 h before the next one with the intention to possibly generate a cell cycle dependent protection of normal hematopoietic progenitors and to stimulate granulopoiesis. The complete remission (CR) rate equaled 94 p.c. in the G-CSF group and 87 p.c in controls. Patients who received G-CSF, if compared to the controls, had shorter granulocytopenia during induction and consolidation, displayed a lower infection rate, completed the induction-consolidation quicker and stayed shorter in hospital during induction, p 0.001 - 0.04. Follow-up at 2 years revealed a rather higher probability of survival (59 vs. 27 p.c., p = 0.04) and a lower relapse rate (32 vs. 60 p.c.) in G-CSF arm than in controls. The beneficial influence of G-CSF administered in time-sequenced fashion on survival needs further confirmation.

105/2523

Tytuł oryginału: Fludaraibine therapy in Waldenstrm's macroglobulinemia patients treated previously with 2-chlorodeoxyadenosine.
Autorzy: Lewandowski Krzysztof, Halaburda Kazimierz, Hellmann Andrzej
Źródło: Leuk. Lymphoma 2002: 43 (2) s.361-363, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Six patients with Waldenstrm's Macroglobulinemia (WM) resistant to previous 2-chlorodeoxyadenosine (2-CdA) therapy were treated with fludarabine. Both initial and subsequent therapy was administered according to the most widely used protocols. The median number of 2-CdA cycles given to the patients was 3.7 (range 2-5), while fludarabine was 1.7 (range 1-2). As a result partial remission was achieved in two patients and stabilization of the disease in the remaining four in spite of the inadequate response or progressive disease after 2-CdA therapy. Median monoclonal IgM concentration fell from 29.4 (range 11.6-37.1) to 16.4 g/l (range 3.8-29.1). At the same time hemoglobin concentration increased from median 114 (range 89-129) to 122 g/l (range 97-133). Side effects consisted of neutropenia (three patients) and thrombocytopenia (two patients). Two patients are alive with progression of the disease noted after 12 and 21 months of observation. One patients died as the result of cardiac disease, two other died of infection 5 and 16 months after cessation of therapy in the course of disease progression. The remaining patient was lost to follow-up after 10 months of observation with continuous stabilization. Our observation suggest that in WM patients resistant to 2-CdA, stabilization of the disease or even remission can be obtained with fludarabine.

106/2523

Tytuł oryginału: Production of proangiogenic cytokines during thalidomide treatment of multiple myeloma.
Autorzy: Dmoszyńska Anna, Bojarska-Junak Agnieszka, Domański Damian, Roliński Jacek, Hus Marek, Soroka-Wojtaszko Maria
Źródło: Leuk. Lymphoma 2002: 43 (2) s.401-406, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Recently a growing number of studies have suggested the efficacy of thalidomide (THAL) in the treatment of relapsed or resistant multiple myeloma. Some of these studies indicate that the thalidomide antimyeloma effect is associated with decreased vessel density. Here we first present our experience with THAL treatment and then focus on the determination of the role of proangiogenic cytokines during THAL therapy. Thirty relapsing or resistant multiple myeloma (MM) patients were treated with THAL at a median dose of 400 mg/daily. Eighteen responded to THAL therapy and 12 were resistant or intolerant to THAL. We determined the plasma level of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) as the main biological parameters associated with tumour angiogenesis. In addition I1-6 and TNFŕ levels were also assayed. Assessment of peripheral blood (PB) and bone marrow (BM) cytokine levels were done before and during THAL treatment at weeks 4 and 8 of therapy. In the responder group VEGF, bFGF I1-6 and TNFŕ concentrations were significantly decreased after four weeks of therapy both in PB and BM. In the non-responder group no significant changes in bFGF and VEGF levels were observed. However, a significant increase in IL-6 and TNF concentrations was evident. We conclude that the significant decrease of VEGF, bFGF, I1-6 and TNFŕ concentrations reflected response to THAL therapy. Also it seems that VEGF is a better marker of response to treatment than bFGF.

107/2523

Tytuł oryginału: Influence of the alpha-1-adrenergic receptor blocker doxazosin on exercise-induced hyperkalemia in hemodialysis patients.
Autorzy: Nowicki Michał, Waluś Tomasz, Kokot Franciszek
Źródło: Kidney Blood Press. Res. 2002: 25 (1) s.55-60, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 313,314

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background/Aim: Hyperkalemic responses to both physical exercise and ŕ-adreenergic stimulation are enhanced in patients with terminal renal failure. ŕ-Adrenergic blockade was found to protect against hyperkalemia during vigorous exercise in healthy men. The aim of the study was to examine the effects of the ŕ-adrenergic blocker doxazosin on exercise-induced hyperkalemia in hemodialysis patients. Methods: In a randomized, placebo-controlled, crossover design study, 15 anuric, chronic hemodialysis patietns were included. Doxazosin or placebo was given in a random order for 4 days before exercise. At the end of each phase of the study, a 30-min treadmill exercise test with a constant workload of 2 metabolic equivalents was performed followed by a 30-min recovery period. Results: The patients achieved 64 ń 3 and 62 ń 3 p.c. of maximal heart rate during the exercise test on doxazosin and placebo, respectively. The baseline plasma concentration of potassium was similar both on active treatment and on placebo (5.1 ń 0.2 mmol/l on doxazosin and 4.9 ń 0.1 mmol/l on placebo). The serum potassium concentration increased significantly and to a similar extent during the tests. The mean rates of potassium increment during exercise were 8.4 ń 1.5 ćmol/l/min on doxazosin and 6.9 ń 1.3 ćmol/l/min on placebo. During the recovery period, the serum potassium concentration significantly decreased in both arms of the study. There were no significant changes in plasma sodium, calcium, and phosphate levels during the tests. Hydrogen ion concentration in blood, serum insulin and glucose, and plasma aldosterone and renin activity were similar before the exercise tests...

108/2523

Tytuł oryginału: Characterization of histamine H2-like receptors in duck cerebral cortical membranes by [3H]tiotidine binding.
Autorzy: Zawilska Jolanta, Zalewska-Kaszubska Jadwiga, Przybysz Marzena, Nowak Jerzy Z.
Źródło: Neurosci. Lett. 2002: 319 (3) s.149-152, il., bibliogr. 18 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ptaki

in vitro

Streszczenie angielskie: A selective (according to mammalian criteria) histamine (HA) H2-receptor radioligand ([3H]TIOT) was used to characterize HA receptors in duck cerebral cortex by an in vitro binding technique. The specific binding of [3H]TIOT to duck cerebral cortical membranes was found to be rapid, stable, saturable, reversible, and of high affinity. Saturation analysis resulted in a linear Scatchard plot suggesting binding to a single class of receptor binding sites with high affinity (Kd = 19.5 nM) and high capacity (Bmax - 356 fmol/mg protein). Competition studies showed the following relative rank order of potency of various HA receptor ligands to inhibit the [3H]TIOT binding: antagonists, tiotidine rantidine izolantidine ň cimetidine mepyramine thioperamide; agonists, HA ň 4-methylHA 2-methylHA dimaprit Rŕ-methylIHA. The biphasic nature of the displacement curve for HA and the effect of 5'-guanylimidodiphosphate indicate with [3H]TIOT represent either avian-specific H2-like HA receptors or a novel subtype of HA receptors, coupled to a signalling athway other than the adenylyl cyclase/cyclic adenosine monophosphate one.

109/2523

Tytuł oryginału: New stereocontrolled synthesis and biological evaluation of 5-(1'-Hydroxyalkyl)-3-methylidenetetrahydro-2-furanones as potential cytotoxic agents.
Autorzy: Janecki Tomasz, Błaszczyk Edyta, Studzian Kazimierz, Różalski Marek, Krajewska Urszula, Janecka Anna
Źródło: J. Med. Chem. 2002: 45 (5) s.1142-1145, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 312,133

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

in vitro

Streszczenie angielskie: A series of 3-methylidenetetrahydro-2-furanones 7 bearing various hydroxyalkyl substituents in position 5 were synthesized using novel diastereo- and enantioselective methodology. In vitro cytotoxicity data demonstrated that all prepared compounds were active against L-1210 and HL-60 tissue culture cells with 7e being the most potent (IC50 = 6.9 ćM). Also an increase in activity with an increase in lipophilicity of the substituents in the order H , alkyl phenyl was observed.

110/2523

Tytuł oryginału: Expression of mutant thyroid hormone nuclear receptors is associated with human renal clear cell carcinoma.
Autorzy: Kamiya Yuji, Puzianowska-Kuźnicka Monika, McPhie Peter, Nauman Janusz, Cheng Sheue-yann, Nauman Alicja
Źródło: Carcinogenesis 2002: 23 (1) s.25-33, il., tab., bibliogr. 59 poz.
Sygnatura GBL: 312,779

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Thyroid hormone (T3) regulates proliferation and differentiation of cells, via its nuclear receptors (TRs). These processes have been shown to be abnormally regulated during carcinogenesis. We have previously found aberrant expression of TRŕ and TRá mRNAs in renal clear cell carcinoma (RCCC), suggesting possible involvement of TRs in the carcinogenesis of RCCC. To understand the molecular actions of TRs in RCCC, cDNAs for TRá1 and TRŕ1 were cloned from 22 RCCC tissues and 20 surrounding normal tissues. Mutations were found in seven TRá1 and three TRŕ1 cDNAs. Two TRá1 cDNAs had a single mutation, while five TRá1 and three TRŕ1 had two or three mutations. Most of the mutations were localized in the hormone-binding domain. Using the TRs prepared by in vitro transcription/translation, we found that these mutations led to a loss of T3 binding activity and/or impairment in binding to thyroid hormone response elements (TREs). Furthermore, nuclear extracts from RCCC tissues also exhibited impairment in binding to TREs. These results indicate that the normal functions of TRs in RCCC tissues were impaired. Together with the aberrant expression patterns, these mutated TRs could contribute to the carcinogenesis of RCCC.

111/2523

Tytuł oryginału: Ludwik Hirszfeld as I remember him [1884-1954].
Autorzy: Skurska Zofia
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.5-8
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Hirszfeld

Ludwik 1844-1954

112/2523

Tytuł oryginału: Remembering Professor Ludwik Hirszfeld [1884-1954].
Autorzy: Benendo-Kapuścińska Bogusława
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.9-11, il.
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Hirszfeld

Ludwik 1884-1954

113/2523

Tytuł oryginału: Effective tumor immunotherapy: start the engine, release the brakes, step on the gas pedal, ... and get ready to face autoimmunity.
Autorzy: Tirapu Inigo, Mazzolini Guillermo, Rodriguez-Calvillo Mercedes, Arina Ainhoa, Palencia Belen, Gabari Izaskun, Malero Ignacio
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.13-18, bibliogr. 62 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Cellular immune responses can destory cancer cells, achieving the cure of experimental malignancies. An exapanding wealth of knowledge on the molecular basis of how to prime and amplify a T cell response has fueled a number of strategies successful at treating estabilished tumors (rather than merely preventing tumor grafting). The most efficacious approaches operate at different stages, including: 1) priming the immune response using tumor antigen-expressing dendritic cells or tumor cells transfected with genes that render them immunogenic, 2) sustaining and amplifying immunity using agonistic monoclonal antibodies against costimulatory molecules or immune-potentiating cytokines, and 3) eliminating mechanisms that self-regulate the strenght of the immune response, such as inhibitory receptors or regulatory T cells. A rational combination of such approaches holds great hope for cumulative and synergistic effects, but there is also evidence that they can open the flood-gates for unwanted inflammatory reactions. The next decade can be envisioned as the time when the first reproducibly efficacious combination regimes for cancer immunotherapy will become available and videly used in the clinic, as clinicians learn the best strategies and try to harness their potentially damaging effects.

114/2523

Tytuł oryginału: Caspases - their role in apoptosis and other physiological processes as revealed by knock-out studies.
Autorzy: Sadowski-Debbing Kenneth, Coy Johannes F., Mier Walter, Hug Hubert, Los Marek
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.19-34, il., tab., bibliogr. 113 poz.
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Caspases are crucial mediators of apoptosis, a form of physiological cell death. Their activation is carefully controlled by a philogenetically conserved death program, which is indispensable for the homeostasis and development of higher organisms. Dysregulation of apoptosis contributes to the pathogenesis of many human diseases. As effectors of the apoptotic machinery, caspases are considered potential therapeutic targets. In vitro studies have demonstrated the requirement of caspase activity for both the triggering phase as well as the execution of apoptosis, thus providing a molecular base for the fine-tuning of this process by pharmacological agents. The precise roles of the individual caspases in vivo and their functional relation to each other have been best demonstrated in genitically modified animals. The generation of single caspase-deficient mice have confirmed most of the data obtained in vitro and exposed some new aspects previously undetected in teh cell culture system. Interestingly, inactivation of many caspases revealed not only their expected participation in apoptotic as well as in the maturation of cytokines, but also provided hints about the role of at least some caspases in cell differentiation and stimulatory responses. In this review we will discuss what these studies have unveiled about the role of individual caspases in development, apoptosis, and inflammation, with particular focus on their role beyond the apoptotic process.

115/2523

Tytuł oryginału: The role of the innate and adaptive immune responses in Acanthamoeba keratitis.
Autorzy: Niederkorn Jerry Y.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.53-59, bibliogr. 49 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

okulistyka

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Infections of the corneal surface are an important cause of blindness. Protozoal, viral, bacterial, and helminthic infections of the cornea account for up to 9 milion cases of corneal blindness. Free-living amoebae of the genus Acanthamoeba produce a progressive infection of the cornea called Acanthamoeba keratitis. Disease is usually transmitted by Acanthamoeba trophozoites bound to soft contact lenses. Infection of the cornea is initiated when the parasite binds to the corneal epithelial surface. Recrudescence can occur and suggests that the adaptive immune response is not aroused by corneal Acanthamoeba infections. Systemic immunization with Acanthamoeba antigens elicits robust Th1 cell-mediated immunity and serum IgG antibody, yet fails to prevent the development of Acanthamoeba keratitis. However, immunization via mucosal surfaces induces anti-Acanthamoeba IgA antibodies in the tears and provides solid protection against the development of Acanthamoeba keratitis. Unlike other immune effector mechanisms that rely on cytolysis, inflammation, release of toxic molecules, or the induction of host cell death, the adaptive immune apparatus prevents Acanthamoeba infections of the cornea by simply preventing the attachment of the parasite to the epithelial surface. The beauty of this mechanisms lies in its exquisite simplicity and efficacy.

116/2523

Tytuł oryginału: Thermal glycation of proteins by D-glucose and D-fructose.
Autorzy: Kańska Urszula, Boratyński Janusz
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.61-66, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The dry thermal glycation method was used to conjugate D-glucose and D-fructose with bovine serum albumin. Reactions were conducted at 50-104řC for 30 min. Depending on temperature, different levels of substitution were achieved. In the case of D-glucose, average substitution levels of up to 53 mol glucose/mol bovine serum albumin were obtained. D-fructose turned out to be less reactive than D-glucose. The pH of the reaction mixture was also found to affect the efficacy of the glycation reaction. The levels of substitution were estimated using matrix-assisted laser desorption/ionisation time -of-flight mass spectrometry and SDS-polyacrylamide gel electrophoresis. Glycated molecules, and a process of glycation itself are implicated in diabetes complications. Hypothetically bovine spongiform encephelopathy (BSE) was also derived from glycation.

117/2523

Tytuł oryginału: Glycoforms of six serum glycoproteins in a patient with congenital disorder of glycosylation type I.
Autorzy: Ferens-Sieczkowska Mirosława, Zwierz Krzysztof, Midro Alina, Kątnik-Prastowska Iwona
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.67-73, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In this paper the occurrence and relative content of defectively glycosylated serum glycoforms in transferrin (Tf), ŕ1-acid glycoprotein (AGP), haptoglobin (Hp), ŕ1-antitripsin (ŕ1-AT), ŕ2-macroglobulin (ŕ2-MG) and ceruloplasmin (Cpl) in the serum of a patient with congenital disorder of glycosylation type I are reported. Blood samples were taken when the patient was 14 years old and then after a one-year interval. The patterns of glycoforms in both samples were compared. In 4 out of 6 examined glycoproteins, glycoforms lacking one and two oligosaccharide chains occurred. "Underglycosylated" glycoforms of ŕ2-MG and Cpl were not clearly detectable. Tf was shown to be affected with this defect to a higher extent than other glycoproteins, containing only 30 p.c. properly glycosylated molecules and also as much as 30 p.c. of the molecules lacking two glycan units. In Hp and ŕ1-AT the proportions of properly and defectively glycosylated forms were similar. This properly glycosylated form comprised 47 p.c. of the Hp and 51-55 p.c. of the ŕ1-AT molecules. As in AGP and Tf, about 30 p.c. the of molecules lacked one glycan unit. Twenty-one percent of the Hp molecules were devoid of two glycans, and this amount slightly increased in the course of the year. In ŕ1-AT, 19 and 17 p.c. of the molecules lacked two glycans in both samples, respectively. Only in AGP we did find a substantial difference between the two blood samples. In the course of the year, the amount of the form lacking 2-chains decreases from 12 to 3 p.c., resulting in a simultaneous icrease in the forms lacking...

118/2523

Tytuł oryginału: Comparison of leukocyte populations from bronchoalveolar lavage and induced sputum in the evaluation of cellular composition and nitric oxide production in patients with bronchial asthma.
Autorzy: Bieńkowska-Haba Małgorzata, Cembrzyńska-Nowak Monika, Liebhart Jerzy, Dobek Rafał, Liebhart Ewa, Siemieniec Iwona, Panaszek Bernard, Obojski Andrzej, Małolepszy Józef
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (1) s.75-82, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Bronchoalveolar lavage (BAL) or induced sputum (IS) techniques may provide lukocytes for the evaluation of airway inflammatory response in bronchial asthma. The aim of the present study was to compare features of leukocyte populations obtained by the two diffrent methods regarding the cell types and their activity in patients with bronchial asthma. The nitric oxide (NO) level released from the cells was measured as a marker of their activity. Pulmonary leukocytes were obtained from the BAL and IS of 11 asthmatic patients in stable condition at the time of the study. The BAL and IS leukocyte populations varied in cell count and NO production. Macrophages were the predominant leukocyte population in BAL (median (ME) = 83.0 p.c., range 67.9 - 88.4 p.c.), whereas sputum sediments were found to consist mainly of neutrophils (Me = 55.7 p.c., range 29.0 - 64.9 p.c.). The IS leukocytes released much more NO (p = 0.0022) than the BAL leukocytes released much more NO (p=0.0022) than the BAL leukocytes. In spite of these quantitative differences, a similar pattern of NO production was observed in BAL and in IS cells. Both BAL and IS leukocyte populations producted almost the same amounts of NO before and after lipopolysaccharide stimulation (p=0.9063, p= 0.4801, respectively). Furthermore, a slight positive correlation Spearman's rank (Rs)=0.5578, p=0.0594) was noticed between the neutrophil percentages and NO levels produced by BAL cells, whereas in IS a statistically significant correlation between the percentage of neutrophils and the levels of NO (Rs=0.6643, p=0.0184) was observed...

119/2523

Tytuł oryginału: The adipose tissue - a novel endocrine organ of interest to the nephrologist.
Autorzy: Więcek Andrzej, Kokot Franciszek, Chudek Jerzy, Adamczak Marcin
Źródło: Nephrol. Dial. Transplant. 2002: 17 (2) s.191-195, il., bibliogr. 39 poz.
Sygnatura GBL: 312,872

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

120/2523

Tytuł oryginału: Efficacy of the new long-acting formulation of lanreotide (Lanreotide Autogel) in the management of acromegaly.
Autorzy: Caron Ph., Beckers A., Cullen D. R., Goth M. I., Gutt B., Laurberg P., Pico A. M., Valimaki M., Zgliczyński W.
Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (1) s.99-104, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 310,620

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Lanreotide Autogel is a new long-acting aqueous preparation of lanreotide for the treatment of acromegaly and is administered by deep sc injection from a small volume, prefilled syringe. The aim of this study was to evaluate the efficacy and safety of this new ong-acting formulation in a large population of acromegalic patients previously responsive to lanreotide 30 mg, im (substained release microparticle formulation). Lanreotide Autogel was administered by deep sc injection every 28 d to 107 patients (54 males and 53 females; mean age, 54 ń 1.2 yr). All patients had been treated with lanreotide (30 mg) for at least 3 months before study entry and had a mean GH level less than 10 ng/ml after at least 4 subsequent im injections every 14 d (48 p.c.), 10 d (32 p.c.), or 7 d (20 p.c.). Treatment was switched from lanreotide 30 mg injected every 14, 10, or 7 d to 60, 90, or 120 mg lanreotide Autogel, respectively, every 28 d. After three fixed dose injections of lanreotide Autogel, mean lanreotide levels were similar to those obtained at steady state with lanreotide 30 mg. During lanreotide Autogel treatment, the control of acromegalic symptoms was comparable with that previoulsy achived during lanreotide 30 mg treatment. After 3 injections of lanreotide Autogel, mean GH (2.87 ń 0.22 ng/ml) and IGF-I (317 ń 15 ng/ml) values were comparable with those recorded at the end of lanreotide 30 mg treatment (GH, 2.82 ń 0.19 ng/ml; IGF-I, 323 ń 16 ng/ml). GH levels below 2.5 ng/ml and age-/sex-normalized IGF-I were achived in 33 p.c. and 39 p.c. of patients during lanreotide 30 mg and lanreotide Autogel treatment, respectively...

121/2523

Tytuł oryginału: Surgical view of the treatment of patients with hepatoblastoma. Results from the First Prospective Trial of the International Society of Pediatric Oncology Liver Tumor Study Group (SIOPEL-1).
Autorzy: Schnater J. Marco, Aronson Danil C., Plaschkes Jack, Perilongo Giorgio, Brown Julia, Otte Jean-Bernard, Brugieres Laurences, Czauderna Piotr, MacKinlay Gordon, Vos Anton
Źródło: Cancer 2002: 94 (4) s.1111-1120, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 311,015

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background. Surgical resection is the cornerstone of treatment for patients with hepatoblastoma (HB). The Society of Pediatric Oncology Liver Tumor Study Group launched its first prospective trial (SIOPEL-1) with the intention to treat all patients with preoperative chemotherapy and delayed surgical resection. The objective of this article was to assess the assumed surgical advantages of primary chemotherapy. Methods. Between 1990 and 1994, 154 patients age 16 years with HB were registered on SIOPEL-1. The pretreatment extent of disease was assessed, and, after undergoing biopsy, patients were treated with cisplatin 80 mg/mý intravenously over 24 hours and doxorubicin 60 mg/mý intravenously over 48 hours by continuous infusion (PLADO). Generally, tumors were resected after four of a total of six courses of PLADO. Results. One hundred twenty eight patients underwent surgical resection (13 patients underwent primary surgery, and 115 patients underwent delayed surgery after PLADO). A pretreatment surgical biopsy was performed in 96 of 128 patients (75 p.c.). Biopsy complications occurred in 7 of 96 patients (7 p.c.). Twenty-two patients showed pulmonary metastases at the time of diagnosis, and 7 patients underwent thoracotomy. Operative morbidity and mortality were 18 p.c. and 5 p.c., respectively. Complete macroscopic surgical resection was achieved in 106 patients (92 p.c.), including 6 patients who underwent orthotopic liver transplantation. The actuarial 5-year event free survival (EFS) rate for all 154 patients in the study was 66 p.c., and the overall survival (OS) rate was (75 p.c.)...

122/2523

Tytuł oryginału: Elevated soluble intercellular adhesion molecule-1 levels in obesity: relationship to insulin resistance and tumor necrosis factor-ŕ system activity.
Autorzy: Strączkowski Marek, Lewczuk Piotr, Dzienis-Strączkowska Stella, Kowalska Irina, Stępień Agnieszka, Kinalska Ida
Źródło: Metabolism 2002: 51 (1) s.75-78, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 303,583

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Intercellular adhesion molecule-1 (ICAM-1) is 1 of the possible factors linking obesity and diabetes with cardiovascular disease, however, the mechanism of the increase in ICAM-1 concentration in obesity remains unclear. Therefore, the aim of the present study was to assess plasma soluble ICAM-1 (sICAM-1) levels on obese subjects with normal glucose tolerance and to evaluate whether those levels may be related to insulin resistance and tumor necrosis factor-ŕ (TNFŕ) system activity. The study was performed in 8 lean and 15 obese subjects. Anthropometric and biochemical parameters were measured, and insulin sensitivity was evaluated using the euglycemic hyperinsulinemic clamp technique (insulin infusion, 50 mU x kg**-1 x h**-1). Obese subjects were markedly more hyperinsulinemic and insulin resistant and had higher plasma soluble TNF receptor 2 (sTNFR2) and sICAM-1 levels. sICAM-1 was related positively to body mass index (BMI), waist-to-hip ratio (WHR), percent of body fat, glycated hemoglobin (HbA1c), plasma insulin and triglycerides (TG), TNFŕ, and sTNFR2 and negatively to insulin sensitivity. Multiple regression analysis showed that only sTNFR2 and insulin sensitivity were independent predictors of sICAM-1 concentrations and were responsible for 66 p.c. of sICAM-1 variability. We conclude that an increase in plasma sICAM-1 concentration in obesity is related to TNFŕ system activation and insulin resistance.

123/2523

Tytuł oryginału: Enhanced T cells interactions with extracellular matrix proteins in infertile women with endometriosis.
Autorzy: Jerzak Małgorzata, Baranowski Włodzimierz, Rechberger Tomasz, Górski Andrzej
Źródło: Immunol. Lett. 2002: 81 (1) s.65-70, il., tab., bibliogr. 53 poz.
Sygnatura GBL: 312,663

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Essential features of endometriosis involve interactions with extracellular matrix (ECM). Recent data emphasize the important role of ECM proteins in the regulation of T cell function. The aim of this study was to determine activated T cell adhesion to ECM proteins in infertile women with endometriosis. Nine women with endometriosis diagnosed by laparoscopy according to the Revised American Fertility Society classification and ten normal healthy women with a previous successful pregnancy outcome were studied. We investigated phorbol acetate myristate (PMA) or phytohemaglutinin (PHA) activated peripheral blood T cell adhesion to the following proteins of ECM: collagen IV (C-IV), elastin (E) and fibronectin (Fn). In addition, CD4, CD8, CD29, CD45RO exspression on peripheral CD3+ T cells were studied using flow cytometry. We determine that PHA-activated T cell adhesion to C-IV and Fn are significantly higher in infertile women with endometriosis when compared to normal healthy women (P 0.05). No significant differences were noted in T cell's surface antigens expression between study groups. Our data suggest the existence of disturbed T cell-ECM interactions in infertile women with endometriosis. Further studies are needed to determine the role of these abnormalities in the pathogenesis of endometriosis.

124/2523

Tytuł oryginału: Allelic distribution of complement components BF, C4A, C4B, and C3 in Psoriasis vulgaris.
Autorzy: Cisło Maria, Hałasa Jolanta, Wąsik Feliks, Nockowski Piotr, Prussak Magdalena, Mańczak Maria, Kuśnierczyk Piotr
Źródło: Immunol. Lett. 2002: 80 (3) s.145-149, tab., bibliogr. 58 poz.
Sygnatura GBL: 312,663

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Psoriasis vulgaris is a multifactorial disease; the strongest association was established with the HLA complex. The actual disease-predisposing gene(s) has not been identified yet, but several genes from this region were examined in addition to HLA-C and -B. However, HLA-linked complement component polymorphic genes were not extensively studied. Therefore, we typed 67 psoriatic patients for alleles of the HLA-linked complement components BF, C4A and C4B. Alleles of C3, encoded on another chromosome, were established in parallel as a negative control. Frequencies in patients were compared with thise in unrelated healthy controls, 100 individuals for C4A and C4B, 890 for BF and 4719 for C3. We found no association of BF alleles with disease, similarly to C3. Among C4 alleles, C4B*3 was present in 13,4 p.c. of patients as compared with 1 p.c. of controls (OR, 15.36; 95 p.c. CI, 1.897 - 124.42; P = 0.0009), and C4A*6 was present in 19.4 p.c. of patients versus 7 p.c. of controls (OR, 3.20; 95 p.c. CI, 1.202 - 8.508; P = 0.0155). The high frequency of C4B*3 in psoriatics has not been described so far. These results suggest a contribution of C4 genes themselves or a closely linked gene to the susceptibility to psoriasis.

125/2523

Tytuł oryginału: Endothelin in the mechanism of endothelial injury and neutrophil adhesion in the post-ischemic guinea-pig heart.
Autorzy: Kurzelewski Michał, Czarnowska Elżbieta, Beręsewicz Andrzej
Źródło: Eur. J. Pharmacol. 2002: 434 (1/2) s.95-107, il., tab., bibliogr. s. 105-107
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnki morskie

Streszczenie angielskie: This study addressed the hypothesis that endothelin promotes neutrophil accumulation in ischemic/reperfused myocardium, not only via its direct effect on neutrophils, but also because it mediates post-ischemic endothelial injury. Langendorff-perfussed guinea-pig hearts were subjected to 30 min ischemia/35 min reperfusion, and infusion of neutrophils between 15 and 25 min of reperfusion. The infusion of the endothelin ETA/ETB receptor antagonist, tezosentan, the endothelin ETA receptor antagonist, BQ 123 [cyclo(-D-Trp-D-Asp-Pro-D-Val-Leu-], and superoxide dismutase was terminated at reperfusions, 5 min before the start of the neutrophil infusion, to avoid the contact of the drugs with neutrophils. Coronary flow responses to acetylcholine and nitroprusside were used as measures of endothelium-dependent and -independent vascular function, respectively. Neutrophil adhesion and endothelium glycocalyx ultrastructure were assessed in histological preparations. Ischemia/reperfusion resulted in a 54 p.c.-impaired acetylcholine response, endothelium glycocalyx disruption, and enhanced neutrophil adhesion (21.6 p.c. of microvessels contained neutrophils vs. 2.6 p.c. in sham group), the latter prevented by a selectin blocker, sulfatide, 20 ćg/ml. These alterations were completely prevented by 0.5 and 5 nM, but not 0.05 nM, tezosentan, and were greatly attenuated by BQ 123, 1 and 10 nM. The glycocalyx-protective effect of these interventions preceded their effect on neutrophil adhesion. Superoxide dismutase, 150 IU/ml, reported before by us to protect post-ischemic endothelium glycocalyx, here prevented the post-ischemic endothelial dysfunction and neutrophil adhesion...

126/2523

Tytuł oryginału: Successful use of activated recombinant factor VII to control bleeding abnormalities in a patient with a left ventricular assist device.
Autorzy: Ziętkiewicz Mirosław, Garlicki Mirosław, Domagała Jerzy, Wierzbicki Karol, Drwiła Rafał, Pfitzner Roman, Dziatkowiak Antoni
Źródło: J. Thorac. Cardiovasc. Surg. 2002: 123 (2) s.384-385, bibliogr. 5 poz.
Sygnatura GBL: 310,230

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

127/2523

Tytuł oryginału: N-acetyltransferase 2 polymorphism in sporadic Parkinson's disease in a Polish population.
Autorzy: Białecka Monika, Gawrońska-Szklarz Barbara, Droździk Marek, Honczarenko Krystyna, Stankiewicz Jan
Źródło: Eur. J. Clin. Pharmacol. 2002: 57 (12) s.857-862, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 312,148

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Objective: A genetic background of Parkinson's disease has been suggested, including genes implicated in xenobiotic metabolism. So far, many candidate genes responsible for the occurrence of the disease have been enumerated. This study was carried out to determine the presence of N-acetyltransferase 2 polymorphism in Parkinson's disease patients in a Polish population. Methods: Fifty-four patients with diagnosed sporadic Parkinson's disease and 81 healthy individuals were enrolled into the study. The N-acetyltransferase 2 alleles (*4, *5, *6 and *7) were identified using polymerase chain reaction-restriction fragment length polymorphism methods with DNA extracted from peripheral blood. Results: A preponderance of slow acetylators in patients with Parkinson's disease was demonstrated. Among 54 subjects with parkinsonism, 64.8 p.c. were homozygous for two mutated alleles responsible for the slow-acetylator phenotype. In the control group, a predominance of fast acetylators was noted. Subjects homozygous and heterozygous with genotypes determining fast acetylation constituting 53 p.c. of subjects, whereas 47 p.c. were slow acetylators. Comparison of the two groups of the study, i.e. Parkinson's disease and healthy individuals, revealed a statistically significant predominance of slow acetylators in Parkinson's disease patients (P 0.05). The risk of Parkinson's disease development was more than two times greater in slow acetylators than healthy subjects. The frequency of point mutations was similar both in patients with Parkinson's disease and the healthy cotrols. Conclusion: Slow-acetylation genotype may be an important factor of individual susceptibility to Parkinson's disease.

128/2523

Tytuł oryginału: Serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in different histological variants of rheumatoid synovitis.
Autorzy: Klimiuk P. A., Sierakowski S[tanisław], Latosiewicz R[obert], Cylwik B[ohdan], Skowroński J[an], Chwiecko J.
Źródło: Rheumatology 2002: 41 (1) s.78-87, il., tab., bibliogr. 50 poz.
Sygnatura GBL: 303,271

Hasła klasyfikacyjne GBL:

reumatologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective. Rheumatoid synovitis is characterized by an invasive and tissue-destructive infiltrate of lymphocytes, macrophages and synoviocytes. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) produced by these cells are important in the remodelling of the articular tissue in rheumatoid arthritis (RA). The aim of this study was to explore whether the serum concentrations of MMPs and their inhibitors were correlated with the histological appearance of the disease. Methods. Tissue and serum samples were obtained from 37 patients with clinically active RA and 30 with osteoarthritis (OA). Morphological analysis allowed the division of RA synovial specimens into two distinct types. In 22 samples only diffuse infiltrates of mononuclear cells without further microanatomical organization were found. In 15 specimens we observed lymphocytic conglomerates with germinal centre-like structures. Serum concentrations of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), gelatinase B (MMP-9), TIMP-1 and TIMP-2 were measured with an ELISA technique. Results. Unique serum profiles of MMPs and TIMPs were identified in each of the two histological types of RA synovitis. The serum concentrations of MMP-1, MMP-3 and MMP-9 were higher in RA patients than in OA patients used as a control group (P 0.001 for all comparisons). These three MMPs dominated in the serum of RA patients with follicular synovitis compared with those diffuse synovitis (P 0.05, P 0.01 and P 0.001 respectively)...

129/2523

Tytuł oryginału: GLP-1 as a satiety fctor in children with eating disorders.
Autorzy: Tomasik P. J., Sztefko K., Malek A.
Źródło: Horm. Metab. Res. 2002: 34 (2) s.77-80, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 312,226

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: GLP-1, with its insulinotropic properties and direct action on satiety center in the brain, may be the main hormone regulating the amount of ingested food. In this study, GLP-1 secretion was investigated in age-matched adolescent girls (14 ń 2 years): 13 with anorexia nervosa (BMI 14.8 ń 1.4 kg/mý), 13 with simple obesity (BMI 33.0 ń 3.3 kg/mý) and 10 healthy girls as a control group (BMI 21.6 ń 0.7 kg/mý). Each girl was subjected to OGTT and standard meal tests after a 12h overnight fast. Blood samples were collected before and 15, 30, 60, and 120 min after the stimulation. The mean fasted GLP-1 levels in simple obesity group (1.6 ń 0.3 pmol/l) and in anorexia nervosa group (1.7 ń 0.3 pmol/l) were significantly lower than those in the control group (2.6 ń 0.4 pmol) (p 0.05) in both cases). The highest peak concentration of GLP-1 was observed in the control group after both stimuli. In each group, the mean integrated GLP-1 outputs were almost twice as high after OGTT than after the test meal (p 0.001 in each case). In our opinion, low secretion of GLP-1 in girls with simple obesity may seriously and negatively influence the course of this disease. On the other hand, low GLP-1 levels in girls with anorexia nervosa are beneficial and promote appetite.

130/2523

Tytuł oryginału: Increased nitric oxide bioavailability in endothelial cells contributes to the pleiotropic effect of cerivastatin.
Autorzy: Kalinowski Leszek, Dobrucki Lawrence W., Brovkovych Viktor, Maliński Tadeusz
Źródło: Circulation 2002: 105 (8) s.933-938, il., bibliogr. 17 poz.
Sygnatura GBL: 311,033

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Although statins preserve endothelial function by reducing serum cholesterol levels, it has been suggested they may also stimulate nitric oxide (NO) synthase in endothelium with concurrent increase in superoxide (O2-) generation, leading to impairment of NO activity. Therefore, measurements of biologically active NO and O2 - in endothelium after exposure to the HMG-CoA reductase inhibitor cerivastatin were undertaken to evaluate its potential effect on NO biological activity. Methods and Results - Highly sensitive electrochemical NO and O2 - microsensors were placed near the surface of a single human umbilical vein endothelial cell, and the kinetics of NO and O2 - release were recorded in vitro. Cerivastatin demonstrated a time-dependent effect on NO release in endothelial cels. The initial release (approximately the first 3 minutes) was concentration-dependent (0.01 to 10 ćmol/L) and was similar to that observed for typical NO synthase agonists calcium ionophore or acetylcholine. Cerivastatin stimulated NO release at a favorable rate and scavenged O2-, which led to the preservation of the active concentration of NO. The sustained effect (after ÷ 6 hours) of cerivastatin on endothelium was associated with an ÷ 35 p.c. increase in NO release as compared with the initial effect. In contrast to the initial effect, the sustained effect of cerivastatin was shown at concenrations ÷ 100-fold lower and was dependent on inhibition of endothelial HMG-CoA reductase. Conclusions - These data provide direct evidence to prove that in the presence of cerivastatin, the NOS system in endothelium operates with high efficiency toward increasing NO activity by activation of NO release and by concurrent inactivation of O2-.

131/2523

Tytuł oryginału: Juvenile idiopathic arthritis (JIA) is primarily associated with HLA-DR8 but not DQ4 on the DR8-DQ4 haplotype.
Autorzy: Smerdel A., Ploski R., Flato B., Musiej-Nowakowska E., Thorsby E., Forre O.
Źródło: Ann. Rheum. Dis. 2002: 61 (4) s.354-357, tab., bibliogr. 17 poz.
Sygnatura GBL: 310,264

Hasła klasyfikacyjne GBL:

reumatologia

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Juvenile idiopathic arthritis (JIA) is strongly associated with the DR8-DQ4 haplotype. The genes encoding DR8 and DQ4 are in strong linkage disequilibrium (LD) and oocur together on the same HLA haplotype in almost all patients and controls. Because of the strong LD it is not clear whether DR8, DQ4, or both, are primarily associated with JIA. Objective: To unveil the primary association of JIA - that is, with DR8 or DQ4. Methods: DRB1, DQA1, and DQB1 alleles of 585 Norwegian and 47 Polish unrelated patients with JIA (categorised as pauciarticular and rheumatoid factor negative polyarticular JIA), and of 3155 Norwegian and 158 Polish unrelated controls, were typed using a polymerase chain reaction or oligonucleotide hybridisation and sequence-specific primers method. Results: Several haplotypes which encoded DR8 (that is, carried DRB1*08) and which did not encode DQ4 (that is, did not carry DQA1*0401) were found. Such haplotypes were found in three Norwegian patients and two controls (p = 0.029). In the Polish population such haplotypes were found among four patients with JIA and two controls (p = 0.025). No haplotypes which carried DQA1*0401 and DQB1*0402 in the absence of DRB1*08 were found, either among patients with JIA (Polish and Norwegian) or among the controls (Polish). Conclusion: On the DR8-DQ4 haplotype the DRB1*08 allele is primarily associated with JIA.

132/2523

Tytuł oryginału: Estrogen inhibits systemic T cell expression of TNF-ŕ and recruitment of TNF-ŕ+ T cells and macrophages into the CNS of mice developing experimental encephalomyelitis.
Autorzy: Ito Atsushi, Buenafe Abigail C., Matejuk Agata, Zamora Alex, Silverman Marc, Dwyer Jami, Vandenbark Arthur A., Offner Halina
Źródło: Clin. Immunol. 2002: 102 (3) s.275-282, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 305,911

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Estrogen treatment has been found to have suppressive activity in several models of autoimmunity. To investigate the mechanism of 17á-estradiol (E2) suppression of experimental autoimmune encephalomyelitis, we evaluated E2 effects on TNF-ŕ experssion in the central nervous system (CNS) and spleen of C57BL/6 mice immunized with MOG 35-55/CFA. Kinetic analysis demonstrated that E2 treatment drastically decreased the recruitment of total inflammatory cells as well as TNF-ŕ+ macrophages and T cell into the CNS at disease onset. In contrast, E2 had only moderate effects on the erlatively high constitutive TNF-ŕ expression by resident CNS microglial cells. E2 treatment also had profound inhibitory effects on expression of TNF-ŕ by splenic CD4+ T cells, including those responsive to MOG 35-55 peptide. We propose that the mechanism of E2 protection may involve both systemic inhibition of TNF-ŕ expression and local (CNS) ercruitment of inflammatory cels, with modest effects on TNF-ŕ expression by resident CNS microglial cells.

133/2523

Tytuł oryginału: Extent of, and factors associated with, delay to hospital presentation in patients with acute coronary disease (the GRACE registry).
Autorzy: Goldberg Robert J., Steg Philippe Gabriel, Sadiq Immad, Granger Christopher B., Jackson Elizabeth A., Budaj Andrzej, Brieger David, Avezum Alvaro, Goodman Shaun
Źródło: Am. J. Cardiol. 2002: 89 (7) s.791-796, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 304,619

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Our primary study aim was to examine extent of, and factors associated with, delay in seeking medical care in a large multinational registry of patients with acute myocardial infarction (AMI) and unstable angina pectoris. A secondary goal was to examine the relation between duration of prehospital delay and receipt and timing of coronary reperfusion strategies. Investigators from 14 countries are participating in the Global Registry of Acute Coronary Events (GRACE) project. The study sample consisted of 3,693 patients with ST-segment elevation AMI, 2,935 with non-ST-segment elevation AMI, and 3,954 patients with unstable angina hospitalized between 1999 and 2001. The average and median delay times were longest in patients with non-ST-segment elevation AMI (6.1 and 3.0 hours, respectively) followed by patients with unstable angina (5.6 and 3.0 hours) and those with ST-segment elevation AMI (4.7 and 2.3 hours). Approximately 41 p.c. of patients with ST-segment elevation AMI presented to the 94 study hospitals within 2 hours of the onset of acute coronary symptoms; the compared with approximately one third of patients with non-ST-segment elevation AMI and unstable angina. Several demographic and clinical characteristics were associated with prehospital delay. In patients with ST-segment elevation AMI, duration of prehospital delay was inversely related to the receipt of thrombolytic therapy, but was inconsistently related to the use pf percutaneous coronary interventions. The results of this study demonstrate that a large proportion of patients continue to exhibit prolonged delay in seeking medical care after the onset of acute coronary symptoms and remain in need of targeted educational efforts to reduce extent of delay.

134/2523

Tytuł oryginału: Fine-needle aspiration biopsy of the thyroid in an area of endemic goitre: influence of restored sufficient iodine supplementation on the clinical significance of cytological results.
Autorzy: Słowińska-Klencka Dorota, Klencki Mariusz, Sporny Stanisław, Lewiński Andrzej
Źródło: Eur. J. Endocrinol. 2002: 146 (1) s.19-26, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 301,344

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: The iodine status of the population of Poland has markedly improved over the past years. The aims of this paper were: (i) to examine the diagnostic, value of fine-needle aspiration biopsy (FNAB) of the thyroid in goitre endemic regions (in conditions of improved iodine supply), and (ii) to find whether the changes in iodine supply have already influenced the clinical interpretation of cytological results. Methods: Cytological diagnoses, based on 3782 aspirates, obtained from 3572 patients during the years 1985-1999, were verified by reference to the results of postoperative examinations. The relative occurrences of selected cytological results in 1992-1999 were also compared (patients not subjected to surgery were included). Results: We have found that the frequenty of neoplastic lesions significantly decreased throughout the examined period (P 0.02). The ratio of the follicular carcinoma frequency increased from 1.7 during 1992-1993 up to 8.0 during 1998-1999 (P 0.05). The frequency of cytologically diagnosed chronic thyroiditis increased from 1.5 p.c. in 1992 to 5.7 p.c. in 1999 (P 0.001); the percentage of cytological diagnosis of 'follicular neoplasm' decreased during the same time (P 0.001). The risk of malignancy significantly lowered in the cytological diagnoses of 'follicular neoplasm' from 15 p.c. during 1985-1993 to 6 p.c. during 1996-1999 (P 0.05). Conclusions: The diagnostic value of FNAM during the period without proper iodine prophylaxis did not differ significantly from that during the last examined period. However, the changes in iodine supply have markedly and promptly affected the clinical significance of particular cytological results.

135/2523

Tytuł oryginału: Angiogenesis of endocrine gland tumours - new molecular targets in diagnostics and therapy.
Autorzy: Stępień H. M., Kołomecki K., Pasieka Z., Komorowski J., Stępień T., Kuzdak K.
Źródło: Eur. J. Endocrinol. 2002: 146 (2) s.143-151, tab., bibliogr. 96 poz.
Sygnatura GBL: 301,344

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Angiogenesis in one of the key stages in the development of neoplastic tumours, in which a small group of mutated cells transforms into a large malignant tumour metastasising to the neighbouring tissues and organs. The studies on the significance of neoangiogenesis in the progression of endocrine gland neoplasms have recently become one of the most rapidly evolving branches of molecular endocrinology. The induction of angiogenesis has been demonstrated to result from the imbalance between positive and negative factors which control this process. Our paper presents the results of current studies on the role of factors such as molecular markers of angiogenesis (e.g. vascular endothelial growth factor and basic fibroblast growth factor), metalloproteinases (which regulate the decomposition of the extracellular matrix) and their inhibitors, and adhesive molecules (e.g. soluble vascular cellular adhesion molecule-1 and soluble intracelular adhesion lecule-1) in the pathogenesis and diagnostics of endocrine gland tumours in humans. Also, we discuss new therapeutic strategies for inhibiting the growth of neoplasms by blocking the formation of blood vessels using angiogenesis antagonists, which inhibit various stages of angiogenesis. More and more data are being accumulated suggesting that these preparations could, in the near future, be used in the pharmacotherapy of some endocrine gland neoplasms.

136/2523

Tytuł oryginału: O**6-Alkylguanine-DNA-alkyltransferase activity in peripheral leukocytes, smoking and risk of lung cancer.
Autorzy: Boffetta Paolo, Nyberg Fredrik, Mukeria Anush, Benhamou Simone, Constantinescu Vali, Batura-Gabryel Halina, Brske-Hohlfeld Irene, Schmid Giovanni, Simonato Lorenzo, Pelkonen Pivi, Hall Janet
Źródło: Cancer Lett. 2002: 180 (1) s.33-39, tab., bibliogr. 25 poz.
Sygnatura GBL: 306,023

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: The level of activity of O**6-alkalguanine-DNA-alkytransferase (AGT), a DNA repair enzyme, in blood lymphocytes may be a marker of susceptibility to lung cancer. We measured the AGT activity level, expressed as plomes of repaired bases/mg protein, in leukocytes of 153 lung cancer cases (of whom 80 were never smokers) and 106 controls (76 never smokers) enrolled in eight centres from seven countries. Subjects were interviewed with respect to active smoking and exposure to environmental tobacco smoke (ETS). Among never smokers, the odds ratios (ORs) of lung cancer were 1.3 (95 p.c. confidence interval 0.5-3.9), 1.5 (0.6-4.1) and 1.4 (0.5-3.8) in quartiles of decreasing AGT activity level, as compared to the upper quartile (P value of test for linear trend 0.6). Corresponding ORs among smokers were 3.4 (0.9-13), 2.0 (0.5-8.3) and 0.4 (0.1-1.6) (P value of test for linear trend 0.4). No interaction was suggested between AGT activity level and either cumulative smoking or exposure to ETS. Reduced AGT activity was not clearly associated with increased lung cancer risk in either smokers or non-smokers. However, the small size of our study argues for a prudent interpretation of our results.

137/2523

Tytuł oryginału: Contrast echocardiography and medical economics: looking into the crystal ball.
Autorzy: Distante A., Dankowski R., Mincarone P., Leo C. G., Gianicolo E., Voci P., Morales M. A., Rovai D.
Źródło: Eur. Heart J. 2002: 4 supl. C s.C39-C47, tab., bibliogr. 78 poz.
Sygnatura GBL: 306,213

Hasła klasyfikacyjne GBL:

ekonomika

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The past decade has witnessed the development of contrast echocardiography. This technique has demonstrated clinical utility in some applications and extremely promising results have been reported for others, although there are still several obstacles to its acceptance by regulatory authorities and routine users. In addition, the eficacy and cost-effectiveness must be proven against 'gold standard' techniques, which in several cases are just accepted standards. Furthermore, the competition is quite strong both between new techniques and with established ones, and all of the imaging modalities need to find their most appropriate role within this comples environment.

138/2523

Tytuł oryginału: Plasma fibrinogen and troponin I in acute coronary syndrome and stable angina.
Autorzy: Gil Maciej, Zarębiński Maciej, Adamus Jerzy
Źródło: Int. J. Cardiol. 2002: 83 (1) s.43-46, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We aimed to determine whether there is a stratification among patients with different stages of coronary artery disease with respect to plasma fibrinogen levels, and to assess diagnostic value of plasma fibrinogen in comparison to troponin I in patients with acute coronary syndrome. Fifty-one consecutive patients presenting to our department with acute coronary syndrome within the last 24 h and 52 patients with stable angina with no episode of unstable disease within the last month were analysed. Forty-nine patients with acute coronary syndrome in which both troponin I and fibrinogen levels were prsent were further evaluated. Blood was collected on admission for routine laboratory tests. Statistical analysis was done using Student's t-test, Pearson correlation and chi-square test, P 0.05 being considered statistically significant. Plasma fibrinogen levels (g/l) were significantly higher in patients presenting with unstable than with stable angina (3.87 ń 1.2 vs. 3.26 ń 0.65 P = 0.002). We have found significant correlation between fibrinogen and troponin I levels in coexist and correlate. These results seem to confirm the role of this acute phase protein in the pathophysiology of acute coronary syndrome.

139/2523

Tytuł oryginału: Anthrapyridones, a novel group of antitumour non-cross resistant anthraquinone analogues. Synthesis and molecular basis of the cytotoxic activity towards K562/DOX cells.
Autorzy: Tarasiuk J., Stefańska B., Plodzich I., Tkaczyk-Gobis K., Seksek O., Martelli S., Garnier-Suillerot A., Borowski E.
Źródło: Br. J. Pharmacol. 2002: 135 (6) s.1513-1523, il., tab., bibliogr. s. 1522-1523
Sygnatura GBL: 301,182

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: 1. Multidrug resistance (MDR) to antitumour agents, structurally dissimilar and having different intracellular targets, is the major problem in cancer therapy. MDR phenomenon is associated with the presence of membrane proteins which belong to the ATP-binding cassette family transporters responsible for the active drug efflux leading to the decreased intracellular accumulation. 2. The search of new compounds able to overcome MDR is of prime importance. 3. recently we have synthesized a new family of anthrapyridone compounds. The series contained derivatives modified with appropriate hydrophobic or hydrophylic substituents at the side chain. 4. The interaction of these derivatives with erythroleukina K562 sensitive and K562/DOX resistant (overexpressing P-glycoprotein) cell lines has been examined. The study was performed using a spectrofluorometric method which allows to continuously follow the uptake and efflux of fluorescent molecules by living cells. 5. It was demonstrated that the increase in the lipophilicity of anthrapyridones favoured the very fast cellular uptake exceeding the rate of P-gp dependent efflux out of the cell. For these derivatives, very high accumulation (the same for sensitive and resistant cells) was observed and the in vitro biological data confirmed that these compounds exhibited comparable cytotoxic activity towards sensitive and P-gp resistant cell line. In contrast, anthrapyridones modified with hydrophylic substituents exhibited relatively low kinetics of cellular uptake...

140/2523

Tytuł oryginału: Carvedilol does not modulate moderate exercise-induced hyperkalemia in hemodialysis patients.
Autorzy: Nowicki M., Szewczyk-Seifert G., Klimek D., Kokot F[ranciszek]
Źródło: Clin. Nephrol. 2002: 57 (5) s.352-358, il., tab., bibliogr. s. 357-358
Sygnatura GBL: 312,466

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background and aim: Non-selective á-adrenergic blockers may cause yperkalemia in patients with end-stage renal failure. In contrast, ŕ-adrenergic blockade has been found to decrease the hyperkalemic effect of physical exercise in healthy subjects, although we were unable to confirm this effect in hemodialysis patients. In a crossover design, we studied the effect of carvedilol, a non-selective á-adrenergic blocker with an additional ŕ1-blocking activity, on exercise-induced hyperkalemia in 17 anuric hemodialysis patients. Methods: All subjects were hiven either carvedilol (25 mg/day) or placebo for 2 weeks in a random order with a 2-week wash-out period. At the end of each treatment period they underwent a 30-minute exercise test on a bicycle ergometer with a fixed load of 20 W. Results: The treatment with carvedilol caused a significant decrease in blood pressure. Serum potassium before exercise tests was similar (5.37 ń 0.2 and 5.24 ń 0.2 mmol/l on carvedilol and placebo, respectively; mean ń SE). During the exercise, serum postassium oncreased significantly (p 0.001 in both tests) and subsequently decreased during 30 minutes of recovery (p 0.05). The mean rate of potassium increment during the exercise was similar (23.3 ń 3.3 ćmol/min on carvedilol and 20.0 ń 3.6 ćmol/l/min on placebo). during recovery, the mean rate of potassium decrement was 5.0 ń 3.0 ćmol/l/min and 6.7 ń 2.7 ćmol/l/min, respectively. Serum sodium, ionized calcium, insulin and plasma renin activity were similar before the exercise tests and did not change during the exercise. Conclusion: Carvedilol does not enhance the hyperkalemic effect of moderate physical exercise in anuric hemodialysis patients.

141/2523

Tytuł oryginału: Expression of intercellular adhesion molecule-1 on the surface of peripheral blood and decidual lymhocytes of women with pregnancy-induced hypertension.
Autorzy: Wilczyński Jacek R., Banasik Małgorzata, Tchórzewski Henryk, Głowacka Ewa, Malinowski Andrzej, Szpakowski Marian, Lewkowicz Przemysław, Wieczorek Artur, Zeman Krzysztof, Wilczyński Jan
Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 102 (1) s.15-20, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Objective: If overexpression of intercelular adhesion molecule-1 (CD54) on lymphocytes exists it could have important implications for the pathophysiology of pregnancy-induced hypertension (PIH). Study design: CD54 "in vitro" expression (described as (p.c.) of CD54 + cells and CD54 mean fluorescence intensity, MFI) on the peripheral blood and decidual lymphocytes of pregnant with preeclampsia (PE) (n = 16), transient hypertension (TH) (n = 12), and controls (n = 9). Results: The percent (p.c.) of CD54 + peripheral blood lymphocytes, CD54 MFI on them and CD54 MFI on decidual lymphocytes were increased especially in PE. During PE the (p.c.) of CD54 decidual lymphocytes correlated negatively with platelet count. In TH the positive correlation between the blood pressure and the (p.c.) of CD54 peripheral blood lymphocytes as well as CD54 MFI on decidual lymphocytes were found. Conclusions: (1) PIH, especially PE, is accompanied by overexpression of intercellular adhesion molecule-1 (ICAM-1) on peripheral blood and decidual lymphocytes studied "in vitro", (2) Some of the parameters studied seem to correlate with clinical markers of PIH intensity but this fact needs further investigations using larger subject groups.

142/2523

Tytuł oryginału: A multicentre randomized/controlled trial of a conventional versus modestly accelerated radiotherapy in the laryngeal cancer: influence of a 1 week shortening overall time.
Autorzy: Hliniak A., Gwiazdowska B., Szutkowski Z., Kraszewska E., Kukolowicz P., Jarząbski A., Sochacka B., Mazurkiewicz M., Paprota K., Oliskiewicz W., Zadrożna O., Milecki P., Kubiak M., Czopkiewicz L., Jagas M., Góźdź S., Wieczorek A., Woytowicz A., Cisowska B., Magdziarz H., Nowakowski S., Kośniewski W., Laskosz I., Serafin A., Gradoń E.
Źródło: Radiother. Oncol. 2002: 62 (1) s.1-10, il., tab., bibliogr. 13 poz.
Sygnatura GBL: 312,846

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background and purpose: To compare in a phase III study the loco-regional control, disease-free survival and overall survival induced by an accelerated regimen (AF) as compared with conventional regimen (CF) and to analyze the early and late post-radiation morbidity in both arms. Materials and methods: Patietns with ó 75, WHO 0-1, suitable for a radical course of radiotherapy T1-T3, NO, MO, stage of glottic and supraglottic laryngeal cancer were randomized to either CF: 66Gy given in 33 fractions over 45 days or AF: 66Gy given in 33 fractions over 38 days (2 fractions every Thrusday). A total of 395 patients were included from 05.1995 to 12.1998. Results. Early toxicity: At the end of radiotherapy patietns treated with AF complained for more severe reactions than patients treated with CF. In 8 weeks after treatment completion patients treated with AF complained only for more severe pain on swallowing (P = 0.027). In 4 months after treatment completion all types of toxicity except for skin teleangiectasia (P = 0.001) were similar in the two groups. Locoregional control: comparison between CF and AF showed no difference in terms of loco-regional control (P = 0.37). Conclusions: The improvement in AF in terms of loco-regional control is estimated to be 3-5 p.c. in comparison with conventional regimen and is not significant. The intensity of reactions after 4 months was similar in both arms, what suggests the possibility of further shortening of the overall time by few days or enhancing the total dose within the limits of acceptable morbidity.

143/2523

Tytuł oryginału: The pathomechanism of posttraumatic edema of lower limbs. [P.] 1: The effect of extravasated blood, bone marrow cells, and bacterial colonization on tissues, lymphatics and lymph nodes.
Autorzy: Szczęsny Grzegorz, Olszewski Waldemar L.
Źródło: J. Trauma 2002: 52 (2) s.315-322, il., bibliogr. 31 poz.
Sygnatura GBL: 305,046

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

psy

in vitro

Streszczenie angielskie: Bacground: The mechanical injury of soft tissues and bones of lower extremities is frequently followed by long-lasting edema at the site of trauma and distally. The pathomechanisms of this complication remains unclear. Venous thrombosis and interruption of lymphatics are considered to be the main etiologic factors. We propose a concept that protracted healing of injured tissues and bones with involvement of the regional lymphatic system (lymphatics and nodes) is responsible for persistence of edema. The events affecting the first (scavenging) phase of healing of traumatized tissues, such as hematoma, translocation of bone marrow cells to soft tissues, and colonization by microorganisms, and in particular their effects on lymphatics and lymph nodes, were studied. Methods: Mongrel dogs weighing 15-20 kg were. Fresh blood or bone marrow cell (BMC) suspension was injected subcutaneously or intralymphatically into the paw. Strains of saprophyte bacteria residing on the skin surface were cultured and injected intradermally. Oilcontrast lymphography was performed before and after injections to evaluate the changes in lymphatics and nodes. Biopsy samples of paw skin, subcutaneous tissue, and regional lymph nodes (LN) were taken. The responsiveness of LN lymphocytes was studied in autologous mixed cultures with peripheral blood lymphocytes (PBL), BMC, and cultured bacteria. Results: The PBL from subcutaneously injected blood were evacuated by the lymphatic route at a rate of 1-3 proc./6 hr. There was no thrombosis of lymphatic vessels or obstruction of LN sinusoids...

144/2523

Tytuł oryginału: Long-term changes in calbindin D28K immunoreactivity in the rat hippocampus after cardiac arrest.
Autorzy: Sadowski Marcin, Lazarewicz Jerzy W., Jakubowska-Sadowska Katarzyna, Wiśniewski Henryk M., Mossakowski Mirosław J., Brown W. Ted
Źródło: Neurosci. Lett. 2002: 321 (1/2) s.90-94, il., bibliogr. 20 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Calbindin D28K (CB) expression was analyzed in the rat hippocampus following 10-min-cardiac arrest-induced ischemia within a year after reperfusion. In rats examined 3 days after ischemia, CB immunoreactivity disappeared completely from CA1 pyramidal neurons and from most CA2 pyramids. In the stratum granulosum of the dentate gyrus, mossy fibers, and hippocampal interneurons, CB immunoreactively was preserved, although staining was somewhat paler than that in control rats. A similar pattern of CB immunoreactively was found in rats sacrificed 14 days and 1 month after cardiac arrest. From the 14th postischemic day, neuronal loss in the stratum pyramidale of CA1 but not in that of CA2 became apparent. The reappearance of CB immunoreactivity in CA1 and CA2 pyramidal neurons was noticed 6 months after ischemia, and the pattern was identical to that observed in animals sacrificed 12 months after the octus. The prolonged loss and delayed reappearance of CB immunoreactivity in the hippocampus demonstrate that ischemia may induce long-term disturbances of protein expression, which may in turn result in impairment of hippocampal functioning.

145/2523

Tytuł oryginału: Induction of oxidative DNA damage by carcinogenic metals.
Autorzy: Bal Wojciech, Kasprzak Kazimierz S.
Źródło: Toxicol. Lett. 2002: 127 (1/3) s.55-62, bibliogr. s. 60-62
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The metal ions carcinogenic to humans are As, Be, Cd, Cr and Ni, and the candidates also include Co, Cu, Fe and Pt. A range of molecular mechanisms was proposed for these metals, reflecting their diverse chemical properties. The oxidative concept in metal carcinogenesis proposes that some complexes of the above metals (Co, Cr, Cu, Fe, Ni) formed in vivo undergo redox cycling, yielding reactive oxygen species and/or high valence metal ions which oxidize DNA. Some of the products of oxidative DNA damage, including 8-oxoguanine and strand breaks, induce mutations, which may lead to neoplastic transformation. The establishment of metal-binding modes in the cell nucleus and of their reactivity is crucial for the understanding of molecular events in metal carcinogenesis. We have proposed the binding sites for Ni(II) and Cu(II) in core histones (H3, H2A) and sperm protamines (HP2) and, using molecular models, provided evidence for the generation of promutagenic oxidative DNA damage by the bound metals.

146/2523

Tytuł oryginału: Recent advances in understanding of the molecular basis of anhidrotic ectodermal dysplasia: discovery of a ligand, ectodysplasin A and its two receptors.
Autorzy: Wiśniewski Sławomir A., Kobielak Agnieszka, Trzeciak Wisław H., Kobielak Krzysztof
Źródło: J. Appl. Genet. 2002: 43 (1) s.97-107, il., tab., bibliogr. (42) poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Recent developments of the investigations on the molecular basis of anhidrotic ectodermal dysplasia are reviewed. Identification of the major product of the EDA gene (ectodysplasin A), a protein belonging to a group of TNF ligands, and molecular cloning of the cDNA, encoding its receptor (EDAR), a member of the RNF receptor family, are presented. The role of an alternative EDA receptor, localised on the X chromosome (XEDAR) in the development control of the differentiation of skin appendages, is discussed. Recent findings ave elucidated the cause of the autosomal forms of EDA, both dominant and recessive, and indicated an important role of a signal transduction pathway involving a protein product of the NEMO gene and the transcription factor NFkB in the differentiation of skin appendages.

147/2523

Tytuł oryginału: Transition C2718T in the AR gene, resulting in generation of a termination codon and truncated form of the androgen receptor, causes complete androgen insensitivity syndrome.
Autorzy: Ignacak Monika, Niedziela Marek, Trzeciak Wiesław H.
Źródło: J. Appl. Genet. 2002: 43 (1) s.109-114, il., tab., bibliogr. (14) poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The action of testosterone and 5ŕ-dihydrotestosterone are essentail to the development of the male phenotype. Patients with karyotype 46,XY, resistant to these hormones, exhibit a wide spectrum of phenotypes: from phenotypic female, through a range of incomplete masculinization, to under-virilized, infertile man. Theses disturbances are caused by mutations in the androgen receptor gene (AR). We studied a 46,XY fenotypic female with typical symptoms of Complete Androgen Insensitivity Syndrome (CAIS). Multiple temperature single-stranded confirmation polymorphism (MSSCP) an sequence analysis of exon 6 of the AR gene in a patient revealed a C2718T transistion causing R786X mutation in the loop between helices VII and VIII of the LBD of the androgen receptor. The R786X mutation has been described in a patient with CAIS only once and no such mutations have been described in Eastern Europe.

148/2523

Tytuł oryginału: Trisomy 8 mosaicism syndrome.
Autorzy: Wiśniewska Marzena, Mazurek Małgorzata
Źródło: J. Appl. Genet. 2002: 43 (1) s.115-118, il., bibliogr. (7) poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Authors present the case of a 15-year-old boy assessed for Marfan syndrome for many years. The child was treated because of skeletal defects, mild mental deficiency and dysmorphic features of face. Chromosomal analysis showed a trisomy 8 mosaicism.

149/2523

Tytuł oryginału: Mirosław J. Mossakowski in memoriam [1929-2001].
Autorzy: Albrecht Jan
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.I-II, il.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Mossakowski

Mirosław Jan 1929-2001

150/2523

Tytuł oryginału: Proteolysis of endomorphin-1 by brain synaptic membrane-associated peptidases.
Autorzy: Wu Liming, Chen Junhui, Zhong Dulai, Lin Chentao, Wang Xingchang
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.1-5, il., bibliogr. [15] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The biotransformation of endomorphin-1 (EM-1) by brain synaptic membranes has ben studied. Peptide fragments of EM-1 that were formed during incubation with membrane preparations in vitro were isolated by High-performance liquid chromatography (HPLC) and characterized using determination of amono acid composition. At pH 7.4, two degradation products, EM-1(1-3) and EM-1(1-2), were identified. EM-1 was degraded 77.5 p.c. at 30 min incubation with synaptic membranes. The time course of the experiments and the effect of carboxypeptidase inhibitor (CPI) demonstrated that the proteolysis reaction involves the participation of carboxypeptidase activity.

151/2523

Tytuł oryginału: The time course of the last contractions during incompletely fused tetani of motor units in rat skeletal muscle.
Autorzy: Celichowski Jan, Bichler Edyta
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.7-17, il., tab., bibliogr. [42] poz.
Sygnatura GBL: 302,090

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The time course of the contraction and the relaxation of individual contractions during incompletely fused tetani of motor units were analyzed. Investigations were performed on fast fatigable (FF), fast resistant (FR) and slow (S) motor units of the rat medial gastrocnemius muscle. Stimulation of a motor unit with a series of nine trains of stimuli at a frequency from 10 to 150 Hz was used and tetani fused to a variable degree were recorded. For fast motor units the procedure was repeated twice and observations were made on potentiated tetani in the second series of stimulation. For each tetanus, the amplitude of the tension increase, the peak amplitude of the contraction, the contraction time and the half-relaxation time were measured in the last contraction of the tension recording. It was observed in all three types of motor units that the last contraction was prolonged in parallel with the increase of fusion of a tetanus. In this contraction, the contraction time slightly decreased whereas the half-relaxation time strongly prolonged. The prolongation of the half-relaxation time was the strongest in tetani of slow units. Moreover, for the last contraction in a tetanus, the rate of changes in tension were studied. The rate of increase in tension during the contraction decreased in parallel with the increase of fusion of a teatanus, whereas the maximal rate of the tension decrease during the relaxation was found in tetani with fusion indices of 0.79, 0.98 and 0.95 for FF, FR and S motor units, respectively. Changes in the time course of contractions in tetani to a variable degree can shed light on processes of summation of contractions in unfused tetani at the level of individual motor units.

152/2523

Tytuł oryginału: Superior laryngeal nerve section abolishes capsaicin evoked chemoreflex in anaesthetized rats.
Autorzy: Kaczyńska Katarzyna, Szereda-Przestaszewska Małgorzata
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.19-24, il., bibliogr. [21] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Respiratory effect of an intravenous injection of capsicin were investigated in nine vagotomized and subsequently laryngeally deafferentated, urethane- and chloralose- anaesthetized and spontaneously breathing rats. Bolus injection of capsaicin (5 ćg/kg) into the right femoral vein induced an expiratory apnoea of 4.23 ń 0.63 s duration (mean ń SEM). In post-apnoeic breathing, tidal volume increased by 14 p.c. from the control level (P 0.05) in all nine rats treated by vagotomy. Section of the superior laryngeal nerves (SLNs) precluded the occurrence of apnoea. Results of this study indicate that in vagotomized rats sensory input from the larynx constitute an important pathway to the nodose ganglia endowed with capsaicin receptors.

153/2523

Tytuł oryginału: Effects of social rearing conditions on conditioned suppression in rats.
Autorzy: Walasek Grażyna, Węsierska Małgorzata, Werka Tomasz
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.25-31, il., bibliogr. [34] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: mwenty-two rats were reared in standard conditions during the first two months of their life. Then the animals were divided into two groups exposed to different rearing conditions. Twelve animals (Group SO) were housed socially, six animals per cage, and for three weeks they were subjected to sensory stimulation in an enriched environment. The other ten subjects were kept individually (Group IN); one rat per mesh cage, in conditions of relatively impoverished sensory stimulation. In both groups the training of the conditioned emotional response (CER) was performed when animals were three months old. In contrast to IN subjects, the rats subjected to permanent social contacts and reared in the enriched environment (Group SO) revealed almost equally low instrumental response rates in trials with the conditioned stimulus (CS) paired with nociceptive foot-shock (US), and in period when no CS and/or US were applied. The results suggested that early exposure to an enriched environment caused a later decrease of the animals' capability to differentiate between the aversive CS and cues of the experimental context. This cognitive impairment was probably a secondary efffect of fear generalized to the entire experimental situation.

154/2523

Tytuł oryginału: Perception of facial affect in chronic schizophrenia and right brain damage.
Autorzy: Kucharska-Pietura Katarzyna, Klimkowski Marceli
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.33-43, il., tab., bibliogr. [52] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: This study was designed to compare the performance of 50 chronic schizophrenics (CS) to that of 30 right brain-damaged patients (R), and 50 healthy controls (N) on several facial perception measures: Emotion Labelling and Recognition, and the Benton Facial Recognition Test. CSs were diagnosed according to DSM-IV criteria and their psychiatric state was assessed using the PANSS scale. All subjects were right handed. Their cognitive state was assessed using the MMSE. Subjects rated their current mood on a visual analogue scale. The results showed that the CSn and Rs were significantly impaired compared to Ns for the emotional tasks but did not differ from each other. Moreover, the patient groups were significantly less accurate in recognising emotionally neutral facial stimuli. Each subject group had more difficulty processing negative relative to positive affect. The deficit in schizophrenia was found to be stable, which may reflect a trait-like, rather than a state-dependent, characteristic. Moreover, some support is provided for the notion that facial affect perception in chronic schizophrenia is associated with right hemisphere dysfunction.

155/2523

Tytuł oryginału: Hypoxic damage of the cerebellum in 7-day-old rats. Ultrastructural and histochemical study.
Autorzy: Dąmbska Maria, Gajkowska Barbara
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.45-49, il., bibliogr. [15] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The damaging influence of hypoxia on the cerebellum in immature rats, which is still discussed, was investigated. Using material obtained in a modified Levine model for combined hypoxic-ischemic damage in 7-day-old rats, we examined changes in cerebellum submitted to hypoxia only. The results demonstrated classic features of hypoxic nervous tissue damage and calcium accumulation in mitochondria and endoplasmic reticulum. This was investigated using electron microscopy combined with the oxalate-pyroantimonate method. We propose that Caý+ increases in endoplasmic reticulum and mitochondrial Caý+ pools may be involved in damage-mediated mechanisms. These results support a role of calcium as a mechanism of cerebellar cell loss after this form of injury.

156/2523

Tytuł oryginału: Centrally applied vasopressin prevents posthemorrhagic hypotension in WKY rats.
Autorzy: Ufnal Marcin, Żera Tymoteusz
Źródło: Acta Neurobiol. Exp. 2002: 62 (1) s.51, il.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

157/2523

Tytuł oryginału: Serum concentrations of markers of type I collagen metabolism in women taking monophasic oral contraceptives.
Autorzy: Rechberger Tomasz, Baranowski Włodzimierz, Bogusiewicz Michał, Postawski Krzysztof, Jakimiuk Artur J., Tomaszewski Jacek, Kulik-Rechberger Beata, Jakowicki Jerzy A.
Źródło: Ginekol. Pol. 2002: 73 (2) s.81-86, il., tab., bibliogr. 21 poz., streszcz.
Sygnatura GBL: 301,184

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Karboksyterminalny propeptyd kolagenu typu I (PICP-carboxy-terminal propeptide of type I procollagen) i stabilizowany pirydynoliną karoboksyterminalny telopeptyd kolagenu typu I (ICTP - cross-linked carboxy-terminal telopeptide of type I collagen) są markerami odpowiednio rozpadu i synetzy kolagenu typu I. Ich stężenia w surowicy krwi odzwierciedlają tempo przemian tego białka w organizmie. Oznaczono stężenia PICP i ICTP w surowicy kobiet przyjmujących doustne środki antykoncepcyjne zawierające 20 ćg etinylestradiolu i 150 ćg desogestrelu (Mercilon) lub 30 ćg etinylestradiolu i 150ćg desogestrelu (Marvelon) przez 6 miesięcy. Stwierdzono znamiennie niższe średnie stężenie PICP po 3 miesiącach przyjmowania preparatu zawierającego 20 ćg etinylestradiolu i 150 ćg desogestrelu, które jednak po 6 miesiącach nie różniło się istotnie od wartości poczatkowej. U kobiet przyjmujących preparat zawierający 30 ćg etinylestradiolu i 150 ćg desogestrelu nie zaobserwowano znaczących zmian stężenia PICP. Średnie stężenia ICTP nie uległy istotnym zmianom w obu grupach. Wyniki wskazują na przemijające zmniejszenie syntezy kolagenu typu I u kobiet przyjmujących środki antykoncepcyjne zawierające 20 ćg etinylestradiolu. Żaden z obu badanych preparatów nie wpływa znacząco na degradację kolagenu typu I w organizmie kobiet podczas pierwszych sześciu miesięcy przyjmowania.

Streszczenie angielskie: Objectives: To investigate serum concentrations of the carboxy-terminal of type I procollagen (PICP) and cross-linked carboxy-terminal telopeptide of type I collagen (ICTP), reflecting the rate of synthesis and degradation of the parent collagen respectively, in women using monophasic oral contraceptives. Study design: PICP and ICTP were estimated in 60 women taking 20 ćg of ethinyl estradiol (EE) + 150 ćg of desogestrel (DSG) or 30 ćg of EE + 150 ćg DSG over six months. Results: Mean PICP concentration decreased in women receiving 20 ćg of EE + 150 ćg of DSG after three cycles of administration. However, after six months this value returned to a leel comparable to the initial one. In women taking 30 ćg of EE + 150 ćg of DSG the mean concentrations of PICP did not change significantly throughout the period studied. None of the oral contraceptives noticeably influenced the concentrations of ICTP. Conclusion: Investigated oral contraceptives do not significantly affect the metabolism of type I collagen, however a transient decrease in its biosynthesis may be expected during the use of formulations containing 20 ćg of EE.

158/2523

Tytuł oryginału: Evaluation of the antisperm antibodies in infertile couples afflicted with a cervical factor.
Autorzy: Rogoza Andrzej, Łukaszuk Krzysztof, Mielcarek Piotr
Źródło: Ginekol. Pol. 2002: 73 (2) s.87-92, il., bibliogr. 11 poz., streszcz.
Sygnatura GBL: 301,184

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Dokonano oceny metod stosowanych w wykrywaniu przeciwciał przeciwplemnikowych w diagnostyce niepłodności immunologicznej. Materiał stanowiło 38 par leczonych w II Klinice Położnictwa i Ginekologii AMG z powodu podejrzenia niepłodności immunologicznej. Na podstawie przeprowadzonych badań stwierdzono małą przydatność diagnostyczną lateksowego testu aglutynacji. Jako podstawową metodę wykrywania przeciwciał przeciwplemnikowych proponujemy metodę Western blottingu.

Streszczenie angielskie: Objectives: The purpose of this study was to evaluate methods used for antisperm antibodies detection in infertility. Methods: The studied cohort comprised 38 infertile couples with a distinct cervical factor. Presence of antisperm antibodies and their levels in circulation were evaluated in sera samples of both partners and also in the cervical mucus and semen with the Latex Agglutination test. Western Blotting was applied as an additional method in antibody detection. We also assessed: the number of sexual partners, potentially allergizing sexual behaviour and other potentially sensitising factors. Results: The positive antisperm antibodies were detected merely twice and only in one case there was evidence of insemination-impeding antisperm humoral response. The Western Blotting method enabled us to obtain a reaction to a range of sperm proteins which reaction to a range of sperm protein which reacted with antibodies both in serum and in seminal plasma. Conclucions: Determination of infertility on immunological grounds on the basis of a single determinant on sperm presents little diagnostic value. In our view, the combination of patient's clinical status with immune-system response to a spectrum of sperm antigens provides means of infertility evaluation. We propose Western Blotting as an useful technique for detection of antisperm antibodies.

159/2523

Tytuł oryginału: Novye struktury 0-cpecificheskikh polisakharidov bakterijj roda Proteus. 2: Polisakharidy, soderzhashhie O-acetilnye gruppy.
Tytuł angielski: New structures of the O-specific polysaccharides of Proteus. [P.] 2: Polysaccharides containing O-acetyl groups.
Autorzy: Kondakova A. N., Toukach F. V., Senchenkova S. N., Arbatsky N. P., Shashkov A. S., Knirel Y. Ju., Zych K., Torzewska A., Rożalski A., Kołodziejska K., Sidorczyk Z.
Źródło: Biokhimija 2002: 67 (2) s.240-252, il., tab., bibliogr. 36 poz., sum.

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Structures of five new O-specific polysaccharides of the lipopolysaccharides of bacteria Proteus were established. Four of them, Proteus penneri 4 (072), Proteus vulgaris 63/57 (037), Proteus mirabilis TG 277 (069), and Proteus penneri 20 (017), contain O-acetyl groups in non-stoichiometric quantities, and the polysaccharide of P. penneri 1 st structuraly related to that of P. penneri 4. The structures were elucidated using NMR spectroscopy, including one-dimensional 1H nd 13C NMR, two-dimensional 1H, 1H correlation (COSY, TOCSY), 1H, 13C heteronuclear multiple-quantum coherence (HMQC) and multiple-bond correlation spectroscopy, as well as nuclear Overhauser effect spectroscopy (NOESY or ROESY), along with chemical methods. The structural data obtained are useful as the chemical basis for the creation of the classification scheme for Proteus strains.

160/2523

Tytuł oryginału: Novye struktury O-specificheskikh polisakharidov bakterijj roda Proteus. 1: Fosfatsoderzhashhie polisakharidy.
Tytuł angielski: New structures of the O-specific polysacchardies of Proteus. [P.] 1: Phosphate-containing polysaccharides.
Autorzy: Toukach F. V., Kondakova A. N., Arbatsky N. P., Senchenkova S. N., Shashkov A. S., Knirel Y. A., Zych K., Rożalski A., Sidorczyk Z.
Źródło: Biokhimija 2002: 67 (2) s.318-331, il., tab., bibliogr. 23 poz., sum.

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The O-specific polysaccharide chains (O-antigens) of the lipopolysacchardides of five Proteus strains, P. vulgaris 017, P. mirabilis 016 and 033, and P. penneri 31 and 103, were found to contain phosphate groups that link the non-sugar components, namely, ethanolamine and ribitol. From them, the polysaccharides of P. mirabilis 016 and P. penneri 103 include ribitol phosphate in the main chain and thus resemble ribitol teichoic acids of Gram-positive bacteria. The structures of the polysaccharides were elucidated using NMR spectroscopy, including two-dimensional 1H, 1H correlation spectroscopy (COSY and TOCSY), nuclear Overhauser effect spectroscopy (NOESY or ROESY), and H-detected 1H, 13C, and 1H, 31P heteronuclear multiple-quantum coherence (HMQC), along with chemical methods. The structures determined are unique among the bacterial polysaccharides and, together with data obtained earlier, represent the chemical basic for classification of Proteus strains. Based on structural similarities of the O-specific polysaccharides and serological relationships between the O-antigens, we propose to add P. penneri strain 31 to serogroup 019 and to extend serogroup 017 by including P. penneri strain 16.

161/2523

Tytuł oryginału: Further studies on the anti-oxidative effect of putrescine in sodium nitrite-treated rats.
Tytuł polski: Dalsze badania nad antyoksydacyjnym wpływem putrescyny u szczurów zatruwanych azotynem sodowym.
Autorzy: Grudziński Ireneusz P., Frankiewicz-Jóźko Anna
Źródło: Rocz. Państ. Zakł. Hig. 2002: 53 (1) s.11-17, il., bibliogr. 30 poz., streszcz.
Sygnatura GBL: 301,906

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie polskie: Badano antyoksydacyjne właściwości putrescyny (10 mg/kg m.c./dzień) u szczurów zatruwanych per os azotynem sodowym (10 mg NaNO2/kg m.c./dzień) przez okres 14 dni. Putrescynę podawano per os w 3-4 godz. po podaniu azotynu i/lub 0,9 p.c. NaCl (kontrola) przez okres 7 dni (dzień 7-14). Azotyn sodowy zwiększył poziom substancji reagujących z kwasem tiobarbiturowym (TBARS) w błonie śluzowej jelita cienkiego i wątrobie, nie wpływając na zmiany tego parametru we krwi szczurów. Nie wykazano, aby u zwierząt zatruwanych azotynem dochodziło do zmian zarówno stanu antyoksydacyjnego surowicy (TAS) oraz aktywności dysmutazy ponadtlenkowej (SOD) we krwi, wątrobie czy błonie śluzowej jelita cienkiego. Podawanie szczurom putrescyny zmniejszyło poziom TBARS w badanych tkankach przy zwiększonym poziomie TAS w surowicy. Putrescyna znacząco obniżyła aktywność SOD w wątrobie i krwi i nie zmnieniając aktywności tego enzymu w jelicie cienkim. Rezultaty badań sugerują, że podawanie putrescyny per os szczurom może prowadzić do obniżenia peroksydacji lipidowej indukowanej azotynem sodowym.

Streszczenie angielskie: Studies were carried out to elucidate the anti-oxidative effect(s) of putrescine (10 mg/kg b.w./day) in rats treated per os with either sodium nitrite (10 mg/kg b.w./day) or normal saline (control) for 14 days. The putrescine was given to rats for 7 days only (days 7-14) and it was introduced 3-4 hrs after nitrite or saline dosage. Sodium nitrite increased thiobarbituric acid reactive substances (TBARS) in rat small intestinal mucosa and liver, and the agent did not have any effect on the total anti-oxidant status (TAS) and lipid peroxidation of rat blood. Nitrite did not also change the activity of superoxide dismutase (SOD) in the small intestinal mucosa, liver and blood, as well. Pretreatment of nitrite-treated rats with putrescine decreased TBARS and increased TAS in animals. Putrescine decreased SOD activity in the blood and liver of nitrite- and/or saline-treated rats, however, the agent did not affect the SOD enzyme in the small intestinal mucosa. Results suggest that putrescine dosed to nitrite-treated rats possesses some anti-oxidative properties.

162/2523

Tytuł oryginału: A pilot study on the histochemical evaluation of Na/K-ATPase and succinate dehydrogenase in rat intestinal villi.
Tytuł polski: Badania pilotowe nad histochemiczną oceną ATPazy-Na/K oraz dehydrogenazy bursztynianowej w kosmkach jelita cienkiego szczura.
Autorzy: Grudziński Ireneusz P.
Źródło: Rocz. Państ. Zakł. Hig. 2002: 53 (1) s.19-26, il., bibliogr. 31 poz., streszcz.
Sygnatura GBL: 301,906

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie polskie: Badano wpływ zatrucia azotynem sodowym na aktywność ATPazy-Na/K oraz dehydrogenazy bursztynianowej (SDH) w błonie śluzowej jelita cienkiego szczurów. Zwierzęta otrzymywały per os wodny roztwór azotynu sodowego (10 mg NaNO2/kg m.c.) i/lub 0,9 proc. roztwór chlorku sodowego (kontrola) przez okres 3 i 12 tygodni. Histoenzymatyczna analiza wykazała azotynowe obniżenie aktywności ATPazy-Na/K oraz SDH w kosmkach jelitowych ze szczególnym nasileniem wpływu azotynu w 12 tygodniu zatrucia zwierząt.

Streszczenie angielskie: Effects of poisoning with sodium nitrite on Na/K-ATPase and succinate dehydrogenase (SDH) activity were examined in the small intestine mucosa of male Wistar rats. The animals were treated per os with either an aqueous solution of sodium nitrite (10 mg NaNO2/kg b.w.) or normal saline (control) daily for 3 or 12 weeks. Histochemical analyses show that sodium nitrite decreased Na/K-ATPase and SDH in rat intestinal villi, and the nitrite side effect(s) was mainly observed in animals treated for 12 weeks.

163/2523

Tytuł oryginału: CD45 in memory and disease.
Autorzy: Tchilian Elma Z., Beverley Peter C. L.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.85-93, il., bibliogr. 72 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: CD45 (the leukocyte common antigen) is known to function as a tyrosine phosphatase in leukocyte signaling. Biochemical studies indicate that CD45 is involved in the regulation both of T cell receptor-associated kinases and Janus kinases that transmit signals from cytokine receptors. However, the function of the different isoforms of CD45 generated by complex alternative splicing, and indeed the role of the whole extracellular domain of the molecule, remain mysterious. Analysis of CD45 knock-outs and of trangenic mice expressing single CD45 isoforms, as well as the disease associations of human polymorphisms, is providing new insights into CD45 function. Accumulating data from these genetic and biochemical studies promises to elucidate the role of high and low molecular weight isoforms of CD45 in the function of naive and memory T lymphocytes.

164/2523

Tytuł oryginału: Low-molecular-weight protein tyrosine phosphatase and human disease: in search of biochemical mechanisms.
Autorzy: Bottini Nunzio, Bottini Egidio, Gloria-Bottini Fulvia, Mustelin Tomas
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.95-104, tab., bibliogr., 80 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: A major challenge in the post-genomic era is to identify the physiological functions of genes and elucidate the molecular basis for human disease. Genetic polymorphisms offer a convenient avenue for these efforts by providing evidence for the involvement of a given gene in human pathophysiology. Here we review the current evidence linking the low-molecular weight protein tyrosine phosphatase (LMPTP) to several common diseases, including allergy, asthma, obesity, myocardial hypertrophy, and Alzheimer's disease. Based on the known effects of the genetic polymorphisms on the alternative mRNA splicing and enzyme levels of LMPTP, we discuss the possible molecular mechanisms of LMPTP ivolvement in these diseases.

165/2523

Tytuł oryginału: Archivum Immunologiae et Therapiae Experimentalis!
Tytuł angielski: Significance of tumor-cell receptors in human cancer.
Autorzy: Żeromski Jan
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.105-110, il., bibliogr. 48 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Every tumor cell is equipped with an array of biologically active surface molecules, and several of these function as receptors for various ligands. They include MHC, or in the case of humans, HLA antigens, cytokine receptors, cell-adhesion molecules, growth factor receptors, Fas/Fas-ligand molecules and others. Their expressions are a subject to alternations, usually to the advantage of tumor growth and spread. Some appear on tumor cells de novo, having no counterparts on the respective normal cells. Detailed knowledge about the expression of tumor-cell receptors and their genotypes, in particular of cancerous ones, may provide information essential for the creation of tools for specific tumor immunotherapy.

166/2523

Tytuł oryginału: Potential for immune reconstitution through G-CSF treatment of HIV patients.
Autorzy: Aulock Sonja von, Hartung Thomas
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.111-120, il., bibliogr. 90 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: New treatment strategies for HIV/AIDS are very successful in reducing viral load. However, reconstitution of the immune system takes about one year and may be insufficient or remain incomplete. During this time the patient remains prone to opportunistic infections as a result of the complex immune dysfunction caused by the virus. Recombinant granulocyte colony-stimulating factor (G-CSF) has diverse immunomodulatory properties which may be beneficial in aiding immune reconstitution.

167/2523

Tytuł oryginału: GM-CSF regulation in eosinophils.
Autorzy: Esnault Stphane, Malter James S.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.121-130, bibliogr. 113 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Allergic asthma is characterized by a temporally and quantitatively inappropriate immunologic response. One of the hallmarks of this response is the accumulation of eosinophils in the airway and lung parenchyma, which results in broncho-constriction, lung damage and, ultimately, fibrosis. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a pivotal role in this process by modulating eosinophil function and survival. In this review, we discuss the effects and molecular regulation of GM-CSF secretion by eosinophils. Recent data demonstrate that activated eosinophils release small amounts of anti-apoptotic GM-CSF by stabilizingits coding mRNA.

168/2523

Tytuł oryginału: Immunomodulation of macrophages by pathogenic Yersinia species.
Autorzy: Ruckdeschel Klaus
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.131-137, il., bibliogr. 37 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The interaction between macrophages and bacterial pathogens plays a crucial role in the pathogenesis of infectious diseases. Pathogenic species of the Gram-negative bacterium Yersinia deploy complex strategies to disarm macrophages and to disrupt their response to infection. For this purpose, Yersinia sp. engage a type III protein secretion system that mediates the polarized translocation of Yersinia virulence factors, the so-called Yops (Yersinia outer proteins), into the host cell cytoplasm. There, the Yops act on different cellular levels to neutralize a sequence of programmed phagocyte effector functions. Yersiniae initially impair the phagocytic machinery and block the generation of th ebacterial oxidative burst. Furthermore, yersiniae uncouple an array of fine-tuned signals of innate immunity, which leads to suppression of macro\hage TNF-ŕ production and to macrophage apoptosis. The impairment of cellular functions results in a scenario by which Yersinia efficiently resists the attack of the macrophage and finally kills the macrophage by activating its intrinsic cell suicide mechanism. This review highlights the aspects of Yersinia-macrophage interaction that determine the fate of the infected cell.

169/2523

Tytuł oryginału: Effect of IL-18 on IL-1á and sIL-1RII production.
Autorzy: Jabłońska Ewa, Iżycka Aneta, Wawrusiewicz Natalia
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.139-141, il., bibliogr. 7 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In the present study we investigated the effect of interleukin 18 (IL-18) on th e production of IL-1ánd soluble IL-1 receptor II (sIL-1RII) by human neutrophils. The results obtained indicate that recombinant human IL-18 (rhIL-18) induces IL-1á and, to a lesser extent, sIL-1RII production by human neutrophil isolated from peripheral blood. However, this effect was less important than lipopolysaccharide (LPS) stimulation. Additionally, our observations suggest that IL-18 can induce priming of neutrophils for IL-1á and, to a lesser extent, sIL-1RII production by LPS-stimulated cells. The ability of IL-18 to serve as an effective modulator for IL-1áand its regulatory protein may have sigificance in the inflammatory and immune reactions mediated by IL-1á.

170/2523

Tytuł oryginału: A study of thyroglobulin concentration in the thyroid and serum of patients with different thyroid disorders.
Autorzy: Czarnywojtek Agata, Krysińska Iwona, Łącka Katarzyna, Stawny Bolesław, Rólski Michał, Jarząb Barbara, Włoch Jan, Gembicki Maciej
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (2) s.143-148, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Knowledge concerning the structure and quality of thyroglobulin (tg) has great significance for the better understanding of the pathogenesis of different thyroid diseases. The localization of the Tg gene and studies of its structure by molecular biological techniques make possible precise investigations of its expression. The aim of our study was to evaluate Tg content in the thyroids and Tg concentrations in the serum of 108 patients suffering from beenign or malignant thyroid disorders. The method of investigation was isolating total protein from thyroid tissues obtained during surgery and determining Tg content in the thyroid extracts and Tg concentrations in serum The Tg concentrations in serum and in thyroid protein extracts were evaluated by fluoroimmunometric assay. Statisctical analysis was carried out with the help of the computing programmes.

171/2523

Tytuł oryginału: Single photon emission tomography in radioimmune imaging of diabetic foot infection.
Autorzy: Bohchelian H. A., Klisarova A. D., Koeva L. A.
Źródło: Diabetol. Pol. 2002: 9 (1) s.39-44, il., tab., bibliogr. 24 poz., sum.
Sygnatura GBL: 313,197

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of our study was to evaluate the clinical efficacy of single photon emission computer tompgraphy (SPECT) in combination with planar radioimmune and methylene-diphosphonate (MDP) - scintigraphic imaging of diabetic foot infection. The study group comprised 32 patients with type 1 and type 2 diabetes mellitus. All patients had diabetic foot ulceration with possible infection. All patients underwent clinical, bacteriological, X-ray and radioisotopic examination. Biochemical and instrumental investigation was carried out to estimate the degree of glycaemic control and available chronic diabetic complications. X-ray examination included radiographs centered on the foot lesion. All patients underwent bone scintigraphy with 370 MBq Technetium-99m-MDP (Tc-99m-MDP) three hours after injection in 3 projections (AP; LAO; RAO). Radioimmune planar imaging was performed by immunoscintigraphy with Tc-99m conjugated with monoclonal antigranulocyte antibody (MAB) BW 250/183. Immunoscintigraphy was performed 4 and 24 hours after administration of 740 MBq Tc-99m-MAB BW 250/183. In positive planar immunoscintigraphic cases and necessity of precise localization of inflammatory process - single photon emission computer tomography was performed. Final diagnosis - osteomyelitis was verified by bacteriological cultures and a long term follow up. Twenty two true positive cases, eight true negative cases, one false positive case, one false negative case were established. The sensitivity of the applied method for detection of osteomyelitis was 94 p.c., specificity 92 p.c., diagnostic accracy 93 p.c...

172/2523

Tytuł oryginału: Nefiracetam ameliorates associative learning impairment in the scopolamine-injected older rabbit.
Autorzy: Pak Jonathan T., Green John T., Heifets Boris, Pak Michelle H., Woodruff-Pak Diana S.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR105-BR112, il., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The cognition-enhancing drug, nefiracetam, is in Phase III clinical trials to treate memory impairment in Alzheimer's disease (AD). Nefiracetam ameliorates acquisition of delay eyeblink classical conditioning in older rabbits, a form of associative learning with striking behavioral and neurobiological similarities in rabbits and humans. In both species, delay eyeblink conditioning engages the septo-hippocampal cholinergic system and is disrupted when the cholinergic system is antagonized. Delay eyeblink classical conditioning is impaired in normal aging and severely disrupted in AD. To test further the efficacy of nefiracetam in an animal model that mimics some of the neurobiological and behavioral effects present in AD, we tested 56 older rabbits assigned to 7 treatment groups in the 750 ms delay eyeblink conditioning procedure. Older rabbits were injected with 1.5 mg/kg scopolamine to simulate disrutpion of the cholinergic system in AD. Three doses of nefiracetam (5, 10, or 15 mg/kg) were also injected in older rabbits receiving 1.5 mg/kg scopolamine. Control groups were treated with 1.5 mg/kg scopolamine + vehicle, wehicle alone, or explicitly unpaired presentations of conditioning stimuli and vehicle or 1.5 mg/kg scopolamine + 15 mg/kg nefiracetam. Rabbits injected with 1.5 mg/kg scopolamine alone were impaired, but a dose of 15 mg/kg nefiracetam reversed significantly the behavioral impairment. Nefiracetam had ameliorating effects on a task impaired in AD in an animal model of AD: older rabbits with cholinergic system antagonism.

173/2523

Tytuł oryginału: Percutaneous implantation of a biological valve in the aorta to treat aortic valve insufficiency - a sheep study.
Autorzy: Boudjemline Younes, Bonnet Damien, Sidi Daniel, Bonhoeffer Philipp
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR113-BR116, il., bibliogr. 14 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Historically, the surgical implantation of a mechanical valve in the descending aorta permitted the reduction of regurgitant flow in chronic aortic insufficiency. Long term clinical results were excellent but with the development of the pump oxygenators, orthotopic valvar replacement has become the conventional treatment of aortic valve diseases. We wanted to reconsider the ectopic position in sheep using a new percutaneous technique. A biological valve harvested from a bovine jugular vein was sutured into a vascular stent. After the creation of a severe aortic insufficiency, the valved stent was percutaneously implanted according to standard stent placement techniques. Hemodynamic and angiographic evaluations were carried out during the procedure; finally, anatomic evaluation was performed. A severe aortic insufficiency was created in all animals (9/9). 3 had a single stent in the descending aorta and 3 had an addtional stent in the brachiocephalic trunk. A control group of 3 animals had no valve implantation. One sheep had an additional valve implant because one overdilated stent had become insufficient. Early evaluation of the implanted valve function confirmed the perfect competence of 9 out of 10 valved stents. However, for unknown reasons all the animals died within 24 hours after the procedure. Percutaneously implanted valves in the thoracic aorta of sheep with massive aortic insufficiency function well in the acute study. In comparison to previous surgical results, this technique might become an alternative to surgery in patients with chronic aortic insufficiency in whom perioperative risks are high.

174/2523

Tytuł oryginału: Serodiagnostic and immunoprophylactic potential of a 78kDa protein of Leishmania donovani of Indian origin.
Autorzy: Mukherjee Mandira, Bhattacharyya Anindita, Duttagupta Swadesh
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR117-BR122, il., tab., bibliogr. 35 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The serodiagnosis of visceral leishmaniasis due to Leishmania donovani using crude parasite antigen is complicated in many endemic areas by cross-reactions with serum from humans infected with other protozoan diseases. The search for pure antigens avoiding such cross-reactions is in progress. Developing a vaccine against cutaneous leishmaniasis has been much more successful than against visceral leishmaniasis. Immunoprophylactic studies using various combinations of antigens and adjuvants are also in progress, and several strategies are in use, with varying degrees of success. Promastigotes of Leishmania donovani were used. The 78kDa protein was purified by a monoclonal antibody tagged GNBr sepharose CL-4B column. The presence of the protein in both stages of the parasite and in kala-azar patient serum was analyzed by western blotting. ELISA was used for serodiagnosis and isotype analysis of antibodies produced in immunized mice. Immunoprophylactic studies were carried out based on in vitro transformation of amastigotes to promastigotes. The 78 kDa membrane protein, present in both amastigote and promastigote forms of the parasite, was purified to homogeneity. The protein was found to have serodiagnostic potential to detect kala-azar. BALB/c mice immunized with 78kDa protein revealed reduction in spleen parasitemia. Isotype profiles of antibodies produced by immunized mice showed increased production of IgG2a and decreased IgG levels. Our results demonstrated, for the first time, the serodiagnostic and immunoprophylactic use of a pure membrane protein isolated from Leishmania donovani of Indian origin...

175/2523

Tytuł oryginału: Functional maps of the junctions between interglobular contacts and active sites in glycolytic enzymes - a comparative analysis of the biochemical and structural data.
Autorzy: Torshin Ivan Y.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR123-BR135, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Oligomers and separate subunits of the glycolytic enzymes often have different catalytic properties. However, spectral data show an apparent lack of significant conformational changes during oligomerization. Since the conformation of an enzyme determines its catalytic properties, the structural mechanism(s) influencing the activity is of considerable interest. Analysis of the spatial structures of the junctions between interglobular contacts and binding sites may give a clue to the mechanism(s) of the activation. In this work, the problem was studied using available structural and biochemical data for the oligomeric enzymes of glycolysis. Computational analysis of the structures of the junctions has identified three structurally distinct types of junctions: 1. interglobular binding site (2 of 8 enzymes); 2. domain-domain stabilization (5 of 8); and 3. 'sequence overlap' or a local conformational change (all enzymes). Thus the catalytic activity may be influenced through the shifts of the modules of protein structure (types 1,2) and/or due to a slight change in the local structure (type 3). The more common junctions of types 2 and 3 are well conserved among eukaryotic enzymes, which suggests their biological importance. The results suggest that a profound and a complex change in conformation in subunits of an oligomeric enzyme may not be necessary for a significant change in the catalytic properties. The analysis maps the residues important for the junctions and thus for the link between the catalytic activity and the oligomeric state of the enzymes.

176/2523

Tytuł oryginału: Intracerebroventricular administration of bacterial lipopolysaccharide prevents the development of acute experimental pancreatitis in the rat.
Autorzy: Jaworek Jolanta, Bonior Joanna, Nawrot Katarzyna, Leja Anna, Sendur Ryszard, Stachura Jerzy, Pawlik Wiesław, Konturek Stanisław
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR136-BR143, il., tab., bibliogr. 46 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Lipopolysaccharides (LPS) are responsible for septic shock but low doses of LPS reduce pancreatic damage produced by caerulein-induced pancreatitis (CIP) in rats. Leptin, produced by adipocytes attenuates the severity of CIP. The aim of this study was to evaluate the effect of intracerebroventricular (i.c.v) administration of LPS on CIP and plasma leptin level and to investigate the involvement of sensory nerves (SN) in the effects of LPS on CIP. CIP was produced by subcutaneous (s.c.) infusion of caerulein (25 ćg/kg) to conscious rats. SN were deactivated with capsaicin (100 mg/kg s.c.). LPS (0.2, 2, or 20 ćg/rat) were applied to the right cerebral ventricle 30 min prior to CIP. CIP was manifested by an increase in plasma levels of amylase, lipase, leptin and an antiinflammatory interleukin 10 (IL-10), (by 400 p.c., 1000 p.c., 700 p.c. and 50 p.c., respectively), confirmed by histological examination and accompanied by 40 p.c. reduction in pancreatic blood flow. Pretreatment of CIP rats with i.c.v. LPS resulted in significant reduction of CIP accompanied by dose-dependent increase in plasma levels of leptin and IL-10. Deactivation of SN, which by itself failed to affect CIP, completely reversed the beneficial effects of i.c.v. administration of LPS on CIP and reduced plasma leptin and IL-10 concentrations. Pretreatment with LPS given i.c.v. prevents the development of caerulein-induced pancreatitis through the activation of SN and though the release of leptin.

177/2523

Tytuł oryginału: Fibrinogen and smooth muscle cell detection in atherosclerotic plaques from stable and unstable angina - an immunohistochemical study.
Autorzy: Loukas Marios, Dąbrowski Marek, Wagner Teresa, Walczak Ewa, Witkowski Andrzej, Rużyłło Witold
Źródło: Med. Sci. Monitor 2002: 8 (4) s.BR144-BR148, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: This study presents a systematic analysis of atherothrombotic lesions taken by percutaneous atherectomy and post mortem examination from coronary arteries, in order to identify: a) the topographic occurrence of fibrinogen and smooth muscle cells (SMCs), b) their independent expression in stable and unstable plaques, and c) their co-expression, which can provide a better understanding of the involvement of fibrinogen and SMCs in the development and progression of atherosclerotic plaques. Material/Methods: 120 specimens from atherosclerotic lesions were collected, using directional coronary atherectomy; 40 additional specimens were collected from postmortem examinations. All specimens were stained by immunohistochemical methods with monoclonal and polyclonal antibodies (DAKO) for fibrinogen and SMCs. Results: Fibrinogen appeared to be a component of all stable and unstable coronary atherosclerotic plaques, with a significant predominance in unstable angina. No significant difference was observed between SMC-stained areas in stable and unstable angina; however, a significant difference exists in co-expression of SMCs and fibrinogen between unstable and stable angina. Interestingly, the total number of SMCs at the first stages of formation of unstable plaques is less than in stable plaques. However, in a number of advanced coronary atherosclerotic plaques associated with unstable angina, we observed an increasingly progressive inflammatory cell activity, in which SMC areas were sifnigicantly increased...

178/2523

Tytuł oryginału: Effects of acute hypoxemia/ischemia on EEG and evoked responses at normothermia and hypothermia in humans.
Autorzy: Stecker Mark M., Escherich Alison, Patterson Terry, Bavaria Joseph E., Cheung Albert T.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR223-CR228, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Hypothermia is used clinically to prevent neurologic injury but the degree of protection which it affords at various levels of the nervous system in humans is difficult to establish. Material/Methods: The temporal changes in EEG amplitude and somatosensory evoked potential (SEP) amplitudes in a patient experiencing acute normothermic hypoxemia, a patient experiencing acute circulatory arrest at moderate hypothermia and a collection of patients undergoing deep hypothermic circulatory arrest were analyzed to determine the rate at which changes occur during acute lack of oxygen delivery at various temperatures. Results: In each case, it was found that more rostrally geneated potentials disappeared more quickly than more peripheral potentials. All potentials decayed more slowly during acute normothermic hypoxemia than during circualtory arrest. During circulatory arrest at 14.4řC, the amplitude of the Erb's point, 13 and N18 potentials in th SEP took 5 times longer to drop to 50 p.c. of their value at the onset of ischemia than with circulatory arrest at 30.9řC. Conclusions: The longer times to disappearance of the SEP potentials during deep hypothermia compared to moderate hypotheramia was consistent with the predicted 3.5 - 6.5 fold reduction in metabolic activity at deep hypothermia compared to moderate hypothermia. The prolonged time to disappearance of the SEP during normothermic hypoxemia demonstrates that even with reduced oxygen delivery the continued delivery of metabolic substrate can be critical to neural function.

179/2523

Tytuł oryginału: Protocol for rapid sequence intubation in pediatric patients - a four-year study.
Autorzy: Marvez-Valls Eduardo, Houry Debra, Ernst Amy A., Weiss Steven J., Killeen James
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR229-CR234, tab., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: The purpose of the study was to evaluate a protocol for rapid sequence intubation (RSI) for pediatric patients in a Level 1 trauma center. Material/Methods: Retrospective review of prospectively gathered Continuing Quality Improvement (CQI) data at an inner city Level 1 trauma center with an emergency medicine residency program. Protocols for RSI were established prior toinitiating the study. All pediatric intubations at the center from February 1996 to February 2000 were included. Statistical analysis included descriptive statistics for categorical data and Chi-square for comparisons between groups. Results: Over the 4-year study period there were 83 pediatric intubations ranging in age from 18 months to 17 years; mean age 8.6. All had data collected at the time of intubation. There were 20 (24 p.c.) females and 62 (76 p.c.) males (p 0.001). Resons for intubation were related to trauma in 71 (86 p.c.) and medical reasons in 12 (14 p.c.) (p 0.001). Of the trauma intubations 7 (10 p.c.) were for gunshot wounds, 39 (55 p.c.) were secondary to MVCs, and the remainder (25; 35 p.c.) were from assaults, falls, and closed head injuries. The non-trauma intubations were for smoke inhalation, overdose, seizure, HIV related complications, eclapsia, and near drowning. Intubations were successful with one attempt in 65 (78 p.c.) cases. No surgical airways were necessary. Rocuronium was used in 4 cases. Protocol deviations did not lead to complications. Conclusions: This protocol based pediatric rapid sequence intubation method worked well in an EM residency program. More intubations were in males and more were necessary due to trauma in this group.

180/2523

Tytuł oryginału: Side clamp used during off-pump coronary artery bypass does not increase the risk of stroke.
Autorzy: Hirose Hitoshi, Amano Atsushi, Takahashi Akihito
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR235-CR240, tab., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of this study was to investigate whether the incidence of stroke after off-pump AC bypass was lower than that after on-pump AC bypass. Material/Methods: A prospective study was performed with consecutive patients who underwent isolated AC bypass in the Shin-Tokyo Hospital Group between July 1, 1996 and December 31, 2001 AC bypass was defined as coronary artery bypass requiring proximal anastomoses onto the ascending aorta. The patietns were divided into two groups, depending on the use of cardiopulmonary bypass: on-pump and off-pump groups. Perioperative data were prospectively collected and the risk factors for postoperative stroke were investigated. Resutls: Preoperative comorbidiites were more frequent in the off-pump group. The number of distal anastomoses was not significantly different but the number of proximal anastomoses was significantly greater in the on-pump group than in the off-pump group. The incidence of postoperative stroke was 0.3 p.c. in the off-pump group and 2.0 p.c. in teh on-pump group. Significant risk factors influencing the occurrence of postoperative stroke included: use of cardiopulmonary bypass, age over 75, peripheral vascular disease, saphenous vein use, and postoperative atrial fibrillation. Multivariate analysis demonstrated that the use of cardiopulmonary bypass, age over 75, and peripheral vascular disease were isolated predictors of postoperative stroke. Conclusions: The incidence of postoperative stroke was lower in the off-pump group than in the on-pump group, even though the side clamp was applied to the ascending aorta for aortocoronary bypass. The use of side clamping for off-pump AC bypass does not increase the risk of postoperative stroke.

181/2523

Tytuł oryginału: Predictors of mortality among nursing home residents with a diagnosis of Parkinson's disease.
Autorzy: Fernandez Hubert H., Lapane Kate L.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR241-CR246, tab., bibliogr. 63 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Little is konwn about predictors of mortality among Parkinson patients living in long term care. Material/Methods: We conducted a 3-year follow-up study on 15,237 PD residents aged 65 years and older using the Systematic Assessment in Geriatric drug use via Epidemiolgoy (SAGE) database. The SAGE database consists of the Minimum Data Set (MDS) data collected on over 400,000 nursing home residents in 5 U.S. states, including demographic characteristics, demntia severity, comorbidity and other clinical variables. Information on death was derived through linkage to Medicare files. Baseline characteristics were used to predict survival using univariate and multivariate Cox proportional hazard models. Results: The overall 3-year mortality rate was 50 p.c. Advanced age (relative rate (RR) 2.22; 95 p.c. confidence interval (CI) 1.99 - 2.47, for patients 85+ years), male gender (RR 1.73; 95 p.c. CI 1.60 - 1.87), severe functional (RR 1.81; 95 p.c. CI 1.53 - 2.13) and cognitive (RR 1.54; 95 p.c. CI 1.38 - 1.72) impairment, the presence of vision problems (RR 1.25; 95 p.c. CI 1.20 - 1.57), pressure ulcers (RR 1.25; 95 p.c. 1.14 - 1.37), and a diagnosis of congestive heart failure (RR 1.49; 95 p.c. CI 1.35 - 1.65), diabetes mellitus (RR 1.22; 95 p.c. 1.11 - 1.35) and pneumonia (RR 1.39; 95 p.c. CI 1.09 - 1.77) were independent predictors of death. The specific presence of aspiration pneumonia had the highest mortality risk ratio among all comorbidities (RR 1.58; CI 0.97 - 2.56). Africa-Americans and other minority groups were less likely to die relative to whith PD residents...

182/2523

Tytuł oryginału: Causes for early reintervention after thyroidectomy.
Autorzy: Hurtado-López Luis Mauricio, Zaldivar-Ramirez Felipe Rafael, Kuba Erich Basurto, Cejudo Abraham Pulido, Flores Jose Humberto Garza, Solis Oscar Muńoz, Castillo Carlos Campos
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR247-CR250, tab., bibliogr. 12 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of our study was to ascertain the causes for early reintervention after thyroidectomy performed by a surgical team using a systematized surgical technique. Material/Methods: We analyzed 1131 patietns, 939 (83.1 p.c.) women and 192 (16.9 p.c.) men, average age 38.7 years (range 12 to 79). Of these patients, there were 675 hemithyroidectomies with isthmusectomy (59.74 p.c.), 189 subtotal thyroidectomies (16.71 p.c.), and 267 total thyoidectomies, alone or with regional lymphatic dissection at levels VI and VII (23.55 p.c.). Statistical analysis was performed by main tendency measures and chi square (chi-squared) for comparison of two independent samples; the dependent variable was the rate of early reintervention, while the independent variables included causes, time of presentation, hormonal functional state and extent of surgery. Results: Early reintervention was necessary in 11 cases (0.97 p.c.). 9 were due to hematoma (0.79 p.c.) resolved with drainage and hemostasis, and two (0.18 p.c.) due to acute respiratory failure (ARF) caused by laryngeal edema, resolved by tracheostomy. Analysis based on diagnosis, extent of surgery and functional state failed to reveal statistically significant differences. The maximum time presentation of complications was 6 hours. Conclusions: The most intense postoperative monitoring is necessary during the first six hours. The low frequency of early reintervention and the appearance of complications in less than 8 hours enable thyroid surgery to be performed on a short-stay basis with adequate safety margins.

183/2523

Tytuł oryginału: Characteristics and outcome among women and men transported by ambulance due to symptoms arousing suspicion of acute coronary syndrome.
Autorzy: Herlitz Johan, Starke Mia, Hansson Elisabeth, Ringvall Eva, Karlson Bjrn W., Waagstein Lisbeth
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR251-CR256, tab., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of this study was to describe the characteristics and outcome in relation to sex in consecutive patients who were transported by an ambulance due to symptoms arousing suspicion of acute coronary syndrome. Materia/Methods: Our reserach involved all patietns transported by ambulance over a 3-month period in the community of Gteborg due to such symptoms. The P-values were age adjusted. Results: Of the 930 transported patietns fulfilling the given criteria, 452 (49 p.c.) were of women. The women were older and had a lower incidence of previous acute myocardial infarction, angina pectoris, and current smoking. Women less frequently had a final diagnosis of acute coronary syndrome (22.3 p.c vs 36.6 p.c.; p 0.0001) or acute myocardial infarction (10.1 p.c. vs 17.9 p.c.; p 0.0001). However, the mortality rate during one year was the same in women (17.2 p.c.) and men (18.7 p.c.). Women were less frequently clammy on admission to the ambulance (17 p.c. vs 30 p.c.; p 0.0001) and less frequently showed signs of myocardial ischemia in ECG upon admission to the emergency department (26 p.c. vs 38 p.c.; p 0.001) compared to men. Among those patietns with an acute coronary syndrome, women more frequently complained of dyspnea than men (27 p.c. vs 12 p.c.; p = 0.018). Conclusion: Our study suggests that among ambulance transported patients with suspicion of acute coronary syndrome there are some differences between women and men in terms of their characteristics and underlying etiology, but not in terms of the risk of mortality.

184/2523

Tytuł oryginału: A multi-center open study to determine the effect of lamivudine on HBV DNA clearance and to assess the safety of the regimen in patietns with chronic hepatitis B infection.
Autorzy: Mazur Włodzimierz, Król Franciszek, Cianciara Janusz, Nazzal Khalil, Gładysz Andrzej, Juszczyk Jacek, Bolewska Beata, Adamek Jacek, Czajka Barbara, Świętek Katarzyna, Kryczka Wiesław, Gonciarz Zbigniew
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR257-CR262, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Patietns with on-going HBV viral replication often present with clinical features of active chronic hepatitis. Until the recent introduction of nucleoside analogues, interferon-alpha was the only approved drug for these patietns. One of the former drugs, lamivudine, has been shown in clinical trials in the US and Asia to effectively inhibit the viral polymerase of HBV. Our study was undertaken to assess the efficacy and safety of lamivudine therapy in Polish patietns with chronic hepatitis B. Material/Methods: Forty-five patients with chronic hepatitis B (HBeAg positive, anti-e negative, HBV-DNA positive by hybridization assay) were enrolled in the study. The patietns received 100 mg of lamivudine orally, once daily for 12 months. They returned for routine clinical and laboratory control every two weeks during the first months of treatment, and later at 3-month intervals whle receiving lamivudine. Results: At the end of treatment, serum HBeAg was not detected in 21 patients (48.8 p.c.), and anti-HBe appeared in the serum of 19 patients. 37.2 p.c. of the patietns in the study group showed sustained suppression of serum HBV DNA at the end of treatment. Lamivudine therapy was well tolerated, with the rate of occurrence of adverse events similar to that observed in other clincial studies. Conclusions: 12-month lamivudine therapy in this Polish population of patients with chronic hepatitis B induced a high rate of HBeAg seroconversion, accompaneid by reduction of HBV-DNA and the normalization of alanine aminotransferase activities.

185/2523

Tytuł oryginału: Level of antithrombin III, protein C, protein S and other selected parameters of coagulation and fibrinolysis in the blood of the patietns with recurrent deep venous thrombosis.
Autorzy: Świątkiewicz Andrzej, Jurkowski Piotr, Kotschy Maria, Ciecierski Marek, Jawień Arkadiusz
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR263-CR268, il., tab., bibliogr. 49 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Thrombophilia is caused mainly by disturbances of hemostasis involving excessive coagulation system activation, reduction of anticoagulation system (antithrombin III, protien C, protien S, RAPC) or fibrinolytic activity. Material/Methods: In 34 young patietns (aged 40 years) with recurrent deep venous thrombosis ( 2 incidents) the activity of antithrombin III, protien C, S, platelet count, adhesion and aggregation, APTT, stipven-kephalin, prothrombin time and INR were investigated. Fibrinogen, factor XIII, ELT, FDP, Ag t-PA levels, antigen concentration and PAI-1 activity were determined. Patietns with idiopathic DVT, after elimination of most important thromboembolism risk factors, were qualified for the study. DVT was confirmed in all patients by phlebography, plethysmography and ultrasonography. Results were compared with a group of 54 healthy controls. Results: In almost 50 p.c. of patients with recurrent DVT (15/34) decrease of at least one plasma coagluation inhibitor (AT III, PC, PS) level was observed. In the patient group (with AT III and/or PC and/or PS decrease) statistically significant reduction of kaolin-kephalin time in comparison with controls was observed (ŕ 0.01). Analysis of fibrinolysis system demosntrated significant factor XIII level decrease (to 58.3 p.c.), fibrinogen level increase, ELT prolongation, and fibrinogen and fibrin degradation product increase in comparison with controls. THe patients demonstrated 3-fold higher t-PA antigen level (13.1 ng/ml, ŕ 0.0001) and over 3-fold higher PAI-1 activity (26.7 AU/ml, ŕ 0.001) than healthy controls...

186/2523

Tytuł oryginału: Assessment of iron metabolism in children with chronic hepatitis B - prognostic factor in interferon alpha therapy.
Autorzy: Chrobot Andrzej, Chrobot Agnieszka Monika, Szaflarska-Szczepanik Anna
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR269-CR273, tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The aim of the study was to assess iron metabolism in children with chronic hepatitis B and to establish whether it had any influence on the results of interferon alpha therapy. Material/Methods: The study was carried out in a group of 38 children aged from 2 to 16 years with chronic hepatitis B diagnosed according to serological, biochemical and histopathological criteria. All the patietns were treated according to the commonly adopted schedule: interferon alpha administered subcutaneously three times a week at 3 MIU doses for 20 weeks. During the therapy and one-year follow-up after its completion, biochemical liver function parameters, serological HBV markers, and iron and ferritin levels were monitored. Results: The therapy resulted in obtaining seroconversion in the HBe system in 9 patients (23.68 p.c.). Liver bioptates in that group of patients demonstrated more advanced changes due to inflammatory activity and fibrosis processes, significantly higher values of alanine aminotransferase, lower serum levels of iron and ferritin with more pronounced difference in ferritin levels, although the differences reached no statistical significance. Conclusions: No disturbances of iron metabolism were observed in children with chronic hepatitis B. In the group of patietns with detectable seroconversion in the Hbe system resulting from interferon alpha therapy, lower serum levels of iron and ferritin were observed. Reoutine determinations of serum iron and ferritin levels as a prognostic factor for positive response to interferon alpha seem to be of little use, especially in children, in whom no iron accumulation in liver tissue is observed in histopathological assessment of liver bioptates.

187/2523

Tytuł oryginału: Course of viral hepatitis B and combined B and C hepatitis in children treated for neoplastic diseases.
Autorzy: Kołtan Sylwia, Wysocki Mariusz, Kołtan Andrzej, Świątkiewicz Violetta, Styczyński Jan, Dębski Robert, Balcar-Boroń Anna
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR274-CR279, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Viral hepatitis C in children is milder than in adults. Patients with tumors, because of immune deficits, seem to be at risk of rapid liver disease progression. Little is known about the course of HCV and dual HBV-HCV infections in such patients. Material/Methods: The study assessed clinical courses of HCV and dual HBV-HCV infections in 249 children with tumors aged 1-18 years, divided into four groups: with HCV infection - 53, HBV-HCV infection - 53, HBV only - 33 and ifection-free - 110. Results: Hepatitis C was often chronic - in 83.3 p.c of patients with HCV and 77.3 p.c. of those with dual infection. Over 80 p.c. had no jaundice. Mean AIAT activity was the highest in the HCV group (149.2 ń 202.7 IU/l), lower in the dual infection group (123.3 ń 148.1 IU/l; p 0.02), and the lowest in HBV (92.7 152.9 IU/l. Transaminasemia course in patients with HCV and dual HBV-HCV infections was most frequently fluctuating (50.9 p.c. and 45.3 p.c., respectively). 52.8 p.c. of children with HCV demonstrated no advanced inflammatory lesions with invasion of the lamina limitans, no marked fibrosis, and minimum changes in descriptive classification. In 92.3 p.c. of children with dual infections, inflammatory activity was mild or mean, with no fibrosis in 61.5 p.c., and persistnet chronic inflammation predominant in descriptive classification (46.2 p.c.). Conclusions: HCV an HBV-HCV infections in children with neoplastic diseases tend to take chronic forms, but their clinical course is mild with signs of severeinflammatory activity or hepatitic strom aremodeling observed on histopathology.

188/2523

Tytuł oryginału: Total IgE levels and skin test results in children under three years of age with food hypersensitivity.
Autorzy: Malinowska Elżbieta, Kaczmarski Maciej, Wasilewska Jolanta
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR280-CR287, il., tab., bibliogr. 43 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The most common tools of allergologic diagnostics are skin prick tests (SPT) and total serum IgE (IgE total) determinations. Material/Methods: The study was carried out in a group of 348 children aged 5 to 36 months. All the children underwent SPT using 12 food allergens and in 229 of them also 9 inhalant allergens were tested. Assessment of test positivity utilized relative criteria, i.e. the result was regarded as positive if a wheal with S surface area exceeding or equal to 0.25 of the surface area SO of a control wheal was formed. In this study group, 291 had IgE total levels determined. They were divided into the groups with elevated and normal IgE total levels on the basis of two standards, i.e. the standards recommended by the manufacturer of the test reagents used to determine IgE total (BioWhittaker) and the population standards. Resutls: Positive SPT results for food allergens were obtained in 15.8 p.c. of the examined children, for inhalant ones in 21.8 p.c. The factors taken into account in the analysis of impact on SPT results included age, sex, living place and the use diet. Elevated IgE total levels according to the manufacturer's standards were detected in 35.0 p.c., and according to the population standards in 67.0 p.c. of the examined children. The obtained positive test results were compared with elevated IgE total in the studied patients.Conclusions: 1. Feasibility of SPT at this age. 2. Determination of normal IgE total range in the group of children below three requires further studies.

189/2523

Tytuł oryginału: Salt iodination as a effective method of iodine supplementation.
Autorzy: Grzesiuk Wiesław, Kondracka Agnieszka, Słoń Milena, Wojda Marzena, Nauman Janusz
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR288-CR291, il., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: In January 1997 a new approach to preventing iodine deficiency was introduced in Poland. The goal of the present study was to determine whether the mandatory iodization of kitchen salt (30 mg KI/kg) has had any impact on ioduria. Material/Methods: The study was performed on 29 healthy volunteers, aged 22-29 (average age 23.93 ń 1.14), examined in 1996 and retested after 36 months of iodine supplementation in 1999. Each person underwent a physical examination and thyroid ultrasonography. The iodine level was determined using morning urine samples. Results: For the duration of the study thyroid volume remained unchanged, ranging from 11.48 ń 3.89 ml in 1996 to 12.11 ń 3.85 ml in 1998. THe echostructure was normal in all subjects both in 1996 and in 1999. In 1996 the iodine concentration in urine (ICU) in the study group averaged 100.4 ń 41.5 ćg/L (range from 43.3 to 175.6 ćg/L). Seventeen persons had an iodine cncentration below 100 ćg/L. In 1999 the average ICU was 140.7 ń 78.87 ćg/L. Three subjects had a lower ICU in 1999 (53.93 ń 24.65 ćg/L) than in 1996 (104.1 ń 24.6 ćg/L). These persons claimed to have completely eliminated the use of added salt in their diet. Conclusion: We conclude that mandatory iodine supplementation method is sufficiently effective when salt is used in the diet. In the case of restrictions on salt consumption the use of preparations containing iodine should be recommended.

190/2523

Tytuł oryginału: Drug-induced liver damage - a three-year study of patients from one gastroenterological department.
Autorzy: Hartleb Marek, Biernat Łukasz, Kochel Anna
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR292-CR296, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bakcground: The aim of our study was to analyze drug-induced liver disease over a 3-year period in one gastroenterological department. Material/Methods: International consensus standard definitions and criteria for assessing causality of adverse drug reactions were applied to all patients with abnormal hepatic test results. Results: Drugs were implicated in hepatic injury in 14 patietns (8 females) in whom causal relationship between drug and liver disease was definite or highly probable. The drugs responsible were amoxicillin with clavulanic acid (3 cases), fluvastatin and pravastatin (3 cases), antituberculous drugs (2 cases), estrogens, roxithromycin, asacol, satolol, enalapril and thiamazol. A total of 78.6 p.c. (11 cases) were classified as hepatocellular or mixed hepatitis, while cholestatic injury was found in 21.4 p.c. (3 cases). There were no lethal or severe (prothrombin 50 p.c.) hepatic drug reactions. In 13 patietns the course of liver disease after with drawal of the offensive drug was either acute or protracted, while in one patient there was chronic cholestasis ( 3 years) resulting from injury to interlobular bile ducts by amoxicllin with clavulanic acid. Conclusion: A thorough history of drug intake should be taken in all patients presenting with abnormal hepatic test results. Amoxicillin & clavulanic acid, cholesterol-lowering and antituberculin drugs were the most frequent hepatotoxic factors in our patients. In a majority of cases the liver injury was not severe, and resolved after prompt withdrawal of the responsible drug.

191/2523

Tytuł oryginału: Efficacy and safety of doxofylline compared to theophylline in chronic reversible asthma - a double-blind randomized placebo-controlled multicentre clinical trial.
Autorzy: Goldstein Marc F., Chervinsky Paul
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR297-CR304, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Experimental studies have shown that doxofylline is endowed with a remarkable bronchodilator activity with less extra-respiratory effects than theophylline. This trail was designed to compare the efficacy and safety of doxofylline, theophylline, and placebo in patients with chronic reversible bronchial asthma. Material/Methods: Three hundred forty-six patietns were randomly assigned to a 12-week oral treatment with either doxofylline 400 mg t.i.d. (high dose), doxofylline 200 mg t.i.d. (low dose), theophylline 250 mg t.i.d. (active control) or placebo. Pulmonary function tests (PFTs) were performed biweekly. Patients kept records of peak flow meter (PFM) measurements, asthma attack rate and beta-2-agonist use (albuterol). Results: Changes in FEV1 2 hours after the administration of treatments versus baseline exhibited statistically significant differences between doxofylline 400 mg t.i.d. and placebo and between theophylline and placebo. Similar differences were monitored on the other variables (FVC, PFER, FEF25-75 p.c. Asthma attack rate and use of albuterol decreased remarkably with doxofylline 400 mg t.i.d. and theophylline. There were few statistically significant differences between doxofylline 200 mg t.i.d. and placebo. Significantly more patients had to interrupt treatment because of adverse events under theophylline than under doxofylline 400 mg t.i.d. (p=0.001). With doxofylline 400 mg t.i.d., the number of patietns treated to spare one dropout due to theophylline was 5. Conslusion: This study provides evidence that doxofylline 400 mg t.i.d. is an effective treatment for relieving airway obstruction and displays a better safety profile with respect to theophylline 250 mg t.i.d. with a favorable risk-to-benefit ratio.

192/2523

Tytuł oryginału: Comorbid disease profiles of adults with end-stage hip osteoarthritis.
Autorzy: Marks Ray, Allegrante John P.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CR305-CR309, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: Although hip osteoarthritis is a common disabling condition, an incomplete understanding of all factors that contribute to hip osteoarthritis disability persists. The objective of the study was to improve our understanding of potentially preventable factors that might influence hip osteoarthritis disability by examining the prevalence and impact of selected comorbid factors potentially associated with the progression and severity of hip joint osteoarthritis among patietns with end-stage hip osteoarthritis. Material/Methods: The mdical records of 1,000 hip osteoarthritis surgical patients were assessed to specifically identify the presence or absence of comorbid conditions of the cardiac, vascular or respiratory systems. The assocaition between the patient's functional status before and after surgery, and the presence or absence of these comorbid conditions, was assessed. Results: Over 55 p.c. of the sample had at least one comorbid condition, such as hypertension or cardiac disease. After controlling for age, trauma, type of surgery and gender discrepancies, those with two or more comorbidities were found to have greater degrees of functional impairment before surgery, and lower post-operative functional status after surgery than those with no coexistent disease (P 0.05). Conclusion: Patietns with end-stage hip osteoarthritis exhibit a high prevalence of vascualr-related comorbidities. THe disability of patients with hip osteoarthritis is increased in the presence of two or more related comorbid conditions. These findings suggest a novel focus for primary, secondary and tertiary preventive strategies.

193/2523

Tytuł oryginału: Combination of pamidronate and thalidomide in the therapy of treatment-resistant multiple myeloma.
Autorzy: Ciepłuch Hanna, Baran Wojciech, Hellmann Andrzej
Źródło: Med. Sci. Monitor 2002: 8 (4) s.PI31-PI36, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Bisphosphonates inhibit the activity of osteoclasts and demonstrate antitumor effect, involving induction of plasmocyte apoptosis, blocking of angiogenesis, stimulation of Tçë lymphocytes and inhibition of metalloproteinases. Bisphosphonates combined with thalidomide, a drug possessing confirmed anti-myeloma activity, seem to have potential favorable effect in patietns with treatment-resistant multiple myeloma with advanced osteolytic lesions. Material/Methods: 13 patietns with treatment-resistant multiple myeloma with advanced osteolytic lesions received combined pamidronate and thalidomide therapy. All the patietns underwent detailed clinical and laboratory control once a month. Pamidronate was used at 90 mg iv dose administered at 4-week intervals, and thalidomide at doses escalated from 200 mg/d in the first week to 400 mg/d after 3 weeks of the therapy. The mean duration of treatment was 12 weeks with a range of 3 to 36 weeks. Results: 7 patietns (53 p.c.) receiving pamidronate and thalidomide therapy demonstrated good response involving at lesast 25 pc. reduction of monoclonal protein levels in comparison with baseline. All these patietns reported improvement of osteodynia symptoms, and 3 of them - thier complete regression. 70 p.c. patients experienced side effects (dizziness, constipation, somnolence, polyneuropathy) due to thalidomide administration. Conclusion: Good response to combined pamidroante and thalidomide therapy can be expected in at least 50 p.c. of patietns with treatment-resistant multiple myeloma with advanced osteolytic lesions.

194/2523

Tytuł oryginału: Effect of diazepam and clonazepam on the function of isolated rat platelet and neutrophil.
Autorzy: Rajtar Grażyna, Żółkowska Dorota, Kleinrok Zdzisław
Źródło: Med. Sci. Monitor 2002: 8 (4) s.PI37-PI44, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: Benzodiazepine binding sites distnict from the GABA-receptor-chloride-complex in the central nervous system have been recongized in many peripheral tissues, but their physiological role remains unexplained. Our study was undertaken to examine the effects of diazepam, clonazepam, and PK 11195, a peripheral benzodiazepine receptor antagonist, on the functional and biochemical responses of platelets and neutrophils stimulated by different physiological agonists. Material/Methods: The experiments were conducted on isolated washed rat platelets activated by arachidonic acid (AA), adenosine 5'-diphosphate (ADP), or thrombin and on isolated rat neutrophils activated by a chemotactic peptide, formyl methionyl leucyl phenylalanine (fMLP). Results: The results showed that neither diazepam nor clonazepam nor PK 11195 alone augmented the reponse of resting platelets or modified neutrophil reponse, but diazepam and clonazepam in a concentration-dependent manner inhibited thrombin, ADP or AA-stimulated platelet aggregation and the thrombin-induced increase in free intracellular Caý+. Both drugs also exerted an inhibitory effect on reactive oxygen species (ROS) produced by fMLP-stimulated neutrophils. However, diazepam was about 10 times more effective than clonazepam. PK11195 did not influence platelet and neutrophil function stimulated by agonists, but reversed the inhibitory action of both benzodiazepines on platelet activation and ROS production. Conclusions: The results indicated that invitro diazepam, and in a much smaller degree clonazepam, may down-regulate platelet activation and release of some proinflammatory mediators by stimulated neutrophils. These effects are probably exerted by a specific benzodiazepine binding sites.

195/2523

Tytuł oryginału: Rehabilitation outcome in a patient awakened from prolonged coma.
Autorzy: Talar Jan
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CS31-CS38, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: This article describes the rehabilitation of a patient recovering from a prolonged coma (defined as lasting longer than 4 weeks). The case is noteworthy because it exemplifies the possibilities and difficulties entailed in treating these patients, who are often regarded as too severely impaired to justify intensive rehabilitation efforts. Case report: The patietn is a 28-year old Polish male, unmarried, who suffered serious closed head injuries in an automobile accident in April of 1999. He was in a comatose state for more than two months, with a GCS score of 5. When admitted for rehabilitation he was bedriedden, wht global aphasia, agraphia, limb apraxia, and executive dysfunction. THe rehabilitation program developed for him is described in detail. Results: Over the course of rehabilitation, which began in December 1999 and continues to this writing, the patient has regained locomotion capabilities (though with impairments), and his speech has improved considerably. The apraxia has largely resolved, and he is able to write his name and copy words. he is now capable of performing many activities of daily living. Conclusions: A comprehensive program of rehabilitation characterized by a strategic, heurisitic approach is capable of achieving a good outcome even in very difficult cases, such as prolonged coma.

196/2523

Tytuł oryginału: Non-valvular isolated pulmonary artery vegetations.
Autorzy: Farsak Bora, Yilmaz Mustafa, c Mehmet, zkutlu Sheyla, Demircin Metin
Źródło: Med. Sci. Monitor 2002: 8 (4) s.CS39-CS41, il., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

niemowlęta

płeć męska

Streszczenie angielskie: Background: One of the serious potential complications of complex congenital heart defect (CHD) is bacterial endocarditis. Right sided endocarditis accounts for 5-10 p.c. of all cases of infective endocarditis, with the tricuspid valve more frequentlly affected than the pulmonary valve. Pulmonary artery involvement in bacterial endocarditis is very rare, but when it exists it is almost always associated with endocarditis of the pulmonary valve: isolated pulmonary artery vegetations have not previously been reported before. Case Report: A 6-year-old boy with atrial septal defect (ASD), ventricular septal defect (VSD), coarctation of the aorta (CA), persistent ductus arteriosus (PDA) and pulmonary hypertension, who had been operated for closure of PDA, correction of CA and pulmonary artery banding, was readmitted with bacterial endocarditis. In surgery, the VSD was closed with a Dacron patch using interrupted pledget buttressed sutures, and the ASD was closed using primary sutures. After debanding, while pulmonary arteriotomy was performed for patch angioplasty, we observed that the entire pulmonary artery trunk, right and left pulmnary artery branches, were filled with a solid mass, which was successfully treated by surgical excision. Conclusion: With ongoing improvements in diagnostic tools, especially in echocardiography, antibiotic therapy and surgery, CHD can now be treated successfully in early infancy or childhood.

197/2523

Tytuł oryginału: Dynamic magnetic resonance imaging in soft tissue tumors - assessment of the diagnostic value of tumor enhancement rate indices.
Autorzy: Tacikowska Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (4) s.MT53-MT57, tab., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Background: Dynamic MRI is routinely used in the diagnosis of soft tissue tumors to supplement static imaging. The aim of our study was to assess the diagnostic value of dynamic MRI using the coefficient of total tumor enhancement expressed as per cent (tec p.c.) and the tumor enhancement rate coefficient expressed as per cent per minute (erc p.c./min), and to make a comparative analysis of the sensitivity and specificity of both parameters. Material/Methods: The material consisted of 45 patietns (30 sarcomas, 15 non-malignant lesions). The age of the pateitns was 16-64 years. The MRI was done using an Elscint 2T unit, applying gradient echo techniques. The coefficient of total contrast enhancement (tec p.c.) and the tissue enhancement rate (erc p.c./min) were calcuatated. The limit values were determined for dividing the tumors into sarcomas and non-malignant lesions. The sensitivity and specificity of MRI were calculated and compared using both indices. Results: Dynamic MRI with calculation of erc p.c./min and tec p.c. makes it possible to differentiate between sarcomas and non-malignant lesions. Conclusions: The erc p.c./min was a more sensitivie than tec p.c. differentiating parameter in the diagnosis of soft tissue tumors.

198/2523

Tytuł oryginału: A radical approach to cancer.
Autorzy: Das Undurti N.
Źródło: Med. Sci. Monitor 2002: 8 (4) s.RA79-RA92, il., bibliogr. 173 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Reactive oxygen species are known to be potentially dangerous, but are also needed for signal-transduction pathways. Tumor cells have relatively low amounts of superoxide dismutase (SOD), which quenches superoxide anion (o2) and as a result of a higher level of aerobic metabolism, higher concentrations of O2, compared to normal cells. But this may not be true of all tumor cells. Some tumor cells have relatively higher amounts of vitamin E, a potent antioxidant, and a higher level of anaerobic metabolism, resulting in a balance that is tilted more towards higher anti-oxidant capacity. In both instances of higher aerobic and anaerobic metabolism methods designed to augment free radical generation in tumor cells can cause their death. It is suggested that free radicals and lipid peroxides suppress the expression of Bcl-2, activate caspases and shorten telomere, and thus inducing apoptosis of tumor cells. Ionizing radiation, anthracyclines, bleomycin and cytokines produce free radicals and thus are useful as anti-cancer agents. But they also produce many side-effects. 2-methyoxyoestradiol and polyunsaturated fatty acids (PUFAs) inhibit SODs and cause an increase of O2 in tumor cells leading to their death. In addition, PUFAs (especially gamma-linolenic acid), 2-methoxyoestradiol and thalidomide may possess anti-angiogenic activity. This suggests that free radiocals can suppress angiogenesis. Limited clinical studies done with gamma-linolenic acid showed that it can regress human brain gliomas without any signficant side-effects. Thus, PUFAs, thalidomide and 2-methoxyoestradiol or their derivatives may offer a new radical approach to the treatment of cancer.

199/2523

Tytuł oryginału: Effects of acamprosate and some polyamine site ligands of NMDA receptor on short-term memory in rats.
Autorzy: Mikołajczak Przemysław, Okulicz-Kozaryn Irena, Kamińska Ewa, Niedopad Lidia, Polańska Anna, Gębka Joanna
Źródło: Eur. J. Pharmacol. 2002: 444 (1/2) s.83-96, il., tab., bibliogr. s. 94-96
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

szczury

Streszczenie angielskie: The aim of this study was to evaluate the effect of multiple acamprosate (500.0 mg/kg, p.o.) administration on short-term memory, using te social recognition test in rats. Ifenprodil (1.0 mg/kg, i.p.), arcaine (5.0 mg/kg, i.p.) and spermidine (20.0 mg/kg, i.p.) were chosen as polyamine ligands and their action or interaction with acamprosate was also studied. The doses used did not show any sedative activity, which was assessed by measuring locomotor activity and the hypnotic effect of ethanol. The findings suggest that acute acamprosate treatment did not impair short-term memory. Multiple acamprosate and a single spermidine or arcaine administration led to better performance in the memory test, whereas no significant difference was observed in ifenprodil-treated rats. Co-administratio of a single arcaine or spermidine dose with multiple acamprosate produced worse results. This means that the effect of repeated acamprosate administration can be changed by the co-administration of other polyamine ligands, so that care should be taken in interpreting.

200/2523

Tytuł oryginału: Repeated imipramine and electroconvulsive shock increase ŕ1A-adrenoceptor mRNA level in rat prefrontal cortex.
Autorzy: Nalepa Irena, Kreiner Grzegorz, Kowalska Marta, Sanak Marek, Zelek-Molik Agnieszka, Vetulani Jerzy
Źródło: Eur. J. Pharmacol. 2002: 444 (3) s.151-159, il., tab., bibliogr. s. 158-159
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

szczury

Streszczenie angielskie: ŕ1-Adrenoceptors have been implicated in the mechanism of action of antidepressants, but their action on specific receptor subtypes was rarely reported. We compared now the action of two prototypic antidepressant treatments: repeated imipramine and electroconvulsive shock, on the expression of the ŕ1A - and ŕ1B -adrenoceptor mRNAs and on the receptor density in rats. The mRNA expression was assessed by Northern blot in the prefrontal cortex and the hippocampus, the receptor density was measured by [3H] prazosin binding in the total cerbral cortex and hippocampus. In the cortex, both treatments elevated the ŕ1A -adrenoceptor mRNA and the expression of receptor protein. The expression of ŕ1B -adrenoceptor mRNA remained unaffected. In contrast, in the hippocampus, the antidepressant treatments augmented the density of ŕ1A -adrenoceptor protein without changing the level of its mRNA expression there. The results suggest that the ŕ1A -adrenoceptor subtype is specifically involved in the mechanism of action of classical antidepressant treatments.

201/2523

Tytuł oryginału: Antigen levels of the urokinase-type plasminogen activator and its gene polymorphisms in colorectal cancer.
Autorzy: Przybyłowska K., Smolarczyk K., Kulig A., Romanowicz-Makowska H., Dziki A., Ulańska J., Pander B., Błasiak J.
Źródło: Cancer Lett. 2002: 181 (1) s.23-30, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 306,023

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We analysed the distribution of genotypes of two polymorphisms in the urokinas-type plasminogen activator (uPA) gene: C - T substitution in exon 6 and T - C substitution in intron 7 in 52 subjects with colorectal cancer. Genotypes were determined in tumour tissue and distant mucosa samples by allele-specific polymerase chain reaction. The antigen levels of uPA in cancer tissue were higher than in distant mucosa as measrued by enzyme-linked immunosorbent assay. The level of uPA antigens in cancer samples with the C/C genotype of C - T polymorphism in exon 6 was higher than in samples with C/T and T/T genotypes. No differences in the level of uPA antigens between the alleles of the intron 7 T - C polymorphism were found. As uPA can be involved in cancer invasion and metastasis, C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression.

202/2523

Tytuł oryginału: Hemodynamic performance after stented vs stentless aortic valve replacement.
Autorzy: Jasiński M. J., Hayton J., Kadziola Z., Woś S[tanisław], Sosnowski A. W.
Źródło: J. Cardiovasc. Surg. 2002: 43 (3) s.313-317, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 311,845

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background. Stentless bioprostheses are anticipated to cause improved hemodyanmics and increased longevity over stented bioprosthesis. We have compared echocardiographic analysis of stented bioprosthesis "Freestyle" with stented "Mosaic" bioprosthesis. Because of similar technology (0 pressure fixation, anticalcification) any differences may relate to stent. Methods. Twenty-eight patients undergoing AVR were randomly assigned to receive either stented or stentless. Echocardiograms, by means of M-mode and Doppler were performed early, 3-6 months and 1 year postoperatively. Results. The peak flow velocity was significantly lower in teh stentless group, especially 1 week and 6 months after surgery. Mean transvalvular gradient dropped significantly in stentless group and did not change in stented group. EOA did not change significantly in either of groups. AoV velocity time integral was increasing in stentless group. LV mass had fallen significantly in both groups but degree of mass reduction was comparable. Conclusions. There are marked improvements of stentless valves hemodynamics. However it is not necessary equal to higher degree of LV mass reduction during 1 year follow-up.

203/2523

Tytuł oryginału: Substance P content in the cerebrospinal fluid and fluid perfusing cerebral ventricles during elicitation and inhibition of trigemino-hypoglossal reflex in rats.
Autorzy: Zubrzycka Maria, Janecka Anna
Źródło: Brain Res. 2002: 941 (1/2) s.29-33, il., bibliogr. 47 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The aim of this study was to establish whether tooth pulp and periaqueductal central gray (PAG) stimulation affects the release of substance P (SP) into the fluid perfusing the cerebral ventricles in rats. The content of substance P in the cerebrospinal fluid and fluid perfusing cerebral ventricles was determined during incisor pulp stimulation with electrical impulses inducing nociceptive trigeminohypoglossal reflex and then during inhibition of the reflex by stimulation of PAG. Perfusion of the cerebral ventricles was carried out using artificial cerebrospinal fluid (aCSF). SP-like immunoreactivity (SP-LI) was determined in the samples by radioimmunoassay. Samples were collected in four groups: first group - cerebrospinal fluid (CSF); second group - aCSF perfusates without stimulation; third group-aCSF perfusates during incisor pulp stimulation; fourth group-aCSF perfusates during incisor pulp stimulation and simultaneous inhibition of trigemino-hpoglossal reflex by PAG stimulation. It was shown that incisor pulp stimulation led to the increased release of SP-LI into the fluid perfusing cerebral ventricles. Stimulation of PAG reduced the release of SP-LI into the cerebro-ventricular system to the values obtained before the tooth pulp stimulation. The results indicate that PAG significantly inhibits the release of SP-LI into the rat cerebral ventricular system and may be involved in the inhibition of trigemino-hypoglossal reflex.

204/2523

Tytuł oryginału: The beginnings of urology in Kraków: on the 70th anniversary of the founding of the Clinic of Urology.
Autorzy: Dobrowolski Z. F., Swolkien J., Dobrowolska B., Lipczyński W., Jaszczyński J.
Źródło: Br. J. Urol. 2002: 89 (6) s.596-598, bibliogr. 4 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

205/2523

Tytuł oryginału: Survival in patients with rare subtypes of renal cell carcinoma.
Autorzy: Grabowski M[arek], Huzarski T., Lubiński J., Sikorski A[ndrzej]
Źródło: Br. J. Urol. 2002: 89 (6) s.599-600, tab., bibliogr. 14 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective. To evaluate the survival of patietns with rare malignant histological subtypes of renal cancer. Pateitns and methods. The Heidelberg classification of renal cell carcinoma (RCC) divides tumours into clear cell carcinoma (CCC), papillary cancer (PC), chromophobic cancer (ChC) and collecting duct carcinoma (CDC). Sarcomatoid tumours are in a different subgroup treated as a final stage of histological progression. Between 1990 and 1997, 319 nephrectomies were undertaken because of RCC in 317 patients. In 42 patietns (13 p.c.) the patholgoical findings showed other than CCC; in 13 PC was confirmed histolgoically, in nine ChC, in 11 a mixed type of CCC and sarcomatoid type, in seven a sarcomatoid tumour and in four, CDC. Results. One patietn of the 13 with PC and two of the nine with ChC died. The worst prognosis was in those with CDC, CCC-sarcomatoid and sarcomatoid tumours, as all these patietns died. Conclusion. The histopathological differentiation of RCC into subtypes gives additional useful prognostic information.

206/2523

Tytuł oryginału: Assessing the vascular-stromal coefficient in patients with benign prostatic hyperplasia or prostate cancer using transrectal ultrasonography and power Doppler analysis.
Autorzy: Dobrowolski Z., Jaszczyński J., Drewniak T., Habrat W.
Źródło: Br. J. Urol. 2002: 89 (6) s.601-603, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Objectives. To assess a coefficient of theprotatic blood supply in patietns with prostate cancer or benign prostatic hyperplasia (BPH), and thus improve the diagnostic and prognostic variables needed to evaluate patients with prostate cancer. Patietns and methods. The vascular-stromal coefficient was defined as the ratio of the blood vessel surface area and the surface area of the prostate, using power Doppler functions and transrectal ultrasonography. The index was calcualted for the plane of the prostatic surface from the apex to the widest points of the peripheral zone, and for the plane across the highest density of blood vessels in the transition zone. A micobubble contrast agent was used to intensify the acoustic signals from prostatic vessels. The images were reconstructed in three dimensions. In all, 61 patietns with BPH and 32 with confirmed prostate cancer were examined, and compared with a control group of young men. Results. In the horizontal plane crossing the apex of the prostate, the vascular-stromal coefficient was 0.0054 for the controls, 0.0072 for BPH and 0.0067 for cancer. At the level of the highest density of blood vessels the respective values were 0.0083, 0.038 and 0.029. The respective values for the first plane after using the contrast agent were 0.025, 0.092 and 0.0365, and in the second level 0.04, 0.15 and 0.14. Conclusion. The vascular-stromal coefficient measured at both levels was higher in patietns with BPH than in those with cancer or than in the controls. The prostatic vascular system can be computer-reconstructed in three dimensions, allowing an evaluation of asymmetry and other vascular abnormalities...

207/2523

Tytuł oryginału: Correlations among prostatic-specific antigen, Gleason score, staging and grading in patients after radical prostatectomy.
Autorzy: Szymanowski J., Dudek P., Ratajski J., Wysocki M.
Źródło: Br. J. Urol. 2002: 89 (6) s.612-613, tab., bibliogr. 6 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Objectives. To evaluate the correlation between Gleason grade, T stage, tumour grade and preoperative prostatespecific antigen (PSA) level ( or 20 ng/mL) in patietns after radical prostatectomy (RP). Patietns and methods. The serum total PSA level was measured 3 months before surgery in 87 patients who then undrewent RP between 1994 and 2000; the tumour specimens were staged and graded. Results. There were 57 (66 p.c.) patietns with a PSA level of 20 ng/mL; in this group only four (7 p.c.) patients had a Gleason grade of 7 and eight (14 p.c.) had G3 tumours. Thirty (34 p.c.) patients had a PSA level of 20 ng/mL; in this group only two (7 p.c.) patietns had a Gleason grade of 7 and 26 (87 p.c.) had G1 and G2 tumours. Conclusions. These results confirm our previous observations that the PSA level cannot be use as the only factor to indicate RP in patients with prostate cancer, but further evaluation on more patietns is needed.

208/2523

Tytuł oryginału: Vascular angiographic asymmetry on three-dimensional transrectal power Doppler ultrasonography in patients with organ-confined prostate cancer.
Autorzy: Dobrowolski Z. F., Jaszczyński J., Drewniak T., Habrat W., Kusionowicz J.
Źródło: Br. J. Urol. 2002: 89 (6) s.614-615, bibliogr. 7 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Objective. To assess whether organ-confined prostate cancer not detected on routine transrectal ultrasonography (TRUS) can be detected using threedimensional (3-D) power Doppler methods. Patients and methods. A preliminary trial of the use of TRUS with 3-D power Doppler image reconstruction was conducted in 116 patients being evaluated for lower urinary tract symptoms. Using a microbubble enhancing agent, a adequate horizontal plane allowed the simultaneous visualization and assessment of the symmetry of echogenicity and vascularization of the peripheral area of both prostate lobes, which is not possible in standard TRUS. The peripheral zone vascular asymmetry in the horizontal plane was also assessed retrospectively n those patients with pT2a,b prostate carcinoma. Results. Stage pT2a,b prostate carcinoma was confirmed in 26 patients; in this group, two independent investigators confirmed the vascular asymmetry between the left and right peripheral zone, in the horizontal plane, in 22 patients, giving a sensitivity of 85 p.c. Conclusions. Vascular asymmetry allows guided biopsy in lesions that are invisible using standard TRUS. Microbubble agents are an effective method of increasing the acoustic signals from small vessels of the prostate.

209/2523

Tytuł oryginału: Evaluation of the influence of systemic neoadjuvant chemotherapy on the survival of patietns treated for invasive bladder cancer.
Autorzy: Kolaczyk W., Dembowski J[anusz], Lorenz J[erzy], Dudek K.
Źródło: Br. J. Urol. 2002: 89 (6) s.616-619, il., bibliogr. 13 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Objective. To assess the influence of neoadjuvant systemic chemotherapy using a modified methotrexate, vinblastine, doxorubicin and cisplatin (MVAC) scheme in patietns with invasive bladder cancer. Patients and methods. Two groups of patients were reviewed retrospectively: group 1 included 51 who received chemotherapy before cystectomy and group 2 included 62 who were treated only with surgery. The mean (range) duration of follow-up was 3.2 (0.25 - 10.25) years. The patients in group 1 were divided into two subgroups: those with tumour confined to the bladder (T1, T2 and T3a) and the remaining patients with tumour beyond the bladder (T3b, T4a,b). The chemotherapy was administered as routine MVAC, except vinblastine and methotrexate were given at 15 and 22 days during the cycle. A mean of three cycles were administered. Results. The 5-year survival rate in group 1 and 2 was 66 p.c. and 58 p.c., respectively (P 0.3); after 8 years of follow-up the survival rates were 58 p.c. and 33 p.c., respectively, and significantly different (P 0.01). Conclusion. Systemic chemotherapy using the modified MVAC scheme in patients subsequently undergoing radical cystectomy improved the survival rate after 8 years of follow-up.

210/2523

Tytuł oryginału: Treatment of high-risk superficial bladder cancer with maintenance bacille Calmette-Gurin therapy: preliminary results.
Autorzy: Kołodziej A., Dembowski J[anusz], Zdrojowy R[omuald], Woźniak P., Lorenz J[erzy]
Źródło: Br. J. Urol. 2002: 89 (6) s.620-622, tab., bibliogr. 6 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective. To evaluate, in a prospective study, the effects and results of maintenance therapy with bacille Calmette-Gurin (BCG) in treating patietns with high-risk superficial bladder cancer. Patients and methods. In all, 155 patietns were enrolled in a randomized study of transurethral resection alone (53) or combined with intravesical BCG (102) as a treatmetn for superficial bladder cancer. BCG was administered for six consecutive weeks followed by three weekly instillations in months 3, 6, 12, 18, 24, 30 and 36 after resection. Recurrence, progression, prognostic factors and side-effects were assessed and analysed. Results. After a median (range) followup of 23 (6-42) months, 83 of the 102 patietns treated with BCG (81 p.c.) were disease-free, compared with 24 of the 53 treated with resection alone (45 p.c.). There was also a significant difference in tumour progression and time to progression between the trial arms. The disease progressed in eight patients (8 p.c.) treated with BCG and in 12 (23 p.c.) of those treated by resection alone. Independent risk factors for progression were DNA ploidy status and stage. Only the completion of treatment was predictive of outcome (risk of recurrence) for patietns treated with BCG. Conclusion. Maintenance BCG therapy was better than resection alone in reducing the incidence of recurrence and progression in patietns with high-risk superficial bladder cancer.

211/2523

Tytuł oryginału: Superficial bladder cancer T1G3: the choice of treatment.
Autorzy: Borkowski A[ndrzej]
Źródło: Br. J. Urol. 2002: 89 (6) s.623-627, tab., bibliogr. 41 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

212/2523

Tytuł oryginału: Multivariate inference in clinical trials - tests with model choice, confidence regions.
Autorzy: Luter J., Kropf S.
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.5-16, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In the paper, the formerly introduced spherical multivariate tests are coupled with procedures for selecting the relevant variables. These procedures work in such a way that a non-relevant set of variables is chosen with the probability of significance ŕ, at most. Furthermore, a new method of the calculation of linear principal-component scores is presented, which is based only on the within-sample covariances and yields, nevertheless, a level-ŕ test in each case. This method enables us to determine novel multivariate confidence regions of the unknown mean vector. The statistical procedures are demonstrated by a neurological example.

213/2523

Tytuł oryginału: Grade exploratory methods applied to some medical data sets.
Autorzy: Pleszczyńska Elżbieta, Szczęsny Wiesław
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.17-30, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Two sets of medical categorical data are analysed by means of grade exploratory methods, based on one fundamental procedure called the grade correspondence analysis (GCA). A special attention is put on visualization. The paper is a contribution to discussion concerning visualization of categorical data, which is going on in contemporary statistical literature.

214/2523

Tytuł oryginału: On the generalised affinity coefficient for complex data.
Autorzy: Bacelar-Nicolau Helena
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.31-42, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: This paper concerns the affinity coefficient and extensions for measuring the similarity between data units in classification, when we are dealing with large and complex databases. More precisely we refer to the extended weighted affinity coefficient and its role in hierarchical classification, when we are dealing with a generalised data table where the cells can contain a set of values, describing a probability distribution, a histogram (frequency distribution), or integer frequencies, for instance, instead one single value. Here we study the case of frequency distributions, since in our approach the other cases appear to be derived as a generalisation (integer frequencies, real data, for instance) or else as a particular case (binary data, ordinal data) of this one. Either the weighted affinity or the probablistic associated coefficients can be extended, in the clustering viewpoint, to hierarchical (and non-hierarchical) aggregation criteria and aggregation adaptive (parametric) families. An application to a real case is presented.

215/2523

Tytuł oryginału: Clinical databases orginating in electronic patients records.
Autorzy: Zvarova Jana
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.43-60, il., bibliogr. 16 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The paper is focusing on new technologies to standardize electronic documentation in Czech healthcare environment. Various approaches for collecting and stroing biomedical information using new information technologies are considered with respect to all legislative and standarization norms in the Czech Republic. Development of new approaches is based on experience gethered in the European 14C-TripleC project. New approaches for implementation of European ideas in the Czech healthcare environment are given. The focus on Czech healthcare, cultural and socio-economic factors in development of electronic healthcare record is presented. Special features of electronic healthcare record to serve in telematic applications for exploring electronic medical guidelines and medical decision support systems are discussed.

216/2523

Tytuł oryginału: Computer support of detecting risk of cardiovascular diseases.
Autorzy: Franek Petr, Tomeckova Marie, Zvarova Jana
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.61-68, il., tab., bibliogr. 9 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The paper is focusing on analysis of data collected in twenty years lasting longitudinal study of 1417 middle-aged men living in the second district of Prague. Examination of methods that can support estimation of the risk of cardiovascular disease development in time is done. These methods examined were neural networks, discriminant analysis and logistic regression. The final comparison was done according to percentage of correct decicions and using ROC curves.

217/2523

Tytuł oryginału: Physical and mathematical model of human hip joint load.
Autorzy: Włodarski Jerzy, Szmigiel Andrzej, Żołtyński Krystian, Kaczmarek Jan
Źródło: Biocybern. Biomed. Eng. 2002: 22 (1) s.95-102, il., bibliogr. 12 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Analysing human gait cyclogram and reactions of the foundation, kinematics and dynamics of loads realised on a test stand were tried to be brought closer to real object. To reconstruct hip joint load digram, basic loads resulting from physiology of movement were isolated. Physical model conditioned on gait cyclogram is designed to describe physical load of human hip joint and may be presented in the form of characteristic "double peak". Mathematical model of human hip joint load approximates with good accuracy time serious of five harmonics. This model has been adapted for the realisation of the design for a test stand - human hip joint simulator enabling to perform static and dynamic testing of an artificial hip joint.

218/2523

Tytuł oryginału: Vasopressinergic innervation of the pig pineal gland.
Autorzy: Przybylska-Gornowicz Barbara, Lewczuk Bogdan, Moller Morten
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.3-8, il., bibliogr. 30 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: An immunohistochemical study of the pineal gland of the domestic pig was carried out using the antisera raised against vasopressin (VP). The pineal glands were taken from the newborn, 21-day- and 7-month old female pigs. The pig pineal glands is moderately innervated by VP-immunoreactive nerve fibers. They run from the habenular commissure into the connective tissue septa and further into the pineal parenchyma. In the subependymal tissue as well as in the connective tissue septa, the fibres are smooth or with small varicosities and in the parenchyma with large ones. The obtained results point to extrapineal and extraepithalamic source of the fibres. The density of VP-immunoreactive fibers in the pineal gland of 7-month old pigs is higher than in the younger animals.

219/2523

Tytuł oryginału: Influence of 4-day long treatment with vasoactive intestinal peptide on ultrastructure and function of the rat pinealocytes in organ culture.
Autorzy: Nowicki Marcin, Lewczuk Bogdan, Przybylska-Gornowicz Barbara
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.9-16, il., bibliogr. 35 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Vasoactive intestinal peptide (VIP) is one neuropeptides involved in the regulation of the pineal gland function. The acute treatment of rat pinealocytes with VIP caused in their biochemical parameters. The present study concerns the effects of the chronic treatment with VIP on ultrastructure and function of the rat pinealocytes in organ culture. The pineals of adult male rats were assigned to one of three groups and placed in organ culture for four consecutive days. The pineals of the first group were incubated in the control medium, the pineals of the second group - 12 hrs in control medium and 12 hrs in medium with 1 ćM VIP (between 20.00 and 8.00) during each day, the pineals of the third group - 24 hrs per day in medium with 1 ćM VIP. The melatonin concentration was measured using RIA and activity of enzymes using radiochemical methods. Point count method was used in quantitative ultrastructural analysis. Both modes of chronic treatment with VIP increased significantly the level of melatonin secretion during four days of the culture and the content of this hormone in the pineal explants at the end of the experiment. Treatment with the neuropeptide for 12 hrs and 24 hrs per day elevated also the activity of arylalkylamine N-acetyltransferase and hydroxyindole=O-methyltransferase. On the other hand. VIP had no effect on the activity of arylamine-N-acetyltransferase. VIP increased the relative volume of rough endoplasmic reticulum. Golgi apparatus and mitochondria and did not influence the relative volume of lysosomes and lipid droplets as well as the numerical...

220/2523

Tytuł oryginału: Noradrenergic and peptidergic innervation of the mammary gland in the immature pig.
Autorzy: Franke-Radowiecka Amelia, Kaleczyc Jerzy, Klimczuk Magdalena, Łakomy Mirosław
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.17-25, il., tab., bibliogr. 41 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The presence and pattern of coexistence of some biologically active substances in nerve fibres supplying the mammary gland in the immature pig were studied using immunohistochemical methods. The substances studied included: protein gene product 9.5 (PGP), tyrosine hydroxylase (TH), somatostatin (SOM), neuropeptide Y (NPY), galanin (GAL), calcitonin gene-related peptide (CGRP) and substance P (SP). The mammary gland was found to be richly supplied by PGP-immunoreactive (PGP-IR) nerve fibres that surrounded blood vessels, bundles of smooth muscle cells and lactiferous ducts. The vast majority of these nerves also displayed immunoreactivity to TH. Immunoreactivity to SOM was observed in a moderate number of nerve fibres which were associated with smooth muscles of the nipple and blood vessels. Immunoreactivity to NPY occurred in many nerve fibres associated with blood vessela and in single nerves supplying smooth muscle cells. Solitary GAL-IR axons supplied mostly blood vessels. Many CGRP-IR nerve fibres were associated with both blood vessels and smooth muscles. SP-IR nerve fibres richly supplied blood vessels only. The colocalization study revealed that SOM, NPY and GAL partly colocalized with TH in nerve fibres supplying the porcine mammary gland.

221/2523

Tytuł oryginału: Immunohistochemical localization of the somatostatin receptor subtype 2A in the rat adrenal gland.
Autorzy: Pisarek Hanna, Pawlikowski Marek
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.27-30, il., bibliogr. 15 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The immunohistochemical localization of the somatostatin receptor subtype sst2A was investigated in the rat adrenal gland using SS-800 polyclonal antibody. The ss2A immunopositivity was found in all adrenocortical zones and in adrenal medulla, the reaction being slightly more intense in zona glomerulosa and medulla. The administration of the potent agonist of sst2 receptors - octreotide - resulted in the enhancement of the immunopositivity in zone glomerulosa and medulla, whereas chronic exposure of the rats to diethylstilbestrol led to enhancement of the immunopositivity in zone glomerulosa and in the external part of zona fasciculata.

222/2523

Tytuł oryginału: The effect of heat shock, cisplatin, etoposide and quercetin on Hsp27 expression in human normal and tumour cells.
Autorzy: Jakubowicz-Gil Joanna, Paduch Roman, Gawron Antoni, Kandefer-Szerszeń Martyna
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.31-35, il., bibliogr. 36 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Heat shock protein 27 (Hsp27) belongs to the group of proteins called molecular chaperones protecting normal and tumour cells against many stressors such as hyperthermia, several commonly used chemotherapeutics as well as other apoptotic stimuli. Our study was designed to determine whether heat shock and drugs like cisplatin, etoposide and quercetin have an effect on the expression of heat shock protein 27 in tumour cells such as: HeLa (cervical cancer), Hep-2 (larynx cancer), A549 (lung cancer) and also in normal human skin fibroblasts (HSF) cultured in two-dimensional (2D) and three-dimensional (3D) conditions. Our results indicate that Hsp27 expression is drug- and cell-type specific and depends on the culture model as well. HeLa, Hep-2 and HSF cell cultured in 3D model appeared to be more resistant to stimulatory or inhibitory effects of the applied drugs than cells cultured in 2D model. Only A549 cells cultured in 3D culture model appeared to be more susceptible and reacted to drugs and heat treatment with increased Hsp27 expression.

223/2523

Tytuł oryginału: Immunohistochemical assay of p53, cyclin D1, c-erbB2, EGFr and Ki-67 proteins in HPV-positive and HPV-negative cervical cancers.
Autorzy: Kędzia Witold, Schmidt Marcin, Frankowski Andrzej, Spaczyński Marek
Źródło: Folia Histochem. Cytobiol. 2002: 40 (1) s.37-41, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The purpose of the present study was to analyse clinical correlation between HPV type 16 and 18 infection, expression of p53, cyclin D1, Ki-67, c-erbB2 and EGFr gene products in cervical cancer cells as well as their nuclear ploidy. The morphological parameters evaluated, such as differentiation of carcinomas, vascular invasion, ploidy and expression of oncogenic proteins, indicate the increased biological malignancy of HPV 16/18-positive carcinomas. The majority of them were poorly differentiated revealed significantly higher frequence of vascular invasion (p 0.05), were more frequently aneuploid and showd overexpression of cyclin D1. The comparison of the data obtained with the mortality rate of the patients suggests that the overexpression of EGFr and moderate expression of Ki-67 seem to be unfavorable prognostic factors, regardless of the presence of HPV 16/18.

224/2523

Tytuł oryginału: Molecular basis of the pathogenicity of Proteus bacteria.
Tytuł polski: Molekularne podstawy chorobotwórczości bakterii z rodzaju Proteus.
Autorzy: Różalski Antoni
Źródło: Adv. Clin. Exp. Med. 2002: 11 (1) s.3-18, il., tab., bibliogr. 75 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie polskie: Bakterie Gram-ujemne z rodzaju Proteus, zaliczane do rodziny Enterobacteriaceae, są patogenami warunkowo-chorobotwórczymi, powodującymi m.in. zakażenia ran i układu moczowego (zakażenia dróg moczowych - urinary tract infections - UTI). Te ostatnie przebiegają z powikłaniami w postaci ostrego lub przewlekłego odmiedniczkowego zapalenia nerek i powstawania kamieni moczowych w pęcherzu i nerkach. Bakterie Proteus sp. są mikroorganizmami dimorficznymi, rosnąc na podłożu płynnym, stanowią krótkie pałeczki, tzw. komórki pływające, o długości 1 - 2 ćm, peritrychalnie urzęsione z 6 - 10 rzęskami na komórce. Po przeniesieniu na podłoże stałe komórki te różnicują się do długich form, wielkości 20 - 80 ćm, tzw. swarmer cells, z wieloma nukleoidami, nieprzedzielonymi przegrodami, ze zwiększoną nawet 500 razy liczbą rzęsek. Obie odmienne morfologicznie formy komórek Proteus, krótkie pałeczki i "swarmery" odgrywają rolę w chorobotwórczości, te ostatnie zwłaszcza w zakażeniach wstępujących, częściej powodowanych przez Proteus sp. Dotychczas zidentyfikowano następujące właściwości i czynniki chorobotwórczości tych bakterii: zjawisko rozpełzliwego wzrostu, fimbrie i glikokaliks odpowiedzialne za przyleganie, rzęski, właściwości inwazyjne, ureazę, deaminazy aminokwasów, proteazy, hemolizyny, polisacharyd powierzchniowy (CPS) i lipopolisacharyd (LPS, endotoksyna). Dyskutowane są ich genetyczna determinacja, podstawy molekularne i rola w chorobotwórczości Proteus sp.

Streszczenie angielskie: Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are opportunistic pathogens, which cause mainly wounds and urinary tract infections (UTI), the latter leading to severe complications, such as acute or chronic pyelonephrithis and formation of bladder and kidney stones. Proteus are dimorphic bacteria. When grown in a liquid medium, they are motile, peritrichously flagellated short rods, referred to as swimmer cells 1.0 to 2.0 ćm in length, with 6 - 10 flagella per cell. When transferred to a solid medium, Proteus short bacilli differentiate to swarmer cells, which are multinucleated, nonseptated cells 20 - 80 ćm in length containing even 500 fold more flagella. Both, short rods and long swarmer cells, are important in the pathogenesis of bacteria, the latter being particularly important in ascending UTI, which are more common for Proteus strains. Virulence factors and properties of Proteus sp. mediating infectious process are swarming phenomenon, adherence due to the fimbriae or glycocalyx, flagell, invasiveness, urease, amino acids deaminases, proteases, hemolysins, capsular polysaccharide (CPS), and lipopolysaccharide (LPS, endotoxin). The genetic determination, molecular basis, and the pathophysiological role of these factors in the infections caused by Proteus sp., are discussed.

225/2523

Tytuł oryginału: Late results of a phase-I trial for immunotherpay of pancreatic cancer.
Tytuł polski: Późne wyniki pierwszej fazy badań nad immunoterapią raka trzustki.
Autorzy: Waleczek Helfried, Lukenheimer Holger, Gerhards Roswitha, Werchau Hermann, Kozuschek Waldemar
Opracowanie edytorskie: Knast Witold (koment.).
Źródło: Adv. Clin. Exp. Med. 2002: 11 (1) s.19-24, il., bibliogr. 25 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Wprowadzenie. Na powierzchni komórek raka znajdują się antygeny swoiste dla rodzaju guza. W terapii nowotworu mogą więc znależć zastosowanie reakcje immunologiczne podobne do tych, jakie występują podczas procesu odrzucania przeszczepu. Wyniki leczenia raka trzustki są złe, nawet w przypadkach resekcji radykalnej. Terapia immunologiczna stanowi szansę na poprawę tych złych warunków. Cel pracy. Celem pracy była odpowiedź na pytanie, czy indywidualna odpowiedź immunologiczna może zostać pobudzona przez szczepionkę z zastosowaniem inaktywowancyh komórek raka zakażonych wirusem choroby Newcastle (NDV) pochodzących z autologicznych kultur komórek raka. Przedstawiono efekty kliniczne pierwszej fazy badań. Omówono również najnowsze piśmiennictwo poświecone badaniom z tej dziedziny (od 1995 r.). Materiał i metoda. Dziewięciu pacjentów zostało poddanych pełnej immunizacji po radykalnych operacjach z powodu raka trzustki wykonanych w latach 1992 - 1995. Hodowle komórkowe były prowadzone indywidualnie, jako kultury autologiczne, zostały zakażone NDV i inaktywowane odpowiednio do swej chemowrażliwości. Po zakończeniu pełnego szczepienia pacjentów miejsca odczynu skórnego na niezmodyfikowany materiał autologiczny guza wycinano i poddano badaniom immunohistochemicznym. Badania objęły okres do 2001 r. Wyniki. U wszystkich pacjentów stwierdzono indukcję lub wzmożenie nadwrażliwości typu późnego na autologiczny materiał guza. Reakcji tej nie odnotowano w stosunku do nieswoistych ...

Streszczenie angielskie: Background. Carcinoma cells present tumorspecific antigens on their cell surfaces. Thus immunological reactions similar to transplant rejections may be induced and used in tumor therapy. Results of therapy for cancer of the pancreas are still very poor even after complete resection. Immunological approaches may improve patients outcome. Objectives. The aim of the study was to determine whether an individual immunological response could be induced by vaccination with Newcastle Disease Virus (NDV) infected inactivated cancer cells from autologous tumor cell cultures. Clinical effects of a phase I trial were recorded. New insights since 1995 are discussed. Material and methods. 9 patients could be immunized completely after curative resection fo adenocarcinomas of the pancreas between 1992 and 1995. Tumors were grown individually as autologous cell cultures, NDV infected and inactivated according to their chemosensitivity. After complete vaccination patients reaction sites to unmodified autologous tumor material were resected and tested by immunohistochemistry. Clinical outcome up to 2001 was recorded. Results. All patients showed induction or inhancement of delayed type hypersensitivity to autologous tumor amterial but not to unspecific markers. No patient showed vaccination site skin metastasis development. No hepatic metastasis occurred in the vaccination group. 7 of these patients died due to local (retroperitoneal) tumor growth. 2 patients have no recurrent tumor ...

226/2523

Tytuł oryginału: GSTT1 and GSTM1 polymorphism in patients with the carcinoma laryngis.
Tytuł polski: Polimorfizm genów GSTT1 i GSTM1 u pacjentów chorych na raka krtani.
Autorzy: Schlade-Bartusiak Kamilla, Zych Monika, Kręcicki Tomasz, Sąsiadek Maria
Źródło: Adv. Clin. Exp. Med. 2002: 11 (1) s.25-27, tab., bibliogr. 14 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wprowadzenie. W etiologii dużą rolę odgrywają czynniki środowiskowe (głównie palenie papierosów i spożywanie alkoholu), ale jedynie u około 10 - 15 proc. palaczy występuje ten typ nowotworu. Zdeterminowana genetycznie podatność na powstawanie HNSCC może zależeć od aktywności metabolicznej enzymów detoksyfikujących karcinogeny obecne w dymie tytoniowym. Jedną z grup takich enzymów jest wielogenowa rodzina transferaz S-glutation. Geny GSTT1 i GSTM1 charakteryzują się polimorfizmem, który można wykryć, jeśli delecji ulegają obie kopie genu. Cel pracy. Celem pracy było zbadanie częstości delecji genów GSTT1 i GSTM1 u pacjentów z HNSCC w porównaniu z populacją kontrolną. Materiał i metody. Polimorfizm genów GSTT1 i GSTM1 badano technika PCR u 40 pacjentów chorych na raka krtani i u 77 osób z grupy kontrolnej. Wyniki. Nie stwierdzono znaczącej statystycznie różnicy między częstością delecji badanych genów u pacjentów z HNSCC w porównaniu z grupą kontrolną. Wnioski. Otrzymane wyniki sugerują brak wpływu delecji badanych genów na częstość występowania HNSCC.

Streszczenie angielskie: Background. Environmental factors (mainly cigarette smoking and alcohol consumption) play an important role in eatiology of head and neck squamous cell carcinoma (HNSCC), but only about 10 - 15 p.c. of heavy smokers develop this type of cancer. Genetically determined susceptibility to HNSCC may depend on the metabolic activity of enzymes that detoxify carcinogens present in tobacco smoke. One group of these enzymes is a multigene family of glutathione S-transferases. The GSTT1 and GSTM1 genes are known to be polymorphic. Polymorphic forms resulting from gene deletion are characterized by the lack of a detectable gene and are called null genotypes. Objectives. The aim of the present study was to investigate the frequency of GSTT1 and GSTM1 null genotypes in HNSCC patients in comparison to a control population. Material and methods. The PCR-based analysis of GSTT1 and GSTM1 polymorphism was done in the group of 40 cancer patients and 77 healthy controls. Results. Our results show no statistically significant difference in the distribution of GSTM1 and GSTT1 genotypes between the tested groups. Conclusions. The results are in agreement with the studies of other authors suggesting no involvement of the GSTM1 or GSTT1 null genotypes in the risk of HNSCC.

227/2523

Tytuł oryginału: Principal component analysis of patient variables as an objective method of treatment evaluation in adolescent idiopathic scoliosis.
Tytuł polski: Analiza składników głównych jako obiektywny sposób oceny leczenia skoliozy idiopatycznej.
Autorzy: Buciński Adam, Kowalski Ireneusz M., Zarzycki Daniel, Bączek Tomasz, Nasal Antoni, Kaliszan Roman
Źródło: Adv. Clin. Exp. Med. 2002: 11 (1) s.61-68, il., tab., bibliogr. 16 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Wprowadzenie. Analiza składników głównych (PCA - principal component analysis) została wykorzystana do oceny strategii terapeutycznej leczenia skoliozy idiopatycznej. Analiza PCA została przeprowadzona na dużym zbiorze wskaźników klinicznych. Metoda pozwoliła na ocenę złożonych zależności między zmiennymi charakteryzującymi pacjentów, chorobę i postępowanie terapeutyczne. Materiał i metody. Studia przeprowadzono na grupie 294 pacjentów, których charakteryzowały 137 zmienne. Wszystkich pacjentów poddano standardowej procedurze rehabilitacyjnej, przy czym 180 z nich było dodatkowo leczonych metodą elektrostymulacji LESS (lateral electrical surface stimulation). Macierz 137 zmiennych x 294 przypadki została poddana analizie składników głównych za pomocą programu Statistica (StatSoft Inc., Tulsa, OK, USA). Wyniki. Wyekstrahowane trzy składniki główne, PC1, PC2 i PC3 opisały 13 proc. całkowitej zmienności próby. Wyraźne zróżnicowanie położeń punktów w przestrzeni wyznaczonej przez trzy pierwsze składniki główne interpretowano w kategoriach podobieństw (różnic) między przypadkami. Wnioski. Wykazano, że postępowanie terapeutyczne włączające elektrostymulację LESS pozytywnie wpływa na leczenie skoliozy idiopatycznej.

Streszczenie angielskie: Background. An advanced multivariate data processing method, principal component analysis (PCA), was demonstrated to provide an objective evaluation of treatmetn strategy by making use of large collections of routine clinical parameters of adolescent idiopathic scoliosis patients. The method allowed for detection of complex systematic relationships between diverse patient, disease and treatment variables. Material and methods. Two hundred ninety four patients with adolescent idiopathic scoliosis were the subject of the study for whose one hundred thirty seven disease and treatment variables were determined. It was revealed by PCA that a lateral electrical surface stimulation (LESS) treatment positively affected the outcome of adolescent idiopathic scoliosis. A matrix of 294 x 137 data points was analyzed by PCA employing a commercially available statistical softwar and a personal computer. All the 294 patients underwent standard rehabilitation procedure and 180 of them were subjected additionally to LESS treatment. Results. Three principal components, PC1, PC2 and PC3, were extracted which accounted cumulatively for 13 p.c. of the variance of the data analyzed. An apparent clustering of the variables and a clear cut clustering of the patients were observed, which have been interpreted in terms of similarity (dissimilarity) of the variables and of the patients. Principal component analysis has been recommended as a new promising altlernative to classical regression analysis of multivariable clinical data. By means of PCA practically useful systematic information may be extracted from large sets of data otherwise hardly interpretable in comphrehensive physical terms...

228/2523

Tytuł oryginału: Remnants of respiratory peaks in heart rate variability spectrum of an adult man with tachypnea and bradycardia.
Tytuł polski: Pozostałości pików oddechowych w widmie zmienności rytmu serca dorosłego człowieka z szybkim rytmem oddechowym i wolnym rytmem serca.
Autorzy: Sacha Jerzy, Grzeszczak Władysław
Źródło: Folia Cardiol. 2002: 9 (1) s.67-74, il., bibliogr. 9 poz., streszcz.
Sygnatura GBL: 313,196

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: Analiza zmienności rytmu serca (HRV) w dziedzinie częstotliwości jest użytecznym narzędziem do badania fluktuacji rytmu zatokowego powodowanych przez autonomiczny układ nerwowy. Jednakże, nie jest jasne, jaka jest górna granica częstotliwości, która może być analizowana w widmie HRV. Materiał i metody: Badamy widma HRV dorosłego ochotnika z szybkim rytmem oddechowym, gdy jego rytm jest zwalniany bądź przyspieszany. Stosując szybką tranformatę Fouriera (FFT), analizujemy serie odstępów między uderzeniami serca w dziedzinie częstotliwość. Przeprowadzamy również komputerowe symulacje mierzonych sygnałów i porównujemy otrzymane z widmiami ochotnika. Wyniki: Jeżeli częstość oddechów przekracza połowę średniej częstości uderzeń serca, w widmie HRV są obecne dwa piki reprezentujące ten sam sygnał oddechowy. Jeden z pików jest wynikiem zjawiska aliasingu, natomiast drugi jest pozostałością prawdziwego piku oddechowego, jako efekt właściwości symetrii FFT. Żaden z nich nie przynosi rzetelnej, ilościowej informacji o fali oddechowej w widmie HRV. Wnioski: Górna granica częstotliwości w widmie HRV jest zdeterminowana przez połowę średniej częstotliwości serca. Przed obliczaniem widma należy więc określic górny możliwy do analizy limit częstotliwości, by uniknąć sytuacji, w której analizowane zakresy lokalizują się poza tą granicą. Sytuacja jest taka wielce prawdopodobna w przypadku, gdy pacjent wykazuje wolną akcję serca.

Streszczenie angielskie: Introduction: Heart rate variability (HRV) analysis in frequency domain is a useful tool to investigate fluctuations of the sinus rhythm caused by the nervous system. However, it is not clear what the upper limit of the frequency where the HRV spectrum is reliable is. Material and methods: We investigated HRV spectra of an adult man with high respiratory rate when his heartbeat rate was slowed down or accelerated. We applied Fast Fiurier Transform (FFT) to analyse the discrete event series in the frequency domain. We also performed numerical simulations of measured signals and compared the resulting spectra with those of the man. Results: It the respiration rate exceeds half of the average heartbeat rate, two peaks corresponding to the same respiratory signal can be found in the spectrum. One of them results from the aliasing phenomenon but the other is a remnant of the true respiratory peak whose origin lies in the symmetry properties of FFT. None of the peaks brings reliable, quantitative information about the respiration wave in the HRV spectrum. Conclusion: The upper frequency limit in a hRV spectrum is determined by half of the average heartbeat frequency. Consequently, one should check if any considered fluctuation lies within this frequency range, otherwise there is no reliable information about the fluctuation in the HRV spectrum. Such a situation is likely to occur when a patient exhibits slow heart rate.

229/2523

Tytuł oryginału: Developmental abnormalities in chicken embryos after injection proteolysis inhibitors from Ascaris suum.
Autorzy: Błaszkowska Joanna
Źródło: Wiad. Parazytol. 2002: 48 (1) s.55-64, il., tab., bibliogr. [33] poz.
Sygnatura GBL: 303,658

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zarodki

ptaki

Streszczenie angielskie: It has been found that trypsin and ŕ-chymotrypsin inhibitors isolated from Ascaris suum act embryotoxically and teratogenically on White Leghorn chicken embryos. Mortality rate for the chicken embryos on day 15 of incubation was 45.0 ń 3.5 p.c. after injection of trypsin inhibitor and 44.0 ń 3.5 p.c. after adminstration ŕ-chymotrypsin inhibitor. Gross examination of surviving embryos and their dissection revealed pathological changes (abdominal dropsy, umbilical hernia, subcutaneous oedema, hemoperitoneum, hemopericardium), symptoms indicating retardation in growth (lack of down, retarded ossification of long bones, decreased mean body weight) as well as malformations (schistocelia, micrognathia, cyclopia, crossed beak, cranial deformities) after injection of inhibitors from Ascaris. The highest incidence of embryos with pathological changes and malformationsd was foundf after administration of ŕ-chymotrypsin inhibitor. The most commonly occurring abnormality was schistocelia (21.4 ń 3.88 p.c.). Growth malformations were not found in the control groups. The trypsin and ŕ-chymotrypsin inhibitors present in Ascaris homogenate have a significant disturbing effect on ther development of the chicken embryo.

230/2523

Tytuł oryginału: Sprawozdanie z II Międzynarodowej Konferencji "A Sound fundation through early amplification", Chicago 6-10.XI.2001.
Autorzy: Obrębowski Andrzej
Źródło: Otolaryngol. Pol. 2002: 56 (2) s.275-276 - 2 Międzynarodowa Konferencja pt. Wczesna amplifikacja dżwięku Chicago 08-10.11. 2001
Sygnatura GBL: 313,466

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

sprawozdanie

tytuł obcojęzyczny

Wskaźnik treści:

231/2523

Tytuł oryginału: Hypoxic-ischemic encephalopathy: pathophysiology and implications for therapy.
Tytuł polski: Encefalopatia niedotlenieniowo-niedokrwienna u noworodków.
Autorzy: Sunshine Philip
Źródło: Prz. Lek. 2002: 59 supl. 1: Międzynarodowa konferencja "Postępy w neonatologii" s.6-9, bibliogr. 37 poz., streszcz. - Międzynarodowa konferencja pt. Postępy w neonatologii Kraków 18-20.04. 2002
Sygnatura GBL: 310,563

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

Streszczenie polskie: Encefalopatia niedotlenieniowo-niedokrwienna u noworodków wciąż jest ważnym problemem medycznym z uwagi na dużą śmiertelność, a także występujące u przeżywających niemowląt następstwa neurologiczne i upośledzenie intelektualne. Oprócz początkowej ostrej martwicy neuronów, wtórne ich uszkodzenie spowodowane reperfuzją dokonuje się przez 6 do 72 godzin od pierwotnego urazu. Wtórna niedomoga energetyczna komórek, rozpad błon komórkowych, dokomórkowy napływ jonów wapnia, produkcja cytokin oraz oksydacja przekaźnikowych aminokwasów - wszystko to przyczynia się do przyspieszonej i wzmożonej apoptozy neuronów. Nowe metody leczenia, takie jak stosowanie inhibitorów wolnych rodników tlenowych oraz utrzymywanie przez pierwsze 72 godziny życia łagodnej lub umiarkowanej hipotermii ogólnoustrojowej lub wybiórczej hipotermii głowy są obiecującymi środkami pomocniczymi w postępowaniu z noworodkami z encefalopatią niedotlenieniowo-niedokrwienną.

Streszczenie angielskie: Hypoxic-ischemic encephalopathy continues to be a major problem in perinatal medicine because of the high mortality rate and neurological and intellectual imparment in the surviving infants. In addition to the acute necrotic damage that occurs in the neurons initially, reperfusion injury and secondary neuronal damage continue for 6 to 72 hours after the initial insult. Secondary cellular energy failure, membrane breakdown, cellular influx of calcium ions, elaboration of cytokines, and oxidation of excitory amino acids all contribute to enhanced apoptosis of the neurons. Newer forms of therapy including the use of oxygen-free radical inhibitors and mild to moderate cerebral or systemic hypothermia for 72 hours following the asphyxial period are promised adjuncts as follow-up approaches to the affected newborns.

232/2523

Tytuł oryginału: Status report: inhaled nitric oxide in persistent pulmonary hypertension/hypoxic respiratory failure of neonate.
Tytuł polski: Zastosowanie tlenku azotu w leczeniu zespołu zaburzeń oddychania oraz przetrwałego nadciśnienia płucnego noworodków.
Autorzy: Cook Larry N.
Źródło: Prz. Lek. 2002: 59 supl. 1: Międzynarodowa konferencja "Postępy w neonatologii" s.10-13, bibliogr. 50 poz., streszcz. - Międzynarodowa konferencja pt. Postępy w neonatologii Kraków 18-20.04. 2002
Sygnatura GBL: 310,563

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

Streszczenie polskie: Przetrwałe nadciśnienie płucne noworodków (PPHN) może być obserwowane w przebiegu różnych zaburzeń, w tym m. in. u dzieci z ciężką zamartwicą, zespołem aspiracji smółki, posocznicą wywołaną przez paciorkowce grupy B lub też u chorych z wadami wrodzonymi jak np. wrodzoną przepukliną przeponową. W pierwszej części omówiono stosowanie standardowych metod leczenia jak również użyteczność podawania surfaktantu i wentylacji wysokimi częstotliwościami u noworodków z PPHN. Następnie przedstawiono zasadę działania wziewnego tlenku azotu (iNO) oraz oceniono wyniki badń klinicznych na temat skuteczności tlenku azotu u noworodków z PPHN. Omówiono nowe kierunki badawcze, w tym stosowanie iNO u wcześniaków z zespołem zaburzeń oddychania oraz możliwości wykorzystanmia iNO w profilaktyce przewlekłej choroby płuc u wcześniaków. Przedstawiono aktualne zalecenia na temat dawkowania iNO u noworodków oraz aktualnie opublikowane wytyczne Amerykańskiej Adakemii Pediatrii na temat stosowania iNO u noworodków. W ostatniej części dokonano przeglądu dostępnych badań klinicznych oceniających odległe wuniki leczenia iNO u noworodków.

Streszczenie angielskie: Presistent pulmonary hypertension is seen in association with a number of diseases and conditions in the newborn including perinatal asphyxia, meconium aspiration syndrome, Group B strep sepsis, and certain surgical conditions such as congenital diaphragmatic hernia. The conventional therapy of persistent pulmonary hypertension is discussed as well as the adjunctive role of surfactant replacement therapy and high frequency ventilation. The mechanisms of action of inhaled nitric oxides as a selective pulmonary vasodolator is explained. Human neonatal clinical experience is chronicled from the original studies of Roberts in 1992 and Kinsella in 1993. A review of three large prospective randomized trials of nitric oxides is presented as well as a summary of new areas of investigation including the use of nitric oxide in pre-term infants and the use of nitric oxide in the prevention of chronic disease. Current dosing recomendations are presented as well as guidelines recently published from the American Academy of Pediatrics. A review of follow up literature in relationship to patients receiving inhaled nitric oxides is also summarized.

233/2523

Tytuł oryginału: T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia - the three-color flow cytometry analysis.
Autorzy: Podhorecka Monika, Dmoszyńska Anna, Roliński Jacek, Wąsik Ewa
Źródło: Leuk. Res. 2002: 26 (7) s.657-660, il., bibliogr. 8 poz.
Sygnatura GBL: 306,151

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The impairments in immune cell functions as well as changes in cytokine network are the cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic limphocytic leukemia (B-CLL). To broaden the knowledge of immune system in B-CLL we tried to evaluate the Th1/Th2 and Tc2 balance in B-CLL patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNç expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells. The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression.

234/2523

Tytuł oryginału: Morphine attenuates pain and prevents inflammation in experimental peritonitis.
Autorzy: Płytycz Barbara, Natorska Joanna
Źródło: Trends Immunol. 2002: 23 (7) s.345-346, il., bibliogr. 13 poz.
Sygnatura GBL: 312,774

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

235/2523

Tytuł oryginału: Primenenie mediastinoskopii w diagnostike khirurgicheskikh zabolevanij organov grudnoj polosti.
Tytuł angielski: Application of mediastinoscopy in diagnosis of surgical diseases of the thoracic cavity organs.
Autorzy: Pieluchowski S. W.
Źródło: Klin. Khir. 2002 (1) s.58-59, bibliogr. 5 poz.
Sygnatura GBL: 303,753

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

236/2523

Tytuł oryginału: Oslozhnenija posle operacij s primeneniem videotorakoskopii i ikh lechenie.
Tytuł angielski: Complications after operation with videothoracoscopy application and their treatment.
Autorzy: Pieluchowski S. W., Piwkowskij C., Kasprzak P.
Źródło: Klin. Khir. 2002 (2) s.30-31, bibliogr. 6 poz., sum.
Sygnatura GBL: 303,753

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Of 4715 patient, who were present in 1995-2000 yrs in the clinic, in 708 (15 p.c.) operation, using videothoracoscopy method, was performed. In 539 (76.1 p.c.) of patietns the method was applied for diagnosis and in 169 (23.9 p.c.) - for treatment. After the operation 365 patients were observed, complications have occurred in 14, including the early - in 10 (2.7 p.c.) and the late - in 4 (1.1 p.c.). All the patients have survived.

237/2523

Tytuł oryginału: Analysis of Ixodes ricinus (L.) tick burdens in a resident passerine bird community in the Mazurian Lake region (Northeastern Poland).
Autorzy: Gryczyńska Alicja, Barkowska Miłosława, Siemiątkowski Marek
Źródło: Acta Parasitol. 2002: 47 (1) s.51-57, il., tab., bibliogr. [32] poz.
Sygnatura GBL: 303,018

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The abundance of birds and their tick parasites were estimated in a residential avian community located in the Mazurian Lake region (NE Poland). A total of 1624 passerine birds (representing 45 species) were captured, of which 25 p.c. were infested with at least one tick. All the ticks belonged to the species Ixodes ricinus. The highest tick infestation prevalence ( 50 p.c.) were recorded for dunnock (Prunella modularis), tree pipit (Anthus trivialis), hawfinch (Coccothraustes coccothraustes) and blackbird (Turdus merula). Changes in tick infestation prevalence of passerine birds are seasonal. June and September were the two months in which tick infestation rates were the highest. The percentage of birds that were tick carriers was significantly greater in mixed coniferous forest than in alder swamp forest (respectively 32 p.c and 20 p.c. of birds were infested with ticks).

238/2523

Tytuł oryginału: Experimental Chagas'disease: the protection induced by immunization with Trypanosoma rangeli is associated with down-regulation of IL-6, TNF-ŕ and IL-10 synthesis.
Autorzy: Cervetta Liliana, Moretti Edgardo, Basso Beatriz
Źródło: Acta Parasitol. 2002: 47 (1) s.73-78, il., bibliogr. [36] poz.
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In an experimental model developed in our laboratory, BALB/c mice were protected against Trypanosoma cruzi infection by immunization with T. rangeli, a parasite closely related to T. cruzi but not pathogenic for man. Immunized mice developed low parasitemia and high survival rates when challenged with T. cruzi. In the present work, we study the patterns of systemic production of interleukin (IL-6), tumor necrosis factor alfa (TNF-ŕ), interferon gamma (IFN-ç), IL-2, IL-4 and IL-10 during the acute phase of T. cruzi infection in mice previously immunized with T. rangeli with respect with respect to only infected mice. The results showed a concentration of IL-6, TNF-ŕ and il-10 significantly lower in immunized mice than in only infected ones. The production of IFN-ç, a cytokine involved in resistance, was preserved in vaccinated mice. Finally, both groups of animals showed IL-2 and IL-4 serum levels similar to those observed in uninfected mice. As in other experimental or human infections, in this model, the pattern of systemic production of cytokines can be associated with the outcome of infection.

239/2523

Tytuł oryginału: A new automatized fluorometric assay for anti-Leishmania drug screening.
Autorzy: Le Pape Patrice, Pagniez Fabrice, Abdala Hiam
Źródło: Acta Parasitol. 2002: 47 (1) s.79-81, il., tab., bibliogr. [11] poz.
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The therapeutic armentarium against leishmaniasis is very restricted, using mainly up to now drugs showing several side effects and resistance. Consequently, there is a real need to find new compounds as alternatives for the treatment of leishmaniasis. However, most of the classic antileishmanial primary screening assays are not suitable for measuring the cytotoxicity of large number of drug candidates because of the manipulation required. we have established a new assay that incorporate a fluorometric growth indicator based on the detection of a metabolic activity in culture medium after the chemical reduction of alamar Blue by cells. This antileishmanial test was evaluated on amphotericin B. meglumine antimoniate, allopurinol, ketoconazole and edelfosine. The results reported show the advantages of this fluorochrome on the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) for Leishmania cytotoxicity measurement. Moreover, this study is the first report of the use of alamar Blue flurogenic assay for activity assessment of potential antileishmanial drugs against promastigotes.

240/2523

Tytuł oryginału: Propranolol and metoprolol enhance the anticonvulsant action of valproate and diazepam against maximal electroshock.
Autorzy: Luchowska Elżbieta, Luchowski Piotr, Wielosz Marian, Kleinrok Zdzisław, Czuczwar Stanisław J., Urbańska Ewa M.
Źródło: Pharmacol. Biochem. Behav. 2002: 71 (1/2) s.223-231, il., tab., bibliogr. [40] poz.
Sygnatura GBL: 312,498

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

myszy

Streszczenie angielskie: The anticoconvulsive potential of classical antiepileptics co-administered with á-adrenergic receptor antagonists against generalized tonic-clonic seizures was evaluated in the model of maximal electroshock (MES)-induced convulsions. Propranolol, acebutolol, metoprolol and atenolol were tested in the doses not affecting the electroconvulsive threshold. Propranolol and metoprolol lowered the ED50 of valproate and diazepam. Acebutolol reduced valproate's but not diazepam's ED50 value. In contrast, hydrophilic atenolol, not penetrating via blood-brain barrier, affected neither the action of valproate nor diazepam. None of the studied drugs changed the protective activity of carbamazepine and phenytoin against MES. á-blokers per se did not alter the motor performance of mice. Moreover, propranolol and metoprolol did not influence diazepam-evoked impairment of locomotor activity. The free plasma and brain levels of antiepileptic drugs were not affected by á-blokers. In conclusion, the use of certain á-adrenoceptor antagonists, such as propranolol and metroprolol, might improve the antiepileptic potential of valproate and diazepam.

241/2523

Tytuł oryginału: Sarcal nerve modulation for fecal incontinence. Functional results and assessment of the quality of life.
Tytuł polski: Pobudzenie nerwów krzyżowych w leczeniu nietrzymania stolca. Ocena wyników czynnościowych i jakości życia.
Autorzy: Ganio E[nzo], Luc Realis A., Ratto C., Doglietto G. B., Masin A., Dodi G., Altomare D. F., Memeo V., Rippeti V., Arullani A., Falletto E., Gaetini , Scarpino O., Saba V., Infantino A., La Manna S., Ferulano , Villani R.
Źródło: Proktologia 2002: 3 (2) s.121-126, il., bibliogr. 13 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Bezpośrednia stymulacja nerwu sromowego w sytuacji morfologicznie nieuszkodzonych zwieraczy odbytu stanowi atrakcyjną możliwość leczenia nietrzymania stolca, pozwalającą wykorzystać istniejący aparat zwieraczowy. Oceniano metodę bezpośredniej stymulacji korzeni krzyżowych pod kątem wykonalności, bezpieczeństwa, skuteczności i jej wpływu na jakość życia pacjentów. Badaniem objęto 116 pacjentów z nietrzymaniem stolca stałego i płynnego przynajmniej raz w tygodniu przez co najmniej 2 tygodnie, nie reagujących na leczenie konwencjonalne. W znieczuleniu miejscowym wprowadzono igłę do nakłucia lędźwiowego, którą następnie zastępowano czasowym przewodem stymulatora. Stymulacja nerwów powodowała ściśle określoną reakcję ruchową w zakresie krocza, zwieraczy, a także ruch nogi. U pacjentów poddanych stymulacji zaobserwowano poprawę funkcji zwieraczy, ze zmniejszeniem się liczby epizodów nietrzymania średnio z 11 do 3. Wśród 36 osób zakwalifikowanych do implantacji stymulatora na stałe liczba epizodów nietrzymania zmniejszyła się średnio z 15 do 3,3 w okresie 14 dni. Manometria anorektalna nie wykazała statystycznie znamiennych różnic. Uzyskano także poprawę w zakresie drobnych epizodów nietrzymania (popuszczanie gazu albo brudzenie bielizny), ze zmniejszeniem się ich liczby z 41,6 do 4,2. Wyniki tego badania dowodzą, że w wybranych przypadkach neuromodulacja krzyżowa stanowi nową i skuteczną metodę leczenia nietrzymania stolca. Po zastosowaniu symulatora stwierdzono również statystycznie znamienną korelację między poprawą wskaźników zdrowia psychicznego i somatycznego oraz poprawę stanu czynnościowego zwieraczy.

Streszczenie angielskie: Direct stimulation of the pudendal nerve in a morphologically intact anal sphincter is an attractive treatment option as it allows use of the anatomical existing sphincters. Method of direct stimulation of the sacral roots was evaluated according to feasibility, safety, effectiveness and its impact on the patient's quality of life. The study included 116 patients with incontinence to solid and liquid stool once a week for at least 2 preceding weeks, which did not respond to conventional treatments. A spinal needle was inserted under the local anesthesia or temporary stimulator lead was used to replace it. Stimulation of nerves resulted in specific motor responses of the perineum and sphincters along with movement of the leg. Among the patients who underwent perineal nerve evaluation, an improvement was observed in continence, with a decrease in the number of incontinent episodes from mean of 11 to 3 episodes. Of 36 who were selected for definitive implant incontinence episodes decreased from 15 to 3.3 per 14 days. Anorectal manometry did not show any statistical difference. Improvement in the episodes of minor incontinence (to gas and soliting) was also seen with reduction from 41.6 episodes to 4.2. The study demonstrates that sacral neuromodulation in selected patients represents a new and effective tool in treatment. There was also a significant correlation between the mental and physical health indices and continence status after the implant.

242/2523

Tytuł oryginału: Combined parasacral and perineal approach in the surgical treatment of the anal incontinence.
Tytuł polski: Skojarzone dojście kroczowe i okołokrzyżowe w leczeniu chirurgicznym nietrzymania stolca.
Autorzy: Korcek J., Bakos E., Klobusicky P.
Źródło: Proktologia 2002: 3 (2) s.127-132, il., tab., bibliogr. 14 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Na podstawie własnych doświadczeń anatomicznych i klinicznych, a także doświadczeń związanych z leczeniem chirurgicznym nietrzymania stolca o różnej etiologii autorzy wykazali skuteczność i praktyczną przydatność skojarzonego dojścia przykrzyżowo-kroczowego (CPAPP) w chirurgicznym leczeniu nietrzymania stolca. Autorzy przeprowadzili powtarzane pomiary końcowej latencji ruchowej nerwu sromowego (PN TML) u 23 pacjentów z wydłużeniem latencji przed zabiegiem. Po operacji u 82,6 proc. pacjentów uzyskano prawidłowe wyniki PN TML, co korelowało z pełną sprawnością zwieracza.

Streszczenie angielskie: In accordance with their own anatomic and clinical experiences as well as the experiences from the surgical therapy of various kinds of anal incontinence, the authors prove the expedience and effectiveness of the combined parasacral and perineal approach (CPAPP) in the surgical therapy of the anal incontinence. Authors performed a repetitive measuring the pudendal nerve terminal motor latency (PN TML) in the group of 23 patients with the pre-operative prolonged PN TML. 82.6 p.c. of the patients had postoperatively the normal values PN TML and this was in the correlation with the full continence.

243/2523

Tytuł oryginału: Pilonidal diseases: a review.
Tytuł polski: Torbiel włosowata kości ogonowej. Przegląd.
Autorzy: Casula G., Zorcolo L., Capra F., Scintu F.
Źródło: Proktologia 2002: 3 (2) s.133-139, tab., bibliogr. 80 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Zatoka włosowata jest to ślepo zakończony przewód, o ścianach zbudowanych z ziarniny, zakończony jamą zawierającą włosy i złuszczony nabłonek. Może ona powodować znaczne kłopoty głównie u osób młodych, zarówno w skutek dyskomfortu, jak i straconego czasu pracy. Proponowano wiele różnych sposobów leczenia zatoki włosowatej, jednak większość z nich obarczona jest znacznym odsetkiem nawrotów. Są to różne metody - od prostego przyżegania, przez wstrzyknięcie fenolu, do złożonych zabiegów plastycznych. Największe prawdopodobieństwo powodzenia mają zabiegi chirurgiczne omijające linię środkową. Metoda Bascoma jest najprostszym sposobem uzyskania rany głównej poza linią środkową. Technika ta jest prosta i nadaje się do stosowania w warunkach "chirurgii jednego dnia" w znieczuleniu miejscowym. Daje też korzystne wyniki pod względem szybkiego powrotu do pracy i niewielkiego odsetka nawrotów. Autorzy uważają metodę Bascoma za metodę z wyboru w początkowym leczeniu objawowej zatoki włosowej. Asymetryczne wycięcie z pierwotnym zamknięciem skóry (zabieg Karidakiego) również stanowi korzystną alternatywę. Może być przeprowadzony w znieczuleniu miejscowym, jednak wiąże się z nieco większym odsetkiem powikłań i jest technicznie trudniejszy, ale ryzyko nawrotu jest tu mniejsze. Bardziej definitywna metoda, np. plastyka Z, jest zalecana u pacjentów z nawracającą i nie gojącą się raną w linii środkowej lub z nawracającą zatoką włosowatą.

Streszczenie angielskie: Pilonidal sinus is blind-end track lined with granulation tissue, which leads to a cavity containing hairs and skin debris. It is a considerable source of problems in young patients both in terms of discomfort and time of work. Many procedures have been proposed for its management but most of them carry substantial persistence/recurrence rates. Treatments vary from simple cauterization with fenol injection to complex plastic procedures. Surgical procedures avoiding a wound in the midline are most likely to succeed. Bascom's technique is the simplest method to keep the main wound away from the midline. The technique is simple and suitable for one-day surgery in local anasthesia. It also gives favorable results in terms of return to work and rate of recurrence. Authors consider Bacom's technique the procedure of choice in the initial treatment of symptomatic pilonidal disease. Asymmetric excision with primary closure (Karidaki's procedure) is also an attractive option. It can performed under local anasthesia, has a little higher complication rate and is more complex, however is characterized by a lower risk of reccurence. More definitive treatments such as Z-plasty is probably the best choice in patients with persistently unhealed midline wound or recurrent pilonidal sinus.

244/2523

Tytuł oryginału: Local excision of rectal cancer.
Tytuł polski: Miejscowe wycięcie raka odbytnicy.
Autorzy: Rosa G., Lolli P., Piccinelli D., Bonomo S., Brugnettini F.
Źródło: Proktologia 2002: 3 (2) s.140-142, bibliogr. 9 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Miejscowa resekcja jest możliwa jedynie u ok. 5 proc. pacjentów z rakiem odbytnicy, mimo widocznego ostatnio wzrostu zaintresowania tą metodą związanego z rozwojem nowych technik operacyjnych (m.in. TEM), rozszerzających możliwości działania chirurga i umożliwiających operowanie guzów zlokalizowanych nawet ponad 10 cm od brzegu odbytu. Kluczową sprawą pozostaje właściwe określenie wskazań, co z kolei determinuje wybór rodzaju operacji. Wskazania do miejscowej resekcji guza to: średnica guza nie przekraczająca 3 cm, guz egzofityczny (wyrasta do światła kanału odbytu), brak owrzodzeń, guz nie jest nieruchomy względem otoczenia, o niskiej złośliwości, zlokalizowany do 8 cm od brzegu odbytu (przy zastosowaniu metody tradycyjnej) albo do 12 cm na ścianie tylnej i do 15 cm na ścianie bocznej (przy zastosowaniu techniki TEM). Autorzy przeprowadzili 60 zabiegów miejscowej resekcji, z których 41 było zabiegiem definitywnym i dającym pełne wyleczenie. Pacjentów poddano ścisłej obserwacji przez 18 miesięcy, wykonując badania proktologiczne, proktoskopię, ultrasonografię transonalną oraz, w razie potrzeby, badania CT i MRI, a także rutynowe badania hematologiczne stosowane w onkologii. Dobre wyniki leczenia zależą od prawidłowego wyboru pacjentów z nowotworem w stadium T1, należących do grupy niskiego ryzyka.

Streszczenie angielskie: Local excision remains a limited practice concerning only 5 p.c. of rectal cancers despite the fact that it has been given a new attention with new instruments (TEM) to extended the range of action allowing to reach neoplasms located over 10 cm from anus. The main issue remains correct indications, which determines the operation. Elective indications for local excision include: diameter of the tumor less than 3 cm, tumor has to be exofitic, non-ulcerated, non-fixed, low malignancy, located up to 8 cm from anus in case of traditional and up to 12 cm on the posterior, 15 on the lateral by TEM techniques. Authors performed 60 local excisions. 41 of them were considered definitive and curative. Close follow up for 18 months include proctologic examination, proctoscopy, transanal ultrasound, CT, MRI if necessary, hematological oncological routine. Good results depend on rigorous selection of patients with T1 at lower risk.

245/2523

Tytuł oryginału: Ambulatory surgery - what we can be done on a one day surgery basis in proctology?
Tytuł polski: Chirurgia jednego dnia - jakie zabiegi mogą być wykonywane z zakresu proktologii?
Autorzy: Kosorok Pavle, Pokrajac Tatjana
Źródło: Proktologia 2002: 3 (2) s.143-147, il., bibliogr. 16 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Wstęp: W naszej instytucji nabyliśmy wiele doświadczeń w dziedzinie proktologii. Siedem lat temu wprowadziliśmy do naszej praktyki "chirurgię jednego dnia" i uzyskaliśmy bardzo dobre wyniki leczenia praktycznie u wszystkich pacjentów wymagających chirurgicznego leczenia chorób proktologicznych. Materiał, metoda i wyniki: W tym okresie do leczenia chirurgicznego zakwalifikowano 6126 spośród 41913 pacjentów leczonych ambulatoryjnie . W grupie tej było 23,1 proc. pacjentów operowanych z powodu krwiaka okołoodbytowego. Uzyskaliśmy też pewne doświadczenia w zakresie drobnych zabiegów w okolicy odbytu (usunięcie wyrośli skórnych u 17,0 proc. pacjentów, wycięcie brodawek u 5,3 proc. pacjentów); pacjenci z chorobą hemoroidalną, operowani klasyczną metodą Milligana-Morgana (10,46 proc.) albo nową metodą PPH wg Longo (1,9 proc.). Leczono też 11,7 proc. pacjentów z przetoką okołoodbytniczą, stosując fistulotomię lub wycięcie przetoki, oraz 10,9 proc. pacjentów z drobnymi problemami chirurgicznymi (operacje włókniaków, torbieli naskórzastych, brodawek, ganglionów, tłuszczaków itp.). Usunęliśmy polipy odbytu i odbytnicy u 7,6 proc. pacjentów, torbiele przywłośne u 6,1 proc., leczyliśmy także 1,3 proc. pacjentów z ropniem odbytu. Wykonaliśmy beznapięciową plastykę przepukliny pachwinowej u 4,5 proc. pacjentów., a także korekcji stomii u 0,14 proc. pacjentów. Wnioski: W naszym doświadczeniu operacje w warunkach "chirurgii jednego dnia" stanowią właściwy sposób leczenia wielu ...

Streszczenie angielskie: Introduction: In our institution we've had a lot of experience in proctology. Seven years ago we introduced one day surgery in our practice and had really good results in treating practically all patients who needed proctologic surgery. Material, methods and results: During this period of time 6,126 out of 41,913 outpatient cases were chosen for surgery. Among them, 23.1 p.c. were patients with operations of perinal haematomas. We had some experiences with minor anal surgery (excisions of skin tags in 17.0 p.c. patients, excisions of papillas in 5.3 p.c. patients); patients with haemorrhoids, operated classically on Milligan-Morgan procedure (10.46 p.c.) or by the new PPH - Longo procedure (1.9 p.c.). We also treated 11.7 p.c. patients with fistulas - either with fistulotomy or excision of fistulae - and 10.9 p.c. patients with minor surgery (operations of fibromas, verrucas, ganglions, lipomas, etc.). We made excisions of anorectal polyps in 7.6 p.c. patients, excisions of pilonidal cysts in 6.1 p.c. patients and treated 1.3 p.c. patients with anal abscesses. We made tension free operations of groin hernias in 4.5 p.c. patients and corrections of stomas in 0.14 p.c. patients. Conclusions: We have experienced ambulatory surgery (i.e. day surgery hospital) as an appropriate choice for treating the patients with proctologic pathology. However, it used to be the practice to treat all these patients in hospitals, where they occupied occupied bed facilities that could be ...

246/2523

Tytuł oryginału: New modalities in the treatment of haemorrhoids.
Tytuł polski: Nowe metody leczenia choroby hemoroidalnej.
Autorzy: Bielecki Krzysztof
Źródło: Proktologia 2002: 3 (2) s.149-151, 153-154, tab., bibliogr. 15 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: W pracy omówiono leczenie choroby hemoroidalnej ze szczególnym uwzględnieniem nowych metod. Przedstawiono technikę i wyniki leczenia. Dokładnie omówiono wskazania i wyniki po stosowaniu staplerowej hemoroidektomii w odmianach Longo i Stram. Najbardziej rekomendowaną przez autora jest Doppler-duided haemorrhoidal artery ligation, która wydaje się być najmniej bolesną techniką leczenia.

Streszczenie angielskie: In this paper various treatment options of haemorrhoidal disease are discussed, with special emphasis on new methods. Particular techniques and treatment results are presented. Indications and results of stapled haemorrhoidectomy by the Longo and the STRAM methods are discussed in detail. The author recomends the Doppler-guided haemorrhoidal artery ligation, which appears as the least traumatic and painful treatment technique.

247/2523

Tytuł oryginału: Indications and possibilities of semi-invasive treatment of hemorrhoids.
Tytuł polski: Wskazania i możliwości półinwazyjnego leczenia choroby hemoroidalnej.
Autorzy: Skricka Tomas, Vedra Pavel
Źródło: Proktologia 2002: 3 (2) s.155, 157-161, il., bibliogr. 17 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Prawie 70 proc. osób dorosłych po 30. roku życia cierpi z powodu hemoroidów, mając objawy takie, jak krwawienie, ból i miejscowy odczyn zapalny. W tradycyjnym ujęciu nasilenie choroby określa się wg 4-stopniowej skali, zależnie od zakresu wypadania żylaków odbytu. W pierwszym etapie stosuje się leczenie farmakologiczne, ukierunkowane na zwalczanie krwawienia, bólu i podrażnienia, a także likwidację stanu zapalnego. Zaleca się miejscowe stosowanie środków antyseptycznych i antybiotyków, aby uniknąć miejscowego zakażenia. Metody półinwazyjne obejmują skleroterapię, krioterapię, fotokoagulację i podwiązanie gumowymi taśmami. W przypadku niewielkich hemoroidów, powodujących głównie krwawienie bez wypadania śluzówki, leczeniem z wyboru jest skleroterapia. Obecnie praktycznie zaprzestano wykonywania zabiegów krioterapii z uwagi na dużą bolesność i krwawienie. W przypadku hemoroidów pierwszego stopnia godną polecenia metodą jest fotokoagulacja. Zakładanie podwiązek gumowych pozwala uzyskać wyniki równie dobre jak przy zastosowaniu metod tradycyjnych, jednak zaletą podwiązek jest możliwość stosowania ich w warunkach ambulatoryjnych bez znieczulenia miejscowego. Metoda uważana jest za najlepszy i najbezpieczniejszy półinwazyjny sposób leczenia hemoroidów, nie tylko w przypadku choroby w drugim stopniu zaawansowania. Kombinacja diety, dbałości o prawidłowe wypróżnienia, farmakoterapię i leczeniapółinwazyjnego pozwala uzyskać dobre wyniki u ponad 90 proc. pacjentów.

Streszczenie angielskie: Almost 70 p.c. of adult population over 30 suffer from haemorrhoids with symptoms of bledding, pain and inflammation. Traditional grading divides into four degrees depending on the extent of prolaps. First choice of treatment is drug therapy, which has to include therapy of bleeding, pain and irritation as well as anti-inflammatory. The use of antiseptics and antibiotics should be used by preference to guard against local infection. Semi-invasive therapy includes sclerotherapy, cryotherapy, fotocoagulation and rubber-band ligation. The sclerotherapy is the first line of treatment in minor degree heamorrhoids particularly those causing bleeding without mucosal prolapse. Cryotherapy is practically abandoned on the ground of pain and profuse discharge. Photocoagulation is good and sufficient method of treatment in the first-degree haemorrhoids. Rubberband ligation enables to achieve results as valid as those of traditional methods in the treatment of haemorrhoids with the advantage of employing it in out-patient development with no need for local anasthesia. It is considered to be the best and most safe semi-invasive method of treatment not only of the second-degree haemorrhoids. Combination of diet, improvement of emptying, pharmacotherapy and semi-invasive treatment can treat more that 90 p.c. patients successfully.

248/2523

Tytuł oryginału: Rectal cancer - functional outcome and quality of life.
Tytuł polski: Rak odbytnicy - czynnościowe wyniki leczenia i jakość życia.
Autorzy: Szczepkowski Marek
Źródło: Proktologia 2002: 3 (2) s.162-174, tab., bibliogr. 25 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Celem pracy było przedstawienie jakości życia pacjentów po operacjach z powodu raka odbytnicy. Przedstawiono analizę porównawczą pacjentów po brzuszno-kroczowej amputacji odbytnicy i chorych po przedniej niskiej resekcji z zachowaniem zwieraczy. W ocenie jakości życia chorych brano pod uwagę następujące elementy: a) funkcjonowanie organizmu i ogólny stan zdrowia (physical functioning); b) stan psychologiczny (psychologic functioning); c) sfera socjalna (praca zawodowa, relacje z osobami bliskimi - rodzina, znajomi, model wypoczynku i rozrywki) (social functioning); d) życie intymne (sexual functioning). W obu grupach pacjentów, zarówno po brzuszno-kroczowym odjęciu odbytnicy, jak i po przedniej niskiej resekcji odbytnicy, występuje znaczące obniżenie jakości ich życia. Pomimo że ogólny stan zdrowia pacjentów w obu grupach w większości przypadków jest dobry (u ponad 60 proc.), nieregularne wypróżnienia i biegunka występują często. Pacjenci ze stomią częściej zgłaszają problemy związane z niekontrolowanym oddawaniem gazów i problemy urologiczne, z kolei chorzy z zachowanymi zwieraczami skarżą się na zaparcia. Pacjenci ze stomią znacznie częściej skarżą się na depresję, poczucie osamotnienia, a nawet rozpaczy z powodu złej samooceny i poczucia niekorzystnej zmiany wyglądu własnego ciała. Poczucie to występuje zwłaszcza u młodych pacjentów, częściej u kobiet. Rak jelita grubego wywołuje zaburzenia socjalne, takie jak niechęć do podjęcia aktywności zawodowej po operacji, ...

Streszczenie angielskie: The aim of this paper is to present the quality of life of patients operated on for rectal cancer. A comparative analysis of patients after abdominoperineal resection vs. patients after low anterior resection with preservation of sphincters is presented. When assessing the quality of life of patients, the following issues were considered; a) function of the whole organism and general condition (physical functioning); b) psychological well-being (psychological functioning); c) professional activity, relations with relatives and friends, leisure activities (social functioning); d) intimate relations (sexual functioning). In both groups of patients, both after abdominoperineal resection and after low anterior resection, a significant deterioration in the quality of life was noted. In spite of a good general health condition in the majority of cases (over 60 p.c. in both groups), frequent are irregular stools and diarrhoea. Stoma patients complain about uncontrolled passing of gas and urologic problems, while patients with preserved sphincter complain about constipation. Stoma patients significantly more often suffer depression loneliness and even despair due to low self-esteem and feeling of unfavourable change in body appearance. This feeling is present more often in younger patients and in women. Rectal cancer may cause social dysfunction, like reluctance to resume professional activity after surgery, limitation of socialcontacts, change of model of rest and leisure ...

249/2523

Tytuł oryginału: Current treatment of metastatic colorectal cancer.
Tytuł polski: Aktualne sposoby leczenia nawrotów raka jelita grubego.
Autorzy: Carrato Alfredo
Źródło: Proktologia 2002: 3 (2) s.175-178, tab., bibliogr. 17 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Uważa się, że u ok. 50 proc. pacjentów ze świeżo rozpoznanym rakiem jelita grubego (CRC) dojdzie do powstania przerzutów, a 5-letnia przeżywalność wynosi 50 - 60 proc. Dla poprawy przeżywalności pacjentów z zaawansowanym rakiem jelita grubego konieczne jest wdrożenie paliatywnej chemioterapii i leczenia skojarzonego. Dawniej podstawą leczenia CRC w fazie przerzutów był 5-fluorouracyl (5-FU), najpierw w monoterapii, a następnie w połączeniu z innymi cytostatykami wg odpowiednich schematów. Ostatnio zastosowano połączenie 5-FU z bardziej aktywnymi nowymi preparatami. Jednym z nich jest irinotekan (inhibitor topoizomerazy), który może indukować odpowiedź terapeutyczną nawet w przypadkach odpornych na 5-FU. Oksaliplatyna (związek platyny) w połączeniu z 5-FU wydłuża czas przeżycia bez progresji, jednak bez istotnego wydłużenia mediany całkowitego czasu przeżycia. Analiza genetyczna, mająca na celu określenie molekularnych czynników rokowniczych, powinna pomóc w zdefiniowaniu grup pacjentów, którzy najbardziej skorzystaliby z określonej metody leczenia. W osiągnięciu tego celu może pomóc zindywidualizowana chemioterapia, prowadzona w oparciu o indywidualne cechy konkretnego pacjenta. Połączenie chemioterapii i immunoterapii, zastosowanie inhibitorów metaloproteinaz, czynników blokujących angiogenezę, czynników blokujących transdukcję sygnałów i terapia genowa pozostają na razie we wczesnej fazie badań, ale stanowią logiczne kierunki dalszego rozwoju metod leczenia.

Streszczenie angielskie: 50 p.c. of newly diagnosed patients with colorectal cancer progress to metastatic disease with a 5-year survival of 50 - 60 p.c. There is need palliative chemotherapy in advanced CRS as well as adjuvant treatment to improve survival. In the past 5-fluorouracil has been the mainstream in metastatic CRC, initially as a monotherapy and then in conjunction with other agents in regimens. More recently 5-FU have been combined with active newer agents. One of them is irinotecan (topoisomerase inhibitor) which is capable of inducing response in-patients resistant to 5-FU. Oxaliplatin (platinum compound) in combination with 5-FU improved progression free survival without a significant increase in median survival time. Genetic analyses to help identify molecular prognostic factors should define more precisely which group of patients will benefit from a specific therapy. Customized chemotherapy based on each patient's individual features can help to attain thil goal. Combinations of chemotherapy with immunotherapy, metalloprotease inhibitors treatment, antiangiogenic agents, signal transduction inhibitors and gene therapy are early trials and are logical steps for future clinical development.

250/2523

Tytuł oryginału: Therapeutic approach of anal cancer.
Tytuł polski: Metody leczenia raka odbytu.
Autorzy: Karaitianos I. G., Maraghoudakis E. N.
Źródło: Proktologia 2002: 3 (2) s.179-182, tab., bibliogr. 20 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Rak odbytu występuje rzadko. Nazwa ta odnosi się do najczęstszego nowotworu złośliwego okolicy odbytu - raka kolczystokomórkowego. Jest to jedyny nowotwór złośliwy przewodu pokarmowego, gdzie możliwe jest wyleczenie bez radykalnej operacji. Rak odbytu jest w większości przypadków chorobą miejscową, przerzuty odległe w chwili rozpoznania stwierdza się u mniej niż 10 proc. pacjentów. Nowotwory odbytu klasyfikuje się tak jak nowotwory skóry, w oparciu o system TNM. W leczeniu dąży się do miejscowego i regionalnego opanowania nowotworu, w miarę możliwości przy zachowaniu funkcji zwieraczy. Dostępne metody leczenia obejmują: leczenie chirurgiczne, radioterapię i skojarzoną radio- i chemioterapię. Resekcja brzuszno-kroczowa, będąca postępowaniem z wyboru w przypadku raków zlokalizowanych poniżej linii grzebieniastej, w coraz większym stopniu jest zastępowana przez radioterapię, dającą taką samą przeżywalność. Radioterapia jako monoterapia daje 5-letnią przeżywalność u powyżej 70 proc. Jeszcze korzystniejsze jest połączenie radio- i chemioterapii. Schematy leczenia obejmują podanie 5-fluorouracylu z mitomycyną albo z cisplatyną. Miejscowe wycięcie niewielkich nowotworów z 1-cm marginesem, nawet z objęciem części aparatu zwieraczowego, pozwala uzyskać 5-letnią przeżywalność u powyżej 80 proc. Resekcja brzuszno-kroczowa jest stosowana w przypadku braku skuteczności chemioterapii i w przypadku wznowy miejscowej.

Streszczenie angielskie: Carcinoma of an anus is an uncommon disease, which refers to the commonest anal malignancy - squamous cell carcinoma. It remains the only carcinoma of GI tract curable without the need for definitive surgery. Anal cancer is predominantly a locoregional disease with fewer than 10 p.c. of patients having distant metastases at presentation. Tumors of anal margin are classified with skin tumors which is based on TNM stating. Treatment objectives include local and regional control over the tumor and maintenance of sphincter function when possible. The methods of treatment include surgery, radiotherapy and combined radio-chemotherapy. Abdominoperineal resection as a treatment of cancers located below dentate line is as a first intention treatment has been mostly replaced by radiotherapy with the same survival rate. Radiation treatment alone may lead to a 5-year survival rate in excess of 70 p.c. Combination of radio- and chemotherapy is even more beneficial. Treatment protocols involve administration of 5-fluorouracil with mitomycin or even better with cisplatine. Local excision of small carcinomas demonstrates over 80 p.c. 5-year survival rate obtained with 1 cm clear margin which may include part of sphincter apparatus. Abdominoperineal resection is reserved as salvage therapy fort he failure of prior chemotherapy and in local recurrences.

251/2523

Tytuł oryginału: Surgical achievements in Crohn's disease.
Tytuł polski: Wyniki leczenia chirurgicznego choroby Crohna.
Autorzy: Delaini G. G., Marinello P., Nifosi F., Carrara B.
Źródło: Proktologia 2002: 3 (2) s.183-184, bibliogr. 6 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Postęp w dziedzinie chirurgicznego leczenia choroby Crohna dotyczy głównie rozwoju technik umożliwiających oszczędzenie jelita, zmniejszenie urazu operacyjnego i zachowanie zwieracza odbytu. Plastyka miejsca zwężenia (strikturoplastyka) wskazana jest w przypadku zmian miejscowych, zwężeń mnogich na długich odcinkach jelita, u pacjentów po przebytych resekcjach, w przypadku nawrotu zwężenia w miejscu zespolenia krętniczo-okrężniczego oraz w przypadku zwężenia dwunastnicy. Strikturoplastyka pozwala uniknąć mnogich, rozległych resekcji i późniejszego wystąpienia "zespołu krótkiego jelita". Stosowane techniki obejmują metody Heineckego-Mikulicza, Finneya oraz izoperystaltyczną strikturoplastykę bok-do-boku. U niektórych pacjentów z chorobą Crohna możliwe jest wykonanie zespolenia zbiornika krętniczego z odbytem. Zabiegi laparoskopowe szybko zyskują popularność w chirurgicznym leczeniu choroby Crohna, a ich zalety to dobry wynik kosmetyczny, szybki powrót do zdrowia, krótki czas hospitalizacji, rzadkie występowanie powikłań i mniejsze ogólne koszty leczenia. Mniejszy jest też uraz operacyjny i mniejsze ryzyko powstania zrostów.

Streszczenie angielskie: New surgical achievements are especially related to bowel-sparing techniques, to reduction of surgical trauma and preservation of anal sphincter. Stricturoplasty is indicated in skin lesions, multiple strictures sited in long segments, in-patients with previous resection, recurrence of ileocolic anastomotic strictures, strictures of the duodenum. Stricturoplasty avaids multiple, broad resections and possible short bowel syndrome. Techniques employed include Heinecke-Mikulicz, Finney or side-to side isoperistaltic stricturoplasty. Ileal pouch anal anastomosis is feasible in selected patients with Crohn's disease. Laparoscopic procedures are diffusing rapidly in surgery for Crohn's disease, with good cosmetic results, faster recovery, shorter hospital stay, fewer complications and lower costs. Trauma is reduced and possibility to create adhesions is also reduced.

252/2523

Tytuł oryginału: Anal fistula.
Tytuł polski: Przetoka okołoodbytnicza.
Autorzy: Rosa G., Lolli P., Vicenzi L., Bottura D., Castellini C.
Źródło: Proktologia 2002: 3 (2) s.185-187, tab., bibliogr. 8 poz., streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Chirurgiczne leczenie przetok odbytu oparte jest na identyfikacji głównego kanału przetoki i jej wtórnych odgałęzień. Konfigurację przetoki można określić poprzez znalezienie jej ujść - zewnętrznego i wewnętrznego - oraz przebiegu samego kanału. Absolutnie przeciwwskazane jest nacinanie zwieracza odbytu, jeśli nie została jednoznacznie określona konfiguracja przetoki. Autorzy przeprowadzili 751 operacji przetoki odbytu. Wśród nich było 64 proc. przypadków wysokiego odejścia odgałęzień wtórnych, co znacznie utrudniało leczenie. U 85 proc. pacjentów kanał przetoki przechodził przez zwieracze. Najczęściej wykonywanym zabiegiem była fistulotomia i fistulektomia, jeśli przetoka nie obejmowała aparatu zwieraczowego. Przecięcie zwieracza było odraczane poprzez założenie setonu u 51 proc. spośród 436 przypadków przetok przezwieraczowych. Autorzy nie stosują techniki przesunięcia płata. Przed zabiegiem u wszystkich pacjentów przeprowadza się badania manometryczne w celu określenia zakresu nacięcia zwieracza. Po zabiegu odsetek nawrotów wynosił 4,2 p.c., a nietrzymanie stolca występowało u 0.98 proc. pacjentów.

Streszczenie angielskie: Surgical approach to anal fistulas has to be based on identification of main track and secondary extension. Track features come from identification of primary and secondary fistula opening and traject. It absolutely forbidden to cut sphincter when clear tract identification is lacking. Authors performed 751 fistula operations. Among them 64 p.c. had secondary high tracks, which makres the treatment more difficult. 85 p.c. patients had a fistula passingthough sphincters. The most performed operation was fistulatomy and fistulectomy if the fistula did not involve the sphincters. The sphincter cutting was delayed with seton in 51 p.c. of 436 transsphincteric fistulas. Authors do not practice advancement flap techniques. All patient are evaluated manometrically before the procedure to determine the extend of sphincter cut. Reported recurrence rate was 4.2 p.c. and incontinence in 0.98 p.c. of patients.

253/2523

Tytuł oryginału: Anual fissure - pathophysiology and management.
Tytuł polski: Szczelina odbytu - patofizjologia i postępowanie.
Autorzy: Pfeifer Johann
Źródło: Proktologia 2002: 3 (2) s.188-189, streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie polskie: Etiologia szczeliny odbytu pozostaje nieznana, jednak za najbardziej prawdopodobne przyjmuje się skojarzone działanie urazu, niedokrwienia i wady anatomicznej. W ostatnich dziesięcioleciach standardowym postępowaniem stała się sfinkterotomia boczne, z odsetkiem powikłań nie przekraczającym 5 proc. Leczenie zachowawcze polega na miejscowym stosowaniu toksyny botulinowej, blokerów kanału wapniowego i substancji uwalniających tlenek azotu. Zasada leczenia zachowawczego jest taka sama jak w przypadku leczenia chirurgicznego - zmniejszenie napięcia, ciśnienia i bólu w obrębie odbytu celem przyspieszenia gojenia. Sfinkterotomia chemiczna z zastosowaniem tlenku azotu stanowi korzystną alternatywę obarczoną niewielkim ryzykiem efektów niepożądanych i może być uznana za metodę z wyboru w leczeniu pacjentów ze szczeliną odbytu.

Streszczenie angielskie: Etiology of anal fissure is not known, however trauma with ischemia in combination with the anatomical structure is the most likely one. In the last decader standard therapy is lateral sphineterotomy with less than 5 p.c. of complication rates. Conservative management involves botulinum toxin, calcium channel blocker or NO-donor substances topical application. The idea behind the conservative treatment is identical to radical approach - to diminish anal pressure and pain in odrer to speed up the healing. Chemical sphincterotomywith NO is a viable option with small side effects and can be considered the procedure of choice for patients with anal fissures.

254/2523

Tytuł oryginału: Surgery for incontinence. Role of artificial sphincter.
Tytuł polski: Chirurgiczne leczenie nietrzymania stolca. Zastosowanie sztucznego zwieracza odbytu.
Autorzy: Pfeifer Johann
Źródło: Proktologia 2002: 3 (2) s.190-191, streszcz., rez.
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

ludzie

Streszczenie polskie: Uważa się, że ok. 1,5 proc. osób w populacji ogólnej cierpi z powodu nietrzymania stolca, a brudzenie bielizny występuje u ok. 5 proc. U pacjentów z nasilonym i nieodwracalnym nietrzymaniem stolca możliwości leczenia chirurgicznego są ograniczone. Poza operacjami polegającymi na przemieszczeniu mięśni duże nadzieje wiąże się z implantacjami sztucznego zwieracza (artifical bowel sphincter, ABS). Jest to urządzenie zbudowane z mocnego elastomeru silikonowego, wypełnione płynem i składające się z mankietu, pompy i balonu regulującego ciśnienie. Odsetek powikłań jest niewielki, a głównym problemem są zakażenia. Chirurgiczne leczenie nietrzymania stolca musi być zindywidualizowane i dostosowane do konkretnej sytuacji klinicznej. Duże nadzieje budzi zastosowanie systemu ABS, pod warunkiem, że uda się uniknąć zakażeń.

Streszczenie angielskie: It is said that up to 1.5 p.c. of population suffer from fecal incontinence, with soiling rate up to 5 p.c. For patients with severe irreparable fecal incontinence the surgical options are limited. Besides muscle transfer operations implantation of artificial bowel sphincter is a promising technique. It consist of fluid-filled, solid silicone elastomer device built of: cuff, pump and pressure regulating ballon. The complication rates seem to be very low with predominant factor of infection. Surgical treatment of fecal incontinence must be tailored to individual patient although ABS system is a promising device if infection can be avoided.

255/2523

Tytuł oryginału: 7th Central Congress of Coloproctology and Viscerosynthesis Italian - Baltic Week, June 2-4, 2002, Kaunas, Lithuania. Abstracts, [programme].
Źródło: Proktologia 2002: 3 (2) s.192-223, tab. - 7 Środkowoeuropejski Kongres Koloproktologii i Wiscerosyntezy - tydzień włosko - bałtycki Kaunas 02-04.06. 2002
Sygnatura GBL: 313,572

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

256/2523

Tytuł oryginału: Implication of immunological abnormalities after adenotonsillotomy.
Autorzy: Zielnik-Jurkiewicz Beata, Jurkiewicz Dariusz
Źródło: Int. J. Pediatr. Otorhinolaryngol. 2002: 64 (2) s.127-132, tab., bibliogr. 20 poz.
Sygnatura GBL: 306,189

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The adenoids and tonsils are thought to be essential parts of the system protecting organism against pathogens invading the upper respiratory tracts. Human adenoids and tonsils are known to be immunologically reactive lymphoid organs, which manifest specific antibodies and B and T cells activity in response to variety of antigens carrying out the functions of humoral and cellular immunity. The purpose of the study was to observe the changes in systemic immunity in children with hypertrophy of adenoids and tonsils treated in Department of Laryngology, Children's Hospital in Warsaw in period 1994-1999. The study comprised 80 patients (33 girls and 47 boys, aged from 3 to 14 years, mean age 6.8 years) with diagnosed hypertropphy of adenoids and tonsils. The diagnosis of hypertrophy of adenoids and tonsils was based on characteristic history and laryngological examination. All patients were scheduled for adenotonsillotomy. The control group comprised 40 people (14 girls and 26 boys, aged from 3 to 15 years, mean age 7.6 years) without hstory of the recurrent upper tract infections. In all patients we carried out following examinations: serum levels of immunoglobulins A, G, M (humoral immunity); percentage of T lymphocytes (CD3); percentage of T helper (CD4) and T cytotoxic (CD8) lymphocytes (cellular immunity) and delayed cutaneous hypersensitivity - Multitest CMI (cell mediated immunity). Our study demonstrate that in children with hypertrophy of adenoids and tonsils exist changes in the immunological parameters...

257/2523

Tytuł oryginału: Raccoon dog (Nyctereutes procyonoides) - the new host of Echinococcus multilocularis in Poland.
Autorzy: Machnicka-Rowińska Barbara, Rocki Bogusław, Dziemian Ewa, Kołodziej-Sobocińska Marta
Źródło: Wiad. Parazytol. 2002: 48 (1) s.65-68, tab., bibliogr. [14] poz.
Sygnatura GBL: 303,658

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The alimentary canals of 9 species of domesticated or wild animals were examined for the presence of E. multilocularis. The tapeworm was found in 9 red foxes (Vulpes vulpes) out of 155 examined (5.8 p.c.) and in 2 raccoon dogs (Nyctereutes procyonoides) of 25 examined (8.0 p.c.). Our studies proved that in Poland the raccoon dog can also be infected with E. multilocularis.

258/2523

Tytuł oryginału: Early toxicity of combined treatment in localised and locally advanced prostate cancer: hormone therapy with conformal radiotherapy.
Autorzy: Milecki Piotr, Stryczyńska Grażyna, Stachowski Tomasz, Kwias Zbigniew
Źródło: Urol. Pol. 2002: 55 (2) s.48-52, bibliogr. 28 poz., streszcz.
Sygnatura GBL: 304,054

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: Cel. Celem pracy była ocena wczesnej toksyczności skojarzonego leczenia (hormonoterapi + radioterapia konformalna 3D [3 d-CRT) u chorych z rakiem gruczołu krokowego. Materiał i metody. W okresie od kwietna 1999 roku do grudnia 2000 roku w Wielkopolskim Centrum Onkologii w Poznaniu 42 chorych z rakiem gruczołu krokowego (T1-T3N0M0) zostało leczeniu poddanych radioterapią konformalną 3D w skojarzeniu z hormonoterapią. Analizowana grupa chorych obejmowała następujące stopnie zaawansowania klinicznego: T1 = 4 chorych, T2 = 11 chorych, T3 = 27 chorych. U żadnego z poddanych analizie nie stwierdzono obecności przerzutów odległych. U wszystkich chorych przed rozpoczęciem leczenia napromienianiem, oraz w jego trakcie, zastosowano leczenie hormonalne, składające się z agonisty LHRH lub obustronnej orchidektomii w skojarzeniu z flutamidem. Leczenie napromienianiem prowadzono przy zastosowaniu fotonów 15 MV w dziennej dawce 1,8 Gy do średniej dawki całkowitej 71,7 Gy (67,8 - 72 Gy). Ostry odczyn popromienny był oceniany według klasyfikacji zaproponowanej przez Radiation Therapy Oncology Group (RTOG). Wyniki. Wszyscy chorzy ukończyli cały cykl napromieniania bez konieczności wprowadzania dodatkowych przerw z powodu nasilenia objawów ubocznych terapii. Do najczęstszych objawów ubocznych prowadzonego leczenia hormonalnego w analizowanej grupie zaliczono: "uderzenia gorąca", poty, powiększenie gruczołów piersiowych, uczucie dyskomfortu w jamie brzusznej. Wszystkie powyższe ...

Streszczenie angielskie: Purpose: to evaluate acute toxicity of combined treatment (androgen ablation and 3D conformal radiotherapy [3D-CRT] in patients with localised and locally advanced cancer of the prostate. Materials and methods. Between April 1999 and December 2000, at the Wielkopolskie Cancer Centre in Poznan, 42 patients with prostate cancer (T1-T3 N0 M0) were treated with 3 D conformal radiation therapy and hormone therapy. Patients representeda localised disease (1 = 4 patients, T2 = 11 patients) and locally advanced disease (T3 = 27 patients). No patients had clinically detectable distant metastases. Neo-adjuvant androgen ablation therapy (LH-RH agonists or bilaeral orchidectomy and flutamide) was given to all patients. Radiotherapy was performed using 15 MV photons in the daily fraction of 1.8 Gy to the total median dose of 71.7 Gy (range, 67.8 to 72 Gy). Acute toxicities were evaluted according to the Radiation Therapy Oncology Group (RTOG) morbidity scoring scale. Results. All patients completed the entire course of radiotherapy and were assessable for evaluation of acute toxicities. The most common side effects of androgen ablation were "hot flushes", gynaecomastia, and slight discomfort in the abdomen, although these were of mild intensity. The main problems during irradiation and for a few weeks after the completion of radiotherapy were related to: - genito-urinary tract (rgency, nocturia, dysuria). Toxicities were as follow: grade 0 and 1: 80 p.c. of patients and grade 2: ...

259/2523

Tytuł oryginału: Expression of mRNA for cytokines (TNF-ŕ and IL-1ŕ) in human cytomegalovirus (HCMV) and hepatitis B virus (HBV) infections.
Autorzy: Kasprzak A[ldona], Zabel M[aciej], Wysocki J., Seidel Surdyk-Zasada [Jolanta] J[oanna], Filipiak B[arbara]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.63-68, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

dzieci 6-12 r.ż.

Streszczenie angielskie: The study was aimed at detecting cellular sources of transcripts for two cytokines, TNF-ŕ and IL-1ŕ in infection with human cytomegalovirus (HCMV) or hepatitis B virus (HBV). The studies were performed on paraffin sections of organs (liver, pancreas, spleen, lungs) obtained upon autopsy from a child decreased due to acute inborn HCMV infectio on paraffin secions of liver biopsy, obtained from a child with HCMV-induced chronic hepatitis, and of liver biopsies obtained from children with chronic type B hepatitis (n = 13). The classical in situ hybridization was applied with digoxygenin-labeled probes and amplification by the ImmunoMax technique. In HCMV infection, the most pronounced expression of mRNA for TNF-ŕ and II-1ŕ was detected in pancreatic islets (mainly in á cells) and, then, in a decreasing sequence, in liver (in macrophages and sinusoidal endothelial cells) and in lungs (in alveolar macrophages). No expression of the two cytokines was detected in the sinusoidal endothelial cells) and in lungs (in alveolar macrophages). No expression of the two cytokines was detected in the spleen. In HBV infection, weak expression of TNF-ŕ in the liver were observed, mainly in sinusoidal endothelial cells and in mcrophages as well as in hepatocytes. These results were confirmed by immunocytochemical experiments.

260/2523

Tytuł oryginału: Ontogeny of the expression of some catecholamine synthesising enzymes in the female porcine preoptic area.
Autorzy: Sienkiewicz Waldemar
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.69-76, il., tab., bibliogr. 60 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zarodki

Świnie

Streszczenie angielskie: Ontogeny of the catecholaminergic system of the preoptic area (PA) was studied in various animal species including mice, rats, cats and lower vertebrates. Until now, there has been no data about development of catecholaminergic structures in the porcine PA. To study this problem, hypothalami from six groups of animals were collected. Three groups of foetuses (70, 84 and 112 days old) and three groups of female pigs (1 day, 10 weeks and 7 - 8 months old) were used. Nerve structures immunoreactive for the studied substances: tyrosine hydroxylase (TH), dopamine á-hydroxylase (DáH) and phenylethanoloamine-N-metylthransfrease (PNMT) were observed in different periods. In PA, TH-IR (immunoreactive) structures appeared before 70th day of foetal life, DáH-IR between 70th and 84th day of foetal live and PNMT-IR only in 10-week old and adult animals. In the PA of 70-day old foetuses, single smooth and varicose nerve fibres immunoreactive only to TH were found. In PA of 84-day old foetuses, additionally, single nerve cell bodies immunoreactive to TH were shown and some of them also contained immunoreactivity to DáH. In Pa of 1-day old piglets, moderate numbers of nerve fibres immunoreactive to TH and only single TH/DáH-IR nerve terminals were observed. TH-IR nerve cell bodies were also moderate in number and many of them contained simultaneously immunoreactivity to DáH. In PA of 10-week old pigs, a moderate number of immunopositive nerve fibres was observed. They contained mainly TH, but part of them stained also for TH/DáH. Only very few nerve fibres containing exclusively DáH were found...

261/2523

Tytuł oryginału: Proceedings of the conference "Flow cytometry and quantitative microscopy in medical diagnostics", May 21-22, 2001, Poznań, Poland.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.99-116, il., tab., bibliogr. [przy artyk.] - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

262/2523

Tytuł oryginału: Decreased density of the CCR5 receptor on the surface of CD4+ lymphocytes and monocytes/macrophages is associated with the CCR5-59653T transition in the promoter region.
Autorzy: Lewandowska M., Jagodziński P. P., Trzeciak W[iesław] H.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.101-102, il., bibliogr. 7 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We compared the denistity of the CCRS receptor on the surface of CD4+ lymphocytes and monocytes/macrophages ot the homozygote (CCR5-59653C) and the heterozygote (CCR5-59653T), bearing CCR-2-64V alleles. Flow cytometric analysis revealed lower density of the CCR5 receptor on the surface of CD4+ lymphocytes and monocytes/macrophages of the heterozygote than in the same cells of the homozygote. Our observation might explain slower replication of HIV and the delay in progression to AIDS in the individuals beraing CCR5-59653T transition.

263/2523

Tytuł oryginału: Reactivity of alveolar macrophages in lung cancer patients and healthy subjects: surface ICAM-1 after INF-ç stimulation in vitro.
Autorzy: Hoser G., Grubek-Jaworska H., Droszcz P., Domagała-Kulawik J., Dąbrowska M., Chazan R.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.103-104, il., tab., bibliogr. 6 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The linearity of ICAM-1 expression on alveolar macrophages (AM) before and after INF-ç stimulation in healthy and lung cancaer subjects were compared. AM were collected by bronchoalveolar lavage and incubated with/without INF-ç according to standard procedures. The harvested calls were analyzed by flow cytometry using mooclonal antibodies against leucocytes and macrophages. Only viable cells were analyzed. Stimulation with INF-ç revealed two AM subpopulations of similar size differentiated in the intensity of ICAM-1 expression. They were not distinctly marked in every studied case. our preliminary results did not confirm the previously reported decreasing reactivity of AMs after INF-ç stimulation in lung cancer patients.

264/2523

Tytuł oryginału: DNA ploidy assessment method applied to primary breast carcinoma FNA biopsies.
Autorzy: Czuba A., Lange D., Chmielik E., Wołoszyńska K., Wojciechowska E., Śnietura M., Liszka J.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.105-106, il., tab., bibliogr. 5 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The goal of our study was to assess suitability of FNA biopsy material as a source of samples (cell suspension) for DNA ploidy assessment in neoplastic tumors using flow cytometry. DNA ploidy is an established prognostic factor in many types of cancers. Aneuploid breast tumors are characterized by increased aggressiveness which manifests itself through rapid local progression and metastatic spread. Investigated specimens were breast cancer FNA biopsy cell susupensions. Measurements were performed using flow cytometry. Material studied comprised 143 cases analyzed in 1999-2000. We found in this group 101 carinoma cases with aneuploid typ and 42 cases of primary breast carcinoma with diploid type of cell cycle. Immunocytochemical assessment of estrogen receptor and progesterone receptor status was performed in group of 105 cases DNA ploidy was compared to receptor status of the invetigated cells. DNA aneuploidy correlated wtih weak or no reaction for the presence of estrogen and progesterone receptors. Our study demonstrates the suitability of DNA ploidy assessment method applied to cytological material from FNA biopsies.

265/2523

Tytuł oryginału: Fluorescence in situ hybridization (FISH) - application in research and diagnostics.
Autorzy: Budny B., Kanik M., Latos-Bieleńska A[nna]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.107-108, il., bibliogr 10 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The methods of molecular cytogenetics, in particular fluorescence in situ hybridization (FISH), are widely applied in cytogenetics for identification of numerical and structural chromosomal abnormalities, which are difficult to detect by routine cytogenetic techniques. Due to many advantages, FISH is used in research (gene mapping, gene expression studies, interspecies chromosome homology), and clinical diagnostics (chromosomal aberrations analysis in pre- and postnatal diagnostics, onclology). The techniques of in situ hybridization (ISH) are often employed in addition to classical banding techniques, in case where binding pattern is not reliable. This paper focusts on particular clinical examples, wher FISh was successfully used to identify structural and numerical chromosomal aberrations.

266/2523

Tytuł oryginału: The influence of photodynamic reaction on R2C cells.
Autorzy: Wołuń-Cholewa M[aria], Warchoł J[erzy] B.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.109-110, il., bibliogr. 10 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Photodynamic therapy (PDT) represents a theapeutic approach in which photosensitised neoplastic cells undergo destruction under effect of light. In this study we have attempted to define effects of photochemotherapy R2C cells, sensitised with protoporphyrin IX (PpIX) and to find out whethe inhibition of gene expression by cycloheximide affects development of lesions in the cells. The photosensitised necrotic lesions was followed in the cells, using the fluorescent staining with propidium iodide and Hoechst 33342. The expresiments demonstrated that PpIX and light, acting in parallel, induce development of apoptotic and necrotic lesions in R2C cells. Intensity of the lesions correlated with concentration of the applied photosensitiser and with duration of light exposure. Using cycloheximide, we also inhibited protein expression in cells photosensitised with protoporphyrin before they were exposed to light. In the latter case, development of apoptosis was clearly intensified which might be explained by inhibition of antiapoptotic protein synthesis in the cells.

267/2523

Tytuł oryginału: Plasma membrane translocation of phosphatidylserine in human spermatozoa.
Autorzy: Kotwicka M[ałgorzata], Jendraszak M., Warchoł J[erzy] B.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.111-112, il., bibliogr. 11 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The purpose of the study was to examine the phosphatidylserine translocation in human spermatozoa membrane during capacitation. Material consisted of human semen from normozoospermc men. Spermatozoa were stained with fluorescein-labelled annexin V. The presence and distribution of annexin V binding sites were analysed using the fluorescence micoroscope. Within first 60 min after ejaculation, 5 - 39 proc. viable annexin V-positive spermatozoa were detected. The annexin V binding sites were found mainl in the midpiece. After 4 to 8 h of incubation of spermatozoa in capacitation medium (BMI), the number of cells positively stained with annexin V increased. After capacitation, the localisations of phosphatidylserine was changed and the annexin V binding sites were found also in the acrosomal region but never in the equatorial area. The process of the phosphatidylserine translocation observed during our experiments may reflect changes of the plasma membrane occurring during capacitation or, less likely, apoptosis of spermatozoa.

268/2523

Tytuł oryginału: The influence of storage time upon the real thickness of the histological brain sections.
Autorzy: Dziewiątkowski J[erzy], Spodnik J[anusz] H., Wójcik S., Spodnik E., Moryś J[anusz]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.113-114, il., bibliogr. 15 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The aim of the study was to assess the effect of storage time on the actual thickness of the histological sectons. The study was performed on 5 brains of adult Wistar rats. The most rapid changes occur at the beginning of the storage process, after about one month the dynamics of changes decreases, but still the thickness of the sections diminishes. It is suggested that quantitative analyses should be performed in similar period of time and the critical care should be taken not only of the chemicals and procedures used, but also of the control of environmental factors.

269/2523

Tytuł oryginału: Relation between the age of specimen and the shrinkage of brain frozen sections.
Autorzy: Dziewiątkowski J[erzy], Wójcik S., Spodnik J[anusz] H., Spodnik E., Kowiański P[rzemysław, Moryś J[anusz]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.115-116, il., bibliogr. 13 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: The aim of the study was to assess the effect of age of the animal upon the real thickness of the frozen sections. The study was performed on 19 rabbit brains. The thickness of the frozen sections regardless of their staining is age-dependent. The relation is proportional during the period from 7 to 180 postnatal day and characterizes both immunohistochemical as well as cresyl violet-stained sections; moreover, changes of the section thickness proceed parallely. It is suggeted that especially for some stereological parameters all required procedures should be standardized to achieve comparable and unbiasedly itnerprtable results.

270/2523

Tytuł oryginału: Proceedings of the 37th Symposium of the Polish Histochemical and Cytochemical Society, September 2-5, 2001, Cracow, Poland.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.117-234, il., tab., bibliogr. [przy artyk.] - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Temat korporatywny:

Polskie Towarzystwo Histochemików i Cytochemików

271/2523

Tytuł oryginału: Advances in immunocytochemistry.
Autorzy: Noorden Susan Van
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.121-124, il., bibliogr. 20 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Recent advances in immunohisto/cytochemical methods have been directed towards new fluorescent labaels, and to increasing sensitivity and improving methods for multiple immunostaining. The newestfluorescent dyes come in many colours, are much mosre stable than fluorescein isothiocyanate and give brighter fluorescence. The greatest impact on sensitivity comes from heat-induced antigen retreival on paraffin sections. Biotinylasted tyramide can also increase enormously the amount of label on a preparation. The immunogold method with silver enhancement has been improved by nanogold, which is smaller than colloidal gold, attracts more silfer and increases the sensitivity even further. The difficulties of multiple labelling with two or more primary antibodies raised in the same species due to cross-binding between reagents have largely been overcome. There are new methods of blocking spare reactive sites on the first reaction by strong heat, e. g. from microwaving in buffer. Alternatively, one of the antibodis can be directrly biotinylated very easily with a new commercially available kit.

272/2523

Tytuł oryginału: Comparison of tchniques permitting to detect apoptosis in situ.
Autorzy: Drąg-Zalesińska M[ałgorzata], Wysocka T[eresa], Dumańska M[ałgorzata], Jagoda E[wa], Zabel M[aciej]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.125-126, il., tab., bibliogr. 4 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Several morphological and biochemical techniques are inuse for identification of apoptotic and necrotic cells ina studied cell population. It is essential to define not only the type of cell death but also to identify the apoptotic process itself, which represents a multistage, active processes, requiring activation of a molecular event cascade. In the present study, we have examined and discussed effectiveness of the selected techniques detecting apoptosis in hymphocytes exposed to incubation at an elevated temperature. The appraisal involved detection of caspase-3 active form, detection of Bel-2, TUnel reaction, the comet assay and electrophoresis of DNA.

273/2523

Tytuł oryginału: Folia Histochemica et Cytobiologica
Tytuł angielski: Correlation of histopathological and biochemical appraisal of anthracyclin-induced myocardium damage.
Autorzy: Dzięgiel P[iotr], Surowiak P., Zabel M[aciej]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.127-128, il., bibliogr. 8 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Treatment of various tumor types with anthracyclin cytostatic drugs (DNR - daunorubicin and DOX - coxorubicin) is limited by their highe cardiotoxicity. This study was aimed at examining effectiveness of histopathological appraisal of myocardial cell injury induced by the cytostatic drugs and evaluated according to Bilingham and by MTS methods as compared to results of parallel biochemical assessment of lipid peroxidation indices (MDA - malonyldialdehyde, 4-HDA - 4-hydroxyalkenes). The experiments were performed on rats intoxicated with DNR or DOX in an acute manner (1 x 10 mg/kg body weight, i.v.) or a subchronic manner (3 x 3 mg/kg body weight i.v.). Significant positive correlations were demonstrated between results of histological and biochemical appraisal in rats intoxicated in the acute manner with DNR (r = 0.91), intoxicated in the subchronic manner with DNR (r = 0.90) and in rats intoxicated in the acute or subchronic manner with DOX (r = 0.91, r = 0.77, respectively). The obtained results have confirmed the free radical mechanism of cardiomyocyte injury induced by anthracyclines and the applied techniques of evaluating the destruction may be used independently of each other.

274/2523

Tytuł oryginału: Value of PCR technique in detection of Helicobacter pylori in paraffin-embedded material.
Autorzy: Ciesielska U[rszula], Jagoda E[wa], Marciniak Z[ofia]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.129-130, il., tab., bibliogr. 6 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Methods which permit effective detection of Helicobacter pylori are based on endoscopic sampling of gastric mucosa followed by a direct or indirect demonstration of Helicobacter pylori bacilli, e.g. searching for specific proteins or gene fragments. PCR technique represents one ot techniques employed for diagnosing the biopsies. The present study was aimed at examining how effective is the detection of Helicobacter pylori in paraffin-embedded material by PCR technique using primers for the Ure C gene fragment. The material for studies involved gastric biopsies, routinely fixed in formalin and embedded in paraffin. In order to perfirm PCR, DNA was isolated and purified, subjected to amplification with the use of starters for the Ure C gene fragment. The performed experiments have confirmed suitability of PCR technique for detection of Helicobacter pylori.

275/2523

Tytuł oryginału: The dynamics of morphological changes in the pyloric endocrine cells of rats with uremia.
Autorzy: Kasacka I[rena], Azzadin A[rsalen], Malla H.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.177-178, il., bibliogr. 14 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Disturbances in renal homeostatic function lead to changes in endocrine cell secretory activity. The aim of this study was the histomorphological estimation of dependence of gastric APUD system cell morphology and function on the time after subtotal nephrectomy in Wistar rats. Fragments of gastric pylorus were collected 1, 2, 4, and 6 weeks after nephrectomy. Paraffin sections were stained with H+E and by silver impregnation. Immunohistochemical reactions with the use of specific antibodies against calcitonin gene-related peptide (CGRP), synaptophysin (SPh), somatostatin (ST), and neuron-specific enolase (NSE) were also performed. Immunoreactivity of the examined substances in the pyloric mucosa in the first week after nephrectomy was lowr than in the control group. However, in the following time intervals, endocrine cells showd stronger immunostaining in comparison with the control rats. The results suggest that chronic renal failure can modulate secretory activity of APUD system cells.

276/2523

Tytuł oryginału: Sequential translocation of tyrosine kinases Lyn and Syk to the activated Fcç receptors during phagocytosis.
Autorzy: Strzelecka-Kiliszek A., Sobota A.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.131-132, il., bibliogr. 13 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Tyrosine phosphorylation of numerous proteins is one of the earliest events detectable during Fcç receptor-mediated phagocytosis. We demonstrate that IgG-coated particles associated with the surface of macrophages are enriched with numerous rytosine-phosphorylated proteins. During particle internalization the proteins are still associated with particles but their phosphorylation is reduced. Lyn kinase is phosphorylated both at particle binding and internalization steps. The phosphorylated Syk kinase is the major kinase associated with engulfed particles. Immunofluorescent studies confirm spatial and temporal distribution of Lyn and Syk kinases at different stages of phagocytosis. Our data indicate that ligation of Fcç receptors activites Lyn followed by Syk kinase and in the results multimolecular complex of the kinases and several accompanying tyrosine phosphorylated proteins with Fcç receptors is organized leading to local reorganization of actin-based skeleton and particle uptake.

277/2523

Tytuł oryginału: Efficiency of lipofection of adherent cells is limited by apoptosis.
Autorzy: Bednarek I., Czajka M., Wilczok T.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.133-134, il., bibliogr. 10 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Stability of gene expression and transffection efficiency plays the main role in the application of gene transfere method. In somatic cell gene delivery, expression of the gene product is limite by the function of the cell to which it is delivered. In te present study analyzing the lipofected adherent cells, we have shown that lower level of transgene: á-galctosidase activite at later time period correlated with decrease in cell viability, which was shown to be due to apoptosis. Apoptosis following DNA uptake occurred only when DNA was present during lipofection.

278/2523

Tytuł oryginału: Effects of the combination of a proteasome inhibitor (PSI) and an inhibitor of ubiquitin-ligases (Leu-Ala) on the ultrastructure of human leukemic U937 cells.
Autorzy: Biały Ł. P., Ziemba H[alina], Pleban E., Wójcik C[ezary]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.135-136, il., bibliogr. 14 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We have used the dipeptide Leu-Ala in an attempt to prevent the formation of ubiquitin-protein conjugates in U937 cells by inhibition of cellular E3 enzymes (ubiquitin ligases). Proteasome inhibitors induce the formation of perinuclear of ubiquitinated proteins and proteasomes (aggresomes) in the area of the proteolytic center of the cell. Leu-Ala did not prevent the formation of those aggregates under the action of PSI (peptidyl aldehyde, selective inhibitor of the chyotrypsin-like activity of teh proteasome), however it induced an accumulation of lipid droplets in treated cells, suggesting a previously unknown involvement of Leu-Ala in lipid metabolism. We conclude, that either Leu-Ala is not able to completely inhibit the cellular E3 enzymes or some of those enzymes are insensitive to this dipeptide, allowing therefore the build-up of ubiquitin-conjugates in the proteolytic centre of the cell.

279/2523

Tytuł oryginału: The effect of quercetin on the expression of heat shock proteins and apoptosis induction in monkey kidney cell line GMK.
Autorzy: Jakubowicz-Gil J., Rzymowska J., Paduch R., Gawron A.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.137-138, il., tab., bibliogr. 10 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

małpy

in vitro

Streszczenie angielskie: The present study was designed to investigate whether quercetin, a very common flavonoid widely distributed in many plants, can induce apoptosis in monkey kidney cells (GMK). Involvement of the expression of heat shock proteins Hsp72, Hsp3, Hsp27 and the significance of cell culture model in this process were also examined. The studies have shown that quercetin alone and in combination with the heat shock can induce apoptosis and necrosis in vitro in the sutided cells, but the percentagae of affected cells did not exceed 3.9 p.c. In the same experimental conditions, the expression of Hsp73, Hsp72 and Hsp27 increased in cells cultured in two-dimensional systm and decreased in three-dimensional model. This indicates that strong inhibition of heat shock proteins in GMK cells is not correlated with an adequate increase in the sensitivity of cells to undergo apoptosis. It also shows that the sensitivity of cells, manifested by Hsp expression and apoptosis induction, depends on the culture model and culture conditions.

280/2523

Tytuł oryginału: The expression of PDGF, its receptors and capillary morphometry in the developing lung: quantitative studies.
Autorzy: Marszałek A[ndrzej], Biczysko W[iesława], Wąsowicz M[arcin], Daa T., Yokoyama S.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.139-140, il., bibliogr. 11 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zarodki

Streszczenie angielskie: The aim of the present study was the evaluation of the expression levels of PDGF-A and B, and PDGFR-ŕ, and changes of apillary cross-sectional areas during terminal vascular network formation in the developing lung. Pathogen-free fetuses and newborn Wistar rats were used. Lungs were processed for frozen sections used for immunohistochemical staining and parallel tissue specimens were prepared for transmission electron microscopy. The expression levels of PDGF-A and B, and PDGRR-ŕ were obtained using quantitative image anlysis. Additionally, the morphometry of capillary cross-sectional area was performed. We found that the expression levels of the studied antigens fluctuated, and the highest values were found in 2-day old fetuses (p 0.0001). Although there were no significant differences of the area of "a single" capillary, the area of the endothelium decreased significantly in perinatal period (p 0.0001). We conclude that the statistically significant changes of the studied antigens' expression as well as the of capillary cross-sectional area occur in the perinatal period.

281/2523

Tytuł oryginału: A search for colocalization of mGlu1a receptors with CRF or NPY in the rat brain amygdala.
Autorzy: Śmiałowska M., Wierońska J. M., Wędzony K.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.153-154, il., bibliogr. 11 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Excitatory neurotransmitter glutamate, as well as corticoliberin (CRF) and neuropeptide Y (NPY) play an important roel in fear and anxiety. Among the brain structures engaged in these effects the important one is amygdala. In the present study, a single and double immunohistochemical staining techniques were used in order to visualize CRF, NPY and metabotropic glutamate receptors (mGluR1a) in rat amygdala. MGluR1a belongs to class of postsynaptic excitatory receptors and has a preferable somatic localization. CRF and NPY were localized using rababit polyclonal antibodies, and mGluR1a using a mouse monoclonal one. Then, ABC-peroxidase and DAB or benzidine were used. Upon single immunostaining, NPY and CRF were found in some nerve cell bodies and fibres in the amygdala. The immunoreactivity of mGluR1a was observed in some nerve cells, processes and fibres, especially on the border between the central and the basolateral nuclei and ventrally to that region. Double staining revealed mGlR1a-IR on some CRF- and NPY-immunoreactive nerve cell bodies and processes. The obtained results indicate that mGlu1a receptors may control at least some NPY and CRF neurons in the amygdala.

282/2523

Tytuł oryginału: The influence of interleukin-1 and interleukin-6 on expression of proteins involved in steroidogenesis in Leydig cells and on their apoptosis or necrosis in vitro.
Autorzy: Sawiński P[iotr], Łukaszyk A[ndrzej]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.159-160, il., tab., bibliogr. 8 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: This paper presents a study on the expression of proteins involved in steroidogenesis in rat Leydig cells: StAR protein and aromatase, in relation to the age of animals and to the influence of interleukin-1 and interleukin-6. In addition, their effect on apoptotis and damage of Leydig cells was investigated. Leydig cells were isolated rom rats at certain age intervals and exposed to different concentrations of the above-mentioned cytokines. Next, total RNA was isolated from Leydig cells and RT-PCR with primers specific for rat StAR protein and aromatase was performed. The products were analysed by agarose gel electrophoresis. Leydig cells were also stained with propidium iodide and Hoechst 33342 to estimate the percentage of apoptotic or necrotic or necrotic cells.The results seem to confirm the role of interleukins in the control of expression of enzymes and regulatory factors involved in steroidogenesis in rat Leydig cells, and in the regulation of their apoptosis and necrosis. The effect of the studied interleukins on Leydig cells is exerted during development and after adolescence.

283/2523

Tytuł oryginału: Immunohistochemical localization of androgen receptors in testicular cells of mice with mosaic mutation.
Autorzy: Kowal M., Kotula-Balak M., Styrna J., Bilińska B.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.161-162, il., bibliogr. 16 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Mice with mosaic mutation could be one of the models of human Menkes disease, which is associated with abnormal cooper metaolism. The aim of the present study was to localize androgen receptors (ARs) in the testes by means of immunohistochemistry. AR expression was observed in the nuclei of all somatic cells such as Leydig cells. Sertoli cells, and peitubular cells in sections from testes of control and mosaic mutant males. In the latter, very strong immunoreactivity for ARas well as higher levels of steroid hormones in homogenates were noticed in comparison to control mice. No positive immuoreaction for ARs was seen in control sections incubated without the primary antibody.

284/2523

Tytuł oryginału: Germ cells with nuclear DNA fragmentation related to apoptotic cells in rat testis in experimental hyperprolactinemia induced by metoclopramide.
Autorzy: Laszczyńska M[aria], Słuczanowska-Głąbowska S[ylwia], Piasecka M[ałgorzata], Skowron J[oanna], Dębińska-Szymańska T.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.163-164, il., tab., bibliogr. 9 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The cells with nuclear DNA fragmentation related to apoptosis were detected by TUNEL technique in the seminiferous epithelium of control rats and of rats with experimental hyperprolactinemia induced by metoclopramide. The percentage of convoluted tubules with apoptotic cells and the number of apoptotic cells (predominantly spermatogonia and spermatocytes) was increased in the experimental group. The results indicated stage-specific germ cell apoptosis. In the experimental group, apoptotic cells were most evident at early (I-IX), middle (VII-VIII) and late (XII-XIV) stages of teh seminiferous epithelium cycle, as revealed by light and electron microscopy. We suggest that a decreased concentration of testosterone and an increased concentration of prolactin could disturb spermatogenesis and contribute to the intensive apoptosis of germ cell in rats with hyperprolactinemia. sertoli cells which have receptors for testosterone and prolactin and play an important role in spermatogenesis and in the initiation of apoptosis in seminiferous epithelium, could mediate such an influence of both hormones.

285/2523

Tytuł oryginału: New method of quantitative analysis of seminiferous epithelium in male guinea pigs.
Autorzy: Surmacki P[iotr], Panasiuk J.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.165-166, tab., bibliogr. 3 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnki morskie

płeć męska

Streszczenie angielskie: The aim of the study was the comparison of a newly developed method of quantitative analysis of the seminiferous epithelium (S) cells with the traditional method based on stage classification according to Clermont. In 4000 sections of seminiferous tubules the following parameters have been evaluated: in procedure I - the frequency of cells at the particular stages of the SE cycle, in procedure II - the frequency of particular cell types forming SE. In the two procedures, 17726 and 14670 cells were assessed, respecatively. In both procedures, the mean values of the studied cells per animal (I - 886.3 ń 15.4; II - 733.5 ń 93.9) and the distribution of the particular types of cells were similar. The new method of quantitative analysis is less laborious and cheaper. It may be applied instead of the method used so far.

286/2523

Tytuł oryginału: Temperature dependent hysteresis-like changes of motility parameters in hyman spermatozoa.
Autorzy: Warchoł W[ojciech], Kotwicka M[ałgorzata]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.167-168, il., bibliogr. 5 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: We studied the imapct of temperture changes on selected parameters of normozoospermic spermatozoa motility. The examinations were carried out in the temperature range of 11řC to 21řC both during cooling and heating. We found that a phenomenon of hysteresis, i.e. alternate means of changes in velocity straight linear and lateral head displacement was obtained both at cooling and at heating. This phenomenon was not found for other parameters examined.

287/2523

Tytuł oryginału: Proteasome localization and ultrastructure of spermatozoa from patients with varicocele - immunoelectron microscopic study.
Autorzy: Ziemba H[alina], Biały Ł. P., Frącki S[tanisław], Bablok L[eszek], Wójcik C[ezary]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.169-170, il., bibliogr. 16 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Localization of proteasomes in spermatozoa from patients with varicocele-associated sterility was studied by means of immunolabeling with the MPC21 monoclonal antibody detecting the C3 subunit of the 20S proteasome. The reaction was visualized for electron microscopy using the secondary Nano-Gold-coupled antibody with Gold-Enhancement in pre-embeding technique. We found that semen samples from varicocele patients contained a large amount of abnormal spermatozoa characterized by the presence of dispersed chromatin and large residual bodies (cytoplasmic droplets) as well as spermatids at various stages of spermiogenesis. In normal spermatozoa, the immunolabeling was found in the acrosome, postacrosomal regions, nuclear vacuoles, in the neck and in the middle-piece as well as in the residual bodies, while chromatin remained unlabeled. In varicocele spermatozoa, the immunolabeling was also associated with chromatin and large residual bodies (cytoplasmic droplets). In contrast to normal, mature spermatozoa, the chromatin of the cells at earlier stages of spermiogenesis was strongly immunolabeled. The association of proteasomes with sperm chromatin and large residual bodies can be the sign of abnormality and distrubances in spermatogenesis associated wtih varicoc\ele.

288/2523

Tytuł oryginału: Stages of the rat thymic medulla development in foetal period.
Autorzy: Brelińska R[enata], Ostalska D[anuta], Kaczmarek E[lżbieta], Kowalska K[atarzyna]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.171-172, il., bibliogr. 6 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zarodki

Streszczenie angielskie: Development of thymic medulla was examined on consecutive gestational days (GD) in Wistar rats. Medullary thymic epithelial cells (TEC) were identified by immunocytochemical localisation of neuronspecific enolase (NSE). Organisation of thymic medullary architecture was determined by interaction of thymocytes with NSE-positive TEC, that led to formation of lymphoepithelial complexes (GD 19), in which the cells exhibited proliferative activity or traits of apoptosis. The studies indicated that differentiation events and organisation of thymic medulla require stagae-specific interactions between TEC and thymocytes.

289/2523

Tytuł oryginału: Age-related changes in rat thymic epithelial cells.
Autorzy: Brelińska R[enata], Jaroszewicz A., Kowalska K[atarzyna]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.173-174, il., bibliogr. 10 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We investigated age-related changes in immunocytochemical localisation of cytokeratin 16 (CK 16) in thymuses of female Wistar rats at various stages of adult life (months 1, 3, 6, 12). Within the 1 st month of life, distribution of CK typical for individual subsets of thymic epithelial cells (TEC) was observed. The most numerous CK16+ TEC were observed in the outer region of medulla, in the outer cells of Hasall's corpuscles and in the superficial epithelial layr neighbouring the connective tissue of the capsule, septa and vessels of the thymus. In the 3rd month of life, increased intensity of CK16 reaction in superficial TEC was accompanied by increased numbers of CK6+ TEC in the outer region of the medulla. Age-related alterations in the distribution of the studied markers were evident beginning from the 6rh month of life and involved increased expression of CK16 in the superficial layer of TEC, which at the interface with the septa formed startifed epithelium. In parallel, decreased numbers of CK16+ TEC distribution of a similar type developed in 12-month old rats and they probably relfected altered functions of some TEC populations and decreased or increased biological activity of other TEC populations.

290/2523

Tytuł oryginału: Lympho-epithelial interactions in rat thymus during pregnancy.
Autorzy: Brelińska R[enata], Kowalska K[atarzyna], Paczkowska A., Jaroszewski J[an]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.175-176, il., bibliogr. 8 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Alterations in the thymic epithelial cell activity were analysed during pregnancy and lactation in Wistar rats by examining the presence and in situ distribution of lymphoepithelial complexes formed by thymic nurse cells (TNC). TNC were identified in paraffin sections by their exprssion of MHC class II antigens, CD54 molecule and a neuromarker, protein gene product 9.5 (PGP9.5). On the first days of pregnancy (gestational days, GD) the numbr of PGP9.5+ TNC was found to decrease abruptly. On GD14, a transient increase was noted in the number of PGP9.5+, MHC+, CD54+ TNC. Another increase was observed in the course of lactation, when the weight of the thymus reached the lowest values. While the increase in TNC numbers during lactation may be linked to the process of reconstruction of teh thymic lymphoid population, the augmented activity of lymphoepithelial interactions on GD14 may be associated with thymic engagement in pregnancy-induced immune processes.

291/2523

Tytuł oryginału: The dynamics of morphological changes in the pyloric endocrine cells of rats with uremia.
Autorzy: Kasacka I[rena], Azzadin A[rsalan], Malla H.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.177-178, il., bibliogr. 14 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Disturbances in renal homeostatic function lead to changes in endorine cell secretory activity. The aim of this study was the histomorphological estimation of dependence of gastric APUD system cell morphology and function on the time after subtotal nephrectomy in Wistar rats. Fragments of gastric pylorus were collected 1, 2, 4, and 6 weeks after nephrectomy. Paraffin sections were stained with H + E and by silver impregnation. Immunohistochemical reactions with the use of specific antibodies against calcitonin gene-related peptide (CGRP), synaptophysin (SPh), somatostatin (ST), and neuron-specific enolase (NSE) were also perforemd. Immunoreactivity of the examined substances in the pyloric mucosa in the first week after nephrectomy was lower than in the control group. However, in the following time intervals, endocrine cells showed stonger immunostaining in comparison with the control rats. The results suggest that chronic renal failure can modulate secretory activity of APUD system cells.

292/2523

Tytuł oryginału: Preliminary evaluation of pancreatic islets in rats with experimental uremia and after thyroparathyroidectomy.
Autorzy: Kasacka I[rena], Azzadin A[rsalan], Sawicki B[ogusław]
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.179-180, il., bibliogr. 13 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Hormonal disorders are the permanent symptoms of renal failure. They concern all known hormones and can be due to quantitative changes of the secretory activity and disturbances of endocrine cell functions. The aim of this study was to establish whether experimental thyroparathyroidectomy in uremic animals causes detectable histomorphological changes in endocrine cells of pancreatic islets. Thyroparathyroidectomy was performed in rats 30 days after nephrectomy. Fragments of pancreatic tissue were collected 14 days after the operation. Paraffin sections were stained with H + E and by silver salt impregnation. Immunohistochemical reactions were conducted using antibodies against calcitonin gene-related peptide (CGRP), synaptophysin (SPh), somatostatin (ST), neuron-specific enolase (NSE), and chromogranin (CgA). It was shown that endocrine cells of pancreatic islets in thyroparathyroidectomized rats show intensified immunoreactivity to SPh and ST as compared to the control group of animals. Immunocytochemical reactions for NSE, CgA, and CGRP were negative.

293/2523

Tytuł oryginału: Thyroid C cells in male and female rats with chronic renal failure.
Autorzy: Kasacka I[rena], Azzadin A[rsalan], Malla H.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.181-182, il., bibliogr. 14 poz. - 37 Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików Kraków 02-05.09. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The kidneys are responsible for iodine and of thyroid hormone biodegradation. The aim of this study was the histomorphological and immunohistochemical evaluation of the influence of sex on parafollicular thyroid C cells in rats chronic renal failure. The experiment included 40 Wistar rats after subtotal nephrectomy, after sham operation, and without any surgical procedure. Two weeks after nephrectomy, fragments of thyroids were collected from the examined animals. Paraffin sections were stained with H + E and by silver impregnation. Calcitonin (CT), synaptophysin (SPh), somatostatin (ST), and neuron-specific enolase (NSE) were detected immunohistochemically in C cells. In rats with experimental uremia, immunostaining for the examined substances increased significantly in comparison to the controls. We also observed higher number of C cells with a stronger reaction in the group of males, compared to the female rats.

294/2523

Tytuł oryginału: Ultrasound and biochemical evaluation of liver regeneration process after resections for primary and metastatic tumors.
Autorzy: Kulig Jan, Nowak Wojciech, Popiela Tadeusz, Sobocki Jacek, Zarębski Bartosz
Źródło: Hepatogastroenterology 2002: 49 (44) s.349-353, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 304,355

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: background/Aims: The liver regeneration process after resective procedures is an important clinical problem especially in view of the growing population of patients requiring liver transplantations or undergoing resective procedures as well as those with toxic or viral liver injures. The aim of the study was to analyze liver regeneration potential using ultrasound examination, and liver function biochemical tests in the patients after liver resections. Methodology: Twenty-one patients with primary or metastatic liver cancer were included in the study (17 males, 4 females, mean age: 59.8, SD 10:3). The patients were undergoing gastrointestinal tract primary tumor resections with extended lymphandenectomy and liver metastatic tumor excisions. Liver volume was measured postoperatively in the follow-up ultrasound examinations and biochemical tests were performed before and after the procedure. Liver function and activity of p-450 cytochrome was tested with IRIS 13C stable isotope breath analyzer. Results: The mean time of follow-up was 12.6 months (SD 7.8) during which volume of liver increased on average in the first five months to 105.7, 112.1, 125.7, 131.2, and 138.4 p.c. of the immediate postoperative value. Biochemical parameters returned to normal after 2-3 weeks. Four patients in whom liver failed to regenerate died due to cancer spread in the mean time of 6.5 months. Conclusions: Liver has tremendous regeneration potential. In spite of this fact, extended hepatectomies affect largely the functionaln status of residual parenchyma which may lead to a fatal outcome.

295/2523

Tytuł oryginału: Chemokine growth-regulated-ŕ: a possible role in the pathogenesis of endometriosis.
Autorzy: Szamatowicz J[acek], Laudański P., Tomaszewska I., Szamatowicz M[arian]
Źródło: Gynecol. Endocrinol. 2002: 16 (2) s.137-141, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 313,072

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Endometriosis is a common disease in women at reproductive age. We investigated the concentration of neutrophil-activating factor (growth-regulated gene-ŕ, GRO-ŕ) (a member of the chemokine family), in peritoneal fluid of infertile women with or without endometriosis. Peritoneal fluid was obtained laparoscopically from 22 women with and 21 without visible endometriotic lesions. GRO-ŕ concentration was measured by the use of an ELISA kit. Median concentration of GRO-ŕ was 87.65 ń 56.19 pg/ml in the study group and 60.72 ń 11.98 pg/ml in the control group. The distribution of data differed from normal, therefore logarithmic transformation of data was performed. Concentration of GRO-ŕ was significantly higher in the peritoneal fluid of women with endometriosis when compared with controls (p = 0.05). No correlation between concentration of GRO-ŕ, stage of endometriosis, duration of infertility or sex steroid hormone levels was found. The study shows that GRO-ŕ may play a role in the pathogenesis of endometriosis, possibly by chemoattraction and activation of neutrophils present in higher numbers in the peritoneal fluid of women with endometriosis. It is also feasible that the angiogenic properties of GRO-ŕ might prompt the progression of endometriotic lesions.

296/2523

Tytuł oryginału: Clinical, electrophysiological and immunological remissions after thymectomy in myasthenia gravis.
Autorzy: Kostera-Pruszczyk A[nna], Emeryk-Szajewska B., Świtalska J., Strugalska-Cynowska H., Rowińska-Marcińska K[atarzyna], Nowak-Michalska T., Szyluk B.
Źródło: Clin. Neurophysiol. 2002: 113 (4) s.615-619, il., tab., bibliogr. s. 618-619
Sygnatura GBL: 311,330

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objectives: Approximately 50 p.c. of patients treated with thymectomy have a chance for symptom-free life. However, immunological and neurophysiological abnormalities may be detected in patients with clinical remission. Although improvement usually parallels decrease in acetylcholine receptor antibody (AChRAb) levels and jitter values, there is a question what factors influence immunological and electrophysiological remission in a population of myasthenia gravis (MG) patients. Methods: We analyzed retrospectively clinical data of 32 MG patients operated for generalized MG, followed-up at our department for 17.2 (4-31) years. They were in clinical remission for 12.8 (2-25) years. All of them had single fiber electromyography (SFEMG) of extensor digitorum communis muscle (EDC) muscle and estimation of AChRAb level at the end of follow-up. Their age at onset of MG was 17 years (6-48) and at thymectomy 19 (6.4-58) years. Tensilon test was positive in 30, repetitive nerve stimulation in 29 cases. Results: Clinical remission was reached on average 4.2 years after thymectomy. SFEMG jitter value normalized in 60 p.c. of cases, AChRAb were negative only in 34 p.c. of patients. Jitter values correlated with AChRAb levels (P = 0.006,, r = 0.5) but were not related to clinical factors. Only time to thymectomy correlated with time from thymectomy to clinical remission (P = 0.001, r = 0.5). Conclusions: Clinical remission is not always accompanied by normalization of SFEMG and AChRAb. Although normalization of neuromuscular transmission in patients with remission of MG is individual, short duration of MG before thymectomy increases the chance of early remission.

297/2523

Tytuł oryginału: Asymmetry of frameshift mutagenesis during leading and lagging-strand replication in Escherichia coli.
Autorzy: Gaweł Damian, Jończyk Piotr, Białoskórska Małgorzata, Schaaper Roel M., Fijałkowska Iwona J.
Źródło: Mutat. Res 2002: 501 (1/2) s.129-136, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Mutations in DNA, including frameshifts, may arise during DNA replication as a result of mistakes made by the DNA polymerase in copying the DNA template strands. In our efforts to better understand the factors that contribute to the accuracy of DNA replication, we have investigated whether frameshift mutations on the Escherichia coli chromosome occur differentially within the leading and lagging-strands of replication. The experimental system involves measurement of the reversion frequency for several defined lac frameshift alleles in pairs of strains in which the lac target is oriented in the two possible directions relative to the origin of chromosomal replication. Within these pairs any defined lac sequence will be subject to leading-strand replication in one orientation and to lagging-strand replication in the other. Fidelity differences between the two modes of replication can be observed as a differential lac reversion between the two strains. Our results, obtained with a series of lac alleles in a mismatch-repair-defective background, indicate that for at least some of the alleles there is indeed a difference in the fidelity of replication between the two modes of replication.

298/2523

Tytuł oryginału: Decreased apoptosis and distinct profile of infiltrating cells in the nasal polyps of patients with aspirin hypersensitivity.
Autorzy: Kowalski M. L., Grzegorczyk J., Pawliczak R., Kornatowski T., Wagrowska-Danilewicz M., Danilewicz M.
Źródło: Allergy 2002: 57 (6) s.493-500, il., tab., bibliogr. 49 poz.
Sygnatura GBL: 302,302

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: Patients with aspirin-hypersensitive rhinosinusitis/asthma suffer from a severe form of hyperplastic rhinosinusitis with recurrent polyposis. We aimed to assess the presence of apoptotic cells in nasal polyps from aspirin-hypersensitive (AH) and aspirin-tolerant (AT) patients with rginosinusitis as related to the characteristis of local inflammation. Methods: Nasal polyps obtained from 16 AH patients and 36 AT patients (17 atopic and 19 nonatopic) were stained for eosinophils and metachromatic cells, and in parallel immunocytochemistry was performed to detect CD45RO+, HLA-DR+, CD8+ and CD68+ positive cells. Apoptotic cells were detected by a nick-end labelling technique, TUNEL. Results: The density of apoptotic cells in AH polyps (5.5 + 1.5 cells/mmý) was significantly lower as compared to both atopic (18.7 + 3.8 cells/mmý; P 0.02;) and nonatopic (21.3 + 5.2 cells/mmý; P 0.01) AT polyps. The number of eosinophils, mast cells, and CD45RO+ cells weer significantly increased in AH compared to AT polyps () 0.001), and the density of HLA-DR+ cells in AH patients was higher than in nonatopic (P 0.02), but not in atopic AT patients. While in AH patients the duration of rhinosinusitis correlated inversely with the number of apoptotic cells (r = - 0.67; P 0.04), in contrast, in AT atopic patients the duration of rhinosinusitis showed positive correlation with apoptosis (r = 0.89; P 0.003). Conclusions: We conclude, that decreased apoptosis of inflammatory cells in nasal polyps from ASA-hypersensitive patients, reflects a distinct mechanism of local inflammation and may be related to persistence and severity of the disease in these patients.

299/2523

Tytuł oryginału: Eotaxin but not MCP - 3 induces eosinophil influx into nasal fluid in allergic patients.
Autorzy: Górski P., Wittczak T., Walusiak J., Pałczyński C., Ruta U., Kuna P., Alam R.
Źródło: Allergy 2002: 57 (6) s.519-528, il., bibliogr. 36 poz.
Sygnatura GBL: 302,302

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bacground: Eotaxin and MCP-3 (CC chemokines), owing to their preferential action on eosinophils, seem to be the very importance in the patophysiology of allergic rhinitis and asthma. The purpose of this study was to examine the effect of intranasally administered eotaxin and MCP-3 after specific allergen priming on the influx of inflammatory cells and their soluble mediators into the nasal mucosa. Methods: Eotaxin and MCP-3 have been applied intranasally at the increasing doses of 1, 5 and 10 ćg to allergic patients after priming. The 'nasal pool' technique was used. The cell count and biochemical parameters in nasal lavage were evaluated before 30 min, and 4 and 24 h after the challenge with chemokines. Results: Both eotaxin and MCP-3 induced the increase in clinical 'score' lasting till 24 h. Eosinophil influx into nasal mucosa after provocation with eotaxin was also obsrved. The challenge with MCP-3 did not induce any significant changes in nasal lavage fluid. Conclusions: Eotaxin is likely to play an important role in the pathogenesis of allergic conditions in humans. MCP-3 did not induce inflammatory cell influx into nasal mucosa. The role of this chemokine in the pathogenesis of allergic inflammation is difficult to assess and requires further research.

300/2523

Tytuł oryginału: Oral cavity as a potential source of gastric reinfection by Helicobacter pylori.
Autorzy: Karczewska Elżbieta, Konturek Joanna E., Konturek Peter C., Cześnikiewicz Marta, Sito Edward, Bielański Władysław, Kwiecień Nina, Obtułowicz Wojciech, Ziemniak Witold, Majka Jolanta, Hahn Eckhart G., Konturek Stanisław J.
Źródło: Dig. Dis. Sci. 2002: 47 (5) s.978-986, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 310,037

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Helicobacter pylori (Hp) is a common pathogen colonizing the a gastric mucosa, but some reports indicated that it may also be found in the oral cavity, which could serve as a reservoir of the bacteria and a source of gastric reinfection. Accordingly, we aimed to study whether the oral cavity, particularly gingival pockets, are colonized by Hp and whether it could be the source of gastric reinfection. We studied 329 patients with dyspeptic symptoms (257 with chronic gastritis, 15 with gastric ulcer. and 57 with duodenal ulcer). The [**13C]urea breath test (UBT), gastroscopy, and Hp culture from gastric biopsies were carried out, and material was collected from the oral cavity (gingival pocket) for bacteriological culture and genomic DNA studies. The serum was obtained for anti-Hp IgG and anti-CagA assays and saliva for anti-Hp IgA determination using the ELISA technique. bacteria in material from gingival pockets and biopsies from the corpus and antrum of stomach of 30 DU patients before and after Hp eradication were also examined by PCR technique, using primers specific for 16S rNA. All Hp-positive patients (276) were subjected to one week of triple therapy (omeprazole 2 x 20 mg twice a day, clarithromycin 2 = 500 mg twice a day, and metronidazole 2 x 500 mg twice a day). The measurements descrived above were then repeated at four weeks and six months. Bacterkilogical culture showed the prsence of Hp in the material from oral cavity in about 50 p.c. of patients, whereas UBT, used as a gold standard, revealed gastric Hp infection in about 84 p.c. of these patients...

301/2523

Tytuł oryginału: Functionally impaired TR mutants are present in thyroid papillary cancer.
Autorzy: Puzianowska-Kuźnicka Monika, Krystyniak Agnieszka, Madej Agnieszka, Cheng Sheue-Yann, Nauman Janusz
Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (3) s.1120-1128, il., tab., bibliogr. 52 poz.
Sygnatura GBL: 310,620

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: TRs are transcription factors that regulate cell proliferation, differentiation, and apoptosis. They are cellular homologs of the transcriptionally inactive viral oncogene v-erbA. We tested the hypothesis that the functions of TRs could be impaired in cancer tissues as a result of aberrant expression and/or somatic mutations. As a model system, we selected human thyroid papillary cancer, in which the most common abnormalities, RET/papillary thyroid cancer rearrangements (fusion of RET kinase domain to the activating domains of other genes), were found in 40-45 p.c. of cases. We found that the mean expression levels of TRá mRNA and TRŕ mRNA were significantly lower, whereas the protein levels of TRá1 and TRŕ1 were in cancer tissues than in healthy thyroid. Sequencing of TRá1 and TRŕ1 cDNAs, cloned from 16 papillary cancers, revealed that mutations affected receptor amino acid sequences in 93.75 p.c. and 62.5 p.c. of cases, respectively. In contrast, no mutations were found in healthy thyroid controls, and only 11.11 p.c. and 22.22 p.c. of thyroid adenomas had such TRá1 or TRŕ1 mutations, respectively. The majority of the mutated TRs lost their trans-activation function and exhibited dominant negative activity. These findings suggest a possible role for mutated thyroid hormone receptors in the tumorigenesis of human papillary thyroid carcinoma.

302/2523

Tytuł oryginału: Microtubule associated protein (Tau) gene variability in patients with frontotemporal dementia.
Autorzy: Kowalska Anna, Takahashi Keikichi, Kozubski Wojciech, Tabira Takeshi
Źródło: Folia Neuropathol. 2002: 40 (1) s.1-5, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Frontotemporal dementia represents up to 10 p.c. of all dementias and is, next to Alzheimer's disease and Lewy body disease, the third most common cause of degenerative dementia. The term "frontotemporal dementia" covers a range of conditions, icluding Pick's diseases, frontal lobe degenration and dementia associated with motor neurone disease. Neuropathologically FTD is characterised by atrophy of the frontal and temporal lobes of the cerebral cortex, often with additional subcortical changes. Both familial and more frequently sporadic forms of FTD can be recognised. Recently, mutations in the microtubule-associated protein (tau) gene have been found in families with frontotemporal dementia and parkinsonism linked to chromoseme 17 (FTDP-17). The identification of mutations in the tau gene indicates that the protein plays a central role in the process of neurodegeneration. Epidemiology of frontotemporal dementias in Poland remains still unknown. A prevalence of tau mutations among Polish patients has not been established yet. Here, we report results of a mutational analysis of the tau gene among FTD patients. No pathogenic mutation was found in the analysed sample. The study confirmed that the frequency of tau mutations is very low and depends strongly on the clinical criteria used to select patients. Mutations in the tau gene account only for a small number of FTD cases with a clear autosomal dominant pattern of disease inheritance. Therefore there should exist additional genetic and non-genetic factors contributing to the pathogenesis of both familial (linked and non-linked to chromosome 17) and sporadic forms of FTD.

303/2523

Tytuł oryginału: The effect of thymus ubiquitin complex on neuropathological changes within the brain of pregnant rabbits with experimental model of antiphospholipid syndrome.
Autorzy: Nowacki Przemysław, Ossowicka-Stępińska Jadwiga, Ronin-Walknowska Elżbieta
Źródło: Folia Neuropathol. 2002: 40 (1) s.7-11, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: The present study was designed to assess the impact of thymus ubiquitin complex (TUC) on morphological changes within the central nervous system (CNS) in pregnant rabbits with experimental antiphospholipid syndrome (APS). We also compared the intensity of neuropathological appearance and serological markers of the APS treated with the TUC. Post-mortem neuropathological investigations were done in 47 female New Zealand rabbits. The material was divided into 4 groups: Group I - 23 pregnant animals with APS; Group II - 7 pregnant rabbits with APS, treated with TUC; Group III - 7 pregnant rabbits without APS, treated with TUC; Group IV (the control one) - 10 pregnant animals without APS, intreated with TUC. The APS was induced by subcutaneous injections of cardiolipin. There were two main abnormalities within the CNS of pregnant rabbits, which could be a result of the APS: the thrombo-necrotic foci of nervous tissue, and perivascular or meningeal inflammatory infiltrates. It turned out that TUC application reduced the morphological abnormalities, but it did not eliminate them entirely. The number of cases with thrombo-necrotic changes was reduced by approximately 18 p.c., while perivascular infiltrates by 36 p.c. respectively. The TUC application did not influence the meningeal infiltration. The platelet count significantly increase (p 0.001), the activated partial thromboplastin time shortened (p 0.001), and the number of immunised animals, demonstrating positive immunofluorescence test, significantly decreased after the TUC administration...

304/2523

Tytuł oryginału: N-methyl-d-aspartate receptor-mediated processing of á-amyloid precursor protein in rat hippocampal slices: in vitro - superfusion study.
Autorzy: Gordon-Krajcer Wanda, Salińska Elżbieta, Łazarewicz Jerzy W.
Źródło: Folia Neuropathol. 2002: 40 (1) s.13-17, il., bibliogr. 26 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Abnormal proteolytic degradation of the á amyloid precursor protein (á-APP) may result in accumulation of potentially neurotix á amyloid (áA). The role of various receptors in the regulation of á-APP processing has been suggested. This study aimed to determine how NMDA receptors and Caý+ ions regulate proteolysis of á-APP in rat hippocampus in vitro. Adult rat hippocampal slices were superfused with NMDA-containing media, and immunoreactivity of soluble á-APP derivatives was detected in dialysates. Application of 100 ćM and 250 ćM NMDA for 20 min in Caý+-containing medium induced dose-dependent release of amino-terminal á-APP derivatives, and a fragment of Aá sequence, whereas carboxy-terminal fragments of á-APP were only slighty detected. This indicates activation of á-APP processing, and release of its soluble cleavage products. This effect was inhibited by NMDA receptor antagonist 1 ćM MK-801 and 100 ćM CPP in Caý+-free medium, thus indicating that NMDA receptors and calcium ions mediate proteolytic non-amyloidogenic degradation of the á-APP.

305/2523

Tytuł oryginału: Early infantile form of spinal muscular atrophy (Werdnig-Hoffmann disease) with prolonged survival.
Autorzy: Borkowska Janina, Rudnik-Schoneborn Sabine, Hausmanowa-Petrusewicz Irena, Zerres Klaus
Źródło: Folia Neuropathol. 2002: 40 (1) s.19-26, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: According to several previously used classifiaction systems, spinal muscular atrophy (SMA) type I has been characterised by an early onset ( 6 months), severe course (patients are never able to sit without support) and fatal prognosis (death before 2-4 years). We report 36 out of 349 SMA type I patients (10 p.c.) who had an onset before 6 months and never learnt to sit but sirvived at least beyond their fifth birthday, in spite of total immobility and frequent respiratory infections. These data support a classification avoiding age at death as a criterion. In the subgroup of type I with prolonged survival, there was no difference in life span between those individuals who had an onset of disease at birth or before and those with symptoms starting after 3 months. Life span may be related to the ability to withstand repeated respiratory insult, as several of the sibships with children affected by similar neuromuscular compromise showed discordant ages at death. "Chronic" SMA I and SMA II may represent overlapping phenotypes as our index patients with SMA I had a sib with SMA.

306/2523

Tytuł oryginału: Suicide muscle cell programme-apoptosis. Ultrastructural study.
Autorzy: Fidziańska Anna
Źródło: Folia Neuropathol. 2002: 40 (1) s.27-32, il., bibliogr. 20 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

niemowlęta

Streszczenie angielskie: To establish the ultrastructural criteria of suicide muscle cell programme-apoptosis we investigated the muscle biopsies of acute fatal spinal muscular atrophy (SMA) infants with gene survival motor neurone (SMN) exon 7 deletion. The muscle cell apoptosis was characterised by precisely sequential morphology of both cell nucleus and sarcoplasm. The most characteristic feature was the aggregation of nuclear chromatin initially into peripherally located ring and later into uniformly dense, dark masses. Accompanying features included sarcoplasmic condensation cell shrinking and fragmentation into multiple bodies. The coexistence of muscle cells at different stages of apoptosis in the same tissue section was the most prominent and specific features of suicide muscle cell progarmme. The appearance of an identical sequence of ultrastructural changes in muscle as observed in other tissues suggesrs that a unique mechanism is involved in genetically programmed cell death.

307/2523

Tytuł oryginału: CT angiography in the diagnosis of spontaneous extracerebral vertebral artery dissection.
Autorzy: Pniewski Jarosław, Woźniak Radosław, Chodakowska-Żebrowska Małgorzata, Sikorska Jolanta, Walecki Jerzy
Źródło: Folia Neuropathol. 2002: 40 (1) s.33-37, il., bibliogr. 20 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Spontaneous vertebral artery dissection is an underrecognised cause of cerebral ischaemia, most often seen in young adult patients. For this diagnosis cerebral angiography is the most sensitive and specific method. We describe a 30-year0old, male, with cerebrallar stroke, in whom spontaneous vertebrak artery dissection was diagnosed. Our diagnosis was confirmed by cerebral angiography and CT angiography. In our case anticoagulation (in acute phase) was introduced subcutaneously and later continued orally. The outcome was very good with complete remission. CT angiography seems to be a promising non-invasuve method for the diagnosis of vertevral artery dissection. The prognosis in the patient with this diagnosis is relatively good (if managed properly), but the disease must always be treated as serious and life-threatening.

308/2523

Tytuł oryginału: Early occurring neuronal migration and maturation disorders.
Autorzy: Dąmbska Maria
Źródło: Folia Neuropathol. 2002: 40 (1) s.39-42, il., bibliogr. 6 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: This case report presents severe malformations of the central nervous system as a result of a pathological process occurring very early during development. Abnormal maturation and migration of neuroblasts were seen in all cerebral structures. Such changes are most often genetically determined, but in our case the analysis of their aetiology was not available.

309/2523

Tytuł oryginału: In memory of Professor Hanna Jędrzejowska [1930-2001].
Autorzy: Dąmbska Maria
Źródło: Folia Neuropathol. 2002: 40 (1) s.43-44, il. 2002
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Jędrzejowska

Hanna 1930-2001

310/2523

Tytuł oryginału: In memory of Professor Mirosław Mossakowski [1929-2001].
Autorzy: Dąmbska Maria
Źródło: Folia Neuropathol. 2002: 40 (1) s.45-46, il.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Mossakowski

Mirosław 1929-2001

311/2523

Tytuł oryginału: Salvage hemipelvis radiotherapy with fertility preservation in an adolescent with recurrent vulvar carcinoma.
Autorzy: Serkies Krystyna, Wysocka Barbara, Emerich Janusz, Hrabowska Maria, Jassem Jacek
Źródło: Gynecol. Oncol. 2002: 85 (2) s.381-383, bibliogr. 10 poz.
Sygnatura GBL: 305,822

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background. To preserve fertility, hemi-pelvis irradiation was chosen for locoregional groin recurrence of vulvar cancer in an adolescent. Case. A case of squamous cell vulvar carcinoma in uncommonly young patient is presented. First surgical management (local excision of T1N0M0 tumor of the right labia minora) was performed at the age of 16.5 years. Further therapy included wide local resection of recurrent local lesion, two subsequent ipsilateral groin dissections of nodal metastases (with extranodal spread at the first instance), and ipsilateral pelvic lymphadenectomy. The patient was administered external beam irradiation of 45 Gy to the hemipelvis followed by 10.8 Gy boost to the right inguinal region. After 6.5 years from the completion of radiotherapy the patients is free of disease. She managed to conceive but the labor was premature and the infant died in its 7th week pf life. Conclusion. In this case salvage hemi-pelvic irradiation for groin metastases of vulvar cancer has proved to be an effective treatment, allowing preservation of hormonal and obstetric functions. Partial post-irradiation damage of the uterus might have caused premature labor. Thus, special obstetric care is advisable in such situations.

312/2523

Tytuł oryginału: Expression of the multidrug resistance-associated protein (mrp) gene in chronic lymphocytic leukemia.
Autorzy: Juszczyński Przemysław, Niewiarowski Wojciech, Krykowski Euzebiusz, Robak Tadeusz, Warzocha Krzysztof
Źródło: Leuk. Lymphoma 2002: 43 (1) s.153-158, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In order to define more accurately the role of multidrug resistance (MDR)-related protein (mrp) gene in chronic lymphocytic leukemia (CLL), we addressed the question of its expression pattern in isolated peripheral blood B lymphocytes in seven healthy donors and 28 patients with CLL, with respect to some laboratory and clinical parameters of the disease. For this purpose, we used a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), based on the coamplification of an internal standard not homologous to the DNA target to quantify the mrp transcription level in each studied sample. We report that the level of constitutive mrp gene's expression in peripheral blood lymphocytes is higher in CLL patients than in healthy controls. We found increased mrp gene expression levels in patients with higher white blood cells (WBC) and lymphocytes' counts as well as in more advanced disease stages according to Rai or Binet scale. Finally, mrp gene's expression was higher in patients with progressive CLL, especially in cases refractory to chemotherapy salvage. The results of the present study suggest that expression of mrp gene might be relevant in the pathogenesis of the MDR phenotype in CLL.

313/2523

Tytuł oryginału: The role of nucleoside analogues in the treatment of chronic lymphocytic leukemia-lessons learned from prospective randomized trials.
Autorzy: Robak Tadeusz
Źródło: Leuk. Lymphoma 2002: 43 (3) s.537-548, il., tab., bibliogr. 78 poz.
Sygnatura GBL: 312,939

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The newer purine nucleoside analogues (PNA), fludarabine (FAMP) and cladribine (2-chlorodeox-yadenosine, 2-CdA) have been synthesized recently and introduced into the treatment of chronic lymphocytic leukemia (CLL). The results of large phase II studies indicate that FAMP and 2-CdA are similarly active in CLL. Unfortunately, no prospective randomized study comparing the results of the treatment of CLL patients with FAMP and 2-CdA has been published so far. Significantly higher overall response (OR) and complete remission (CR) in patients treated initially with PNA than with chlorambucil or cyclophosphamide based combination regimens has been recently confirmed in five prospective multicentre randomized trials. These studies have also shown longer response duration in patients treated with PAN than with conventional chemotherapy. Overall survival progression free and events free survival were similar in patients treated with ONA and with chlorambucil or other alkylating agent based regimens. However, the majority of randomized trials were designed as cross studies and most patients, treated with conventional chemotherapy were given PNA when refractory or in early relapse, which may influence the survival time. The results of a randomized study have shown a higher incidence of neutropenia and infections in patients treated with PNA than with chlorambucil. However, the frequency of autoimmune hemolytic anemia, pure red cell aplasia, secondary neoplasms and Richter's syndrome seems to be similar in patients treated with PNA and standard alkylating agents based chemotherapy...

314/2523

Tytuł oryginału: Intercalation of imidazoacridinones to DNA and its relevance to cytotoxic and antitumor activity.
Autorzy: Dzięgielewski Jarosław, Ślusarski Bartłomiej, Konitz Antoni, Składanowski Andrzej, Konopa Jerzy
Źródło: Biochem. Pharmacol. 2002: 63 (9) s.1653-1662, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Imidazoacridinones (IA) are a class of antitumor agents which includes C-1311, an interesting drug in clinical trials. This study investigated the mechanism of IA binding to DNA for a series of 13 analogs that differ in their cytotoxic potency. Using C-1311 as a model compound, crystallographic, spectroscopic and biochemical techniques were employed to characterize drug-DNA interactions. X-ray crystallographic analysis revealed a planar structure of imidazoacridinone core that is capable of intercalative DNA bibding. Accordingly, C-1311 binding to DNA followede "classical" pattern observed for intercalation, as proved by tha DNA topoisomerase I - unwinding experiments, with relatively weak binding affinity (Ki = 1.2 x 10**5 M-1), and the binding site size of 2.4 bp. Other IA also bound to DNA with the binding affinity in the range of 10**5 M-1 and binding site size of 2-3 bp, suggesting a prevalence of the intercalative mechanism, similar to C-1311. Considerable DNA binding affinity was displayed by all highly cytotoxic derivatives. However, none of the analyzed drug-DNA binding parameters was significantly correlated with IA biological activities such as cell growth. DNA and RNA synthesis inhibition, or tumor growth inhibition, which suggests that the IA ability to non-covalently bind to DNA is not crucial for their biological activity. These results show that the ability to intercalate into DNA is a prominent attribute of IA, although factors other intercalative binding seem to be required for the biological activities of IA drugs.

315/2523

Tytuł oryginału: Inhibition of gluconeogenesis by vanadium and metformin in kidney-cortex tubules isolated from control and diabetic rabbits.
Autorzy: Kiersztan Anna, Modzelewska Aleksandra, Jarzyna Robert, Jagielska Elżbieta, Bryła Jadwiga
Źródło: Biochem. Pharmacol. 2002: 63 (7) s.1371-1382, il., tab., bibliogr. 75 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: Effect of vanadyl acetylacetonate (VAc) and metformin on gluconeogenesis has been studied in isolated hepatocytes and kidney-cortex tubules of rabbit. Glucose formation from alanine + glycerol + octanoate, pyruvate or dihydroxyacetone was inhibited by 50-80 p.c. by 100 ćM VAc or 500 ćM metformin in renal tubules of control and alloxan-diabetic animals, while the inhibitory action of these compounds in hepatocytes was less pronounced (by about 20-30 p.c.). In contrast to VAc, metaformin increased the rate of lactate formation by about 2-fold in renal tubules incubatet with alanine + glycerol + octanoate. In view of VAc-induced changes in intracellular gluconeogenic intermediates and gluconeogenic enzyme activities, it is likely taht this compound may decrease fluxes through pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase. In contrast to VAc, metformin-induced decrease in renal gluconeogenesis may result from a decline of cytosolic oxaloacetate level and consequently PEPCK activity. Following 6 days of VAc administration (1.275 mg V kg-1 body weight daily) the blood glucose level in alloxan-diabetic rabbits was normalised while blood glucose changes in control animals were not observed. On the contrary, in diabetic animals treated for 6 days with metformin (200 mg kg-1 body weight day -1) a high blood glucose level was maintained. Unfortunately, VAc-treated control and diabetic rabbits exhibited elevated serum urea and creatinine levels. In VAc-treated animals vanadium was accumulated in kidney=cortex up to 7.6 ń 0.6 ćg V g-1 dry weight. In view of a potential vanadium nephrotoxicity...

316/2523

Tytuł oryginału: Components of the IFN-ç signaling pathway in tumorigenesis.
Autorzy: Blanck George
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.151-158, il., bibliogr. 78 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Many features of the interferon ç (IFN-ç) signaling pathway would suggest that it is anti-tumorigenic. The IFN-ç signaling pathway leads to apoptosis and to the expression of immune function proteins that could cooperate with T cells in the destruction of tumor. Various lines of experimental approach have, in general, supported the hypothesis that the IFN-ç signaling pathway is anti-tumorigenic. However, data also indicate that the idea that the IFN-ç signaling pathway is exclusively anti-tumorigenic is too simplastic. Also, to date, very ;ittle of the knowledge regarding the anti-tumor effects of the IFN-ç pathway has been useful in the prognosis or therapy of cancer. This review summarize the current state of knowledge regarding the IFN-ç signaling pathway in tumorigenesis, with an emphasis on MHC class II induction in tumor cells and the induction of apoptosis in tumor cells. The review also indicates some future areas of investigation that offer hope for applying this knowledge in reducing cancer nortality.

317/2523

Tytuł oryginału: Modulation of pulmonary innate immunity during bacterial infection - animal studies.
Autorzy: Schultz Marcus J., Poll Tom van der
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.159-167, il., tab., bibliogr. 68 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Both the increasing number of immunocompromised patients susceptible to pneumonia and the development of bacterial resistance are sgnificant problems related to the treatment of pneumonia. The primary outcome of treatment for pneumonia is to tip tha balance a successful host response. An ideal approach would be a combination of immunomodulayion and conventional antimicrobial therapy. It is of increasing imoprtance to understand the components of innate immunity, before immunomodulatory therapy can be applied to patients. Much of our knowledge of the role of alveolar macrophages, cytokines and chemokines in the pathogenesis of pneumonia is derived from animal studies on experimental pneumonia. This article summarizes current information on the role of an alveolar macrophage (AM) and AM-derived mediators in host defense against pneumonia.

318/2523

Tytuł oryginału: CD28 costimulatory molecule - expression, structure and function.
Autorzy: Boćko Dorota, Kosmaczewska Agata, Ciszak Lidia, Teodorowska Renata, Frydecka Irena
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.169-177, il., bibliogr. 94 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: T cell activation is a key in triggering an antigen specific immune response of the organism. The process is induced primarily by the signal generated by direct interaction of a T cell receptor with an antigen bound to the major histocompatibile complex on an antigen-presenting cell (APC). Although the signal is critical in exciting immune response, an additional, costimulating signal is required. The major second signal is generated by interaction of the CD28 molecule expressed on most T lymphocytes with its natural ligands CD80 and CD86 located on APCs. The signal excited by CD28 triggering involves multiple second-messenger cascades, leading to the activation of transcription factors and finally results in cell proliferation, cytokine production, and the generation of effector functions. The importance of CD28-delivered costimulatory signals was proven in experiments with CD28-deficient mice. T cells from these mice exhibited an impaired pattern of cytokine secretion and defects in T cell-dependent antibody production. Certain forms of immunopathology might result from the aberrant regulation of CD28 expression.

319/2523

Tytuł oryginału: Mast cells and inflammation.
Autorzy: Stassen Michael, Hltner Lothar, Mller Christian, Schmitt Edgar
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.179-185, bibliogr. 52 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Mast cells have long been recognized as potent producers of a large panel of biologically highly active mediators such as biogenic amines, arachidonic acid metabolites, cytokines and chemokines, but most of their biological functions have been elusive speculative. By taking advantage of mast cell-deficient mice, the role of mast cells in a variety of experimental settings can now studied in detail and such approaches have dramatically altered and enlarged our knowledge about mast cell biology and function. Herein we will focus on the role of mast cells in inflammatory reactions of diverse origin, such as delaved type hypersensitivity, atopy, immune complex-mediated inflammation and innate immune responses. From the current standpoint, there is no doubt that the most outstanding and beneficial feature of mast cells is their recently discovered ability to induce a life-saving inflammatory response rapidly upon encountering microbes and microbial constituents. Nevertheless, the picture is also emerging that mast cells are deeply involved in the induction and maintenance of a variety of severe allergic and autoimmune diseases. However, a deeper understanding of their activation and immune-modulatory capacity might open a new window for the development of curative strategies.

320/2523

Tytuł oryginału: The role of dendritic cells in neurodegenerative diseases.
Autorzy: Iribarren Pablo, Cui You-Hong, Le Yingying, Wang Ji Ming
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.187-196, il., bibliogr. 92 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) involved in the induction of adaptive immune responses. The presence of DCs in the central nervous system (CNS) and the active participation of the immune system in a variety of neurodegenerative diseases have been demonstrated. This review will discuss recent findings pertinent to DCs and other antigen-presenting cells in the CNS in health and disease states.

321/2523

Tytuł oryginału: Active vaccination after allogeneic bone marrow cell transplantation: a new option in the immunotherapy of cancer?
Autorzy: Zller Margot, Matzku Siegfried
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.197-224, bibliogr. 456 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The concept of immunotherapy of cancer was evoked more than a century ago by W. Coley. Yet it is only recently that the state of knowledge allows for molecularly defined therapeutic approaches and much effort will still be required to place immunotherapy beside of surgery, chemotherapy and radiation as a fourth option. In this review, we will focus chiefly on two aspects: active therapeutic vaccination, because it is our belief that this approach will provide a major breakthrough, and the potential efficacy of combining active vaccination with allogenic bone marrow cell transplantation. It was recently established in clinical trials that allogenic bone marrow cell transplantation does not require myeloablative conditioning. Non-myeloabaltive conditioning which avoids the high toxicity of the conventional approach, it is only kind which allows the recruitment of elderly patients and patients in poor health. Concerning active vaccination protocols, we will address the questions 1) what the targets (i.e. the Antigens) of immunotherapeutic approaches could be; 2) how to achieve an optimal confrontation of the immune system with these tumor-associated antigens; and 3) which response elements are needed for raising a therapeutically successful immune reaction against these antigens. Many question remain to be answered in the field of allogeneic bone marrow transplantation after non-myelablative conditioning to optimize the therapeutic setting for this, most likely, very powerfull tool of cancer therapy. We will briefy summarize current considerations to improve engraftment...

322/2523

Tytuł oryginału: A two step procedure to fractionate mouse testicular macrophages with different cytokine profiles.
Autorzy: Bryniarski Krzysztof, Szewczyk Katarzyna, Ptak Maria, Bobek Małgorzata, Ptak Włodzimierz
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (3) s.225-229, tab., bibliogr. 15 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Cells isolated enzymatically from the interstitial tissue of mouse male gonads are composed of macrophages, Leydig cells, and myofibroblasts. They can be separated on density gradients, either by sedimentation (Ficoll) or flotation (Percoll) into several fractions according to the different buoyant densities of the different cells in the mixture. Macrophages (FcçR+, esterase+) present in cell mixtures can by highly enriched (to 95 p.c. purity) in a single step by rosetting with opsonized erythrocytes followed by sedimentation on Lymphoprep. Separate fractions of highly purified (over 95 p.c.) macrophages obtained by the successive use of density gradients and rosetting differ significantly in the production of cytokines, e.g. cells from fractions at lower density produce little IL-6, cells from fractions at higher density are poor producers TNF-ŕ, while testicular macrophages (TMf) in intermediate fractions produce significant amounts of both cytokines. These differences may suggest that particlar subpopulations of testicular macrophages play different biological roles in the testis.

323/2523

Tytuł oryginału: Testing for aspirin hypersensitivity.
Autorzy: Bochenek G., Niżankowska E[wa], Szczeklik A[ndrzej]
Źródło: Allergy 2002: 57 (7) s.562-565, bibliogr. 33 poz.
Sygnatura GBL: 302,302

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

324/2523

Tytuł oryginału: Influence of vitamins C and E on cytotoxic activity of adriamycin in chosen cell cultures.
Autorzy: Woźniak Grażyna, Anuszewska Elżbieta L.
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.31-35, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Influence of different concentratioons of ascorbic acid (vitamin C) and dl-ŕ-tocopherol acetate (vitamin E) on in vitro cytotoxic adriamycin activity, in: embryonic human fibroblasts (CLV102), human melanoma cells (ME18) and adriamycin-resistant subline cells (ME18/R), was studied. IC50 value for each compound (compound concentration in the culture medium, for which 50 p.c. of cells survive) was determined. Cells' survival after the used agent was examined with trypan blue test. The relationship between different concentrations of vitamin C and toxicity of adiamycin, used at appropriate IC50 concentrations, was expressed for all the examined cells as their survival decrease, being in direct proportion to the concentration increase of this vitamin in the medium. In the case influence of vitamin E on adriamycin, the protective effect of this vitamin was observed in the concentration range: from 5 to 300 ćg/ml (p ó 0,0001), as an increase of the examined cell survival for ME18, ME18/R as well as for CLV102, comparing to the control (p = 0.05) without this vitamin. Paralelly, a statistically significant survival decrease was observed, if the concentration of vitamin E in the culture medium exceeded 500 ćg/ml. Received results showed different, in defined concentration range, effects of the vitamin C or vitamin E activity for adriamycin cytotoxicity. These effects were similar for all the examined cells.

325/2523

Tytuł oryginału: Synthesis of new N-substituted cyclic imides with expected anxiolytic activity. [P.] 21: Derivatives of 1,4-dibromo-dibenzo[e.h]bicyclo-[2.2.2]octane-2,3-dicarboximide.
Autorzy: Kossakowski Jerzy, Krawiecka Mariola, Perliński Mirosław
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.37-43, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The preparation is described of a number of new-N-substituted derivatives of dibenzo[e.h]bicyclo[2.2.2]octane-2,3-dicarboximide with potential anxiolytic activity.

326/2523

Tytuł oryginału: Investigation of interaction of promethazine with cyclodextrins.
Autorzy: Lutka Anna
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.45-51, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The effect of á-CD and its substituted derivatives (DM-á-CD and HP-á-CD) on the solubility and photostability of promethazine was investigated in solution and in the solid state. The soluble complexes of protonated (pH = 6.8) and basic (pH = 10.8) forms of promethazine with CDs were studied using the spectral method. The influence of pH and CD complexation on photostability of PM in solution was followed. It was found that the photochemical decomposition of promethazine (PM) alone and in the presence of CD procedes according to the first order reaction. It was also established that as well the presence of CD procedes according to the first order reaction. It was also established that as well the presence of CD as the acidic medium of reaction increased the photostability of PM in solution. Formation of solid inclusion complexes of PM with CDs was evaluated using FT IR, 13C NMR and DCS methods. The results obtained indicate that independently of the complexation method in the solid state (kneading or heating), the presence of CD decreases the solubility of PM; the reason may be that phenothiazine ring of PM did not enter into the cavity of á-CD and its derivatives.

327/2523

Tytuł oryginału: Flavonoid compounds from Pyrus communis L. flowers.
Autorzy: Rychlińska Izabela, Gudej Jan
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.53-56, il., tab., bibliogr. 11 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Five flavonoid glycosides: kaempferol 3-0-á-D-glucopyranoside (IV), isorhamnetin 3-0-á-D-glucopyranoside (V), isorhamnetin 3-0-á-D-(6'-0-ŕ-L-rhamnopyranosyl)-glucopyranoside (VIII), 8-methoxykaempferol 3-0-á-D-(2'-0-ŕ-D-glucopyranosyl)-glucopyranoside (VI), 8-methoxykaempferol 3-0-á-D-(2'-0-ŕ-L-rhamnopyranosyl)-glucopyranoside (VII) and chlorogenic acid (IX) were isolated from the flowers naturally growing Pyrus communis L. They were identified by chemical and spectroscopic methods.

328/2523

Tytuł oryginału: Flavonoids in the leaves with stems of some species of the Aquilegia L. genus.
Autorzy: Bylka Wiesława
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.57-60, tab., bibliogr. 7 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Methanolic extracts from the leaves with stems of seven species of Aquilegia L. genus (A. hybrida Scott-Eliot, A. olympica BOISS., A. flabellata SIEB et ZUCC., A. alpina L. atrata W.D.J. KOCH, A.vitoleili L., A. caerulea JAMES) were subjected to co-chromatography with the extract and the flavonoids obtained from A. vulgaris L. Moreover, the content of 4'-methoxyapigenin 6-C-glucoside (isocytisoside) was determined spectrophotometrically in these species after chromatographic separation.

329/2523

Tytuł oryginału: Optimization of the separation of some Chelidonium maius L. alkaloids by reversed phase high-performance liquid chromatography using cyanopropyl bonded stationary phase.
Autorzy: Petruczynik Anna, Gadzikowska Maria, Waksmundzka-Hajnos Monika
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.61-64, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The high-performance liquid chromatographic method using two stationary phases: RP-18 and cyanopropyl silica for the analysis of some alkaloids in Chelidonium maius L. extracts is performed. The extract was chromatographed using a mobile phase composed of an organic modiffer (acetonitrile, methanol, tetrahydrofuran or 1,4-dixan), phosphate buffer and sodium octadecylsulfate or di(2-ethyl hexyl) orthophosphoric acid (HDEHP) as ion-pairing reagents. The influence of the different kind of stationary phase and organic modifiers on retention has been compared. In order to improve the selectivity of separation and peak shape, gradient elution was applied.

330/2523

Tytuł oryginału: Effect of desogestrel on blood-lipid in relation to its biological activities.
Autorzy: Saha Achintya, Roy Kunal, De Kakali, Sengupta Chandana
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.65-69, tab., bibliogr. 35 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Desogestrel (DG), a 19-nor progestin, is widely used in replacement therapy as a contraceptive steroidal hormone. Considering the importance of its partition coefficient parameter (log P = 5.68), a significant contributor to its action mechanism, interactions of the drug with blood-lipids, had been investigated using phospholipid binding, fatty acid composition and peroxidation phenomena as the parameters under investigation to explain its pharmacodynamic behavior. From the present investigation, it was observed that lipid loss after incubation of whole blood with DG (80 ng/ml, effective contraceptive concentration in blood) for varying periods of time was accompanied with significant changes in fatty acid composition, which may be ascribed to binding affinity of DG with lipid constituens in blood that may have a role in the mediation of its therapeutic effect. Lipid peroxidation induction potential of DG has been quantitatively measured in the context of its toxicity. The results reveal that DG caused significant extent of lipid peroxidation. Ascorbic acid, an antioxidant, at equvalent human dose of 250 mg could significantly reduce DG-induced lipid peroxidation.

331/2523

Tytuł oryginału: Influence of captopril and enalapril on some central effects of ethanol.
Autorzy: Czarnecka Elżbieta, Pietrzak Bogusława
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.71-75, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The influence of captopril and enalapril on acute toxicity of ethanol, ethanol-induced hypothermia, ethanol sleeping time has been investigated in mice. Moreover, the combined effect of captopril and enalapril on spontaneous locomotor activity in mice has been examined. The captopril (5 and 20 mg/kg) and enalapril (5 and 20 mg/kg) were injected intraperitoneally i.p. The drugs were given as single or repeated doses for 10 days. It has been shown that the captopril and enalapril administered in single doses decreases, but chronic administration increase acute toxicity of ethanol. Captopril and enalapril in single doses enhanced, but chronic administration inhibits hypothermic effect of ethanol. Captopril and enalapril reduces ethanol sleeping time. Captopril and enalapril administered for 10 days and enalapril in a single dose 20 mg/kg decreases ethanol induced hyperactivity.

332/2523

Tytuł oryginału: Synthesis and tumoricidal activity evaluation of new morin and quercetin sulfonic derivatives.
Autorzy: Król Wojciech, Dworniczak Szymon, Pietsz Grażyna, Czuba Zenon P., Kunicka Mariola, Kopacz Maria, Nowak Dorota
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.77-79, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 301,378

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Flavonoids are a group of naturally occuring compounds with interesting medical properties, such as antiinflammatory, antiallergic, antibacterial and antitumor activities. In our experiments we were trying to examine the tumoricidal activity of newely synthesized derivatives of two flavonoids: 3,5,7,2',4' - pentahydroxyflavone (morin) and 3,5,7,3',4' - pentahydroxyflavone (quercetin). The derivatives were: natrium salt of morin-5'-sulfonic acid (NaMSA), natrium salt of quercetin-5'-sulfonic acid (NaQSA), complex of Mg2+ with quercetin-5'-sulfonic acid (QSA), complex of iron (II) with QSA. The antitumor activity of these agents was tested in vitro on two cell lines: L1210 - murine lymphocytic leukaemia and P-815 - murine mastocytoma. Our experiments showed that sulfonic derivatives of these two flavonoids were less potent than the original agents in their cytostatic and cytotoxic activities. However, their solubility in water was grweater than that of the original agents and higher cilture medium concentration of these derivatives was obtained. The results indicate that the ability of flavonoids to act tumoricidally is reciprocally correlated with their lipophilicity.

333/2523

Tytuł oryginału: The immunophenotype of patients with recurrent pregnancy loss.
Autorzy: Darmochwał-Kolarz Dorota, Leszczyńska-Gorzelak Bożena, Roliński Jacek, Oleszczuk Jan
Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 103 (1) s.53-57, tab., bibliogr. 17 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: The aim of the study was to estimate the alterations in the phenotype of lymphocytes of women with unexplained pregnancy failures in comparison with healthy women. Materials and methods: Fourten women with unexplained habitual miscarriages and 18 healthy, fertile women with the history of successful pregnancies were included in the study. The lymphocytes were isolated from peripheral blood and stained with monoclonal antibodies. The expression of selected surface molecules was estimated using the flow cytometric method. Results: We found that the percentage of T CD4+ lymphocytes, CD3-16/56+ cells, and T CD8+11b- cells was significantly higher in patients with recurrent pregnancy loss in comparison with healthy women. The peercentage of B-1 CD19+5+ lymphocytes was also significantly higher in women with unexplained habitual miscarriages in comparison with healthy women. Furthermore, we found higher expression of CD25 molecule on T CD3+ and T CD4+ lymphocytes in the study group, when compared to controls. Moreover, the percentages of B CD19+ and T suppressor CD8+11b+ lymphocytes were lower in women with pregnancy failures in comparison with the control group. The percentage of T CD3+ lymphocytes and T CD8+ cells did not differ in both studied groups. Similarly, the expression of CD25 antigen and HLA-DR molecule on T CD8+ did not differ in the study group, when compared to controls. Concvlusion: Our results can suggest that immunological alterations may be involved in the etiopathogenesis of unexplained recurrent pregnancy loss.

334/2523

Tytuł oryginału: Direct antimicrobial properties of substance P.
Autorzy: Kowalska Katarzyna, Carr Daniel B., Lipkowski Andrzej W.
Źródło: Life Sci. 2002: 71 (7) s.747-750, il., tab., buibliogr. 14 poz.
Sygnatura GBL: 304,948

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The structural similarity between substance P (SP, Arg-Pro-Lys-Pro-Gln-Gln-Phe-Gly-Leu-Met-NH2) and Arg/Pro rich bactericidal peptides suggests a possible direct effect of SP on invasive microbes. We now present evidence that substance P possesses direct antimicrobial activity, highest against S. aureus. A substance P antagonist allso possess such activity but while less potent than substance P agonist S. aureus, is more potent than substance P against C. albicans. Our data also show that the endogenous peptides bradykinin and neurotensin, that also play role in modulation of the host-defense system in situ, have antimicrobial properties but are less potent than substance P.

335/2523

Tytuł oryginału: New renin inhibitors with pseudodipeptidic units in P1-P1' and P2'-P3' positions.
Autorzy: Paruszewski Ryszard, Jaworski Paweł, Winiecka Iwona, Tautt Jadwiga, Dudkiewicz Jadwiga
Źródło: Chem. Pharm. Bull. (Tokyo) 2002: 50 (6) s.850-853, tab., bibliogr. 21 poz.
Sygnatura GBL: 304,601

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: A series of four new potential renin inhibitors has been synthesized. The structure of the compounds was designed in such a way as to produce agents resistant to enzymatic degradation, metabolically stable, possibly potent and with improved oral absorption. All positions of the 8-13 fragment of the human angiotensinogen were occupied by unnatural units (two unnatural amino acids in position P3 and P2 and two pseudodipeptides in positions P1-P1, and P2-P3,). Both N- and C-terminal functions of the inhibitors were blocked with tert-Boc and ethyl ester groups. Their hydrophobicity evaluated as a log P value, calculated by a computer method, was 6.57 and 6.08 respectively. All peptides were obtained by the carbodiimide method in solution and purified by chromatography on the SiO2 column. Their resistance to enzymatic degradation was assayed by determination of stability against chymotrypsin activity. The potency was measured in vitro by a spectrofluorimetric method (assay of Leu-Val-Tyr-Ser released from the N-acetyltreadecapeptide substrate by renin in the presence of the inhibitor). All inhibitors were stable to chymotrypsin. Their IC50 (M/I) values were: 9.6 x 10**-4 (12), 1.6 x 10**-5 (17), 1.0 X 10**-5 (22) and 1.0 x 10**-5 (23) respectively.

336/2523

Tytuł oryginału: Myocardial ischemia possibly mediated by cysteinyl leukotrienes.
Autorzy: Szczeklik Andrew, Niżankowska Ewa, Mastalerz Lucyna, Bochenek Grażyna
Źródło: J. Allergy Clin. Immunol. 2002: 109 (3) s.572-573, tab., bibliogr. 8 poz.
Sygnatura GBL: 301,408

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

337/2523

Tytuł oryginału: PRESTO (a mammoth trial that could have been prevented), HPS and REMATCH.
Autorzy: Doehner Wolfram, Ponikowski Piotr, Anker Stefan D.
Źródło: Int. J. Cardiol. 2002: 83 (2) s.99-102, bibliogr. 17 poz. - 74 Doroczne Spotkanie Towarzystwa Chorób Serca Anaheim 11. 2001
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

338/2523

Tytuł oryginału: Myocardial ischemia and autonomic activity in dippers and non-dippers with coronary artery disease: assessment of normotensive and hypertensive patients.
Autorzy: Kurpesa Małgorzata, Trzos Ewa, Drożdż Jarosław, Bednarkiewicz Zbigniew, Krzemińska-Pakuła Maria
Źródło: Int. J. Cardiol. 2002: 83 (2) s.133-142, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 305,693

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: The aim of this study was to assess the relations between the circadian variations of blood pressure (BP) and the pattern of ischemia and autonomic activity in normotensive and hypertensive patients with coronary artery disease (CAD). Patients and methods: On the basis of the results of ambulatory BP monitoring, 115 patients with stable CAD were divided into Group 1 (with arterial hypertension) and Group 2 (normotensives). Groups were subdivided into dippers and non-dippers. Holter monitoring was performed to assess the occurrence and circadian pattern of ischemic episodes. Time domain and frequency domain HRV analyses were performed to evaluate the autonomic activity. Results: The total number of ischemic episodes was similar in dippers and non-dippers. Non-dippers had a greater number of silent episodes and a different circadian pattern of ischemia with more night episodes. Among the time-domain HRV parametres, only SDNN was similar in dippers and non-dippers. Non-dippers had lower pNN50 and rMSSD - the parameters expressing parasympathetic activity. Differences between diurnal and nocturnal results of spectral HRV analysis were observed in dipper patients only. They presented an elevation of HF power and a decline of LF power at night. All differences between dippers and non-dippers were of similar significance in both hypertensives and normotensives. Conclusions: A lack of a nocturnal fall in BP is present in normotensive and hypertensive patients with CAD. Non-dippers with CAD had silent and nightime ischemia more often. They also had an abnormal pattern of autonomic activity with higher sympathetic and lower parasympathetic modulation.

339/2523

Tytuł oryginału: Serum levels of selected adhesion molecules in patients with coronary artery disease.
Autorzy: Mizia-Stec Katarzyna, Zahorska-Markiewicz Barbara, Mandecki Tadeusz, Janowska Joanna, Szulc Andrzej, Jastrzębska-Maj Ewa
Źródło: Int. J. Cardiol. 2002: 83 (2) s.143-150, tab., bibliogr. 32 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: Adhesion molecules have been suggested as mediators of atherosclerotic inflammatory process. They may contribute to the pathogenesis of stable and unstable angina. Methods: The study group consisted of 59 patients with coronary artery disease: 27 patients with stable exertional angina (group A), 32 patients with unstable angina (group B). 20 healthy persons acted as controls (group C). Serum levels of soluble Intercellular Adhesion Molecule 1 (sICAM-1), Vascular Cell Adhesion Molecule 1 (sVCAM-1), sE-selectin, sP-selectin were measured both before and after the treadmill ECG stress test in groups A and C. In group B the measurements were carried out at 6, 24, and 48 hours following an episode of chest pain. Results: There were no differences between the baseline serum levels of adhesion molecules as determined in groups A and C. In patients with stable angina, the post-exercise concentrations of sE-selectin were significantly higher (68.8 ń 29 ng/ml) in comparison to both baseline- (38.7 ń 15 ng/ml), and group C-values (pre-exercise: 35.1 ń 16; post-exercise: 49.9 ń 15 ng/ml). In unstable patients, serum sP-selectin (190.1 ń 99 ng/ml) and sVCAM-1 levels (1359 ń 299 ng/ml) were higher when compared to those found in groups A (142.3 ń 24; 962 ń352 ng/ml, respectively) and C (136.4 ń 33; 851 ń 168 ng/ml, respectively). Conclusions: Serum levels of soluble adhesion molecules in patients with stable angina are comparable to those of healthy persons. Stress test-induced increase of sE-selectin concentration may reflect endothelial response to exercise. Unstable angina is characterized by significant elevation of sP-selectin and sVCAM-1 serum levels which seems to be related to enhanced platelets and leukocytes activation.

340/2523

Tytuł oryginału: Atrial natriuretic peptide level after cardioversion of chronic atrial fibrillation.
Autorzy: Wozakowska-Kapłon Beata, Opolski Grzegorz
Źródło: Int. J. Cardiol. 2002: 83 (2) s.159-165, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Heart endocrine studies concerning patients with chronic atrial fibrillation (AF) have become increasingly important. Atrial natriuretic peptide (ANP) is released from atrial myocytes. The increased level of ANP in patients with AF is probably caused by the hemodynamic effect of the arrhythmia. The aim of this study was to explore plasma ANP levels in patients with chronic AF and to describe plasma ANP concentration changes following sinus rhythm (SR) restoration. The study group was comprised of 42 patients, aged between 43 and 76 years with chronic ASF (more than 1 month) and a relatively controlled ventricular response (85.8 ń 11.3 beats/min). Plasma ANP levels were measured before and 24 h after AF cardioversion. The control group comprised of 11 subjects. All had normal SR without history of AF and were compatible in age, sex and concomitant diseases with the examined group. ANP level values were expressed as mean ń standard deviation. The mean plasma ANP level in the AF group was significantly higher than in the control group (59.5 ń 16.6 vs. 34.3 ń 10.2 pg/ml, P 0,001). Electrical or pharmacological cardioversion was performed in 42 patients. SR was succesfully restored in 35 patients. Plasma ANP concentrations decreased significantly from baseline values (from 59.4 ń 16.6 to 31.4 ń 15.0 pg/ml, P 0.001) 24 h after cardioversion in the successful group, while they remained unchanged (60.2 ń 10.7 to 59.4 ń 10.4 pg/ml, NS) in patients with an unsuccessful cardioversion. Conclusion: The mean concentration of ANP in patients with chronic AF was nearly two-times higher than in the control group with sinus rhythm. Conversion to SR was associated with a significant decrease and normalization in plasma ANP concentrations.

341/2523

Tytuł oryginału: Inhibitory effect of acetylsalicylic acid on metalloproteinase activity in human lung adenocarcinoma at different stages of differentiation.
Autorzy: Karna Ewa, Pałka Jerzy A.
Źródło: Eur. J. Pharmacol. 2002: 443 (1/3) s.1-6, il., tab., bibliogr. s. 5-6
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The mechanism underlying the anticancer effect of nonsteroidal anti-inflammatory drugs (NSAIDs) is not clear. We adressed the question whether the alterations in collagen content in lung adenocarcinomas reported in previous studies result from dysregulation of gelationylytic activity and whether the activity is altered by acetylsalicylic acid in vitro. Human lung adenocarcinomas were divided into three groups: well-differentiated (G1), moderately differentiated (G2) and poorly differentiated (G3) tumors. Each group was compared with normal lung tissue with respect to tissue collagean and collagen degradation product content (hydroxyproline assay), gelatinolytic activity (zymography) and the expression of matrix metalloproteinases, MMP-2 and MMP-9 (Western immunoblot). Moreover, in the studied tissues, the effect of acetylsalicylic acaid on gelatinolytic activity was measured. The lung adenocarcinoma G1 had a similar collagen content as normal lung tissue but increased amounts of collagen degradation products and free hydroxyproline. These phenomena were accompanied by a marked increase in gelatinolytic activity (NMP-2 and MMP-9) in the G1 tumor. In adenocarcinoma G2, the free hydroxyproline content and gelatinolytic activity were increased, while the collagen and collagen degradation product contents were not markedly altered, compared to control. In contrast, adenocarcinoma G3 had an increased tissue collagen content (by about 60 p.c.), decreased percentage of collagen degradation products and similar gelatinolytic activity, compared to normal lung. Acetylsalicylic acid...

342/2523

Tytuł oryginału: Imipramine but not 5-HT1A receptor agonists or neuroleptics induces adaptive changes in hippocampal 5-HT1A and 5-HT4 receptors.
Autorzy: Zahorodna Agnieszka, Tokarski Krzysztof, Bijak Maria
Źródło: Eur. J. Pharmacol. 2002: 443 (1/3) s.51-57, il., bibliogr. s. 56-57
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: It has been reported that the treatment with a tricyclic antidepressant imipramine induces an increase in the sensitivity of 5-HT1A receptors and a decrease in the sensitivity of 5-HT4 receptors in the rat hippocampus. 5-HT1A receptor agonists and neuroleptics also affect 5-HT1A receptors in different brain areas; therefore, it was of interest to compare their effects on hippocampal 5-HT receptors with the influence of the well-established antidepressant imipramine. We studied the effects of repeated treatment with imipramine, the 5-HT1A receptor agonists 8-hydroxy-2(di-n-propylamino)tetralin (8-OH-DPAT) and buspirone, and the neuroleptics haloperidol and clozapine on the sensitivity of rat hippocampal CA1 neurons to 5-HT1A - and 5-HT4 receptor activation. Imipramine was administered for 21 days (10 mg/kg p.o., twice daily), 8-OH-DPAT for 7 days (1 mg/kg s.c., twice daily) and buspirone for 21 days (5 mg/kg s.c., twice daily). The rats received haloperidol (1 mg/kg) and clozapine (30 mg/kg) for 6 weeks in drinking water. Hippocampal slices were prepared 2 days after the last treatment with imipramien, 8-OH-DPAT or buspirone, and 5 days after the last treatment with the neuroleptics. Using an extracelllar in vitro recording, we studied changes in the amplitude of stimulation-evoked population spikes, induced by 5-HT, 8-OH-DPAT and the 5-HT4 receptor agonist zacopride. Activation of 5-HT1A receptors decreased, while activation of 5-HT4 receptors increased the amplitude of population spikes. Imipramine significatly enhanced the inhibitory effects of 5-HT and 8-OH-DPAT, and attenuated...

343/2523

Tytuł oryginału: Circulating E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in men with coronary artery disease assessed by angiography and disturbances of carbohydrate metabolism.
Autorzy: Kowalska Irina, Strączkowski Marek, Szelachowska Małgorzata, Kinalska Ida, Prokop Jolanta, Bachórzewska-Gajewska Hanna, Stępień Agnieszka
Źródło: Metabolism 2002: 51 (6) s.733-736, tab., bibliogr. 22 poz.
Sygnatura GBL: 303,583

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: It is hypothesized that adhesion molecules could be an early predictor of coronary artery diseases. Therefore we investigated the relationship between the concentrations of soluble forms of adhesion molecules and disturbances of glucose metabololism in 78 men referred for coronary angiography but with no previous history of diabetes. The group consisted of 78 men (mean age, 47.6 ń 7.0 years; mean body mass index [BMI], 28.4 ń 3.24 with the symptoms of angina pectoris and positive exercise test. All subjects were given a standard oral glucose tolerance test (OGTT) with glucose and insulin estimations. Fasting plasma concentrations of the soluble (s) forms of E-selectin, intercelular adhesion cell molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), and HbA1c were also measured. According to the OGTT, 10.2 p.c. of the patients (n = 8) fulfilled the criteria for type 2 diabetes mellitus and 44.9 p.c. (n = 35) for impaired glucose tolerance (IGT). The highest concentrations of sE-selectin were observed in patients with type 2 diabetes mellitus and were significantly higher in comparison to the group with normal glucose tolerance and IGT. The concentration of sVCAM-1 increased with the progression of disturbances of glucose metabolism and remained the highest in type 2 diabetic patients. sICAM-1 concentration was not significantly different. sE-selectin concentration correlated significantly with fasting glucose (r = 0.23, P = .041), postload glucose (r = 0.39, P = .001), and postload...

344/2523

Tytuł oryginału: Increased rate of cholesterologenesis - a possible cause of hypercholesterolemia in experimental chronic renal failure in rats.
Autorzy: Szołkiewicz M[arek], Sucajtys E., Chmielewski M., Wołyniec W., Rutkowski P., Bogusławski W., Świerczyński J., Rutkowski B[olesław]
Źródło: Horm. Metab. Res. 2002: 34 (5) s.234-237, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 312,226

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Hipercholesterolemia plays an important role in the lipid abnormalities in chronic renal failure (CRF). It is thourght to contribute to both a progression of renal failure and atherosclerosis. Despite intensive research, the etiopathogenesis of hypercholesterolemia in CRF patients is still obscure. The present sudy was designed to evaluate the possible role of cholsterol overproduction in the development of hypercholesterolemia associated with experimental CRF. We found that plasma total cholesterol and cholesterol distributed in VLDL, LDL and HDL concentrations were significantly enhanced in CRF rats. Simultaneously, the rate of liver cholesterol biosynthesis in vivo (measured by determining the incorporation of tritium from tritiated water intraperiotoneally injected into cholesterol), liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity and liver HMG-CoA reductase mRNA presence were elevated. Significant increases in activity of liver malic enzyme, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase NADPH-producing enzyme (required for cholesterol synthesis) have also been observed in CRF rats. In conclusion, the increased rate of liver cholesterol biosynthesis due to increase of HMG-CoA reductase and NADPH-producing enzyme gene expression could be one of the possible causes of hypercholesterolemia in CRF animals.

345/2523

Tytuł oryginału: Knockdown of spinal opioid receptors by antisense targeting á-arrestin reduces morphine tolerance and allodynia in rat.
Autorzy: Przewłocka Barbara, Sieja Agnieszka, Starowicz Katarzyna, Maj Marcin, Bilecki Wiktor, Przewłocki Ryszard
Źródło: Neurosci. Lett. 2002: 325 (2) s.107-110, il., bibliogr. 13 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The development of morphine tolerance and sciatic nerve injury-induced allodynia after functional knockdown of spinal opioid receptors using antisense oligonucleotides targetin á-arrestin was investigated. Ineffectiveness of morphine in neuropathic pain suggests an implication of the same mechanism in these two processes. The development of morphine tolerance (10 ćg intrathecally (i.th.), every 12 h) was significantly inhibited in rats, which received i.th. á-arrestin antisenses (2 nM). Acute and chronic (6 days) i.th. administration of antisenses antagonized the allodynia in the rat model of neuropathic pain. Our results demonstrated that i.th. administration of á-arrestin antisenses delayed development of tolerance to morphine and nerve injury-induced cold allodynia, which suggest that both of the investigated phenomena may be mediated by a similar mechanism, e.g. receptor desensitization. Moreover, the antisense oligonucleotides targeting á-arrestin may constitute a new approach to the therapy of neuropathic pain.

346/2523

Tytuł oryginału: Applications of derivative UV spectrophotometry for the determinations of cetirizine dihydrochloride in pharmaceutical preparations.
Autorzy: Drozd Joanna, Hopkała Hanna, Misztal Genowefa, Paw Beata
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.3-7, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A method for the determination of cetirizine dihydrochloride in pharmaceuticals by first-, second-, third- and fourth- order derivative spectrophotometry is described, using "peak" (P-P), and "peak - zero" (P-0) measurements. The calibration curves are linear within the concentration range of 7.5 - 22.5 ćg ml-1 for cetrizine dihydrochloride. The procedure is simple, rapid and the results are reliable.

347/2523

Tytuł oryginału: Determination of active substances in antiallergic and antiphlogistic multicomponent preparations by high performance liquid chromatography (HPLC).
Autorzy: Białecka Wanda, Kulik Anna, Kaniewska Teresa
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.9-14, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The aim of the present study was elaboration of an HPLC method enabling the identification and deterrmination of the content of selected compounds occurring in multicomponent preparations applied in allergic and non-allergic diseases of upper respiratory tracts. These compounds include: buzepide methyl iodide, clocinizine dihydrochloride, phenylpropanolamine hydrochloride, pseudophedrine sulfate and dexbrompheniramine maleate. The elaborated HPLC method shows that a good separation of the mentioned compounds is feasible. The regression analysis has demonstrated lineary of the method in concentration range suitable for the intended experiments. The determination of the compounds in pharmaceutical preparation and the statistical evaluation of the results indicate that both selectivity and precision of the method are good.

348/2523

Tytuł oryginału: Determination of the active ingredient loperamide hydrochloride in pharmaceutical caplets by high performance thin layer chromatography with ultraviolet absorption densitometry of fluorescence quenched zones.
Autorzy: Ruddy Daniel A., Sherma Joseph
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.15-18, tab., bibliogr. 11 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A gantitative method using silica gel HPTLC plates with fluorescent indicator, automated sample application, and automated UV absorption densitometry of the fluorescence quenching zones was developed and validated for determination of loperamide hydrochloride in anti-diarrheal medications. Samples of three brands of caplets assayed within 96.0 - 105 p.c. of the 2 mg label value. Repeatability was 3.3 p.c., 1.6 p.c. and 2.8 p.c. (RSD) for replicate analyses (n = 6) of three tablets. The errors of a blank-spike and standard analyses performed to evaluate accuracy were 2.00 p.c. and 2.02 p.c., respectively. The method is suitable application in a drug manufacturing quality control or regulatory analysis laboratory.

349/2523

Tytuł oryginału: The stability of oxymetazoline hydrochloride in aqueous solution.
Autorzy: Stanisz Beata
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.19-23, il., tab., bibliogr. 9 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The kinetics of the hydrolysis reaction of oxymetazoline hydrochloride in aqueous solution at three temperaturtes (343 K, 353 K, 363 K), over the pH-range 0.5 - 12.5 and ionic strenght 0.5 has been investgated. The changes of concentration of oxymetazoline hydrochloride were followed by the HPLC method with UV detection. In the pH range from 0.45 to 12.50, the hydrolysis of oxymetazoline consist of hydrolysis of oxymetazoline molecules catalyzed by hydrogen ions, spontaneous hydrolysis of the dissociated and undissociated oxymetazoline molecules. A minimal rate of the hydrolysis oxymetazoline was observed to occur in the pH range from 2.0 to 5.0. Thermodynamic parameters of the reaction: energy, entropy and enthalpy of activation and the frequency factor for the specific rate constans were determined.

350/2523

Tytuł oryginału: Kinetics of talampicillin decomposition in solutions.
Autorzy: Pawełczyk Ewaryst, Płotkowiak Zyta, Helska Maria
Źródło: Acta Pol. Pharm. 2002: 59 (1) s.25-29, tab., bibliogr. 9 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Talampicillin stability in aqueous solutions was studied in a broad range of pH values using as medium solutions of hydrochloric acid (pH 0.4 - 1.8), phosphate buffers (pH 2.05 - 3.13 and 6.03 - 8.04), acetate buffer (pH 3.87-5.28) and borate buffer (pH 8.90 - 9.10) as well as sodium hydroxide solution (pH 11.48). For the determination of talampicilin concentration changes in kinetic studies, two methods were used: iodometric and spectrophotometric in UV(lambdamax = 254.5 nm). The catalytic velocity constans (kh+, kx, ko) were established, the log k-pH profile (35řC) was found, thermodynamic parameters were calculated of the hydrolisis reaction of the á-lactam bond (kH+: EA=67.9 kJ mol**-1, Delta S"= -92.4 JK**-1 mol-1, Delta G"=92.6 kJ mol**-1; kx:EA = 31.8 kJ mol**-1, Delta S" = 347.1 JK**-1 mol**-1, Delta G" =131.1 kJ mol**-1...

351/2523

Tytuł oryginału: A modified neutral comet assay: elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody.
Autorzy: Wojewódzka Maria, Buraczewska Iwona, Kruszewski Marcin
Źródło: Mutat. Res 2002: 518 (1) s.9-20, il., bibliogr. 43 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Comet assay under neutral conditions allows the detection of DNA double-strand breaks (DSB), considered to be the biologically relevant radiation-induced lesion. In this report, we describe modifications of the neutral comet method, which simplify and facilitate its use for estimation of DNA DSB in X-irradiation mammalian cells in centure. The analysis carried out according to this protocol takes less time than those most often applied. Also, the use of lysis at 50ř C is avoided; this is important in view of the presence of heat-labile sites in the chromatin of irradiated cells, recently reported by Rydberg [Radiation-induced heat-labile sites that convert into DNA double-strtand breaks, Radsaiation Research 153 (2000) 805-812]. The comets have well-defined, sharp limits, suitable for image analysis. The chromatin of the hydrogen peroxide-treated or UV-C-irradiated cell remains condensed similarly to that of the control cells. We checked the neutral comets for the presence of single-stranded DNA by means of a specific antibody. The results point to a satisfactory sensitivity of the modified neutral comet assay and its specificity for DSB. The minimum detection level of the modified neutral comet assay is about 5 Gy.

352/2523

Tytuł oryginału: Countrywide spread of CTX-M-3 extended-spectrum á-lactamase-producing microorganisms of the family Enterobacteriaceae in Poland.
Autorzy: Baraniak Anna, Fiett Janusz, Sulikowska Agnieszka, Hryniewicz Waleria, Gniadkowski Marek
Źródło: Antimicrob. Agents Chemother. 2002: 46 (1) s.151-159, tab., bibliogr. 42 poz.
Sygnatura GBL: 305,849

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Eighty-four clinical isolates of the family Enterobacteriaceae from 1998 to 2000 in 15 hospitals in 10 polish cites, were analyzed. All the isolates produced á-lactamases with pIs of 8.4 and 5.4, and the pI 8.4 enzymes were demonstrated to hydrolyze cefotaxime but not ceftazidime in the in vitro bioassay. PCR analysis and DNA sequencing have revealed that in all cases the pI 8.4 á-lactamase was probably the CTX-M-3 extended-spectrum á-lactamase (ESBL) variant, which was originally identified in 1996 in Praski Hospital in Warsaw. In the majority of isolates, blaCTX-M-3 genes resided large conjugative plasmids with similar fingerprints, which, in the contex of the high degree of diversity of the randomly amplified polymorphic DNA types of the isolates, suggest that horizontal transfer of plasmids was likely the main mechanism of CTX-M-3 spread. The dissemination of plasmids was probably preceded by the center-to-center transmission of several strains, as indicated by the identification by pulsed-field gel electrophoresis of closely related or possibly related Klebsiella pneumoniae, Eschrichia coli, and Citrobacter freundii isolates in five different hospitals. CTX-M-3-producing organisms revealed a very high degree of diversity in á-lactam resistance levels and patterns. This was attributed to several factors, such as the production of other á-lactamases including additional ESBLs, possible quantitative variations in CTX-M-3 expression, segregation of AmpC derepressed mutants, and permeability alterations.

353/2523

Tytuł oryginału: A randomized, double-blind trial of the effect of treatment with montelukast on bronchial hyperresponsiveness and serum eosinophilic cationic protein (ECP), soluble interleukin 2 receptor (sIL-2R), IL-4, and soluble intercellular adhesion molecule 1 (sICAM-1) in children with asthma.
Autorzy: Stelmach Iwona, Jerzyńska Joanna, Kuna Piotr
Źródło: J. Allergy Clin. Immunol. 2002: 109 (2) s.257-263, il., tab., bibliogr. 43 poz.
Sygnatura GBL: 301,408

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: Background: Anti-inflammatory properties of leukotriene modifiers and their on bronchial hyperresponsiveness have npt been studied in children with asthma. Objective: The primary objective of this study was to determine the changes in serum levels of inflammatory mediators, clinical efficacy, and bronchial hyperresponsiveness after treatment with montelukast. Methods: In this double-blind, randomized, placebo-controlled trial, 39 children with mild-to-moderate atopic asthma were randomly allocated to receive montelukast or placebo or 6 weeks. Main outcome measures were changes in serum concentrations of soluble interleukin 2 receptor (sIL-2R), IL-4, and soluble intercellular adhesion molecule 1 (sICAM-1); peripheral blood eosinophil count; and eosinophilic cationic protein (ECP). Asthma severity score, FEV1, and bronchial hyperreactivity (BHR) for histamine were secondary end points. Results: Compared to placebo, serum concentrations of IL-4 sICAM-1, and ECP and eosinophil blood counts significantly decreased after 6 weeks of treatment with montelukast. Montelukast significantly improved asthma control and FEV1. Montelukast resulted in within-group significant decrease in levels of serum sIL-2R (611 vs 483 pg/mL), IL-4 (0.123 vs 0.102 pg/mL), sICAM-1 (280 vs 244 ng/mL), and ECP (74 vs 59 ćg/mL) and in eosinophil blood counts (349 vs 310 cells/mm3). Mean FEV1 value changed from 85 p.c. of predicted to 95 p.c. (P .001) and for histamine (PC20H) from 2.8 mg/L to 3.8 mg/mL (P .001) after treatment with montelukast...

354/2523

Tytuł oryginału: Lovastatin potentiates antitumor activity of doxorubicin in murine melanoma via an apoptosis-dependent mechanism.
Autorzy: Feleszko Wojciech, Młynarczuk Izabela, Olszewska Dominika, Jalili Ahmad, Grzela Tomasz, Lasek Witold, Hoser Grażyna, Korczak-Kowalska Grażyna, Jakóbisiak Marek
Źródło: Int. J. Cancer 2002: 100 (1) s.111-118, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 305,029

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Lovastatin, a drug successfully used in the clinic to prevent and to treat coronary heart disease, has recently been reported to decrease the incidence of melanoma in lovastatintreated patients. Lovastatin has also been proved to potentiate antitumor effects of both cisplatin and TNF-ŕ in murine melanoma models. Recently, an augmented therapeutic effect of lovastatin and doxorubicin has been reported in 3 tumor models in mice. In our preliminary study lovastatin caused retardation of melanoma growth in mice treated with doxorubicin (Feleszko et al. J. Nati Cancer Inst 1998;90:247-8). In the present report, we supplement our preliminary observations and demonstrate in 2 murine and 2 human melanoma cell lines that lovastatin effectively potentiates the cytostatic/cytotoxic activity of doxorubicin in vitro via an augmentation of apoptosis (estimated with PARP-cleavage assay, annexin V assay and TUNEL). The combined antiproliferative activity of lovastatin and doxorubicin was evaluated using the combination index (CI) method of Chou and Talalay, revealing synergistic interactions in melanoma cells exposed to lovastatin and doxorubicin. In BI6FI0 murine melanoma model in vivo, we have demonstrate significantly increased sensitivity to the combined treatment with both lovastatin (5 mg/kg for 14 days) and doxorubicin (4 x I mg/kg) as compared with either agent acting alone. Lovastatin treatment resulted also in significant reduction of the number of experimental metastasis in doxorubicin-treated mice The results of our studies suggest that lovastatin may enhance the effectiveness of chemotherapeutic agents in the treatment of malignant melanomas.

355/2523

Tytuł oryginału: Adsorption characteristics of human plasma fibronectin in relationship to cell adhesion.
Autorzy: Kowalczyńska Hanna M., Nowak-Wyrzykowska Małgorzata, Dobkowski Jacek, Kołos Robert, Kamiński Jarosław, Makowska-Cynka Alicja, Marciniak Ewa
Źródło: J. Biomed. Mater. Res. 2002: 61 (2) s.260-269, il., tab., bibliogr. 57 poz.
Sygnatura GBL: 305,818

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Adsorption of human plasma fibronectin (FN) on nonsulfonated and sulfonated polymer surfaces was studied, by using a polyclonal antiserum to FN and the ELISA method. ELISA signal was recorded as a function of FN concentration in solutions. The concentration dependence of FN binding shows the saturation effect in the range 5-10 ćg/mL. ELISA data are discussed in the terms of a self-assembled monolayer and different conformations of the FN molecule. The early adhesion of L1210 cells to polymer surfaces after prior adsorption of FN on these surfaces was studied under static conditions. In the case of FN adsorbed on sulfonated surfaces, the relative number of adhering cells increased with the increase of the interfacial surface tension (i.e., the cell adhesion depends on the surface density of sulfonic groups). However, in the case of FN adsorbed on nonsulfonated surfaces, the relative number of adhering cells was low and independent on the interfacial surface tension. The ŕ5á1-integrin blocking by a monoclonal antibody resulted on sulfonated polymer surfaces. This indicates that cell adhesion to FN adsorbed on these surfaces is mostly mediated by the ŕ5á1-integrin. In contrast, in the case of FN adsorbed on nonsulfonated surfaces the cell adhesion was not inhibited by the ŕ5á1-integrin blocking.

356/2523

Tytuł oryginału: Antagonism of picrotoxin-induced changes in dopamine and serotonin metabolism by allopregnanolone and midazolam.
Autorzy: Maciejak Piotr, Krząścik Paweł, Członkowska Agnieszka I., Szyndler Janusz, Bidziński Andrzej, Walkowiak Jerzy, Kostowski Wojciech, Plaznik Adam
Źródło: Pharmacol. Biochem. Behav. 2002: 72 (4) s.987-991, il., tab., bibliogr. [11] poz.
Sygnatura GBL: 312,498

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

myszy

Streszczenie angielskie: The effects of allopregnanolone and midazolam, given intracerebroventricularly, on the behavioral and biochemical effects of picrotoxin, were examined in a model of neurotoxin-induced seizures, in mice. After acute injections, midazolam (ED50 = 39.8 nmol) and allopregnanolone (ED50 = 11.0 nmol) produced similar and dose-dependent protection against picrotoxin-induced seizures. Picrotoxin given intraperitoneally at the ED85 dose decreased significantly the concentration of serotonin (5-HT), dopamine (DA), homovanilic acid (HVA) and 3,4-dihydroxyindolacetic acid (DOPAC), in the mouse striatum and the frontal cortex, in the period of time immediately preceding the onset of seizures. A single injection of allopregnanolone more potently, in comparison to midazolam, antagonized the biochemical action of picrotoxin, abolishing its effects on DA, HVA and 5-HT concentration, in the mouse striatum and the frontal cortex. These results for the first time provide a direct argument for an involvement of central dopaminergic and serotonergic systems in the seizure development. The present data add also to the accumulating evidence suggesting a favorable pharmacological profile for some neurosteroids currently considered to have a future role in the management of epilepsy.

357/2523

Tytuł oryginału: The influence of luteinizing hormone-releasing hormone analog on serum leptin and body composition in women with solitary uterine myoma.
Autorzy: Nowicki Michał, Adamkiewicz Grażyna, Bryc Władysław, Kokot Franciszek
Źródło: Am. J. Obstet. Gynecol. 2002: 186 (3) s.340-344, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 300,265

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: The purpose of this study was to examine the influence of luteinizing hormone-releasing hormone analog goserelin on serum leptin and body composition in women with solitary uterine myoma. Study design: Fifteen women who were regularly menstruating and not obese were included. In all subjects, serum concentrations of leptin, insulin, testosterone, progesterone, and estradiol and body mass index and waist-to-hip ratio were measured before and after 4, 8, and 12 weeks of treatment with goserelin (3.6 mg every 4 weeks). Fat mass and lean body mass were measured by dual energy radiographic densitometry at baseline and after 12 weeks of therapy. Data were analyzed by multiple way analysis of variance and both simple and multiple regression. Results: The treatment caused a significant regression of myoma. Body weight, fat, ane lean mass were unchanged. No changes in plasma leptin (even after correction for fat mass) were noted during the treatment. Plasma estradiol decreased below castrate levels. Plasma progesterone decreased significantly, and testosterone tended to decline during the study. At baseline a highly significant positive correlation was found between serum leptin and fat mass. In a multiple regression analysis, neither the change in fat mass nor any of the hormonal parameters explained the significant portion of variance of plasma leptin during the treatment. Conclusion: Pharmacologic gonadectomy does not influence plasma leptin concentrations in women if body fat mass is unchanged.

358/2523

Tytuł oryginału: Journal of Pharmacy and Pharmacology
Tytuł polski: Pharmacological properties and SAR of new 1,4-disubstituted piperazine derivatives with hypnotic-sedative activity.
Autorzy: Chilmonczyk Zdzisław, Mazgajska Maria, Iskra-Jopa Joanna, Chojnacka-Wójcik Ewa, Tatarczyńska Ewa, Kłodzińska Aleksandra, Nowak Jerzy Z.
Źródło: J. Pharm. Pharmacol. 2002: 54 (5) s.689-698, il., tab., bibliogr. s. 696-698
Sygnatura GBL: 300,961

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Preparation, pharmacological properties and structure-activity relationships of new pyrimidylpiperazine derivatives, exhibiting sedative and hypnotic activity in mice, are reported. The hypnotic activity of the compounds was comparable with that of zopiclone (the known hypnotic-sedative agent), their interaction with ethanol, however, being much lower. The obtained results suggested that zopiclone and pyrimidylpiperazines 2, 4 and 5 exerted their pharmacological activity through a different mechanism - zopiclone through the interaction with benzodiazepine receptors and compounds 2, 4 and 5 through an unidentified molecular target. The pharmacological properties of compound 3 could be the result of a mixed mechanism of action, combining the properties of zopiclone and those of compounds 2, 4 and 5. A common feature of zopiclone and compounds 2 and 3 was that, after their systemic administration, independently of mechanism of action, together with the hypnotic effect a reduction of the 5-HT turnover in the mouse brain was observed. Minimum structural requirements for the hypnotic activity were formulated. Structural considerations have shown that removing the ŕ-carbonyl group did not influence the drug's ability to inhibit the locomotor activity. However, it did influence its ability to disturb motor coordination or abolish the righting reflex within non-lethal doses.

359/2523

Tytuł oryginału: Is diminished pubocervical fascia collagen content a risk factor for failure of surgical management of genuine stress urinary incontinence in women?
Autorzy: Skorupski Paweł, Rechberger Tomasz, Postawski Krzysztof, Woessner jr J. Frederick, Jakowicki Jerzy A.
Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 102 (2) s.195-198, tab., bibliogr. 19 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: The assessment of relationship between pubocervical collagen content and clinical results of surgical treatment of genuine stress urinary incontinence (GSUI) in women. Methods: Twenty-four women treated for genuine stress urinary inontinence were included into the study. All women underwent the same surgical procedure. The samples of pubocervical fasia were taken at the time of surgery. The contents of acid soluble, pepsin soluble, insoluble fraction of collagen, total collagen and collagen crosslinks were measured. The study of pubocervical fascia collagen metabolism included also estimation of collagen activity. At follow-up done 5 years following surgery, 20 patients reported symptoms of GSUI (study group). Four women were still without symptoms of urine leakage (control group). The biochemical parameters of pubocervical fascia did not show, statistically significant differences between cimpared groups. Conclusions: The pubocervical fascia collagen metabolism does not have impact on the results of anti-incontinence surgery.

360/2523

Tytuł oryginału: Homozygous combination of calpain 10 gene haplotypes is associated with type 2 diabetes mellitus in a Polish population.
Autorzy: Malecki Maciej T., Moczulski Dariusz K., Klupa Tomasz, Wanic Krzysztof, Cyganek Katarzyna, Frey Jakub, Sieradzki Jacek
Źródło: Eur. J. Endocrinol. 2002: 146 (5) s.695-699, tab., bibliogr. 19 poz.
Sygnatura GBL: 301,344

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: The polymorphisms of two genes have recently been associated with complex forms of type 2 diabetes mellitus (T2DM): calpain 10 and peroxisome proliferator-actvated receptor-ç (PPARç). Calpain 10 is a member of a large family of intracellular protease. It was shown in Mexican-Americans and other populations that variants of three single nucleotide polymorphisms (SNPs), -43, -19, and -63, of this ubiquitously expressed protein influence susceptibility to T2DM. However, substantial differences were shown between ethnic groups in at risk alleles and haplotypes as well as in their attributable risk. Thus, it is important to determine the role of calpain 10 in various populations. Aim: To examine the role of calpain 10 SNPs -43, -19, and -63 in genetic susceptibility to T2DM in a Polish population. Methods: Overall, 377 individuals were examined: 229 T2DM patients and 148 control individuals. The groups were genotyped for calpain 10 SNP-43, SNP-19, and SNP-63. SNP-19 was examined by electrophoresis of the PCR product on agarose gel by size, while the restriction fragment length polymorphism (RFLP) method was used for the two other markers. Difference in allele, genotype, haplotype, and haplotype combination distribution between the groups were examined by Xý test. Results: Distributions of alleles, genotypes, and haplotypes at three loci defined by examined SNPs were not significantly different between the groups. However, the homozygote combination of 121 haplotype was more prevalent in the T2DM group than in the controls (17.9 p.c. vs 10.1 p.c., P = 0.039)...

361/2523

Tytuł oryginału: Prognostic value of low-dose dobutamine echocardiography in patients with idiopathic dilated cardiomyopathy.
Autorzy: Drożdż Jarosław, Krzemińska-Pakuła Maria, Plewka Michał, Ciesielczyk Michał, Kasprzak Jarosław D.
Źródło: Chest 2002: 121 (4) s.1216-1222, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 300,494

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Study objectives: Dobutamine echocardiography is widely used for the evaluation of myocardial contractile reserve. The purpose of the study was to determine the prognostic value of low-dose dobutamine echocardiography in patients with idiopathic dilated cardiomyopathy (IDCM). Patients: The study group consisted of 77 consecutive patients with recently diagnosed IDCM (mean [ń SD] age, 49 ń 9 years; men, 82 p.c.) and left ventricular (LV) ejection fractions of 40 p.c. Interventions: Two-dimensional and Doppler echocardiographic variables were measured before and after the infusion of dobutamine at the rate of 10 ćg/kg/min for 5 min. Measurements and results: During a mean follow-up period of 63 ń 7 months (range, 49 to 75 months) 30 patients (39 p.c.) died and five patients (6 p.c.) underwent successful heart transplantations. Using multivariate regression analysis, the only significant factors related to fatal outcome or the need for cardiac transpantation were the following: (1) LV end-systolic volume of 150 mL after low-dose dobutamine infusion (odds ratio [OR], 2.2; confidence interval [CI], 1.2 to 4.1; p = 0.011); (2) no decrease of LV end-diastolic volume after dobutamine infusion (OR, 1.9; CI, 1.1 to 3.4; p = 0.031); (3) atrial fibrillation (OR, 2.7; CI, 1.4 to 5.3; p = 0.003); and (4) male gender (OR, 2.6; CI, 1.2 to 5.5; p = 0.017). A scoring system was proposed with one point assigned for each of the above-mentioned factors. The mortality rates for total scores of 0, 1, 2, 3, and 4 were 0 p.c., 19 p.c., 48 p.c., 83 p.c., and 100 p.c., respectively. Conclusion: The response of the LV to low-dose dobutamine infusion adds clinically valuable prognostic information to the evaluation of the patient with IDCM.

362/2523

Tytuł oryginału: Stimulatory effects of pituitary adenylate cyclase-activating polypeptide on inositol phosphates accumulation in avian cerebral cortex and hypothalamus.
Autorzy: Nowak Jerzy Z., Pigulowska Aleksandra, Kuba Katarzyna, Zawilska Jolanta B.
Źródło: Neurosci. Lett. 2002: 323 (3) s.179-182, il., bibliogr. 22 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ptaki

Streszczenie angielskie: This study has demonstrated that the short and long form of the pituitary adenylate cyclase-activating polypeptide (PACAP), i.e. PACAP27 and PACAP38, moderately but significantly, and in a concenration (0.5-5 ćM)-dependent manner, stimulated inositol phosphates (IPs) accumulation in myo-[3H]inositol-prelabeled cerebral cortical and hypothalamal slices of chick and duck, and in slices of rat cerebral cortex; both peptides had no effect on IPs formation in rat hypothalamus. Vasoactive intestinal peptide (VIP; 0.5-5 ćM) weakly enhanced IPs accumulation in chick hypothalamus, had no significant action in chick cerebral cortex (in fact there was a tendency to attenuate the IPs response in this tissue), and slighty, but significantly, inhibite the IPs accumulation in rat cerebral cortex. VIP showed no activity in rat hypothalamus. It is cincluded that tha stimulatory action of PACAP on phosphoinositide metabolism in avian cerebral cortex, similar to rat cerebral cortex, is mediated via phospholipase C-linked PAC1 type receptors. In chick hypothalamus, however, there may be a component of VPAC type receptors stimulating IPs formation.

363/2523

Tytuł oryginału: Long-term therapy with recombinant human erythropoietin decreases percentage of CD152+ lymphocytes in primary glomerulonephritis haemodialysis patients.
Autorzy: Trzonkowski Piotr, Myśliwska Jolanta, Dębska-Ślizień Alicja, Bryl Ewa, Rachoń Dominik, Myśliwski Andrzej, Rutkowski Bolesław
Źródło: Nephrol. Dial. Transplant. 2002: 17 (6) s.1070-1080, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 312,872

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Bacground. Recombinant human erythropoietin (rHuEpo) may affect the human immune system. The aim of the study was to examine changes in CD4+ and CD8+ T-cell subpopulations, the expression of the inhibitory molecule, CD152 on T lymphocytes and the levels of interleukins (IL), 2, 6, 10, 12 and tumour necrosis factor ŕ )TNFŕ) in primary glomerulonephritis chronic haemodialysis (HD) patients before and under rHuEpo treatment. Methods. Expression of T-cell surface molecules was measured in 14 HD patients ex vivo by flow cytometry of lymphocytes sampled from peripheral blood and in viutro using whole blood cell cultures stimulated either with phytohaemagglutinin (PHA) or with physiological as well as non-physiological does or rHuEpo. The concenrations of the cytokines were measured in the supernatans from non-or PHA-stimulated cultures using bioassays (IL2, IL6, TNFŕ) or ELISA test (IL10, IL12). Results. Compared with findings before the start of rHuEpotherapy the CD4+/CD8+ ratio increased after 1 year of follow-up, whereas the percentage of CD152+ peripheral blood lymphocytes decreased. The increase of the CD4+/CD8+ rtio was dependent on a decrease of the percentage of CD8+ cells. The decrease of CD152+ population affected mainly CD8+ CD152+ cells. All these effects became apparent after 6 months of rHuEpo treatment. In vitro stimulation of whole blood cultures revealed that the addition of PHA up-regulated the percentage of CD152+ lymphocytes, while physiological concentrations of rHuEpo decreased the percentage of CD8+ 152+ T cells...

364/2523

Tytuł oryginału: Carp erythrocyte lipids as a potential target for the toxic action of zinc ions.
Autorzy: Gabryelak Teresa, Akahori Anna, Przybylska Maria, Jóźwiak Zofia, Brichon Gerard
Źródło: Toxicol. Lett. 2002: 132 (1) s.57-64, il., tab., bibliogr. s. 63-64
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The effects of zinc on the lipid components of carp erythrocytes (Cyprinus carpio L.) were studied on red blood cells exposed to ZnSO4 (0.01 - 1 mM) for 20 h. The temperatures of the incubation medium were 5 and 20řC for carp collected in winter and summer, respectively. It was observed that increase in the concentration of metal ions in the medium led to significant changes in the content of the main phospholipid classes. Zinc induced the reduction of phosphatidylcholine as well as the increase in the content of phosphatidylserine and phosphatidylinositol. A marked increase in the levels of malondialdehyde and conjugated dienes was also found both in the cells and the incubation medium. Moreover, elevated Zn concentrations caused alterations in the erythrocyte plasma membrane fluidity, estimated by measuring the fluorescence of 1-[4-trimethylaminophenyl]-6-phenyl-1,3,5-hexatriene and 12-[9-anthroyloxy]stearic acid, located on the surface and in the hydrophobic regions of the lipid bilayer. This study in vitro confirmed that higher concentrations of zinc ions induce marked changes in the composition and structure of membrane lipids in carp erythrocytes.

365/2523

Tytuł oryginału: Tellurium-induced cognitive deficits in rats are related to neuropathological changes in the central nervous system.
Autorzy: Widy-Tyszkiewicz Ewa, Piechal Agnieszka, Gajkowska Barbara, Śmiałek Mieczysław
Źródło: Toxicol. Lett. 2002: 131 (3) s.203-214, il., tab., bibliogr. s. 213-214
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The effects of sodium tellurite 0.1 and 0.4 mg/kg were assessed in the Morris water maze. Two days after treatment rats were tested for acquisition (posttreatment days 3-6) and on seventh day on a spatial retention task. Tellurium treatment was found to cause significant impairment in retention of the spatial learning task. Locomotor disturbances were not the cause of the observed effects. Ultrastructural observations showed neuropathological changes in hippocampus subfields and prefrontal cortex with swelling of synapses, astrocytes and astrocytic processes around the vessels in the cerebral cortex neuropil. Severity of the observed changes in glial-neuronal unit was in correlation with the extent of learning impairment. A direct injury of Schwann cells with the secondary myoclasis was noted in the sciatic nerve. Our results indicate that acute treatment with sodium tellurite results in impairment of learning and spatial memory.

366/2523

Tytuł oryginału: Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production.
Autorzy: Tada Hiroyuki, Sugawara Shunji, Nemoto Eiji, Takahashi Nobuhiro, Imamura Takahisa, Potempa Jan, Travis James, Shimauchi Hidetoshi, Takada Haruhiko
Źródło: Infect. Immun. 2002: 70 (6) s.3304-3307, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 305,564

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.

367/2523

Tytuł oryginału: Fold-recognition analysis predicts that the Tag protein family shares a common domain with the helix-hairpin-helix DNA glycosylases.
Autorzy: Bujnicki Janusz M., Rychlewski Leszek
Źródło: DNA Repair 2002: 1 (5) s.391-395, il., bibliogr. 30 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The Escherichia coli protein Tag is traditionally regarded as an archetype of one of four classes of N-alkylpurine DNA glycosylases. However, its structure and phylogenetic relationship to other glycosylases emains a mystery. Fold-recognition and sequence profile analyses suggest that Tag shares the catalytic domain with helix-hairpin-helix (HhH) glycosylases such as MutY, AlkA and EndoIII, but its N- and C-termini together form a unique His2Cys2 cluster. The findings presented in this paper provide insight into sequence-structure-function relationships in the Tag family and should aid in a more precise definition of the common core of the HhH superfamily of glycosylases involved in DNA repair.

368/2523

Tytuł oryginału: International Journal of Cardiology
Tytuł angielski: Verapamil improves the pacing-induced vasodilatation in symptomatic patients with hypertrophic cardiomyopathy.
Autorzy: Dimitrow Paweł Petkow, Krzanowski Marek, Grodecki Janusz, Małecka Barbara, Lelakowski Jacek, Kawecka-Jaszcz Kalina, Szczeklik Andrzej, Dubiel Jacek S.
Źródło: Int. J. Cardiol. 2002: 83 (3) s.239-247, tab., bibliogr. 52 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The purpose of the study was to assess the effect of varapamil on the response of diastolic coronary flow velocity and coronary vascular resistance to pacing in symptomatic patients with hypertrophic cardiomyopathy. In 14 patients with hypertrophic cardiomyopathy, the coronary flow velocity was detected in the left anterior descending coronary artery using transthoracic Doppler echocardiography. The peak diastolic coronary flow velocity and coronary and coronary vascular resistance was measured at baseline and during pacing. Changes of these parameters induced by the pacing (expressed as the percentage of baseline values) were compared on varapamil treatment and after verapamil withdrawal. The same measurements were obtained in ten control subjects. The results show that, in hypertrophic cardiomyopathy patients, increase in coronary flow velocity during pacing was significantly higher on than off varapamil therapy (64.8 ń 32.5 vs. 41.1 ń 21.3 p.c., P 0.05). In control subjects, pacing-induced increase in coronary flow velocity was comparable to changes in coronary flow velocity in hypertrophic cardiomyopathy patients receiving verapamil (80.2 ń 18.4 vs. 64.8 ń 32.5 p.c., P 0.05). After verapamil withdrawal in hypertrophic cardiomyopathy patients, coronary flow velocity increase during pacing was significantly lower than in control subjects (41.1 ń 21.3 vs. 80.2 ń 18.4 p.c., P 0.05). During pacing the coronary vascular resistance decreased more on verapamil than after drug withdrawal (-34.7 ń 11.7 vs. -24.6 ń 12.9 p.c., P 0.05). In control subjects the coronary...

369/2523

Tytuł oryginału: Niguldipine impairs the protective activity of carbamazepine and phenobarbital in amygdala-kindled seizures in rats.
Autorzy: Borowicz Kinga K., Kleinrok Zdzisław, Czuczwar Stanisław J.
Źródło: Eur. Neuropsychopharmacol. 2002: 12 (3) s.225-233, il., tab., bibliogr. s. 232-233
Sygnatura GBL: 313,361

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: There is evidence that some calcium (Ca2+) channel inhibitors enhance the protective activity of antiepileptic drugs. Since clinical trials have not provided consistent data on this issue, the objective of this study was to evaluate the interaction of a dihydropyridine, niguldipine, with conventional antiepileptics in amygdala-kindled rats. Niguldipine (at 7.5 but not at 5 mg/kg) displayed a significant anticonvulsant effect, as regards seizure and afterdisharge durations in amygdala-kindled convulsions in rats, a model of complex partial seizures. No protective effect was observed when niguldipine (5 mg/kg) was combined with antiepileptics at subeffective doses, i.e. valproate (75 mg/kg), diphenylhydantoin (40 mg/kg), or clonazepam (0.003 mg/kg). Unexpectedly, the combined treatment of niguldipine (5 mg/kg) with carbamazepine (20 mg/kg) or phenobarbital (20 mg/kg) resulted in a proconvulsive action. BAY k-8644 (an L-type Ca2+ channel activator) did not modify the protective activity of niguldipine (7.5 mg/kg) or the opposite action of this dihydropyridine (5 mg/kg) in combinations with carbamazepine or phenobarbital. A pharmacokinetic interaction is not probable since niguldipine did not affect the free plasma levels of the antiepileptics. These data indicate that the opposite actions of niguldipine alone or combined with carbamazepine (or phenobarbital) were not associated with Ca2+ channel blockade. The present results may argue against the use of niguldipine as an adjuvant antiepileptic or for cardiovascular reasons in patients with complex partial seizures.

370/2523

Tytuł oryginału: Toxicology Letters
Tytuł angielski: Peripheral blood lymphocytes and selected serum interleukins in workers operating X-ray equipment.
Autorzy: Hrycek Antoni, Czernecka-Micińska Aleksandra, Kłuciński Piotr, Badowski Roman
Źródło: Toxicol. Lett. 2002: 132 (2) s.101-107, tab., bibliogr. s. 106-107
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: The objective of these studies was to determine the effect of low X-ray doses on peripheral blood lymphocytes in the workers operating X-ray equipment. In 30 workers and 18 persons who constituted the control group the absolute number of peripheral blood lymphocytes, the number of T CD4+ and T CD8+ lymphocytes, the ratio CD4+/CD8+ and the concentration of serum interleukins (IL-2, IL-4, IL-6) were tested. Subgroups with respect to sex and employment period were selected. A significant increase in IL-2 concentration in workers as compared to controls was observed and significantly decreased IL-4 level in operating X-ray equipment was revealed. The lowest levels of IL-4 were noted in the women subgroup. A high positive, statistically significant correlations between the number of lymphocytes and the concentration of IL-2 and the level of IL-4 were observed. Moreover, our studies revealed that lymphocyte number and interleukin concentrations in serum of X-ray operators do not depend on the assumed ranges of the length of employment period.

371/2523

Tytuł oryginału: Left ventricular true aneurysm without coronary artery occlusion - a case study.
Tytuł polski: Tętniak prawdziwy lewej komory serca bez zmian w naczyniach wieńcowych - opis przypadku.
Autorzy: Sadowski Jerzy, Wierzbicki Karol, Wójcik Sławomir, Wróbel Krzysztof
Źródło: Prz. Lek. 2002: 59 (3) s.190-192, il., bibliogr. 14 poz., streszcz.
Sygnatura GBL: 310,563

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: Wstęp: Tętniak prawdziwy lewej komory serca jest zdefiniowany jako wyodrębniona struktura w obrębie lewej komory serca, w której mięsień serca został zastąpiony tkanką włóknistą, z typową dyskinezą w czasie skurczu. Pełnościenny zawał serca z zamknięciem tętnicy zstępującej przedniej jest najczęstszą przyczyną powstania tętniaka prawdziwego lewej komory serca. Opis przypadku: 51 letni mężczyzna po zawale ściany dolno-bocznej przed 6 laty, został przyjęty w październiku 2001 roku do oddziału intensywnej terapii w Szpitalu Rejonowym z powodu epizodu zatrzymania krążenia w mechanizmie migotania komór w przebiegu powtórnego zawału ściany dolno-bocznej. Po opuszczeniu szpitala pacjent nie powrócił do pracy, odczuwał zmęczenie i duszność przy wysiłku. Wykonana rok później koronarografia (wcześniej chory nie wyrażał zgody na diagnostykę inwazyjną) ujawniła preawidłowy obraz naczyń wieńcowych z recesywną prawą tętnicą wieńcową. Lewa tętnica wieńcowa była szeroka z prawidłowym przepływem kontrastu. W wentrykulografii stwierdzono duży dyskinetyczny tętniak prawdziwy lewej komory serca w okolicy koniuszka wypełniony przyścienną skrzepliną. Frakcja wyrzutowa lewej komory wynosiła 30 proc. Chory został zakwalifikowany do aneurysmectomii. Śródoperacyjnie stwierdzono duży tętniak (8 cm) okolicy oniuszka, z którego usunięto świeżą skrzeplinę o wadze 9 g. Wykonano aneurysectomię metodą Stoneye'a. W badaniu histopatologicznym wycinka ściany tętniaka stwierdzono typowe włóknienie bez ...

Streszczenie angielskie: Background: Left ventricular true aneurysm is described as distinct area of the left ventricular wall with systolic dyskinesia where typical myocardial structure is replaced with fibrous tissue. Transmural infarction following occlusion of left anterior descending coronary artery is the most common cause of formation of the left ventricular aneurysm. Case report: A 51-year old white male, with the history of inferolateral wall myocardial infaction 6 years ago was admitted to the emergency department at the local hospital last year because of sudden cardiac arrest due to ventricullar fibrillation in the course of inferolated myocardial infarction. Later on the patient did not come back to work, felt very weak and had dyspnea on mild exertion. Coronary angiogram performed one year later (the patient refused coronary angiography examination at the time of myocardial infarction) showed normal coronary arteries with a recessive right coronary artery. The left coronary artery was wide with normal contrast flow. Ventriculography showed large, true dyskinetic aneurysm with mural thrombus in the apical segment of the left ventricular wall. Left ventricular ejection fraction was 30 p.c. Patient was qualified for the aneurysmectomy. A large dyskinetic aneurysm (8 cm) of the apical and anterolateral segment of the left ventricular wall was detected intraoperatively. A fresh thrombus weighing 9 g was evacuated from the inside of the aneurysm. Stoney's aneurysmectomy was ...

372/2523

Tytuł oryginału: Melanin potentiates gentamicin-induced inhibition of collagen biosynthesis in human skin fibroblasts.
Autorzy: Wrześniok Dorota, Buszman Ewa, Karna Ewa, Nawrat Piotr, Pałka Jerzy
Źródło: Eur. J. Pharmacol. 2002: 446 (1/3) s.7-13, il., bibliogr. s. 12-13
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: One of the recognized side effects of gentamicin is ototoxicity. The mechanism underlying the organ specificity of this side effect of gentamicin has not been fully established. In view of the fact that a number of pharmacologic agents are known to from complexes with melanin and melanin is an abundant constituent of the inner ear tissues, we determined whether gentamicin interacts with melanin and how this process affects the biosynthesis of collagen in cultured human skin fibroblasts. Our results indicate that gentamicin forms comples with melanin. The amount of gentamicin bound to melanin increases with increasing of initial drug concentration. The Scatchard plot analysis of drug binding to melanin showed that at least two classes of independent binding sites are implicated in gentamicin-melanin complex formation: one class with an association constant K1 ~ 4 x 10**3 M**-1, and the second class with an association constant K2 ~ 3 x 10**2 M**-1. The number of total binding sites (n1 + n2) was calculated as about 1.36 ćmol gentamicin per 1 mg melanin. We have suggested that prolidase, an enzyme involved in collagen metabolism, may be one of the targets for gentamicin-induced inhibition of collagen biosynthesis. We found that gentamycin-induced inhibition of prolidase activity (IC50 ~ 100 ćM) and collagen biosynthesis (IC50 ~ 100 ćM). At this concentration of gentamicin, DNA biosynthesis in human skin fibroblasts was inhibited only by about 30 p.c. Melanin at 100 ćg/ml produced about 25 p.c. inhibition of DNA synthesis and about 30 p.c. inhibition of prolidase activity, ...

373/2523

Tytuł oryginału: Synthesis and effects on chloroquine susceptibility in Plasmodium falciparum of a series of new dihydroanthracene derivatives.
Autorzy: Alibert Sandrine, Santelli-Rouvier Christiane, Pradines Bruno, Houdoin Carole, Parzy Daniel, Karolak-Wojciechowska Janina, Barbe Jacques
Źródło: J. Med. Chem. 2002: 45 (15) s.3195-3209, il.,tab., bibliogr. 47 poz.
Sygnatura GBL: 312,133

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca doświadczalna

Streszczenie angielskie: To suggest a mechanism of action for drugs capable to reverse the chloroquine resistance, a new set of 9,10-dihydro-9,10-ethano and ethenoanthracene derivatives was synthesized and compounds were tested with the aim to assess their effect on chloroquine susceptibility in Plasmodium falciparum resistant strains. With respect to this, reversal of resistance and change in drug accumulation were compared. Structure - activity relationship and molecular modeling studies made it possible to define a pharmacophoric moiety for reversal agents and to propose a putative model of interaction with some selected amino acids.

374/2523

Tytuł oryginału: Ethanol-induced neurotoxicity is counterbalanced by increased cell proliferation in mouse dentate gyrus.
Autorzy: Pawlak Robert, Skrzypiec Anna, Sulkowski Stanisław, Buczko Włodzimierz
Źródło: Neurosci. Lett. 2002: 327 (2) s.83-86, il., bibliogr. 19 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

myszy

Streszczenie angielskie: Chronic ethanol abuse leads to degenerative changes in the hippocampus, which may result in subsequent cognitive impairment. Since the hippocampus retains the ability to produce neurons throught adulthood, in the present study we examined if ethanol-induced neuronal loss could be counterbalanced by cell proliferation in mouse dentate gyrus (DG). A total of 14 days of ethanol administration resulted in marked increase in cells positive for TdT-mediated dUTP nick-end labeling in all hippocampal regions studied, indicating that neurons die throughout the hippocampus by apoptotic mechanism. However, cresyl violet staining revealed approximately 20 p.c. neuronal loss following ethanol administration in CA1 and CA2 fields (P 0.01 and P 0.05, respectively), but not in DG. At the same time ethanol caused 2-fold increase in the number of proliferating cells in subgranular zone of DG. Thus, long-term ethanol intoxication causes permanent damage to CA1 and CA2, but not to DG which can be counterbalanced by ongoing neurogenesis.

375/2523

Tytuł oryginału: Immune reconstitution after haematopoietic cell transplantation in children: immunophenotype analysis with regard to factors affecting the speed of recovery.
Autorzy: Kalwak Krzysztof, Gorczyńska Ewa, Toporski Jacek, Turkiewicz Dominik, Słociak Małgorzata, Ussowicz Marek, Latos-Grażyńska Elżbieta, Król Marzena, Bogusławska-Jaworska Janina, Chybicka Alicja
Źródło: Br. J. Haematol. 2002: 118 (1) s.74-89, il., tab., bibliogr. s. 87-89
Sygnatura GBL: 303,964

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Immune reconstitution was studied prospectively in 66 children who underwent 77 haematopoietic cell transplantations (HCT): 46 autologous HCTs in 39 patients and 31 allogeneic HCTs in 27 patients. We studied the dynamic analysis of immune recovery with regard to potential factors affecting its speed, including age, type of HCT, diagnosis, graft-versus-host disease (GvHD) and cytomegalovirus (CMV) infection reactivation. Absolute counts of different lymphocyte subsets and immunoglobulin serum levels were determined in peripheral blood of patients on d -7 and +16, and then at various intervals up to 24 months post transplant. Common patterns of immune recovery after both allogeneic and autologous HCT were identified: (I) CD4+CD45RO+ peripheral T-cell expansion on d +16; (II) inverted CD4+:CD8+ ratio from D+30 onwards; (III) rapid natural killer (NK) cell (CD16 ń CD56+) count normalization. We observed prolonged T-cell lymphopenia (CD3+, CD3+CD4+, CD4+CD45RA+) until 24 months after autologous HCT, whereas in the allogeneic setting CD3+CD4+ cells, including naive CD45RA+ cells, returned to normal values at 9 months post transplant. Age 10 years and coexistence of GvHD and CMV reactivation were associated with a substantial delay in T- (CD4+, including CD45RA+) and B-cell recovery after allogeneic HCT. Multidrug GvHD prophylaxis resulted in impaired T-(CD4+, CD4+CD45RA+) and B-cell reconstitution only in the early phase after allogeneic HCT (up to 4 months). Our results demonstrated that T-cell recovery was severely impaired in children after autologous HCT...

376/2523

Tytuł oryginału: Correlation of blood lymphocyte CTLA-4 (CD152) induction in Hodgkin's disease with proliferative activity, interleukin 2 and interferon-gamma production.
Autorzy: Kosmaczewksa Agata, Frydecka Irena, Boćko Dorota, Ciszak Lidia, Teodorowska Renata
Źródło: Br. J. Haematol. 2002: 118 (1) s.202-209, il., tab., bibliogr. s. 207-209
Sygnatura GBL: 303,964

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Expression of the downregulatory CTLA-4 molecule was determined on unstimulated and anti-CD3+ recombinant interleukin 2 (rIL-2)-stimulated peripheral blood T cells in Hodgkin's disease (HD) and correlated with the T-cells' proliferative activity, IL-2 and interferon (IFN)-gamma production. There was a negligible percentage of CTLA-4+/CD3+ cells before culture. The mean percentage of CTLA-4+/CD3+ lymphocytes increased gradually, peaked after 72 h of stimulation and returned to basal values after 96 h of stimulation. The mean proportion of CTLA-4+/CD3+ cells from untreated patietns was significantly higer after 24, 48 and 72 h of stimulation comppared with controls. The mean percentage of CTLA-4+/CD3+ cells from patietns in clinical remission (CR) was lower than that of untreated patients, but remained significantly higher compared with controls. Lymphocytes from untreated HD patients showed impaired proliferative activity, IL-2 and IFN-gamma production compared with controls. The proliferative activity of the lymphocytes, IL-2 and IFN-gamma production remained significantly lower in CR compared with controls. The proportion of CTLA-4+/CD3+ cells negatively correlated with proliferative activity, IL-2 and IFN-gamma production in HD patients and controls. However, some untreated patients as well as patients in CR with normal mean fluorescence intensity values of CTLA-4 showed unimpaired T-cell function tests. Our study provides the first evidence of an increased expression of downregulatory CTLA-4 molecule on stimulated T-cells in HD, which could be one of the mechanisms of immune deficiency in this disease.

377/2523

Tytuł oryginału: Photoreactivity of aged human RPE melanosomes: a comparison with lipofuscin.
Autorzy: Różanowska Małgorzata, Korytowski Witold, Różanowski Bartosz, Skumatz Christine, Boulton Mike E., Burke Janice M., Sarna Tadeusz
Źródło: Invest. Ophthalmol. Vis. Sci. 2002: 43 (7) s.2088-2096, il., bibliogr. 57 poz.
Sygnatura GBL: 311,976

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Purpose. To determine whether aging is accompanied by changes in aerobic photoreactivity of retinal pigment epithelial (RPE) melanosomes isolated from human donors of different ages, and to compare the photoreactivity of aged melanosomes with that of RPE lipofuscin. Methods. Human RPE pigment granules were isolated from RPE cells pooled into groups according to the age of the donors. Photoreactivity was determined by blue-light-induced oxygen uptake and photogeneration of reactive oxygen species. Short-lived radical intermediates were detected by spintrapping, hydrogen peroxide by an oxidase electrode, singlet oxygen by cholesterol assay, and lipid hydroperoxides by iodometric assay. Results. Blue-light photoexcitation of melanosomes resulted in age-related increases in both oxygen uptake and the accumulation of superoxide anion spin adducts. The efficiencies of these processes, however, were still significantly lower than that induced by photoexcited lipofuscin. During irradiation of melanosomes, a substantial amount of oxygen was converted into hydrogen peroxide, whereas for lipofuscin, hydrogen peroxide accounted for not more than 3 p.c. of oxygen consumed. In contrast to lipofuscin, photoexcited melanosomes did not substantially increase the rate of oxidative reactions in the presence of polyunsaturated lipids of albumin. However, oxygen uptake was significantly elevated in the presence of ascorbate. Thus, the rate of photo-induced oxygen uptake in samples containing both ascorbate and melanosomes approached that observed in lipofuscin samples...

378/2523

Tytuł oryginału: Human papillomavirus and oncogenesis: critical evaluation of recent findings.
Autorzy: Majewski Sławomir, Jabłonska Stefania
Źródło: Int. J. Dermatol. 2002: 41 (6) s.319-320, bibliogr. 16 poz.
Sygnatura GBL: 305,645

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

379/2523

Tytuł oryginału: Association of asthma and total IgE levels with human leucocyte antigen-DR in patients with grass allergy.
Autorzy: Woszczek G[rzeogrz], Kowalski M[arek] L., Borowiec M[aciej]
Źródło: Eur. Respir. J. 2002: 20 (1) s.79-85, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 312,824

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Exposure to grass pollens during the pollen season, reveals in sensitive patients symptoms of allergic rhinitis, conjunctivitis and/or bronchial asthma. It is not well understood why, in some patients, only symptoms of rhinits occur while in others similar exposure causes symptoms of asthma and rhinitis. An association study is reported here, where the possible contribution of human leucocyte antigen (HLA)-DR gene polymorphisms to differential phenotypic expression of symptoms in patients with grass-pollen allergy was determined. HLA-DR genotyping was performed by the polymerase chain reaction with the sequence-specific primers method in 82 patients with symptoms of seasonal allergic rhinitis and/or bronchial asthma and 52 healthy nonatopic control subjects. A significant association was found between HLA-DRB1*02, B5* haploytpe and asthma phenotype in patients with grass-pollen allergy when compared to patients with rhinitis only. Significantly higher total serum immunoglobulin E levels were observed in patients with HLA-DRB1*01 alleles in comparison to patients without these alleles. The data in this study suggest that human leucocyte antigen-DR locus, or other genes in linkage disequilibrium, may play an important role in asthma phenotype expression in patients with grass-pollen allergy as well as in determining total immunoglobulin E levels in these patients.

380/2523

Tytuł oryginału: Penicillin-binding proteins of Listeria monocytogenes - a re-evaluation.
Autorzy: Korsak Dorota, Zawadzka Joanna J., Siwińska Maria E., Markiewicz Zdzisław
Źródło: Acta Microbiol. Pol. 2002: 51 (1) s.5-12, il., tab., bibliogr. [23] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Intact Listeria monocytogenes cells or membranes isolated from them were treated with [3H]penicillin to allow identification of the penicillin binding proteins (PBPs) located in the cytoplasmic membrane. In the former case the PBPs were released from the cells following disruption of the cell wall murein with Listeria monocytogenes bacteriophage lysin. The procedure described by Dougherty et al. (1996) for Escherichia coli, with some modifications, was used to evaluate the Mrs of the individual PBPs and allowed direct quantitation of their copy number.

381/2523

Tytuł oryginału: Diagnostic methods for detection of respiratory RNA viruses.
Autorzy: Filipowicz Elżbieta
Źródło: Acta Microbiol. Pol. 2002: 51 (1) s.13-21, il., bibliogr. [32] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: This article is a comprehensive description of diagnostic methods for detection of RNA respiratory viruses - respiratory syncytial virus RSV, influenza A and B viruses, parainfluenza 1, 2 and 3 viruses, coronaviruses and rhinoviruses - from cell culture to molecular biology methods. Both patients and medical personnel appear to be at risk of viral infection, specially during the winter season. Moreover, many health care units lack viral diagnostic facilities: therefore, it is essential for medical personnel to have an understanding of the etiology, mechanisms of transmission and of all disposable today diagnostic methods of RNA respiratory viruses. Patients at greatest risk of acquring nosocomial viral respiratory disease are children, patients with immunodeficiency and patients treated in intensive care.

382/2523

Tytuł oryginału: Autoaggregation, hydrophobic, and hydrophylic properties of Moraxella catarrhalis strains.
Autorzy: Janicka Grażyna, Mikucka Agnieszka, Sękowska Alicja, Zwierzchlewski Tomasz, Wróblewski Marcin
Źródło: Acta Microbiol. Pol. 2002: 51 (1) s.23-30, il., bibliogr. [27] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The hydrophobicity of the bacterial cell surface was evaluated via the salt aggregation test (SAT) in 58 strains (19 from the lower and 39 from the upper respiratory tracts) of Moraxella catarrhalis in hospitalized patients aged 25 to 65. Based on the SAT results, the strains were divided into three groups: autoaggregating (highly hydrophobic), hydrophobic, and hydrophilic. At a temperature of 37řC, the autoaggregating, hydrophobic or hydrophilic properties did not depend on the choice of a medium, whereas at 22řC the investigated properties did (p 0.0001). Taking into account the origin of the strains (lower vs. upper respiratory tract), it was found that: in the strains cultivated in liquid medium, both highly hydrophobic, hydrophobic and hydrophilic surfaces were present with a comparative frequency, independent of the strain isolated site and cultivation conditions; strains with highly hydrophobic and hydrophobic surfaces, but only those cultivated on solid media at 22řC, were much more often isolated from sputum rather than from nose and throat swabs, whereas a ststistically significant incidence of hydrophilic strains was found in samples from the uppeer rather than lower respiratory tract.

383/2523

Tytuł oryginału: A comparison of four susceptibility tests on Yersinia enterocolitica strains isolated from human and animal material.
Autorzy: Woźniak-Kosek Agnieszka, Kawiak Jerzy
Źródło: Acta Microbiol. Pol. 2002: 51 (1) s.31-37, il., tab., bibliogr. [14] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The results of antibiotic sensitivity tests are presented for 20 strains of Yersinia enterocolitica from human and animal material as well as for one commercial standard strain. Results obtained with four different methods of assays were compared. One of the methods used was flow cytometric Minimum Inhibitory Concentration (MIC) assay (FC-MIC). Results of all four tests were strains in the clinical surrounding are discussed.

384/2523

Tytuł oryginału: Evaluation of antiviral activity of different origin compounds by flow cytometry.
Autorzy: Siennicka Joanna, Trzcińska Agnieszka, Kańtoch Mirosław
Źródło: Acta Microbiol. Pol. 2002: 51 (1) s.79-83, il., bibliogr. [18] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Against many viral diseases caused for example by HSV, EBV, CMV, HIV, RSV, HCV for which vaccines are not available, chemiotherapeutics seem to have the principal significance. High progress in development of new antiviral compounds is observed. In addition to synthetic compounds a large number of naturally occuring substances have been shown to posses antiviral activity. One of such substances is tannic acid. In this study comparison of antiviral activity of tannic acid, acyclovir (ACV) and ganciclovir (GCV) against cytomegalovirus (CMV) is presented. The MRC5 cells infected with CMV and treated with different compounds were analyzed by flow cytometry and cythopatic effect inhibition test for inhibition of virus replication and by MTT assay for cytotoxity. It has been shown that tannic acid has antiviral activity against cytomegalovirus and that expression of virus antigens measured as median fluoroscence intensity (MFI) by flow cytometry can be used for evaluation of virus replication.

385/2523

Tytuł oryginału: Hepatic steatosis in patietns with hyperlipoproteinaemia - correlations between histological finding and lipid profile determined by ultracentrifugation.
Tytuł polski: Stłuszczenie wątroby u osób z HLP - korelacja obrazu histopatologicznego i profilu lipidowego określanego za pomocą ultrawirowania.
Autorzy: Tacikowski Tadeusz, Dzieniszewski Jan, Ciok Janusz, Nowicka Grażyna
Źródło: Adv. Clin. Exp. Med. 2002: 11 (2) s.155-161, tab., bibliogr. 41 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wprowadzenie. W ostatnim okresie coraz częściej analizuje się problem stłuszczenia wątroby, wywołanego czynnikami innymi niż alkohol lub zakażenie wirusowe. Najczęściej dotyczy to stłuszczenia wątroby na skutek otyłości, cukrzycy i hiperlipoproteinemii (HLP). Choroba stłuszczeniowa wątroby (fatty liver disease) ma swój naturalny przebieg i można ją podzielić na cztery stadia: I - proste stłuszczenie, II - stłuszczenie z zapaleniem, III - stłuszczenie z wrotnym włóknieniem, IV - stłuszczeniowa marskość wątroby. Cel pracy. Ustalenie zależności między zaawansowaniem stłuszczenia wątroby w badaniu histopatologicznym punktatu wątroby a stężeniami cholesterolu i trójglicerydów w surowicy w poszczególnych frakcjach lipoprotein ocenianych za pomocą ultrawirowania. Materiał i metoda. Zbadano prospektywnie 30 chorych z różnymi typami HLP, u których w badaniu histopatologicznym stwierdzono cechy stłuszczenia wątroby. Stopień zaawansowania choroby stłuszczeniowej wątroby określano według podanej wcześniej klasyfikacji histopatologicznej. Z analizy wykluczono przypadki chorych, u których stwierdzano markery wirusowego uszkodzenia wątroby HBV i HCV oraz chorych pijących alkohol w ilości większej niż 200 g/miesiąc ( w przeliczeniu na czysty alkhol). Wyniki. W analizowanej grupie chorych 11 pacjentów miało proste stłuszczenie wątroby (36,7 proc.), 10 (33,3 proc.) stłuszczenie z zapaleniem, a 9 (30 proc.) stłuszczenie z wrotnym włóknieniem. Średnie stężenie TG stwierdzono w I typie ...

Streszczenie angielskie: Background. In recent years the problem is ever more frequently analysed of hepatic steatosis caused by factors other than alcoholism or viral infection, mainly in obesity, diabetes mellitus and hyperlipoprotienaemia (HLP). Fatty liver disease has its natural history which can be divided into four stages: I - steatosis, simple fatty liver, II - steatosis with hepatitis or fatty liver hepatitis, or fatty liver hepatitis, III - fatty liver with portal fibrosis, IV - fatty cirrhosis. Objectives. Finding of correlations between advanced hepatic steatosis found in histological examination of liver biopsy specimen and serum concentrations of cholesterol and triglycerides in various lipoprotein fractions obtained by ultracentrifugation. Material and method. Thirty patietns with various HLP types were studied prospectively after finding in them hepatic steatosis in histological examination. The degree of hepatic steatosis was determined according to histopathological classification presented earlier. Cases with presence of virus hepatophaty markers HBV and HCV, and patietns with a history of alcohol drinking in amounts above 200 g of pure alcohol monthly were excluded from the study. Results. In the studied group 11 patietns had simple fatty liver (36.7 p.c.), ten had fatty liver hepatitis (33.3 p.c.) and mine had fatty liver with portal fibrosis (30 p.c.). The mean TG concentration found in type I of histological lesions (simple fatty liver) was 221.0 mg/100 mL, and it was ...

386/2523

Tytuł oryginału: Lipid peroxidation of cellular membranes following photodynamic therapy with photosensitizers: sulfoxaporphyrin, dithiaporphyrin and hematoporphyrin derivative.
Tytuł polski: Peroksydacja lipidów błon komórkowych w następstwie terapii fotodynamicznej z użyciem fotouczulaczy: sulfoksaporfiryny, ditiaporfiryny oraz pochodnej hematoporfiryny.
Autorzy: Ziółkowski Piotr, Symonowicz Krzysztof, Bronowicz Andrzej, Osiecka Beata J., Chmielewski Piotr, Latos-Grażyński Lechosław, Dzięgiel Piotr
Źródło: Adv. Clin. Exp. Med. 2002: 11 (2) s.181-186, il., tab., bibliogr. 22 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie polskie: Wprowadzenie. Fotodynamiczna terapia (PDT) jest znaną metodą leczenia nowotworów złośliwych obejmującą stosowanie fotouczulaczy oraz światła o odpowiedniej długości fali. Mechanizmy działania PDT nie są całkowicie wyjaśnione, szczególnie gdy stosuje się nowe związki. Cel pracy. Celem pracy było sprawdzenie, czy nowe fotouczulacze w warunkach PDT mogą uszkadzać błony komórkowe i tym samym przyczyniać się do śmierci komórkowej. Materiał i metody. Sulfoksaporfiryna (OXA) i ditiaporfiryna (DTP) były podawane w iniekcjach dootrzewnowych myszom BALB/c uprzednio zaszczepionym mięsakiem włóknistym, BFS1. Stosowano dawki 2,5, 5,0 oraz 7,5 mg/kg w 0,9 p.c. Na Cl, a po upływie 24 godzin nowotwory naświetlano światłem o całkowitej dawce równej 50 lub 100J/cmý i długości fali wynoszącej 670 nm (OXA), 695 nm (DTP) oraz 627 nm (HpD). Po 30 minutach oraz po 6 i 48 godzinach myszy uśmiercano, pobierano nowotowory i poddawano je oznaczeniom w kierunku peroksydacji lipidów (LP). LP mierzono spektrofotometrycznie z użyciem zestawów dla malonylodialdehydu (MDA) i 4-hydroksyalkenów (4HNA). Wartości LP podawano w ćM. MDA+44HNA na gram tkanki. Wyniki. Stężenie LP dla poszczególnych fotouczulaczy nie różniło się znacząco i było zależne od stosowanej dawki fotodynamicznej. Najwyższe stężenia LP występowały po 6 godzinach od naświetlenia dawką 100 J/cmý (dawki fotodynamicznej. Najwyższe stężenia LP występowały po 6 godzinach od naświetlenia dawką 100 J/cmý (dawka preparatu - 7,5 mg/kg). W ...

Streszczenie angielskie: Background. Photodynamic therapy (PDT) is known method of anticancer treatment involving application of photosensitizer and subsequent irradiation with light of appropriate wavelength. Mechanisms of PDT action are not completely elucidated, especially when new photosensitizers are applied. Objectives. Aim of study was checking whether new compounds in terms of PDT impair cellular membranes, theus facilitating cell death. Material and methods. Sulfoxaporphyrin (OXA) and dithiaporphyrin (DTP) were injected intraperitoneally to BALB/c mice transplanted with fibrosarcoma BFS1 in doses 2.5, 5.0, 7.5 mg/kg in 0.9 p.c. NaCl. After 24 hours tumors were irradiated at total doses 50 or 100 J/sq cm and wavelength 670 nm (OXA), 695 nm (DTP) and 627 nm (HpD). Following 30 minutes, 6 and 48 hours observation mice were sacrified, tumors excised, and processed for lipid peroxidation (LP). LP was spectrophotometrically measured using kits for malonyldialdehyde (MDA) and 4-hydroxyalkenes (4HNA). LP was given in ćM MDA+4 HNA/g of tissues. Results. Level of LP for particular photosensitizer did not very significantly. LP showed to be photodynamic dose-dependent (photosensitizer x light doses). The highest levels of LP occurred after 6 hours from light application at 100 J/sq cm (compound dose was 7.5 mg/kg). In such conditions, OXA-PDT resulted in 998. 15, DTP-PDT in 899.04 and HpD-PDT in 1137.36 ćM/g, whereas in controls it did not exceed 73.0 ćM/g. At lates time point (48 hrs) these ...

387/2523

Tytuł oryginału: Artificial peritoneum for the treatment of fecal/barium peritonitis - an expreimental study.
Tytuł polski: Sztuczna otrzewna w leczeniu kałowo-barowego zapalenia otrzewnej - badania doświadczalne.
Autorzy: Kielan Wojciech, Kozianka Juergen, Grzebieniak Zygmunt
Źródło: Adv. Clin. Exp. Med. 2002: 11 (2) s.193-199, il., bibliogr. 9 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnie

Streszczenie polskie: Cel pracy. Celem pracy było zbadanie na modelu doświadczalnym zapalenia otrzewnej u świń, czy i w jaki sposób proces zapalny w jamie otrzewnej rozprzestrzenia się przez część przeponową otrzewnej ściennej i czy czynność resorpcyjną części przeponowej otrzewnej ściennej można zmniejszyć w sposób sztuczny, pokrywająć ją przyjętą do badań gąbką HYPOL - sztuczną otrzewną. Materiał i metody. Do doświadczenia użyto 19 świń, które podzielono na dwie grupy. 4 Świnie były grupą kontrolną (dwie z zapaleniem otrzewnej bez zakładania sztucznej otrzewnej oraz dwie z założoną sztuczną otrzewną, ale bez wywołanego zapalenia otrzewnej). 15 świń miało śródoperacyjnie wywołane kałowo-barowe zapalenie otrzewnej oraz założoną gąbkę HYPOL - sztuczną otrzewną pod kopułę przepony. Po śmierci świń sztuczna otrzewna oraz przepona były badane w mikroskopie elektronowym. Wyniki. Dwie świenie, które miały założoną sztuczną otrzewną bez wywołanego zapalenia otrzewnej, przeżyły operację bez powikłań. Zwierzęta, które miały wywołane zapalenie otrzewnej bez założenia sztucznej otrzewnej zmarły do 4 godzin po zabiegu. Świnie, ktróe miały wywołane zapalenie otrzewnej i założoną sztuczną otrzewną pod kopułą przepony przeżyły 6-16 godzin po operacji i czas ten był istotnie dłuższy niż u zwierząt bez założonej sztucznej otrzewnej. Gdy przepona nie była pokryta sztuczną otrzewną, dochodziło do całkowitego zniszczenia otrzewnej ściennej przepony z przechodzeniem kryształów siarczanu baru przez przeponę. W ...

Streszczenie angielskie: Objectives. The study was intended to evaluate the pattern of spreading of intraabdominal sepsis through the diaphragm and to find out whether implantation of a polyurethane HYPOL sponge as an artificial peritoneum can reduce the spread of sepsis. Material and methods. In the experiment 19 swine were used in two groups: 4 as controls (2 with peritonitis without artificial peritoneum, two with artificial peritoeum but without peritonitis) and 15 animals that had fecal/barium peritonitis induced, and HYPOL sponge implanted under the diaphragm. When they died, the sponge and peritoneum were examined in scanning electron microscopy. Results. The swine that had artificial peritoneum implanted without peritonitis recovered normally. The animals that had peritonitis without implanting artificial peritoneum died within 4 hours. With peritonitis and diaphragmal peritoneum protected with HYPOL sponge, the time of survival was significantly longer. Where the artificial peritoneum was not used, diaphragmal peritoneum underwent complete destruction and barium sulfate crystals migrated through the diaphragm. With peritoenum protected with the HYPOL sponge, the mesothelium was preserved and migration of barium reduced. Conclusions. The evaluated artificial peritoenum, HPOL sponge, provides protection to the diaphragmal peritoneum in fecal/barium peritonitis. It acts as a mechanical fllter interfering with resortption and spread of the infection in peritonitis. HYPOL sponge might ...

388/2523

Tytuł oryginału: Adrenal gland myelolipoma - report of two cases.
Tytuł polski: Mielolipoma nadnerczy - opis dwóch przypadków.
Autorzy: Bronowicz Andrzej, Woźniak Zdzisław Michał, Ziółkowski Piotr, Sawicz-Birkowska Krystyna, Tarnawa Robert
Źródło: Adv. Clin. Exp. Med. 2002: 11 (2) s.263-265, il., bibliogr. 13 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Przedstawiono dwa przypadki chorych z guzami typu mielolipoma zlokalizowanymi w nadnerczach. U jednego z pacjentów zmiana była jednostronna, u drugiego występowała w obu nadnerczach. Prawidłowe rozpoznanie tego rzadkiego guza zostało postawione po usunięciu chirurgicznym. Przedoperacyjna diagnostyka tego typu zmian jest trudna i prowadzi często do mylnych rozpoznań.

Streszczenie angielskie: We report on adrenal gland myelolipoma: one uni- and second, bilateral. This very uncommon tumor was correctly diagnosed after surgical treatment. Preoperative, it always presents problems, thus often leading to misdiagnosis.

389/2523

Tytuł oryginału: The hobnail cell metaplasia of endosalpinx.
Tytuł polski: Metaplazja hufnalowata w jajowodzie.
Autorzy: Rabczyński Jerzy, Woźniak Zdzisław Michał, Ziółkowski Piotr, Hałoń Agnieszka
Źródło: Adv. Clin. Exp. Med. 2002: 11 (2) s.273-275, il., bibliogr. 10 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Komórki przybierające kształt gwoździ służących do mocowania podków do końskich kopyt stanowią element osobliwego obrazu spotykanego głównie w obrębie wyściółki nabłonkowej kobiecych narządów rodnych. Komórki hufnalowate są typowe dla wywodzących się z międzynercza raków jasnokomórkowych szyjki macicy, trzonu macicy i jajnika. Autorzy opisali rzadkie przypadki metaplazji hufnalowatej w jajowodzie w przebiegu pierwotnego raka surowiczego oraz przewlekłego zapalenia tego narządu.

Streszczenie angielskie: The epithelial cells expressing nails with horse-shoe appearance are elements of various pathological disorders of female genital tracts. The hobnail type cells are very common for cervical, endometrial and ovarian clear cells carcinomas derived from mesonephros. Similar appearance is demonstrated in some nonneoplastic disorders of endometrium and endosalpinx. Rare case of hobnail cell metaplasia of endosalpinx in primary serous cell carcinoma and salpingitis has been described.

390/2523

Tytuł oryginału: Changes in BRCA1 gene in patients with familial breast cancer in the Warsaw region of Poland.
Tytuł polski: Zmiany w genie BRCA1 u chorych na rodzinnego raka sutka, leczonych w Centrum Onkologii w Warszawie.
Autorzy: Paszko Zygmunt, Skasko Elżbieta, Wiśniewska Alicja, Konopka Bożena, Kluska Anna, Jagielska Agnieszka, Wieczorek Ewa, Janiec-Jankowska Aneta, Niwińska Anna, Pieńkowski Tadeusz, Mazur Słwomir
Źródło: Nowotwory 2002: 52 (2) s.97-103, il., bibliogr. 17 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Spośród przeszło 500 kobiet chorych na raka sutka, leczonych w Centrum Onkologii w Warszawie, wyselekcjonowano 52 probantki, które były obarczone rodzinnym występowaniem kilku raków sutka i innych nowotworów. Materiałem badawczym było DNA, izolowane z leukocytów krwi obwodowej. Procedurą PCR-SSCP posługiwaliśmy się w celu poszukiwania mutacji we wszystkich kodujących białko eksonach genu BRCA1. Fragmenty podejrzane o obecność muatcji poddawano sekwencjonowaniu. U 36 probantek w genie BRCA1 wykryto 11 rodzajów zmian w eksonach i 5 rodzajów zmian w intronach. U 19 (36,5 proc.) probantek nie wykryto w genie BRCA1 żadnych zmian. Wśród zmian eksonowych trzy z nich powszechnie uznawane są za mutacje patogenne: 5382insC (3 rodziny; 6 przypadków), T300G (2 probantki), 3819del5 (1 probantka). Spośród zmian intronowych zmiana IVS6 + 28 (C - A) podejrzewana jest przez nas o patogenne właściwości, gdyż umiejscowiona jest w obszarze intronu, który bierze udział w składaniu eksonów. Występowała ona u trzech sióstr (dwie chore i jedna zdrowa). Pozostałych 8 zmian, wykrytych w eksonach 11, 13 i 16 (A1186G, C2201T T2430C, C2731T, A3232G, A3668G, T4427C, A4956G), to polimorfizmy. Jedna z nich - A1186G występowała jako zmiana heterozygotyczna częściej u chorych na raka sutka (18,8 proc.), niż u kobiet zdrowych (4 proc.). Wśród badanych chorych zaobserwowaliśmy w eksonach genu BRCA1 częste nagromadzanie się u tej samej chorej kilku (3-6) polimorfizmów. Dwie heterozygotyczne zmiany (T300G i A1186G), znalezione w DNA z krwi dwóch nie spokrewnionych chorych, utraciło swą heterozygotyczność w DNA izolowanym z guza.

Streszczenie angielskie: 52 patient-probands with a family history of breast cancer and other tumours were selected from among 500 breast cancer patietns trated at the Maria Skłodowska-Curie Memorial Cancer Centre and Institute of Oncology. We studied DNA isolated from peripheral blood. The PCR-SSCP procedure was used for seeking mutations in all protein coding exons of the BRCA1 gene. Fragments suspected of mutation presence were subjected to sequencing. In 36 probands of the BRCA1 gene 11 types of changes were found in exons and 5 types in introns. In 19 (36.5 p.c.) probands no changes were detected within the BRCA1 gene. Among exon changes 3 were ones generally regarded as pathogenic mutations: 5382insC (3 families; 6 cases), T300G (2 probands), 3819delGTAAA (1 proband). Among intron changes we suspect the IVS6 +28 (C - mA) change to be pathogenic, since it is present in intron area participating in exons splicing. It was found in three sisters (two had breast cancer, one was healthy). The remaining 8 changes were disclosed in exons 11, 13 and 16 (A1186G, C2201T, T2430C, C2731T, A3232G, A3668G, T4427C, A4956G) and regarded as polymorphisms. One of them, A1186G, was, as a heterozygotic change, more frequent in breast cancer cases (18.8 p.c.) than in healthy women (4 p.c.). We observed frequent presence of several types of polymorhisms (3-6) in BRCA1 gene exons. Two heterozygotic changes (T300G and A1186G) inthe blood DNA of two unrelated patients lost their heterozygosity in tumour DNA.

391/2523

Tytuł oryginału: Survival and pattern of failure after postoperative radiation therapy for medulloblastoma in adult patients.
Tytuł polski: Ocena wyników i przyczyn niepowodzeń pooperacyjnego napromieniania dorosłych chorych na rdzeniaka płodowego.
Autorzy: Żółciak Agnieszka, Kępka Lucyna, Fijuth Jacek, Leszczyk Czesława
Źródło: Nowotwory 2002: 52 (2) s.104-109, il., tab., bibliogr. 22 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Cel. Ocena wyników pooperacyjnej radioterapii 27 dorosłych chorych na rdzeniaka płodowego (modulloblastoma). Materiał i metoda. Poddano analizie 27 choorych na rdzeniaka płodowego, leczonych napromienianiem w II Zakładzie Teleradioterapii Centrum Onkologii im. M. Skłodowskiej-Curie, w latach 1984-2000. Dwudziestu sześciu chorych napromieniono na oś móżgowo-rdzeniową (mediana - 33,6 Gy), podwyższając dawkę na tylny dół czaszki do 55 Gy. Jeden chory był napromieniony paliatywnie na móżg. Chorym nie podawano chemioterapii jako pierwotnego leczenia. Przeprowadzono analizę niepowodzeń leczenia, przeżycia całkowitego, przeżycia bez nawrotu choroby i przeżycia po wznowie (PPW). Oceny czynników rokowniczych dokonano w analizie jednowymiarowej, włączając wiek, stan ogólny, stan neurologiczny, zakres resekcji, lokalizację guza (robak versus półkula móżdżku), obecność desmoplazji i obecność zastawki w komorze mózgu. Do analizy nie włączono stopnia zaawansowania ze względu na brak wykonywania wszystkich obowiązujących badań wyjściowych (rezonans rdzenia kręgowego i punkcja lędźwiowa). Wyniki. Przeżycie 5- i 10-letnie wyniosło odpowiednio 55 oraz 44 proc. Prawdopodobnieństwo 5- i 10-letniego przeżycia bez nawrotu choroby wynosiło odpowiednio 44 i 31 proc. Wiek okazał się jedynym istotnym czynnikiem prognostycznym w analizie jednowymiarowej. Pięcioletnie przeżycie całkowite dla chorych w wieku poniżej 30 lat wynosiło 40 proc., a dla chorych w wieku 30 i więcej lat 90 proc. (p = ...

Streszczenie angielskie: Aim. To present the results of postopeorative radiotherapy in 27 adult patients with medulloblastoma and to design future management strategies. Material and methods. 27 patients with medulloblastoma were treated between 1984 and 2000, of whom 26 received craniospinal irradiation (median 33.6 Gy) with a boost to the posterior fossa (55 Gy). No patient received chemotherapy at primary treatment. Pattern of failure, overall survival (OS), disease free survival (DFS) and survival after recurrence (SAR) were analysed. Age, tumour location, desmoplasia in histology examination, extent of resection, shunt presence, performance and neurologic status were included in a univariate analysis in order to assess possible factors influencing overall survival. The disease stage was disregarded because of insufficient initial staging. Results. Actuarial 5- and 10- year OS rates were 55 p.c. and 44 p.c., respectively. Actuarial 5- and 10- year DFS rates were 44 p.c. and 31 p.c. respectively. In univariate analysis age was the only significant prognostic factor for OS. For patients less than 30 years of age 5-year OS rate was 40 p.c., for elders - 90 p.c., p = 0.004. A tendency to improve OS was observed with better performance and neurologic status, and location in the cerebellar hemisphere, whilst the presence of shunt and gross total resection worsened OS without statistical significance. Tumour recurred in 17 (63 p.c.) patietns. Ten relapses (59 p.c.) occurred within the first 2 ...

392/2523

Tytuł oryginału: Radiotherapy outcomes in laryngeal cancer - a retrospective study.
Tytuł polski: Wyniki radioterapii chorych na raka krtani - analiza retrospektywna.
Autorzy: Hliniak Andrzej, Michalski Wojciech, Szutkowski Zbigniew, Jarząbski Andrzej, Laskus Zofia, Frenkiel Zofia, Osmólski Antoni, Rolski Wojciech
Źródło: Nowotwory 2002: 52 (2) s.111-117, il., tab., bibliogr. 12 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

otorynolaryngologia

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Celem pracy jest analiza wyników radykalnej radioterapii chorych na raka krtani w zależności od typowych czynników klinicznych takich jak: wiek, płeć, stopień zaawansowania choroby, stopień histopatologicznego zróżnicowania, stopień sprawności chorego, lokalizacja nowotworu, poziom hemoglobiny we krwi oraz zbadanie znaczenia potencjalnych czynników rokujących takich jak: intensywność palenia papierosów oraz występowanie choroby wieńcowej i stanów pogruźliczych w płucach. Analizowano retrospektywnie informację o 372 chorych leczonych w latach 1989-1995 samodzielnie i radykalnie promieniami w Zakładzie Teleradioterapii II Centrum Onkologii - Instytutu w Warszawie. Charakterystyka materiału: 88 proc. mężczyzn, 12 proc. kobiet, wiek: 29-82 lat, zaawansowanie: T1-20 proc., T2-34 proc., T3 - 30 proc., T4 - 16 proc., przerzuty do węzłów chłonnych: 27 proc. Całkowitą odpowiedź na leczenie (CR) uzyskano w 71 proc. przypadków. Wyleczenia miejscowe po 2 latach wynoszą 52 proc. (T1-T2 - 64 proc., T3-T4 - 40 porc.). Wczesne reakcje : ból w przełykaniu, zlewne zapalenie błony śluzowej i złuszczanie wilgotne odnotowano odpowiednio w 69 proc., 48 proc. i 41 proc. przypadków. Ciężkie powikłania późne stwierdzono u 19 pacjentów. Stwierdzono istotny wpływ stopnia zaawansowania klinicznego T i N oraz stopnia sprawności na ryzyko zgonu. Pacjenci w późnym stopniu zaawansowania (T3 - T4) mieli dwukrotnie wyższe ryzyko zgonu od pacjentów we wczesnym stopniu zaawansowania (T1 - T2). Ryzyko ...

Streszczenie angielskie: Study aim. To analyze the importance of pre-treatment factors (age, sex, T, N, histological differentiation, site, Hb level, performance status) and to investigate the influence of cigarette smoking and pulmonary and cardiac diseases on treatment oucomes in laryngeal cancer patients. Material and methods. From the year 1989 until May 1995 372 consecutive patietns with cancer of the larynx were radically irradiated at the 2nd Telradiotherapy Department of The Maria Skłodowska-Curie Memorial Cnacer Centre and Institute of Oncology in Warsaw (MSCMCC). Pt. characteristics - 88 p.c. men; 12 p.c. women, age: 29-82 years, stages: T1 - 20 p.c., T2 - 34 p.c., T3 - 30 p.c., T4 - 16 p.c., lymph node metastases: 27 p.c. Results. Complete response to treatment (CR) - 71 p.c. of cases. Loco-regional control after 2 years 52 p.c. (T1-T2 - 64 p.c., T3-T4 - 40 p.c.). Early reactions: pain on swallowing confluent mucositis and most skin reaction in 69 p.c., 48 p.c. and 41 p.c. of cases, respectively. Serious late complications - 19 patietns. In a majority of these cases several forms of serious damage were observed. Patietns with advanced disease (T3 - T4) present a two times higher death risk as compared to patienys in earlier stages of the disease (T1-T2). Patients with cervical node metastases also present a two times higher death risk as compared to NO cases. Patients with performance status 1 or more had a respectively three or four times hither death risk than patients with ...

393/2523

Tytuł oryginału: Clinical profile and outcome of patietns with cardiac myxomas treated surgically. A 20-yera experience with long-term follow-up.
Tytuł polski: Przebieg kliniczny i wyniki leczenia chirurgicznego u chorych ze śluzakami serca - 20 lat doświadczeń.
Autorzy: Jemielity Marek, Perek Bartłomiej, Katarzyński Sławomir, Misterski Marcin, Dyszkiewicz Wojciech
Źródło: Nowotwory 2002: 52 (2) s.118-122, il., tab., bibliogr. 18 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wstęp. Pierwotne nowotwory serca należą do rzadkości. Pośród nich łagodne śluzaki są najczęstszymi pierwotnymi guza serca. Celem pracy było przedstawienie charakterystyki klinicznej i ocena odległych wyników leczenia chorych operowanych z powodu śluzaków serca. Chorzy i metoda. Od 1981 r. do 2000 r. operaowano 21 chorych (12 kobiet i 9 mężczyzn), w wieku od 21 do 74 lat (średnio 43,9 ń 14,5 lat). Przed operacją chorych poddano badaniu fizykalnemu, wykonano badanie EKG, zdjęcie radiologiczne klatki piersiowej, echokardiografię (M + 2D) i koronografię (u chorych powyżej 40 lat). Wszystkich operowano ze sternotomii pośrodkowej w umiarkowanej hipotermii (26-28řC), z użyciem krążenia pozaustrojowego. Podczas ostatniej obserwacji odległej oceniano stan kliniczny i ponownie wykonano echokardiografię (M + 2D). Wyniki. Przed operacją u 19 chorych (90,5 proc.) stwierdzono objawy typowe dla zwężenia lewego ujścia żylnego. Sześciu spośród nich (28,6 proc.) znajdowało się W II klasie wydolności według Nowojorskiego Towarzystwa Kardiologicznego (NYKA), 8 chorych (38,1 proc.) w klasie III i 5 (23,8 proc.) w klasie IV. Epizody zatorowe przed operacją obserwowano u 6 chorych (28,6 proc.). Dziesięciu chorych (47,6 proc.) prezentowało niespecyficzne objawy ogólne, w tym przewlekłe gorączki i znaczną utratę masy ciała. Dwóch chorych (9,5 proc.) było bezobjawowych przed opracją. We wszystkich przypadkach ostateczne rozpoznanie stawiano na podstawie badania echokardiograficznego. Śluzaki ...

Streszczenie angielskie: Introduction. Primary neoplasms of the heart are very rare, benign cardiac myxomas being the most common. The aim of this study was to present the clincial profile and long-term outcome of patietns (pts) who underwent surgical resection of cardiac myxoma. Material and methods. Between 1981 and 2000, 21 pts (12 female and 9 male), mean age 43.9 ń 14.5 years (range: 21-74 yrs) underwent surgery for cardiac myxoma. Before surgery we performed physical examination, ECG, chest X-ray, echocardiography (M + 2D) and coronary artery angiography (in patietns over 40 years of age). All pts were operated by median sternotomy in moderate hypotheramia (26-28řC) with the use of cardiopulmonary bypass. At the end of the follow clincial examination and echocardiography (M + 2D) were performed. Results. Before operation 19 pts (90.5 p.c.) presented with cardiac signs related to mitral orifice obstruction. Six of them (28.6 p.c.) were NYHA class II, 8 (38.1 p.c.) - class III and 5 (23.8 p.c.) - calss IV. Events of embolism occurred in 6 pts (28.6 p.c.). Ten pts (47.6 p.c.) had nonspecific systemic or constitutional symptoms, including permanent fever and significant weight loss. Two pts (9.5 p.c.) were asymptomatic, diagnosis being established accdentally. In all cases definite diagnosis was made by echocardiography. The myxomas were located in the left atrium in 18 pts (85.7 p.c.), in the right atrium in 2 pts (9.6 p.c.) and in the right ventricle in one pt (4.7 p.c.). One pt (4.7 ...

394/2523

Tytuł oryginału: 3- mercaptopyruvate sulfurtransferase and rhodanese activities in human myometrium and leiomyomas of the uterus.
Tytuł polski: Aktywność transferazy siarkowej 3-merkaptopirogronianu i rodanazy w mięśniu i mięśniaku macicy ludzkiej.
Autorzy: Frendo Julian, Wróbel Maria, Wąs Kazimierz
Źródło: Nowotwory 2002: 52 (2) s.123-125, il., tab., bibliogr. 20 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Transferaza siarkowa 3-merkaptopirogronianu (EC 2.8.1.2) i rodanaza (transferaza siarkowa tiosiraczan: cyjanek, EC 2.8.1.1) uczestniczą w procesie desulfuracji L-cysteiny, prowadzącym do tworzenia związków zawierających metaboilcznie aktywną zredukowaną siarkę, tak zwaną siarkę suflanową, mającą wpływ na proliferację komórek nowotworowych. W przypadku niektórych tkanek nowotworowych obserwowano znacznie obniżoną aktywność transferazy siarkowej 3-merkaptopirogronianu i rodanazy,jak również śladową ilość siarki sulfanowej. Podjęto badania porównawcze aktywności obydwu enzymów w tkance mięśniaka ludzkiego oraz mięśnia macicy, z którego się rozwinął. Oznaczenia wykonywano natychmiast po otrzymaniu tkanek, uzyskanych w trakcie zabiegu chirurgicznego usuwania nowotworu. Niespodziewanie stwierdzono, że aktynwność obydwu badanych enzymów jest wyższa w mięśniaku, w porównaniu do tkanki niezmienionej nowotworowo, co można tłumaczyć tym, że jest to nowotwór łagodny.

Streszczenie angielskie: Background. 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2) and rhodanese (thiosulfate: cyanide sulfurtransferase, EC 2.8.1.1) participate in L-cysteine desulfuration - the main source of metabolically active sulfane sulfur atoms, which possibly influence the proliferation of malignant cells. It has been demonstrated that 3-mercaptopyruvate sulfurtransferase and rhodanese activity can decrease in some transplanted neoplsms. Aim. To examine tumour tissue of the human uterus and to investigate the myometrium from which the growth developed. Methods. The activity of 3-mercaptopyruvate sulfurtrnasferase and rhodanese was assayed in myometrium and leiomyoma samples immediately after hysterectomies. Results. The activities of two sulfurtransferases are higher in the leiomyoma than in the myometrium. Conclusion. An increase in the activity of both sulfurtransferases in leiomyomas of the uterus is surprising in light of present day understanding of sulfur compound metabolism in neoplasms, and may be due to the fact that leiomyoma is a benign neoplasm.

395/2523

Tytuł oryginału: The usefulness of magnetic resonance imaging (MRI) in cervical carcinoma assessment a preliminary report.
Tytuł polski: Wartość diagnostyczna rezonansu magnetycznego (MR) w ocenie raka szyjki macicy - doniesienia wstępne.
Autorzy: Tacikowska Małgorzata, Grzesiakowska Urszula, Tacikowski Tadeusz, Sobiczewski Piotr
Źródło: Nowotwory 2002: 52 (2) s.126-129, bibliogr. 11 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Cel. Zadaniem diagnostyki obrazowej nie jest wykrywanie raka szyjki macicy, lecz ocena stopnia zaawansowania. Celem pracy było: 1)porównanie badania MR z wynikami histopatologicznymi uzyskanymi po operacji, w ocenie wymiarów raka szyjki macicy; 2) analiza czułości i specyficzności badania MR w ocenie naciekania przymacicza; 3) ustalenie czułości i specyficzności badania MR w ocenie naciekania pochwy i macicy; 4) ocena przydatności badania w wykrywaniu przerzutów do węzłów chłonnych. Materiał i metoda. Materiał stanowiły badania MR miednicy, wykonane aparatem 2T firmy Elscint, u 15 chorych na raka szyjki macicy. Wiek chorych od 37 do 73 lat. Wszystkie chore były operowane w okresie nie dłuższym niż 30 dni od badania MR. Podczas badania MR wykonywano następujące sekwencje: przed podaniem środka kontrastowego (Gd DTPA) SE T1 w projekcji poprzecznej, po podaniu gadolimu SE T1 w projekcjach: poprzecznej, czołowej i strzałkowej, FSE T2 w projekcjach: poprzecznej, czołowej i strzałkowej. Wyniki. W ocenie wymiarów raka szyjki macicy badanie MR odznacza się wysoką zgodnością (p = 0.9389) z wynikami pooperacyjnego badania histopatologicznego. W ocenie naciekania przymacicza czułość i specyficznośc badania MR wynoszą odpowiednio: 75 proc. i 100 proc. W ocenie naciekania pochwy i trzonu macicy czułość i specyficzność badania MR wynoszą odpowiednio: 100 proc. i 85 proc. oraz 100 proc.i 100 proc. Badanie MR jest mało czułą (67 proc.), lecz wysoce specyficzną (100 proc.) metodą ...

Streszczenie angielskie: Aim. The aim of diagnostic is not so much the detection of cervical carcinoma, but the evaluation of its stage. In view of this the aim of this study included: 1) comparison of MR results with the results of histological examinations after operations with reference to the dimensions of cervical carcinoma; 2) assessment of the sensitivity and specificity of MRI in the evaluation of parametrium infiltration; 3) analysis of the sensitivity and specificity of MRI in the evaluation of infiltration of the vagina and uterus; 4) assessment of the usefulness of theis method in the detection of metastases to lymph nodes. Material and method. The material consisted of pelvic MRI, obtained with 2T Elscint unit in 15 patietns with cervical carcinoma, aged 37 to 73 years. All patietns underwent surgical treatment within 30 days after MR. During the MR examination the following sequences were performed: SE (spin echo) T1 (longitudinal relaxation time) in axial projection before administration of gadolinium (Gd-DTPA); SE T1 in axial, frontal and sagittal projections after contrast injection and FSE (fast spin echo) T2 (transversal relaxation time) in axial, frontal and sagittal projections. Results. 1) in the assessment of cervical carcinoma dimensions MRI results are highly concordant with the results of postoperative histological examination (p = 0.9389); 2) in the assessment of parametrium infiltration sensitivity and specificity of MRI are 75 p.c. and 10 p.c. respectively; 3) in ...

396/2523

Tytuł oryginału: Seventieth anniversary of the opening of the Radium Institute in Warsaw.
Autorzy: Towpik Edward, Sobieszczak-Marciniak Małgorzata, Mould Richard F.
Źródło: Nowotwory 2002: 52 (2) s.130-143, il.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

397/2523

Tytuł oryginału: Application of high-performance size exclusion chromatography to the determination of erythropoietin in pharmaceutical preparations.
Autorzy: Dudkiewicz-Wilczyńska Jadwiga, Snycerski Andrzej, Tautt Jadwiga
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.83-86, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: High performance size exclusion chromatography with fluorimetric detection for the determination of erythropoietin in pharmaceutical preparations has been developed. The applied chromatographic system has been enabled a quick estimation of the identity and contents of the studied compound. The method was validated and showed good validation data in terms of lineary, precision and repeatability. The validated method was successfully applied to the determination of erythropoietin content in commercially available preparations.

398/2523

Tytuł oryginału: Application of topological indexes for evaluation of the TLC separation of selected essential oil components.
Autorzy: Pyka Alina, Bober Katarzyna, Gurak Danuta, Niestroj Anna
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.87-91, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The selected essential oil components (menthol, (+)borneol, geraniol, linalool, carvone, camphor, (IR)-(-)fenchone) were separated by adsorption thin-layer chromatography using benzene as mobile phase. Investigated terpenes were characterized by selected topological indexes based on the adjacency and distance matrix. From among all the topological indexes counted only Si(o) index (the sum of the distance between the oxygen atom and the remaining atoms in graph) as well as cluster Randić indexes (4lambdac, and 4 lambdacv) allow for estimation of the chromatograms obtained. With the fact mentioned above, there exists the possibikity of prediction of relative situation on a chromatogram of the terpens investigated. For the rest of topological indexes that possibility was not stated.

399/2523

Tytuł oryginału: Determination of the content of vitamin K, in complex vitamin preparations by the HPLC method.
Autorzy: Kłaczkow Gabriela, Anuszewska Elżbieta L.
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.93-97, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In the present study we tried to use HPLC method to determine the vitamin K1 content in complex vitamin preparation. The reverse phases technique was applied and measurements were made at the wavelenght 248 nm. On the base of statistical analysis we can note that conditions for HPLC method used in our work gave us precise and accuirate results.

400/2523

Tytuł oryginału: Pharmaceutical availability of betamethasone dipropionate and gentamicin sulfate from cream and ointment.
Autorzy: Dyderski Stanisław, Grześkowiak Edmund, Szałek Edyta, Mrzygłód Agnieszka
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.99-103, il., tab., bibliogr. 6 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Kinetics of drug release from both compared preparations occuring as a cream and ointment, was in vitro studied. A reversed-phase HPLC method was developed for the determination of betamethasone dipropionate in lipophylic bases. Analyses were performed using a PLRP column with a mobile phase of methanol-acetonitrile-water and ultraviolet detection at lambda = 254 nm. The calibration curve was constructed for concentration 1.0 - 50.0 ćg/ml. The method is simple, accurate and precise. For the determination of gentamicin sulfate the FPIA method was used.

401/2523

Tytuł oryginału: Effect of microcrystalline chitosan on the solubility of ibuprofen.
Autorzy: Bodek Kazimiera H.
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.105-108, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: It was found that MCCh may be used for the enhancement of the solubility and dissolution rates of drugs. The solubility of IBA in water increased significantly (about 10-fold) when MCCh was added. Differential scanning calorimetry (DSC), powder X-ray diffractometry, and IR spectroscopy were used to confirm that salt links between the drug and MCCh occurred.

402/2523

Tytuł oryginału: Investigation of hypoglycemic properties of rectal suppositories with chloropropamide.
Autorzy: Kosior Anna
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.109-113, tab., bibliogr. 8 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

badanie porównawcze

Wskaźnik treści:

króliki

Streszczenie angielskie: Rectal suppositories with chlorpropamide and suppositories with chlorpropamide in the dispersion system with urea prepared. Witepsol H15 and H190 and a polyoxyethylenglycol mixture were used as bases. Rabbits were tested for blood-glucose level. The animals have been administered with prepared suppositories and commercial tablets. It was found the suppositories prepared on the basis Witepsol H15 with the dispersed chlorpropamide caused a much higher decrease of blood-glucose level than commercial tablets.

403/2523

Tytuł oryginału: Evaluation of adriamycin induced DNA damage and repair in human and animal cells.
Autorzy: Chłopkiewicz Bożena
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.115-120, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Adriamycin induced DNA damage and repair were investigated by the comet assay in four human cell lines (CRL2088, ME18, Lu106, WISH) and five animal cell lines (L929, Balb/3T3, CHO, MDBK, Vero). The results indicated the concentration-dependent induction of DNA strand breaks in all cell lines after adriamycin treatment. Simultaneously, wide differences in sensitivity of cell to the damaging action of adriamycin were observed. The most sensitive were two human cell lines derived from epithelial tissues, Lu106 and WISH. In these lines the breaks induced by adriamycin were not repaired within 6 h postincubation. In two other human cell lines, CRL2088 and ME18, only a small number of DNA breaks appeared after adriamycin exposure and these were very quickly repaired. Among the animal cell lines only the mouse cell line L929 was very sensitive to adriamycin, but most of the induced breaks were repaired within 6 h postincubation. The remaining animal cell lines were less sensitive to DNA damage by adriamycin and all except 3T3, completely repaired the breaks within 6 h postincubation.

404/2523

Tytuł oryginału: A new method for the synthesis of ranitidine.
Autorzy: Lipnicka Urszula, Jasztold-Howorko Ryszard, Witkiewicz Krystyna, Machoń Zdzisław
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.121-125, il.,bibliogr. 36 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: An improved synthesis of the antiulcer drug Ranitidine from an oxazolidine derivative is reported.

405/2523

Tytuł oryginału: Pyrrole analogues of chloramphenicol. [P.] 3: Synthesis and antibacterial activity of DL-threo-1-(1-methylsul-fonylpyrrole-3-YL)-2-dichloroacetamidopropane-1,3-diol.
Autorzy: Krajewska Dorota, Dąbrowska Marta, Jakoniuk Piotr, Różański Andrzej
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.127-132, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A seven-stage synthesis of a pyrrole analogue of chloramhenicol is described. The compound exhibits a significant antibacterial activity, over the 3 p.c. to 50 p.c. range of the chloramphenicol activity; over the 6 p.c. to 100 p.c. range of the thiamphenicol activity and florfenicol.

406/2523

Tytuł oryginału: Further flavonoids from the flowers of Prunus spinosa L.
Autorzy: Olszewska Monika, Wolbiś Maria
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.133-137, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Further seven flavonoidis were isolated from the flowers of Prunus spinosa L.: quercetin 3-0-ŕ-L-arabinopyranoside, 3-0-ŕ-L-rhamnopyranoside, 3-0-á-D-xylo-pyranoside, and 3-0-á-D-glucopyranoside, kaempferol 3,7-di-0-ŕ-L-rhamnopyranoside, kaempferol and quercetin 3-0-(4"-á-D-glucopyranosyl)-ŕ-L-rhamnopyranosides. Four of them have been found for the first time in this plant. Structural elucidation was performed by means of chemical and spectral methods (UV, LSI MS, 1D and 2D NMR).

407/2523

Tytuł oryginału: Flavonoids from lemon balm (Melissa officinalis L., Lamiaceae).
Autorzy: Patora Jolanta, Klimek Barbara
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.139-143, il., bibliogr. 20 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Six flavonoids have been isolated from the leaves of lemon balm (Melissa officinalis L., Lamiaceae). Their structures were determined on the basis of spectral data (UV, IR, 1H NMR, 13C NMR and FAB MS) as luteolin, luteolin 7-0-á-D-glucopyranoside, apigenin 7-0-á-D-glucopyranoside, luteolin 7-0-á-D-glucuronopyranoside, luteolin 3'-0-á-D-glucuronopyranoside and luteolin 7-0-á-D-glucopyranoside-3'-0-á-D-glucuronopyranoside. The last three glycosides have been found in lemon balm for the first time and nuteolin 7-0-á-D-glucopyranoside-3'-0-á-D-glucuronopyranoside is a new compound found in plants.

408/2523

Tytuł oryginału: Comparative study on the free flavonoid aglycones in herbs of different species of Polygonum L.
Autorzy: Smolarz Helena D.
Źródło: Acta Pol. Pharm. 2002: 59 (2) s.145-148, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The flavonoid aglycones were studied in the herbst of the following taxons of the Polygonum L. genus: P. hydropiper l., P. bistorta L., P. aviculare L., P. persicaria L., P. lapathifolium ssp. tomentosum (Schrank) Dans, P. lapathifolium ssp. nodosum (Pers.) Dans, P. amphibium L., P. mite Schrank, P. convolvus L. (Bilderdykia convolvuls L.) by means of the RP-HPLC method. The content of taxifolin, qurcetin, guercetin-3-methyl erher, kaemferol, myritcetin, luteolin, isorhamnetin and rhamnetin were determined.

409/2523

Tytuł oryginału: Functional and clinical characterization of KCNJ2 mutations associated with LQT7 (Andersen syndrome).
Autorzy: Tristani-Firouzi Martin, Jensen Judy L., Donaldson Matthew R., Sansone Valeria, Meola Giovanni, Hahn Angelika, Bendahhou Said, Kwieciński Hubert, Fidziańska Anna, Plaster Nikki, Fu Ying-Hui, Ptacek Louis J., Tawil Rabi
Źródło: J. Clin. Invest. 2002: 110 (3) s.381-388, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 310,487

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Andersen syndrome (AS) is a rare, inherited disorder characterized by periodic paralysis, long QT (LQT) with ventricular arrhythmias, and skeletal developmental abnormalitise. We recently established that AS is caused by mutations in KCNJ2, which encodes the inward rectifier K+ channel Kir2.1. In this report, we characterized the functional consequences of three novel and seven previously described KCNJ2 mutations using a two-microelectrode voltage-clamp technique and correlated the findings with the clinical phenotype. All mutations resulted in loss of function and dominant-negative suppression of Kir2.1 channel function. In mutation carriers, the frequency of periodick paralysis was 64 p.c. and dysmorphic features 78 p.c. LQT was the primary cardiac manifestation, present in 71 p.c. of KCNJ2 mutation carriers, with ventricular arrhythmias present in 64 p.c. While arrhythmias were common, none of our subjects suffered sudden cardiac death. To gain insight into the mechanismof arrhythmia susceptibility, we simulated the effect of reducdd Kir2.1 using a ventricular myocyte model. A reduction in Kir2.1 prolonged the termkinal phase of the cardiac action potential, and n the setting of reduced extracellular K+, nduced Na+/Ca2+ exchanger-dependent delayed afterdepolarizations and spontaneous arrhythmias. These findings suggest that the substrate for arrhythmia susceptibility in AS is distinct from the other forms of inherited LQT syndrome.

410/2523

Tytuł oryginału: 1- methyl-4-phenylpyridinium and 3-nitropropionic acid diminish cortical synthesis of kynurenic acid via interference with kynurenine aminotransferases in rats.
Autorzy: Luchowski Piotr, Luchowska Elżbieta, Turski Waldemar A., Urbańska Ewa M.
Źródło: Neurosci. Lett. 2002: 330 (1) s.49-52, il., bibliogr. 22 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The aim of the present study was to evaluate the effect of mitochondrial inhibitors, 1-methyl-4-phenylpyridinium (MPP+) and 3-nitropropionic acid (3-NPA), on the brain production of endogenous glutamate antagonist, kynurenic acid (KYNA). MPP+ and 3-NPA dose-dependently impaired the synthesis of KYNA in rat cortical slices. Enzymatic studies revealed the MPP+ inhibits in a concentration-dependent manner the activity of kynurenine aminotransferase II (KAT II), but not the activity of kynurenine aminotransferase I (KAT I). 3-NPA impaired the activity of both enzymes, KAT I and KAT II. Thus, MPP+ - and 3-NPA-evoked neurotoxicity may be at least partially associated with the depletion of KYNA.

411/2523

Tytuł oryginału: Cardiac arrest related to coronary spasm in patients with variant angina: a three-case study.
Autorzy: Seniuk W., Mularek-Kubzdela T[atiana], Grygier M[arek], Grajek S., Cieśliński A[ndrzej]
Źródło: J. Intern. Med. 2002: 252 (4) s.368-376, il., bibliogr. 33 poz.
Sygnatura GBL: 300,763

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We present three patients with variant angina pectoris and episodes of cardiac arrest. All of them had typical clinical symptoms, ST-segment changes in electrocardiogram, and coronary artery spasm confirmed by arteriography. They were treated with high doses of calcium antagonists and nitrates. An automatic cardioverter-defibrillator was implanted in the patient who developed ventricular fibrillation despite therapy with calcium antagonists. In another patient a DDD pacemaker was implanted becasue of high-degree atrioventricular blok.

412/2523

Tytuł oryginału: Porihvnjalnijj analihz sistem opodatkuvannja zarobihtnoji płati pracihvnikihv aptek v Ukrajinih hi Polshhih.
Tytuł angielski: The comparative analysis of systems of the taxation of wages of pharmacies workers i Ukraine and Poland.
Autorzy: Tereshhuk S. Ih., Moska Dionizy, Moska Dariusz, Tereshhuk T. O., Labus O.
Źródło: Farm. Zh. 2002 (3) s.27-32, il., tab., bibliogr. 9 poz., sum., rez.
Sygnatura GBL: 304,380

Hasła klasyfikacyjne GBL:

ekonomika

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The compareative analysis of taxacion systems of private individuals and ownershipers's wages in Ukraine and Poland has been carried out. It is established, that more flexible and transparent system of the taxes is used in Poland, according to which the base for the taxation decreases on living expenses of the citizens.

413/2523

Tytuł oryginału: Group II mGlu receptor agonists inhibit behavioural and electrophysiological effects of DOI in mice.
Autorzy: Kłodzińska Aleksandra, Bijak Maria, Tokarski Krzysztof, Pilc Andrzej
Źródło: Pharmacol. Biochem. Behav. 2002: 73 (2) s.327-332, il., bibliogr. s. 331-332
Sygnatura GBL: 312,498

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: It has been suggested that metabotropic glutamate (mGlu) receptor agonists selective for Group II mGlu receptors may have antipsychotic action. Therefore, we studied whether the effects, which could be related to psychotomimetic action of hallucinogenic drugs, are inhibited by Group II mGlu receptor agonists. The selective mGlu2/3 agonist LY354740 and LY379268 inhibited (ń)1-(2,5-dimethoxy-4-iodophenyl)-2-aminoproprane (DOI)-induced head twitches in mice in a dose-dependent manner. Furthermore, LY379268 suppressed an increase in the frequency of sponataneous excitatory synaptic potentials induced by bath-applied DOI in layer V pyramidal cells recorded in the murine medial frontal cortex. The data indicate that Group II mGlu receptor agonist may counteract the effects of hallucinogenic drugs.

414/2523

Tytuł oryginału: Sex hormone-binding globulin polymorphisms in familial and sporadic breast cancer.
Autorzy: Frsti Asta, Jin Quinren, Grzybowska Ewa, Sderberg Magnus, Zientek Helena, Siemińska Marzena, Rozgozińska-Szczepka Jadwiga, Chmielik Ewa, Utracka-Hutka Beata, Hemminki Kari
Źródło: Carcinogenesis 2002: 23 (8) s.1315-1320, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 312,779

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Ovarian steroids are one of the strongest risk factors for breast cancer. Sex hormone-binding globulin (SHBG) binds and transports sex steroids in the blood, regulating their bioavailable fraction and access to target cells. It can also inhibit the estradiol-induced proliferation of breast cancer cells through its membrane receptor. Three coding-region polymorphisms, which oead to an amino acid change, have been reported. We studied the influence of these three polymorphisms on breast cancer risk in three different populations: polish familial breast cancer cases, 27 p.c. of them carrying a BRCA1/BRCA2 mutation, Nordic familial and sporadic breast cancer cases. The reported G to A polymorphism in exon 1 was not found in the 423 analyzed samples. Instead, we found a C to T transition causing an arg to cys amino acid change within the same codon in one polish breast cancer patient and her daughter. Both of them were heterozygotes for the exon 8 to A polymorphism as well. They were diagnosed for bilateral breast cancer and carried a BRCA1 mutation (5382insC). Analysis of the tumor samples showed that they had lost the wild-type allele both at exons 1 and 8 of the SHBG gene. Analysis of the other Polish samples showed no correlation of the exon 8 polymorphism to breast cancer, bilateral breast cancer, BRCA1/BRCA2 mutations or age at diagnosis. No association of the exon 8 polymorphism with breast cancer in the Nordic familial or sporadic cases was found. The C to T polymorphism located in exon 4 was rare in all the studied populations (overal allele frequency 0.011)...

415/2523

Tytuł oryginału: Meta- and pooled analyses of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk.
Autorzy: Benhamou Simone, Lee Won Jin, Alexandrie Anna-Karin, Boffetta Paolo, Bouchardy Christine, Butkiewicz Dorota, Brockmller Jurgen, Clapper Margie L., Daly Ann, Dolzan Vita, Ford Jean, Gaspari Laura, Haugen Aage, Hirvonen Ari, Husgafvel-Pursiainen Kirsti, Ingelman-Sundberg Magnus, Kalina Ivan, Kihara Masahiro, Kremers Pierre, Le Marchand Loic, London Stephanie J., Nazar-Stewart Valle, Onon-Kihara Masako, Rannug Agneta, Romkes Marjorie, Ryberg David, Seidegard Janeric, Shields Peter, Strange Richard C., Stcker Isabelle, To-Figueras Jordi, Brennan Paul, Taioli Emanuela
Źródło: Carcinogenesis 2002: 23 (8) s.1343-1350, il., tab., bibliogr. 54 poz.
Sygnatura GBL: 312,779

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Susceptibility to lung cancer may in part be attributable to inter-individual variability in metabolic activation or detopxification of tobacco carcinogens. The glutathione S-transferase M1 (GSTM1) genetic polymorphism has been extensively studied in this context; two recent meta-analyses of case-control studies suggested an association between GSTM1 deletion and lung cancer. At least 15 studies have been published after these overviews. We undertook a new meta-analysis to summarize the results of 43 published case-control studies including 18000 individuals. A slight excess of risk of lung cancer for individuals with the GSTM1 null genotype was found (odds ratio (OR) = 1.17, 95 p.c. confidence interval (CI) 1.07 - 1.27). No evidence of publication bias was found (P = 0.4), however, it is not easy to estimate the extent of such bias and we cannot rule out some degree of publications bias in our rsults. A pooled analysis of the original dsata of about 9500 subjects involved in 21 case-control studies from the international Collaborative Study on genetic Dusceptibility to Environmental Carcinogens (GSEC) data set was performed to assess the role of gsTM1 genotype as a modifier of the effect of smoking on lung cancer risk with adequate power. Analyses revealed no evidence of increased risk of lung cancer among carriers of the GSTM1 null genotype (age-, gender- and center-adjusted OR = 1.08, 95 p.c. CI 0.98 - 1.18) and no evidence of interaction between GSTM1 genotype and either smoking status or cumulative tobacco consumption.

416/2523

Tytuł oryginału: Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells.
Autorzy: Baj-Krzyworzeka Monika, Majka Marcin, Pratico Domenico, Ratajczak Janina, Vilaire Gaston, Kijowski Jacek, Reca Ryan, Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
Źródło: Exp. Hematol. 2002: 30 (5) s.450-459, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 305,749

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: objective. peripheral blood plateled-derived microparticles (PMPs) circulate in blood and may interact directly with target cells affecting their various biological functions. methods. To investigate the effect of human PMPs on hematopoiesis, we first phenotyped them for expression of various surface molecules and subsequently studied various biological responses of normal stem/progenitor (CD34+) and more differentiated precursor cells as well as several leukemic cell lines to PMPs. Results. We found that, in addition to platelet-endothelium attachment receptors (CD41, CD61 and CD62), PMPs express G-protein-coupled seven transmembrane-span receptors such as CXCR4 and PAR-1; cytokine receptors including TNF-RI, TNF-RII, and CD95; and ligands such as CD40L and PF-4. Moreover, we found that several of these receptors could be transferred by PMPs to the membranes of normal as well as malignant cells and observed that PMPs: 1) chemoattract these cells, 2) increase their adhesion, proliferation, and survival, and 3) activate in these cells various intracellular signaling cascades including MAPK p42/44, PI-3K-AKT, and STAT proteins. The biological effects of PMPs were only partly reduced by heat inactivation or trypsin digest, indicating that, in addition to the protein components of PMPs, lipid components are also responsible for their biological activity. conclusion. We conclude that PMPs modulate biological functions of hematopoietic cells and postulate that they play an important but as yet not fully understood role in intracellular cross-talk in hematopoiesis...

417/2523

Tytuł oryginału: The effect of verapamil on the antioxidant defence system in diabetic kidney.
Autorzy: Kędziora-Kornatowska K., Szram S., Kornatowski T., Szadujkis-Szadurski L., Kędziora J., Bartosz G.
Źródło: Clin. Chim. Acta 2002: 322 (1/2) s.105-112, tab., bibliogr. 44 poz.
Sygnatura GBL: 303,881

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Bacground: The aim of the study was to examine the effect of verapamil (VP) on lipid peroxidation and activities of key antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px); as well as on glomerular basement membrane (GBM) thickness in streptozotocin-induced diabetic kidney in rats. Methods: Wistar male rats were divided into three groups, 12 rats each: the control (C), diabetic rats (DR), and DR receiving VP, 7 mg/kg body weight in drinking water (DR + VP). Blood glucose (BG) and HbA1c levels, 24-h urinary albumin excretion (UAE) and body weight (BW) were measured every week (0-12 weeks). After 6 and 12 weeks, the animals were sacrificed and malondialdehyde (MDA) content activatives of SOD, CAT and GSH-Px were determined in the kidney homogenate supernatans. Electron micrographs of the glomeruli were scanned and porhphometric investigations were performed by means of a computer image analysis system to compare the glomerular basement basal membrane (GBM) thickness. results: The levels of BG, HbA1c and UAE in DR were significantly higher than in the C group. A progressive increase in the MDA level and a decrease in the SOD, CAT and GSH-Px activaties in the kidney of DR were observed after 6 and 12 weeks. VP administration did not affect BW changes, BG and HbA1 levels in DR. VP decreased lipid peroxidation and augmented the activities of antioxidant enzymes studied in the kidneys of DR as well as decreased kidney weight, GBM thickness and albuminuria in DR. Conclusions: These results confirm the role of oxidative stress in the development of diabetic nephropathy and point to the possible antioxidative mechanism of the nephroprotective action of VP.

418/2523

Tytuł oryginału: Is the predictive power of previous fractures for new spine and non-spine fractures associated with biochemical evidence of altered bone remodelling? The EPOS study.
Autorzy: Vergnaud P., Lunt M., Scheidt-Nave C., Poor G., Gennari C., Hoszowski K., Lopez Vaz A., Reid D. M., Benevolenskaya L., Grazio S., Weber K., Miazgowski T., Stepan J. J., Masaryk P., Galan F., Bruges Armas J., Lorenc R., Havelka S., Perez Cano R., Seibel M., Armbrecht G., Kaptoge S., O'Neill T. W., Silman A. J., Felsenberg D., Reeve J., Delmas P. D.
Źródło: Clin. Chim. Acta 2002: 322 (1/2) s.121-132, tab., bibliogr. 49 poz.
Sygnatura GBL: 303,881

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Bacground: In the European Prospective Osteoporosis Study (EPOS), a past spine fracture increased risk of an incident fracture 3.6 - 12-fold even after adjusting for BMD. We examined the possibility that biochemical marker levels were associated with this unexplained BMD-independent element of fracture risk. Each of 182 cases in EPOS of spine or non-spine fracture that occurred in 3.8 years of follow-up was matched by age, sex and study centre with two randomly assigned never-fractured controls and one case of past fracture. Analysis measured blind were: osteocalcin, bone-specific alkaline phosphatase, total alkaline phosphatase, serum creatinine, calcium, phosphatase and albumin, together with the collagen cross-links degradation products serum CTS and urine CTX. Most subjects also had bone density neasured by DXA. Results: Cases who had recent fractures did not differ in marlker levels from cases who had their had last fracture more than 3 years previously. No statistically significant effect of recent fracture was found for any marker except osteocalcin, which was 17.6 p.c. lower in recent peripheral cases compared to unfractured controls (p 0.05) and this was independent of BMD. Conclusion: Past fracture as a risk indicator for future fracture is not strongly mediated through increased bone turnover.

419/2523

Tytuł oryginału: Magnetic resonance imaging in gynecology - state of the art.
Autorzy: Walecki J., Ceran A., Tarasów E., Panek G.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.111-119, il., bibliogr. 48 poz. - Sympozjum pt. Rozwój metod obrazowania diagnostycznego w położnictwie i ginekologii. Białowieża 11-12.05. 2002
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Dynamic development of MRI and MRS diagnostic capabilities resulted in more frequent application in obstetrics and gynecology; few years back this field was dominated by US. Main advantages of MR include much higher than any other modality spatial and contrast resolution, ability to characterise various tissues and analyse their chemical content. In our paper we would like to share our experience in clinical diagnosis of most common pathologies such as: cervical carcinoma, endometrial caarcinoma, ovarian and vaginal tumors. Techniques provided by MR are specxially useful in gynecological oncology. The greatest challenge which MR faces is staging of neoplasmatic growth and monitoring of treatment. Many additional techniques such as various kinds of multiplanar reconstructions, dynamic examinations, metabolite analysis in MRS greatly increase diagnostic efficiency of this modality.

420/2523

Tytuł oryginału: Tocopherol treatment during chemotherapy in ovarian cancer patients.
Autorzy: Popiela A., Gabryś M., Pańszczyk M., Szewczuk A., Baranowski W.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.121-124, tab., bibliogr. 11 poz. - Sympozjum pt. Nauki przyrodnicze w ginekologii Puławy 31.05-01.06. 2001
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Tocopherol reduces free radicals damage to genomic DNA and regulates cellular metabolism through lowering protein kinase C activity. Tocopherol induces proliferation of the immune system cells through its influence on transcriptional factors. Lack of tocopherol causes dysfunction of cellular immune systems, especially lymphocyte T by destabilisation of cellular membranes. Investigated group consisted of 60 patients with ovarian cancer. Patients received 1000 units of tocopherol per day during postoperative chemotherapy. Tocopherol therapy lowered, although not statistically significantly, morbidity rate, frequency of nausea and vomiting and reduced the intensity of stomatitis, without any influence on total remission number.

421/2523

Tytuł oryginału: Collagen content in the anterior layer of the rectus sheath in postmenopausal women.
Autorzy: Bogusiewicz M., Surowski S., Rzeźniczuk K, Adamiak A., Rechberger T.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.125-128, il., bibliogr. 18 poz. - Sympozjum pt. Nauki przyrodnicze w ginekologii Puławy 31.05-01.06. 2001
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Several studies revealed that postmenopausal decrease in skin thickness and elasticity, uterine prolapse as well as stress urinary incontinence are, at least in part, results of impaired collagen turnover. This study aimed at the evaluation of collagen content in the rectus sheath of premenopausal and postmenopausal women. Samples of the anterior layer of the rectus sheath were taken from 16 premenopausal and 7 postmenopausal women aged 31-51 years and 51-75 years, respectively, undergoing surgery due to benign diseases of the uterus or gall bladder. Collagen was extracted with acetic acid and subsequently with pepsin. The concentartion of collagen (all types) was measured using Sicrol Collagen Assay (Biocolor Ltd., Belfast, N. Irland). Significantly less collagen was extraced from the rectus sheath derived from postmenopausal as compared to premenopausal women (11.0 ń 5.5 (g/gram of wet tissue versus 21.5 ń 10.4 (g/gramof wet tissue; p=0.0033). Moreover, a negative correlation between collagen content and age of the subjects was observed (r = -0.47; p = 0.026). However, when the analysis was carried out separately for each group of women an association between the decrease in collagen content and aage was observed only in women after the menopause. Results of the study indicate that the content ofcollagen in the rectus sheath declines afetr the menopause. This effect seems to be dependant on hormonal changes related to menopause rather than on the process of ageing. The decrease in collagen content may cause faulty wound healing as well as predispose to abdominal hernias.

422/2523

Tytuł oryginału: Influence of LHRH agonistic analog, Decapeptyl, on the balance between proliferation and apoptosis in MCF-7 breast adenocarcinoma cells culture.
Autorzy: Świątecka J., Dzięcioł J., Wołczyński S.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.129-135, tab., bibliogr. 24 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Breast cancer cell line cultures provide an experimental model in which the action of a variety of mitogens and proapoptotic factors can be studied in vitro. Expression of LHRH and its receptor has been demonstarted in human breast adenocarcinoma, MCF-7 cell line, ER-positive. Antiproliferative effect of LHRH-analogs on breast cancer cells has been revealed. The aim of the present study was to examine the effect of different concentrations (10(-6) and 10(-7) g/ml) and time of exposition (24 hr, 48 hr and 72 hr) of Decapeptyl, an agonistic analog of LHRH, on the expression of wild and mutant p553 and bcl-2 gene products as well as on the proliferative activity (expressed as [3H] thymidine incorporation an dProliferating Cell Nuclear Antigen-PCNA and Mitotic Proteins-MPM2 antigens involvement) in MCF-7. Besides we compared the effects of Decapeptyl with the influence of Tamoxifen alone (10 ćM) and Tamoxifen along with Decapeptyl (10 ćM, 10(-6)g/ml respectively). Decapeptyl, independently of the used dose and time of exposition, did not alterradioactivity detected in studied cells in comparison with the control group. Furthermore, label found after 24 hours of exposition was comaprably high as after Estardiol treatment. Thymidine incorporation after Decapeptyl was consistently higher than afetr Tamoxifen and Tamoxifen/Decapeptyl together. Decapeptyl, in dose and time independent manner, do not change the number of PCNA-immunopositive cells, and only at higher concentration [10(-6) g/ml] decreased immunodetection of MPM2...

423/2523

Tytuł oryginału: Treatment of severe hirsutism with gonadotropin-releasing hormone analogues in women with polycystic ovary syndrome and idiopathic hirsutism and its influence on hormonal androgenisation indices.
Autorzy: Kamiński K[azimierz], Łagan J., Cholewa D.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.137-146, il., tab., bibliogr. 25 poz. - Sympozjum pt. Aktualne problemy perinatologii i ginekologii Zabrze - Szczyrk 03-06.04. 2002
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: To compare the therapeutic effect of treatment using either a gonadotropin releasing hormone analogue or an analogue with addition of ethinyl estradiol and cyproterone acetate in women with severe hirsutism with polycystic ovary syndrome and idiopathic hirsutism. Study design: Prospective clinical study. Forty-four hirsute women: 22 with polycystic ovary syndrome and 22 with idiopathic hirsutism were the subject of the study. Each group was divided and received two different treatment schemes. Sixteen women served as controls and received no treatment. After Ferriman-Gallwey scores were obtained, serum levels of testosterone, free testosterone, androsterone, dehyrdroepiandosterone sulfate, luteinizing hormone, insulin, and sex hormone binding globulin were determined. The free androgen index was calculated. Results: Both treatment schemes, using a gonadotropin releasing hormone analogue, and using an analogue with addition of ethinyl estradiol and cyproterone induced significantly the reduction of the free androgen index, and the increase of the sex hormone binding globulin serum levels. Those effects were more distinct in patients who received the analogue alone treatment. The biochemical changes presented in our study were accompanied by significantly decreased mean Ferriman-Gallwey score in the investigated women. Conclusionn(s): We conclude that in the cases with severe hirsutism, the more advantageous treatment is the application of combined therapy using a gonadotropin releasing hormone analogue and a pill containing ethinyl estradiol and cyproterone acetate as compared to the use of gonadotropin releasing hormone analogue alone.

424/2523

Tytuł oryginału: Association between bacterial vaginosis and cervicovaginal flora during pregnancy and threatened preterm labor.
Autorzy: Wasiela M[ałgorzata], Hanke W., Kalinka J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.147-152, il., tab., bibliogr. 39 poz. - Sympozjum pt. Aktualne problemy perinatologii i ginekologii Zabrze - Szczyrk 03-06.04. 2002
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Bacterial vaginosis (BV) in pregnancy may complicate its course and harm the fetus or newborn after vertical transmission. The main aim of this study was to assess an association between bacterial vaginosis and bacterial vaginosis associated flora at early pregancy and threatened preterm labor (TPL). The study population comprised 179 pregnant women attending 10 randomly chosen Outpatients Maternity Units at Ist trimester. Cervicovaginal samples were obtained at 8-16 weeks of gestation. On the basis on Spiegel's criteria Gram-stained vaginal smears were examined for BV and bacterial vaginosis associated flora were sought by culture. In 23 (12.8 p.c.) cases, TPL was diagnosed during pregnancy. Bacterial vaginoses were more frequently diagnosed among women with TPL as compared to women with no symptoms of TPL (39.1 p.c. vs. 26 p.c.). BV increased the risk of TPL (OR=2.14). Women with bacterial vaginosis and TPL were more likely to be culture-positive for bacterial vaginosis associated flora like: M.hominis, U.urealyticum, G.vaginalis, Prevotella spp. Mobiluncus spp. and Bacteroides spp. Bacterial vaginosis at early pregnancy is associated with an increased risk of threatened risk of threatened preterm labor. Detailed microbiological monitoring of all pregnant women at early pregnancy should be introduced.

425/2523

Tytuł oryginału: The effect of oxidized lipoproteins (o-LDL) activity in pregnancies complicated by preeclampsia - role of o-LDL antibodies.
Autorzy: Pawłowicz P[aweł], Stetkiewicz T., Wilczyński J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.153-158, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: The aim of the study is to explain the influence of plasma oxidized lipoproteins on placenta function in pregnancies complicated by preeclampsia and in consequence, on fetal intrauterine asphyxia. Patients and methods: We studied 227 pregnant women between 32th and 40th week of gestation. Study group consisted of 159 pregnant women with preeclampsia. Control group included 118 healthy pregnant women. The level of oxidative stress was estimeted by circulating antibodies anti-O-LDL (oLAB) mesurement in pregnant women plasma. Antibodies anti-O-LDL (oLAB) was measured in plasma using immunoenzymatic method detecting autoantibodies IgG against oxidized plasmatic low density lipoproteins (LDL). Fetal well-being evaluation was based on computer analysis of CTG record (Oxford) and on blood flows measurements in umbilical and middle cerebral arteries of fetus. Results: The values of oLAB levels were higher for the study group than for the control group. Conclusions: In pregnancies complicated by preeclampsia, oxidative stress increase was noted, that index is elevated anti oLDL antibodies (oLAB) concentration.

426/2523

Tytuł oryginału: The influence of the combined, continuous hormone replacement thrapy on the autonomic regulation of gastric myoelectrical activity and heart rate variability.
Autorzy: Banach T., Milewicz T., Krzysiek J., Laskiewicz J., Thor P[iotr] J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.159-167, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Objectives: Presence of dyspepsia symptoms in postmenopausal women during hormonal replacement therapy (HRT) has been often reported by clinicians. It suggests disturbances of motility of upper gastrointestinal tract. Aim: Aim of the study was to evaluate whether the continuous combined HRT (ccHRT) affects the gastric myoelectrical activity. Method: 20 healthy postmenopausal women aged 56 ń 5.5 years, 7 ń 5.4 years after the last menstruaction, were divided into two equal groups: women receiving ccHRT (serum levels of estradiol - 113.3 ń 87.1 ng/l and FSH - 48.1 ń 16.6 IU/l) and a control group (serum levels of estradiol - 23.1 ń 14.8 ng/l and FSH - 73.1 ń 24.3 IU/l). The ccHRT group have received transdermally 17 á-estradiol (Oesclim 50, Fournier) and daily 5 mg of dydrogesterone (Duphaston, Solvay Pharma) orally, for recent 1.4 ń 0.6 years. Thirty min. of electrogastrography (EGG Digitrapper - Synectics, Sweden) and 5 min. of heart rate variability (HRV 2000 - Proster, Poland) recordings before and after 6 min. of SF (sham feeding: 3 chewing gums, 2 min. per each) were carried out in both groups Standard parameters of EGG and HRV were estimated. Statistical analysis: Shapiro-Wilk test, Mann-Whitney u-test, p 0.05 was considered as significant. Results: Mean normogastria with similar period dominant frequencies (PDF) dominanted in both groups before SF (83.9 ń 15.1 p.c. vs. 80.6 ń 27.3 p.c.; p 0.05). SF did not alter frequency but doubled amplitude of basic electrical rhythm (BER) in control group. In HRT patients, normogastria decreased by about 30 p.c. (p 0.05)...

427/2523

Tytuł oryginału: Pretreatment levels of antigens CA 125 and CYFRA 21-1 in patients with endometrial cancer.
Autorzy: Pierzyński P[iotr], Mazurek A., Kuć P., Krętowska M., Mazurek E., Laudański T.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (3) s.169-174, tab., bibliogr. 28 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Cancer antigens CA 125 and CYFRA 21-1 are relatively well studied in certain malignancies such as ovarian and lung cancer, they may also bring some additional information in endometrial cancer. Clinical material comprised of 55 postmenopausal patients with endometrial cancer, serum samples were taken 48 hour before the surgery. CA 125 was assessed immunoenzymatically (reference level 35 U/ml), IRMA (immunoradiometric assay method) was applied for CYFRA 21-1 determinations (reference level 3.2 ng/ml). Cyfra 21-1 levels were elevated in 29.1 p.c. of cases. This marker was positive in 22.1 p.c. of patients of I st FIGO stage and in 56.3 p.c. and 50 p.c. of cases of II and III FIGO stage, respectively. Fisher's exact test and Kruskal-Wallis test confirmed significant differences in CYFRA 21-1 results between groups of FIGO stage of disease. The CA 125 positive cases were of 38.2 p.c. of group studied, this marker was positive in 51.5 p.c. of cases of FIGO I stage of disease, and reached 78.7 p.c. and 100 p.c. cases for stages II and III, respectively. Kruskal-Wallis test confirmed the significance of relationship between CA 125 levels and FIGO stage of disease (p.=0.0025). Our results show that CYFRA 21-1 and CA 125 might be an additional parameter in clinical evaluation of patients with endometrial cancer. Further observation would reveal th einfluence of the CA 125/CYFRA 21-1 positivity on patients' survival, the study concerning that is in the progress.

428/2523

Tytuł oryginału: Persistent oxidative stress in colorectal carcinoma patients.
Autorzy: Gackowski Daniel, Banaszkiewicz Zbigniew, Rozalski Rafał, Jawień Arkadiusz, Oliński Ryszard
Źródło: Int. J. Cancer 2002: 101 (4) s.395-397, tab., bibliogr. 34 poz.
Sygnatura GBL: 305,029

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We examine whether the level of 8-oxo-2'-deoxyguanosine (8-oxodGuo) in lymphocytes DNA is higher in colon cancer when compared to the control group. Factors that may influence oxidative stress such as antioxidant vitamins and uric acid were also determined. Blood samples were obtained from a control group of 55 healthy persons and 43 colon cancers. 8-OxodGuo level and the vitamins concentraction were measured by high-performance liquid chromatography. The levels of 8-oxodGuo were significantly higher whereas the concentractions of the vitamins and uric acid were significantly lower in colon cancer patients than in control group. Therefore, the decreased concentration of antioxidant vitamins together with lower amount of uric acid may be responsible for the formation of pro-oxidative environment in blood of colorectal carcinoma patients.

429/2523

Tytuł oryginału: Effects of pentylenetetrazol-induced kindling of seizures on rat emotional behavior and brain monoaminergic systems.
Autorzy: Szyndler Janusz, Rok Paulina, Maciejak Piotr, Walkowiak Jerzy, Członkowska Agnieszka I., Sienkiewicz-Jarosz Halina, Wisłowska Aleksandra, Zienowicz Małgorzata, Lehner Małgorzata, Bidziński Andrzej, Kostowski Wojciech, Płaźnik Adam
Źródło: Pharmacol. Biochem. Behav. 2002: 73 (4) s.851-861, il., tab., bibliogr. [23] poz.
Sygnatura GBL: 312,498

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The influence of pentylenetetrazol (PTZ)-induced kindling of seizures on the rat emotional behaviour, the brain monoamine turnover rate measured in vitro, and correlation between behavioral and biochemical parameters, were examined in rats. The repeated administration of PTZ (35 mg/kg, ip) evoked kindled seizures in rats (Stage 4 or 5 of clonic - tonic convulsions - maximum). PTZ kindling caused selective changes in the rat emotional behavior, present in some models of anxiety only (a decreased freezing time in the conditioned freezing test and a decreased spontaneous and aversively conditioned ultrasonic vocalization). Simultaneously, PTZ kindling decreased the concentration of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the prefrontal cortex, decreased the DA (HVA/DA ratio) turnover rate in the striatum, and inhibited the serotonin (5-HT) metabolism (5-HIAA/5-HT ratio) in the hippocampus and the prefrontal cortex. Correlations between dopamine (DA) or 5-HT regional metabolic rats in brain structures and animal behavior were either abolished or reversed in PTZ-kindled animals. It is concluded that both DA and 5-HT systems contribute to the emotional effects of PTZ-induced kindling of sizures. The hypothesis is put forward that PTZ kindling-induced inhibition of the serotonergic innervation may lead to the compensatory increase in 5-HT1A receptors in the dentate gyrus of the hippocampus, thus evoking the anxiolytic-like changes in animal behavior.

430/2523

Tytuł oryginału: Temperature-sensitive ovarian carcinoma cell line (OvBH-1).
Autorzy: Bar Julia K., Harłozińska Antonina, Kartarius Sabine, Montenarh Mathias, Wyrodek Ewa, Parkitna Rodriquez Jan M., Kochman Marian, Ożyhar Andrzej
Źródło: Jpn. J. Cancer Res. 2002: 93 (9) s.976-985, il., bibliogr. 65 poz.
Sygnatura GBL: 301,570

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: OvBH-1 cells from a patient with ovarian clear cell carcinoma were established and their biochemical status was analyzed. Cells grown at 37řC exhibited normal cell cycle distribution, whereas the cells shifted to 31řC were arrested in the G2/M phase of the cell cycle. Immunochemical analysis using anti-p53 antibodies (DO-1, PAb240, PAb421, and PAb1620) revealed that only the DO-1 antibody reacted with p53 with a high and similar percentage at both temperatures. PAb240 reacted with a low percentage of cells at 37řC and no reaction was observed at 31řC. PAb421 antibody stained a significantly lower percentage of cells at 37řC than at 31řC. Cells were not stained with PAb1620 antibody and were negative for antibodies against p21**WAF1 and MDM2 proteins independently of the temperature. Sequencing of all coding exons of the p53 gene demonstrated only a neutral genetic polymorphism, i.e. a G-to-A substitution (GAG to GAA) at nucleotide position 13 432. Thus, the observed temperature sensitivity of OvBH-1 cells cannot be ascribed to a p53 primary structure mutation. Based upon immunochemical analyses, we consider, however, that p53 in nuclei of OvBH-1 cells is in a highly unstable conformation. Furthermore, the N-terminal portion of the p53 protein at Ser20 has not been modified, and Lys373 and/or Ser 378 of the C-terminus is acetylated and/or phosphorylated. The nuclear location signal of p53 is preserved. Induction of MDM2 protein is uncoupled from the cell regulatory machinery and the induction of p21**WAF1 by p53 is impaired in OvBH-1 cells.

431/2523

Tytuł oryginału: Związek między aktywnością hydrolaz grzybów wyizolowanych z pochwy kobiet ciężarnych i wybranymi objawami klinicznymi.
Tytuł angielski: Relationship between activity of hydrolases in fungi isolated from vagina of pregnant women and selected symptoms.
Autorzy: Nowakowska Dorota, Kurnatowska Alicja, Wilczyński Jan
Źródło: Wiad. Parazytol. 2002: 48 (3) s.263-269, il., tab., bibliogr. [17] poz., sum.
Sygnatura GBL: 303,658

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Preganancy is considered a factor of vulvovaginal mycosis. Secretion of hydrolases is an important determinantof Candida virulence. Thus, the aim of the study was to found the relationship between activity of 19 hydrolases in fungi isolated from vagina of pregnant women and symptoms of mycosis. 251 pregnant women were examined. Samples were collected from vagina and cultured on Sabouraud media. Activity of hydrolytic enzymes was evaluated using API ZYM (bioMerieux) test. Fungi were found in 20.1 p.c. of vaginal smples. Symptoms were detected in 45.8 p.c. of women. Only 32.1 p.c. of women with discharge and 26.4 p.c. with pruiritus had mycosis. Out of the 19 examined hydorlazes, 13 active enzymes were detected in fungal strains. We found for the first time the relationship betweeen acitivity of gunal esterase lipase and and the presenceof vaginal discharge in pregnant women.

432/2523

Tytuł oryginału: Acta Microbiologica Polonica
Tytuł angielski: Green fluorescent protein as a molecular marker in microbiology.
Autorzy: Rosochacki Stanisław Józef, Matejczyk Marzena
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.205-216, il., tab., bibliogr. s. 214-216
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Molecular markers such as: lacZ(b-galactosidase), xylE (catechol 2,3-dioxygenase), lux (bacterial luciferase), luc (insect luciferase), phoA (alkaline phosphatase), gusA and gur AA (á-glucuronidase), gfp (green fluorescent protein), bla (á-lactamase) and other antibiotic resistance markers, heavy metals resistance genes are commonly used in environmental microorganisms research (Errampalli et al., 1998; Kohler et al., 1999). Most of these markers require one or more substrates, complex media and/or expensive equipment for detection. The gfp gene is widely used as a marker because of its very useful properteis such as high stability, minimal toxicity, non-invasive detection and the ability to generate tha green light without addition of external cofactors and without application of expensive equipment. Various applications of that reporter gene were showed starting from onitoring of microorganism's survival in complex biological systems such as activatedsludge to biodegradation of chemical compounds in soil. GFP allowed the detection, determination of spatial location and enumeration of bacterial cells from diverse environmental samples such as biofilm and water. The gfp as a biomarker was very useful in monitoring of gene expression and protein localisation in bacterial cells, too. The techniques with using gfp marker promise to supply a better understanding of environmental processes. It can make possible to use that knowledge in designing more effective and more efficient methods of biodegradation of toxic compounds from different envirnonments.

433/2523

Tytuł oryginału: Effect of null mutations in dnaK and dnaJ genes on conjugational DNA transfer, proteolysis and novobiocin susceptibility of Escherichia coli.
Autorzy: Modrzewska Magdalena, Karpiński Paweł, Grudniak Anna, Wolska Krystyna I.
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.217-224, il., tab., bibliogr. s. 223-224
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca doświadczalna

Streszczenie angielskie: Escherichia coli null dnaJ and dnaKdnaJ mutants, when introduced to Hfr donor, impair its ability to DNA transfer during conjugation. The additive effect of both mutations was shown. Lack of DnaK and DnaJ chaperones also decreasethe extent of proteolysis in mutant strains. This effect is seen only at 42řC. The influence of double dnaKdnaJ deletion but not single dnaJ deletion on novobiocin susceptibility was also demosntrated.

434/2523

Tytuł oryginału: Ability of Pseudomonas sp. to synthesize aminopeptidases in the presence of various carbon and nitrogen sources.
Autorzy: Jankiewicz Urszula, Bielawski Wiesław
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.247-254, il., bibliogr. [24] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A soil strain of Pseudomonas sp. is able to synthesize at least two aminopeptidases exhibiting high activity in the presence of Phe-á-NA and aAla-á-NA as substrates. Irrespective of the used substrate, total activity of studied enzymes was strongly related to concentrations of organic components (peptone, glutamic acid, glucose) in mineral media and was the higher, the higher the concentration. Tendency of changes in total activity was similar for alanyl- and phenylalanylaminopeptidase though their response to different concentrations of organic components was different. Specific activity measured in the presence of Phe-á-NA and Ala-á-NA as the substrates was not strictly dependent on increasing concentrations of organic commponents in the media. The highest specific activity of aminopeptiidase was obtained in the presene of Phe-á-NA as a substrate on the fifth day of culture in medium containing 1 p.c. glucose. The obtained results seem to indicate the inductive character of the studied aminopeptidases. On the other hand, howevere, they do not eclude other regulatory mechanisms of their synthesis, including catabolic repression.

435/2523

Tytuł oryginału: Primary resistance of Helicobacter pylori to antimicrobial agents in Polish children.
Autorzy: Rożynek Elżbieta, Dzierżanowska-Fangrat Katarzyna, Celińska-Cedro Danuta, Jóźwiak Paulina, Madaliński Kazimierz, Dzierżanowska Danuta
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.255-263, il., tab., bibliogr. s. 262-263
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Helicobacter pylori resistance to antimicrobial agents is an important factor compromising the efficacy of treatment. Therefore the aims of our study were: to determine the prevalence of H. pylori resistance to clarithromycin, metronidazole, amoxycillin and tetracycline in children prior to eradication therapy, to compare different methods of susceptibility testing and to detect mutations responsible for clarithromycin resistance. During 1996-2000, 259 H. pylori strains were isolated from antral gastric biopsies. Susceptibility to antimicrobials was determined by the agar dilution method and the Etest. Mutations in the 23S rRNA gene associated with clarithromycin resistance were analysed by PCR-RFLP and direct sequencing. Overall, ninety-six strains (37 p.c.) were resistant to both drugs. All cultures isolates were sensitive to amoxycillin and only one isolate (0.4 p.c.) was resistant to tetracycline. The agar dilution method and the Etest showed a perfect catergory correlation for clarithromycin and 4 p.c. discrepancies for metronidazole. Primary resistnace to clarithromycin was mainly associated with an A2143G mutation inthe 23S rRNA gene of H. pylori. The study highlight the high prevalence of H. pylori primary resistnace to clarithromycin in Polish children, which implies a need for pretreatment susceptibility testing.

436/2523

Tytuł oryginału: Antimicrobial acitivity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles.
Autorzy: Wolinowska Renata, Zajdel-Dąbrowska Jolanta, Starościak Bohdan J., Kazimierczuk Zygmunt
Źródło: Acta Microbiol. Pol. 2002: 51 (3) s.265-273, il., tab., bibliogr. [16] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Antibacterial and antifungal activity of 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles, including a number of newly obtained derivatives, were examined by diffusion method (inhibition area diameter in solid agar medium) and minimum inhibitory concentration (MIC, in liquid and agar medium). Some of the derivatives tested affected fungal colonymorphology and exerted genotoxic effects in bacteria. Of the tested compounds, 5,6-dichlorosubstituted derivatives appeared the most active against the majority of microorganisms used.

437/2523

Tytuł oryginału: Highlights on the health status of the Polish population in 1999/2000.
Tytuł polski: Najważniejsze informacje o sytuacji zdrowotnej ludności Polski w 1999/2000 roku.
Autorzy: Wojtyniak Bogdan, Goryński Paweł, Seroka Wojciech
Źródło: Prz. Epidemiol. 2002: 56 (1) s.179-192, il., bibliogr. 8 poz., streszcz.
Sygnatura GBL: 301,250

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Celem prezentowanego opracownia jest zwięzłe przedstawienie najważniejszych aspektów aktualnej sytuacji zdrowotnej ludności Polski. W końcu roku 2000 liczba ludności Polski wynosiła 38 644 211, i zmalała o około 9300 w porównaniu do 1999 r. Ostatnie 2 lata to okres gdy populacja uległa zmniejszeniu u około 23 tys. i jest to sytuacja, która wystąpiła po raz pierwszy w powojennej historii Polski. Zaobserwowano także dalszy spadek liczby urodzeń żywych z 382,0 tys. w 1999 r. do 378,3 tys. w 2000 r. oraz zmniejszenie współczynników urodzeń z 9,9 do 9,8 na 1000 ludności. W 2000 r. liczba zgonów była niższa o 13,4 tys. niż w roku poprzednim a współczynnik umieralności ogólnej zmniejszył się z 987 do 952 zgonów na 1000 tys. ludności. Przeciętne dalsze trwanie życia rodzącego się chłopca wynosiło 69,7 lat a dziewczynki 78.0 lat i w porównaniu z 1999 r. dla chłopców jest to więcej o 0,9 roku a dla dziewczynek o 0,5 roku. Przeciętne dalsze trwanie życia mężczyzn w mieście jest dłuższe (70,0 lat) niż na wsi 69,4 lat) podczas gdy u kobiet sytuacja jest odwrotna (77,8 w mieście i 78,4 na wsi). Od 1991 r., gdy niekorzystne trendy w stanie zdrowia ludności Polski zostały odwrócone lub przynajmniej zahamowane, przeciętne dalsze trwanie życia wzrosło o 3,6 roku dla mężczyzn i o 2,7 roku dla kobiet. Od wielu lat główną przyczyną zgonów we Polsce są choroby układu krążenia. W 1999 r. były one odpowiedzialne za 48,7 proc. zgonów (43,6 p.c. u mężczyzn i 54,5 proc. u kobiet). Standaryzowane współczynniki umieralności z powodu chorób układu krążenia malały od 1991r. i w 1999 r. były o 10 proc. niższe niż w 1996 r....

Streszczenie angielskie: In the paper we describe the main problems of the Polish population health status and their trends in the past year. A meaningful improvement in several health parametres can be noticed in Poland since 1991. In the year 2000 life expectancy was 69.7 and 78.0 for males and females respectively - an increase by 3.6 and 2.7 years in comparison with 1991 values. There have been declining trends in cardiovascular mortality, cancer mortality (more often in younger age groups), infant mortality, incidence of infectious disease. Mental health problems are the growing issue. It has been stresses that the pace of progress is far too slow to achieve the level of health indicators in EU countries in the not too distant future.

438/2523

Tytuł oryginału: The rate of eyelid reflex loss following thiopental administration in hypo- and hypergonadism in rabbits.
Autorzy: Szymański Bogdan, Pakulski Cezary, Drobnik Leon, Starczewski Andrzej, Badowicz Beata
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR179-BR183, il., tab., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: The aim of this research was to explain whether different hormonal conditions caused by disturbed concentrations of estrogens and progesterone might lead to alteration of CNS reaction following administration of the hypnotic agent thiopental. The investigated factor was the rate of loss of the eyelid reflx after intravenous thiopental administration, since this corresponds with loss of consciousness. The investigation was performed in 24 sexually mature female Chinchilla rabbaits divided into 4 groups of 6 rabbits each. The animals were oophorectomized (hypoprogesterone/hypoestrogen), hyperestrogen (sham surgery plus estradiol injections), or normal (sham surgery). Twelve weeks later, thiopental (40 mg/ml) was infused through the intravenous cannula at a constant rate (90 ml/hour) until loss of the eyelid reflex, at which time blood was sampled for determination of thiopental, á-estradiol, progesterone and 17ŕ-hydroxyprogesterone concentrations. The ANOVA and Tukey tests were applied in statistical analysis (p = 0.05). Hyperprogesterone rabbits lost consciousness faster (138 ń 34.6 sec), at a lower plasma thiopental concentration (46.3 ń 6.6 ćg/ml), and required less thiopental (24.63 ń 6.44 mg/kg) than controls; hypoprogesterone rabbits lost consciousness slover (207.5 ń 30.9 sec), at a higher plasma thiopental concentration (129.2 ń 24.9 ćg/ml), and required more tiopental (38.51 ń 2.33 mg/kg) than controls. The time of sleep induction in the control group was 190 ń 25.7 sec.; the serum thiopental concentration was 77.8 ń 13.9 ćg/ml, and the total thiopental consumption was 35.8 ń 3.51 mg/kg. Estrogen status has no effect. Various hormonal states are accompanied by different CNS reactions to thiopental. Among the studied groups of sex steroids, only progestins significantly modify the CNS response to barbiturate infusion.

439/2523

Tytuł oryginału: Effect of chronic imipramine or electroconvulsive shock on the expression of mGluR1a and mGluR5a immunoreactivity in rat brain hippocampus.
Autorzy: Śmiałowska M., Szewczyk B., Brański P., Wierońska J. M., Pałucha A., Bajkowska M., Pilc A.
Źródło: Neuropharmacology 2002: 42 (8) s.1016-1023, il., bibliogr. s. 1022-1023
Sygnatura GBL: 305,141

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Previous studied showed that chronic electroconvulsive shock (ECS) or impiramine treatment induced a subsensitivity of group I metabotropic glutamate receptors (mGluR) in hippocampus. In the present study effects of antidepressant treatment on the expression og mGluR1a and mGluR5a, belonging to the group I mGluR, were investigated in rat brain hippocampus using immunohistochemical and Western blot methods, respectively. Male Wistar were treated singly or chronically for 21 days with imipramine, 10 mg/kg, twice daily; with ECS (90 mA, 50 Hz, 0.5 s) every second day; or with haloperidol, 1.2 mg/kg, once daily. Appropriate controls were injected with saline. Rats were sacrificed 24 h after last treatment and their hippocampi were taken out for analysis. It was found that the mGluR1a-immunoreactivity expression increased significantly in Ammon's horn (CA) regions after chronic ECS. The most pronounced effect was observed in the CA3. No significant effects were found after single treatment or after haloperidol. The expression of mGluR5a increased significantly after chronic impiramine in the CA1 and after chronic ECS in the CA3 region. The results obtained indicate an influence of antidepressant treatment on group I mGluR. This increase in the receptor protein level may be a compensatory mechanism developing after chronic treatment.

440/2523

Tytuł oryginału: Neuropharmacology
Tytuł angielski: Synergistic effect of uncompetitive NMDA receptor antagonists and antidepressant drugs in the forced swimming test in rats.
Autorzy: Rogóż Zofia, Skuza Grażyna, Maj Jerzy, Danysz Wojciech
Źródło: Neuropharmacology 2002: 42 (8) s.1024-1030, il., bibliogr. s. 1029-1030
Sygnatura GBL: 305,141

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: In spite of intensive research, the problem of treating antidepressant-resistant depressive patients has not yet been solved. The authors previously reported that combined administration of imipramine and ther uncomperative NMDA receptor antagonist amantadine reduced immobility time in the swimming test in rats to a much greater extent than treatment alone. The present paper investigates the possibility of synergistic interactions between there antidepressants (imipramine, venlafaxine, fluoxetine) with three uncompetitive NMDA receptor antagonists (imatadine, memantine and neramexane). Most combinations resulted in synergistic (hyperadditive) antidepressive-like effects in the forced swim test. Most interesing was the observation that fluoxetine, which was inactive when given alone, showed a positive effect when combined with amantadine (10 and 20 mg/kg), memantine (2.5 and 5 mg/kg) or neramexane (2.5 and 5 mg/kg). The specificyty of these observations is supported by control open field studies, which demonstrated no significant increase, or even a decrease in general locomotion after coadministration of the compounds. The present results suggest that the combination of traditional antidepressant drugs and NMDA receptor antagonists may produce enhanced antidepressive effects, and this is of particular relevance for antidepressant-resistant patients.

441/2523

Tytuł oryginału: Treatment of posterior and anterior urethral trauma.
Autorzy: Dobrowolski Z[ygmunt] F., Węglarz W., Jakubik P., Lipczyński W., Dobrowolska B.
Źródło: Br. J. Urol. 2002: 89 (7) s.752-754, bibliogr. 9 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective. To assess the occurence and treatment of posterior uretheral (most often caused by traffic accidents and comprising half of all cases of urinary tract traumas) and anterior uretheral injury (usually iatrogenic during catheterization or cystoscopy, or caused by blunt trauma, e.g. straddle injury or penetrating trauma). Patients and methods: The records and details of patients with posterior and anterior urethral trauma were analysed from 61 urological depatrments in Poland between 1995 and 1999. Results: During the 5-year period there were 268 cases of posterior and 255 of anterior uretheral injury; for the former, most occurred during traffic accidents, and of the latter 206 were iatrogenic (during catheterization or cystoscopy), of which 48 were accompanied by perineal injury. The posterior injuries were isoplated or involved many organs, mainly pelvic bones. Both types of injuries were diagnosed using ascending urethrography, voiding cysto-urethrography and urethroscopy/endoscopy. Treatment for posterior injuries included cystostomy alone or cystostomy with abdominal drainage or perineal drainage; some patients also had their urethra reconstructed. The treatment for anterior injuries was conservative in 193 patients and surgical in 62. Conclusion: The early detection and appropriate surgical treatment of posterior and anterior urethral injury is crucial for the recovery of urethral function, and avoids many complications.

442/2523

Tytuł oryginału: Comparison of the bladder tumour antigen test with photodynamic diagnosis in patients with pathologically confirmed recurrent superficial urinary bladder tumours.
Autorzy: Lipiński M., Jeromin L.
Źródło: Br. J. Urol. 2002: 89 (7) s.757-759, bibliogr. 20 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: To verify the sensitivity of the bladder tumour antigen (BTAstat, Bard Urological, Convington, GA) test against the sensitive procedure of photodynamic diagnosis (PDD), in which 5-aminolaevulinic acid (5-ALA, a precursor of fluorescent porphyrins) is absorbed by the tumour and detected by ultraviolet cystoscopy, in the early diagnosis of urinary bladder tumours. Patients and methods: Forty-three patients (31 men and 12 women, age range 21-87 years) were assessed after transurethral resection of their bladder tumour using the BTAstat test and PDD. Sixty-nine biopsies from suspect areas of bladder mucosa were taken during cystoscopy under ultraviolet light and all suspect lesions electrocoagulated. Results: Thirty-five patients (81 p.c.) had a positive BTAstat test; in these patients PDD detected malignant lesions (17 Ta1G1-2, two T1G2, two T1G3 and 14 Tis). In eight patients (19 p.c.) the BTAstat was negative but PDD detected three malignant lesions (two Tis and one TaG1). Conclusions: PDD is valuable for detecting bladder malignancy and can identify small lesions not detected by the BTAstat test.

443/2523

Tytuł oryginału: Transurethral microwave thermotherapy: first experiences in Poland.
Autorzy: Jakubczyk T., Stokłosa A[ndrzej], Borkowski A[ndrzej], Habrat W., Dobrowolski Z[ygmunt F.]
Źródło: Br. J. Urol. 2002: 89 (7) s.767-770, tab., bibiliogr. 21 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Objective: To present early experiences in Poland with high-energy transurethral microwave thermotherapy (HeTUMT), considered as a serious alternative to surgery in managing benign prostatic hypertrophy (BPH). Patients and methods: Sixty-one patients were treated in two centres using a commercial TUMT system. The patients were assessed at 5-6 weeks, 3 and 6 months after treatment, evaluating subjective complaints, the International Prostate Symptom Score (IPSS), urinary flow rate and postvoid residual volume. Results: A complete follow-up was available in 44 patients; most had symptomatic improvement 3 months after HeTUMT but it was slightly less pronounced after 6 months. The improvement in objective variables increased up to 6 months after HeTUMT. There was ň 50 p.c. improvement in subjective complaints in 43 p.c. of the patients, in urinary flow rate in 25 p.c. and in residual urine in half. Two patients required re-treatment for a urethral stricture and ineffective HeTUMT. Conclusions: HeTUMT may be a useful treatment for BPH but the outcome for an individual patient seems to be unpredictable. The indications for TUMT should be reviewed individually for each patient.

444/2523

Tytuł oryginału: The efficacy of terazosin for treating benign prostatic hyperplasia: a multicentre clinical trial.
Autorzy: Miękoś E[ugeniusz], Trzepizur Z., Różański W., Boniecki R., Kuśnierz J., Jabłonowski Z., Zydek C.
Źródło: Br. J. Urol. 2002: 89 (7) s.771-772, tab., bibliogr. 7 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

445/2523

Tytuł oryginału: The therapy of benign prostatic hyperplasia using less-invasive procedures: the current situation.
Autorzy: Pomer S., Dobrowolski Z[ygmunt] F.
Źródło: Br. J. Urol. 2002: 89 (7) s.773-775, bibliogr. 14 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

446/2523

Tytuł oryginału: Microangiographic imaging of the prostate.
Autorzy: Słojewski M[arcin], Czerwiński F[lorian], Sikorski A[ndrzej]
Źródło: Br. J. Urol. 2002: 89 (7) s.776-778, il., bibliogr. 11 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: To present the results of microangiography in visualizing prostatic microvascularization. Materials and methiods: A contarst medium was injected into the vessels of prostate specimens obtained during surgery, to fill small vessels; nine specimens were obtained after cystoprostatectomy and two after radical prostatectomy. Branches of prostatic or seminal vesicle arteries were used for the injection, the specimens then fixed in formalin, sectioned and X-rayed. Results: In five samples the quality of the images was sufficient to visualize small arteries; the remaining vessels were partially filled. The vascular map was compared with previous results from historadiographic studies. Conclusions: Microangiographic imaging of prostate specimens provides a unique view of the angioarchitecture of the prostate and correlates closely with historadiographic studies. The correlation with other imaging methods, e.g. Doppler ultrasonography, needs further evaluation.

447/2523

Tytuł oryginału: Usefulness of power Doppler ultarsonography in evaluating erectile dysfunction.
Autorzy: Golubiński A. J., Sikorski A[ndrzej]
Źródło: Br. J. Urol. 2002: 89 (7) s.779-782, il., bibliogr. 16 poz.
Sygnatura GBL: 300,816

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Objective: To evaluate deep penile arterial flow after an intracavernosal injection with papaverine in patients with erectile dysfunction (ED). Patients and methods: Twenty patients with ED were evaluated using power Doppler ultrasonography with a linear probe (8 MHz). Diagnostic tests were undertaken after an intracavernosal injection with 40 mg papaverine. The peak systolic velocity (PSV), enddiastolic velocity (EDV) and resistive index (RI) were analysed. Results: After injecting papaverine, seven patients had a normal erection and appropriate waveform patterns; their mean PSV was 30.7 cm/s, the EDV 4.42 cm/s and the RI 0.85. There was tumescence and elongation of the penis with no rigidity in eight patients; their mean PSV was 23.9 cm/s, the EDV 7.34 cm/s and the RI 0.72. There was no erection in five patients. The abnormal flow values showed insufficient arterial vessels in a quarter of the men, venous leakage in 15 p.c. and mixed ED in 20 p.c. Conclusion: The power Doppler technique allows the accurate location and evaluation of deep penile arteries. Vascular pathology may be differentiated after an intracavernosal injection with a vasomotor agent. Recognising the pathological pattern assists in choosing the best method of treatment.

448/2523

Tytuł oryginału: Brain Research
Tytuł angielski: Expression and kinetic properties of Na+ currents in rat cardiac dorsal root ganglion neurons.
Autorzy: Rola Rafał, Szulczyk Bartłomiej, Szulczyk Paweł, Witkowski Grzegorz
Źródło: Brain Res. 2002: 947 (1) s.67-77, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The expression and properties of voltage-gated Na+ currents in cardiac dorsal root ganglion (DRG) neurons were assessed in this study. Cardiac DRG neurons were labelled by injecting the Fast Blue fluorescent tracer into the pericardium. Recordings were performed from 138 cells. Voltage-dependent Na+ currents were found in 115 neurons. There were 109 neurons in which both tetrodotoxin-sensitive (TTX-S, blocked by 1 ćM of TTX) and tetrodotoxin-resistant (TTX-R, insensitive to 1 ćM of TTX) Na+ currents were present. Five cells expressed TTX-R current only and one cell only the TTX-S current. The kinetic properties of Na+ currents and action potential waveform parameters were measured in neurons with cell membrane capacitance ranging from 15 to 75 pF. The densities of TTX-R (110.0 pA/pF) and TTX-S (126.1 pA/pF) currents were not significantly different. Current threshold was significantly higher for TTX-R (-34 mV) than for TTX-S (-40.4 mV) currents. V1/2 of activation for TTX-S current (-19.6 mV) was significantly more negative than for TTX-R current (-9.2 mV), but k factors did not differ significantly. V1/2 and the k constant for inactivation for TTX-S currents were -35.6 and -5.7 mV, respectively. These values were significantly lower than those recorded for TTX-R current for which V1/2 and k were -62.3 and -7.7 mV, respectively. The action potential threshold was lower, the 10-90 p.c. rise time and potential with were shorter before than after the application of TTX. Based on this we drew the conclusion that action potential recorded before adding tetrodotoxin was mainly...

449/2523

Tytuł oryginału: Effect of cytoprotectants on in vitro drug sensitivity of leukemic cells.
Tytuł polski: Badania in vitro wpływu leków cytoprotekcyjnych na wrażliwość komórek białaczkowych na cytostatyki.
Autorzy: Styczyński J[an], Wysocki M[ichał], Dębski R., Kubicka M., Balwierz W[alentyna], Juraszewska E., Rokicka-Milewska R[oma], Malinowska I., Matysiak M[ichał], Stańczak E., Balcerska A., Płoszyńska A[nna], Kowalczyk J[erzy], Stefaniak J., Małek U., Wachowiak J[acek], Mazur B., Sońta-Jakimczyk D[anuta], Szczepański T., Krawczuk-Rybak M., Chybicka A[licja], Raś M.
Źródło: Pediatr. Prakt. 2002: 10 (2) : Prace nadesłane na Ogólnopolską Konferencję Naukową "Wybrane problemy zdrowotne dzieci i młodzieży u progu XXI wieku" : [sesja naukowa] "Onkologia dziecięca : postępy w diagnostyce i terapii" s.37-40, il., bibiogr. 6 poz., streszcz. - Ogólnopolska konferencja naukowa pt. Wybrane problemy zdrowotne dzieci i młodzieży u progu XXI wieku - sesja naukowa pt. Onkologia dziecięca - postępy w diagnostyce i terapii Poznań 18-20.04. 2002
Sygnatura GBL: 306,430

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie polskie: Celem pracy była ocena wpływu in vitro leków cytoprotekcyjnych: dexrazoxanu (DEX) oraz amifostyny (AMF) na cytotoksyczność daunorubicyny (DNR) i 4-HOO-ifosfamidu (IFO) w linii K562 i w ostrych białaczkach u dzieci. Cytotoksyczność DEX i AMF oraz ich kombinacji z odpowiednimi cytostatykami oceniono w teście MTT w komórkach linii K562 oraz u 18 dzieci z ALL (12 de novo, 6 we wznowie) i 4 z AML de novo. Za miarę cytotoksyczności przyjęto wartość stężenia leku, w którym ginie 50 proc. badanych komórek (LC50). DEX i AMF zmniejszały przeżycie komórek linii K562 odpowiednio o 12 proc. i 20 proc. oraz zwiększały cytotoksyczność DNR i IFO. W stosunku do komórek białaczkowych, zaobserwowano silną korelację pomiędzy wartościami LC50 dla DNR i DNR+DEX (rho = 0,872, p=0,001), natomiast wartości LC50 dla DNR i DNR+DEX nie różniły się (0,50 mg/ml vs 0,55 mg/ml). Również dla IFO i IFO+AMF, stwierdzono korelację pomiędzy wartościami LC50 (rho = 0,64; p=0,01), jednak zaobserwowano tendencję do różnic pomiędzy wartościami LC50 dla IFO oraz dla IFO+AMF (p=0,073, współczynnik regresji liniowej 0,468). Mediana LC50 dla IFO wynosiła 20,23 mg/ml, natomiast dla IFO+AMF była równa 14,47 mg/ml (p=0,623). Przeprowadzone badania wskazują, że AMF może mieć niewielki wpływ cytotoksyczny in vitro na komórki białaczkowe oraz niewielki synergistyczny wpływ na cytotoksyczność IFO, natomiast DEX nie ma wływu na wrażliwość in vitro komórek białaczkowych na DNR.

Streszczenie angielskie: The objective of the study was the analysis of effects of cytoprotectants: dexrazoxane (DEX) and amifostine (AMF) on in vitro drug sensitivity of K562 cells and childhood leukemic cells to daunorubicin (DNR) and 4-HOO-ifosfamide (IFO). Cytotoxicity of DEX and AMF and their combination with respective drugs was analyzed by means of the MTT assay in K562 cell line and and in 18 children with ALL (12 de novo, 6 relapsed) and 4 with AML de novo. Cytotoxicity was expressed by concentration of drug which was lethal to 50 p.c. of tested cells (LC50). DEX and AMF caused cell kill in K562 cell line by 12 p.c. and 20 p.c. respectively and increased cytotoxicity of DNR and IFO. Strong correlation was observed between LC50 values for DNR and combination DNR+DEX (rho = 0,872, p=0,001) in patients samples, while LC50 values did not differ significantly (0,50 mg/ml vs 0,55 mg/ml). The correlation was observed also between LC50 for IFO+AMF (rho = ),64; p=0,01), however tendency to higher resistance to IFO was observed, when compared to combination IFO=AMF (20,23 mg/ml vs 14,47 mg/ml; p=0,623 Mann-Whitney U test; p=0,73 Wilcoxon matched pair test; regression coefficient 0,468). The results suggest weak in vitro cytotoxicity effect of AMF on leukemic cells and its weak synergistic effect on IFO cytotoxicity, while it seems that DEX has no influence on in vitro sensitivity of leukemic cells to DNR.

450/2523

Tytuł oryginału: New approaches to the treatment of pediatric atopic dermatitis.
Tytuł polski: Postępy w leczeniu atopowego zapalenia skóry u dzieci.
Autorzy: Berger William E.
Źródło: Alerg. Astma Immunol. 2002: 7 supl. 2: Alergia, astma immunologia kliniczna: IV Konferencja Środkowo-Europejska: materiały naukowe s.40-48, il. - 4 Konferencja Środkowo-Europejska pt. Alergia, astma, immunologia kliniczna Łódź 12-15.06. 2002
Sygnatura GBL: 313,342

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

451/2523

Tytuł oryginału: Midfacial fractures in children.
Autorzy: Kos M., Łuczak K., Godziński J., Rapala M., Klempous J.
Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.218-225, il., tab., bibliogr. 20 poz., rs., res., Zsfg
Sygnatura GBL: 312,942

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The purpose of the study was to present a modern classification and discuss the treatment of midfacial fractures in children. From the beginning of 1 January 1998 to 31 October 2000, 147 children were treated for different craniofacial fractures. Among them 28 patients (19 p.c.) had extensive midfacial fractures complicated by impaired vision and/or CNS dysfunction and were treated surgically. The fractures were divided into: zygomaticoorbital (1 pt) and zygomatico-orbito-maxillary (5 pts), isolated orbital wall fractures (14 pts) and naso-orbital dislocations (2 pts), upper facial portion dislocations (2 pts) and fronto-naso-orbital (2 pts) or cranio-orbital fractures (2 pts). Clinical examination revealed mainly dysfunction of facial morphology and aesthetics (enophthalmos, telecanthus), CSF leakage, impairment of vision and restricted eyeball movements. Ophthalmological, neurological and radiological (Waters view, CT, NMR) examinations were performed in all cases. The treatment consisted of bone repositioning, reconstruction with autologus bone grafts and three-dimensional stabilisation using titanium plates. Twenty-three pts were operated on before and 5 pts after 7th day after trauma. The restoration of normal facial morphology was achieved in 26 patients. In 2 cases it was imposisible because of the destruction of soft tissue and an extremely extensive bony trauma. Better reposition of bony fragments, prompt healing and better final results were achieved in patients operated before day 7 after trauma. Achieving good results in midfacial fractures requires prompt...

452/2523

Tytuł oryginału: Minimally invasive repair of pectus excavatum - the nuss procedure. A European Multicentre Experience.
Autorzy: Hosie S., Sitkiewicz T., Petersen C., Gbel P., Schaarschmidt K., Till H., Noatnick M., Winiker H., Hagl C., Schmedding A., Waag K.-L.
Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.235-238, il., tab., bibliogr. 5 poz., rs., res., Zsfg
Sygnatura GBL: 312,942

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

ludzie

Streszczenie angielskie: Since the first description in 1998, the minimally invasive reapir of pectus excavatum has gained increasing acceptance. The aim of this survery is to report on the experiences of eight European centres with this technique. 172 patients with a mean age of 15.1 (ń4.6) years were treated and evaluated, 35.5 p.c. were sympomatic. 45.3 p.c. of the patients had an asymmetric configuration of the chest, 74.3 p.c. had a CT index above 3.25. Mean duration of the operative procedure was 76 minutes. Major complications, including dislocation of the bar or stabiliser, pneumonia, atelectasis, local infection, pleural and pericardial effusion and liver injury occurred in 11.1 p.c. of the patients. Minor complications, such as self-resolving pneumothorax, atelectasis and subcutaneous emphysema were reported in another 8.1 p.c. Early cosmetic results were excellent or good in 81.5 p.c. Although the surgical procedure is simple, blood-sparing and short, consideration of some important technical details, proper patient selection and knowledge of the limitations is of vital importance. Long-term results are still lacking.

453/2523

Tytuł oryginału: Perspetives of clinical application of bone morphogenetic proteins in children.
Autorzy: Kos M., Łuczak K., Matusiewicz M., Krzystek-Korpacka M., Rapala M.
Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.272-277, il., bibliogr. 20 poz., rs., res., Zsfg
Sygnatura GBL: 312,942

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The purpose of the study was to modify the method of Bone Morphogenetic Protein (BMP) extraction from bovine bones, estimate the osteoinductive potential of the extracted protein fractions in relation to the degree of protein purity and to test collagen formed in porous discs as a carrier. It was required before BMP application in reconstructive surgery in children. Material and method: BMP preparation from bovine bones was conducted according to a modification of Luyten's method. Three fractions of different degree of purity - demineralised bone matrix "M", extract "E", and fraction after hydroxyapatite column "HP" - were tested for their osteoinductive potential. They were implanted on a collagen carrier into the right rat femoral muscle pouch. Controls were implanted in the same way in the left muscle pouch. The discs were removed after 3 and 6 weeks and tested for alkaline phosphatase activity, a marker of new bone formation. Results: Out of the 10 animals used for testing the osteoinductive potential of matrix fraction, 7 results were positive and 3 negative. In the case of the extract fraction, the analysis was conducted for 3 weeks on 9 rats and 6 weeks on 13 rats. Six and 11 positive results were obtained, respectively. In the HP group 5 animals were observed for 3 weeks (3 positive, 2 negative) and 10 animals for 6 weeks (7 positive, 3 negative). In the case of the extract, the differences in alkaline phosphatase activity after 6 weeks were statistically significant (p ó 0.05) compared to controls. In the other groups, a positive tendency was observed...

454/2523

Tytuł oryginału: Effect of NADH on the redox state of human hemoglobin.
Autorzy: Olek Robert A., Antosiewicz Jędrzej, Caulini Gian Carlo, Falcioni Giancarlo
Źródło: Clin. Chim. Acta 2002: 324 (1/2) s.129-134, il., bibliogr. 15 poz.
Sygnatura GBL: 303,881

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismuatase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced, in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involvews free radical species.

455/2523

Tytuł oryginału: Effect of Helicobacter pylori on delay in ulcer healing induced by aspirin in rats.
Autorzy: Konturek Peter C., Brzozowski Tomasz, Kwiecień Sławomir, Drozdowicz Danuta, Harsch Igor A., Meixner Holger, Stachura Jerzy, Hahn Eckhart G., Konturek Stanisław J.
Źródło: Eur. J. Pharmacol. 2002: 451 (2) s.191-202, il., tab., bibliogr [37] poz.
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs) are major pathogenic factors in peptic ulcer disease but whether these two factors exert synergistic or antagonistic effects on ulcer healing has been a subject of controversy. We compared the effect of aspirin alonne with that of aspirin combined with H. pylori on gastric acid secretion and healing of acetic gastric ulcers in rats. The H. pylori colonization of gastric mucosa was determined by viable H. pylori culture, histology and assessment of bacterial DNA using polymerase chain reaction (PCR). The area of ulcers, gastric blood flow, mucosal generation of prostaglandin E2 and plasma gastrin levels and expression of cyclooxygenase-1, cyclooxygenase-2 and growth factors was determined. Aspirin delayed significantly the healing of chronic gastric ulcers, decreased the gastric blood flow at the ulcer margin and gastric mucosal prostaglandin E2 heneration being without significant influence on gastric acid output H. pylori acquisition that produced moderate gastric inflammation at the ulcer margin delayed significantly the healing of gastric ulcers, decreased significantly both the gastric blood flow at the ulcer margin and the gastric secretion while raising significantly the gastric mucosal prostaglandin E2 generation and plasma gastrin levels. H. pylori infection attenuated the aspirin-induced inhibition of ulcer healing and accompanying fall in the gastric blood flow. Both aspirin and H. pylori up-regulated significantly cyclooxygenase-2 messenger RNA (mRNA)...

456/2523

Tytuł oryginału: Progastrin and cyclooxygenase-2 in colorectal cancer.
Autorzy: Konturek Peter C., Bielański Władysław, Konturek Stanisław J., Hartwich Artur, Pierzchalski Piotr, Gonciarz Maciej, Marlicz Krzysztof, Starzyńska Teresa, Zuchowicz Monika, Darasz Zbigniew, Gtze Jens P., Rehfeld Jens F., Hahn Eckhart G.
Źródło: Dig. Dis. Sci. 2002: 47 (9) s.1984-1991, il., tab., bibliogr. 41 poz.
Sygnatura GBL: 310,037

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Colorectal cancers (CRCs) are one of the most common forms of cancer in Poland and one of the leading causes of dearh. The tumors have been attributed to genetic, dietary, and other environmemtal factors, but recently factors such as gastrin have also been implicated in the carcinogenesis. The relationship between plasma amidated and nonamidated gastrin in CRCs is controversial. This study was designed to determine the plasma levels of progastrin and amidated gastrin in 50 CRC patients before and 3-6 months after removal of the tumor, to determine the tumor concentrations of these gastrin peptides and the level of expression for gastrin mRNA and gastrin/CCKb receptor mRNA, to examine the expression of cyclooxygenase COX-1 and COX-2 in mRNA in CRC tissue, and to compare the prevalence of Hp and its cytotoxic protein, CagA, and cytokines (TNFŕ, IL-1á, and IL-8) in CRCs, before and after removal of tumor. It was found that thwe CRC, its resection margin, and the plasma contained severalfold higher levels of progastrin than of amidated gastrins and that the removal of the CRC tumor resulted in a marked reduction in plasma progastrin level without a significant alteration in plasma levels of amidated gastrins. Both gastrin and CCKb-R mRNA were detected in the cancer tissue and resection margin by RT-PCR, and similary, COX-1 and COX-2 mRNA were expressed in these tissues of moast CRCs. The seroprevalence of Hp, especially that expressing CagA, and levels of IL-1á, but not other cytokines, were significantly higher in CRC patients than in 100 age-...

457/2523

Tytuł oryginału: Multiple MPEP administrations evoke anxiolytic- and antidepressant-like effects in rats.
Autorzy: Pilc A., Kłodzińska A., Brański P., Nowak G., Pałucha A., Szewczyk B., Tatarczyńska E., Chojnacka-Wójcik E., Wierońska J. M.
Źródło: Neuropharmacology 2002: 43 (2) s.181-187, il., tab., bibliogr. s. 186-187
Sygnatura GBL: 305,141

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Several lines of evidence suggest a crucial involvement of glutamate in the mechanism of action of anxiolytic and antidepressant drugs. The involvement of group I mGLu receptors in anxiety and depression has also been proposed. In view of the recent discovery of anxiolytic- or antidepressant-like effects of acute injections of 2-methyl-6-(phenylethyl)-pyridine (MPEP), a selective and brain penetrable mGlu5 receptor antagonist, we designed the present study to examine anxiolytic- and/or antidepressant-like effects of multiple administration of this drug. The anxiolytic-like effects of MPEP were evaluated in rats using the conflict drinking test. The antidepressant-like effect was estimated using the rat olfactory bulbectomy model of depression. Seven subsequent injections of MPEP (1 mg/kg) significantly (by 320 p.c.) increased the number of shock accepted during the experimental session in the Vogel test. MPEP given once daily at a dose of 10 mg/kg, restored the learning deficit of bulbectomized rats after 14 days of treatment, remaining without any effect in the sham-operated animals. N-methyl-D-aspartic acid (NMDA)-induced convulsions in mice were not affected by a single injection of MPEP (30 mg/kg) indicating that at this dose MPEP did not block NMDA receptors. The results indicate that the prolonged blockade of mGlu5 receptors exert anxiolytic- and antidepressant-like effects in rats. No tolerance to anxiolytic-like action occurs. The previously mentioned results further indicate that antagonists of group I mGlu receptors may play a role in the therapy of both anxiety and depression.

458/2523

Tytuł oryginału: Efficacy and safety of TTS fentanyl given directly after tramadol to patients with cancer related pain (based on FEN-POL-2 trial).
Tytuł polski: Ocena efektywności i bezpieczeństwa stosowania TTS fentanylu bezpośrednio po tramadolu, u pacjentów z bólem pochodzenia nowotworowego (na podstawie badania FEN-POL-2 trial).
Autorzy: Łuczak Jacek, Gorzelińska Lidia, Ramlau Rodryg, Wojtukiewicz Marek, Koralewski Piotr, Krzakowski Maciej, Załuski Jerzy
Źródło: Nowotwory 2002: 52 (3) s.216-220, il., tab., bibliogr. 14 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Celem badania była ocena skuteczności i bezpieczeństwa stosowania TTS fentanylu u chorych z bólem nowotworowym, którzy poprzednio nie otrzymywali silnych opioidów. Do badania włączono 72 pacjentów, leczonych przez ostatnie 3 dni traumadolem w dawce 400 mg na dobę z niedostatecznym efektem przeciwbólowym. Natężenie bólu oceniano za pomocą Wizualnej Skali Analogowej (VAS) oraz Słownej Skali Oceny Bólu (VRS). U wszystkich badanych stosowanie TTS fentanylu rozpoczęto od dawki 25 ćg/godz, a w razie wystąpienia bólu przebijającego podawano 5 mg roztworu wodnego szybko działającej morfiny lub nie opioidowe leki przeciwbólowe. Podczas badania dawkę początkową TTS fentanylu utrzymano u 18 chorych, pozostali wymagali zwiększenia dawek. Maksymalna dawka wynosiła 150 ćg/godz. i była stosowana u 2 pacjentów. Trwające 28 dni badanie ukończyło 64 chorych. W ostatnim dniu badania 45/64 (72 proc.) chroych zgłaszało brak bólu lub ból o nieznacznym nasileniu, 15/64 (22 proc.) ból o średnim natężeniu, a 4/64 (6 proc.) ból silny lub bardzo silny wg VRS. W trakcie badania wystąpiły następujące objawy uboczne: nudności (N = 3), wymioty (N = 3), zaparcie stolca (N = 2) i wzmożona potliwość (N = 1), u żadnego z badanych nie pojawiły się objawy depresji oddechowej. TTS fentanyl okazał się skutecznym i bezpiecznym środkiem w leczeniu bólu nowotworowego nie pobierających wcześniej silnych opioidów.

Streszczenie angielskie: The main purpose of the trial was to investigate the clinical efficacy and safety of TTS fentanyl administered over 28 days to patients naive to strong opioids, treated over the past 3 days prior to entering the study with the maximal (400 mg) daily dose of traumadol. Seventy two patients were included in the study, all of them suffering from advanced cancer-related pain. All patients started with the lowerst available dose of fentanyl (Delivery rate 25 ćg/hr. If analgesia was insufficient, the patients were give a strong opiid (5 mg of immediate-release oral morphine) or a non- opioid analgesic as "rescue" medication. Pain intensity was evaluated by means of the Visual Analoque Scale (VAS) and the Verbal Rating Scale (VRS). Sixty four patients were eligible for analysis. During the study 18 patients continued with 25 ćg/hr, and the remaining was administerd a higher doses. The maximum does was 150 ćg/hr (2 patients). At the termination of the trial 45/64 (72 p.c.) patients had no pain or slight pain, 15/564 (22 p.c.) had moderate pain and 4/64 (6 p.c.) experienced strong or very strong pain (VRS). At the end of the trial in 51/64 (79 p.c.) pts pain control was good to excellent (in an overall assessment of pain treatment). Clinically relevant respiratory depression was not observed. The following side effects of opioids were observed: nausea (N = 3), vomiting (N = 3), constipation (N = 2) and sweating (N = 1). Transdermal fentanyl proved to be an effective and safe analgesic in the treatment of cancer-related pain in patients naive to strong opioids.

459/2523

Tytuł oryginału: Papilomavirusines infekcijos paplitimas tarp ligoniu, serganciu gerklu papilomatoze, ir kai kuriu rizikos veiksniu itaka papilomavirusu persistavimui virsutiniuose kvepavimo takuose.
Tytuł angielski: Prevalence of human papillomavirus infection among patients with laryngeal papillomatosis and the influence of some risk factors on the persistence of papillomaviruses in the upper respiratory tract.
Autorzy: Velyvyte Daiva, Laiskonis Alvydas, Uloza Virgilijus, Goździcka-Józefiak Anna
Źródło: Medicina 2002: 38 (5) s.499-504, tab., bibliogr. 19 poz., sum.
Sygnatura GBL: 313,016

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Infection with high-risk human papillomaviruses is a significant risk factor of various benign and malignant human lesions in the upper respiratory tract, skin and the genital tract. The identification of particular human papillomaviruses types is important fir identifying patients with premalignant lesions who are at risk of progression to malignancy. Our aim was to establish the prevalence of human papillomaviruses infection in the upper respiratory tract of patients with laryngeal papillomatosis, to identify viral types, to evaluate the relationship between some risk factors and persistence of human papillomviruses in the upper respiratory tract and to determine the pattern of human papilomaviruses infection. Material and methods. The gorup fo 36 patients with laryngeal papillomatosis and control group of 108 persons without any complains of respiratory system was examined. Epidemiologic characteristics and objective data were analyzed and routine laryngological examination was performed. Pharyngeal swabs of all persons and laryngeal biopsies of 17 patients were taken and analyzed for the presence fo human papillomaviruses DNA. Viral typing using the polymerase chain reaction was performed. Results. Human papillomaviruses DNA was detected in all except one case of laryngeal papillomatosis; then only 23.15 p.c. of persons without complaints of respiratory system were found human papillomaviruses positive. Human papillomaviruses 6, 11 types were predominant (in 88.9 p.c. of patients adn 19.4 p.c. of persons from control gorup). High-risk human papillomaviruses were detected in 52.78 p.c. of laryngeal papillomatosis cases and in 9.26e p.c. of control cases. Risk factors were noted statistically significantly more often in human papillomaviruses positive cases...

460/2523

Tytuł oryginału: Polymorphonuclear leukocyte collagenase as an indicator of the juvenile idiopathic arthritis activity.
Tytuł polski: Kolagenaza leukocytów krwi obwodowej jako wskaźnik aktywności młodzieńczego idiopatycznego zapalenia stawów.
Autorzy: Musiej-Nowakowska Elżbieta, Wojtecka-Łukasik Elżbieta, Maśliński Sławomir
Źródło: Reumatologia 2002: 40 (1) s.5-13, tab., bibliogr. 42 poz., sum.
Sygnatura GBL: 304,900

Hasła klasyfikacyjne GBL:

reumatologia

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: U 58 dzieci chorych na idiopatyczne młodzieńcze zapalenie stawów (mizs.) oceniono zmiany aktywnej i latentnej formy kolagenazy granulocytów obojętnochłonnych (PNM) krwi obwodowej, z uwzględnieniem liczby leukocytów krwi obwodowej, 3 postaci klinicznych mizs., aktywności klinicznej i laboratoryjnej choroby oraz obecności wysięku stawowego u ocenianych chorych. Grupę kontrolną stanowiło 23 zdrowych dzieci. Stwierdzono istotnie statystycznie większą aktywność kolagenazy PNM leukocytów u dzieci z postacią uogólnioną i skąpostawową mizs. w porównaniu z grupą kontrolną. U chorych z postacią wielostawową choroby aktywność kolagenazy PNM leukocytów była niższa niż w grupie kontrolnej, ale różnice nie były statystycznie istotne. Liczba leukocytów krwi obwodowej nie korelowała z aktywnością kolagenazy PNM leukocytów. Wartości kolagenazy latentnej w komórkach krwi tylko chorych z postacią skąpostawową były istotnie wyższe od wartości tego enzymu w grupie kontrolnej. Wykazano istotne powiązanie pomiędzy aktywnością kolagenazy PNM leukocytów a obecnością wysięku stawowego u ocenianych chorych oraz podwyższenie aktywności enzymu u niektórych dzieci, u których rutynowe wskaźniki zapalenia były prawidłowe. Uzyskane wyniki wskazują, że PMN leukocyty dzieci chorych na mizs. są "częściowo" zaaktywowaane już na obwodzie, ale stopień ich aktywacji jest różny w wyodrębnionych podgrupach klinicznych. Aktywność badanego enzymu może być wartościowym wskaźnikiem aktywności choroby, szczególnie postaci skąpostawowej mizs.

Streszczenie angielskie: In 58 children with JIA and 23 age-matched healthly controls the blood leukocyte PMN collagenase was determined. A significant increase in the level of active collagenase was observed in systemic and pauciarticular forms of JIA and a close positive relation was found between the level of both (active and laten) leukocyte collagenase and joint effusion. It is concluded that PMN - leukocytes in systemic and pauciaticular form are already "partially" activated in the peripheral blood and that the level of active leukocyte collagenase may be a valuble index of disease activity, especially in the pauciarticular form of JIA.

461/2523

Tytuł oryginału: The moral and ethical quandry of embryonic stem cell research.
Autorzy: MacQueen Bruce Duncan
Źródło: Med. Sci. Monitor 2002: 8 (5) s.ED1-ED4, bibliogr. 4 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

462/2523

Tytuł oryginału: Proinflammation: A common denominator or initiator of different pathophysiological disease processes.
Autorzy: Esch Tobias, Stefano George B.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.HY1-HY9, il., bibliogr. 85 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Proinflammation is a widespread phenomenon. It has an association with the stress (patho)physiology and is connected with various diseases. Recently, it has been discussed if proinflammation may represent a common (pre)condition in defferent disease states. Evidence for a common proinflammatory pattern in a variety of diseases is analyzed. Proinflammatory (pre)conditions and immune response patterns serve as a common modality in a number of clinically separate diseases. Here, nitric oxide pathways often play a significant role as well. On molecular basis, proinflammation potentially illustrates a common denominator and/or an initiator. Like stress, proinflammation seems to be crucial autoregulatory concept. It normally serves a positive biological goal: Proinflammatory activities, e.g., are initiated to overcome infection or invasion of potentially deleterious biological agents (bacteria, viruses, parasites etc.). While fighting invasion, proinflammation usually shortens biological 'battles' and therefore ameliorates disease-related detrimental or subjectively unpleasing phenomena. However, proinflammation has beneficial and deteriorating capacities and may yet exert detrimental effects. This is especially true, when the fine balance between the different immune response pathways, between anti- and proinflammatory mechanisms, can not be kept. This may occur when a challenge becomes overwhelming or when paterns of a chronic (patho)physiological activity are presented. Thus, proinflammation may represent a relatively unspecific, overlapping/analogous state, underlying various clinical disease manifestations.

463/2523

Tytuł oryginału: Modulation of sickle cell crisis by naturally occurring band 3 specific antibodies - a malaria link.
Autorzy: Kennedy James Randall
Źródło: Med. Sci. Monitor 2002: 8 (5) s.HY10-HY13, bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: This paper's focus is prevention of sickle cell adhesion resulting from the erythrocyte's prematurely denatured hemoglobin. This denatured hemoglobin causes a molecule called band 3 to cluster on the erythrocyte's surface and adhere to the CD36 molecule located on the microvascular endothelium. Natural antibodies recognize these clusters on senescent erythrocytes and prevent their endothelial adhesion and target them for reticuloendothelial elimination. Band 3 is also displayed on the erythrocytes of individuals with falciparum malaria and the vasoocclusive pathology in these patients is prevented in individual with sickle trait. The hypothesis is that prematurely denatured sickle hemoglobin results in an up regulation of natural antibodies which control erythrocyte adhesion in boath malaria and sickle cell disease.

464/2523

Tytuł oryginału: Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14.
Autorzy: Ellingsen Espen A., Morath Siegfried, Flo Trude H., Schromm Andra B., Hartung Thomas, Thiemermann Christoph, Espevik Terje, Golenbock Douglas T., Foster Simon J., Solberg Rigmor, Aasen Ansgar O., Wang Jacob E.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR149-BR156, il., bibliogr. 29 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Pro-inflammatory potential of lipoteichoic acid (LTA) from Staphylococcus aureus is controversial. Present study was undertaken to examine ability of highly purified and characterized S. aureus LTA to stimulate production of pro-inflammatory cytokines in human leukocytes at both mRNA and protein level, and to study involvement of Toll-like receptors (TLRs) adn CD14 in this response. Purified LTA was administered to whole human blood ex-vivo (or primary adherent monocytes) and cytokine response assessed in plasma by EIA. Cytokine mRNA was measured by RT-PCR on leukocyate subsets isolated following stimulation. To study involvement of specific receptors for LTA signaling, CHO cells transfected with CD14 and/or TLR2, TLR4 were used, as well as antibodies directed against these receptors. Addition of highly purified LTA to a whole blood or primary adherent monocytes elicited a time and concentration dependent release of TNF-ŕ, IL-1á, IL-6 and IL-8. mRNA encoding TNF-ŕ, IL-1á, IL-6 and IL-8. mRNA encoding TNF-ŕ, IL-1á and IL-6 seemed to be accumulated in monocytes and T cells, but not in granulocytes and B cells. Expression of TLR2, but not TLR4, in chinese hamster ovary cells conferred responsiveness to LTA. However, antibodies directed towards TLR2 (clone TL2.1) or TLR4 (clone THA125) failed to inhibit TNF-ŕ release induced by LTA in the whole blood model and in adherent monocytes. In contrast, blockade of the CD14 receptor with MAb18D11 strongly attenuated LTA induced release of TNF-ŕ in both models.

465/2523

Tytuł oryginału: Gastric hyperemic response induced bay acid back-diffusion in rat stomachs following barrier disruption - relation to vanilloid type-1 receptors.
Autorzy: Tashima Kimihito, Nakashima Masato, Kagawa Shigeru, Kato Shinichi, Takeuchi Koji
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR157-BR163, il., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Acid back-diffusion activates capsaicin-sensitive sensory neurons (CSN), leading to gastric hyperemic response. We examined the role of vanilloid type-1 receptor (VR1) in gastric hyperemic and ulcerogenic responses in rat stomach following exposure to taurocholate (TC). Under urethane anesthesia, a rat stomach was mounted on an ex-vivo chamber, perfused with 50 mM HCl, and changes in PD, gastric mucosal blood flow (GMBF), and luminal acid loss were measured before and after exposure to 20 mM TC for 30 min, in presence of omeprazole. Capsazepine was co-applied with TC for 30 min to the stomach, while ruthenium red was given i.v. 10 min before TC treatment. TC caused a marked PD reduction, followed by an increase of acid loss and GMBF, resultin in minimal damage in the mucosa. Chemical ablation of CSN attenuated the GMBF response to TC without affecting PD reduction and acid loss, and resulted in severe lesions, while none of these responses induced by TC was significantly affected by either capsazepine or ruthenium red. Intragastric capsaicin increased GMBF, and this response was attenuated by both capsazepine and ruthenium red as well as sensory deafferentation. Both acid back-diffusion and capsaicin increase GMBF mediated by CSN, yet their modes of action differ in terms of capsazepine- or ruthenium red-sensitvity. Although the luminal H+ plays a modulator role for the physiological response mediated by CSN in the stomach, it is unlikely that the action results from the interaction of H+ with the capsazepine- or ruthenium red-sensitive site of VR1.

466/2523

Tytuł oryginału: Endothelin-1 gene polymorphism in the identification of patients at risk for malignant ventricular arrhythmia.
Autorzy: Kozak Milan, Holla-Izakovicowa Lydie, Krivan Lubomir, Vaśku Anna, Sepsi Milan, Semrad Borivoj Semrad, Vacha Jiri
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR164-BR167, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The endothelins are peptides with vasoconstricting and growth-promoting properties. Endthelin-1 (ET-1) is known for its direct positive inotropic and chronotropic effects on isolated heart, and for growth effects. The aim of this pilot study was to investigate the frequency distribution of a common polymorphism of the endothelin (ET-1) gene and its possible relation to the hemodynamic consequences of malignant ventricular arrhythmia in patients with structural heart disease. We studied 26 consecutive patients with malignant ventricular arrhythmia and implantable cardioverter defibrillators (ICD), mean age 62.7 ń 12.2 years, mean LVEF 0.37 ń 0.37 ń 11. The Taq polymorphism of ET-1 was detected using our original PCR method. The PCR product with a length of 358 bp in its non-mutated form contains a target sequence for the TaqI restrictive enzyme, while the mutated product loses this cleavage site. Out of the 26 patients, 9 (34 p.c.) had recurrent palpitations and 8 (30.8 p.c.) had syncopes during their malignant arrhythmic episodes. 19 of the patients were receiving amiodarone after ICD implantation, 7 were not. 15 patients had the (+ +) adn 11 had the (+ -) ET-1 genotype; none had the (- -) genotype. The risk of syncopes was associated with the (+ +) genotype (p = 0.01). Patients with amiodarone had a significantly higher frequency of the (+ +) genotype (p = 0.011). All our results suggested that the presence of the (+ +)ET-1 genotype in patients with structural heart disease, severe left ventricular dysfunction, and malignant ventricular arrhythmia put these patients at a higher risk of hemodynamic collapse during arrhythmic episodes.

467/2523

Tytuł oryginału: Proteinases from Bacillus intermedius secreted in the late stages of sporulation.
Autorzy: Balaban Nelly P., Gabdrakhmanova Leila A., Sharipova Margarita R., Mardanova Aislu M., Sokolova Evgeniya A., Rudenskaya Galina N., Leshchinskaya Inna B.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR168-BR171, il., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Proteinases are widely used in various fields of medicine, such as the treatment of burns, purulent wounds, or decubitus ulcers. On the basis of new microbial proteinases produced by nonpathogenic organisms, a new generation of medical preparations can be developed. Representatives of the Bacillus genera are nonpathogenic and are suitable for producing various proteases in large quantities. B. intermedius is shown to produce a set of alkaline proteases at the early and late stationary phase of growth. The activity of alkaline proteinases was determined using synthetic chromogenous substrates Z-Glu-pNA and Z-Ala-ALa-Leu-pNA. To determine á-galacatosidase aactivity, 2-nitrao-á-D-galactopyranosid was used. Spores were calculated by phase-contrast microscopy. During the late stages of sporulation B. intermedius 3 - 19 cells were shown to secrete two proteinases into the medium: glutamyl endopeptidase, wtih maximum activity at 40 hours of growth, and subtilisin, with maximum activity at 44 hours of growth. Evidence for the secretion of these enzymes into the medium was provided by measuring á-galactosidase activity. Our results show that proteinases from B. intermedius (glutamyl endopeptidase 2 and subtilisin 2) in the late stationary phase of growth are secreted enzymes. This suggests that these enzymes play a role in sporulation.

468/2523

Tytuł oryginału: Is the CD14 receptor gene a marker for smoking dependence?
Autorzy: Hubacek Jaroslv A., Pitha Jan, Skodova Zdena, Poledne Rudolf
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR172-BR174, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: The CD14 receptor is a myeloid cell-specific receptor, which plays a role in host defense and cell stimulation. CD14 positive cells have been detected in lung, and also in brain, and the CD14 receptor is thought to play a role in asthma and allergy reactions. C-159 - T polymorphism in the promoter of the CD14 gene has been associated with myocardial infarction, but not in all studies. Our goal was to establish whether this polymorphism is associated with some of the risk factors of MI. With PCR subsequent restriction analysis we evaluated C-159 - T polymorphism in the CD14 gene in 135 representative selecated male Caucasians. We detected a significantly higher frequency of T/T homozygotes (p 0.025) in subjects who had never smoked (15 of 60, 25.0 p.c.) as compared to smokers and past smokers (6 of 75, 8.0 p.c.). The C-159 - T polymorphism in the CD14 gaene could be a genetic marker associated with smoking dependence, but confirmation in large population study is necessary.

469/2523

Tytuł oryginału: Blood serum levels of soluble forms of adhesion molecules ICAM-1 (sICAM-1) and selectin E (s-selectin E) in patients with various phases of non-Hodgkin's lymphomas.
Autorzy: Wołowiec Dariusz, Frydecka Irena, Kapelko-Słowik Katarzyna, Potoczek Stanisław, Urbaniak-Kujda Donata, Kuliczkowski Kazimierz
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR175-BR178, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Monitoring of disease activity and effectiveness of treatment plays an important role in curative management of patients with non-Hodgkin's lymphomas (n-HL). In the search for markers useful for biochemical monitoring of n-HL patients, a group of proteins called adhesion molecules attracted attention. Particular attention has been devoted to sICAM-1 (intercellular adhesion molecule-1). A correlation between sICAM-1 levels and histological malignancy grades and clinical staging of n-HL, activity of the disease and tumor mass has been observed. The aim of the study was to assess the utility of determining serum levels of selected adhesion molecules in n-HL monitoring. The study was carried out in 60 patients with intermediate and high malignancy grade n-HL according to Working Formulation (anaplastic, lymphoblastic, immunoblastic, centroblastic and centroblastic-centrocytic). Soluble ICAM-1 adhesion molecule (sICAM-1) and selectin E levels were determined in the sera of 31 newly diagnosed patients, 18 with complete remission, and 11 with relapse of the disease. The control group consisted of 15 healthy subjects. The results were expressed in ng/ml. sICAM1 levels were similar in healthy controls and patients in the remission phase and significantly lower in patients with diagnosed active disease and relapses. Relapses were associated with slightly lower levels (on borderline of statistical significance) than of diagnosis Serum selectin E levels, however, demonstrated no differences among patient grups or as compared with controls. The presented results indicate the utility of serum sICAM-1 level determination in assessmetn of acaitivty of intermediate and high malignancy grade non-Hodgkin's lymphomas.

470/2523

Tytuł oryginału: Immunohistochemical investigations of cathepsin D activity in the structures of cholesteatoma.
Autorzy: Chodynicki Stanisław, Chyczewski Lech, Olszewska Ewa
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR184-BR186, il., bibliogr. 12 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Cathepsin D decomposes cytoplasmic proteins, cell organelles, collagen, elastase and proteoglycans. It takes part in angiogenesis and activates osteoclasts, and is thought to play a major role in the destruction of bone tissue by cholestatoma. The aim of the present study was to evaluate the activity of cathepsin D in the structures of cholesteatoma. Cholesteatomas were collected from 16 patients operated on for chronic inflammation of the middle ear. Specimens were fixed in formalin at pH 7.2, after which parrafin slices were made. Cathepsin D was assayed with a Dako set. Keratin was measured by the Kreyberag method. Normal skin from behind the ear was taken from the patients during the same operation. The samples included a stratified, desquamative epithelium (matrix), a streak containing connective tissue (perimatrix), and a mass of keratin debris. Cathepsin D demonstrates high activity in perimatrix cell adjacent to bone tissue, while it occurs in trace amounts in the matrix. A highly positive reaction was observed within keratin, which was present in the superficial layer of the epithelium. Pseudocathepsin located in desquamative epithelial cells demonstrated a high positive reaction. There were trace amounts of cathepsin D within the dermis. In the control group (the skin samples), there were trace amounts of cathepsin D within the corneous layer of the epithelium. Cathepsin D places a major role in bone tissue destruction due cholesteatoma.

471/2523

Tytuł oryginału: Model study of toluene diisocyanate effect on transepithelial ion transport.
Autorzy: Kosik-Bogacka Danuta Izabela, Tyrakowski Tomasz
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR187-BR192, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Żaby

in vitro

Streszczenie angielskie: Toluene disocyanate (TDI), a low molecular weight compound, is commonly known as a factor causing asthma in chemical industry workers. The present study investigated a possible effect of TDI on ion transport using electrophysiological methods aimed at assessment of ion currents occurring in epithelial tissues. The experiemnts were carried out on 119 fragments of isolated frog skin, sampled from 59 specimens of hybrid frog Rana esculenta L. The procedures employed involved transepithelial electrical potential (PD in mV) measuremetn with an Ussing apparatus, modified to enable mechanical stimulation of organs and defined pharmacological actions. Incubation was carried out using Ringer solution and Ringer solution with amiloride and baumetanide. Direct actions of toluene diisocyanate (TDI) were assessed at the time of administering this substance to the Ussing chamber with a peristaltic pump. Based on the model of frog skin tested with Ussing apparatus, administration of TDI to the fluid stimulating preparations incubated with Ringer solution (RH) and with amiloride (AMI) was demonstrated to cause a hyperpolarization invrease after mechanical stimulation. TDI action on isolated frog skin inflicated a change in response to mechanical stimulation, leading to a depolarization. The reaction magnitude of frog skin incubated with bumetanide (BUME) did not chane due to TDI. TDI influences precesses of sodium ion transport in the isolated frog skin model, depending on mechanical stimulation. This indicates that TDI effect on ion transport in epithelial cells depends on C fibres and tachaykinins released from their endings.

472/2523

Tytuł oryginału: Relationship of the BCR gene breakpoint and the type of BCR/ABL transcript to clinical course, prognostic indexes and survival in patients with chronic myeloid leukemia.
Autorzy: Prejzner Witold
Źródło: Med. Sci. Monitor 2002: 8 (5) s.BR193-BR197, il., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Chronic myeloid leukemia is characterized by the presence of the Philadelphia chromosome. At the molecular level a fusion of part of the ABL and BCR genes is observed. The breakpoint locations in the BCR gene fall between exons b2a2 or b3a2 (5' and 3', respectively). Depending on the BCR gene breakpoint two types of mRNA are created. Differences in the types of transcripts and/or the breakpoint site may have an influence on the clinical course of the disease. This prompted the present author to separate subtypes of chronic myeloid leukemia on the molecular level. 71 patients diagnosed with chronic myeloid leukemia in the chronic phase were enrolled in the study. In 61 patients the type of BCR/ABL transcript was determined, and in 27 patients BCR breakpoints were established. Possible correlations between the clinical coaurse, prognostic indexes, survival and the type of transcript and breakpoint were examined. No correlation between the clinical course, prognostic index, or survival was observed in patients with 5' and 3' breakpoints. The patients with b3a2 transcript experienced longer survival than the patients expressing b2a2 transcript. However, no significant differences were observed in the duration of the chronic phase between the two groups. The type of BCR gene breakpoint seems to have no prognostic value in patients with chronic myeloid leukemia. The longer survival of patients expressing the b3a2 transcript may be caused by the less aggressive course of the accelerated or blastic phase.

473/2523

Tytuł oryginału: Apolipoproteins A-1 and B and the likelihood of non-fatal stroke and myocardial infarction - data from The Third National Health and Nutrition Examination Survey.
Autorzy: Qureshi Adnan I., Giles Wayne H., Croft Janet B., Guterman Lee R., Hopkins L. Nelson
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR311-CR316, tab., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The measurement of plasma apolipoproteins (APO) has been proposed for predicting the risk of cardiovascular diseases. However, the association between APOs and stroke is not well defined. We evaluated the association between plasma concentrations of APO A-1 and APO B with a physician diagnosis of stroke (n = 153), and electrocardiogram evidence or physician diagnosis of myocardial infarction (n = 379), in a nationally representative sample of 3,696 US adults aged ň 40 years who participated in the Third National Health and Nutrition Examiantion Survey. Multivariate logistic regression analyses were used to investigate these realtionships. After adjusting for differences in age, gender, race/ethnicity, education, hypertension, cholesterol, body mass index, and cigarette smoking, the upper quartile of APO A-1 (ň 161 mg/dl) when compared with the lowest quartile ( 126 mg/dl) was inversely associated with the likelihood of myocardial infarciton (Odds Ratio [OR], 0.6; 95 p.c. Confidence Interval [CI] 0.4 - 0.9) but not stroke. APO B concentrations were not associated with either myocardial infarction or stroke. However, an APO A-1 to B ratio ň 1.59 when compared wtih a ratio ó 1.04 was associated with a decreased likelihood of myocardial infarction (OR, 0.3; 95 p.c. Cl, 0.2 - 0.6); and stroke (OR 0.4, 95 p.c. Cl, 0.2 - 1.0). Higher APO A-1 concentrations were associate with a decreased likelihood for myocardial infarction but not for stroke. The APO A-1 to B ratio was inversely associated with both myocardial infarction and stroke and may be an important protective clinical marker for atherosclerosis.

474/2523

Tytuł oryginału: Prevalence of significant carotid artery stenosis in patients with transient ischaemic attack.
Autorzy: Rappeport Yael, Simonsen Lene, Christensen Hanne, Boysen Gudrun
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR317-CR320, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Carotid artery stenosis is one of the risk factors for transient ischaemic attack (TIA) and stroke. The purpose of this study was to investigate the prevalence of carotid artery stenosis and the prevalence of candidates for carotid endarterectomy in a hospital-based cohort of TIA patients under 71 years of age. This retrospective study included 205 patients with TIA admitted to an acute stroke unit. A carotid Doppler sonography was performed in 114 patients with TIAs in the internal carotid artery territory and a mean age of 60 years. Based on Doppler examinations, the patients were divided into 4 groups according to the following degrees of stenosis: 40 - 59 p.c., 60 - 79 p.c., 80 - 99 p.c. stenosis, and occlusion. Doppler examinations showed that 5 p.c. had a 40 - 59 p.c. stenosis, 5 p.c. had stenosis 60 p.c. and 4 p.c. had occlusion of the relevant internal carotid artery. Carotid endarterectomy was done in 2 p.c. of the patients. Carotid artery stenosis was associated with a history of angina pectoris and myocardial infarction in univariate analysis, but not in multivariate analysis. The prevalence of significant carotid artery stenosis on the relevant side in patients with TIA was quite low: only 5 p.c. had a degree of stenosis where carotid endarterectomy should be considered. Only 2 p.c. eventually had carotid endarterectomy.

475/2523

Tytuł oryginału: Heart rate response profiles to tilting in healthy and unhealthy neonates.
Autorzy: Massin Martial M., Maeyns Kristel, Lombet Jacques, Rigo Jacques, Gerard Paul
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR321-CR325, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

płeć żeńska

Streszczenie angielskie: Clinical observations suggest the immaturity of the neonate's ability to respond appropriately to cardio-respiratory challenges and tilt test could help in detecating autonomic dysfunction in neonates. Heart rate (HR) responses following a 45ř head-up tilt during sleep were measured within the first week of adjusted age in 7 healthy full-term neonates, 7 healthy preterm infants and 5 neonates with absence syndrome. The tilt and the return to the horizontal position generally provoked a sustained or transient tachycardia, or a flat response in healthy full-term neonates and in preterm neonates, but a sustained or transient bradycardia in neonates with abstinence syndrome. When compared to previous studies in older infants, our data point to the immaturity of the neonate's ability to respond appropriately to cardio-respiratory challenges. No difference is noted between full-term and preterm neonates. The altered HR response in neonates with abstinence syndrome suggests an autonomic dysregulation in this group.

476/2523

Tytuł oryginału: Randomised, cross-over, placebo controlled trial of magnesium citrate in the treatment of chronic persistent leg cramps.
Autorzy: Roffe Christine, Sills Sheila, Crome Peter, Jones Peter
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR326-CR330, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Nocturnal leg cramps are common and distressing. The only treatment of proven effectiveness is quinine, but this has a number of side effects. Magnesium salts have been shown to reduce leg cramp distress in pregnancy. This study tests whether magnesium citrate is effective in the treatment of leg cramps in non-pregnant individuals by conducting in a randomised, double-blind, cross-over placebo-controlled trial. Volunteers suffering regular leg cramps were recruited. Magnesium citrate equivalent to 300 mg magnesium and matching placebo were given for 6 weeks each. The number of cramps recorded in the cramp diary during the final 4 weeks of magnesium and placebo treatment, severity and duration of cramps and the participants' subjective assessment of effectiveness were analysed. In subjects who started with placebo (n = 29) the median (95 p.c. CI) number of cramps was 9 (6-17) on placebo and 5 (4-8) on magnesium. For the group starting with magnesium (n = 17) the median no of cramps was 9 (5-13) on magnesium and 8 (4-14) on placebo. The was no significant carry-over effect (p = 0.88), but a highly significant period effect (p = 0.008). There was a trend towards less cramps on magnesium (p = 0.07). There was no difference in cramp severity and duration between the groups. Significantly more subjects thought that the treatment had helped after magnesium than after placebo 36 (78 p.c.) and 25 (54 p.c.) respectively, (p = 0.03). Diarrhoea was recorded as a side effect of magnesium.

477/2523

Tytuł oryginału: Prevalence of sleep disturbances in Chinese patients with End Stage Renal Failure on maintenance hemodialysis.
Autorzy: Hui David SC, Wong Teresa YH, Li Thomas ST, Ko Fanny WS, Choy Dominic KL, Szeto Cheuk C, Lui Siu F, Li Philip KT
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR331-CR336, tab., bibliogr. 41 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Sleep disorders, such as daytime sleepiness, insomnia, restless legs syndrome (RLS) and obstructive sleep apnea syndrome (OSAS) have been reported as prevalent among patients with end age renal failure (ESRF). As there is little published data from Southeast Asia, a sleep questionnaire was administered to all patients (N = 43, 27 males) on chronic hemodialysis (HD) at the Prince of Wales Hospital, Hong Kong, to assess their sleep complaints. The mean age was 49.5 ń 11.3 years (mean ń SD) with mean body mass index (BMI) of 22.6 ń 3.6 kg/mý. Frequent awakenings and sleep onset insomnia were the most frequent complaints (79 p.c. each), while daytime sleepines occurred in 74 p.c. of patients. Sleep maintenance insomnia and pruritus occurred in 64 p.c. and 60 p.c. of patients respectively. Symptoms of RLS were reported by 70 p.c. of patients. The prevalence OSAS was estimated by the frequency of observed choking (4.7 p.c. of cases), witnessed apnea (14 p.c.), snoring and witnessed apnea (9.3 p.c.), disruptive snoring (1f4 p.c.), disruptive snoring and witnessed apnea (2.3 p.c.), extremely loud snoring (4.7 p.c.). Our questionnaire survey related a hisgh prevalence of sleep complaints such as frequent awakenings, daytime sleepiness, insomnia and RLS in patients with ESRF on maintenance HD, but a relatively low prevalence of OBAS, which may bea related to the low BMI of our ESRF patients.

478/2523

Tytuł oryginału: Lack of association of G-protein subunit gene C825T polymorphism with left ventrucular hypertrophy in essential hypertension.
Autorzy: Shlyakhto Eugene V., Shwartz Eugene I., Nefedova Yulia B., Zukova Anna V., Vinnic Tatyana A., Konrady Alexandra O.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR337-CR340, tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the present study was to determine if there is an association of G-protein á3 subunit (GNB3) gene polymorphisms and left ventricular hypertrophy (LVH) in patients with essential hypertension (EH) in a St. Petersburg population. We examined 135 patients (mean age 48 ń 7 yrs) with mild to moderate EH recruited from the general population of an outpatient hypertension clinic. Left ventricular mass was measured by echocardiography, and the left ventricular mass index (LVM) was calculated. The GNB3 C825T genotype was determined by polymerase chain reaction and restriction digestion. 67 patients (50 p.c.) were homozygous for the C allele (CC), 56 were heterozygous (CT) (41 p.c.) and twelve (9 p.c.) were homozygous for the T allele (TT). THe distribution of genotypes among the patients was in Hardy-Weinberg equilibrium and did not differ significantly when comparing patients with or without LVH. The frequency of the T allele was only slightly higher in patients with LVH (32 p.c.) compared to those without LVH (28 p.c.), NS, and the LVMI was similar in patients with the CC, CT and TT genotypes (122.3 ń 29.8; 118.8 ń 29.9 and 115.2 ń 18.3 g/mý, respectively). No significant discrepancies were found among the various genotype groups in posterior wall thickness, interventricular septum thickness, or functional parameters such as ejection fraction, isovolumetric relaxation time and E/A ratio.

479/2523

Tytuł oryginału: Carboxy terminal domain of the largest subunit of RNA polymerase II of Leishmania donovani has an unusually low number of phophorylation sites.
Autorzy: Dasgupta Arindam, Sharma Shalini, Das Aditi, Sarkar Dwijen, Majumder Hemanta K.
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR341-CR350, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The C-terminal domain (CTD) of the largest subunit of RNA polymerase II in higher eukaryotes has an altered form in Leishmania donovani. To determine whether this is a general feature of the kinetoplastida and to investigate the role of this domain in parasitic RNA pol II transcription, we isolated the gene encoding RNA pol II LS (rpol IILS) and analyzed its C-terminal domain. The discreteness observed may be due to a functional constraint delineating parasite from host. The gene for L. donovani rpol IILS was picked up and sequenced. The CTD of L. donovani rpolIILS was purified as a His-tagged recombinant protein and phosphorylated with a crude kinase extract from L. donovani. An immunoblot analysis of the phosphorylated CTD and photo-crosslinked L. donovani nuclear extracts was done using anti-CTD antibody. The L. donovani rpol IILS is encoded by a single-copy gene. Its transcript is matured posttranscriptionally, with the mini-exon trans-spliced 397 bases upstream of the initiation site. The uniqueness of Leishmania rpol IILS CTD according to prediction analysis was corroborated with in vitro phosphorylation of the recombinant protein. Photoaffinity labelling of L. donovani nuclear run-on transcripts and immunoblot analysis using anti-CTD antibody could identify the active form of RNA polymerase II enzyme in this parasite. The L. donovani rpol IILS possesses a unique C-terminal extension lacking the characteristic repeats but containing serine residues as a potential phosphorylation site. Anti-CTD antiabody could recognize a single molecular species for the RNA pol II enzyme in L. donovani.

480/2523

Tytuł oryginału: QT interval in normal infants during sleep with concurrent evaluation of QT correction formulae.
Autorzy: Benatar Avram, Ramet Jose, Decraene Tine, Vandenplas Yvan
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR351-CR356, il., bibliogr. 29 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

niemowlęta

Streszczenie angielskie: Physiological changes in the QT interval caused by sleep are important in the evaluation of the normal QT interval. Traditionally, Bazett's formula is used for correcting the QT interval for heart rate in childhood. We set out to establish the QT-RR relationship in infants during sleep and concurrently test the performance of four QT correction formulae to determine their comparability and clinical utility. 130 healthy term aborn infants undergoing routine 8-hour polysomnography were enrolled. Continuous EKG limb lead II recording, saturation monitoring, and electroencephalography were conducted. QT intervals and heart rate were measured manually digitally on-screen at hourly intervals. The QT-RR relationship was plotted and QTc calculated using the Bazett, Hodges, Fridericia and Framingham formulae. QTc values for each formula were plotted against age and RR interval. Analysis of variance and regression analysis were used to compare the four formulae and the QT-RR relationship. Mean age was 17.7 weeks (range 4 - 72) and mean weight 6.3 kg (range 2.68 - 12.7). The QT-RR relationship during sleep is curvilinear and appears to be slightly shifted. With increasing heart rate and younger age the Bazett and Hodges formulae overcorrect the QTc whereas the Fridericia and Framingham formulae undercorrect. The Hodges formula correlated best with the RR interval. These formulae are strongly dependent on heart rate. In the setting of a vorderline QTc Bazett prolongation, Hodges formula may be a useful adjunct. Normal infants may have a slightly longer QTc Bazett during sleep.

481/2523

Tytuł oryginału: The impact of very late revascularization of occluded infarct-related artery on cardiac mortality and the incidence of sudden death in survivors of acute myocardial infarction - long-term observation.
Autorzy: Ruta Jan, Bolińska Halina, Ptaszyński Paweł, Rosiak Marcin, Wranicz Krzysztof Jerzy, Kośmider Maciej, Jaszewski Ryszard, Zasłonka Janusz
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR364-CR370, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In survivors of acute myocardial infarction (AMI), an occluded infarct-related artery (IRA) is an important predictor of cardiac death (CD) and sudden death (SD). Early reperfusion of the IRA was associated with improved survival rate. The purpose of the present study was to assess if late IRA revascularization, performed 10 - 30 days after AMI, also has a beneficial effect on the incidence of CD and SD during an 18-month follow-up. The study population consisted of 93 post-MI patients with occluded IRA on coronary angiography. The patients were divided into 2 groups accaording to IRA status at discharge - revascularized (47 patients) or occluded (46 patients) - adn followed. Before revascularization, the two groups of patients did not differ in the prevalence of clinical and angiographic variables, or in the incidence of risk factors for SD. In patients who underwent angioplasty or bypass graft surgery of closed IRA, the markers of electrical instabiality demonstrated no significant improvement after revascularization. During the 18-month follow-up a significantly lower incidence of CD (0 p.c. vs 15 p.c., p 0.01) and SD (0 p.c. vs 11 p.c., p 0.03) was observed in the group of patients with revascularized IRA than in the gorup of patients with occluded IRA. In survivors of AMI, late reperfusion of occluded IRA is associated with reduced 18-month cariac mortality. The beneficial effect of this procedure on the incidence of sudden death, not associated with improvement in myocardial electricall stability, suggests that ischemia can bae considered an important factor modulating the arrythmogenic substrate.

482/2523

Tytuł oryginału: Post-traumatic plasminogenesis in intraarticular exudate in the knee joint.
Autorzy: Rość Danuta, Powierza Wojciech, Zastawna Ewa, Drewniak Wanda, Michalski Arkadiusz, Kotschy Maria
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR371-CR378, il., tab., bibliogr. 35 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Intraarticular exudate, observed in the knee joint a few weeks after a traumatic incident, is on of post-traumatic complications. Evacuation of the exudate prevents damage of the articular cartilage bay the fluid possessing potent proteolytic properties. In view of the lack of data concerning the role of the fibrinolysis system in proteolytic destructive processes induced by the exudate, the aim of the study was to find out whether plasmin is present in the post-plasmatic intraarticular exudate and whether it is generated in connection with excessive plasminogen activator levels or deficiency of plasminogen activator inhibitors in the synovial fluid. 55 patients (mean age 356. l), with history of knee joint traumas and exudate appearing in the joint at least 3 weeks post-traumatically. The patients and 30 healthy controls were subjected to the following tests of the synovial fluid and plasma: the levels of tissue plasminogen activator, type 1 plasminogen activator inhibitor, plasmin-alpha2-antiplasmin complexes, fibrinogen and von Willebrand factor antigen, the activity of urokinase plasminogen activator, prekalikrein, alpha-2-antiplasmin, C1 inhibitor, type 1 plasminogen activator inhibitor, euglobulin clot lysis time. The patient's plasma demonstrated significantly higher levels of fibrinogen and PAP complexes, and decreased u-PA activity in comparison in that obtained from the controls. Significantly higher levels of PAP complexes and u-PA antigen were found in the exudate than in the patients' plasma. Local, intraarticular plasmin generation, probably u-PA-dependent, occurs in knee joint exudate. Plasminogen activator inhibitors are probable not very closely associated with increased intraarticular plasminogenesis.

483/2523

Tytuł oryginału: Hepatitis B vaccine immunogenicity in patients with chronic HCV infection at one year follow-up: the effect of interferon-alpha therapy.
Autorzy: Chlabicz Sławomir, Grzeszczuk Anna, Łapiński Tadeusz Wojciech
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR379-CR383, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Patients with chronic hepatitis C are at risk of additional liver damage when infected with other hepatotropic viruses, and should be vaccinated against HAV and HBV. However, the optimal dose and schedule are still being debated. In this study we assessed hepatitis B vaccine immunogenicity in patients with chronic hepatitis C at one-year follow-up. The effect of interferon alpha treatment on vaccine safety and anti-HBs response was also evaluated. 48 hepatitis C patients were enrolled, of whom 12 were on interferon treatment. Recombinant HBV vaccine was administered on a 0-1-6 month schedule, and anti-HBs titers were measured at months 1, 2, 7, and 18. The overall seroprotection rate at month 7 was 72.9 p.c. in hepatitis C patients, compared to 90.9 p.c. in the controls. Subjects with chronic hepatitis C had significantly lower percentages of good responders: 50 p.c. versus 90.9 p.c. At month 18, only 34.1 p.c. of the HCV patients had seroprotective titers of anti-HBs, compared to 90 p.c. in the control gorup. At all study points there were no significant differences in seroconversion rates and seroprotection rates between subgroups of hepatitic C patients receiving interferon or those without antiviral treatment. Hepatitis C is associated with decreased HBV vaccine response. We suggest that postvaccination anti-HBs testing should be performed in all hepatitis C patients, and additional vaccine doses should be provided to those without protection. Alternatively, higher doses of vaccine (40 ćg) may be considered. Treatment with interferon was safe and well tolerated.

484/2523

Tytuł oryginału: Flu-like response on statins.
Autorzy: Sinzinger Helmut
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR384-CR388, tab., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Stains became one of the most widely used family of drugs meanwhile. Myopathy in various forms is among the most prevalent side effects. Only few attention has been paid to its various forms of appearance. We are reporting on a so far unknown flu-like response on statins with very severe symptoms of exhaustion, wekness, paining muscles and joints elevated acute phase response markers and sometimes even subfebrile body temperature. The 5 patients (4 males, 1 female) we monitored showed this response to some but not all the available statins with an onset of latest 3 weeks after strating therapy. Symptoms appeared already at the lowest available dose of the respective statin. Only in 3 of the patients, CK-elevation was noted, while 4 of them exahibited a severe increase in 8-epiprostaglandin-F2a, an in-vivo oxidation injury marker. Latest 5 weeks after statin withdrawal the symptoms completely disappeared. Liver function parameters remained normal in all the patients at all time. Misinterpretation of this flu-like response is particularly dangerous as the treatment with antibiotics initiated in one of the patients may have had deleterious consequence. The pathophysiological background of this response remains to be asessed.

485/2523

Tytuł oryginału: The Prozac boom and its placebogenic counterpart - a culturally fashioned phenomenon.
Autorzy: Slingsby Brian Taylor
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR389-CR393, bibliogr. 46 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The placebo effect describes the mind-body process of altering the body's physiological state as well as the brain's neurological state. Catalyzed by the mind's believing in a certain drug, ritual, and/or treatment, it generally referes to the phenomenon of an ill individual recovering to health. Subsequent to the Prozac boom (fluoxetine), which occurred during the 1980s and 90s in the United States, a new form of placebogenic phenomena was defined: 'cosmetic psychopharmacology', whereby the healthy individual is transformed to fit better in his/her cultural medium (defined by one's values and beliefs). In order to discover how cultural metaphors (e.g. media-generated symbolism, social ideals) influence psychopharmacological treatment, this report investigates the following questions: Is the Prozac Boom culturally fashioned? Is cosmetic psychopharmacology culturally fashioned? Are cosmetic psychopharmacology and its ability to better the individual (relative to American ideals) a placebogenic phenomenon? The data and speculation for the present report was obtained via a cultural and ciritcal review. It would appear that (1) the Prozac Boom and subsequently its by product, cosmetic psychopharmacology, are culturally fashioned; (F2) cosmetic psychopharmacology is considered to result from one's belief in a drug and thus is placebo-influenced. Media-generated symbolism, which represents and reciprocally fashions the ideals and values of that society, influence the social perceptions of a drug's effectiveness and the quality of a particular treatment. In the clinical setting, the infuential roel of these preconceived notions abaout a certain treatment and/or drug need to be further considere and studied.

486/2523

Tytuł oryginału: Infertility status of male individuals with abnormal spermiogram evaluated by cytogenetic analysis and in vitro sperm penetration assay.
Autorzy: Wiland Ewa, Wojda Alina, Kamieniczna Marzena, Szczygieł Monika, Kurpisz Maciej
Źródło: Med. Sci. Monitor 2002: 8 (5) s.CR394-CR400, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The possibility of a link between altered sperm morphology and functional ability, possibly reflected on the genetic level, is still a matter of controversy. 60 infertile males with pathological spermiogram and 14 healthy individuals with confirmed in vivo fertility were selected for our study. Ejaculated sperm was assessed according to the standard criteria set by the World Health Organization, which constituted a basis for subgroup classification. The sperm penetration assay (SPA) using human sperm and hamster oocytes was subsequently performed with concomitant cytogenetic analysis of peripheral blood leukocytes (PBL). More than 55 p.c. of infertile males with pathological spermiogram exhibited a decreased ability for penetration of the oocyte (SPA ó 30 p.c.). The lowest penetration ability was observed in the semen of patients with oligoasthenoteratozoospermia (OAT), of whom as many as 75 p.c. presented negative SPA values. We noted only 1 case of chromosome aberration (Robertsonian translocation) in the analyzed cohort of infertile patients. Strikingly, in the identified group of infertile patients with chromosome structural polymorphism (acrocentric chromosomes), we revealed a statistically significant decreased rate of sperm - oocyt penetration. A causative link may exist between sperm malfunction and a specific feature of the chromosome variants detected in the blood leukocytes of infertile males with pathological spermiogram.

487/2523

Tytuł oryginału: Effect of combined dopamine and diltiazem on renal function after cardiac surgery.
Autorzy: Yavuz Senol, Ayabakan Nurcan, Goncu M. Tugrul, Ozdemir I. Ayhan
Źródło: Med. Sci. Monitor 2002: 8 (5) s.PI45-PI60, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In order to evaluate the effect of the combined use of dopamine and diltiazem on renal function in patients undergoing coronary artery bypass grafting (CABG), we conducted a prospective randomized study. Sixty patients (8F, 52M) with normal preoperative renal function undergoing CABG were divided randomly into four groups, including control (n = 15), use of dopamine-only (n = 15), use of diltiazem-only (n = 15), and combined use of dopamine and diltiazem (n = 15). The patients ranged in age from 38 to 74 years (mean 59.3). Drug administration (diltizem: intravenous infusion of 2 ćg kg-1.min-1, dopamine: 2 ćgúkg-1.min-1) was initiated 24 hours before surgery and was continued for 72 hours. Creatinine and osmotic clearances were found to be significantly higher in the combined group compared with the other groups 24 hours after surgery (p 0.05). There was no significant difference in respect to creatinine clearance, osmotic clearance, and free water clearance in the dopamine-only and diltiazem-only groups compared to the control group. The free water clearance at 24 and 72 hours after surgery were significantly higher in the combined group compared with the other groups (p 0.05). In the control group, dopamine-only, and diltiazem-only groups á2-microglobulin was statistically higher 72 hours postoperatiave than preoperatively (p 0.050). There were no significant changes in á2-microglobulin levels in the combined group. The combined use of dopamine and diltiazem may provide a positive contribution and sufficient protection for early postoperative renal function in patients undergoing CABG.

488/2523

Tytuł oryginału: Digital image analysis system for the quantification of infiltrates and cell adhesion molecules in inflammatory cardiomyopathy.
Autorzy: Noutsias Michel, Pauschinger Matthias, Ostermann Karsten, Escher Felicitas, Blohm Jan-Henrik, Schultheiss Heinz-Peter, Khl Uwe
Źródło: Med. Sci. Monitor 2002: 8 (5) s.MT59-MT71, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We attempted to develop a digital image analysis (DIA) system for endomyocardial biopsies (EMBs) to reliably quantify a) biopsy quality, b) immunohistochemically-marked infiltrates, and c) cell adhesion molecules (CAMs) in relation to net heart area (HA) for the semi-automated diagnosis of inflammatory cardiomyopathy (InfCM). 140 EMBs from dilated cardiomyopathy (DCM) patients and 14 autopsy heart samples (controls) were immunostained for T-lymphocytes (CD2, CD3, CD4, CD8), á2-integrin + infiltrates (CD18, LFA-1, Mac-1) and CAMs (immunoglobulin superfamily: ICAM-1, HLA class I, HLA DR, VCAM-1, CD58; selectins: CD62E and CD62P; and the á1-integrin chain CD29). EMB quality was assessed visually on a three-point scale. Infiltrates were quantified visually (per hpf) and by DIA (per mm2 HA). CAM expression was evaluated semiquantitatively and bay DIA (area fraction [AF]: stained area relative to HA). DIA-evaluated HA correlated significantly with the visual assessment of EMB quality. The visual evaluation of both infiltrates and CAMs correlated significantly with the respective DIA-based quantification. DIA-quantified CAM-AF and infiltrates were discriminated bay the CAM clssification (CAMs+: n = 87; 62 p.c.) compared to controls. DIA-quantified cCAM immunoreacativity correlated significantly with the DIA-quantified counter-receptor + infiltrates. DIA evaluation of biopsy quality, infiltrates, and CAMs was devoid of inter- and intraobserver variability. The DIA system presented here enables standardized and observer-independent assessment of EMB quality and intramyocardial...

489/2523

Tytuł oryginału: Significance of ultrasound vaginal cervicometry in predicting preterm delivery.
Autorzy: Mara Michal, Calda Pavel, Haakova Lucie, Zizka Zdenek, Dohnalova Anna, Zivny Jaroslav
Źródło: Med. Sci. Monitor 2002: 8 (5) s.MT72-MT77, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The objective of the study was to verify whether ultrasound vaginal cervicometry, performed in the 18th - 20th week of gestation, can effectively predict preterm delivery. 279 singleton pregnancies were prospectively studied from the middle of gestation until delivery. In the 18th - 20th week of gestation we performed ultrasound vaginal cervicometry, which we used to determine the length of the cervix, judge the shape of the internal os, and evaluate the cerviacometry as normal or abnormal on the basis of these parameters. With the aid of onedimensional and multi-dimensional analysis, we tested the dependence of completed weeks of gestation and preterm delivery on the results of ultrasound cervicometry. 247 women completed the study. Cervicometry was evaluated as abnormal in 53 women (21.46 p.c.). We proved a significant dependence of delivery prior to the 34th week of gestation on cervical length (p 0.01), abnormal shape of the internal os (p 0.0001; RR = 10.35), and abnormal cervicometric result (p 0.0001; RR = 29.28). Delivery prior to the 37th week was also significantly dependent on all observed parameters. Of the individual cervicometric parameters, cervical length had the most significant impact on the completed weeks of gestation. The implementation of ultrasound vaginal cervicometry in the prenatal screening program can lead to effective and early selection of women with a significantly increased risk of preterm delivery.

490/2523

Tytuł oryginału: Embryonic stem cell research: the relevance of ethics in the progress of science.
Autorzy: Ruiz-Canela Miguel
Źródło: Med. Sci. Monitor 2002: 8 (5) s.SR21-SR26, il., bibliogr. 50 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płód

Streszczenie angielskie: Experimentation with embryonic stem (ES) cells has become an important breakthrough in medical research. However, it is also a source of controversy, because it requires the destruction of the human embryos used to derive ES cells. This paper deals with some of the ethical issues concerning ES cell research. To begin with, the terms used in the debate on the wthical status of the human embryo need to be defined. Apart form the presumed benefits of ES cell research, we should also consider such issues as the strong opposition to this reseach by a large part of society, who argue in favour of protecting and respecting human embryos; the fragility and defenseless of human embryos; and the contradiction in terms inherent in the statement that human embryos must be treated with respect. Secondly, we should focus on possible conflicts between the financial, scentific, and ethical aspects of this debate. Thirdly, the significance of social and political debate requires clear and complete information that takes all consequences into account. Finally, the paper suggests how multipotent adult stem cell research may be an optimal and realistic alternative to ES cell research.

491/2523

Tytuł oryginału: Stress in cardiovascular diseases.
Autorzy: Esch Tobias, Stefano George B., Fricchione Gregory L., Benson Herbert
Źródło: Med. Sci. Monitor 2002: 8 (5) s.RA93-RA101, il., bibliogr. 85 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Evidence for a connection between stress and selected cardiovascular diseases is analyzed. Does stress cause or exacerbate cardiovascular diseases? The stress phenomenon is illustrated and the impact of stress on the circulatory system is examined. In particular, the pathophysiological significance of stress in hypertension, atherosclerosis, coronary artery disease, myocardial infarction (and others) is described. Stress plays a major role in various (pathophysiological processes associated with the circulatory system. Thereby, it potentially has ameliorating or detrimental capacities. However, with regard to cardiovascular disease, stress most often is related to deleterious results. The specific outcome depends on multiple variables (amount of stress, duration of is influence, patient's history/predisposition, genetic components - as they all may after functions of the basic stress response components: the hypothalmic-pituitary-adrenal axis and the sympathoadrenal medullary system). Stress has a major impact upon the circulatory system. It plays a significant role in susceptibility, progess, and outcome of cardiovascular diseases. Subjective or individual differences have also to abe taken into account. Stress, especially 'adequate'acute stress - stress that is not 'overwhelming' - may improve performance and thus be beneficial in certain cases. The close relationship between stress and cardiovascular diseases may represent an important aspect of modern medicine. New therapeutic strategies have to be set in place.

492/2523

Tytuł oryginału: Coronary artery calcification and carotid intima-media thickness in ischaemic heart disease.
Tytuł polski: Choroba niedokrwienna serca. Wskaźnik uwapnienia tętnic wieńcowych i grubość kompleksu intima-media.
Autorzy: Kabłak-Ziembicka Anna, Pasowicz Mieczysław, Konieczyńska Małgorzata, Przewłocki Tadeusz, Klimeczek Piotr, Pieculewicz Monika, Tracz Wiesława
Opracowanie edytorskie: Szwed Hanna (koment.), Pruszyński Bogdan (koment.).
Źródło: Kardiol. Pol. 2002: 57 (8) s.83-95, il., tab. - Tekst. równol. w jęz. pol.
Sygnatura GBL: 313,397

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background. Coronary angiography is the reference standard in detection of coronary artery disease (CAD). Exercise testing and stress echocardiography have been used for selection of patients who should undergo coronary angiography, however, sensitivity and specifity of these methods are limited. Recently, new non-invasive methods such as multidetector spiral computed tomography (MSCT) and carotid artery ultrasonography have been introduced in the evaluation of CAD. Aim. To examine the usefulness of coronary calcium scoring (CS) and carotid intima-media thickness (IMT) in the assessment of the extent of CAD. Methods. The study group consisted of 100 patients (mean age 59 ń 9.4 years, range 43-79 years) with a clinical diagnosis of CAD. All patients underwent coronary angiography as well as carotid Doppler ultrasoung for IMT measurement and MSCT for coronary artery calcification evaluation. Results. Significant positive correlations between age and CS as well as IMT values were found (p=0.0001, r=0.385; and p=0.0000, r=0.486, respectively). The IMT values significantly correlated with the number of coronary lesions and were significantly higher in patients with two-vessel or three-vessel CAD than in patients with normal coronaryy angiography (p=0.0530 and p = 0.0009, respectively). All patients with the CS values 400 had significant CAD, including 56 p.c. of patients with a three-vessel CAD There was a positive correlation between IMT and CS (p=0.0000, r=0.506). Sensitivity and specifity of IMT and CS detecting CAD were 84 p.c. an 58.8 p.c. for for IMT, and 57 p.c. and 100 p.c. for CS, respectively.

493/2523

Tytuł oryginału: Coronary flow reserve measured by stress transesophageal echocardiography in hypertrophic cardiomyopathy with subvalvular gradient.
Tytuł polski: Ocena rezerwy przepływu wieńcowego przy pomocy echokardiografii przezprzepływowej u chorych z kardiomiopatią przerostową z gradientem podzastawkowym.
Autorzy: Nemes Attila, Forster Tamas, Palinkas Attila, Hgye Marta, Csanady Miklos
Źródło: Kardiol. Pol. 2002: 57 (8) s.96-101, tab., bibliogr. 34 poz., streszcz. - 23 Kongres Europejskiego Towarzystwa Kardiologicznego Sztokholm 2001
Sygnatura GBL: 313,397

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wstęp. Rezerwa przepływu wieńcowego (CRF) jest zmniejszona u niektórych chorych z kardiomiopatią przerostową (HCM). Jedną z nowszych nieinwazyjnych metod pozwalających na ocenę CRF jest echokardiografia przezprzełykowa. Cel. Porównanie wielkości CRF u chorych z HCM w zależności od występowania gradientu podzastawkowego. Metodyka. Prędkość przepływu rozkurczowego była mierzona w warunkach podstawowych i po badaniu leku rozkurczającego naczynia wieńcowe - dipyridamolu. Lek podawano w dawce 0.56 mg/kg w ciągu 4 minut, a szczytowa prędkość przepływu mierzona po 6 minutach CRF obliczano jako stosunek prędkości szczytowego i podstawowego przepływu rozkurczowego. W badaniu wzięło udział 19 chorych z HCM oraz 24 chorych z prawidłowym wynikiem koronarografii, stanowiących grupę kontrolną. Wyniki: U wszystkich chorych z HCM przerost dotyczył przegrody międzykomorowej (średnia grubość 20.7 ń 3.7 mm). U 6 chorych obserwowano skurczowy ruch przedniego płatka zastawki mitralnej, a gradient podzastawkowy wynosił średnio 38.7 ń 16.3 mmHg (zakres od 27 do 67 mmHg). U 3 chorych nie rejestrowano prędkości skurczowego przepływu wieńcowego w warunkach podstawowych, a jedynie po podaniu dipyridamolu. U pozostałych 3 chorych z tej grupy rejestrowano wstęczny przepływ skurczowy, który nie zmieniał sięe po podaniu leku. Średnia wartość CRF u chorych z grupy kontrolnej z prawidłowym wynikiem koronarografii wynosiła 3.08 ń 1.08, a u chorych z HCM - 2.14 ń 0.68 (P 0.001). Masa lewej komory ...

Streszczenie angielskie: Bacground. Coronary flow reserve (CFR) is decreased in some patients with hypertrophic cardiomyopathy (HCM) and may be determined by means of stress transesophageal echocardiography. Aim. To compare the CRF in HCM patients with or without a subvalvular gradient. Methods. Diastolic velocities were measured under baseline conditions and during maximum stress. Dipyridamole was administered as a vasodilator agent in a dose of 0.56 mg/kg over 4 minutes, and peak velocities were measured after 6 minutes. The CRF was calculated as the ratio of the baseline diastolic velocites. Nineteen HCM patients were examined, together with 24 patients with normal coronary angiograms who severed as controls. Results. In all HCM patients, the hypertrophy was studied in the intraventricular septum (mean thickness 20.7 ń 3.7 mm). In 6 patients, systolic anterior motion was observed, and subvalvular gradients were measured (38.7 ń 16.3 mm Hg, range 27-67 mmHg). In 3 patients no systolic velocites were detected under baseline conditions, but only during maximum stress. In another 3 patients there was a reserved systolic flow, which remained unchanged during stress. The CRF in patients with normal coronary angiograms was 3.08 ń 1.08, while in HCM patients - 2.14 ń 0.68 (p 0.001). The left vemntricular mass was significantly higher in the HCM patients than in controls (410.7 ń 112.8 g vs 264 ń 69.4 g, p 0.05). There was a slight dfifference in the CRF between HCM patients with or without a ...

494/2523

Tytuł oryginału: Surowce zielarskie w zewnętrznym leczeniu skóry.
Tytuł angielski: Plant raw materials in external skin therapy.
Autorzy: Lutomski Jerzy
Źródło: Wiad. Zielar. 2002: 44 (7) s.4-6, tab.
Sygnatura GBL: 311,671

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

495/2523

Tytuł oryginału: Critical overview of the current status of organ donors with primary central nervous system tumors.
Autorzy: Hałoń Agnieszka, Patrzałek Dariusz, Rabczyński Jerzy
Źródło: Ann. Transplant. 2002: 7 (1) s.6-14, tab., bibliogr. 34 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The transmission of donor-related malignancies by organ transplantation is rare event but biological behaviour of malignant tumors developed by the transplanted patients is in general more agressive than similar ones in non-transplanted population. This paper presents an analysis of our series of cases and a review of the literature to the point of the transmission of cancer from organ donors with primary central nervous system tumors. Patients with primary CNS neoplasms have been accepted for organ donation because these tumors very rarely spread outside the CNS. To our knowledge, after an extensive review of the literature, the CNS tumor transmission risk with transplantation may be estimated between a little more than 0 p.c. and 3 p.c. In the light of available data and in accordance with our investigations we consider that patients with CNS tumors can be accepted as donors as long as the risk of dying on the waiting lists is significantly higher than the tumor transferral risk. Organ donors with benign or low-grade CNS tumor should be accepted unreservedly. Donors with high-grade tumors should be consider as "marginal donors" and their assessment can be based on the comparison and the balance between the risk of tumor transmission and the medical condition of the recipient.

496/2523

Tytuł oryginału: Organ donor with cerebellar medulloblastoma: a case report and review of the literature.
Autorzy: Hałoń Agnieszka, Patrzałek Dariusz, Rabczyński Jerzy
Źródło: Ann. Transplant. 2002: 7 (1) s.15-19, tab., bibliogr. 28 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Transplantation of organs and tissues procured from brain-dead organ donor (BDOD) is nowadays a common and desirable therapy for end-stag organ failure, especially kidneys, liver and heart. The majority of organs for transplantation are obtained from individuals with massive and irreversible central nervous system injury. This group of organ donors is however unsatisfactory and small according to needs. Significant percentage of multiorgan donors could be found among patients suffering from primary cerebral neoplasms as these rarely metastasize spontaneously outside the central nervous system. The paper presents the case of a 30-year-old male donor with a central nervous system tumor. Histogenesis, pathway of malignant dissemintation, prognosis and the use of organ donor with cerebellar medulloblastoma are also discussed.

497/2523

Tytuł oryginału: Aneurysm of common and internal iliac arteries 14 years after kidney transplantation - a case report.
Autorzy: Gałązka Zbigniew, Świercz Paweł, Rongies Witold, Szmidt Jacek
Źródło: Ann. Transplant. 2002: 7 (1) s.22-24, il., bibliogr. 15 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of the report is to present successful surgical treatment of aneurysm of common iliac artery (CIA) and internal iliac artery (IIA) in a kidney transplant patient. The aneurysm of CIA and IIA, causing arterial hypertension, was diagnosed and treated surgically in a 47-year-old male patient, who had received a cadaveric kidney transplant 14 years before. During the operation, consisting of aorto-femoral unilateral grafting with transplanted kidney artery implantation to the side of the graft and aneurysm exclusion, cold Ringer's lactate perfusion was used to protect the transplanted kidney. There were no postoperative complications; the transplant function is satisfactory in a follow-up of 44 months. Exclusion of CIA and IIA aneurysm using aorto-femoral unilateral graft is an effective way of treatment in kidney transplant patients. The cold perfusion during aortic cross-clamping is an effective and simple method of transplanted kidney protection.

498/2523

Tytuł oryginału: From the guest editor.
Autorzy: Myśliwiec Michał
Źródło: Ann. Transplant. 2002: 7 (1) s.27, bibliogr. 3 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

499/2523

Tytuł oryginału: Thrombotic microangiopathy in renal transplantation.
Autorzy: Chiurchiu Carlos, Ruggenenti Piero, Remuzzi Giuseppe
Źródło: Ann. Transplant. 2002: 7 (1) s.28-33, tab., bibliogr. 76 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The term thrombotic microangiopathy (TMA) encompasses syndromes of thrombocytopenia, microangiopathic haemolytic anaemia, neurologic deficits, renal dysfunction and variable signs of organ impairment. Childhood cases of TMA with predominant renal failure are usually referred as Haemolytic Uremic Syndrome (HUS), and adult cases with major neurological involvement as Thrombotic Thrombocytophenic Purpura (TTP). Exotoxins, produced in most cases by E. Coli O157:H7, have been related to diarrhea associated HUS(D+HUS). Anticancer (mitomycin), immunosuppressive drugs (cyclosporin, tacrolimus and OKT3) and as well as some antiplatelet agents (ticlopidine, clopidrogel) have been associated with both HUS and TTP. Defective factor H or vWF protese activity have been found familiar and recurrent forms. Endothelial damage and dysfunction is most likely the initial event of the pathogenic process that eventually leads to plateled aggregation, microvascular thrombosis and tissue ischemia. TMA may occur de novo in the native kidneys of patients who received a non-kidney transplant or in the transplanted kidney patients who progressed to ESRD because of a disease other than HUS. Calcineurin inhibitors and vascular rejection are most often involved in these cases. The disease may also recur on the transplanted kidney in patients who progressed to ESRD because of HUS/TTP. The risk of postransplant recurrence is negligible for D + HUS but is close to 100 p.c. in familial/recurrent forms associated with low C3 and decreased factor H bioavailability or activity...

500/2523

Tytuł oryginału: Fibrinolysis in chronic renal failure, dialysis and renal transplantation.
Autorzy: Opatrny K., Zemanova P., Opatrna S., Vit L.
Źródło: Ann. Transplant. 2002: 7 (1) s.34-43, il., bibliogr. 70 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The best known function of the fibrinolytic system is its ability to dissolve blood clots. The key enzyme of fibrinolysis, plasmin, is formed by conversion from plasminogen through the action of activators, the most important of which is tissue type plasminogen activator (tPA). Low levels of tPA or excessive levels of plasminogen activator inhibitor-I (PAI-I) cause hypofibrinolysis, causally related to the development of atherosclerosis and associated thrombotic complications, as well as with the development of venous and arterial thrombosis. A chronic decrease in renal function leads to hypofibrinolysis due primary to low levels of tPA. Hypofibrinolysis is present both in patients treated by long-term hemodialysis and by peritoneal dialysis. The hemodialysis procedure acutely raises the plasma levels of tPA, primarily as a result of the bioincompatibility of materials in the extracorporeal circuit. In peritoneal dialysis, dialysis solution dwell time is associated with an increase in PAI-I levels in the abdominal cavity. Fibrinolysis defects occur also in renal transplant recipients. In transplant patients, the main abnormality is also hypofibrinolysis which, however, unlike the situation with the other methods of renal replacement therapy, is secondary to a rise in PAI-I. A role in the increase of the plasma levels of PAI-I in transplant patients is played by steroid-and cyclosporine-based immunosuppression, most likely by metabolic disorders such as insulin resistance or dyslipoproteinemia, and by genetic factors. Animal experiments ...

501/2523

Tytuł oryginału: The effect of chronic allograft rejection on plasma regulators of fibrinolysis.
Autorzy: Perkowska A., Elhasade As., Durlik M., Placha G., Gałązka Z., Lao M[ieczysław], Gaciong Z[bigniew]
Źródło: Ann. Transplant. 2002: 7 (1) s.44-51, tab., bibliogr. 40 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Chronic renal allograft rejection is often associated with the presence of fibrin thrombi in the microcirculation. Our purpose was to evaluate the influence of chronic rejection on fibrinolytic regulators in plasma of renal allograft recipients. We evaluated the concentration and activities of tPA, uPA and PAI-I in plasma from kidney allograft recipients. We studied 64 patients who underwent kidney transplantation from cadaveric allograft donors. At the time of the study 38 patients had stable graft function for at least 6 months proceeding the study, and 26 recipients had biopsy-proven chronic rejection of the kidney transplant. Control group included 30 healthy blood donors. In kidney transplant recipients we found significantly higher plasma tPA activity (median: 0.99 IU/ml; range 0-3.8 IU/ml) in comparison to healthy controls (median: 0.15 IU/ml; range: 0-2.8 IU/ml) (p = 0.002) as well as significantly lower plasma PAI-I activity (median: 7.06 U/ml; range 0-33.2 U/ml) in comparison to healthy controls (median: 21.8 U/ml; range: 0-36.7 U/ml), (p = 0,0001). Among transplant recipients, PAI-I plasma activity in recipients with chronic graft rejection (median: 10.16 U/ml; range 0-33.2 U/ml) was significantly higher than in patients with stable graft function (median: 4.83 U/ml; range: 0-22.9 U/ml). In transplant recipients with stable graft function and poorly controlled hypertension we found significantly higher PAI-I plasma activity in comparison to recipients with normal blood pressure (p=0.006). In kidney transplant recipients there was a positive correlation between...

502/2523

Tytuł oryginału: Effects of fluvastatin on homocysteine and serum lipids in kidney allograft recipients.
Autorzy: Małyszko Jolanta, Małyszko Jacek S., Brzósko Szymon, Pawlak Krystyna, Myśliwiec Michał
Źródło: Ann. Transplant. 2002: 7 (1) s.52-54, il., bibliogr. 18 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Hyperhomocysteinemia is now recognized as an independent risk factor for atherosclerotic cardiovascular disease in patients with normal renal function. Hyperhomocysteinemia is common in patients with chronic renal failure. Kidney transplant recipients have a high risk of cardiovascular death. Recently, attention has been paid to the association between homocysteine and cardiovascular disease. Dyslipidemia is also common in kidney transplant recipients. The purpose of this study was to assess whether fluvastatin in a dose of 20 mg affects homocysteine concentration in 10 stable renal transplant recipients. We evaluated Hcy, lipoprotein (a) by the use of commercially available kits as well as plasma fibrinogen and cholesterol, triglycerides and albumin levels. All the parameters were studied before and after 1,2 and 3 months of fluvastatin treatment. Cholesterol and LDL decreased significantly as early as after I month and remained lowered during the therapy. No significant changes in Hcy, lipoprotein (a) and fibrinogen were found during therapy with fluvastatin. Fluvastatin is an effective hypolipemic agent and has no effect on Hcy and fibrinogen concentration in kidney tranplant recipients.

503/2523

Tytuł oryginału: Effects of immunosuppressive drugs on platelet aggregation in vitro.
Autorzy: Małyszko Jolanta, Małyszko Jacek S., Takada Akikazu, Myśliwiec Michał
Źródło: Ann. Transplant. 2002: 7 (1) s.55-68, il., bibliogr. 40 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Disturbances in platelet functions are found in kidney diseases and may contribute to the progression of atherosclerosis with its thrombotic complications. Kidney allograft recipients are particularly prone to dyslipidemia and have a high risk of cardiovascular death. The purpose of this work was to assess effects of various immunosuppressive drugs on aggregation of platelets obtained from healthy volunteers and chronically hemodialyzed patients. Platelet aggregation in the whole blood and in PRP was induced by collagen (2-ćg/ml whole blood and PRP) arachidonic acid (0.75 mM whole blood and PRP). ADP (10 ćM-whole blood and 5 ćM-PRP), ristocetin (0.75 mg/ml - whole blood and 1.5 mg/ml - PRP) and was studied after preincubation with clinically relevant concentrations of cyclosporine A, FK 506, 15-deoxyspergualin, azathioprine, mizoribine, mycophenolic acid and mycophenolate mofetil. Preincubation with cyclosporine A resulted in a significant increase in platelet aggtegation, whereas preincubation with FK 506, azathioprine, mizoribine, mycophenolic acid and mycophenolate mofetil caused a decrease in platelet aggregation. 15-deoxyspergualin did not affect platelet aggregation. Enhanced plateled aggregation. Enhanced platelet aggregation in CSA-treated kidney allograft recipients may have clinical implications in regard to the reported tendency to thrombosis in those patients, and to CSA-induced nephrotoxicity. Thus, inhibition of platelet activity in these patients might be of clinical benefit.

504/2523

Tytuł oryginału: Chronic treatments with haloperidol and clozapine alter the level of NMDA-R1 mRNA in the rat brain: an in situ hybridization study.
Autorzy: Ossowska Krystyna, Pietraszek Małgorzata, Wardas Jadwiga, Dziedzicka-Wasylewska Marta, Nowicka Dorota, Wolfarth Stanisław
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.1-9, il., bibliogr. 45 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The aim of the present study was to examine the influence of 3-month administration of the typical neuroleptic haloperidol (1 mg/kg/day) and the atypical one clozapine (30 mg/kg/day) on the expression of the NMDA-R1 mRNA in different brain structures using in situ hybridization in rats. A long-term treatment with haloperidol decreased the NMDA-R1 mRNA level in intermediate and caudal parts of the caudate-putamen and in more caudally localized regions of parietal and frontal cortices, but increased it in the CA1 region of the hippocampus. No significant changes in the nucleus accumbens, insular cortex, CA3 and dentate gyrus of the hippocampus were found after haloperidol administration. Clozapine did not influence the NMDA-R1 mRNA expression in the hippocampus, as well as in the intermediate and caudal regions of the caudate-putamen, but significantly increased it in the rostral region of the latter structure, in the nucleus accumbens and insular cortex. The present study suggests that both these neuroleptics influence the expression of the mRNA of the NMDA-R1 subunit in brain structures which are thought to be important for development of psychotic symptoms.

505/2523

Tytuł oryginału: Effect of (S)-3,5-DHPG on learning, exploratory activity and anxiety in rats with experimental hypoxia.
Autorzy: Nadlewska Agnieszka, Car Halina, Oksztel Robert, Wiśniewski Konstanty
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.11-18, il., bibliogr. 38 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: We investigated the effects of (S)-3,5-DHPG, a selective agonist of group I metabotropic glutamate receptors (I mGluRs), on certain behaviors in rats after short-term hypoxia as a model of experimentally induced amnesia. The effect of (S)-3,5-DHPG administered intracerebroventriculary (icv) at doses of 0.01, 0.1 and 1.0 nmol was assessed using behavioral tests: the open field test, the passive avoidance response and the elevated "plus" maze test. (S)-3,5-DHPG did not change the number of crossed fileds and rearings and only at a dose of 0.01 nmol it increased the number of bar approaches in the open field test. (S)-3,5-DHPG used at all doses improved consolidation, and at doses of 0.01 and 1.0 nmol it improved retrieval in the passive avoidance test. (S)-3,5-DHPG did not produce any significant effects in control rats in the elevated "plus" maze test. Hypoxia inhibited locomotor and exploratory activity of rats, significantly impaired consolidation and retrieval processes. We observed tendency to shortening the time spent in open arms and to decrease in the number of entries into open arms in the number of entries into open arms in the elevated "plus" maze in rats with undrewent hypoxia. In hypoxia-treated groups of rats, (S)-3,5-DHPG inhibited locomotor and exploratory activity in comparison with the control groups administered (S)-3,5-DHPG. Hypoxia significantly inhibited beneficial effects of (S)-3,5-DHPG on consolidation and retrieval in passive avoidance. (S)-3,5-DHPG only at the dose of 1.0 nmol used before hypoxia improved consolidation and at the dose of 0.01 nmolenhanced retieval in comparison with saline-treated group subjected to hypoxia...

506/2523

Tytuł oryginału: Role of noradrenergic system in the mechanism of action of endogenous neurotoxin 1,2,3,4-tetrahydroisoquinoline: biochemical and functional studies.
Autorzy: Michaluk Jerzy, Krygowska-Wajs Anna, Karolewicz Beata, Antkiewicz-Michaluk Lucyna
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.19-25, il., tab., bibliogr. 43 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: It is well recognized that 1,2,3,4-tetrahydroisoquinoline (TIQ) is a substance capable of inducing in animals a syndrome, regarded as an animal model of Parkinson's disease. This study was designed to evaluate the effect of the endogenous neruotoxin TIQ on the brain noradrenaline (NA) metabolism in mice and on an arterial blood pressure in rats. It was shown for the first time that TIQ significantly increased NA metabolism, induced NA release and raised the level of its final metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG), in mouse brain. The comparative biochemical studies using specific agonist (clonidine) and antagonist (yohimbine) of ŕ2-adrenergic receptors ligands have shown that observed biochemical effects were similar to those produced by ŕ2-adrenergic antagonist, yohimbine. In functional studies, the systolic and diastolic blood pressure was measured using a non-invasive blood pressure transducer. Both acute and multiple treatment with TIQ produced a strong hypotensive effect, haveing decreased both systolic and diastoic blood pressure in rats. Development of tolerance to the hypotensive effect was observed after multiple treatment with TIQ. The data coming from these experimental studies apparently suggest an important role of the noradrenergic system in the mechanism of action of endogenous compounds from TIQ group. The results may also support the hypothesis assuming a causal relationship between noradrenergic dennervation, activity of the nigrostriatal dopamine system, and some clinical manifestation of Parkinson's disease.

507/2523

Tytuł oryginału: Role of nitric oxide in benzodiazepines-induced antinociception in mice.
Autorzy: Talarek Sylwia, Fidecka Sylwia
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.27-34, il., bibliogr. 39 poz., sum.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The influence of nitric oxide (NO) on antinociceptive acivity of diazepam (DZ), chlordiazepoxide (CDP) and clonazepam (CZ) was examined using the writhing test in mice. The effect of DZ was also studied in mice using hot plate and tail flick tests. DZ (1.25, 2.5 and 5 mg/kg), CDP (1.25, 2.5, 5, 10 and 20 mg/kg) and CZ (0.075, 0.3125, 0.625, 1.25 and 2.5 mg/kg) produced significant, dose-dependent (DZ CDP) antinociception in mice. The benzodiazepines (BZs)-induced antinociception was antagonized by flumazenil (5 mg/kg) and was not changed by naloxone (2.5, 5 and 10 mg/kg), except that of CZ, which was reversed by 5 mg/kg of naloxone. N**G-nitro-L-arginine methyl ester hydrochloride (L-NAME) as well as 7-nitroinadzole (7-NI) intensified antinociceptive activity of BZs. The antinociceptive effect resulting from co-administration of L-NAME with CZ and 7-NI with CDP was reversed by L-arginine. Methylene blue (MB) increased, whereas L-arginine (but not D-arginine) decreased antinociceptive effects of the studied BZs. These results suggest that the NO-cGMP pathway is involved in the mechanism of BZs-induced antinociception in the writhing test in mice.

508/2523

Tytuł oryginału: Effects of tibolone on the development of osteopenia induced by ovariectomy in rats.
Autorzy: Pytlik Maria
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.35-43, tab., bibliogr. 32 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Experimental osteopenia caused by ovariectomy in rats may reflect postmenopausal bone changes, which are the effect of osteogen deficiency. The aim of the present study was to investigate the effects of tibolone (0.25 mg/kg/day and 2.5 mg/kg po) administered for 4 weeks on the development of osteopenia caused by bilateral ovariectomy in 3-month-old female Wistar rats. The experiments were carried out on six groups of animals: I(C) - control sham operated rats, II (OVX) - ovariectomized rats, III (OVX+T-0.25) - ovariectomized rats which were administered tibolone at a dose of 0.25 mg/kg, IV (OVX+T-2.5) - ovariectomized rats which were administered tibolone at a dose of 2.5 mg/kg, V (T-0.25) - sham operated rats which were administered tibolone at a dose of 0.25 mg/kg, VI (T-2.5) - sham operated rats which were administered tibolone at a dose of 2.5 mg/kg. The following parameters were examined in all the groups: body weight gain, bone mass, length and diameter, mineral and calcium contents in the tibia and femur, endosteal and periosteal transverse growth, endosteal and periosteal osteoid width, transverse cross-section area of the cortical diaphysis and that of the marrow cavity in the tibia, epiphyseal cartilage width, trabeculae width in the epiphysis and metaphysis of the femur. Mechanical properties of the femur were also studied. Bilateral ovariectomy induced osteopenic skeletal changes in mature female rats. Tibolone (0.25 mg/kg/day and 2.5 mg/kg/day po) administered to ovariectomized rats for 28 days decreased the development of osteopenic skeletal changes induced by bilateral ovariectomy.

509/2523

Tytuł oryginału: In vitro cytostatic activity of 8-substituted and tricyclic analogues of acyclovir.
Autorzy: Hładoń Bogusław, Gośliński Tomasz, Laskowska Halina, Baranowski Daniel, Ostrowski Tomasz, Zeidler Joanna, Ruszkowski Piotr, Golankiewicz Bożenna
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.45-53, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Out of a series of twenty 8-substituted or/and 1,N-2-bridged (tricyclic) derivatives of acyclovir (a selective antiherpetic drug), known to be nontoxic to normal cells, seven compounds were found to exhibit moderate cytostatic activity in KB human tumor tissue culture system with ED50 activity values ranging from 0.052-0.094 x 10**-3 mole/l. The structure-activity relationship analysis indicated that the primary factors determining their cytotoxicity were: 1) bromine atom at the C-8 position of the bicyclic derivatives and 2) unsubstituted appended ring in the tricyclic derivatives. Combination of two structural elements carrying the cytotoxicity gave diverse effects, enhancement or decrease in activity depending on particular cases. Two compounds (of four selected), 8-bromoacyclovir and 1,N-2-ethenoacyclovir, having unsubstituted 9-[(2-hydroxyethoxy)methyl] chain, showed approximately 2-fold increase in their cytotoxicity against HeLa tumor cells in the presence of the induced microsomal generating system suggesting that their cytooxicity depends on the drug metabolic transformation into their active metabolites (intermediates) via MFO-system, and that structural unit of this chain is essential for abovementioned activation. Presently found remarkable cytotoxic selectivity of acyclovir analogues against KB and HeLa tumor cells together with previously reported in the literature specific cytotoxic activity of acyclovir against murine leukemia L1210 cells seem to be encouraging for further investigation of this class of compounds in other tumor systems.

510/2523

Tytuł oryginału: Synthesis, antibacterial, antifungal and genotoxic activity of bis-1,3,4-oxadiazole derivatives.
Autorzy: Maslat Ahmed O., Abussaud Mahmud, Tashtoush Hasan, AL-Talib Mahmoud
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.55-59, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In the present investigation, four 1,3,4-bis-oxadiazole derivatives were synthesized as potential antimicrobial agents. The compounds are: 5,5'-dimercapto-bis-[1,3,4-oxadiazol-2-yl]propane (2a), 5,5'-dimercapto-bis-[1,3,4-oxadiazol-2-yl]butane (2b), 5,5'-dimercapto-bis-[1,3,4-oxadiazol-2-yl]octane (2c) and 5,5'-dibenzylthio-bis-[1,3,4-oxadiazol-2-yl]butane (3). The above newly synthesized compounds were investigated for their antibacterial, antifungal and mutagenic activities. The results of the biological activities revealed that the compounds 2a-c exhibited both antibacterial and antifungal activities against S. aureuss and B. subtilis. Compound 2a also showed activity against P. aeureoginosa. All the above compounds and compound 3 exhibited activity against C. albicans. Genotoxic studies showed that compound 2a had a weak base pair substitution mutagenicity but none of them exhibited a frameshift mutagenic action using Ames test.

511/2523

Tytuł oryginału: Prolonged treatment with glucocorticoid dexamethasone suppresses melatonin production by the chick pineal gland and retina.
Autorzy: Zawilska Jolanta B., Sadowska Magdalena
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.61-66, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ptaki

Streszczenie angielskie: The chick pineal gland and retina synthesize melatonin in a circadian rhythm with high levels during the night. The rhythmic changes in the hormone production result predominantly from the fluctuation in the activity of serotonin N-acetyltransferase (AA-NAT), a penultimate and key regulatory enzyme in melatonin biosynthesis. The aim of this study was to analyze the effects of an acute and prolonged in vivo treatment with a glucocorticoid dexamethasone (4 mg/kg, ip) on the nocturnal increase in AA-NAT activity in chick pineal gland and retina. In acute experiments, dexamethasone (single dose)-injected chicks were killed after 2 h, while in prolonged experiments the glucocorticoid was given once daily for 7 days and the animals were killed 26-32 h after the last injection. Acute administration of dexamethasone did not affect AA-NAT activity in the chick pineal gland and retina. In the pineal glands and retinas of chicks that were treated with dexamethasone for one week and then killed at the end of the light phase of the 12:12 h light-dark cycle, AA-NAT activity was significantly higher than the enzyme activity found in tissues isolated from the vehicle-treated (control) animals. In addition to that, the nocturnal increase in pineal and, to a lower extent, retinal AA-NAT activity was significantly lower in dexamethasonetreated birds when compared with the respective control groups. It is suggested that prolonged treatment of animals with dexamethasone reduces the amplitude of the rhythmic melatonin production, a phenomenon which may affect chronobiological processes being under control of this hormone.

512/2523

Tytuł oryginału: In vitro effects of some anesthetic drugs on enzymatic activity of human red blood cell glucose 6-phosphate dehydrogenase.
Autorzy: Altikat Sayit, Ciftci Mehmet, Bykokuroglu Mehmet E.
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.67-71, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The study investigated in vitro effects of halothane, isoflurane, ketamine, sevoflurane, prilocaine, diazepam, and midazolam on enzymatic activity of human red blood cell glucose-6-phosphate dehydrogenase (G6PD; E.C. 1.1.1.49). G6PD was purified from human red blood cells by 2',5'-ADP-sepharose 4B affinity gel. Enzymatic activity was spectrophotometrically measured at 340 nm according to the method of Beutler. I50 values were determined from drug activity 9p.c.) - drugconcentration curves. I50 values were as follows: 0.72 mM for isoflurane, 1.82 mM for sevoflurane, 0.38 mM for diazepam, and 0.0019 mM for midazolam. But halothane, ketamine and prilocaine had no inhibitory effect on the G6PD activity in in vitro.

513/2523

Tytuł oryginału: Inhibition of arachidonic acid cascade attenuates the induction of c-Fos proteins by DOI, 5-HT2A/2C receptor agonist, in the rat cortex.
Autorzy: Maćkowiak Marzena, Czyrak Anna, Wędzony Krzysztof
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.73-76, il., bibliogr. 9 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Previous immunohistochemical studies have shown that c-Fos proteins induced by DOI, a 5-HT2A/2C agonist, are present in the population of cortical neurons, which are devoid of 5-HT2A receptors. A mechanism of the induction of c-Fos proteins expression by DOI is still unclear. However, the involvement of the 5-HT2A and AMPA, but not 5-HT2C receptors in this process has been reported. In the present study, we investigated whether arachidonic acid, a retrograde messenger, is involved in the above mechanism of c-Fos induction. Phospholipase A2 pathway, which leads to the subsequent generation of arachidonic acid and its metabolites, is known to be coupled to 5-HT2A receptor activation. The inhibition of arachidonic acid cascade both at the level of phospholipase A2 (by dexamethasone, 1.5 mg/kg0 or at the level of cyclooxygenases that catalyze arachidonic acid biotransformation (by indomethacin, 3 mg/kg), decreased the number of c-Fos immunopositive cells after induction by DOI (8 mg/kg). Our results suggest that arachidonic acid cascade may be involved in the induction of c-Fos proteins by DOI in the rat parietal cortex.

514/2523

Tytuł oryginału: Sixth Conference on Progress in Etiopathogenesis of Seizures.
Źródło: Pol. J. Pharmacol. 2002: 54 (1) s.77-84 - 6 Konferencja nt. etipatogenezy drgawek Lublin 16.11. 2001
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

streszczenie

Wskaźnik treści:

515/2523

Tytuł oryginału: Emotional changes related to age in rats - a behavioral analysis.
Autorzy: Boguszewski Paweł, Zagrodzka Jolanta
Źródło: Behav. Brain Res. 2002: 133 (2) s.323-332, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 312,662

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The present study investigated age-related differences in the emotional behavior of rats using factor analysis to identify motivational factors influencing spontaneous behavior in open field with illuminated center (OF), plus maze (EPM) and social interactions test. Animals of the same strain, bred under the same conditions, formed two experimental groups: yound adults (YA, N - 20) tested at the age of 4 months and old rats (OA N = 16) tested at the age of 24 months. The comuter video based tracking system Etho Vision was used for automated acquisition and analysis of data. The reslults of each test were analyzed separately for YA and OA by factor analysis. Two main independent factors emerged from the analysis of OF measures - factor 1, which appeared to reflect motor activity, and factor 2, reflecting anxiety. The measures best reflecting motor activity (distance moved in the peripheral zone) and anxiety (time spent in central zone) decreased significantly with age. Factor analysis for EPM measures revealed, in both groups, three independent factors. In YA, factor 1 reflected motor activity, factor 2 - anxiety, in OA measures of anxiety loaded on factor 1, measures of activity on factor 2. Factor 3 in both groups appeared to represent a decision making process. The number of entries to the closed arms declined significantly in OA, showing an age related decrease of motor activity. Also, the ratio of open arms entries in relation to the total number of entries decreased in OA, indicating a higher anxiety level...

516/2523

Tytuł oryginału: Obesity-related hypertension: relevance of vascular responses to mental stress.
Autorzy: Narkiewicz Krzysztof
Źródło: J. Hypertens. 2002: 20 (7) s.1277-1278, bibliogr. 21 poz.
Sygnatura GBL: 313,132

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

517/2523

Tytuł oryginału: Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea.
Autorzy: Paciorek Jarosław
Źródło: J. Med. Microbiol. 2002: 51 (7) s.548-556, il., tab., bibliogr. 58 poz.
Sygnatura GBL: 305,350

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Four hundred and twetny-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enteroheamolysin, the genes encoding intimin (eae), bundle-forming pili (bfp), Shiga toxins I and II (stxI, stxII) and cytotoxic necrotising factor types 1 and 2 (cnf1, cnf2) was investigated by PCR. Adhesion to HEp-2 cell monolayers was also tested and selected strains were investigated for the presence of P-fimbriae and haemolytic activity. Typical enteropathogenic E. coli isolates (typical EPEC, strains possessing eae and bfp, but not stx) were not found. The particular classes of E. coli among 427 isolates from ill children were: atypical EPEC (eae**+ bfp, stx**-), 21.3 p.c.; Shiga toxin-producing E. coli (STEC), 0.7 p.c.; diffsely adherent E. coli (DAEC), 4 p.c.; enteroaggreagative E. coli (EAEC), 16.9 p.c.; necrotoxic E. coli type 1 (NTEC1), 0.2 p.c.; and cell-detaching E. coli (CDEC), 29 p.c. With the exception of STEC, all the above classes of E. coli were found among the isolated from healthy children which comprised: atypical EPEC 8.0 p.c., DAEC 6.7 p.c.; EAEC 17.3 p.c., NTEC1 14.0 p.c. and CDEC 40.0 p.c. Cell detachment (CD) was significantly associated with 3-h haemolytic activity. There was also strong correlation between haemolytic activity (Hly) and the presence of p-fimbriae. No correlation was found between the presence of te cnf1 gene and CD, Hly or P-fimbriae.

518/2523

Tytuł oryginału: Cytokine serum levels in soft tissue sarcoma patients: correlations with clinico-pathological features and prognosis.
Autorzy: Rutkowski Piotr, Kamińska Janina, Kowalska Maria, Ruka Włodzimierz, Steffen Jan
Źródło: Int. J. Cancer 2002: 100 (4) s.463-471, il., tab., bibliogr. 85 poz.
Sygnatura GBL: 305,029

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: We investigated the correlations between serum levels of selected proinflammatory, hematopoietic and angiogenic cytokines and soluble cytokine receptors with the clnicopathological features and prognosis in soft tissue sarcom patients. Serum levels of 9 cytokines (TNFŕ, IL-Ira, IL-6, IL-8, IL-10, M-CSF, G-CSF, VEGF, bFGF) and 4 free cytokine receptors (sIL-2Rŕ, sIL-6R, TNFRI TNFRII) were measured by means of an enzyme-linked immunoadsorbent assay kit in 156 soft tissue sarcoma patients before the treatment and in 50 healthy controls. Serum levels of 10 cytokines and cytokine receptors were also assayed during patients' follow-up after the treatment. Significantly elevated pretreatment serum levels of 11/13 cytokines and cytokine receptors were found in sarcoma patients, as compared to healthy controls. In 40.4 p.c. of patients 6 or more cytokines and cytokine receptors (most frequently: TNF RI, IL-6, IL-8) were elevated in parallel. Serum levels of IL-6, sIL-2R, VEGF, M-CSF and TNF RI correlated significantly with tumor size and serum levels of IL-8 and IL-6 were significantly higher in patients with Grade 2/3 vs. Grade I tumors. We did not observe any significant differences in cytokine serum levels between patients with primary and recurrent tumors and patients with and without distant metastases. Using univariate analysis, overall survival (OS) in all patients was affected by tumor size ( 5 cm vs. 5-10 cm vs. 10 cm), tumor grade (GI vs. G2/3), presence of metastases, pretreatment serum levels of 8 cytokines...

519/2523

Tytuł oryginału: Professor Bogusław Żernicki (1931 - 2002).
Autorzy: Wróbel Andrzej, Zagrodzka Jolanta, Doty Robert, Zieliński Kazimierz, Kozak Włodzimirz M., Mishkin Mortimer, Buser Pierre, Buser Arlette, Harutiunian-Kozak Bella, Turlejski Krzysztof, Morrison Adrian R., Brennan James F., Sobótka Stanisław, Yinon Uri, Gottesmann Claude, Villablanca Jaime R., Wilson W. Jeffrey
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.i-ix, il.
Sygnatura GBL: 302,090

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Żernicki

Bogusław 1931-2002

520/2523

Tytuł oryginału: Involvement of MMPs in delayed neuronal death after global ischemia.
Autorzy: Zalewska Teresa, Ziemka-Nałęcz Małgorzata, Sarnowska Anna, Domańska-Janik Krystyna
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.53-61, il., bibliogr. s. 59-61
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Spatial and temporal relations between metalloproteinase (MMP-2 and MMP-9) activation and laminin degradation in garbil hippocampus after transient cerebral ischemia has been studied. Activity of MMPs was determined by gelatin zymography in homogenates from dorsal (DP, an equivalent of CA1 sector) and abdominal (AbP, containing CA2-4 and gyrus dentatus) parts of hippocampus. A significant activation of both investigated metalloproteinases was found at 72 h of recovery. Whereas MMP-2 up-regulation did not show any spatial preferences, the increase of MMP-9 activity was observed exclusively in DP. Activation of MMP-9 at this time point correlated spatially with degradation of laminin - protein of extracellular matrix. These results show that MMP pathway may function as a component of delayed neuronal death cascade in the apoptogenic CA1 sector after transient global ischemia.

521/2523

Tytuł oryginału: Fear behavior and regional brain monoamines distribution after R(+)-8-OHDPAT and R(+)-UH-301 injections into the dorsal raphe nucleus in rats.
Autorzy: Koprowska Milena, Krotewicz Maria, Romaniuk Andrzej, Strzelczuk Magdalena, Wieczorek Marek
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.63-74, il., tab., bibliogr. s. 73-74
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The effects of 8-OHDPAT and UH-301 injection into the dorsal raphe nucleus (DRN) on fear behavior of the light-dark transitions test and regional brain monoamines (NA, DA, 5-HT) and their metabolites (MHPG, DOPAC, 5-HIAA) in the hypothalamus (HPT), midbrain central gray matter (MID), amygdala (AMY), hippocampus (HIP) and pons (PO) were examined. An injection of 8-OHDPAT (300 ng) as well UH-301 (300 ng) into the DRN evoked an increase in the number of head dipping from dark to the iluminated compartment of chamber, an increase of time of motionless in the dark compartment and decrease of time of locomotion activity in the illuminated compartment. HPLC analysis showed reduction of 5-HIAA/5-HT ratio in the HPT, HIP and PO, increase of MHPG/NA ratio in the HIP and PO, and increase of DA content in the HPT, AMY and HIP after 8-OHDPAT injection. But injection of UH-301 reduced 5-HT in the MID and increased in the AMY, reduced 5-HIAA content in the HIP and increased in the MID and decreased MHPG/NA ratio in the PO. These results indicate that both 5-HT1A receptor agonists, acting on the 5HT1A autoreceptors caused the anxiolytic effects, reduced fear behavior on the rat connected with infringement of dynamic balance between the serotonergic and catecholaminergics systems.

522/2523

Tytuł oryginału: Behavioural responsiveness to amphetamine or scopolamine following repeated exposure to chlorphenvinphos in rats.
Autorzy: Gralewicz Sławomir, Lutz Piotr, Tomas Tadeusz
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.75-83, il., tab., bibliogr. [24] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: A number of reports indicate that exposure to organophosphates (OPs), inhibitors of acetylcholinesterase (AChE), may result in long-lasting neurobehavioural alterations suggestive of an increased cholinergic tone. It is known that rats with cholinergic hyperreactivity are behaviourally hyposensitive to cholinergic antagonists and dopaminergic agonists. The purpose of the present study was to find out whether a similar trait would develop in rats exposed to chlorphenivinphows (CVP), an OP pesticide, in the past. The rats wre given ten daily i.p. injections of CVP at doses of 0.5 mg/kg (group P-0.5) or 1.0 mg/kg (group P-1.0). The locomotion stimulating effect of i.p. injection of 1.0 mg/kg amphetamine (AMPH), or 0.6 mg/kg scopolamine (SCOP), was assessed on postexposure day 21 (group P-0.5) or 42 (group P-1.0), i.e. after a time suffcient for AChE recovery. The assessment revealed that in gorup P-1.0 the behavioural response to AMPH and SCOP was significantly depressed. In rats of the P-0.5 group, however, the behavioural response to each of the frugs was increased. The results suggest that, depending on the exposure level, contrasting alterations in some neurotransmitter systems may be induced by repeated exposure to CVP.

523/2523

Tytuł oryginału: Correlation between long latency evoked potentials from amygdala and evoked cardiac response to fear conditioned stimulus in rats.
Autorzy: Kuniecki Michał, Coenen Anton M. L., Kaiser Jan
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.85-92, il., bibliogr. s. 90-92
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: We investigated relation between activity of central nucleus of amygdala (CE) and phasic heart rate deceleration during differential fear conditioning. We found that P2 component of long-lasting event potential (EP) to CD+ but not to CS- correlated strongly with HR deceleration in the 1 st second after stimulus onset. The obtained results are discussed in the light of LeDoux's and Kapp's findings showing crucial role of amygdala in processing of emotionally relevant stimulation and it's involvement in initiating autonomic responses.

524/2523

Tytuł oryginału: Behavioral sensitization to amphetamine induced by a single i.p. dose of oxotremorine in the rat.
Autorzy: Gralewicz Sławomir
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.93-98, il., bibliogr. s. 97-98
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Earlier experiments have revealed that rats treated with a single dose of chlorphenvinphos (CVP), an irreversible acetylchlinesterase inhibitor, are hyposensitive to amphetamine (AMPH) given three weeks after CVP. Exposure to CVP results in an excess of acetylcholine with subsequent overactivation of the nicotinic as well as muscarinic cholinergic receptors. The purpose of the present experiment was to find out whether a selective activation of muscarinic receptors could induce behavioral hyposensitivity to AMPH. To attain this purpose, male rats were pretreated once with 0.00, 0.135, 0.27 or 0.55 mg/kg of oxotremorine, a muscarinic agonist, and challenged 15 days later with 1.0 mg/kg dose of AMPH. The pre- and postinjection open-field behavior of the rats was tested with the use of a computerized set of activity meters. The testing revealed that in oxotremorine pretreated animals the behvioral response, to AMPH, i.e. increase in the ambulatory activity, was not diminished but, to the contrary, it was augmented. This effect was dose-dependent, being most pronounced in rats given the 0.55 mg/kg of oxotremorine. The possible cause of the difference between the effect of CVP and oxotremorine is discussed.

525/2523

Tytuł oryginału: Affective percept and voluntary action: a hypothesis.
Autorzy: Żernicki Bogusław
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.99-110, il., tab., bibliogr. s. 108-110
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: I present a hypothesis concerning the neuronal, mental and behavioral effects of all kinds of affective (emotional) stimuli, i.e., of unpleasant and pleasant stimuli. I use the term stimulus in its broad sense. Affective stimuli evoke two associated percepts: "cognitive" and "affective". A food in the mouth, for example, evokes the gustatory percept and the percept of pleasure. However, affective percepts are unstable parts of cognitive-affective compounds. Five types of affective percepts are pain, fear, pleasure, "desire" and appetite in the broad sense of these words. Desire is evoked by inadequate pleasant stimuli. Affective percepts are "lower" or "higher". The latter are not directly associated with bodily needs. Esthetic and social percepts are higher, for example. Although pain and pleasure are essentially innate, they can be modified by sensory experience. Alimentary and esthetic preferences and social values are modifiable, for example. The neurons of pain and fear and of the unpleasant components of desire and appetite motivate four types of voluntary actions. These are, respectively, escape, avoidance, "optimization" and approach actions. All these actions eliminate the motivating displeasure. In addition, avoidance actions protect from the signaled pain, optimization actions increase the existing pleasure and approach actions provide the signaled pleasure. Thus, voluntary actions associated with different percepts occur according to one universal principle. Voluntary actions are "internal" and behavioral. During internal actions a goal and then an action plan are decided. These actions often provide the images of the goal stimuli and of particular movements.

526/2523

Tytuł oryginału: Drug addiction as drive satisfaction ("antidrive") dysfunction.
Autorzy: Kostowski Wojciech
Źródło: Acta Neurobiol. Exp. 2002: 62 (2) s.111-117, il., bibliogr. [31] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Drug addiction is a complex brain disorder, characterized by the loss of control over drug seeking and drug taking behavior, and by the risk of relapse, even after a prolonged period of abstinence. This disorder may have its source in a disturbed balance of drive-related behaviors, which control appetitive reactions aimed at seeking contact with an addictive substance. The act of consumption becomes more and more attractive, and the behavior takes on compulsive character. We suppose that drug addiction may involve a change in the mechanism of satisfaction of drives and states of satiation as well. To understand how the motivational processes are changed with the development of dependence, one must consider the mechanism of drive satisfaction and satiation states that occur in relation to the consumatory reflex. When a given drive is satisfied a state of fulfilament occurs. This state may be a result of a so-called "antidrive" mechanism (Konorski 1967). While a drive activity is characterized by general activation and tension, the drive satisfaction state ("antidrive") is characterized by relaxation and releif. When a particular drive is satisfied, the operation of other drives become possible. Therefore, we postulate that dysfunction of drive satisfaction leads to the sustained activation related to the current drug-related drive, which blocks the operation of other drives. In effect, uncontrolled compulsive appetitive behavior is released, and the operation of other drives is restrained, thus forcing the organism to focus on drug-related drive. The reason for an "antidrive" dysfunction may be related to adaptive changes which develop during a contact with an addictive substance.

527/2523

Tytuł oryginału: Environmental thermal stress.
Autorzy: Keim Samuel M., Guisto John A., Sullivan, jr John B.
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.1-15, il., tab., bibligor. 59 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

ochrona środowiska

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Thermal stress from cold and heat can affect health and productivity in a wide range of environmental and workload conditions. Health risks typically occur in the outer zones of heat and cold stress, but are also related to workload. Environmental factors related to thermal stress are reviewed. Individuals undergo thermoregulatory physiologic changes to adapt and these changes are reviewed. Head and cold related illnesses are reviewed as well as their appropriate therapy. Published standards, thresholds and recommendations regarding work practices, personal protection and types of thermal loads are reviewed.

528/2523

Tytuł oryginału: Becterial and fungal aerosols in indoor environment in Central and Eastern European countries.
Autorzy: Górny Rafał L., Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.17-23, tab., bibliogr. 34 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Studies of indoor bioaerosols conducted in Central and Eastern European countries, as a result of the scarcity of funding, mostly do not attain the level presented by similar studies in Northern America and Western Europe. For socio-economic reasons, most of the intense studies on indoor bioaerosols in Central and Eastern European countries were carried out in industrial facilities and have contributed significantly to occupational health science. In contrast, until recently, insufficient of studies have been conducted on bioaerosols of residental and communal premises (dwellings, offices, schools, etc.) and no network for monitoring the microbiological quality of air in such premises exists. In Poland, in the mid-1990s complex bioaerosol investigations were carried out by the Bioaerosol Group at the Institute of Occupational Medicine and Environmental Health in Sosnowiec. The concentrations of airborne bacteria and fungi in dwellings without mold problems were between 88 - 4297 cfu/m**3 and 0 - 1997 cfu/m**3, while in moldy homes they were 178 - 4751 cfu/m**3 and 49 - 16968 cfu/m**3, respectively. As many as 167 microbial species were isolated from the air of examined dwellings. Most frequently occurred Gram-positive cocci (Micrococcus/Kocuria spp., Staphylococcus spp.), endospore-forming bacilli (Bacillus spp.), Gram-negative bacteria (Pseudomonadaceae, Aeromonas spp.), filamentous fungi (Penicillium spp., Aspregillus spp.), and yeasts. Notable studies of indoor bioaerosols have also been performed in the other Central and Eastern European countries: Lithuania, Latvia, Estonia,...

529/2523

Tytuł oryginału: Coexistence DNA of Borrelia burgdorferi sensu lato and Babesia microti in Ixodes ricinus ticks from north-western Poland.
Autorzy: Skotarczak Bogumiła, Wodecka Beata, Cichocka Agnieszka
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.25-28, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The tick Ixodes ricinus may carry microorganisms which cause serious human and animal diseases, i.a., the Lyme diseases (borereliosis), caused by the spirochaete Borrelia burgdorferi sensu lato and babesiosis, induced by the protozoan Babesa microti. Both microbe species may co-occur in the same and other species of the genus tick and produce a mixed infection in humans and animals. The major objective of the study was to identify DNA of B. burgdorfere and B. microti in the I. ricinus ticks collected in spring and autumn 1999 from 6 sites in north-western Poland. The microbial DNA was identified with polymerase chain reaction (PCR). The marker used to detect the B. burgdorferi s.l. DNA was a fragment of the fla gene encoding the protein flagellin, while the B. microti DNA was detected with a fragment of the gene encoding 16S rRNA. A total of 550, 1,160, and 385 tick adults, nymphs, and larvae, respectively, were examined. Among the 155 (7.4 p.c.) B. burgdorferi-infected ticks and the 130 (6.2 p.c.) infected with B. micron, mixed infection was detected in 0.6 p.c. of individuals. The prevalence of coinfection differed between the tick developmental stages. Coinfection was most prevalent (3.1 p.c.) in females, males and nymphs being less affected (0.4 and 0.2 p.c., respectively). No coinfection was revealed in the tick larvae. The study described was the first of its kind to be conducted in the former District of Szczecin. For the phenomenon of microbial co-occurence and related mixed infections to be properly evaluated, the researach will be continued.

530/2523

Tytuł oryginału: The use of the radioisotope method in studies of pesticide penetration into the eyeball.
Autorzy: Gerkowicz Marek, Wróblewska Ewa, Turski Waldemar
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.29-31, bibliogr. 10 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: The studies concerning the effects of pesticides on the human body focused mainly on their local action or chronic poisoning of the organism. In this study we examine the pesticide penetration into the eyeball resulting from direct contact with the eye. We used an isotope-labelled carbamate pesticide - carbaryl. The determinations of the amount and concentration of this substance in the cornea, aqueous humour, vitreous humour and retina were performed using the method measuring á radiation emitted by radioactive carbon. The results revealed measurable concentrations of labelled carbamate in the cornea and aqueous humour 10 and 30 min after application. The levels of this pesticide in the vitreous homour and retina were very low and difficult to analyse statistically. The described method appears to be useful in determining the range and rate of eye penetration by environmental toxings having direct contact with the eyeball.

531/2523

Tytuł oryginału: House dust mites and their allergens in Danish mattresses - results from a population-based study.
Autorzy: Sidenius Kirsten E., Hallas Thorkil E., Poulsen Lars K., Mosbech Holger
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.33-39, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The purpose of this study was to identify the level of house dust mites (HDMs) and their allergens in matresses, not selected on their owners atopic status, and to find associated factors. Dust was collected from 68 matresses. The recruitment was population-based and conducted during the screening phase of a HDM intervention study. The visited persons declared to have had a "cold" bedroom the previous winter. HDMs were counted and dust was analysed by ELISA for Der 1 (= Der f 1 + Der p 1 + Der m 1). Multiple regression analysis was carried out to find housing conditions associated with high MDM levels. Type of housing, mattress age and self-assessed winter bedroom-temperature explained 14 p.c. of Der 1. Median concentrations were 3.77 ćg Der 1/g and 1 HDM/0.1 g dust. Both immunochemically and microscopically Dermatophagoides farinae was dominant; D. pteronyssinus less frequent but important; and D. microscopisally Dermatophagoides farinae was dominant; D. pteronyssinus less frequent but important; and D. microceras insignificant. In 62 p.c. of these suburban homes the mattress dust exceeded 2 ćg Der 1/g, and measurement of both Der f 1 and Der p 1 was necessary and sufficient to evaluate HDM allergen exposure. The association with a high HDM level was highest and most consistent for one-family houses.

532/2523

Tytuł oryginału: Air contaminants in different European farming environments.
Autorzy: Radon Katja, Danuser Brigitta, Inversen Martin, Monso Eduard, Weber Christoph, Hartung Jrg, Donham Kelley J., Palmgren Urban, Nowak Dennis
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.41-48, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Farmers are known to be at high risk from the development of occupational airway disease. The first stage of the European farmers' study has shown that pig farmers in Denmark and Germany, poultry farmers in Switzerlad and greenhouse workers in Spain were at highest risk for work-related respiratory symptoms. Therefore, the aim of this study was to determine exposure levels at relevant farm workplaces. Dust and endotoxin levels as well as microbiological concentrations were determined in 213 crop and animal farming environments by personal sampling. The highest total dust concentrations were found in poultry houses in Switzerland with median concentrations of 7.01 mg/m**3. The median airborne endotoxin concentrations in total dust ranged between 0.36 ng/m**3 in Spanish greenhouses and 257.58 ng/m**3 in poultry houses in Switzerland. Likewise, the highest median concentrations of total (2.0 x 10**7 cells/m3) and active fungi (4.4 x 10**5 cfu/m**3) have been found in Swiss poultry houses. The predominant fungus taxa discovered in poultry houses were Eurotium spp. and thermophilic fungi. Cladosporium and Botrytis were mainly detecated in greenhouses. The exposure level found in this study might put the farmers at risk from respiratory diseases.

533/2523

Tytuł oryginału: Second inter-laboratory study comparing endotoxin assay results from cotton dust.
Autorzy: Chun David T. W., Chew Victor, Bartlett Karen, Gordon Terry, Jacobs Robert R., Larsson Britt-Marie, Lewis Daniel M., Liesivuori Jyrki, Michel Olivier, Rylander Rangar, Thorne Peter S., White Eugene M., Gunn Varina C., Wrtz Helle
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.49-53, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Previously, a large two-part inter-laboratory round robin endotoxin assay study was completed. This first study showed that when cotton dust samples, which are practically identical, are assayed for endotoxin that the intra-laboratory results had a very small variation while intra-laboratory results of the sample had a very high variation. In the first part of the study, each laboratory results of the sample had a very high variation. In the first part of the study, each laboratory followed its own in-house assay protocol; but in the second part of the study, when the extraction protocol was standardized, the inter-laboratory results showed a lower variation, which suggested that with further standardization, further reduction of differences between laboratories might be achieved in order that results between laboratories would become more comparable. The results stimulated interest in extending the study to include cotton dust with two levels of endotoxin, standardization of the extraction proteocol, and using the same assay kit from the same production lot. The results of this second round robin endotoxin assay study indicate that differences between laboratories are still high, but most of the laboratories could discern the cotton dusts with the different levels of endotoxin.

534/2523

Tytuł oryginału: Detection of Borrelia burgdorferi sensu lato in mosquitoes (Culicidae) in recreational areas of the city of Szczecin.
Autorzy: Kosik-Bogacka Danuta, Bukowska Katarzyna, Kuźna-Grygiel Wanda
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.55-57, tab., bibliogr. 29 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The mosquitoes were caught within the period of their highest activity, i.e. June-August 2001, in recreational areas of the city of Szczecin. Spirochetes, Borrelia burgdorferi sensu lato were detected in mosquitoes with the aid of the method of indirect immunofluorescence (IFA) using rabbit anti-Borrelia burgdorfere antibodies and goat anti-rabbit IgG marked with fluorescein isothiocyanate (FITC). A total of 639 mosquito females representing genera Ades (99.1 p.c.) and Culex (0.9 p.c.) were collected. The mean value of the infection rate of mosquitoes from the area studied was 1.25 p.c. The highest infection rate was recorded in June (3.2 p.c.), while the lowest - in July (0.6 p.c.). All mosquitoes infected with Borrelia burgdorferi spirochetes belonged to the gnus Ades. The results of the present study confirm a potential role of those arthropods in epidemiology of Lyme borreliosis.

535/2523

Tytuł oryginału: Indoor fungi and their ciliostatic metabolites.
Autorzy: Pieckova Elena, Kunova Zuzana
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.59-63, tab., bibliogr. 31 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zarodki

Streszczenie angielskie: According to epidemiological studies, it is possible that some secondary metabolites of indoor airborne fungi could be responsible for health troubles which occupants sufer from. In our previous experiments, a model with tracheal rings of 1-day-old chicks in vitro was shown to be a very suitable method to study the ciliostatic chloroform-extractable endo- end/or exometabolites of filamentous fungi. In this study we isolated the filamentous fungi from walls of "moudy" dwellings and schools (cultivation on dichloran 18 p.c. glycaerol agar at 25 and 37řC for 10 d) in Slovakia. We studied the ciliostatic effect of the chloroform-extractable endo- and exometabolites of 96 representative isolates (Stationary cultivation on the liquid medium with 2 p.c. of yeast extract and 10 p.c. of sucrose at 25řC for 10 days) on the cilia movement in tracheal organ cultures of 1-day-old chickens in vitro after 24, 48 and 72 hrs (incubation in the minimal essential medium according to Eagle with Earl's salts and 20 ćg of extract of metabolites dissolved in dimethylsulfoxide per 1 mL). Strains of Penicillium Link: Fr. sp., Aspregillus versicolor (Vuill.). Tiraboschi, A. flavus Link, Cladosporium sphaerospermum Penzig adn C. cladosporioides (Fres.) de Vries were isolated most frequently. Two A. flavus isolates were able to produce aflatoxins B1, B2, G1, G2 in vitro after cultivation on the liquid medium with 20 p.c. sucrose and 2 p.c. yeast extract. This is the first isolation of aflatoxigenic A. flavus strains from dwellings in Slovakia. All frequently isolated strainsproduced secondary metabolites...

536/2523

Tytuł oryginału: Betula and Populus pollen counts and meteorological conditions in Szczecin, Poland.
Autorzy: Puc Małgorzata, Wolski Tomasz
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.65-69, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,158

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The aim of the study was to analyse a relationship between the meteorological conditions and the pollen count of selected allergenic taxa (Betula spp., Populus spp.) in the air of Szczecin city (north western Poland) in 2001. Apart from the individual rhythm of plant pollination, weather conditions are considered the most important factors determining the dispersion and content of pollen in the air. The meteorological parameters analysed were the minimum, maximum and average values of air temperature, relative humidity, solar radiation and wind speed. The beginning and end of a season were established by the 98 p.c. method. The concentration of birch pollen in the air in 2001 was very high, the pollen season started in the third decade of April and lasted till the 10 May. The highest airborne concentration of 3.712 grains in 1 m**3 per 24 h was noted at the beginning of May on a sunny day with strong wind, and air temperature above 20řC. The concentration of poplar pollen in 2001 was low. The pollen season started from the beginning of April and lasted till the beginning of May. The maximum concentration of airborne poplar pollen of 222 grains in 1 m**3 per 24 h was observed in the third decade of April and was preceded by several days with low temperature (1 - 2řC), rain, snowfalls and strong wind. A positive and statistically significant correlation was found between the air temperature and the birch pollen concentration, while a similar but negative correlation was found for poplar pollen.

537/2523

Tytuł oryginału: Qualitative and quantitative analysis of agricultural dust in working environment.
Autorzy: Mołocznik Anna
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.71-78, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The presented quantitative and qualitative analysis of dust in agricultural working environment is a continuation of the process of recognizing the exposure to dust among private farmers. The study covers the following: determination of respirable fraction of dust in the respiratory zone (on the background of total dust) whileperforming individual farming activities which constitute an annual work cycle, organic and mineral components of settled dust for basic groups of farming activities, and the main mineral pathogenic component - free silica in airborne and setled dust. The study was conducted on 5 farms specialising in: cultivation of cereals, root plants, vegetables, dairy cattle and swine breeding and mixed production. The analysis of settled dust covered 17 types of dust accompanying field work and farm/indoor activities. Studies of airborne dust were conducted on farmers while performing 40 main work activities which contributed to the annual work cycle. Results of the study confirmed the following: agricultural work activities are accompanied by a high level of dustiness and showed the presence of a respirable fraction in airborne dust of up to 25 p.c., a higher level of pathogenic free silica SiO2 in setled dust samples in the working environment of a farmer, compared to dust in respiratory zone, a comparable level of SiO2 in total and respirable airborne dust, and a high level of organic component in settled dust at work activities with plant material. These results indicated that the evaluation of farmers' exposure to dust should be based on the examination of samples taken in the respiratory zone while performing individual work activities.

538/2523

Tytuł oryginału: Selected socio-economic features and the prevalence of peptic ulcer among Polish rural population.
Autorzy: Schabowski Janusz
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.79-84, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: The aim of the study was to determine the realtionship between the prevalence of peptic ulcer and the occurrence of selected socio-economic features among Polish rural population. The study was conducted based on the all-Polish representative study of the state of health of rural population, and covered a group of 6,512 rural inhabitants age 20 - 64 - 3,107 males and 3,405 females selected by twostage stratified sampling. Peptic ulcer was diagnosed in 348 people in the study (5.3 p.c.): 250 males (8.0 p.c.) and 98 females (2.9 p.c.). Duodenal ulcer occurred in 3.2 p.c. of people examined, followed by gastric ulcer - .2 p.c., duodenal and gastric ulcer - 0.2 p.c., and 0,9 p.c. of patients underwent surgical procedures due to peptic ulcer. Peptic ulcer occurred more frequently among people with a lower education level (lack of education - 7.8 p.c., elementary school education - 5.8 p.c.), compared to those with higher education categories (elementary vocational - 4.9 p.c., secondary school and college - 3.7 p.c.). The disease was more often diagnosed among respondents who described their material standard as poor (7.7 p.c.), compared to those who described this standard as good (4.0 p.c.). Among people who considered their material standard as poor, gastric ulcer was noted more frequently than duodenal ulcer. A correlation was observed between the prevalence of peptic ulcer and such socio-economic features of Polish rural population as the level of education and material standard.

539/2523

Tytuł oryginału: Exposure to airborne microorganisms in furniture factories.
Autorzy: Krysińska-Traczyk Ewa, Skórska Czesława, Cholewa Grażyna, Sitkowska Jolanta, Milanowski Janusz, Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.85-90, il., tab., bibliogr. 57 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Microbiological air sampling was performed in 2 furniture factories located in eastern Poland. In one factory furniture were made from fibreboards and chipboards while in the other from beech wood. It was found that the concentration of total microorganisms (bacteria + fungi) in the air of the facility using beech wood for furniture production (mean 10.7 x 10**3 cfu/m3, range 3.3 - 27.5 x 10**3 cfu/m**3) was significantly higher (p 0.01) compared to microbial concentration in the facility using fibre- and chipboards (mean 3.6 x 10**3 cfu/m3, range 1.9 - 6.2 x 10**3 cfu/m**3). On average, the commonest microorganisms in the air of the furniture factories were corynebacteria (Corynebacterium spp., Arthrobacter spp., Brevibacterium spp.) which formed 18.1 - 50.0 p.c. of the total airborne microflora, and fungi (mostly Aspergillus spp., Penicillium spp. and yeasts) which formed 6.2 - 54.4 p.c. of the total count. The values of the respirable fraction of airborne microflora in the furniture factories varied within fairly wide limits and were between 15.0 - 62.4 p.c. Altogether, 28 species or genera of bacteria and 12 species or genera of fungi were identified in the air of examined factories, of which respectively 8 and 7 species or genera were reported as having allegenic and/or immunotoxic properties. In conclusion, the workers of furniture factories are exposed to relatively low concentrations of airborne microorganisms which do not exceed the suggested occupational exposure limits. Nevertheless, the presence of allergenic and/or immunotoxic microbial species in the air of factories poses a potential risk of respiratory disease, in particular in sensitive workers.

540/2523

Tytuł oryginału: Response of furniture factory workers to work-related airborne allergens.
Autorzy: Skórska Czesława, Krysińska-Traczyk Ewa, Milanowski Janusz, Cholewa Grażyna, Sitkowska Jolanta, Góra Anna, Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.91-97, tab., bibliogr. 46 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of this work was to determine the reactivity of furniture factory workers to microbial allegens associated with wood dust. Allergological examinations by skin and precipitin tests were performed in 48 workers employed in a factory producing furniture from fibreboards and chipboards, and in 32 healthy urban dwellers not exposed to organic dusts (referents). The skin test was performed by the intradermal method with the saline extracts of the cultures of 3 microbial species (Rahnella sp., Arthrobacter globiformis, Aspergillus fumigatus) associated with wood dust. Skin reactions were recorded after 20 minutes, 8 hours and 24 hours and graded 1 - 4, depending on the diameter of the reaction. The agar-gel test for the presence of precipitins in serum was performed with the extracts of 15 microbial isolates. The furniture factory workers showed a high skin response to the extracts of environmental microbes. The frequency of early grade 2 reactions (diameter 10 mm) to the extract of Rahnella sp. was 64.6 p.c. among furniture workers, being significantly higher (p 0.001) compared to reference group (18.7 p.c.). High frequencies of grade 2 reactions in furniture workers were also found with the extracts of A. globiformis and A. fumigatus (52.1 p.c. and 62.5 p.c., respectively). The frequencies of grade 2 delayed (after 8 h) and late (after 24 h) reactions to Rahnell sp. in furniture workers were non-specifically high (97.9 p.c./93.7 p.c.) while the response rates to A. globiformis and A. fumigatus were much lower (10.4 p.c./25.0 p.c., and 4.2 p.c./37.5 p.c., respectively). In agar-gel test for detection of precipitins, in most cases very low percentages...

541/2523

Tytuł oryginału: Work-related symptoms among furniture factory workers in Lublin region (eastern Poland).
Autorzy: Milanowski Janusz, Góra Anna, Skórska Czesława, Krysińska-Traczyk Ewa, Mackiewicz Barbara, Sitkowska Jolanta, Cholewa Grażyna, Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.99-103, tab., bibliogr. 24 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: 48 woodworkers employed in the furniture factory were examined. The control group consisted of 41 office workers with no exposure to organic dust. The examination included: interview on work-related symptoms, physical examination, and lung function test performed before and after the working-day. 38 out of 48 (79.2 p.c.) woodworkers reported work-related symptoms. The most common complaint was dry cough reported by 25 workers (52.1 p.c.), followed by general malaise - reported by 17 (35.45 p.c.), cojunctivitis - by 16 (33.3 p.c.), rhinitis - by 16 (33.3 p.c.), and skin symptoms by 16 (33.3 p.c.). Other symptoms such as headache, shortness of breath and chest pain occurred less frequently. Subjects working in initial processing and board processing departments had a higher prevalence of cough compared to workers employed in the varnishing depertment (p 0.01). The prevalence of skin symptoms was significantly higher in board preocessing and varnishing departments compared to initial processing department (p 0.05). Occupational asthma and allegic alveolitis were recorded in 3 out of 48 (6.2 p.c.) and 2 out of 48 (4.2 p.c.) workers, respectively. Baseline FVC adn FEV1 values were lower in woodworkers compared to controls (p 0.01). The increased lung function parameters (FVC, FEV1) were observed in woodworkers who smoked compared to non-smokers. The difference was not statistically significant. There was a significant over-shift decrease of all measured spirometric values: FVC, FEV1, FEV1/VC, PEF among woodworkers (p 0.001). There was a significant pre-shift, post-shift decline...

542/2523

Tytuł oryginału: Study on the occurrence of Borrelia burgdorferi sensu lato and tick-borne encephalitis virus (TBEV) in ticks collected in Lublin region (eastern Poland).
Autorzy: Cisak Ewa, Chmielewska-Badora Jolanta, Rajtar Barbara, Zwoliński Jacek, Jabłoński Leon, Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.105-110, il., tab., bibliogr. 35 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: In 114 Ixodes ricinus ticks from 1 district of Lublin region (eastern Poland) exmine by dark field microscopy method, the presence of motile spirochetes, morphologically corresponding to Borrelia species was detected in 8.8 p.c. of the total examined ticks. The highest infection rate was noted among females (16.7 p.c.), much lower in males (7.1 p.c.) and nymphs (4.0 p.c.). Examination of 550 ticks collected from 3 districts of the Lublin region by polymearse chain reaction (PCR) showed that 5.3 p.c. of the total number of ticks were infected with Borrelia spirochetes. The highest rates of Borrelia infection were observed in Ixodes ricinus ticks from the Zamość and Lublin districts (9.6 p.c. and 4.7 p.c. respectively). In the Włodawa district, only 2.4 p.c. ticks showed the presence of Borrelia DNA. In contrast to the results obtained by dark field microscopy method, the highest infection rate was noted in males (11.2 p.c.), followed by females (6.9 p.c.) and nymphs (1.7 p.c.). 57 Ixodes ricinus ticks collected from 3 Lublin districts (Lubartów, Lublin, Radzyń Podlaski) were tested for the presence of tick-borne encephalitis virus (TBEV) by the inoculation of 5-week-old Swiss mice, followed by blind passages and inoculation of GMK cell culture with brain suspension of the infected mice. On strain of TBEV was isolated from a pool of 24 I. ricinus ticks collected from the Radzyń Podlaski district. The minimum infection rate of ticks from this district with TBEV was estimated as 4.2 p.c, and in the total area of Lublin region estiamted as 1.8 p.c.

543/2523

Tytuł oryginału: Longterm decrease in the CD57 lymphocyte subset in a patient with chronic Lyme disease.
Autorzy: Stricker Raphael B., Burrascano Joseph J., Winger Edward E.
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.111-113, tab., bibliogr. 5 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Lyme disease is a tickborne illness caused by the spirochete Borrelia burgdorferi. In a previous report we described a decrease in the CD57 lymphocyte subset in patients with chronic Lyme disease. We have now identified a patient with chronic relapsing and remitting symptoms of Lyme disease who had decreased levels of CD57 lymphocytes over 10 years. This observation represents the longest duration of an immunologic abnormality ever documented in chronic Lyme disease. The CD57 lymphocyte subset appears to be a useful marker of longterm infection with the Lyme disease spirochete.

544/2523

Tytuł oryginału: Allergic contact urticaria and rhinitis to roe deer (Capreolus capreolus) in a hunter.
Autorzy: Śpiewak Radosław, Dutkiewicz Jacek
Źródło: Ann. Agric. Environ. Med. 2002: 9 (1) s.115-116, bibliogr. 11 poz.
Sygnatura GBL: 313,158

Hasła klasyfikacyjne GBL:

otorynolaryngologia

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Roe deer (Capreolus capreolus) is one of the most common game mammals in Europe, where hundreds of thousands people are exposed to this antimal. Despite this fact, we are aware of only two cases of allergy to roe deer published until recently, one case of allergic rhinoconjunctivitis and asthma and the second of contact urticaria. We describe another case with co-existing allergic contact urticaria and rhinitis in a 55-year old male professional hunter. The symptoms were provoked only by exposure to roe deer, and there were no other past or present allergic diseases. Specific IgE was found to following animal allergens: cow dander (CAP class 5), goat epithelium and horse dander (each CAP class 4), dog epithelium, dog dander and swine epithelium (each CAP class 2). Skin prick tests have shown positive raction only to cow epithelium (+). Because of lack of deter dander allergen for specific IgE and skin tests, we have confirmed the causal relationship between exposure to roe deer and allergy using the rub test with roe deer's fur. There was a clearly positive urticarial reaction on the patient's skin accompanied by nasal itch, steezing and rhinorrhea. No reaction was seen in a control person. We surmise that the positive tests with cow epithelium seen in this patient may result from a cross-reactivity to deer allergens. We conclude that although occupational allergies to roe deer seem to be rate, such possibility should be always considered among people having contact with these animals.

545/2523

Tytuł oryginału: A newly discovered function of palatine tonsils in immune defence: the expression of defensins.
Tytuł polski: Nowo odkryta funkcja immunologiczna migdałka podniebiennego obrony.
Autorzy: Weise J. B., Meyer J. E., Helmer H., Wittrock H., Maune S.
Źródło: Otolaryngol. Pol. 2002: 56 (4) s.409-413, il., tab., bibliogr. 22 poz., streszcz.
Sygnatura GBL: 313,466

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Migdałek podniebienny niewątpliwie pełni rolę w systemie obrony immunologicznej. Po kontakcie z antygenem uwalniane są limfocyty B i T jako efekt odpowiedzi immunologicznej. Dodatkowo migdałek podniebienny wydaje się wpływać na funkcje obronne poprzez aktywację systemu immunologicznego. Dlatego zbadaliśmy zdolność migdałka podniebiennego do zróżnicowania obrony immunologicznej typu alpha i beta w przewlekłym procesie zapalnym. Wyizolowano RNA pochodzące z 49 hiperplastycznych lub będących w stanie przewlekłego zapalenia migdałków podniebiennych, w których stwierdzono obecność Actinomyces israeli. Przeprowadzono analizę ekspresji genów HNP-1, HNP-4, HBD-1, HBD-2 za pomocą standaryzowanej reakcji glicerynoaldehyd-3-fosfodehydrogenazą po wykonaniu półilościowej PCR. mRNA HNP-1, HNP-4, HBD-1,, HBD-2 zostało wykryte w próbkach tkankowych, lecz ich ilość różniła się w dwóch typach obrony i w tkankach o różnym pochodzeniu. HBD-1 zostało stwierdzone we wszystkich 49 przypadkach przewlekłego zapalenie i w hiperplastycznych migdałkach podniebiennych. Poziom mRNA HBD-2 był istotnie podniesiony tylko w przewlekłym zapaleniu migdałków. Ekspresja mRNA migdałków podniebiennych w różnych typach obrony immunologicznej alpha i beta sugeruje nową funkcję migdałka w systemie odpowiedzi immunologicznej: udział we wrodzonym, nieadaptowanym systemie immunologicznym. Dlatego migdałek podniebienny ma potencjalnie wpływ na wzrost i kontrolę fizjologicznej flory bakteryjnej poprzez swoją ...

Streszczenie angielskie: The palatine tonsils have an undoubted role in the immune defence system. After antigen contact an effective adaptive immune response aby B-and T-cell lymphocytes will be released. In addition the palatine tonsils seem to exert influence to the defence by the innate immune system. Therefore, we studied the ability of palatine tonsils to express different alpha and beta defencsins and to find out any distinctions in chronic inflamed tonsils. Total RNA of 49 specimens of hyperplastic tonsils and chronic tonsilitis with pathological provided evidence of Actinomyces israelii was islolated using TRIzol protocol, reverse transcribed and the HNP-1, HNP-4, HBD-1 and HBD-2 gene expression densitometric determined, standardised in relation to glycerinaldehyd-3-phosphatdehydrogenase gene expression, after a semiquantitative polymerase chain reaction was performed. mRNA of HNP-1, HNP-4, HBD-2 was detected in tissue samples, but their amount differed within the two defensin families and tissue of origins. HBD-1 was detected in all 49 tissues of hyperplastic tonsils and chronic tonsilitis. Only in chronic inflamed tonsils the amount of HBD-2 mRNA expression was significant increased. In these specimens also mean relative expression rate of all defensins was observed to be manifestly increased. Palatine tonisls express mRNA for different alpha and beta defensins and this expression suggest a newly supposed function in immune defence: the participation in the innate, non-adaptive ...

546/2523

Tytuł oryginału: Modulation of the susceptibility of intestinal bacteria to bacteriophages in response to Ag43 phase variation - a hypothesis.
Autorzy: Węgrzyn Grzegorz, Thomas Mark S.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.HY15-HY18, bibliogr. 38 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Escherichia coli is a Gram-negative bacterium which colonises the intestinal tract of man and other animals. In addition to being a part of the normal bacterial flora of the human intestine, there are a number of enteropathogenic strains of E. coli which cause infections ranging in consequence from diarrhoea to colitis. Antigen 43 (Ag43) is the major phase-variabale protein in the outer membrane of E. coli. One benefit for bacteria resulting from phase variation of surface antigns is usually ascribed to evasion of host defences. However, results of recent studies indicate that infection of E. coli by different bacteriophages is inhibited in the presence of certain bile salts and carbohydrates (components present in the human intestine but absent in standard bacteriological media) when cells are in the "OFF" state for production of Ag43. The inhibition of bacteriophage development was found to be due to a significant impairment in the process of phage adsorption and evidence was presented for the binding of phage to Ag43. Here we present a hypothesis that in the case of Ag43, phase-variation might benefit the host bacterium by modulating the susceptibility to phage infection in the gut. If this hypothesis is true, it may have important implications not only for basic research but also for development of bacteriophage therapy, a re-discovered method of treatment of patients with infectious diseases.

547/2523

Tytuł oryginału: Cyclic nitric oxide release by human granulocytes, and invertebrate ganglia and immunocytes: nano-technological enhancement of amperometric nitric oxide determination.
Autorzy: Stefano George B., Salzet Michel, Magazine Harold I.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR199-BR204, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Various tissues from vertebrates and invertebrates respond to external signal molecules by rapid release of nitric oxide (NO) mediated by constitutive nitric oxide synthase. Invertebrate immunocytes were collected from maintained stock and human granulocytes were isolated from leukocyte-enriched blood obtained from the Long Island Blood Services. The invertebrate ganglionic tissue was either extracted or exposed for ex vivo and in vivo evaluation. Nitric oxide release was measured using a newly developed NO-selective nanoprobe, exhibiting enhanced sensitivity. Evaluation of NO release from the pedal ganglia of the maring bivalve, Mytilus edulis, demonstrated in vitro release of NO that fluctuated from 969 to 1003 pM, with a mean change in NO of 35 pM/cycle and a mean cycle time of approximately 4 minutes. Basal release of NO/cycle from the ganglia in vivo was increased significantly to approximately 65 pM (P 0.05) with an increase in cycle time to approximately 7 minutes. Exposure of the ganglia to morphine in vivo resulted in a significant increase in NO release and a lack of NO pulsations. The fluctuation in NO release from immunocytes of Mytilus edulis was approximtely 27 pM per cycle with a cycle time of 4 minutes whereas human granulocytes release fluctuated approximately 23 pM with a cycle time of 6 minutes. Theses data demonstrate that basal release of NO from various tissues is released in a cyclic manner and the cycle time and magnitude is subject to regulation by external stimuli.

548/2523

Tytuł oryginału: Effect of anti-oxidants, free radical quenchers and cyclo-oxygenase inhibitor on benzo(a)pyrene-induced suppression of human lymphocyte mitogenesis in vitro.
Autorzy: Das Undurti N.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR205-BR207, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Benzo(a)pyrene (BP) is a known carcinogen and immunosuppressor. Activation of BP to its reactive intermediates is cyclo-oxygenase (COX) dependent and occurs via a free radical dependent mechanism. The aim of this study was to know whether anti-oxidants, free radical quenchers and (COX) inhibitor, indomethacin, influence the suppression of human T cells induced by BP in vitro. Human lymphocytes, obtanined from healthy human volunteers, were stimulated to proliferate using phytohaemagglutinin (PHA). The effect of vitamin E. SOD, catalase, mannitol, and COX-inhibitor, indomethacin, on BP-induced suppression of human T cell proliferation was evaluated. Except for catalase, a hydrogen peroxide (H2O2) radical quencher, none of the agents tested could inhibit BP-induced suppression of human T cell proliferation in vitro. BP-induced suppression of human T cell proliferation is probably, induced by increased production of H2O2. Prostaglandins and superoxide anion and hydroxyl redical do not seem to participate in BP-induced human T cell growth suppression.

549/2523

Tytuł oryginału: Effects of immunomodulators on acute Trypanosoma cruzi infection in mice.
Autorzy: Oz Helieh S., Hughes Walter T., Thomas Elaine K., McClain Craig J.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR208-BR211, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Patients who recover from acute trypanosomiasis may succumb to chronic Chagas' disease later in life due to age related immunosuppression, immune system disorders such as AIDS, or during periods of immunosuppressive therapy for organ transplantation. In this study, effects of immunomodultors with diverse properties were examined on the course of an acute and lethal Trypanosoma cruzi infection. ICR (Swiss) mice inoculated with Tulahuen (lethal) strain of T. cruzi were treated with 6 different immunomodulators and the course of infection was studied. Tacrolimus (FK-506) and dexamethasone increased parasitemia in mice when compared to infected untreated animals. Mycophenolate mofetil (RS-61443) ana recombinant interleukin-15 (IL-15) treatment decreased the number of parasites but had no effect on animal survival. In contrast, compound L-685-818 (tacrolius analog) and CD40 ligand (CD40L), provided protection against lethal infection. Mice that survived initial infection were all protected against reinfection. This study demonstrates the dangers of immunosuppression with tacrolimus and dexamethasone in T. cruzi infected subjects. While mycophenolate did not exacerbate the infection, our data suggest potential therapeutic applications for L-685-818 and CD40 ligand in trypanosomiasis.

550/2523

Tytuł oryginału: Chemoprotection profiles of sodium thiosulfate on methyl methanesulfonate-induced mutagenesis of bacteriophage T4.
Autorzy: Malik Ajmaluddin, Khan Asad Ullah, Lal Sunil Kumar
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR212-BR220, il., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The alkylation of nucleic acids is primarily responsible for chemical carcinogenesis. Even during disease treatment, several alkylating drugs interact with nucleic acids and cause severe toxic effects. Thus good chemoprotectants are necessary. For our study we chose a simple model organism, bacteriophage T4 (a nucleoprotenic particle), and alkylating agent methyl methanesulfonate (MMS) to study its lethal effects. Sodim thiosulfate (STS), used as a chemoprotectant, has been tested against alkylating drugs. Bacteriphage T4Do were exposed to different molarities of MMS for several pre-termination incubations. Alkylation reactions were stopped with different concentrations of STS at given pre-termination incubation periods and further incubated up to 24 hours. The viability (survival frequency) of phage T4 was studied at various post-termination intervals by plaque count assay. Our results show that the survival frequency is strongly influenced by MMS dosage and exposure time. However, the antidotal effect of STS on MMS-induced lethality directly corresponds to STS dosage. Survival frequencies with 1 p.c. quench solution were lower than with 5 p.c. quench solution at all molarities of MMS and at different pre-and post-termination periods. Our studies confirmed the role of STS in the cytoprotection of bacteriophage T4. In the presence of 1 p.c. STS, a moderate inhibition in cytotoxicity was observed, while 5 p.c. STS exhibited a significant inhibition against the cytotoxic activity of MMS, presumably due to a rapid covalent binding of the methyl group (carbocation - an electrophile ) of MMS with the nucleophilic sulfur atom of STS.

551/2523

Tytuł oryginału: Human leptin administered intraperitoneally stimulates natriuresis and decreases renal medullary NA+, K+-ATPase activity in the rat - impaired effect in dietary-induced obesity.
Autorzy: Bełtowski Jerzy, Wójcicka Grażyna, Górny Dionizy, Marciniak Andrzej
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR221-BR229, il., bibliogr. 37 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Leptin, produced by adipose tissue, apart from regulating food intake and energy expenditure, also has natriuretic activity. In this study we examined the effect of leptin on renal Na+, K+-ATPase responsible for active tubular sodium reabsorption, and compared the renal effects of leptin in lean and obese rats. Male Wistar rats were either kept on normal laboratory chow or made obese by a high-calorie diet. The animals were placed in metabolic cages and urine was collected in 2-hour periods. In lean animals, leptin (1 mg/kg i.p.) caused a 139.5 p.c. increase in uring output, a 112.4 p.c. increase in natriuresis, and a 57.2 p.c. increase in the fractional excretion of sodium, but had no effect on the glomerular filtration rate. Leptin at this dose decreased renal medullary Na+, K+-ATPase activity at 30 minutes, 1 hour and 2 hours by 31 p.c., 34.3 p.c. and 21.2 p.c., respectively. The effect of leptin on Na+, K+-ATPase at 1 hour was dose-dependent; the lowest dose inducing significant inhibition was 0.25 mg/kg. By contrast, leptin had no effect on either cortical Na+, K+-ATPase or the ouabain-resistant fraction of ATPas. In obese rats, leptin increased urine output by only 29.1 p.c. and natriuresis by 28.9 p.c., and had no significant effect on medullary Na+, K+-ATPase. Leptin stimulates natriuresis primarily by inhibiting tubular sodium reabsorption. This effect is mediated, at least partially, by decreased Na+, K+-ATPase actaivity in the ranal medulla, and is impaired in obese rats.

552/2523

Tytuł oryginału: Human cytomegalovirus infection and abortion: an immunohistochemical study.
Autorzy: Spano Liliana Cruz, Pereira Fausto Edmundo Lima, Basso Nadia Gomes da Silva, Mercon-de-Vargas Paulo Roberto
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR230-BR235, il., tab., bibliogr. 50 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Cytomegalovirus (HCMV) has been described in abortion tissues, but seldom associated with inclusion bodies. However, the possible pathogenic role of this virus in aborton is under discussion. We attempted to verify the realtionship between HCMV antigens in tissues from first trimester abortions presenting with inflammatory lesions. Sixteen cases of first trimester abortions with inflammatory lesions were selected from 340 cases studied at the Pathology Unit of the University Hostoptal in Vitória, Espirito Santo State, Brazil. Paraffin-embedded sections were subjected to indirect immunofluorescence (IFI) and immunoperoxidase (PAP), using monoclonal antibodies directed against immediate early (IEA) and late antigens (LA) of HCMV. Twelve out of sixteen cases (75 p.c.) presented at least one HCMV antigen. Eight cases presented both antigens, three cases only the IEA and one case only the LA. These antigens were present in decidual cells, in stromal cells of chorionic villi and in trophoblastic cells. The results showed high frequency of HCMV antigens in tissues from first trimester abortion. This frequency, higher than that previously reported, was probably due to the fact that necroinflammatory lesions were always present in selected cases. The presence of LA in trophoblastic cells is evnce for cell permissiveness to viral replication in vivo. The results showing high presence of HCMV antigens in tissues from abortion with inflammatory lesions suggest a possible relationship of HCMV infection with inflammation and pregnancy loss.

553/2523

Tytuł oryginału: Effect of ambroxol on ion transport - a model study.
Autorzy: Kosik-Bogacka Danuta I., Tyrakowski Tomasz
Źródło: Med. Sci. Monitor 2002: 8 (6) s.BR236-BR241, il., tab., bibliogr. 44 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Żaby

Streszczenie angielskie: Effect on ambroxol on reflexive physiological reactions of isolated frog skin was studied with elctrophysiological methods designed to assess on currents occurring in epithelial tissues or organs. The experiments permitted to analyze an effect of ambroxol on regulatory mechanisms, which in turn influence the constant electrical potential defined as PD and reversible changes of this potential during mechanical stimulation (marked as dPD). A contribution of sodium- and chlorid eions transport to the value of transepithelial electrical potential of frog skin (before adn after ambroxol administration) has been demonstrated using amiloride - a blocker of sodium ion transport and bumetanide - a blocker of chloride ion transport. The experimental material comprised 34 fragments of hybrid frog (Rana esculenta L.),, representing 14 spcimens. The experiments involved measuring the transepthelial electrical potential (PD in mV) on an isolated frog skin place in an Ussing apparatus. It has been demonstrated that ambroxol influences the processes of ion transport (dPD) which in turn depend on mechanical stimulation. The reaction of hyperpolarization decreased immediately after administration of ambroxol to the stimulating fluid, when transepithelial transport of sodium ion was maintained in the preparations studied. Consequently, no efect of ambroxol on the frog skin incubated wtih amiloride was recorded. It has been demonstrated, based on the experiments carried out in the present study , taht ambroxol can modify processes of ion transport related to activation of sensory receptors...

554/2523

Tytuł oryginału: Intervention in patients with pneumothorax immediately following CT-guided fine needle aspiration of pulmonary nodules.
Autorzy: Shantaveerappa Harsha N., Mathai Mathew G., Byrd jr Ryland P., Karnad Anand B., Mehta Jayant B., Roy Thomas M.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR401-CR404, tab., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The incidence of pneumothorax (PTX) as a complication of computerized tomography guided fine needle aspirates (CT-FNA) of solitary pulmonary nodules (SPN) varies from 8 - 61 p.c. It has been suggested that the practice of obtaining a delayed chest radiograph in patients who have undergone CT-FNA of SPN is not cost effective and adds little information concerning lung expansion obtained by CT and the end of the procedure. It, however, is not known what percent of patients with a PTX present immediately after CT-FNA but do not require prompt chest tube placement will progress and require intervention later. One hundred-fifty-eight consecutive patients undergoing CT-FNA of SPN were included in the study. Immediately after CT-FNA each patient was reimaged with the CT scanner to checka for PTX. Patients with a PTX immediately after CT-FNA were assessed as to whether intervention was undertaken or whether the PTX enlarged and/or required drainage at a later time. Thirty-eight patients developed a PTX while still on the CT scanner. Twenty-nine patients with an immediate PTX did not require drainage of their pleural space. Chest tube placement was required prompty after the CT-FNA in 4 patients. Five patients had their pleural space drained at a later time due to an increasing size of the PTX and/or the development of symptoms attributed to the PTX. These data suggest that patients who develop a PTX immediately after CT-FNA but who do not require prompt pleural space evacuation should be followed closely both clinically and radiographically.

555/2523

Tytuł oryginału: Dyspnea scales as a measure of health-related quality of life in patients with idiopathic pulmonary fibrosis.
Autorzy: Martinez Jose Antonio Baddini, Martinez Tieko Yamamoto, Galhardo Fabiola Paula Lovetro, Pereira Carlos Alberto de Castro
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR405-CR410, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Idiopathic pulmonary fibrosis (IPF) is the most common interstitial lung disease and is usually associated with a poor prognosis. IPF patients have a significant impairment in health-related quality of life (HRQL), both in terms of physical and psychological functioning. A substantial number of dyspnea scales are available to clinicians for clinical and research use. The objective of the present study was to investigate how some commonly used dyspnea scales correlate with measurements of HRQL in IPF patients. A group of 30 patients with IPF who attended a specialized outpatient respiratory clinic were interviewed during a stable period of the disease. HRQL was assessed by the SF-36 questionnaire. Dyspnea was measured by the baseline dyspnea index (BDI), Modified Medical Research Council Scale (MRC), Oxygen Cost Diagram (OCD), clinical element of the Clinical, Roengenographic and Physiologica score (CRP), and a new dyspnea scale (NS) based on estimates of required oxygen consumption for usual activities. Patients showed impariments in all HRQL domains except the Pain Index. All dyspnea scales showed significant correlations with both physical and mental HRQL domains. The highest Spearman's coefficients between dyspnea ratings and SF-36 components were obtained using the BDI. Dyspnea ratings correlate with HRQL measurements in IPF patients. Dyspnea scales can be used as a measure of HRQL in special circumstances.

556/2523

Tytuł oryginału: Further arguments against including trisialo-Fe2-transferrin in carbohydrate-deficient transferrin (CDT): a study on male alcoholics and hazardous drinkers.
Autorzy: Arndt Torsten, Korzec Aleksander, Br Marij, Kropf Jrgen
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR411-CR418, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: We attempted to determine whether including trisialo-Fe2-transferrin in carbohydrate-deficient transferrin (CDT) affects the diagnostic accuracy of CDT as a marker of chronic excessive alcohol intake. The criterion standard tests for the diagnosis of alcoholism and alcohol intake were the Composite International Diagnostic Interview (CIDI) and the Timeline-Followback (TLFB). The study groups (alcohol intake in each of the last 4 weeks before blood sampling) were comprised of 56 controls (ó 280 g/week, no alcoholism), 54 hazardous drinkers ( 280 g/week, no alcoholism), 63 alcoholics ( 280 g/week, alcoholism diagnosis). CDT analysis was performed with p.c.CDTri-TIA, which includes about 50 p.c. of trisialo-Fe2-transferrin in CDT, and ChronAl-col. D, which excludes this transferrin isoform from CDT. Depending on the cut-offs for the CTD/transferrin ratio (upper or lower limit of the test-specific borderlines) and on the patient group, the diagnostic sensitivity was 18.1 p.c. - 72 p.c. for p.c.CDTr-TIA, as opposed to 50.p.c. - 82.5 p.c. for ChronAlcol.D. The diagnostic accuracy was 62.8 p.c. - 78.5 p.c. for p.c.CDTri-TIA and 71.8 p.c. - 86.6 p.c. for ChronAlcol.D. The latter test consistently showed higher diagnostic sensitivity and accuracy than p.c. CDTri-TIA. The diagnostic specificity was 85.7 p.c. - 98.2 for p.c.CDTri-TIA and 91.1 p.c. - 92.2 p.c. for ChronAlcol.D. The areas under the ROC curve were 0.810 p.c. - 0.885 for p.c.CDTri-TIA and 0.867 p.c. - 0.896 for ChronAlcol.D. The present study and data from the literature indicate that including parts of trisialo-Fe2-transferrin by the p.c. CDRri-TIA test significantly reduces the diagnostic sensitivity and thus accuray of CDT as a marker of chronic excessive alcohol use.

557/2523

Tytuł oryginału: Oxidative stress in the blood of patients with alcohol-related liver cirrhosis.
Autorzy: Szuster-Ciesielska Agnieszka, Daniluk Jadwiga, Kandefer-Szerszeń Martyna
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR419-CR424, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Ethanol may increase the production of reactive oxygen species (ROS) in the liver, which results in the development of alcoholic liver disease (ALD). Cytokine-acitvated blood leukocytes may also participate in this process. The aim of our study was to evaluate the oxidative stress level in the blood of patients with alcoholic liver cirrhosis. Blood neutrophils from 16 patients with compensated alcoholic liver cirrhosis and 28 patients with decompensated were evaluated for their ability to produce superoxide anion and hydrogen peroxide spontaneously and after PMA induction, in comparison to controls (16 healthy persons). Serum catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity were also measured in both groups of patients and in the controls. The neutrophils from patients with compensated liver cirrhosis spontaneously produced a normal level of Oz- and a high level of H2O2, but exhibited a defect in PMA-induced O2- production. The neutrophils from patients with decompensated liver cirrhosis spontaneously produced more O2- adn H2O2 than the controls, but the PM response was weak. There were no changes in the expression of GPx, buth enhanced SOD activity was observed in the serum of patients with decompensated alcoholic liver cirrhosis. Increased CAT activity was detected only in serum from patients with compensated liver cirrhosis. These data point to oxidative stress in the blood of patients with decompensated alcoholic cirrhosis, since increased resting production of ROS in neutrophils was not accompanied by increased GPx and CAT activity in serum.

558/2523

Tytuł oryginału: Atherosclerosis risk factors in children and adolescents with or without family history of premature coronary artery disease.
Autorzy: Kelishadi Roya, Zadegan Nizal Sarraf, Nadery Gholam Ali, Asgary Sedigueh, Bashardoust Nasrollah
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR425-CR429, tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Although coronary artery diseases (CAD) becomes symptomatic much later in life, the early identification and modification of risk factors may reduce its later incidence. 100 subjects 2 - 18 years old, evenly divided by sex, were randomly selected from among children of patients suffering from premature myocardial infarction ( 55 years); the controls were 100 age- and sex-matched subjects without a similar family history. In the Pediatric Preventive Cardiology Clinic at the Isfahan Cardiovascular Research Center, the subjects completed a special questionnaire consisting of anthropometric data, blood pressure, skinfold thickness, rate of physical activity, and active or passive cigarette smoking. Fasting venous blood was analyzed for serum lipids, lipoproteins fibrinogen, and apolipoproteins A1 and B100. The data were analyzed with SPSS V6/vin using the independent t-test, Kruskal-Wallis, chisquared, and standard linear multiple regression tests. The data showed higher prevalence of some major and new risk factors in the experimental group than in the controls. The mean total cholesterol, LDL-C, TG, fibrinogen and Apo B100 were significantly higher in the experimental group, while the mean values of HDL-C and Apo A1 were significantly lower. The differences in terms of Body Mass Index, percentage body fat, rate of regular physical activity, and active and passive smoking were not significant between groups. Major and new CAD risk factors should be identified and modified as early as possible in children with high family risk by screening and health education at an early age.

559/2523

Tytuł oryginału: The renin-angiotensin-aldosterone system (RAAS) and its relation with calcium homeostasis in male obesity.
Autorzy: Ozata Metin, Durmus Oziem, Yilmaz Mahmut Ilker, Bolu Erol, Erdogan Murat, Ozdemir I. Caglayan
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR430-CR433, tab., bibliogr. 23 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of our study was to determine whether alterations of the vitamin D-endocrine system and the renin-angiotensin-aldosterone system occur in male obesity and influence each other. 32 ibese male subjects and 21 age-matched healthy male subjects were enrolled in the study. The parameters measured included serum total calcium (Ca), ionized calcium, magnesium (Mg), phosphorus (P), 25-hydroxyvitamin D (25-OHD), supine and upright aldosterone and renin activity, and urinary calcium, magnesium, and phosphorus. Serum total Ca and ionized calicum were slightly, but not significantly lower in obese subjets compared to controls, whereas serum magnesium phosphorus and PTH levels were significantly higher in the obese subjects. Mean urinary calcium and Mg were not significantly different, while urinary P was significantly higher in obese subjects. Mean serum 25OHD was significantly lower in obese subjects. Supine and upright aldosterone and renin were unrelated to BMI or fat mass. Supine aldosterone correlated with serum total calcium, ionized calcium and supine renin activity, but not with other minerals, PTH or 25OHD, whereas upright aldosterone did not correlate with other parameters. Supine renin activity did not correlate with any parameters, whereas upright renin correlated with serum Mg an PTH. There were significant correlations between PTH and BMI or fat mass. Urinary Mg was also related to both BMI and fat mass. Male obesity is associated with increased serum Mg, P, PTH and urinary P and lower supine aldosterone and 25OHD, but unchanged serum and urinary Ca.

560/2523

Tytuł oryginału: Symptomatic annular pancreas in newborns.
Autorzy: Sencan Aydyn, Mir Erol, Gnsar Cneyt, Akcora Blent
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR434-CR437, tab., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

Streszczenie angielskie: The pancreas exhibits various types of anomalies, including aplasia, dysplasia, pancreatc cysts, duplication, and ectopis, of which the most common is annular pancreas. This stdy describes the characteristic features of 7 cases of annular pancreas diagnosed during exploration. Seven newborns undergoing surgery for annular pancreas from 1990 to 1998 were analyzed. The cases were evaluated according to birth weight, sex, symptoms, methods of diagnosis, associated anomalies, and surgical treatment modalities. The mean birth weight of the patients was 2385 ń 1002 g, the mean gestational age was 37.5 3.1 weeks. Four patients (42.8 p.c.) had associated anomalies, including intestinal malrotation (42.8 p.c.), intrinsic duodenal obstruction (28.5 p.c.), trisomy 21 karyotype (14.2 p.c.), cardiac malformation (14.2 p.c., and Meckel's diverticulum (14.2 p.c.). Surgical treatment included denoduodenostomy (DD) in four patients and DD plus tapering enteroplasty (TE) plus placement of transanastomotic jejunal tube (TJT0 in three patients. Infants with annular pancreas associated with duodenal obstruction are generally premature or small for their gestational age. The symptoms observed in annular pancreas ate related not only to extrinsic compression of the ectopic tissue, but also the duodenal stenosis associated with this malformation. Annular pancreas is most commonly associated with intestinal atressias, and oral feeding tolerance time is nearly the same between the DD and DD+TE+TJT groups.

561/2523

Tytuł oryginału: Extrahepatic bile duct injury during laparoscopic cholecystectomy - a survey of 6873 patients.
Autorzy: Paczyński Andrzej, Koziarski Tomasz, Stanowski Edward, Krupa Jarosław
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR438-CR440, bibliogr. 8 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Despite recognised advantages of laparoscopy, the damage of extrahepatic bile ducts during cholecystectomy is a subject of ongoing discussions, as such injuries are more frequent comparing with open surgery. The analysis included 6873 patients who underwent laparoscopic cholecystectomy. The study group comprised both the patients after elective surgery due to symptomatic cholelithiasis and patients after emergency procedures necessiated by acute cholecystitis. Common bile duct was damaged in 9 patients. Five people had their main bile duct transected, in 2 people it was partial excision, and in the other 2 subjects - puncture damage with electrocoagulation took place. In seven cases, the damage was diagnosed still during laparoscopy and conversion to open surgery was performed. In two subjects the damage was diagnosed in postoperative period and it was subsequently confirmed by ERCP. Roconstructive surgery included: BCD suturing with interrupted stiches, end-to-end anastomosis over T-tube and Roux-en-Y bilioenteric anastomosis. Good postoperative outcome was obtained in 6 patiens. One subject required repeated endoscopic dilatation and placement due to recurrent cholangitis. Two patients died due to upper gastrointestinal bleeding and multiple organ failure. Extrahepatic bile duct injury remains a dangerous complication following cholecystectomy. It is more likely to occur in case of anomalous anatomy of bile ducts, inflammatory or malignant infiltration and technical errors of an operating surgeon. It is very important to diagnose the damage early enough, preferably still during laparoscopy. Imaging investigations (ultrasound, ERCP, MRCP, cholangiography) may be helpful in establishing the diagnosis during the postoperative period.

562/2523

Tytuł oryginału: Apoptosis and pH of blasts in acute childhood leukemia.
Autorzy: Malinowska Iwona, Stelmaszczyk-Emmel Anna, Wąsik Maria, Rokicka-Milewska Roma
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR441-CR447, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The purpose of our study was to determine whether spontaneous and post-initial therapy apoptosis indexes (AI) and the intracellular pH of blasts have prognostic value in the treatment of children with acute leukemia. Blasts from 38 children with acute leukemia (31 with ALL and 7 with AML) were tested for apoptotic index (Annexin V) and intracellular pH (SNARF). Of the ALL patients, 9 were low risk, 7 medium risk, and 15 high risk. Among the AML patients, 1 was low risk and 6 were high risk. Follow-up ranged from 6 to 120 weeks (median 60 weeks). The maen spontaneous AI was 19 ń 16 p.c. The pH of leukemic blasts before treatment varied between 6.6 and 7.9 (mean 7.2 ń 0.5). For patients 10 years old, the markers for good prognosis were a WBC count below 50 x 10**3/ćl, good response to prednisone therapy at day 8, and remission at or before day 33. Univariate analysis showed that pH 7 had favorable prognostic significance. Overall, the probability of EFS for patients with pH 7 was 1.0, as opposed to 0.49 for those with pH 7 (p = 0.049, n = 36). The probability of EFS for patients with AI below the median was not significantly different from those with AI above the median (0.4 vs 1.0, NS). The assessment of intracellular pH in blasts may be an important prognosticator for ALL patients. Children with low spontaneous ALL or high pH of leukemic blasts appear to have an unfavorable prognosis.

563/2523

Tytuł oryginału: Pathological somatosensory evoked potentials in patients with hypertension: a contribution to the study of the pathophysiology of juvenile essential hypertension.
Autorzy: Varsik Pavel, Buranova Darina, Balaz Marek, Duris Ivan
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR448-CR452, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: The role of factors influencing the cerebral regulation of blood pressure must be considered in the etiopathogenesis of essential hypertension. The aim of our work was to ecplore the possibilities for differentiating certain clinical, functional and electrophysiological signs in young and adult hypertensives. This could help us to determine whether centrally active antihypertensive drugs may be more suitable than others. We examined somatosensory evoked potentials (latencies of wave deflections and amplitudes) in two groups of patients with essential hypertension. The first group consisted of 25 hypertensive patients aged 20 to 30 years with onset before age 20 (n = 25). The second group consisted of 40 hypertensive patients aged 40 to 60 years with onset after the age of 30. The results were compared with those obtained from age-correlated healthy controls. The most interesting changes were observed in the amplitudes of somatosensory evoked potentials in the group of juvenile patients with hypertension. The decrease in the amplitude of all wave deflections was very compact, consistent, and statistically significant even after repeated examinations. This finding raises the possibility of influence or damage to the peripheral afferentation of signals inuvenile patients, with hypertension as an expression of the actions of brainstem structures. Our results suggest that afferent impulses and the reactivity of brainstem structures may play a role in the etiopathogenesis of hypertension.

564/2523

Tytuł oryginału: Incidence and molecular analysis of glucose-6-phosphate dehydrogenase deficiency in the province of Denizli, Turkey.
Autorzy: Keskin Nazan, Ozdes Ibrahim, Keskin Ali, Acikbas Ibrahim, Bagci Huseyin
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR453-CR456, tab., bibliogr. 21 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: G6PD deficiency is a widespread abnormality of glucose-6-phosphate dehydrogenase, a red cell enzyme, which gives rise to hemolysis under oxidative stress. In Turkey, G6PD deficiency has a variable frequency in different regions. The prevalence and genotypes of G6PD deficiency are not known in Denizli province of the Aegean region of Turkey. Accordingly, this study was designed to investigate the prevalence of enzyme deficiency and the distribution of the Mediterranean mutation of G6PD in this region. A total of 1950 students (918 females, 1032 males, ages between 14 and 17) were screened by the Fluorescent Spot Test, and the G6PD deficiency was confirmed by quantitative spectrophotometric assay. The G6PD deficient subjects were further analyzed by the PCR/RFLP technique to identify the presence of the 563 T Mediterranean mutation. 24 of the subjects wre found to be deficient in this enzyme, a frequency of 1.23 p.c. Of 24 deficient subjects, 19 (79 p.c.) had the 563 T Mediterranean mutation. The frequency of G6PD enzyme deficiency apperars to be low compared with those found in the malaria-endemic Mediterranean region of Turkey. The molecular pathology of G6PD deficiency is related to the G5PD-563 T mutation in the Denizli region.

565/2523

Tytuł oryginału: Does the negative correlation found in breast cancer patients between plasma melatonin and insulin-like growth factor-I concentrations imply the existence of an additional mechanism of oncostatic melatonin influence involved in defense?
Autorzy: Kajdaniuk Dariusz, Marek Bogdan, Kos-Kudła Beata, Żwirska-Korczala Krystyna, Ostrowska Zofia, Buntner Barbara, Szymszal Jan
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR457-CR461, il., tab., bibliogr. 54 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Insulin-like growth factor-I (IGF-I) is probably involved in promoting both normal and neoplastic cell growth, neoplastic transformation processes, angiogenesis, and neoplasma progression. On the other hand, one possible mechanism of the oncostatic action of melatonin is its influence on the action and/or release of teh growth factors that stimulate neoplastic cell growth. Quantitative changes in melatonin and IGF-I, as well as an imbalance between melatonin and IGF-I, may affect the growth of breast cancer cells and exacerbate the disease. The aim of our research was to study the interactions between plasma melatonin and IGF-I concentrations in pre-menopausal breast cancer patients. Our research involved 24 breast cancer patients (mean age 43 ń 6) with stage II breast cancer (Bloom and Richardson classification), confirmed by histological studies, and were 4 weeks after radical mastectomy. The control group consisted of 16 healthy women wolunteers (mean age 44 ń 5). No statistically significant relations were found between mean plasma melatonin and IGF-I concentrations in the two study groups. In the breast cancer group the correlation coefficient between IGF-I concentration in plasma and melatonin was r = -0.058). The negative correlation between the plasma melatonin and IGF-I concentrations in the breast cancer patients we studied could rach statistical significance in a larger population. The presence of such a negative correlation between plasma melatonin and IGF-I concentrations in patients with neoplastic disease may imply the existence of an additional defense mechanism based on the oncostatic influence of melatonin.

566/2523

Tytuł oryginału: Presentation of intracranial arachnoid cysts in children: correlation between localization and clinical symptoms.
Autorzy: Mazurkiewicz-Bełdzińska Maria, Dilling-Ostrowska Ewa
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CR462-CR465, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: Ther present study was performed in order to determine the most common neurological signs of arachnoid cysts (AC) in a pediatric population and to evaluate if there is a correlation between the localization of the cyst and the clinical characteristics. Forty-five AC patients were studied, aged 2 - 17 years, who were consecutively referred to the Department of Developmental Neurology at the Medical University of Gdańsk between 1990 - 2001. We found that AC has a strong predilection to the temporal regions and was associated with epilepsy in 31 p.c. of the cases. The patient's main complaint, however, was headache (in 69 p.c. of casefs). In 6 cases AC required surgical treatment, because of intracranial hypertension. The AC concomitant with epilepsy had a significant prodilection to the left temporal region (92 p.c. of cases).

567/2523

Tytuł oryginału: Cardiotoxicity following different doses and schedules of 5-fluorouracil administration for malignancy - a survey of 427 patients.
Autorzy: Tsavaris Nicolas, Kosmas Christos, Vadiaka Maria, Efremidis Michalis, Zinelis Andreas, Beldecos Dimitris, Sakelariou Dimitris, Koufos Christos, Stamatelos George
Źródło: Med. Sci. Monitor 2002: 8 (6) s.PI51-PI57, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Although cardiotoxicity associated with 5-Fluorouracil (5-FU) administration is infrequent, there are case reports of acute coronary syndromes. We report on patients undergoing 5-FU chemotherapy who developed cardiac symptoms during its administration. In patients receiving 5-FU who experienced cardiac-related symptoms, ECG and serum cardiac enzyme determination were performed. If cardiotoxicity was detected, 5-FU infusion was interrupted, and the patients received sublingual nitrates and caradiac monitored in a coronary care unit for at least 72 hours. In cases of acute myocardial infarction, 5-FU was terminated Of 427 patients entering the study, 17 (4 p.c.) developed clinical symptoms and ECG abnormalities indicating 5-FU cardiotoxicity. Patients with continuous infusion (c.i.) of 5-FU had a higher incidence of cardiotoxicity (12/197, 6 p.c.) than the remaining (5/235, 2.1 p.c.) (p = 0.067), but more toxicity was enccountered in patients with c.i. of 5-fu + LV for 24 hours for 5 days than in patients with the same regimen of 5-FU without LV (p 0.027) or patients with short 5-FU + LV administration (p = 0.024). Seven of the 17 patients with 5-FU cardiotoxicity had an acute myocardial infarction, 4 developed ischemic changes, while 4 more patients had ECG abnormalities consistent with coronary vasospasm, of whom one subsequently died. The present study supports the toxic effect of 5-FU on myocardium which is largely schedule-dependent. Considerable vigilance is required when usnig this drug, and its toxic effect on the coronary endothelium and myocardium merit further investigation.

568/2523

Tytuł oryginału: Salvage chemotherapy with the gemcitabine/docetaxel combination in non-small cell lung cancer: an overview of recent phase II studies.
Autorzy: Kosmas Christos, Tsavaris Nicolas, Kalofonos Haralambos P.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.PI58-PI63, tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Docetaxel has been the only single agent with proven activity and documented survival benefit in the second-line treatment of advanced/refractory non-small cell lung cancer (NSCLC). Combinations of docetaxel with other active agents in this setting, such as gemcitabine, besides their popularity as front-line treatment, are currently being explored in an attempt to improve the results over single-agent docetaxel in relapsed/refractory NSCLC. Given the established activity of single-agent decetaxel in two recent large randomized phase III trials against best supportive care or versus single-agent Vinorelbine or Ifosfamide in patients with platinum pretreated NSCLC, and the proven activity of single-agent gemcitabine in this setting, combination regimens employing these two agents in various doses and schedules have recently been initiated. Adequately designed phase II studies using standard criteria of efficacy and safety, which peer-review based publication, were selected from the literature. The gemcitabine/docetaxel combination regimens employing these two agents in various doses and schedules have recently been initiated. Adequately designed phase II studies using standard criteria of efficacy and safety, with peer-review based publication, were selected from the literature. The gemcitabine/docetaxel combination in various schedules with or without G-CSF support as salvage therapy of NSCLC pre-treated with platinum + paclitaxel - based regimens has been evaluated in four recently published phase II clinical studies, and has been shown to represent a tolerable and active regimen in this setting, yielding a 10 - 33 p.c. response rate, improvement of disease-related symptoms, and meaningful median and 1-year survival figures in the...

569/2523

Tytuł oryginału: Case presentation of gastrinoma combined with gastric carcinoid with the longest survival record - Zollinger-Ellison syndrome: pathophysiology, diagnosis and therapy.
Autorzy: Konturek Stanisław J., Konturek Piotr C., Bielański Władysław, Lorens Krzysztof, Sito Edward, Konturek Jan W., Kwiecień Sławomir, Bobrzyński Andrzej, Pawlik Teresa, Karcz Danuta, Areny Hany, Stachura Tomasz
Źródło: Med. Sci. Monitor 2002: 8 (6) s.CS43-CS59, il., bibliogr. 129 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Zollinger-Ellison syndrome is a very rare disease caused by tumor with gastrin producing cells accompanied by hypergastrinemia leading to gastirc hypersecretion and peptic ulcers and their complications. Female case of gastrinoma (Zollinger-Ellison syndrome; Z-E) with a record of 38 yrs of survival. Acute gastro-duodenal ulcers started at 28 yr of age and Z-E was diagnosed by using gastrin assays. Basal and maximal acid outputs and ratio of basal/maximal outputs were away over normal limits. Because of ulcer recurrence and complications, patients was subjected to several gastric surgeries but refused total gastrectomy. She was also treataed with many H2-receptor (R) antagonists and proton-pump inhibitors (PPI), each new drug being initially highlyt effective but then showing declinig efficacy except when PPI, lansoprazole was used. The gastrin level rose in the course of disease from initial high value of 2000 pg/mL to the extreme 4500 ng/mL at present. During the last 2 yrs, metastasis mainly to liver developed and they were successfully treated by synthetic octapeptided derivative of somatostatin and, as a result, metastatis partly reduced and plasma gastrin drasticly decreased. Biopsy taken from liver metastasis showed the presence of typical gastrinoma cells with gastrin and chromogranin, while that from oxyntic mucosa revealed the ECL-cell hyperplasia with corcinoid tumors and unexpected gastric atrophy. This phenomenal case described in this article might be the new proven evidence needed by gastroenterologists to overturn the traditional treatemnt using total gastrectomy as a treatment of choice to the partial gastrectomy combined with proton pump inhibitors.

570/2523

Tytuł oryginału: General model to describe the structure and dynamic balance between different human serum lipoproteins and its practical application.
Autorzy: Tuzikov Fedor V., Tuzikova Nataliya A., Galimov Ravil V., Panin Lev E., Nevinsky Georgy A.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.MT79-MT88, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Many dangerous diseases are associated with changes in the concentration of blood lipoiproteins (LPs). Thus a fast and accurate method is needed to determine the composition of lipoprotein fractions in human serum. A comparison of 30 parameters characterizing different LPs in serum from 120 healthy donors and 102 multiple sclerosis patients was carried out using a unique algorithm developed to determine the concentrations of all the main lipids and apolipoproteins in each LP fraction and subfarction. Specially developed computer programs and the small-angle X-ray scattering (SAXS) method were used to analyze the literature and experimental data. A general mathematical model has been developed to descrie the structure and equilibrium between various LPs, from high density to chylomicrons. All human serum LPs can be regarded as spherical particles, composed of a lipid hydrophobic spherical core consisting of triglycerides and cholesterol esters, and a hydrophilic shell of free cholesterol, phospholipids and apolipoproteins. We show for the first time that the distribution of components among various LP particles can be described by a system of five basic equations and two additional balance equations. The observed difference between control subjects and MS patients was found to be statistically significant in 23 parameters. In contrast to traditional methods the new method for analyzing human blood LPs is very simple and relatively quick. LP - lipoproteins; HDL - high density lipoprteins; LDL - low density lipoproteins; VLDL - very low density lipoproteins; CM - chylomicrons; TG - triglycerides; FC - free cholesterol; CE - cholesterol ester; PL - phospholipids; P - apolipoproteins; LPL - lipoproteinlipase; H-TGL - hepatic triglyceridlipase; DLP - dyslipoproteinemia; SAXS - small-angle X-ray scattering.

571/2523

Tytuł oryginału: 18 F-Fluorodeoxyglucose triple-headed coincidence detection imaging in oncology: preliminary results and comparison with dedicated PET.
Autorzy: Winter Frederic De, Wiele Christophe Van de, Vandenberghe Stefaan, Vandenbossche Bieke, D'Asseler Yves, Huglo Damien, Dierckx Rudi
Źródło: Med. Sci. Monitor 2002: 8 (6) s.MT89-MT94, il., tab., bibliogr. 2 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Dual head coincidence (DHC) imaging has been proposed as a valuable and cheaper alternative to oncological PET. The increased sensitivity of the recently-developed triple-headed gamma camera (THC) optimized for coincidence detection has not been either validated or compared to the sensitivity of a dedicated PET system in a clinical study. Differences in contrast-to-noise-ratio (CNR) between dedicated FDG PET, DHC and THC imaging were assessed using a hot sphere phantom. Twenty-two oncological patients were prospectively studied with consecutive whole body FDG PET adn FDG THC imaging. The images were visually read by 2 experienced nuclear medicine specialists. The diagnostic sensitivity of FDG THC imaging was assessed using FDG PET as the imaging gold standard. Lesion size was determined using computed tomography or magnetic resonance imaging. The mean gain in CNR for THC as compared to DHC imaging was 35 p.c. (10 - 56 p.c.). Of 63 lesions, 58 (92 p.c.) on FDG PET were also detected wtih THC imaging. Considering only lesions smaller than 15 mm, the relative sensitivity was 86 p.c. (24.29). All lesions aboe 15 mm (n = 34) were detected using THC imaging. The first clinical results for THC imging are promising. The overall relative sensitivity in this limited series was 92 p.c. Only a few lesions smaller than 15 mm were missed.

572/2523

Tytuł oryginału: The application of the Mancini technique as a diagnostic test in the CSF of tuberculous meningitis patients.
Autorzy: Kashyap Rajpal S., Agarwal Neha P., Chandak Nitin H., Taori Girdhar M., Biswas Saibal K., Purohit Hemant J., Daginawala Hatim F.
Źródło: Med. Sci. Monitor 2002: 8 (6) s.MT95-MT98, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Tuberculous meningitis (TBM) is still a serious cause of morbidity and mortality in developing nations, and the timing of treatment is the most crucial factor affecting the ultimate outcome. To establish a rapid diagnosis, we used Single Radial Immuno-Diffusion (SRID) to detect circulating mycobacterium antigen in the CSF of patients with clinically suspected TBM. Single radial immunodiffusion is the simplest of all immunotechniques for the quantitative determination of antigen or antibody. CSF was collected by standard lumbar puncture. Antiserum was raised against CSF from clinically suspected TBM patients by standard Immunization procedures. The developed protocol was tested with 73 CSF samples collected over a period of one year. The assay gave 94 p.c. sensitivity for the detection of mycobacterium antigen in the CSF of patients with clinically suspected TBM. This study suggests that single radial immunodiffusion is useful for quantitative as well as qualitative determination of mycobacterium antigen. The developed technique is of potential value in the laboratory diagnosis of TBM.

573/2523

Tytuł oryginału: Efficiency of CT-angiography in the diagnosis of intracranial aneurysms.
Autorzy: Sąsiadek Marek, Kowalewski Krzysztof, Turek Tomasz, Hendrich Barbara, Podkowa Jacek, Maksymowicz Hanna
Źródło: Med. Sci. Monitor 2002: 8 (6) s.MT99-MT109, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: CT-angiography is a non-invasive method, alternative to cerebral angiography in the diagnosis of intracranial aneurysms. The aim of the study was the assessment of the efficiency of CT-angiography in a large material, which has been mostly verified with angiography ad/or surgery. CT-angiography was performed in 196 patients with intracranial bleeding. Seventy-three (73) patients underwent also cerebral angiography (CA), 121 were operated on, 74 of them solely on the basis of CT-angiography. A spiral CT scanning (2 mm slices, 1-1.5 pitch, 1 mm-gap reconstruction) combined with an injection of 120 mL of contrast medium (5 mL/sec) was used. Subsequently three-dimensional MIP reconstructions were received, supplemented in some cases with SSD, VRT and MPR. CT-angiography revealed 128 aneurysms in 106 patients, located mostly in anterior communicating, middle cerebral and internal carotid arteries. The size of most aneurysms was 6 - 10 mm, but nearly one-third of them did not exceed 5 mm. In the group of patients who underwent CA, there was only 1 false negative CT-angiography result, while in 7 patients CT- angiography allowed for the diagnosis of small aneurysms which were not clearly visible on CA. CT-anioggraphy was superior to CA in assessment of aneurysm morphology by 3 independent radiologists in 17 out of 22 cases. CT-angiography findings were confirmed during surgery in all 74 patients. CT-angiography is highly effective in the diagnosis of intracranial aneurysms and the evaluation of their morphology. In our opinion, in most cases, CT-angiography is precise enough to plan a surgical or endovascular procedure.

574/2523

Tytuł oryginału: MR in neurological syndromes of connective tissue diseases.
Autorzy: Walecki Jerzy, Sierakowski Stanisław, Lewszuk Andrzej, Sulik Agnieszka, Tarasów Eugeniusz, Łebkowska Urszula
Źródło: Med. Sci. Monitor 2002: 8 (6) s.MT105-MT111, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Neurological complications in the course of SLE are mostly associated with vascular chagnes. An important role in their pathogenesis is played by immune mechanisms. The aim of the study was to determine the value of modern imging techniques, with special refrence to selected MR sequences (FLAIR, DWI) in the diagnosis of cerebral changes in patients with neurological symptoms of SLE. Fifty patients with neurological symptoms of SLE underwent CT and MR of CNS in routine sequences. In 12 cases EPI DWI sequences were also performed. Serum levels of antinuclear, anti-native DNA and antiphospholipid antibodies were also determined. The changes in CNS were detected in 48 patients in MR and in 42 patients in CT. Focal changes were observed in 29 cases, while atrophic chagnes were seen in the majority of subjects. In 10 cases, DWI showed changes typical for acute stroke. The extend and advancement of changes in CT and MR correlated with the severity of neurological symptoms; there was also a correlation between the changes and elevated levels of antiphosphlipid antibodies. FLAIR was useful in the detection of gliosis and cortical scar, while DWI enabled us to detect acute ischaemic foci and to distinguish them from coexistent scars. MRI with FLAIR and DWI sequences enhances the sensivity and specifity of neuroimaging techniques in the diagnosis of neropsychiatric disease in SLE. There is a correlation between elevated titre of antiphospholipid antibodies and the presence of vascular changes in patients with neurological manifestations of SLE.

575/2523

Tytuł oryginału: Stress-related diseases - a potential role for nitric oxide.
Autorzy: Esch Tobias, Stefano George B., Fricchione Gregory L., Benson Herbert
Źródło: Med. Sci. Monitor 2002: 8 (6) s.RA103-RA118, tab., bibliogr. 195 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Nitric oxide (NO) is involved in stress physiology and stress-related disease processes. Like stress, NO seems to be capable of principally exerting either beneficial or deleterious effects. The actual distinction depends on a multitude of factors. Moreover, NO conteracts norepinephrine (NE) activity and sympathetic responsivity. Thus, NO and the stress (patho)physiology are closely connected ad molecular mechanisms or pathways may be shared under certain conditions. NO is involved in immunological, cardiovascular, and neurodegenerative diseases/mental disorders. It represents a 'double-edged sword', since small quantities produced by constitutive enzymes may predominantly mediate physiological effects, whereas the expression of inducible NO synthases may lead to larger quantities of NO, a situation that may be associated with cytotoxic and detrimental effect of NO. The key step for normally useful physiological mechanisms beacoming pathophysiological may be represented by the loss of balance, the loss of control over the different pathways induced. A failure to terminate or shift originally protective mechanisms may lead to a vicious cycle of disease-supporting pathophysiological pathways. Profound connections between stress and various disease processe exist. Thereby, common pathophysiological pathways in stress-related diseases have been described, ant they involve stress hormone (cortisol, NE) and, in particular, NO activity. Thus, NO has detrimental capacities. However, NO not only exerts deleterious but also strongly ameliorating effects. The balance between both properties is crucial. Yet, nitric oxide involvement in stress-related disease represents a common pathway, with various pathophysiogical analogies, that may be accessible for strategies using stress mangement and relaxation response techniques.

576/2523

Tytuł oryginału: Hydrolysis of cortex peptidoglycan during bacterial spore germination.
Autorzy: Makino Shio, Moriyama Ryuichi
Źródło: Med. Sci. Monitor 2002: 8 (6) s.RA119-RA127, il., tab., bibliogr. 58 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Despite the most extreme dormancy and resistance properties among living systems, bacterial endospores retain an alert sensory mechanism to respond to the germinants and initiate germination. Although the molecular mechanism of the germiantion process is not completely described, current progress in the studies on the enzymes involved in the process gave us a some what clearer picture of the process of spore peptidoglycan (cortex) hydrolysis, a major biochemical event in germination. Germiantion-specific cortex-lytic enzymes require muramic acid ë-lactam in their substrates. At least two types of enzymes are involved in the germination process: a spore cortex-lyticenzyme (SCLE) and a cortical fragment-lytic enzyme (CFLE). Except for their peptidoglycan-binding regions, the primary structures of SCLE and CFLE vary according species. Both enzymes differ in their hydrolytic bond-specificities and recognition of the substrates morphology. SCLE appears to initiat germination by uncrosslinking the intract cortex, and the CFLE further degrades the polysaccharide moiety of the SCLE-modified cortex. In vivo CFLE activity is likely regulated by its requirement for partially uncrosslinked cortex, while SCLE requires activation process. Clostridium perfringens SCLE is activated by a germination-specific serine protease during germination, but the activation mechanism of SCLE in Bacillus species is unknown. Cortex-lytic enzymes are expressed at the early stage of sporulation but the compartment of expression depends on proteins. However, all enzymes are located outside the cortex layer in dormant spores, suggesting that the hydrolysis process initiates at the exterior side of the cortex. The assembly of the germination apparatus is also discussed.

577/2523

Tytuł oryginału: Wireless capsule endoscopy - beyond the frontiers of flexible gastrointestinal endoscopy.
Autorzy: Rabenstein Thomas, Krauss Norbert, Hahn Eckhart G., Konturek Peter
Źródło: Med. Sci. Monitor 2002: 8 (6) s.RA128-RA132, il., bibliogr. 32 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

psy

Streszczenie angielskie: Wireless capsule endoscopy is currently the outstanding technical innovation in diagnostic gastrointestinal endoscopy. Especially for small bowel diseases this new technique offers several potential advantages compared to traditional diagnostic tools. Capsule endoscopy is a painless procedure that can be performed as an ambulatory endoscopic examination. First experimental studies proved the good tolerance of the capsule endoscopy ad the possibility of a complete visual investigation of the small bowel. Clinical studies demonstrated possible fields of application: Obscure chronic or intermittent gastrointestinal blood loss and inflammatory bowel disease. The major risk of the procedure - intestinal obstruction by the capsule - may hinder its use in the diagnosis of polyps or tumors in the small bowel. In the next years it will be exciting to see whether capsule endoscopy will finally reach the clinical significance we expect at the moment. Possibly, the diagnostic algorithm for obscure gastrointestinal bleeding has to be changed in the future due to the abandonment of less effective procedures.

578/2523

Tytuł oryginału: Ultrastructural observations of intermediate cells in chronic pancreatitis.
Autorzy: Szynaka Beata, Zimnoch Lech, Puchalski Zbigniew
Źródło: Hepatogastroenterology 2002: 49 (46) s.1120-1123, il., bibliogr. 28 poz.
Sygnatura GBL: 304,355

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background/Aims: The intermediate cells exibiting both exo- and endocrine features occur in small numbers in the normal and pathologic pancreas of many animal species. In human pathology their presence has ben reported in children with hyperinsulinemia, in hypoglycemia, and chronic hypergastrinemia. Methodology: Fragments of the pancreas collected from 27 patients operated on for chronic pancreatitis of various intensity fibrosis (1ř - IVř) was subjected to ultrastructural analysis. Results: All chronic pancreatitis cases revealed the presence of intermediate cells that were found outside Langerhans islets, separately or in small groups. They were more common in IIIř and IVř chronic pancreatitis. All forms of chronic pancreatitis with Iř - IVř fibrosis showed, apart from undamage cells, numerous intermediate cells with features of destruction. Some intermediate cells contained fibrilate cytoplasm inclusion bodies. Intermediate cells took part in the formation of acini and were also found among ductural cells. Conclusions: The presence of intermediate cells both within the acinar texture and among proliferating ductular cells conforms the common origin of these cellular elements of the pancreas. In chronic pancreatitis intermediate cells appear during pancreatic texture regeneration, being rather abnormal cell forms produced in this process than an intermediate stage in precursor cell diferentiation.

579/2523

Tytuł oryginału: Mast cells and pancreatic stellate cells in chronic pancreatitis with differently intensified fibrosis.
Autorzy: Zimnoch Lech, Szynaka Beata, Puchalski Zbigniew
Źródło: Hepatogastroenterology 2002: 49 (46) s.1135-1138, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 304,355

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background/Aims: The pathogenesis of pancreatic fibrosis is unknown. Pathogenic analyses take into account the effect of oxidant stress and the increase in free radicals leading to degranulation of mast cells, which may cause inflammation and activate fibrosis. Activated pancreatic stellate cells produce abnormal components of intercellular substance and are responsible for pancreatic fibrogenesis. The aim of the study was to determine the correlation between the presence and state of mast cells and pancreatic stellate cells activation in chronic pancreatitis with varied intensity fibrosis. Methodology: We studied 27 patients with chronic pancreatitis of varied fibrosis intensity. Immunohistochemical reactions for mast cells (anti-human mast cell tryptase) and ultrastructural analyses of mast cells and pancreatic stellate cells were performed. The number of degranulated mast in the ultrastructural picture and the number of pancreatic stellate cells stained positive for vimentin and ŕ-smooth muscle actin were counted. Results: A significant increase was revealed in the number of degranulated mast cells - to 35.6 p.c. in chronic pancreatitis Iř fibrosis, 68.3 p.c. in IIř, 75.1 p.c. in IIIř and IVř (control 10.2 p.c.) and a parallel increase in the number of activated pancreatic stellate cells (stained positive for ŕ-smooth muscle actin) to 328 ń 29/mmý in Iř fibrosis, to 978 ń 67/mmý in IIř and 2355 ń 331/mmý in IIIř and IVř (control 38.8 ń 9/mmý). Conclusions: The increase in the number of activated pancreatic stellate cells parallel to the increase in degranulated...

580/2523

Tytuł oryginału: Plasmin/alpha2-antiplasmin complexes (PAP) in lung cancer patients: effects of radiotherapy.
Autorzy: Uszyński Mieczysław, Ziółkowska Ewa, Żekanowska Ewa, Uszyński Waldemar
Źródło: Thromb. Haemost. 2002: 88 (1) s.169-170, il., bibliogr. 2 poz.
Sygnatura GBL: 304,333

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

581/2523

Tytuł oryginału: The inhibitory effect of the fungicides captan and captafol on eukaryotic topoisomerases in vitro and lack of recombinagenic activity in the wing spot test of Drosophila melanogaster.
Autorzy: Rahden-Staroń Iwonna
Źródło: Mutat. Res 2002: 518 (1/2) s.205-213, il., tab., bibliogr. 53 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In studies on the mechanisms of mutagenic and carcinogenic action of captan and captafol-related chloroalkylthiocarboximide fungicides, two effects were tested: (i) the effect of both compounds on the activity of eukaryotic topoisomerase I and II in vitro, and (ii) their mutagenic and recombinagenic activity in the somatic mutation and recombination test (SMART) in wing cells of Drosophila melanogaster. Only captofal inhibited the activity of topoisomerase I (10-20 p.c. inhibition of activity in the range of 10-100 ćM). In contrast, both chemical decreased the activity of topoisomerase II already at 1 ćM concentration (50 and 20 p.c. inhibition of activity by captafol and captan, respectively). Genotoxicity was tested in vivo by administrating both compounds by acute (3 h) and chronic feeding (48 h) of 3-day-old exposure concentration range. Both chemical were inconclusive for large single spots, as well as for twin spots. In chronic treatment, captan showed positive results only for small single and total spots at 2.5 and 5 mM concentrations. Captafol gave inconclusive results over all concentrations tested. The results of the acute treatment experiments which have been performed at very high doses (50 p.c. toxicity at higher doses) indicate very weak overall mutagenic activity of both test fungicides.

582/2523

Tytuł oryginału: Urinary tract infections caused by endemic multi-resistant Enterobacter cloacae in a dialysis and transplantation unit.
Autorzy: Kamińska W., Patzer J., Dzierżanowska D.
Źródło: J. Hosp. Infect. 2002: 51 (3) s.215-220, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 306,296

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: In the period 1994-1998, 42 multi-resistant Eneterobacter cloacae isolates responsible for urinary tract infections (UTIs) were obtained from children hospitalized in our dialysis and transplantation unit. E. cloacae isolates obtained between 1994 and 1996 were susceptible to aminoglycosides, carbapenems and fluoroquinolones, whereas E. cloacae isolates obtained between 1997 and 1998 were susceptible to carbapenems and fluoroquinolones, only. All isolates were characterized by use of the polymerase chain reaction-based random amplification of polymorphic DNA and pulsed-field gel electrophoresis. It was found that the majority of multiresistant E. cloacae isolates obtained during 1997-1998, and all isolates obtained during 1994-1996, belonged to predominant clones A or B. These strains were responsible for gastrointestinal tract colonization and UTI of renal transplant recipients for several years, and persisted as endemic E. cloacae strains in the dialysis and transplantation unit from 1994 to 1998.

583/2523

Tytuł oryginału: The relationship between gastric cancer cells circulating in the blood and microsatellite instability positive gastric carcinomas.
Autorzy: Czopek J., Bialas M., Rudzki Z., Zazula M., Pituch-Noworolska A., Zembala M., Popiela T., Kulig J., Kołodziejczyk P., Stachura J.
Źródło: Aliment. Pharmacol. Ther. 2002: 16 suppl. 2 s.128-136, il., tab., bibliogr. 31 poz. - 9 Międzynarodowe Sympozjum nt. gastroenterologii Shimoda 26-27.04. 2001
Sygnatura GBL: 306,346

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bacground: Cancers characterized by microsatellite instability may be biologically different from their counterparts with stable microsatellite sequences. Circulating cancers cell present in blood prior to surgery may constitute an adverse prognostic finding. Aim: To correlate these two phenomena with morphological features and survival in advanced gastric cancer. Methods: We examined 76 cases of resected sporadic, advanced gastric cancer by means of routine morphology and a panel of microsatellite markers. Sixty-six cases were screened for presence of cancer cells circulating in blood prior to the surgery using combined morphological and immunocytochemical approach. Results: Twenry-one (27.6 p.c.) cases demonstrated microsatellite instability in at least one locus. Among them 11 (14.5 p.c. showed microsatellite instability in more than 30 p.c. (4/12) examined loci, and they were therefore designated as replication error positive (RER+). Circulating cancer cells were detected in 2/19 microsatellite instability and in 11/47 remaining cases (difference not significant). The survival of the microsatellite instability cases was significantly better. The presence of circulating cancer cells did not correlate with survival. Conclusion: It is possible that the microsatellite instability status, but not circulating cancer cells, constitutes a prognostic predictive factor in advanced gastric carcinoma. Confirmation of this hypothesis requires continuation of patient follow-up.

584/2523

Tytuł oryginału: Enrichment of normal progenitors in counter-flow centrifugal elutriation (CCE) fractions of fresh chronic myeloid leukemia leukapheresis products.
Autorzy: Dłubek Dorota, Dybko Jarosław, Wysoczańska Barbara, Laba Anna, Klimczak Aleksandra, Kryczek Ilona, Konopka Lech, Lange Andrzej
Źródło: Eur. J. Haematol. 2002: 68 (5) s.281-288, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 304,912

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Objectives: The aim of this study was to assess the suitability of a technique based on counter-flow centrifugal elutriation (CCE), which should allow one to enrich chronic myeloid leukemia (CML) patients' unstimalated native leukapheresis product (nLP) in CD34+ HLADR cells and BCR-ABL negative cells. Methods: Six newly diagnosed CML patients were subjected to leukapheresis, and the products were subfractionated with the use of CCE. nLP and all fractions were studied for the presence of CD34+ cells and a proportion of BCR-ABL fluorescence in situ hybridization (FISH)+ cells. Results: CCE fractions with a high flow rate contained the highest proportion of CD34+ cells [mean (SEM) 6.89 p.c. (3.88)]. However, CD34+ cells present in low-rate CCE fractions showed a higher proportion of HLADR- [49.6 p.c. (13.5 in 70 mL min -1) and 21.5 p.c. (11.6 in 110 mL min-1)] than those in 170 mL min-1 [3.2 p.c.(2.5)] and 'rotor off'[3.4 p.c. (1.9)]. This was associated with lower proportions of BCR-ABL FISH+ [8.1 p.c. (4.8) and 1.9 (1.7)] and smaller BCR-ABL to ABL transcript ratios [0.58 (17) and 0.26 (0.08) in 70 and 110 mL min-1] fractions as compared to 140 and 170 mL min-1 fractions [21.6 p.c. (5.2) and 31.6 p.c. (15.3) for BCR-ABL FISH+ cells and 0.75 (0.16) and 0.90 (0.24) for BCR-ABL/ABL]. Fractions with the lowest proportions of BCR-ABL-positive cells and the lowest BCR-ABL/ABL transcript ratios (110 mL min-1) contained from 1.3 x 10**6 to 82.7 x 10**6 (median: 3.97 x 10**6) CD34+ cells. Conclusions: In the present study we have shown that CCE may be used effectively to obtain nLP fractions enriched in normal hematopoietic progrenitors.

585/2523

Tytuł oryginału: Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets.
Autorzy: Majka Marcin, Ratajczak Janina, Villaire Gaston, Kubiczek Krystyna, Marquez Leah A., Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
Źródło: Exp. Hematol. 2002: 30 (7) s.751-760, il., tab., bibliogr. 51 poz.
Sygnatura GBL: 305,749

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: The development of megakaryocytes is regulated by thrombopoietin (TPO), which binds to the c-mpl receptor, and by several other cytokines such as interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), cilliary neurotropic factor (CNTF), and oncostatin (OSM), which bind to gp130 protein-coupled receptors. We attempted to identify signal transduction pathways activated by these factors in normal human megakaryocytes. Materials and methods: To better understand the role of these factors in normal human megakaryopoiesis we studied their effect on 1) purified human bone marrow-derived CD34+ cells, 2) human ŕIIbá3+ cells (shown by immunophenotypical and morphological criteria to be megakaryoblasts), which had been expanded ex vivo from CD34+ cells in chemically defined artificial serum, and 3) gel-filtered human peripheral blood platelets. Further, in an attempt to correlate the influence of these factors on cell proliferation and survival activation of signal transduction pathways, we evaluated their effect on the phosphorylation of MAPK p42/44 and activation of PI-3K-AKT and JAK-STAT proteins in these various cell types. Results: Using serum-free liquid cultures, we found that only TPO and IL-6 protected CD34+ cells and megakaryocytes from undergoing apoptosis (decrease in annexin-V binding, PARP cleavage, and activation of caspase-3). Moreover, only TPO when used alone and IL-6 only when used in combination with TPO, stimulated the growth of human colony-forming unitmegakarocytes (CFU-Meg) in semisolid serum-free medium...

586/2523

Tytuł oryginału: Synthesis, DNA-binding activity and cytotoxicity of carbamate derivatives of Hoechst 33258 in breast cancer MCF-7 cells.
Autorzy: Bielawski Krzysztof, Bielawska Anna, Wołczyński Sławomir
Źródło: Biol. Pharm. Bull. 2002: 25 (7) s.916-919, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 313,043

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: A series of carbamate derivatives of Hoechst 33258 was prepared as potential anticancer agents. These new compounds (1-4) were readily prepared in good yields by addition of chloroethyl, bromoethyl, chloropropyl or 4-(chloromethyl)phenyl isocyanates to Hoechst 33258. Their cytotoxic activity was evaluated on human breast cancer MCF-7. Compounds 1-4 were more cytotoxic than Hoechst 33258. In particular derivatives 4, the most active of the series, is up to 3 times more potent than Hoechst 33258. The DNA-binding ability of these compounds was evaluated by an ultrafiltration method using calf thymus DNA. These data show that in broad terms the cytotoxic potency of 1-4 in cultured breast cancer MCF-7 cells incerases, in accord with their increases in DNA affinity, as shown by the binding constant values.

587/2523

Tytuł oryginału: The effect of ascorbic acid and ethanol on the level of thiobarbituric acid reactive substances (TBARS) in selected tissues of albino BALB/c mice.
Autorzy: Woźniak Alina, Woźniak Bartosz, Drewa Gerard, Kasprzak Heliodor Adam
Źródło: Biol. Pharm. Bull. 2002: 25 (7) s.943-944, tab., bibliogr. 14 poz.
Sygnatura GBL: 313,043

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The aim of the paper was to study the effect of ascorbic acid (vitamin C) and ethanol on the level of thiobarbituric acid reactive substances (TBARS) in the brain, lungs and blood serum of inbred albino BALB/c mice. Sixty male mice divided into 4 groups were used for the study; the mice in each group were injected intraperitoneally (.p.): Group 1 - control, saline; Group 2 - H202 and FeSO4; Group 3 - ascorbic acid (AA); Group 4 - AA and ethanol. The level of TBARS in the investigated tissues of the mice in all of the groups was statistically significant higher than than in the control group. The highest content of TBARS in blood serum, brain, liver and lung tissue was observed in mice which were given ascorbic acid and ethanol.

588/2523

Tytuł oryginału: Impaired DNA damage-induced nuclear Rad51 foci formation uniquely characterizes Fanconi anemia group D1.
Autorzy: Godthelp Barbara C., Artwert Fr, Joenje Hans, Zdzienicka Małgorzta Z.
Źródło: Oncogene 2002: 21 (32) s.5002-5005, il., tab., bibliogr. [29] poz.
Sygnatura GBL: 312,857

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Fanconi anemia is a hereditary cancer susceptibility disorder chracterized at the cellular level by spontaneous chromosomal instability and specific hypersensitivity to DNA cross-linking agents such as mitomycin C. This phenotype suggests a possible role for the Fanconi anemia proteins in the repair of DNA lesions induced by these agents, but the molecular mechanism underlying the defect in this disorder has not yet been identified. Here, we show that amongst eight so far identified complementation groups of Fanconi anemia, only fibroblasts derived from group D1 are defective in the formation of nuclear Rad51 foci after X-ray irradiation or mitomycin C treatment. This indicates that the FANCD1 gene product is uniquely involved in the assembly and/or stabilization of the Rad51 complex. Since DNA dmage-induced Rad51 nuclear foci are thought to reflect of DNA double-strand breaks by homologous recombination, our results suggest that FANCD1 is likely to be involved in homologous recombination-dependent repair.

589/2523

Tytuł oryginału: Endothelium-dependent production and liberation of kynurenic acid by rat aortic rings exposed to L-kynurenine.
Autorzy: Stążka Janusz, Luchowski Piotr, Wielosz Marian, Kleinrok Zdzisław, Urbańska Ewa M.
Źródło: Eur. J. Pharmacol. 2002: 448 (2/3) s.133-137, il., bibliogr. [19] poz.
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Rat aortic slices produced and liberated the endogenous antagonist of glutamate receptors, kynurenic acid, upon exposure to L-kynurenine. Endothelium-denuded slices did not synthesize any measurable amount of kynurenic acid, indicating its endothelial origin. Aortic kynurenic acid production was diminished by modification of the ionic milieu, hypoxia and hypoglycemia, as well as by L-glutamate and L-aspartate, endogenous glutamate receptor agonists, and aminooxyacetic acid, a non-selective inhibitor of aminotransferase and mitochondrial respiration. These data pave the way for future research aimed to clarify to the role of kynurenic acid in the physiology and pathology of the endothelium and vasculature.

590/2523

Tytuł oryginału: Effect of imipramine on brain D-1 and 5-HT-2A receptors in a chronic unpredictable stress model in rats.
Autorzy: Ossowska Grażyna, Nowak Gabriel, Klenk-Majewska Bożena, Danilczuk Zofia, Żebrowska-Łupina Iwona
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.89-93, tab., bibliogr. 40 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Chronic unpredictable stress (CUS) model of depression is one od the well validated animal models of depression. In this paper, we report the results of investigations into dopaminergic D-1 and serotonergic 5-HT-2A receptors in the brain of rats subjected to CUS procedure and treated chronically with imipramine. We have examined the dopaminergic D-1 ([3H-SCH 23390) in the limbic area and serotonergic 5-HT-2A ([3H-ketanserin) receptors in the cerebral cortex by a saturation radioligand binding method in rats subjected to CUS paradigm, imipramine, both CUS and imipramine and control animals. CUS procedure resulted in a significant 36 p.c. increase in the D-1 receptor density in the limbic system, which was attenuated by chronic imipramine treatment. Also a 21 p.c. increase in the density of 5-HT-2A receptors in the cerebral cortex induced by CUS was reduced by chronic imipramine treatment. The present data indicate that the increases in the density of brain D-1 and 5-HT-2A receptors of rats subjected to CUS, which are "normalized" by imipramine, might be involved in the pathophysiology of :animal depression" (and, thus, in pathophysiology of human depression) and in the mechanism of antidepressant tharapy.

591/2523

Tytuł oryginału: Behavioral effects of the selective blockade of metabotropic glutamate receptor subtype 5 in experimental hypoxia.
Autorzy: Nadlewska Agnieszka, Car Halina, Wiśniewska Róża J., Wiśniewski Konstanty
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.95-102, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The behavioral activity of 2-methyl-6-(phenylethynyl)-pyridine (MPEP) a selective antagonist of metabotropic glutamate receptor subtype 5 (mGluR5), was assessed in control groups of rats and in rats that underwent short-term hypoxia. MPEP applied intravenously (iv) at the dose of 1 mg/kg was tested using the open field test, the passive avoidance test and the elevated "plus" mzae test. MPEP significantly increased the number of crossings, rearings and bar approaches in the open field test. It improved the consolidation and retrieval in the passive avoidance situation and did not produce any significant effects in control rats in the elevated "plus" maze test. Hypoxia (2 p.c. O2, 98 p.c. N2) significantly inhibited locomotor and exploratory activity of rats and also impaired consolidation and retrieval processes, but it did not evoke any changes in the elevated "plus" maze test. MPEP in hypoxia-exposed groups of rats inhibited locomotor and exploratory activity in comparison with MPEP-treated control group. Hypoxia significantly diminished beneficial effect of MPEP on consolidation. In spite of experimental conditions of hypoxia, the effect of MPEP on retrieval was preserved. MPEP used before hypoxia did not significantly influence all parameters of the elevated "plus" maze test. In conclusion, MPEP had beneficial effect on retrieval in hypoxia.

592/2523

Tytuł oryginału: Effects of tamoxifen, mifepristone and cyproterone on the electroconvulsive threshold and pentetrazole-induced convulsions in mice.
Autorzy: Borowicz Kinga K., Łuszczki Jarogniew, Matuszek Mariusz, Kleinrok Zdzisław, Czuczwar Stanisław J.
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.103-109, tab., bibliogr. 40 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The aim of this study was to evaluate the efficacy of three antihormones, tamoxifen (TXF, an antiestrogen), mifepristone (MIF, an antiprogesterone) and cyproterone (CYP, an antiandrogen) in two major models of experimental epilepsy, electriccally and pentetrazole (PTZ)-evoked seizures in mice. TXF (20-50 mg/kg) significantly raised the threshold for electroconvulsions in female mice, whereas CYP was active in male mice. Similar effects were observed in castrated mice. Different data were obtained in sexually immature animals since both TXF and CYP exerted anticonvulsive effects in animals of both genders. MIF (5-50 mg/kg) remained without effect on electrically evoked seizures in mice. The anticonvulsive action of TXF was reversed by aminophylline, bicuculline, kainic acid and N-methyl-D-aspartic acid, but not estradiol or strychnine. The protective action of CYP was reversed by aminophylline and bicuculline, but not by testesterone, kainic acid, N-methyl-D-aspartic acid or strychnice. All three antihormones were inffective against PTZ-induced convulsions in mice. Our results suggest that the action of TXF and CYP might be indirectly associated with the respective hormonal receptor-mediated events, but the nature of this dependence is unclear and further investigations are needed to elucidate this phenomenon.

593/2523

Tytuł oryginału: Lack of interaction between the behavioral effects of ketamine and benzodiazepines in mice.
Autorzy: Fidecka Sylwia, Pirogowicz Ewa
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.111-117, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The effect of co-administration of ketamine at the sub-effective dose with diazepam, chlordiazepoxide and clonazepam on their antinociceptive and protective efficacy against pentetrazole-induced seizures were studied in mice. Ketamine alone produces dose-dependent antinociception manifested as reduction in the number of writhing episodes evoked by acetic acid. In the writhing test, the antinociceptive effects of the threshold doses of diazepam, chlordiazepoxide or clonazepam were not changed by ketamine, whereas that of morphine was intensified by ketamine. In the hot plate test, slight antinonciceptive effects of the threshold dose of diazepam, but not that of chlordizepoxide (except the results at 120 min of observation), were significantly intensified by ketamine vs ketamine alone. Ketamine alone was able to protect mice, in the dose-related manner, against penetrazole-induced seizures. The anticonvulsant effects of the threshold doses of diazepam, chlordizepoxide and clonazepam were not changed by ketamine. These findings indicate that co-administration of ketamine (at the sub-effective dose) with diazepam, chlordiazepoxide and clonazepam (at non-effective) resulted in an intensification of neither antinociceptive nor protective effect against pentetrazole-induced seizures in mice. These data seem to indicate the lack of interaction between ketamine and benzodiazepines with respect to their antinociceptive and anticonvulsant efficacy.

594/2523

Tytuł oryginału: Adenosine receptor ligands and cocaine in conditioned place preference (CPP) test in rats.
Autorzy: Poleszak Ewa, Malec Danuta
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.119-126, il., bibliogr. 38 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The influence of adenosine receptor agonists and antagonists on cocaine-induced conditioned place preference (CPP) was examined in male Wistar rats. Adenosine receptor agonists, when given alone, induced place preference in some dose ranges, and it seems that adenosine A1 and A2 receptors might be involved in this reaction. All adenosine receptor agonists: 2-p-(2-corboxyethyl)phenethylamino-5'-N-ethylcarboxamidoadenosine (CGS 21680), A2A receptor agonist, N6-cyclopentyladenosione (CPA), A1 receptor agonist, and 5'-N-ethylcarboxamidoadenosine (NECA), A2/A1 receptor agonist did not prevent the acquisition of cocaine-induced CPP but, when administered at the lower doses, they reduced the expression of cocaine action in CPP test. Selective adenosine A1 receptor antagonist, 8-cyclopentyltheophylline (CPT), A2 receptor antagonists, 3,7-dimethyl-1-propargylxanthine, DMPX, and caffeine (non-selective A1/A2 receptor antagonist) markedly and significantly decreased the expression of CPP induced by cocaine, and caffeine (20 mg/kg) decreased also the acquisition of this reaction. Our results suggest the involvement of adenosine A1 and A2 receptors in rewarding prperties of cocaine measured in CPP test.

595/2523

Tytuł oryginału: Effect of subchronic ethanol treatment on plasma and cerebrospinal fluid leptin levels in rats selectively bred for high and low alcohol preference.
Autorzy: Mikołajczak Przemysław, Okulicz-Kozaryn Irena, Kamińska Ewa, Wiktorowicz Krzysztof, Leśniewska Kinga, Dyr Wanda, Kostowski Wojciech
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.m27-132, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The effect of 5-week voluntary ethanol (EtOH) intake on plasma and cerebrospinal fluid (CSF) leptin levels was determined in adult male Warsaw high EtOH preferring (WHP) and low preferring (WLP) rats. EtOH treatment led to a decrease in leptin CSF concentration in WHP rats when compared to EtOH-naive WHP and control Wistar rats. On the contrary, in EtOH-treated WLP rats, both plasma and CSF leptin levels were increased in comparison with EtOH-naive animals. It can be concluded that EtOH treatment led to different response expressed especially by SCF leptin levels in WHP and WLP animals and it may be related to their genetic predisposition.

596/2523

Tytuł oryginału: Effects of porcine galanin, galanin(1-15)NH2 and its new analogues on glucose-induced insulin secretion.
Autorzy: Ruczyński Jarosław, Cybal Magdalena, Wójcikowski Czesław, Rekowski Piotr
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.133-141, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Porcine galanin (pGAL), its 15-amino-acid-residue fragment and five new analogues modified in positions 4, 6 or 14 were tested for their effects on glucose-induced insulin secretion from isolated rat pancreatic islets of Langerhans. In vitro insulin secretion was studied during static incubation. All peptides were tested at two concentrations: 100 nM and 1 ćM. The insulin level in the presence of 10 mM of glucose was a reference for all experiments. Our studies have shown that porcine galanin and its fragment GAL(1-15)NH2 at all concentrations tested inhibit glucose-induced insulin secretion. However, the modifications of the amino acod sequence of galanin caused changes in the interaction of GAL with its receptors, consequently yielding peptides that showed reverse activity as compared to pGAL or GAL(1-15)NH2. Finally, we have found three analogues: [Cit14]GAL(1-15)-NH2, [Hse6]GAL(1-15)NH2 and [Cle4]GAL(1-15)NH2, which were able to stimulate glucose-induced insulin secretion and also antagonized inhibitory effect of pGAL. Other two galanin analogues: [D-Leu4]GAL(1-15)NH2 and [des-Leu4]GAL(1-15)NH2 showed a rather weak agonistic activity. Our observations suggest that positions: 4, 6 and 14 in the amino acid sequence of galanin may play an important role in the high-affinity binding of GAL to its receptors and biological action in perfused rat pancreas.

597/2523

Tytuł oryginału: Cerivastatin modulates plasma paraoxonase/arylesterase activity and oxidant-antioxidant balance in the rat.
Autorzy: Bełtowski Jerzy, Wójcicka Grażyna, Mydlarczyk Mariusz, Jamroz Anna
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.143-150, il., bibliogr. 49 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Statins (3-hydroxy-3-methylglutarylcoenzyme A reductase inhibitors), apart from lowering plasma cholesterol, modulate other processes involved in atherogenesis. Paraoxonase (PON), contained in plasma high density lipoproteins, protects plasma lipoproteins from oxidative damage and is a potentially atheroprotective enzyme. We investigated the effect of cervastatin on oxidant-antioxidant balance and plasma PON activity. The adult male Wistar rats received cervistatin at a dose of 0.03 or 0.3 mg/kg/day for 3 weeks. Then, plasma concentration of lipid peroxidation products, total antioxidant capacity and PON activity were assayed. Plasma level of lipid peroxidation products was unchanged in low-dose group but decreased significantly in animals receiving high dose of cervivastatin. In this group, the concentration of malonyldialdehyde and 4-hydroxydialkenals was reduced by 46.6 p.c. whereas the level of lipid hydroperoxides was lowered by 59.3 p.c. Total plasma antioxidant capacity increased in low-dose group by 22.3 p.c. and in high-dose group by 27.2 p.c. PON activity toward paraoxon decreased by 16.1 p.c. and 11.6 p.c. in low- and high-dose groups, respectively. The activity toward phenyl acetate, which better corresponds wuith enzyme concentration, declined by 74.2 p.c. and 78.4 p.c. following lower and higher dose treatment, respectively. PON/arylesterase ratio raised in cervastatin-treated rats (low dose: +227.4 p.c., high dose: +328.2 p.c.).Cerivastatin had no effect on total plasma cholesterol but significantly decreased triglyceride level by 34.6 p.c...

598/2523

Tytuł oryginału: Influence of midazolam pharmacokinetic parameters of procainamide in rabbits.
Autorzy: Orszulak-Michalak Daria, Owczarek Jacek, Wiktorowska-Owczarek Anna K.
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.151-155, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: The majority of antiarrhytmic drugs have very narrow therapeutic range, and they may cause some side effects at doses used for curing cardiac arrhythmias. These drugs may enter different interactions. Procainamide also may interact with other drugs. Also some other drugs may change pharmacokinetics of procainamide, for example the iv anesthetics influence on pharmacokinetic parameters of procainamide. The aim of the study was to investigate the influence of midazolam on the plasma concentrations and pharmacokinetic parameters of procainamide in rabbits during two hours of obserwation. Procainamide was administered in rabbits at a dose of 13 mg/kg iv, and midazolam at 0.2 mg/kg iv. Procainamide levels were determined by immunofluorescence polarization method using ABBOTT reagents. Levels of procainamide were determined in the plasma at 5, 10, 15, 30, 45, 60, 90 and 120 min after the administration of procainamide. After administration of midazolam with procainamide, a decrease in plasma concentration of procainamide, together with its increased elimination, was observed.

599/2523

Tytuł oryginału: Interactions between human platelets and eosinophils are mediated by selectin-P.
Autorzy: Jawień Jacek, Chłopicki Stefan, Gryglewski Ryszard J.
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.157-160, tab., bibliogr. 15 poz.
Sygnatura GBL: 313,156

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Mechanisms and biochemical consequences of platelet-neutrophil interactions are well known. In contrast, platelet-eosinophil interactions remain largely unexplored. The aim of this study was to assess whether platelets adhere to eosinophils, and to analyze whether selectin-P would mediate that phenomenon. Eosinophils and platelets were obtained from peripheral blood of healthy volunteers. Eosinophils were isolated using magnetic cell separation method. Platelets were isolated and washed. A number of "rosettes" (an eosinophil with more than 5 adherent platelets) per 100 eosinophils were examined in the eosinophil-platelet suspension. Addition of thrombin stimulated formation of "rosettes". Monoclonal antibodies against selectin-P almost completely prevented thrombin-stimulated formation of "rosettes". In summary, intercellular interaction between platelets and eosinophils are mediated by selectin-P. This phenomenon may be of importance in asthma and other atopic diseases.

600/2523

Tytuł oryginału: Saliva as an alternative specimen for alcohol determination in the human body.
Autorzy: Gubała Wojciech, Zuba Dariusz
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.161-165, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 313,156

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Saliva, breath, and blood samples were collected from 49 volunteers and over 700 values of ethanol concentration were obtained. The profiles of time-dependent changes in saliva and expired air-breath ethanol concentration were similar. The average difference between the respective values determined in blood and saliva amounted to -0.031 ń 0.096 g/l, whereas the difference between the results for breath and saliva was -0.034 ń 0.080 g/l. These differences in ethanol concentrations do not exceed those which occur between blood and breath (0.003 ń 0.093 g/l). Introducing a correction value of 1.08, stemming from the varying water content in saliva and blood, results in a good agreement between the results for saliva and breath (0.005 ń 0.077 g/l). The headspace gas chromatgraphic method applied for ethanol determination in saliva is specific (resolution 1), shows good accuracy (recovery = 100.7 p.c.) and precision (SD = 0.0155 g/l). There is no matrix effect when water solutions are used for calibration instead of saliva.

601/2523

Tytuł oryginału: The Eleventh Days of Neuropsychopharmacology.
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.167-206, il., tab., bibliogr. [przy ref.] - 11 Dni Neuropsychofarmakologii Ustroń-Jaszowiec 27-29.05. 2002 - Część materiałów zamieszczono w formie streszcz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

602/2523

Tytuł oryginału: 5-HT1A receptors. Their role in anxiety and depression mechanism of activation.
Autorzy: Chilmonczyk Zdzisław
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.175-178, il., bibliogr. 38 poz. - 11 Dni Neuropsychofarmakologii Ustroń-Jaszowiec 27-29.05. 2002
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

603/2523

Tytuł oryginału: Serotonin and Parkinson's disease.
Autorzy: Kostrzewa Richard M., Brus Ryszard
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.178-179 - 11 Dni Neuropsychofarmakologii Ustroń-Jaszowiec 27-29.05. 2002
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

604/2523

Tytuł oryginału: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) as endogenous toxicants of CNS: some aspects of defense.
Autorzy: Metodiewa Diana, Kochman Agata
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.179-181, bibliogr. 17 poz. - 11 Dni Neuropsychofarmakologii Ustroń-Jaszowiec 27-29.05. 2002
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

605/2523

Tytuł oryginału: DT-diaphorase: a neuroprotective enzyme of dopaminergic systems.
Autorzy: Paris I., Martinez P., Arriagada C., Dagnino-Subiabre A., Montiel J., Caviedes P., Caviedes R., Mora S., Diaz-Veliz G., Cassel B., Olea-Azar C., Aboitiz F., Segura-Aguilar J.
Źródło: Pol. J. Pharmacol. 2002: 54 (2) s.181-183, bibliogr. 13 poz. - 11 Dni Neuropsychofarmakologii Ustroń-Jaszowiec 27-29.05. 2002
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

606/2523

Tytuł oryginału: Low-dose ritonavir moderately enhances nelfinavir exposure.
Autorzy: Kurowski Michael, Kaeser Benoite, Sawyer Anthony, Popescu Matei, Mrozikiewicz Alexander
Źródło: Clin. Pharmacol. Ther. 2002: 72 (2) s.123-132, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 311,916

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Bacground: The protease inhibitor ritonavir is increasingly administered at subtherapeutic doses in highly acyive antiretroviral treatment, to utilize its potential for drug interactions and to enhance the plasma concentrations of other concomitantly prescribed protease inhibitors. The addition of low doses of ritonavir to nelfinvir as investigated to describe the extent of pharmacokinetic interaction. Methods: In this randomized, open-label, one-sequence crossover study, nelfinavir 1250 mg a day was dosed for 17 days, followed by 14 days of nelfinvar 1250 mg twice a day plus low doses of ritonavir of either 100 mg or 200 mg orally. Twenty-four healthy volunteers were evaluated for pharmacokinetics of nelfinavir, its metabolite M8, and ritonavir. Plasma concentrations were measured up 12 hours after moring and evening dosing, respectively, on days 14 and 31. Results: Ritonavir increased the area under the plasma concentration-time curve (AUC) of nelfinvir by 20 p.c. (P = .024) and 39 p.c. (P = .001) after moring and evening administration, respectively. The AUC of nelfinavir metabolite M8 was increased by 74 p.c. and 86 p.c. after moring and evening dosing (P .001 for both). Conclusion: During ritonavir combination therapy a clear although minor drug effect on nelfinavir pharmacokinetics was demonstrated but no dose effect was shown.

607/2523

Tytuł oryginału: Jan Mikulicz-Radecki (1850-1905) - the creator of modern European surgery.
Autorzy: Gorecki Piotr, Gorecki Wojciech
Źródło: Dig. Surg. 2002: 19 (4) s.313-318, il., bibliogr. 26 poz.
Sygnatura GBL: 313,013

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Temat osobowy:

Mikulicz-Radecki

Jan 1850-1905

608/2523

Tytuł oryginału: Atherosclerosis
Tytuł angielski: Inverse relationship between total testosterone and anti-oxidized low density lipoprotein antibody levels in ageing males.
Autorzy: Barud Wojciech, Palusiński Robert, Bełtowski Jerzy, Wójcicka Grażyna
Źródło: Atherosclerosis 2002: 164 (2) s.283-288, il., tab., bibliogr. 49 poz.
Sygnatura GBL: 304,815

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Accumulating evidence indicates the involvement of sex hormones in atherogenesis. Endogenous testosterone is inversely related to the majority of risk factors for atherosclerosis and is known to be a potent immunomodulator. Recently, autoantibodies to oxidized LDL (anti-oxLDL Ab) were shown to predict carotid and coronary atherosclerosis. The aim of this study was to investigate the relationship between these antibodies and testosterone level in ageing males. The study group comprised 65 males over 50 years old (42 with coronary artery disease). Serum anti-oxLDL Ab titer was measured by enzyme-linked immunoassay and total serum testosterone by radioimmunoassay. A significant inverse correlation was found between serum anti-oxLDL Ab titer and testosterone concentration (r = -0.346, P = 0.0047). Alteration in serum anti-oxLDL Ab titers showed no correlation to classical cardiovascular risk factors, e.g. body mass index, waist/hip ratio, smoking, total cholesterol, triglicerides, HDL-cholesterol, LDL-cholesterol. In multiple regression analysis only testosterone level was independently associated with anti-oxLDL Ab. These data suggest that a fall of testosterone concentration in ageing men can influence either oxidative modification of LDL or the immune response to these lipoproteins which may be important in the pathogenesis of atherosclerosis.

609/2523

Tytuł oryginału: Azapeptides structurally based upon inhibitory sites of cystatins as potent and selective inhibitors of cysteine proteases.
Autorzy: Wieczerzak Ewa, Drabik Piotr, Łankiewicz Leszek, Ołdziej Stanisław, Grzonka Zbigniew, Abrahamson Magnus, Grubb Anders, Brmme Dieter
Źródło: J. Med. Chem. 2002: 45 (19) s.4202-4211, il., tab., bibliogr. 69 poz.
Sygnatura GBL: 312,133

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: A series of azapeptides as potential inhibitors of cysteine protease were synthesized. Their structures, based on the binding center of cystatins, contain an azaglycine residue (Agly) in place of the evolutionary conserved glycine residue in the N-terminal part of the enzyme binding region of cystatins. Incorporation of Agly should lead to deactivation of the acyl-enzyme complex formed against nucleophilic attack by water molecules in the final step of peptide bond hydrolysis. The majority of synthesized azapeptides shows high inhibitory potency toward the investigated cysteine proteases, papain, cathepsin B, and cathepsin K. One of them, Z-Arg-Leu-Val-Agly-Ile-Val-OMe (compound 17), which contains in its sequence the amino acid residues from the N-terminal binding segment as well as the hydrophobic residues from the first binding loop of human cystain C, proved to be a highly potent an selective inhibitor of cathepsin B. It inhibits cathepsin B with a Ki value of 0.088 nM. To investigate the influence of the structure of compound 17 for its inhibitory properties, we determined its conformation by means of NMR studies and theoretical calculations. The Z-Arg-Leu-Val-Agly fragment covalenty linked to Cys29 of cathepsin B, was also developed and modeled, in the catalytic pocket of the enzyme, through a molecular dynamics approach, to analyze ligand - protein interactions in detail. Analysis of the simulation trajectories generated using the AMBER force field provided us with atomic-level understanding of the conformational variability of this inhibitor, which is discussed in the context of other experimental and theoretical data.

610/2523

Tytuł oryginału: Global motion detection is impaired in cats deprived early of pattern vision.
Autorzy: Burnat Kalina, Vandenbussche Erik, Żernicki Bogusław
Źródło: Behav. Brain Res. 2002: 134 (1/2) s.59-65, il., bibliogr. 45 poz.
Sygnatura GBL: 312,662

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

koty

Streszczenie angielskie: We investigated global motion detection in binocularly deprived cats (BD cats) and control cats (C cats). The cats were trained in the two-choice free running apparatus for a food reward. The positive stimulus was a moving random-dot pattern with all dots moving in one direction, the negative stimulus was the same random-dot pattern but stationary. The BD cars were severly impaired in detection of global motion stimulus as compared with the C cats. In contrast, their level of performance in a simple relative motion detection task (one square) did not differ from that in the C cats. However, in more complex relative motion detection task (two squares) the performance of the BD cats was impaired. The deficit in the detection of global motion in BD cats may be due to impairments of their Y-pathway.

611/2523

Tytuł oryginału: Septal vasopressin induced preservation of social recognition in rats was abolished by pinealectomy.
Autorzy: Appenrodt Edgar, Juszczak Marlena, Schwarzberg Helmut
Źródło: Behav. Brain Res. 2002: 134 (1/2) s.67-73, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 312,662

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The role of intraseptal vasopressin (AVP) and the pineal gland in the modulation of social memory was investigated. For social recognition, male pinealectomised (Px) and sham-operated (SO) rats were confronted with juveniles for 4 min, and injected with either 200 pg AVP or 5 ng of its V1 receptor antagonist d(CH2)5Tyr(Me)AVP(AAVP) into the mediolateral septum. Re-exposure to the same and a different juvenile took place after 30 or 120 min. In SO rats, the social memory was facilitated after injection of AVP (120 min) and impaired after AAV application (30 min). In Px rats, however, neither AVP nor AAVP administration influenced the social memory. Comparison between SO and Px control groups treated with artificial cerebrospinal fluid did not reveal any differences as to social recognition responses. After subcutaneous administration of 250 ćg melatonin (substitution for pinealectomy) the social discrimination responses in Px rats after peptide application were influenced in a manner identical to that noted in SO animals. These finding suggest that the modulation of social memory by intraseptal AVP is dependent on an intact pineal function.

612/2523

Tytuł oryginału: Comparison of Multiplex PCR, and an immunochromatographic method sensitivity for the detection of Escherichia coli O157:H7 in minced beef.
Autorzy: Gryko Romuald, Sobieszczańska Beata M., Stopa Peter J., Bartoszcze Michał A.
Źródło: Acta Microbiol. Pol. 2002: 51 (2) s.121-129, il., tab., bibliogr. [30] poz.
Sygnatura GBL: 302,697

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In this study, the sensitivities of multiplex PCR and an immuno-chromatographic methods to detect Escherichia coli 0157:H7 in minced beef were compared. The detection of Escherichia coli 0157:H7 in minced beef inoculated with 1-100 cells of this bacterium was possible after enrichment of culture and subsequent analysis by either of two methods. Enrichment conditions were eight hours of incubation at 37řC or 42řC in a non-selective medium (Buffered Peptone Water). Multiplex PCR analysis was performed using three primer sets with analysis by gel electrophoresis. The QuixTM immuno-chromatographic assay which is a new kit being marketed by New Horizons Diagnostics, Columbia, MD, was used for immunological analysis of the enriched broths. The sensitivity of both tests was similar. The results depended on the concentration of the specific bacterium in the culture since the influence of the proportion of other bacteria to the E. coli 0157:H7 was not observed. The data suggests taht either method or used together, when coupled with an enrichment technique, could provide a rapid mean to detect the presence of this pathogen in minced meat samples.

613/2523

Tytuł oryginału: Protein Z in young survivors of ischemic stroke.
Autorzy: Łopaciuk Stanisław, Bykowska Ksenia, Kwieciński Hubert, Członkowska Anna, Kuczyńska-Zardzewiały Arletta
Źródło: Thromb. Haemost. 2002: 88 (3) s.536, il., bibliogr. 5 poz.
Sygnatura GBL: 304,333

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

614/2523

Tytuł oryginału: Health and social problems of addicted persons on methadone treatment program.
Tytuł polski: Problemy zdrowotne i społeczne osób uzależnionych objętych programem metadonowym.
Autorzy: Karakiewicz Beata, Kozielec Tadeusz, Durska Grażna, Stanaszek Roman
Źródło: Pol. Med. Rodz. 2002: 4 (3) s.365-369, il., tab., bibliogr. 13 poz., streszcz.
Sygnatura GBL: 313,549

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: W pracy przeanalizowano wybrane problemy zdrowotne i społeczne u osób uzależnionych od substancji psychoaktywnych. Badaniem objęto 70 osób - pacjentów oddziału detoksykacyjnego oraz programu metadonowego SPS ZOZ-u Psychiatrycznego "Zdroje" w Szczecinie. Z przeprowadzonych badń wynika, że mężczyźni częściej uzależniają się od substancji psychoaktywnych niż kobiety. Osoby uzależnione od narkotyków mają niski poziom wykształcenia i często są bezrobotne (bez prawa do zasiłku). U większości badanych osób w przebiegu uzależnienia wystąpiły dodatkowe problemy zdrowotne. Terapia substytucyjna programu metadonowego osób uzależnionych od opiatów w sposób znaczący wpływa na redukcję szkód zdrowotnych i społecznych narkomanii.

Streszczenie angielskie: The paper contains analysis of chosen social and health problems in psychoactive substance abusers in Szczecin, Poland. Seventy psychoactive substance dependent individuals were enrolled into the study (patients from the Detoxification Ward and patients participating in methadone program). The results of the study showed higher uncidence of psychoactive substance abuse in males than in females. Low educational level and unemployment were found in the studied population. Drug dependent individuals frequently had criminal record. The following additional health problems were found in psychoactive substance abusers: epilepsy, inflammatory skin disease, central nervous system injuries, HIV and hepatotropic viruses cerrier state. Methadone program allows reduction of health and social harms in opiate dependent patients.

615/2523

Tytuł oryginału: Activity of neuraminidase on the structure of rabbit kidney glomerular basement membranes; spin label study.
Tytuł polski: Ocena wpływu neuraminidazy na strukturę błony podstawnej kłębuszków nerkowych w modelu eksperymentalnym u królików za pomocą techniki rezonansu spinowego.
Autorzy: Sapilak Bartosz J., Steciwko Andrzej, Olsztyńska Sylwia, Komorowska Małgorzata
Źródło: Pol. Med. Rodz. 2002: 4 (3) s.371-375, il., bibliogr. 23 poz., streszcz.
Sygnatura GBL: 313,549

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie polskie: Cel badania: Jakkolwiek relatywnie dobrze poznano etiologię i patogenezę kłębuszkowego zapalenia nerek (k.z.n.), to nadal nie możemy poznać zachodzących w mikrostrukturze bariery filtracyjnej kłębuszków prowadzących do utraty selektywności filtracji i powstawania proteinurii. Dotychczas stowane techniki badawcze okazały się mało przydatne. W naszych badaniach podjęliśmy próbę zaadaptowania nowej techniki elektronowego rezonansu spinowego (ERS), by uzyskać istotne informacje o patomechanizmie k.z.n. Materiał i metody: Do badań użyliśmy królików obojga płci dobranych pod kątem dobrej kondycji zdrowotnej i wieku (1,5-2 lata). Zwierzętom podawaliśmy dożylnie przez kolejne 3 dni aktywną enzymatycznie neuraminidazę, zgodnie ze schematem wypracowanym w naszym laboratorium. Dla celów doświadczenia utworzono jedną grupę kontrolną i pięć grup badanych, różniących się dawką podawanej neuraminidazy. Po skrwawieniu zwierząt pobrano obie nerki, wypreparowano ich korę, z której na drodze obróbki mechanicznej uzyskano najpierw kłębuszki nerkowe, a następnie na drodze wielokrotnej ultrasonifikacji wolną od zanieczyszczeń tkankowych zawiesinę błon podstawnych kłębuszków nerkowych (BP). Roztwór BP znakowano jednym z dwóch znaczników spinowych: MSL (3-maleimido-1-oxyl-2,2,5,5,-tetramethylpyrrolidiny) lub JAA (jodoacetamid-1-oxyl-2,2,5,5,-tetramethylpyrrolidiny). Uzyskane widma otrzymano badając tak przygotowany materiał na spektrometrze Se/X-28. By ocenić ruchliwość znaczników spinowych podczas ich izotropowej rotacji w polu elektromagnetycznym, wyznaczano czas korelacji...

Streszczenie angielskie: Purpose of studies: an estimation of structural and three-dimensional changes in organization of kidney glomerular basement membrane components appearing in experimentally caused glomerulonephritis. We have used 1,5 - 2 years old rabbits. They were divided into a few groups, with different amount of intravenous injected active neuraminidase, and also separate control group. Glomerular basemet membranes from collected animals kidneys according to method developed by Spiro were prepared. Then obtained preparates were labelled by 3-maleimido-1-oxyl-2,2,5,5,-tetramethylpyrrolidine (MSL) and 4-(iodoacetamido)-1-oxyl-2,2,5,5,-tetramethylpyrrolidine (JAA) sulfhydryl specific spin labels. Measured ESR spectra of labelled membranes show significant changes of spin labels mobility and this effect dependents on neuraminidase concentration. In comparison with intact membranes neuraminidase action on membranes creates three different ESR signals what is connected with different protein fractions. The changes in structure of basement membranes caused by nauraminidase correspond with increasing glomellural wall permability.

616/2523

Tytuł oryginału: Fibrous-long spacing collagen in post-partum rat cervix uteri.
Autorzy: Mackiewicz Zygmunt, Stepaniuk Mirosława, Mackevicius Zigmuntas jr
Źródło: Acta Med. Lituan. 2002: 9 (2) s.126-129, il., bibliogr. 20 poz., santr.
Sygnatura GBL: 313,255

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Fibrous-long spacings (FLS) are one of atypical or artificial forms of collagen fibrils. We analyzed occurrence of this form of interstitial collagens of cervix uteri in pregnant, nonpregnant and post-partum white Wistar rats. Light and electron microscopy were used. Extensive collagenolysis in rat cervical stroma on the eve of labour and in close post-partum period was found. Extravasated activated eosinophils participated in this process. In the solid milieu of massive collagenolysis FLS were found. FLS were not found in nonpregnant cervix ureri. Administration of bleomycin had no influence on collagen degradation rate or on FLS formation.

617/2523

Tytuł oryginału: Synthesis, antiarrhythmic, and antihypertensive effect of novel 1-substituted pyrrolidin-2-one and pyrrolidine derivatives with adrenolytic activity.
Autorzy: Malawska Barbara, Kulig Katarzyna, Filipek Barbara, Sapa Jacek, Maciąg Dorota, Zygmunt Małgorzata, Antkiewicz-Michaluk Lucyna
Źródło: Eur. J. Med. Chem. 2002: 37 (3) s.183-195, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 312,629

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: A series of 1-substituted pyrrolidin-2-one and pyrrolidine derivatives were syntheised and tested for electrocardiographic, antiarrhythmic, and antihypertensive activity as well as for ŕ1- and ŕ2-adrenoceptors binding affinities. Among the newly synthesised derivatives several compounds with 3-(4-arylpiperazin-1-yl)propyl moiety displayed strong antiarrhythmic (7a-12a) and antihypertensive (7a-11a) activities. Compound 11a, 1-[2-acetoxy-3-[4-(2-methoxyphenyl)piperazin-1-yl]propyl]pyrrolidin-2-one, was the most potent in this series. The pharmacological results and binding studies that their antiarrhythmic and hypotensive effects may be related to their ŕ-adrenolytic properties, and that those properties depend on the presence of the 1-phenylpiperazine moiety with a methoxy- or chloro- substituent in the ortho position in the phenyl ring.

618/2523

Tytuł oryginału: Neonatal screening for cystic fibrosis.
Autorzy: Dodge John A.
Źródło: Pediatr. Pol. 2002: 77 (6) s.455-457, bibliogr. 9 poz.
Sygnatura GBL: 301,294

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

619/2523

Tytuł oryginału: The efficacy and safety of the tension-free vaginal tape procedure do not depend on he method of analgesia.
Autorzy: Adamiak Aneta, Milart Paweł, Skorupski Paweł, Kuchnicka Katarzyna, Nestorowicz Andrzej, Jakowicki Jerzy, Rechberger Tomasz
Źródło: Eur. Urol. 2002: 42 (1) s.29-33, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 312,572

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: The oryginal tension-free vaginal tape (TVT) method, described by Ulmsten et al., routinely uses local anaesthesia during the procedure. Since the anaesthetic effect after local application of lidocaine hydrochloride was not always satisfactory we decided to introduce the spinal anaesthesia during this operation. The aim of the present study was to compare local and spinal anaesthesia with respect to their efficacy and safety in the TVT procedure. Methods: 103 women, with objectively confirmed stress urinary incontinence, were randomised into the study. Sixty-seven women were anaesthetised locally and 36 patients spinally. All TVT procedures were performed as originally described. Objective assessment of the influence of anaesthesia on intra-abdominal pressure at rest and during the cough test was done using a rectal catheter and a central venous pressure manometer. The efficacy of the TVT procedure was based on a gynaecological examination with a cough test and a three-degree subjective scale: complete cure, improvement or failure. Results: The success of the TVT procedure performed under local anaesthesia is a comparable with hat achieved under spinal analgesia (p = 0.42). The number of complications that occurred in the two groups does not differ significantly (p = 0.57). Conclusions: Spinal anaesthesia impairs the ability to cough effectively during the TVT procedure. However, the efficacy and safety of the operations performed under this type of anaesthesia are comparable with the efficacy and safety of operatins done under local anaesthesia.

620/2523

Tytuł oryginału: Cockroach allergy and exposure to cockroach allergen in Polish children with asthma.
Autorzy: Stelmach I., Jerzyńska J., Stelmach W., Majak P., Chew G., Górski P., Kuna P.
Źródło: Allergy 2002: 57 (8) s.701-705, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 302,302

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bacgriund: Asthma morbidity increases every year, especially among children, and exposure to high levels of indoor allergens is a very important factor. We evaluated the prevalence and exposure to cockroach (CR) allergen in asthmatic children in Poland, and also tested the hypothesis that asthma with allergy to CR is more severe than with allergy to other antigens. Methods: One hundred and sixty children with asthma were examined, had skin prick tests (SPT) with common and CR allergens, underwent spirometry, and provocation tests to histamine. Children with positive SPT to CR had measured specific IgE levels to this antigen and Bla g 2 concentrations were measured in their homes. Results: The most common allergen, was dust mite 51.3 p.c., followed by pollen 48.8 p.c. and CR allergen 24.3 p.c. In children with CR sensitivity, 13 p.c. had mild ashma, 26 p.c. moderate and 61 p.c. had severe asthma. Their levels forced expiratory volume in one second (FEV1), and te provocative concentration of histamine that caused a 20 p.c. fall in FEV1 (PC20), were statistically lower than in the group of children with other than CR allergies. Bla g 2 antigen was detected in 55.13 p.c. samples. The highest levels of Bla g 2 were found in old houses, without central heating, and in houses with lower income. Conclusion: In Polish children, CR allergen is a very important factor of sensitivity. Concentratins of Bla g 2 in homes are higher than previously reported in other European countries, and are strongly related to the houses' characteristics. Also, children with CR hypersensitivity have severe asthma more often than children with other allergies.

621/2523

Tytuł oryginału: Urinary leukotriene levels are increased during exacerebation of atopic eczema/dermatitis syndrome. Relation to clinical status.
Autorzy: Adamek-Guzik T., Guzik T. J., Czerniawska-Mysik G., Korpanty G., Mastalerz L., Radwan J., Szczeklik A.
Źródło: Allergy 2002: 57 (8) s.732-736, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 302,302

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Leukotrienes are potent mediators of allergic inflammation and their role in the pathogenesis of allergic disorders, particularly asthma, is well established. Their importance in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS) is still unclear. We aimed to compare urinary cysteinyl leukotriene (Cys-LT) levels during exacerbation and remission of AEDS in relation to clinical status, IgE levels, and eosinophil counts. Methods: Urinary Cys-LTs were measured by direct enzyme immunoassay in 17 adult patients with AEDS and in 17 healthy controls in whom atopy had been excluded. Cys-LTs were compared during exacerbation and remission of AEDS in relation to the clinical status measured by SCORAD. Total IgE levels were measured by enzyme-linked immunoassay (ELISA). Results: Mean clinical score during the exacerbation was 64.3 ń 3.1 and during remission 22.4 ń 4 (P 0.01). Cys-LTs were significantly higher during the exacerbation of AEDS than in the control group (230.9 ń 20.8 vs 123.2 ń 9.9 pg/mg creatinine; P 0.005). During the remission, the difference between AEDS patients and the control group was not significant (96.3 ń 8.7 vs 123.2 ń 9.9 pg/mg creatinine; P = 0.8). During AEDS exacerbation Cys-LTs levels were significantly correlated with the clinical status (rs = 0.73, P 0.01) and with eosinophil counts (r = 0.86; P 0.01) but not with the duration of the disease, age of patients, or IgE levels. Conclusions: Our results point to enhanced biosynthesis of Cys-LTs during the AEDS exacerbations. Inflammatory cells, e.g. eosinophilis are the most...

622/2523

Tytuł oryginału: Activity of class I, II, III, and IV alcohol dehydrogenase isoenzymes in human gastric mucosa.
Autorzy: Jelski Wojciech, Chrostek Lech, Szmitkowski Maciej, Laszewicz Wiktor
Źródło: Dig. Dis. Sci. 2002: 47 (7) s.1554-1557, tab., bibliogr. 19 poz.
Sygnatura GBL: 310,037

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The gastric alcohol dehydrogenase (ADH) plays an important role in the "first pass" metabolism of ethanol. Human ADH exists in multiple forms, grouped into six classes and located in different tissues. In present study we investigated the activity of our four classes (I, II, III, and IV) of alcohol dehydrogenase isoenzymes in the different parts of stomach (corpus and antrum) in patients with suspected gastric ulcer. The aim of the study was assess the particular role of different classes of ADH in the gastric dehydrogenase actvity. For the measurement of the activity of class I and class II isoenzymes, we employed new fluorometric methods with specific substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method with n-octanol and class IV with m-nitrobenzaldehyde as a substrate, respectively. All biopsy specimens were taken from less changed areas of the antrum and body of the stomach pof 68 patients suspected of having gastric ulcer. It was found that ADH IV (gastric) activity was the highest (14.76 ń 0.68 in the corpus of the stomach in men; and 7.61 ń 0.68 in women, respectively). The activity of the ADH III isoenzyme was lower than that of ADH IV. The activities of class I and II ADH isoenzymes were barely detectable. All tested classes of ADH had higher activity in the corpus than in the antrum and in males than in females. In conclusion, the most important form of gastric ADH is isoenzyme of class IV, less important is the isoenzyme of class III. ADH classes I and II seem to have no role in the stomach.

623/2523

Tytuł oryginału: Corticotropin-releasing hormone affects cytokine production in human HaCaT keratinocytes.
Autorzy: Zbytek Błażej, Myśliwski Andrzej, Słomiński Andrzej, Wortsman Jacobo, Wei Edward T., Myśliwska Jolanta
Źródło: Life Sci. 2002: 70 (9) s.1013-1021, il., bibliogr. 31 poz.
Sygnatura GBL: 304,948

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: CRH cutaneous expression is significantly enhanced after exposure to various stimuli (Physiol Rev 2000,80;979-1020). We evaluated the effect of CRH on cytokine production in HaCaT keratinocytes, a cell line shown to express CRH receptors coupled to cAMP activation and calcium-dependent transmission pathways. It is demonstrated for the first time that exogenously added CRH stimulates production of IL-6 and IL-11. It also inhibits production of IL-1beta and does not affect TNF-alpha production. Our results indicate that CRH functioh(s) during cutaneous stress may be mediated by differential effects on cytokine production.

624/2523

Tytuł oryginału: Indomethacin protects against neurodegeneration caused by MPTP intoxication in mice.
Autorzy: Kurkowska-Jastrzębska I., Babiuch M., Joniec I., Przybyłkowski A., Członkowski A., Członkowska A.
Źródło: Int. Immunopharmacol. 2002: 2 (8) s.1213-1218, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 313,577

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The anti-inflammatory agents are postulated to be effective in treating neurodegenerative disorders. In this study, we showed that indomethacin (IND) in the dose of 1 mg/kg protected neurons against toxic damage caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice model of Parkinson's diseases. IND also diminished microglial activation and lymphocytic infiltration in the injured areas. These observations suggest that anti-inflammatory properties of IND may play a role in the neuron's protection in this model. However, diminished inflammatory reaction may be secondary to less neuronal damage.

625/2523

Tytuł oryginału: Concentration and composition of free ceramides in human plasma.
Autorzy: Górska M[aria], Dobrzyń A., Żendzian-Piotrowska M., Namiot Z.
Źródło: Horm. Metab. Res. 2002: 34 (8) s.466-468, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 312,226

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

626/2523

Tytuł oryginału: Biological properties of a new fluorescent biphalin fragment analogue.
Autorzy: Lipkowski Andrzej W., Misicka Aleksandra, Kosson Dariusz, Kosson Piotr, Lachwa-From Magdalena, Brodzik-Bieńkowska Agnieszka, Hruby Victor J.
Źródło: Life Sci. 2002: 70 (8) s.893-897, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 304,948

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Previous studies of stsurcture-activity of biphalin defined fragments which expressed the full biological potency of the parent compound. The most simple fragment was Tyr-D-Ala-Gly-Phe-NH-HH - X, where X = Phe, but it also could be other hydrophobic amino acids. This paper presents data that replacement of the phenylalanine with a dansyl (X = DNA) groups gives an analogue (AA20116) that fully preserves the high affinity of the initial analogue for both ć and ë opiioid receptors. In the tail flick test in rats, intrathecal injection of the compound produces strong antinociception, comparable to the parent biphalin. Cecause AA2016 contains a strong fluorescent group, it can be a very useful tool for prospective studies in vivo, including biological barrier permeability, tissue distribution, metabolism and receptor-ligand complex formation.

627/2523

Tytuł oryginału: Superficialization of the wrist native arteriovenous fistula for effective hemodialysis vascular access construction.
Autorzy: Weyde Wacław, Krajewska Magdalena, Letachowicz Waldemar, Klinger Marian
Źródło: Kidney Int. 2002: 61 (3) s.1170-1173, il., bibliogr. 10 poz.
Sygnatura GBL: 312,423

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background. It is commonly known that the best type of vascular access for chronic hemodialysis, defined as the one with the lowest number of complications and longest use, is the primary (Brescia-Cimino) radiocephalic fistula. One reason for the impossibility of its creation is that the vein vessel on the forearm is located too deeply, which is primarily caused by the patients' obesity. Methods. In 24 obese patients the superficialization procedure of the venous part of fistula under the skin was performed. Results. In 23 patients of this group, an easy and uncomplicated fistula puncturing was achieved. One patient had the complication of fistula clotting. Conclusion. Subcutaneous transposition of the arterialized median antebrachial vein is a simple and effective method of an adequate distal native fistula creation in patients with deeply located forearm veins.

628/2523

Tytuł oryginału: Fibroblast-like synoviocytes from rheumatoid arthritis patients express functional IL-15 receptor complex: endogenous IL-15 in autocrine faschion enhances cell proliferation and expression of Bcl-xL and Bcl-2.
Autorzy: Kurowska Mariola, Rudnicka Weronika, Kontny Ewa, Janicka Iwona, Chorazy Magdalena, Kowalczewski Jacek, Ziółkowska Maria, Ferrari-Lacraz Sylvie, Strom Terry B., Maśliński Włodzimierz
Źródło: J. Immunol. 2002: 169 (4) s.1760-1767, il., bibliogr. 49 poz.
Sygnatura GBL: 300,982

Hasła klasyfikacyjne GBL:

reumatologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The hallmarks of rheumatoid arthritis (RA) are leukocytic infiltratin of the synovium and expansiveness of fibroblast-like synovicytes (FLS). The abnormal proliferation of FLS and their resistance to apoptosis is mediated, at least in part, bay present in RA joints proinflammatory cytokines and growth factors. Because IL-15 exerts properties of antiapoptotic and growth facators, and is produced by RA FLS, we hypothesized that IL-15 participates in RA FLA activation. To test this hypothesis, we first examined whether RA FLS express chains required for high affinity functional IL-15R. Indeed, RA FLS express IL-15Rŕ at mRNA and protein levels. Moreover, we confirmed the presence of IL-2Rá and common ç-chains. Interstingly, TNF-ŕ or IL-1á triggered significant elevation of IL-15Rŕ chanin at mRNA and protein levels. Next, we investigated the effects of exogenous or endogenous IL-15 on Bcl-2 andBcl-XL expression, FLS proliferation, and apoptosis. Exogenous IL-15 enhanced RA FLS proliferation and increased the level of mRNA-encoding Bcl-XL. To test the role of endogenous IL-15 in the activation of RA FLS, an IL-15 mutant/Fcç2a protein exerting properties of specific antagonist to the IL-15Rŕ chain was used. We found that blocking IL-15 biological activities using this protein substantially reduced endogenous expression of Bcl-2 and Bcl-XL, and RA FLA proliferation that was reflected by increased apoptosis. Thus, we havae demonstrated that a distinctive phenotype of RA FLS, i.e., persistent activation, proliferation, and resistance to apoptosis, is related to the autocrine activation of IL-15Rs by FLS-derived IL-15.

629/2523

Tytuł oryginału: The mechanism of the hemolytic activity of polyene antibiotics.
Autorzy: Knopik-Skrocka Agnieszka, Bielawski Józef
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.31-48, il., tab., bibliogr. 49 poz.
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The kinetics of the filipin-, amphotericin B- and nystatin-induced hemolysis of human erythrocytes were investigated. Filipin-induced hemolysis is of the damage type. It is an all-or-none process, partly inhibited by Ca2+ or Ba2+ abut not bay Mg2+, Na+ or SO42-. The hemolytic activity of filipin is explained by the formation of large aggregates within the erythrocyte membrane in the form of large perforations, permeable to substances of low molecular weight as well as to macromolecules, including hemoglobin. In isotonic KCl solution, both amphotericin B and nystatin, at low concentrations, form smaller aggregates within the membranes. As a result, the permeabiality of teh membranes to KCl increases and hemolysis occurs. However, the kinetics of the hemolysis induced by the two polyenes is complex. The process shows some features of the permeability type and some of the damage type. It is suggested that amphotericin B and nystatin may simultaneously form a number of transport systems, differing in their molecular organisation and hemolytic activity. Their participation in erythrocyte membrane permeability can be modified by small changes in membrane organisation and the chemical composition of the incubation medium. In isotonic solutions of divalent cation chlorides, and at higher antibiotic concentration, additional aggregates, allowing divalnt cations to permeate, appear. These structures do not permit SAO42- to permeate.

630/2523

Tytuł oryginału: The International Confrence on Life Sciences - 2001, September 22.-26.2001, Gozd Martuljek, Slovenia.
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.69-174, il., tab., bibliogr. [przy ref.] - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

631/2523

Tytuł oryginału: The biomechanics of standing and balancing in paralyzed people.
Autorzy: Bajd Tadej, Mihelj Matjaz, Matjacić Zlatko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.74-77, tab., bibliogr. 6 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Prolonged immobilization results in several physiological problems. It has been demonstrated that standing exercises can ameliorate many of these problems. Standing exercises can be performed efficiently with the help of functional electrical stimulation (FES). A novel robotic mechanism which aids the unsupported standing of paraplegics, providing balancing exercise has been developed. The balancing strategy is based on voluntary activity of the paraplegic's upper body and artificially controlled stiffness in the ankles.

632/2523

Tytuł oryginału: Magnetic resonance imaging of arterial thrombi and its possible correlation to fibrinolytic treatment.
Autorzy: Kozak Matija, Blinc Ales, Sersa Igor, Mikac Ursa, Surlan Milos
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.82-86, il., tab., bibliogr. 7 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: For 13 patients with subacute and 4 patients with chronic occlusion, magnetic resonance imaging (MRI) of occlusive arterial thrombi in the superficial femoral artery were performed in vivo. The patients with subacute occlusion were treated with cetheter-directed thrombolysis. The frequency of MR signal intensity and its distribution in thrombi were studied for 11 successully adn 2 unsuccessfully treated patients and patients with chronic occlusion. Intra-araterial thrombi were MRI inhomogenous in all of the patients, abut the MR signals from lysable and chronic thrombi were significantly different than those from nonlysable ones. The MRI of occusive arterial thrombi is probably usaable to predict the therapeutic outcome of thrombolytic treatement.

633/2523

Tytuł oryginału: Structural studies on membrane proteins using non-linear spin label EPR spectroscopy.
Autorzy: Pali Tibor, Marsh Derek
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.87-91, il., bibliogr. 11 poz. - Międzynarodow konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Non-linear electron spin resonance (EPR) techniques suitable for measuring proximity relationships in membranes are reviewed. These were developed during the past decade in order to measure changes sensitively in the spin-lattice relaxation time (T1) of nitroxyl spin labels covalently attached to membrane lipids or proteins. In combination with paramagnetic quenching agents and double spin-labelling, the methods were further developed for distance measurements. Selected examples are given to illustrate different methods, and types of data obtained for both integral and peripheral membrane.

634/2523

Tytuł oryginału: Functional MDR1 polymorphisms (G2677T and C3435T) and TCF4 mutations in colorectal tumors with high microsatellite instability.
Autorzy: Potocnik Uros, Ravnik-Glavac Metka, Glavac Damjan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.93-95, tab., bibliogr. 7 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The multidrug resistance 1 (MDR) gene and transcription factor 4 (TCF4) gene are suggested to be involved in the WNT signalling pathwey, the most important patway altered in colorectal cancer. Mutations in both genes have been identified and associated with colorectal tumors exahibiting high microsatellite instability (MSI-H). In this study, we report on the distribution of functional polymorphism in the MDR1 gene and somatic frameshift mutations in the TCF4 gene coding mononucleotide repetition in 62 MSI-H colorectal tumors. Somatic frameshift mutations in(of) the TCF4 gene were identified in 24/62 (39 p.c.) of the studied MSI-H tumors. The estimatd allele frequencies of functional polymorphisms in(of) exon 21 (2677 G T, Ala893Ser) and exon 26 (3435 C T, Ile1142Ile) of the MDR1 gene were 0.42 and 0.46 in the controls and 0.54 (p = 0.035) and 0.60 (p = 0.017) in the MSI-H tumors. However, the allele frequency of both functional MDR1 polymorphisms did not significantly differ between MSI-H tumors with TCF4 mutations and those without. These results support the involvement of the MDR1 gene in the tumorgenesis of MSI-H tumors and also suggest that functional polymorphisms in the MDR1 gene and mutations in the TCF4 gene are likely to occur independently in MSI-H tumors.

635/2523

Tytuł oryginału: A generalized mathematical model of biological oscillators.
Autorzy: Ton-That Tuong
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.101-104, bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Biological rhythms such as circadian rhythms, biochemical rhythms and neural oscillators are based on the mathematical model of the theory of harmonic oscillators. These are solutions of certain second-order differential equations. They can also be viewed as spherical harmonics on the circle in the two-dimentsional Euclidean space. The sphrerical harmonics on (n = 1)-spheres and, more generally, the Stiefel harmonics can represent oscillatory phenomena, and we expect that they can serve as models for more complex biological rhythms.

636/2523

Tytuł oryginału: Intravascular plug formation induced by poly-APS is the principal mechanism of the toxin's lethality in rats/rat tissues.
Autorzy: Bunc Matjaz, Sarc Lucija, Rozman Janez, Turk Tom, Sepcic Kristina, Suput Dusan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.106-108, bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Toxic water solubale polymeric 3-alkylpyridinum salts isolated from the sponge Raniera sarai strongly inhibited AChE in vitro. In vivo, experimental animals died due to plugs formed in microcirculation. The mechanism of this plug formation is unknown. In vitro, the toxin did not affect the coagulation rate, but the rate of platelet aggregation was accelerated in a dose-dependent manner. The hemolytic activity of poly-APS was diminishe by the addition of serum proteins in a dose-dependent manner. These results support the conclusion that non-specific bainding to proteins is the underlying mechanism of the lethality of poly APS.

637/2523

Tytuł oryginału: The influence of the biomechanical parameters of the hip on the outcome of treatment of hips subject to avascular necrosis of the remoral head.
Autorzy: Dolinar Drago, Kralj-Iglic Veronika, Iglic Ales, Antolic Vane, Herman Srecko, Pavlovcic Vinko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.109-111, il., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: We were interested in whether or not the biomechanical status of the hip influences the course of avascular necrosis of the femoral head. To invaestigate this, we used a computer aided system based on a three dimensional mathematical model for determining the stress distribution in the hip joint from standard anterioposterior rentgenographs(X-ray images) of both hips and pelvis. Based on the results of our study, we suggest that the biomechanical parameters of the hip play an important role in the outcome of treatment of hips affected by avascular necrosis of the femoral head.

638/2523

Tytuł oryginału: Examination of fragment dose contribution in heavy ion radiotherapy.
Autorzy: Giacomelli Marko, Ilic Radomir, Skvarc Jure
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.112-114, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Heavy-ion radiotherapy is an efficient mthod for the treatment of deep-seated tumors, because the stopping of ions in a tissue delivers the maximal absorbed dose to the tumor-affected areas with minimal damage to the healthy tissues. However, heavy ions can undergo nuclear reactions, giving products with lower Z-values and hence a longer range in the tissue. This causes a dose increase beyond the mean range of the primary beam. The contribuation of such reaction products was examined in an experiment where a stack of tissue-like targets interleaved with CR-39 etched traack detectors (ETD) was irradiated with heavy ions. The analysis was performed using a recently developed technique of trajectory tracing, which enables the spectroscopy of fragments with different Z-values.

639/2523

Tytuł oryginału: Frequency encoding of intracellular Caý+ signals.
Autorzy: Grubelnik Vladimir, Marhl Marko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.115-117, il., bibliogr. 6 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In a very recent theoretical study [1], we showed that simple mitochondrial kinetics, originally proposed by Marhl et al. [2], could be easily used as a plug-in element in other models desribing intracellular Ca2+ dynamics. We analysed several previously published models and showed that mitochondrai could indeed maintain the constant amplitude of intracellular Ca12+ oscillations very effectively. We also pointed out the importance of amplitude regulation for the frquency encoding of intracaellular Ca2+ signals. This paper focuses on giving a more exhaustive demonstration of this phenomenon of frequency encoding for the model of Dupont et al. [3].

640/2523

Tytuł oryginału: Cyclophosphamide metabolites perforate the apical plasma membranes of urothelial cells in rats.
Autorzy: Koprivec Dasa, Jezernik Kristijan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.123-125, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The apical plasma membrane of differentiated superfical urothelial cells is characterised by the presence of asymmetric unit membrane (AUM). Cyclophsphamide (CP) metaabolites cause perforation of these thickened membranes. In this study, apical plasma membranes were examined after CP injection by electron microscopy. The immediate effect of the CP metabolites was observed as small round holes appearing, first in the asymmetric apical plasma membrane of terminally differentiated superficail cells, and later in the symmetric apical plasma membrane of exposed undifferentiated intermediate and basal cells. Exposed cells which remained undamaged, immediately underwent maturation of the symmetric apical plasma membarane. These results indicate that CP metabolites perforate the symmetric and asymmetric membranes of most urothelial cells.

641/2523

Tytuł oryginału: The small world in biophysical systems structural properties of glycolysis and the TCA cycle in Escherichia coli.
Autorzy: Krajnc Borut, Marhl Marko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.129-131, tab., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: It has been shown that the central metabolic network of Escherichia coli is of the small-world type. In this paper, we present that the metabolic network of glycolysis and TCA cycle as a part of the E. coli metabolism is also a small-world network. We found that the hubs of the studied network are consistent with those found in the complete metabolic network. The evolutionary meaning of this finding is discussed.

642/2523

Tytuł oryginału: Sphericity of the femoral head on anterior-posterior radiographs of dysplastic hips.
Autorzy: Mavcic Blaz, Antolic Vane, Iglic Ales, Kralj-Iglic Veronika
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.132-133, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: We analyzed the spehericity of the femoral head of dysplastic hips. Using standard anterior-posterior radiographs of the hips, we assessed the femoral head's deviation from a spherical shape using a computer algorithm and via Severin grading. The method presented could serve as a useful tool to quantify differences in sphericity in cases where it is difficult to grade the hip radiologically.

643/2523

Tytuł oryginału: Electric current density imaging of tablet dissolution.
Autorzy: Mikac Ursa, Demsar Alojz, Sersa Igor, Demsar Franci
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.136-138, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The Electric current density imaging technque (CDI) was used to monitor the dissolution of and ion migration from tablets of different acids in agar-agar gel. Conventional MRI cannot monitor these processes, since it can only show changes in the size of the tablet during the dissolving process. CDI traces the dissolfed ions thanks to changes in conductivity.

644/2523

Tytuł oryginału: Renal injuries induced by chronic intoxication with microcystins.
Autorzy: Milutinovic Aleksandra, Sedmak Bojan, Horvat-Znidarsic Irena, Suput Dusan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.139-141, il., bibliogr. 2 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The microcystins (MCs) LR and YR are hepatoxins produced by some species of freshwater cyanobacteria. Only a few reports on the acute nephrotoxicity of MCs have been published to date. Here we investigate the effecats on rat kidneys of chronic administration of relatively low doses of MC LR and MC YR. Male Wistar rats wer injected every second day for 8 months with MC LR (10 ćg/kg i.p., n = 5) and MC YR (10 ćg/kg i.p., n = 5). The control group was treated with vehicle, a mixture containing 0.8 p.c. ethanol and 0.2 p.c. methanol in 0.9 p.c. saline (3.7 ml/kg i.p., n = 5). We found that MCs could induce damage to the kidney cortex and medulla. The lesions mainly consisted of damaged and dilated tubuli filled with homogenous eosinophil material. We conclude that long-term exposure to relatively low doses of MCs poses a considerable risk for kiney injury.

645/2523

Tytuł oryginału: EPR spectroscopy of common nitric oxide - spin trap complexes.
Autorzy: Nedeianu Saviana, Pali Tibor
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.142-143, bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Two commonly used hydrophobic and hydrophilic spin traps for NO, namely Fe2+ (DETC)2 and Fe2+ (MGD)2, respectively, were analyzed via EPR spectroscopy. EPR spectra of trapped NO, together with field position standards, were recorded both in the frozen state and at room temperature. We present a detailed characterization of the EPR spectra of the above paramagnetic NO complexes, concerning g-value, hyperfine splitting and linewidths. This study also provides spectroscopic data required to develop a quantitative and sensitive detection system for nitric oxide both in hyrophobic and hydrophilic aqueous media.

646/2523

Tytuł oryginału: How should dysplastic human hips be evaluated?
Autorzy: Pompe Borut, Antolic Vane, Iglic Ales, Jaklic Anton, Kralj-Iglic Veronika, Mavcic Blaz
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.144-146, tab., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Dysplasia of the human hip is characterised bay insufficient anterolateral covering of femoral head by the acetabulum. In our study, we evaluated dysplastic human hip joints using biomechanical parameters (the peak contact stress in the weight-bearing area of the hip - pmax) and X-ray image parameters (the centre-edge angle - VCE, the transverse acetabular inclination angle - VUS, the acetabaular index of the weight-bearing zone - VAC, the ACM angle - VACM, and the hip value - HV). The purpose of this study is to make use of X-ray and biomechanical parameters to evaluate hips diagnosed with "hip dysplasia", and to establish whether or not ther is a correlation between the two. Our results show a statistically significant correlation between pmax and VCE, VUS and VAC. The correlation between pmax and VACM and HV is not statistically significant.

647/2523

Tytuł oryginału: The relationship between innervation density and dynamic balance function.
Autorzy: Rugelj Darja
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.149-150, bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Functional reach (FR) significantly increases with additional sensory information from the fifth metacarpal surface of the dominant hand. The purpose of this study was to determine the relationship between the innervation density of the skin above the fifth metacarpal surface of the palm and the increase of FR with an additional light touch. The results show a moderate negative correlation between innervation density and the increase of FR with light touch.

648/2523

Tytuł oryginału: The influence of amino acaid side chains on water binding to the copper(II) in bis(N,N-dimethyl-L-ŕ-isoleucinato)-copper(II): and EPR and molecular mechanics study.
Autorzy: Sabolovic Jasmina, Noethig-Laslo Vesna
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.151-153, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Simulations were done of the electron paramagnetic resonance (EPR) spectra for bis(N,N-dimethyl-L-ŕ-isoleucinato)copper(II) dissolved in deuterated methanol as a function fo temperature. They indicated different behaviour of the complex below and above 300řK. The effect was examined by the conformational analysis of the copper(II) complex with a new molecular mechanics force field.

649/2523

Tytuł oryginału: Quantification of the MR images of hydrogels by NMR relaxation measurements.
Autorzy: Sepe Ana, Baumgartner Sasa, Kristl Julijana, Lahajnar Gojmir, Jarh Orest
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.154-156, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Layers of hydrogael respresent a diffusional barrier that retards the process of drug release. For better prediction of drug release, a method for evaluating the polymer concentration profile was developed.

650/2523

Tytuł oryginału: Heart rate variability - a shape analysis of lorenz plots.
Autorzy: Sevsek France, Jakovljevic Miroljub
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.159-161, bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: A new quantitative geometrical method to analyse heart rate variability is presented. The standard image analysis techniques were applied to the grey scale images of Lorenz plots, and the outlines of the attractor areas were determined via a contour following procedure based on a maze walking algorithm. For graphs of simple compaact shape, the attractor region contours were described in terms of Fourier coefficients.

651/2523

Tytuł oryginału: Selective radigraphy of 10B distribution in organs using cold and thermal neutron beams.
Autorzy: Skvarc Jure, Giacomelli Marko, Yanagi Hironobu, Khne Guido
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.162-164, il., bibliogr. 2 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The investigation of boron biodistribution by neutron induced autoradiography was performed using a CR-39 etched track detector. Calibration samples, made of boronated chicken liver, and freeze-dried mouse tissue samples were irradiated both with thermal and cold neutrons. Digital images were made on the basis of track densities, with the selection of tracks due to 10BB(n, ŕ)7Li reaction. A comparison of results obtained both with thermal and cold neutrons demonstrated that it is possible to obtain equivalent results with both neutron sources, although the background noise due to recoil protons from the fast neutrons of the thermal neutron source is 15 p.c. higher than that of the cold neutron source. 10B concentrations in the range of 0.5 ppm to 150 pm were determined, with a standard deviation of 13 p.c. and 8 p.c. for the mouse tissue samples and calibration samples, respectively.

652/2523

Tytuł oryginału: The influence of Na+ ions on histamine secretion from mast cells induced by NGF or compound 48/80.
Autorzy: Stempelj Mateja, Carman-Krzan Marija, Ferjan Ilonka
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.165-166, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: It has been demonstrated that the release of histamine from mast cells by cytokines is strongly dependent on extracellular Ca2+ and Na+ ions. The results of our current research indicate that the removal of extracellular Na+ enhances NGF induced histamine release, but reduces induction by compound 48/80, suggesting that different mechanisms ar involved in the secretory process induced bay these agents.

653/2523

Tytuł oryginału: The effect of proprioceptive training on neuromuscular function in patients with patellar pain.
Autorzy: Strojnik Vojko, Vengust Rok, Pavlovcic Vinko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (1) s.170-171, il., bibliogr. 2 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze 2001 Gozd Martuljek 22-26.09. 2001
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Eight patients with patellar pain underwent knee proprioceptive training. The maximal knee extension torque associated with the Vastus Lateralis EMG signal increased (p 0.001 and 0.039). Although muscle balance was not improved, all the patients improved their clinical scores.

654/2523

Tytuł oryginału: Disposition and metabolism of 1,6-dimethylnaphthalene in rats.
Autorzy: Kilanowicz Anna, Sapota Andrzej, Czerski Bogdan
Źródło: Toxicol. Lett. 2002: 134 (1/3) s.227-243, il., tab., bibliogr. [18] poz.
Sygnatura GBL: 306,158

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The distribution, excretion and metabolism of 1,6-dimethylnaphthalene following i.p. administration of a single dose of 20 mg/kg to rats, was investigated using radiotracer [3H] and a gas chromatography - mass spectrometry technique (GC-MS). After 72 h, about 94 p.c. of the given dose was excreted in urine and feces. In organs and tissues, the highest concentration during the first hours after administration was detected in fat, liver, spleen and kidneys. Then gradual decline of tritium was noticed in all examined tissues. In urine, the following substanceswere identified and quantified by GC peak areas: unchanged 1,6-dimethylnaphthalene, 1-methyl-hydroxynaphthalenes, 1-hydroxymethyl-6-methylnaphthalene, 1,6-dimenthyl-thionaphthalene, 6-methyl-1-naphthoic aldehyde, 6-methyl-1-naphthoic acid, 1,6-dimethyl-thionaphthalene and 1,6-dimethyl-methylthionaphthalene.

655/2523

Tytuł oryginału: Acute and subacute nephrotoxicity of 2-bromophenol in rats.
Autorzy: Bruchajzer Elżbieta, Szymańska Jadwiga A., Piotrowski Jerzy K.
Źródło: Toxicol. Lett. 2002: 134 (1/3) s.245-252, il., tab., bibliogr. [24] poz.
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The present report aims at providing broader information on the acute nephrotoxicity of 2-bromophenol (2-BP) (a bromobenzene (BB) metabolite), due to its action on the kidneys, after repeated administration. Investigations were performed on female rats. Following a single dose, the most pronounced changes involved: concentrations and rates of excretion of proteins in urinwe, the number of epithelial cells excreted in urine, creatinine and urea clearance and reduced gluthione in renal tissue. Immediate effects could be ascribed to both renal tubules and glomeruli, mirrored in the level of urinary proteins and intensified excretion of renal epithelial cells. Less pronounced changes of the indicator values were noted under repeated dosing of 2-BP. The results obtained in a single exposure study confirm earlier on the mild nephrotoxicity of 2-BP following exposure to high doses. However, the transition from single to repeated expopsure does not result in enhanced nephrotoxicity.

656/2523

Tytuł oryginału: Relationship between hyperinsulinemia and angiographically defined coronary atherosclerosis in non-diabetic men.
Autorzy: Kruszelnicka-Kwiatkowska O., Surdacki A., Goldsztajn P., Matysek J., Piwowarska W., Golay A.
Źródło: Diabetes Metab. 2002: 28 (4 c. 1) s.305-309, tab., bibliogr. 24 poz.
Sygnatura GBL: 313,123

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background. Our aim was estimate the relationship between hyperinsulinemia and angiographically validated coronary atherosclerosis. Methods: 102 consecutive non-diabetic male subjects (age 48.9 ń 9.6 years) with a body mass index 25.9 ń 2.8 kg/mý referred to coronary angiography in our centre were studied. A 75-g oral glucose tolerance test (OGTT) was performed and the areas under the curve (AUC) of serum insulin and glucose were calculated. Results: Discriminant analysis with stepwise forward variable selection revealed that in younger patients ( 50 years), the following parameters affected positively the number of significant coronary lumen reductions: age (F = 8.5, p = 0.005). InAUCinsulin (F = 5.8, p = 0.02), low HDL cholesterol (F = 4.4, p =n 0.04), the smoking habit (F = 4.1, p = 0.05). In those 50 years of age, exclusively age (F = 5.8, p = 0.02) and hyperuricemia (F = 3.8, p = 0.06) entered of the final model. Conclusion: Our results indicate that in non-diabetic male subjects the association of hyperinsulinemia with the severity of coronary arterosclerosis is only seen in younger patients.

657/2523

Tytuł oryginału: Synchytrium endobioticum and its potential role in carcinogenesis
Autorzy: Marcinkowski Tadeusz
Źródło: - Goleniów, Oficyna Wydaw. "BIOS" 2002, 25 s. : il., bibliogr. 4 poz., 21 cm.
Sygnatura GBL: 660,759

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Łuczyński

Stanisław 1902-1971

658/2523

Tytuł oryginału: In situ activation of caspases and serine proteases during apoptosis detected by affinity labeling their enzyme active centers with fluorochrome-tagged inhibitors.
Autorzy: Grabarek Jerzy, Darzynkiewicz Zbigniew
Źródło: Exp. Hematol. 2002: 30 (9) s.982-989, il., bibliogr. 60 poz.
Sygnatura GBL: 305,749

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Activation of caspases is the key event of apoptosis. To detect this event in situ we applied fluorochrome-labeled inhibitors of caspases (FLICA) as affinity of activity centers of these enzymes. The FLICA are fluoroscein- or sulforhodamine-conjugated peptide-fluoromethyl ketones that covalently, with 1:1 stoichiometry, bind to enzymatic centers of caspases; the specifity is provided by the peptide sequence of amino acids. Similarly, we applied fluorescent inhibitors of serine proteases (FLISP) to detected activite sites of the latter enzymes. Exposure of live cells to FLICA of FLISP led to uptake of these ligands and their binding to activated caspases or active sites og serine proteases; the unbound reagents were removed by cell rinse. Only cells undergoing apoptosis were labeled with FLISP or FLICA. Intracellular binding sites of FLICA are consistent with known localization of caspases. Covalent binding of FLICA or FLIPPS allowed us to identify the labeled proteins by immunoblotting: the proteins that bound individual FLICAs had molecular weight between 17 and 22 kDa, which corresponds to large subunits of the caspases; two proteins reacting with FLISP were about 57 and 60 kDa, which suggests that they are novel enzymes. Detection of caspases or serine proteases activation can be combined with other markers of apoptosis or cell cycle for multiparametric analysis by flow or laser ascanning cytometry. Being caspases inhibitors, FLICA arrest the process of apoptosis and prevent cell distintegration. The stathmo-apoptotic assay was developed...

659/2523

Tytuł oryginału: Gastric syphilis - a case report.
Tytuł polski: Kiła żołądka - opis przypadku.
Autorzy: Poniewierka Elżbieta, Paradowski Leszek, Sajewicz Zbigniew, Jeleń Michał
Źródło: Gastroenterol. Pol. 2002: 9 (1) s.71-73, il., bibliogr. 7 poz., streszcz.
Sygnatura GBL: 313,180

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Kiłowe zapalenie żołądka jest obecnie rozpoznawane bardzo rzadko. Objawy kliniczne i wyniki endoskopii często wskazują na raka żołądka lub chłoniaka. Rozpoznanie jest często przypadkowe, wsparte pozytywnymi wynikami testów serologicznych w kierunku zakażenia kiłowego.

Streszczenie angielskie: Syphilitic gasttritis is now recognized extremely rarely. Clinical symptoms and endoscopic findings are not characteristic and often suggest gastric carcinoma or lymphoma. Diagnosis is often casual, supported by positive findings of common serologic tests for syphilis.

660/2523

Tytuł oryginału: Endothelin-1 (ET-1) as a mediator of pregnanycy induced hypertension (PIH).
Autorzy: Heimrath Jerzy, Krawczenko Agnieszka, Duś Danuta
Źródło: Ginekol. Pol. 2002: 73 (6) s.512-516, il., tab., bibliogr. 14 poz., streszcz.
Sygnatura GBL: 301,184

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie polskie: Występowanie nadciśnienia indukowanego ciążą (PIH - pregnancy induced hypertension) jest związane z uszkodzeniem i następującą aktywacją komórek śródbłonka naczyniowego łożyska. Aktywowane komórki śródbłonkowe produkują cały szereg mediatorów, wśród których na szczególną uwagę zasługuje endotelina-1 (ET-1) - jeden z głównych czynników wywołujących skurcz naczyń. Celem naszych badań było określenie poziomu ET-1 w surowicy 18 pacjentek z PIH w porównaniu z grupą 16 zdrowych ciężarnych. Poziom ET-1 mierzono testem ELISA. Stwierdzono statystycznie istotną różnicę poziomu ET-1 pomiędzy badanymi grupami, który wynosił, odpowiednio, 33,00 ń 12,07 vs. 25,00 ń 5,69 pg/mL (p = 0,005). Z obserwacji powyższej wynika, że podwyższony poziom endoteliny u kobiet ciężarnych mógłby być wczesnym markeremm nadciśnienia indukowanego ciążą.

Streszczenie angielskie: Pregnancy induced hypertension (PIH) is accompanied by injury and further activation of placental endothelial cells. Activated endothelial cells produce several mediators, among them endothelin-1 (ET-1) - one of the most potent vasoconstrictors. The aim of the study was to examine the ET-1 level in serum of 18 women with PIH and compare it to the group of 16 normotensive pregnant women. ET-1 level, as evaluated by ELISA test, was significantly higher in PIH than in normotensive pregnancy, 33.00 ń 12.07 vs. 25.00 ń 5.69 pg/mL (p = 0.005), respectively. It might be concluded, that ET-1 level is a prognostic parameter, indicating the possibility of PIH development.

661/2523

Tytuł oryginału: Integrated minimally invasive direct coronary artery bypass grafting and angioplasty for coronary artery revascularization.
Autorzy: Cisowski Marek, Morawski Włodzimierz, Drzewiecki Janusz, Kruczak Wojciech, Toczek Krzysztof, Bis Jarosław, Bochenek Andrzej
Źródło: Eur. J. Cardiothorac. Surg. 2002: 22 (2) s.261-265, tab., bibliogr. 20 poz.
Sygnatura GBL: 312,899

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Objective: Minimally invasive direct coronary artery bypass (MIDCAB) through the anterolateral minithoractomy has become a promising therapeutic option in patients with lesion in left anterior descending artery (LAD), especially in multimorbid, elderly and reoperated patients with type C or B lesions. To expand the benefits of MIDCAB concept to patients with multivessel disease, a hybrid myocardial revascularization procedure (HMR) combining surgery of the LAD with interventional proedures for additional coronary lesions has recently been introduced. Methods: Between January 1999 and September 2001, 50 patients (37 male, 13 female, mean age 54.8 ń 20.1 years) underwent an HMR procedure. MIDCAB with endoscopic left internal thoracic artery (LITA) harvesting, followed by percutaneous coronary intervention (PCI) for additional coronary lesions and percutaneous transluminal coronary angioplasty (PTCA), was performed in 11 patients (22 p.c.) and stenting in 39 patients (78 p.c.). Angiographic assessment of graft patency was performed in all patients during the PCI procedure. The clinical follow-up period was 3-32 months. Results: There were no early and late deaths. Baseline Canadian Cardiology Society (CCS) class was 2.8 ń 0.7 versus 1.1 ń 0.9 (P 0.001) 30 days after HMR procedure. There were no major acute inhospital cardiac events. Angiographic studies showed patent LIMA-LAD graft in 50 patients (100 p.c.). We showed quality of anastomosis in 49 patients (98 p.c.). There was a moderate graft stenosis in one ...

662/2523

Tytuł oryginału: Soluble adhesion molecules (ICAM-1, VCAM-1, and E-selectin) and vascular endothelial growth factor (VEGF) in patients with distinct variants of rheumatoid synovitis.
Autorzy: Klimiuk P. A., Sierakowski S., Latosiewicz R., Cylwik J. P., Cylwik B., Skowroński J., Chwiecko J.
Źródło: Ann. Rheum. Dis. 2002: 61 (9) s.804-809, il., tab., bibliogr. 46 poz.
Sygnatura GBL: 310,264

Hasła klasyfikacyjne GBL:

reumatologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Cell adhesion molecules and endothelial growth factors have an important role in the infiltrating of rheumatoid synovium with mononuclear cells, leading to the initiation and progression of the disease. Objective: To investigate whether the serum profile of soluble adhesion molecules and of vascular endothelial growth factor (VEGF) is associated with the histological apperance of rheumatoid arthritis (RA). Methods; Serum levels of soluble intercellular adhesion molecule-1 (slCAM-1), vascular cell adhesion molecule-1 (sVCAM-1), E-selectin (sE-selectin), and VEGF were assessed by enzyme linked immunosorbent assay (ELISA) in 40 patients with RA and 32 patients with osteoarthritis (OA). Results: Histological analysis of synovium specimens distinguished two types of rheumatoid synovitis. Twenty four RA samples presented diffuse infiltrates of mononuclear cells without any further microanatomical organisation, whereas in the remaining 16 samples lymphocytic follicles with germinal centrelike structures were identified. In comparison with patients with OA, constituting a control group, higher serum concentrations of slCAM-1 (p 0.001), sE-selectin (p,0.01), and VEGF (p0.001) were detected in patients with RA. Raised concentrations of slCAM-1, and VEGF dominated in the serum of patients with RA with follicular synovitis compared with those with diffuse synovitis (p 0.01 for all comparisons). The serum concentrations of slCAM-1, sVCAM-1, and VEGF correlated with markers of disease activity such as the erytchrocyte sedimentation rate and C reactive ...

663/2523

Tytuł oryginału: Genetics of human essential hypertension.
Autorzy: Jeunemaitre Xavier
Źródło: Kardiol. Pol. 2002: 56 supl. 1: 5 Sympozjum Sekcji Kardiologii Doświadczalnej Polskiego Towarzystwa Kardiologicznego "Nadciśnienie tętnicze" s.I-47 - I-50, bibliogr. 25 poz. - 5 Sympozjum Sekcji Kardiologii Doświadczalnej Polskiego Towarzystwa Kardiologicznego pt. Nadciśnienie tętnicze Konstancin 12. 2000
Sygnatura GBL: 313,397

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

664/2523

Tytuł oryginału: Contact guidance of Walker carcinosarcoma cells by the underlying normal fibroblasts is inhibited by RGD-containing synthetic peptides.
Autorzy: Madeja Zbigniew, Sroka Jolanta
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.251-260, il., tab., bibliogr. 35 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The metastatic spread of malignant neoplasms is associated with active migration of cancer cells. The migration of neoplastic cells during the metastatic process may be affected by various extracellular factors, including chemoattractants, haptotactic signals, electric fields, substrate anisotropy, and cell-to-cell contacts. We examined the effect of homotypic collisions and heterotypic interactions with normal human skin fibroblast on the motile activity of Walker carcinosarcoma cells. It was found that Walker carcinosarcoma cells moving in a dense population neither show contact inhibition of movement when colliding with one another nor increase their motile activity as a result of contact stimulation of motility. On the other hand, when plated onto the surface of aligned fibroblasts, Walker carcinosarcoma cells migrated mainly along the long axes of underlying fibroblasts as a result of contact guidance. The directional character of movement (but not the speed of migration) of Walker carcinosarcoma cells on the surface of aligned fibroblasts was completely effaced by RGD-containing synthetic peptide at a concentration of 1 mg/ml but not by 5 ćM verapamil (selective voltage-gated calcium channel inhibitor) or 10 ćM gadolinium chloride (non-specific blocker of mechanosensitive ion channels). The suppression of directional character of migration of tumour cells by RGD-containign peptide was associated with the decrease in the amount of fibronectin macromolecules attached to firbroblasts. This suggests that alignment and anisotropic distribution of fibronectin macromolecules may be responsible for contact guidance of tumour cells moving on the surface of fibroblast.

665/2523

Tytuł oryginału: Folia Histochemica et Cytobiologica
Tytuł polski: Characterization of afferent and efferent galanin-containing nerve fibres in the porcine ovary.
Autorzy: Majewski Mariusz, Kaleczyc Jerzy, Wąsowicz Krzysztof, Bossowska Agnieszka, Gonkowski Sławomir, Klimaschewski Lars
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.261-268, il., tab., bibliogr. 42 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

weterynaria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnie

Streszczenie angielskie: Double-lebelling immunofluorescence combined with retrograde tracing revealed three subpopulations of galaninimmunoreactive nerve fibres innervating the porcine ovary. The first group originated in ipsilateral dorsal root ganglia Th11-L5 and exhibited colocalization with substance P-, but not with tyrosine-hydroxylase- or dopamine-á-hydroxylase-immunoreactivity. These fibres supplied primordial follicles and large arteries of the ovarian hilus. The second group, arising from the sympathetic inferior mesenteric ganglion, was galanin-immunoreactive, but non-reactive to substance P-, dopamine-á-hydroxylase- or tyrosine-hydroxylase-antisera. Fibres of this type were sparsely distributed in the ovarian cortex. They were observed in connection with small cortical arterioles or in the ovarian cortical stroma. The third group of galanin-containing nerve terminals originated from postganglionic neurons located in sympathetic chain ganglia Th15-L3 and S1. Most of them contained tyrosine-hydroxylase- and dopamine-á-hydroxylase-immunoreactivity. This subset of galanin-positive nerve fibres was the least frequent and was almost exclusively associated with cortical arterioles. The findings are indicative of an involvement of galanin in autonomic and sensory neuronal functions in the ovaries, possibly control of follicle maturation and blood flow.

666/2523

Tytuł oryginału: Effect of exogenous pulmonary surfactant preparations on the structure of pulmonary alveoli in newborn rats.
Autorzy: Żurawski Jakub, Wąsowicz Marcin, Marszałek Andrzej, Florek Ewa, Biczysko Wiesława
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.285-295, il., bibliogr. 40 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Treatment of pre-term newborns with exogenous surfactant preparation is a well established part of the therapy for respiratory distress syndrome of the newborns (RDS). Since the introduction of surfactant into clinical practice in 1980, hundreds of studies have been published describing beneficial effects of such treatment. There is only limited number of morphological publications reporting adverse effects of surfactant administration. The aim of the present study is to describe morphological changes in the lung after surfactant administration to healthy newborn rats. Two types of surfactant were used: Exosurf (Glaxo Wellcome, England) and Survanta (Abbott Laboratories, USA). Surfactant preparation were given intratracheally in single dose (bolus) (100 mg of lipids per kg b.w.). Animals from control group received 0.9 p.c. saline in equivalent volume. Lung specimens were taken 15, 20, 25 and 30 minutes after drug administration and evaluated by light and electron microscopy. There was no damage in lungs from the control group. Tissue specimens from the Exosurf group revealed severe pathological changes: foci of atelectasis, frank edema in the parenchyma, focal disruption of air-blood barrier, hemorrhages in many alveoli, surfactant particles in man alveolar capillaries, and strongly activated alveolar macrophages. In this group changes appeared as early as 15 min after surfactant administration and intensity of lung injury increased with time. Also, Survanta administration caused damage to the lung tissue. However, the changes were less intense and appeared later (20 - 25 minutes after Survanta treatment). In conclusion, the presented morphological findings proved that exogenous surfactant administration to healthy rat nevborns caused lung damage. Comparing two different surfactant preparation. Exosurf and Survanta, it was shown that the former one produced stronger and faster damage to lung alveoli than the latter one.

667/2523

Tytuł oryginału: Comparison of the cell immunophenotype of metastatic and primary foci in stage IV-S neuroblastoma.
Autorzy: Nowicki Michał, Miśkowiak Bogdan
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.297-303, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: Neuroblastoma represents one of the most frequently developing malignant solid tumours in children. At the time of diagnosis, in more than half of the bone marrow. The present study was aimed at immnocytochemical analysis of selected neuropeptide manifestation in metastatic cells of neuroblastoma in bone marrow and at comparing the obtained results with the immunophenotype of parental neuroblastoma cells. The studies were performed on bone marrow material obtained from children treated at the Department of Paediatric Haematology and Oncology, University of Medical Sciences, Poznań, Poland, in 1998-2000. Immunocytochemical analysis of nervous tissue markers (employing the immunomax technique) involved 36 bone marrow preparations obtained from 27 children. The analysis included espression of neuron-specific enolase (NSE), PGP 9.5 protein, substance P (SP), chromogranin A (ChA), bombesin (B), galanin (G), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). Close to 90 p.c. metastatic cells in bone marrow were found to exhibit NSE+SP+B+ phenotype and over a half of the cells manifested additionally expression of PGP 9.5+ChA+NPY+. Comparison of the obtained results with the immunophenotype of neuroblastoma cells obtained directly from the primary tumour demonstrated high correlationof NSE, SP and PGP 9.5 expression. Due to the relative ease of obtaining the bone marrow material and absence of neuromarkers in boen marrow metastatic cells in solid tumors other than neuroblastoma, determination of immunophenotype of the cells may represent a valuable supplementation in preliminary diagnosis of this tumour in children.

668/2523

Tytuł oryginału: Expression of class III á-tubulin in neuroendocrine tumours of gastrointestinal tract.
Autorzy: Jirasek Tomas, Mandys Vaclav, Viklicky Vladimir
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.305-309, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Class III á-tubulin is regarded as a specific marker for the cells of neuronal origin as well as for the tumours originating from these cells. Its expression is considered one of the earliest events that appear in the cells revealing neuronal differentiation. Using monoclonal antibody TU-20 in an immunohistochemical analysis, we studied the expression of class III á-tubulin in gastrointestinal carcinoid tumours. Paraffin-embedded, formalin-fixed tissue sections from 49 tumour samples obtained from following locations: stomach (4 cases), small intestine (8 cases), appendix (18 cases), rectum (3 cases), pancreas (5 cases), liver metastases (7 cases) and lymph node metastases (4 cases) were used in the study. In 41 of the 49 tumour samples (83.7 p.c.), positive staining for class III á-tubulin was detected, while 8 tumour samples (16.3 p.c.) were negative. Expression of class III á-tubulin was prominent in all three rectal carcinoids and in three atypical caracinoids located in small intestine. Pancreatic neuroendocrine tumours revealed either weak immunostaining (2 cases), or were negative for this marker (3 cases). The intensity of class III á-tubulin immunolabelling was not related to the degree of tumour differentiation. The results of this study suggest that class III á-tubulin could be a perspective marker for gastrointestinal neuroendocrine tumours. Moreover, the differences in its expression could also elucidate some aspects of histogenetic relationships of neuroencodcrine tumours of gastrointestinal tract.

669/2523

Tytuł oryginału: Egg yolk platelet proteins from "Xenopus laevis" are amyloidogenic.
Autorzy: Zemanek Grzegorz, Konieczny Leszek, Piekarska Barbara, Rybarska Janina, Stopa Barbara, Spólnik Paweł, Urbanowicz Barbara, Nowak Mateusz, Król Marcin, Roterman Irena
Źródło: Folia Histochem. Cytobiol. 2002: 40 (3) s.311-318, il., bibliogr. 39 poz.
Sygnatura GBL: 304,846

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The association of amphibian (Xenopus laevis) egg yolk platelet proteins, represented predominantly by lipovitellin, was studied as a model of the formation of amyloid deposits. Two kinds of molecular organization formed by this protein material - native and heat-denatured - were found to exhibit amyloid properties, although they differ significantly in structural organization. The first consisted in protein molecules arranged in the natural, physiological, net-like platelet organization, with a tendency to orient uni-directionally. The second was obtained by the gradual removal of Congo red from lipovitellin denatured by heating in an excess of dye. This procedure produced twisted fibrillar organization of molecules typical for amyloids, represented predodminantly by end-to-end associated major polypeptide chains of lipovitallin. Both native and denatured structural forms bind Congo red and produce a green birefringence effect, confirming the near-parallel alignment of the complexed Congo red molecules. However, a dye (1,4-bis(1-amino-4-sulfonaphtyl-2-azo)phenylene) closely related to Congo red but with a very weak self-assembling tendency appeared inactive when the spectral shift was studied in a cross-polarization systsem, indicating in this way that dye supramolecularity is an extra factor which may determine binding to amyloid proteins and specific spectral effects.

670/2523

Tytuł oryginału: Determination of the content of desmopressin in pharmaceutical preparations by HPLC and validation of the method.
Autorzy: Dudkiewicz-Wilczyńska Jadwiga, Snycerski Andrzej, Tautt Jadwiga
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.163-168, il., tab., bibliogr. 13 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The aim of this study was to apply high performance liquid chromatography to the determination of content of desmopressin in pharmaceutical preparations and validation of the method. The satisfactory results have been obtained using a column Luna C 8.5 ćm, 100 x 4.6 mm and a mobile phase containing 0.067 M phosphate buffer of pH = 7 and acetonitrile in the proportion 83 : 17. It has been shown that the elaborated method shows good precision and accuracy and can be applied to the qualitative and quantitative analysis of pharmaceutical preparations containing desmopressin.

671/2523

Tytuł oryginału: Acidimetric titration of medicines being salts of weak acids and determining the end-point based on the iodate(V)-iodide reaction.
Autorzy: Somogyi Eugeniusz, Piotrowska Joanna
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.169-170, tab., bibliogr. 1 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A sample being a salt of weak acid in a solvent is determined with hydrochloric acid in the presence of iodate, iodide, and starch. The excess acid colours the solution blue.

672/2523

Tytuł oryginału: Naproxen ion-selective electrode and its application to pharmaceutical analysis.
Autorzy: Lenik Joanna, Dumkiewicz Ryszard, Wardak Cecylia, Marczewska Barbara
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.171-176, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: An ion-selective membrane electrode was prepared based on ion-pair complex of naproxen with methyltrioctylammonium. Its basic analytical parameters such as: slope characteristics, measuring range, detection limit, response time were determined. The electrode showed Nernstian response from the 10**-1 to 10**-4 mol 1*-1 concentration range and 5.5 - 8.5 pH range, low limit of detection 5 x 10**-5 mol 1*-1 and short response time -20s. Selectivity was good over a number of organic and inorganic anions. The electrode was applied for the determination of naproxen tablets in aqueous solutions by the calibration curve method and standard addition method.

673/2523

Tytuł oryginału: Saline cathartics and adsorptive capacity of activated charcoal for doxycycline.
Autorzy: Afonne O. J., Orisakwe O. E., Ofuefule S. I., Tsalha S., Obi E., Ilondu N. A., Okorie O.
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.177-179, il., bibliogr. 8 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The effects of sodium chloride and sodium citrate on the in vitro adsorption of doxycycline to activated charcoal have been studied. Solutions of doxycycline alone and doxycycline with 7.5 mg/ml cathartic solutions were vortex - mixed for 30 s with different quantities of activated charcoal, incubated for 30 min at 37řC and analyzed for free doxycycline spectrophotometrically at 348 nm. Addition of NaCl had a significant (p 0.05) increase while sodium citrate produced a significant (p 0.05) decrease in the adsorption of doxycycline on activated charcoal. In all, the adsorption doxycycline on activated charcoal obeyed quantity - dependent kinetics.

674/2523

Tytuł oryginału: Biopharmaceutical assessment of eye drops containing aloe (Aloe arborescens Mill.) and neomycin sulphate.
Autorzy: Kodym Anna, Grześkowiak Edmund, Partyka Danuta, Marcinkowski Andrzej, Kaczyńska-Dyba Ewelina
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.181-186, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The subject of the studies was eye drops made of aloe, containing the group of aloe chemical substances of anti-inflammatory use and neomycin sulphate. The aim of the studies was to evaluate the permeability of biologically active aloe substances, determined as aloenin, through synthetic lipophilic and hydrophilic membranes in a standard perfussion apparatus and in vitro verification of the transport possibilities of these substances through the isolated cornea of pig's eye. The permeability process of biologically active aloe substances determined as aloenin, through synthetic lipophilic and hydrophilic membranes, was analyzed using the first-order kinetics. Estimated quotas of permeability rate constant show that the investigated chemical compounds of aloe, included in the eye drops, diffused through the applied membranes. The studies of permeability through isolated pig's cornea proved that biologically active aloe substances could not overcome this biological larrier. On the basis of biopharmaceutical studies it can be concluded that the eye drops containing aloe and neomycin sulphate, due to the lack of permeating abilities through the eye cornea, should be particularly useful in the treatment of inflammations and infections of external parts of the eye, such as conjuctiva, eyelid edges, lacrimal sac and cornea.

675/2523

Tytuł oryginału: The effect of cryoprotectants on the physical properties of large liposomes containing sodium diclofenac.
Autorzy: Janicki Stanisław, Jankowski Jacek, Szulc Janina, Woyczikowski Brunon, Sznitowska Małgorzata
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.187-191, il., tab., bibliogr. 6 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Large liposomes (1-10 ćm) containing sodium diclofenac were prepared and lyophilized using lactose or mannitol (7.5 p.c. in respect to the lipid content) as cryoprotectants. The physical studies of liposomes were performed during 30 days of storage in a dry or resuspended form. Lyophilization of large liposomes and storage in the dry form at 5řC increases their physical stability. Lactose is a cryoprotectant which does not influence changes of properties of liposomes regarding their size, encapsulation efficacy and release rate. Large liposomes lyophilized in the presence of mannitol tend to increase in size and encapsulation efficacy, but the lipid bilayers are stabilized and less permeable to the drug.

676/2523

Tytuł oryginału: Mechanism of in vitro release kinetics of flurbiprofen loaded ethylcellulose micropellets.
Autorzy: Mallick Subrata, Roy Kunal, Chakraborty Atanu, Saha Subhayu
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.193-198, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Flurbiprofen loaded ethylcellulose micropellets with different drug loading were prepared by a quasi-emulsion solvent diffusion technique. Encapsulation parameters of micropellets such as actual drug loading, drug encapsulation efficiency (DEE) and loss of coating polymer (LCP) were determined. Actual drug loading was increased with the increased initial drug loading whereas encapsulation efficiency decreased with the increase of actual drug loading. In vitro drug release profiles of these micropellets were evaluated in distilled water (DW) and also in phosphate buffer solution (PBS) to indicate pH dependency release rates. All the batches of micropellets released about 35-59 p.c. in DW and 89-97 p.c. in PBS during the period of 8 h and the burst effect of about 50-75 p.c. in the first 1.5 h was seen only in PBS. The mechanism of release kinetics was evaluated by fitting the release data to the zero order, first order. Higuchi, Baker-Lonsdale and Peppas equations and also to the differential forms of zero order, first order and Higuchi model. Adequate fitting of release data was found with first order, Higuchi and Peppas models and hence these models were selected for F-test statistics for ascertaining the mechanism of drug release. Higuchi model of drug release in DW an dPBS of all the formulations was ruled out due to its significantly different F-value with other models. Thus, mechanism of release of flurbiprofen from ethylcellulose micropellets may be explained by the diffusional exponent model of Peppas et al. as ascertained by F-test statistics rather than the same, based on some other diffusional models even though they have shown good correlation.

677/2523

Tytuł oryginału: Synthesis of 6-substituted 6H-indolo[2,3-b]quinolines as novel cytotoxic agents and topoisomerase II inhibitors.
Autorzy: Kaczmarek Łukasz, Łuniewski Wojciech, Zagrodzki Bogdan, Godlewska Joanna, Osiadacz Jarosław, Wietrzyk Joanna, Opolski Adam, Peczyńska-Czoch Wanda
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.199-207, il., tab., bibliogr. 8 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: A systematic investigation into the impact of the substituents introduced into the indolo[2,3-b]quinoline system is described. The findings clearly demonstarte that the compounds bearing a methyl group or a longer aliphatic chain at the N-6 position are inactive against prokaryotic and eukaryotic cells. The indroduction of alkyl-amino-alkyl substituent at the N-6 position of indolo[2,3-b]quinoline accounts for the appearance of the antimicrobial and cytotoxic properties. The cytotoxicity against oral epidermoid carcinoma KB(ID50) is in the range from 2.0 to 9.0 ćM, and the antimicrobial activity (MIC) falls between 0.003 and 0.50 mM. The structural relation within 6H-indolo[2,3-b]quinolines, concering their antimicrobial and cytotoxic activity, corresponds well with their ability to bind DNA and to inhibit topoisomerase II activity.

678/2523

Tytuł oryginału: Synthesis and anticonvulsant properties of new 1-phenyl and 1-phenyloamino-3-penylpyrrolidine-2,5-dione derivatives.
Autorzy: Obniska Jolanta, Zejc Alfred, Zagórska Agnieszka
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.209-213, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: A series of N-aryl and N-aminoaryl 3-phenyl pyrrolidine-2,5-diones were synthesized and tested for anticonvulsant activity in the maximum electroshock seizure (MES) and pentetrazol seizure threshold (sc Met) tests. Structures of the novel compounds were confirmed by elemental and spectral analyses.

679/2523

Tytuł oryginału: Synthesis and antiproliferative activity in vitro of new 3-substituted aminopyrazolo[3,4-b]pyridines.
Autorzy: Poręba Krystyna, Opolski Adam, Wietrzyk Joanna
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.215-222, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The synthesis of several new 3-substituted aminopyrazolo[3,4-b]pyridines is described. The obtained compounds were tested for their antiproliferative activity in vitro. Two of them: 3-chloroacetylaminopyrazolo[3,4-b]pyridine [II] and 3-(2-bromopropionyl-amino)pyrazolo[3,4-b]pyridine [III] revealed cytotoxic activity against the cells of 5 human tumor cell lines applied. Their ID50 values were in the range of the international activity criterion for synthetic agents (4 ćg/ml). The structures of the products II - XVII were estabilished on the basis of elemental analysis and spectral data (IR, 1H NMR and MS).

680/2523

Tytuł oryginału: Synthesis and antimicrobial activity of 3-hydroxyimino-5-methyl-2-hexanone(HIMH) and its dioxime derivative.
Autorzy: Patil Vishwanath R., Donde Kamini J., Jadhav Sanjeev B., Malve Sheela P.
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.223-225, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A new oxime, 3-hydroximino-5-methyl-2-hexanone (HIMH) has been synthesized by the reaction of 1-pentyl nitrite with 5-methyl-2-hexanone under acidic conditions. The subsequent treatment of HIMH with NH2OH HCL gives 5-methyl-2,3-hexanedione dioxime (H2MHDDO). The structures of these compounds have been confirmed by physicochemical and spectral data. A preliminary screening of thses compounds for biological activity against several microorganisms has indicated that they are selective growth inhibitors of m-tuberculosis, in particular.

681/2523

Tytuł oryginału: Flavonoid compounds in the flowers of Abutilon indicum (L.) Sweet (Malvaceae).
Autorzy: Matławska Irena, Sikorska Maria
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.227-229, bibliogr. 15 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Seven flavonoid compounds: luteolin, chrysoeriol, luteoin 7-O-á-glucopyranoside, chrysoeriol 7-O-á-glucopyranoside, apigenin 7-O-á-glucopyranoside, quercetin 3-O-á-glucopyranoside, quercetin 3-O-ŕ-rhamnopyranosyl (1 - 6)-á-glucopyranoside, were isolated and identified from the flowers of Abutilon indicum (L.) Sweet (Malvaceae).

682/2523

Tytuł oryginału: Evaluation of probucol as suppressor of ceftizoxime induced lipid peroxidation.
Autorzy: Roy Kunal, Saha Achintya, De Kakali, Sengupta Chandana
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.231-234, tab., bibliogr. 34 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Considering drug induced lipid peroxidation, a possible mediator of drug induced toxicity and exploiting free radical scavenging action of probucol, which is a synthetic antioxidant of therapeutic interest, in vitro effects of the antioxidant on drug induced lipid peroxidation have been studied to explore its possible potential in reducing drug induced toxicity. In the present study, ceftizoxime sodium, a third generation of cephalosporin, has been taken as the representative drug and goat whole blood has been used as the lipid source. The study revealed that probucol could suppress drug induced lipid peroxidation to a significant extent. This provides scope for further study on probucol to evaluate its potential for reducing drug induced toxicity and increasing therapeutic index of drug by possible cotherapy.

683/2523

Tytuł oryginału: Synthesis of new derivatives of 3-methyl-8-aryl-7,8-dihydro-6H-imidazo[2,1-c][1,2,4] triazin-4-one.
Autorzy: Sztanke Krzysztof
Źródło: Acta Pol. Pharm. 2002: 59 (3) s.235-236, tab., bibliogr. 12 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Cyclocondensation of 1-aryl-2-hydrazinoimidazolines with pyruvic acid furnished novel derivatives of imidazo [2,1-c] [1,2,4]triazin-4-one.

684/2523

Tytuł oryginału: Disturbed right ventricular ejection pattern as a new Doppler echocardiographic sign of acute pulmonary embolism.
Autorzy: Kurzyna Marcin, Torbicki Adam, Pruszczyk Piotr, Burakowska Barbara, Fijałkowska Anna, Kober Jarosław, Oniszh Karina, Kuca Paweł, Tomkowski Witold, Burakowski Janusz, Wawrzyńska Liliana
Źródło: Am. J. Cardiol. 2002: 90 (5) s.507-511, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 304,619

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Transthoracic echokardiography (TTE) is frequently performed in patients with suspected acute pulmonary embolism (APE) to search for right ventricular (RV) pressure overload. We prospectively assessed the diagnostic value on the disturbed RV ejection pattern ("60/60 sign") and compared its diagnostic performances with that of the presence of RV pressure overload, as well as with "McConnell sign" based on RV regional wall motion abnormalities. We assessed 100 consecutive patients with clinical suspicion of APE, including those with previous cardiorespiratory disease. After TTE, all of the patients underwent reference diagnostic tests for APE. The 60/60 sign required RV occeleration time of ó 60 ms in presence of tricuspid insufficiency pressure gradient ó 60 mm Hg. APE was ultimately confirmed in 67 of 100 patients. In 17 of 67 patients, 60/60 sign correctly suggested APE, whereas 2 false-positive results were noted: in pulmonary fibrosis and in acute respiratory distress syndrome following lung resection. McConnell sign was fully specific but was noted in only 13 of 67 patients. Sensitivity and specificity were 81 p.c. and 45 p.c. for "RV-pressure overload" signs, 25 p.c. and 94 p.c. for 60/60 sign, and 19 p.c. and 100 p.c. for McConnell sign. When combined, the 2 latter signs were 94 p.c. specific and 36 p.c. sensitive in diagnosing APE. Thus, RV overload at echocardiography is not specific for APE. The 60/60 and McConnell signs are insensitive, but are reliabe and helpful in bedside diagnosis of APE when direct visualization of the pulmonary arteries is impossible...

685/2523

Tytuł oryginału: Intravascular ultrasonic evaluation of the magnitude of stent expansion and the mechanisms of lumen enlargement after direct stenting and after conventional stenting with balloon predilatation.
Autorzy: Dudek Dariusz, Legutko Jacek, Kałuża Grzegorz L., Żmudka Krzysztof, Dubiel Jacek S.
Źródło: Am. J. Cardiol. 2002: 90 (6) s.639-641, il., tab., bibliogr. 9 poz.
Sygnatura GBL: 304,619

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Currently, a growing number of stents are deployed in a coronary artery without, a preceding ballon dilation (direct stenting [DS]). The mechanisms of lumen enlargement after conventional stent implantation have been recently studied by intravascular ultrasound (IVUS) and encompass: (1) vessel expansion, (2) axial redistribution of plaque, and (3) plaque compression or embolization. In contrast, the impact of direct stent implantation on the plaque and vessel segment undergoing intervention has not been charcterized. The present study investigates the mechanisms of lumen enlargement after coronary DS.

686/2523

Tytuł oryginału: Tissue-specific association of the human tyrosine hydroxylase gene with the nuclear matrix.
Autorzy: Lenartowski Robert, Goc Anna
Źródło: Neurosci. Lett. 2002: 330 (2) s.151-154, il., bibliogr. 20 poz.
Sygnatura GBL: 305,936

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Association of the human tyrosine hydroxylase (TH) gene with the nuclear matrix was studied using bovine matrices from tissues differing in expression of the gene. An in vitro binding assay was performed with fragments encompassing the -2300/+2300 region of the human TH gene. All fragments bound to the nuclear matrix from the adrenal medulla but none bound to that from the liver. A computer search of the sequenced part of the studied region revealed putative matrix attachment regions (S/MAR) - one in the promoter and two in the first intron. The (TCAT)n microsatellite repeat included in one the intronic S/MARs seems to influence the binding potential of the human TH gene to the nuclear matrices.

687/2523

Tytuł oryginału: Comparative studies on the occurrence of protozoans, bacteria and fungi in the oral cavity of patients with systemic disorders.
Autorzy: Chomicz Lidia, Piekarczyk Janusz, Starościak Bohdan, Fiedor Piotr, Piekarczyk Barbara, Szubińska Dorota, Zawadzki Paweł J., Walski Michał
Źródło: Acta Parasitol. 2002: 47 (2) s.147-153, il., bibliogr. s. 152-153
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of this study was to compare the oral cavity status with the species composition of microorganisms colonizing the mounth of patients with or without systemic diseases. Seventy-seven men and women, 19 to 65-years-old, of four groups: hemodialyzed patients (HD), kidney allograft recipients (Tx), congenitally disordered (Cd) and control (C) patients (without systemic diseases) were examined clinically for the oral cavity status and the microorganisms occurring in the mounth were identified. Swabs and samples taken from dental plaques, pockets and periodontium were used for light and electron microscopical studies as well as for protozoan, bacterial and fungal cultures. Entamoeba gingivalis and Trichomonas tenax were found in the HD, Tx, Cd and C groups (top prevalence 14, 14, 87.5 and 25 p.c. respectively); free-living amoebae (with some features of Acanthamoeba sp.) also occurred in several cases. Staphylococcus aureus bacteria were found in 66 p.c. of Cd patients infected with the protozoans. Numerous fecal bacteria and/or fungi Candida albicans have been observed in 41-51-year-old Cd and HD patients. In all patient groups, it was the 41-51-year-olds who showed the highest prevalence of protozoans, bacteria and fungi. The results indicate that metabolic disabilities favour pathological changes in periodontal tissues and may influence the species composition of mixed protozoan, bacterial and/or fungal infections in various ways, in patients with different systemic diseases.

688/2523

Tytuł oryginału: The red fox, Vulpes vulpes L., as a potential resorvoir of zoonotic flukes in the Iberian Peninsula.
Autorzy: Segovia Juan Matias, Torres Jordi, Miquel Jordi
Źródło: Acta Parasitol. 2002: 47 (2) s.163-166, il., tab., bibliogr. [21] poz.
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

weterynaria

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: A parasitological suvery of 396 red foxes (Vulpes vulpes L.) from the Principat d'Andorra, 34 Spanish provinces and Serra da Malcata (Portugal) was carried out to evaluate the fluke status of this wild canid in the Iberian Peninsula. Special attention was devoted to the epidemiological role of this canid in maintaining the potential zoonotic distomatosis. Four fluke species were detected: adults of Brachylaima sp., Alaria alata, Opisthorchis felineus and Metorchis bilis. Seventeen foxes (4.3 p.c.) were infected by at least one of these digeneans. All flukes with an aquatic life cycle were found mainly (26.9 p.c.) in a delimited zone (called zone A), characterised by high amount surface water, with A. alata being the most prevalent species (19.2 p.c.) . Metorchis bilis is more frequently and widely distributed throughout Iberia than O. felineus. Since both flukes have a very similar life cycle, this might be the result of a distinct distribution pattern of their appropriate snails. This study shows that a relatively large natural focus of potential zoonotic flukes (zone A) is located in central Iberia, near the boundary of Portugal with Spain.

689/2523

Tytuł oryginału: PFC modular total knee replacement system - middle term results.
Tytuł polski: Modularny system do całkowitej aloplastyki stawu kolanowego PFC - wyniki w średnim okresie.
Autorzy: Janecek M., Bucek P.
Źródło: Ortop. Traumatol. Rehabil. 2002: 4 (3) s.360-365, il., tab., bibliogr. 18 poz., streszcz.
Sygnatura GBL: 313,531

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wstęp. Całkowita aloplastyka stawu kolanowego jest obecnie preferowanym rozwiązaniem w przypdkach ciężkich zmian zwyrodnieniowych, zmian w przebiegu reumatoidalnego zapalenia stawów lub w przypadku pourazowych zmian zwyrodnieniowych. Jest ona wskazana u pacjentów w różnym wieku, z wieloma rodzajami zmian patologicznych. Każdy ze stawów wymaga indywidualnego postępowania. Materiał i metody. Autorzy przeprowadzili badania prospektywne 500 pierwotnych aloplastyk całkowitych stawu kolanowego z użyciem protezy modularnej PFC. W latach 1991-2001 przeprowadzono implantację 350 andoprotez (55 obustronnie) u 295 pacjentów z rozpoznaniem pierwotnych zmian zwyrodnieniowych stawu kolanowego, reumatoidalnego zapalenia stawu kolanowego lub zmianach zwyrodnieniowych kolana pourazowych. Średni wiek w badanej grupie pacjentów wynosił 68,3 lata (2883). Badaniem objęto 201 kobiet i 94 mężczyzn. Średni okres obserwacyjny wynosił 7,8 roku (od 2,2 do 10,4 lat). Krzywą przetrwania protezy skonstruowano w oparciu o liczbę zabiegów rewizyjnych z powodu obluzowań aseptycznych. Wyniki. Zgodnie z Systemem Oceny Klinicznej Towarzystwa Kolana, wyniki bardzo dobre uzyskano w 288 kolanach, dobre w 45, złe w 13 i bardzo złe w 4. Obserwowano tylne zwichnięcie komponenty piszczelowej w 2 przypadkach. U jednego pacjenta konieczne było przeprowadzenie wymiany implantu podczas procedury dwuetapowej z powodu obluzowania septycznego. Wnioski. System całkowitej endoprotezy stawu kolanowego PFC wydaje się być protezą dobrej jakości, pozwalającą na rozwiązanie wszystkich problemów mogących powstać w przypadkach aloplastyk pierwotnych i rewizyjnych. Uzyskano dobre wyniki leczenia i wysoki odsetek wyników satysfakcjonujących pacjentów.

Streszczenie angielskie: Bacgriund. Total knee replacement, which is now the preferred solution for severe primary osteoarthritis, rheumatoid arthritis or posttraumatic osteoarthritis, is indicated in patients of various ages with various pathological changes and deformities. Every joint requires an individual treatment. Material and method. We performed a prospective study of 500 cemented primary PFC modular total knee replacement implanted from 1991 to 2001, involving 350 prosthesis (55 bilateral) in 295 patients wirh diagnosis of primary osteoarthriris, rheumatoid arthritis or posttraumatic osteoarthritis. The average age of our patients was 68.3 years (range 28-83). The study sample included 201 women and 94 men. The mean follow up was 7.8 years (range 2.2 - 10.4 years). A survivorship curve was constructed based on revision surgery due to aseptic loosening. Results. According to the Knee Society Clinical Rating System, the results were excellent in 288 knees, good in 45 knees, poor in 13 knees and extremely poor in 4 knees. We observer posterior dislocation of the tibial component in 2 cases. In one patient we had to replace in a two-stage procedure because of septic loosening. Conclusions. The PFC total knee system seems to be a prosthesis of good quality, permitting the solution of almost any problem that may arise in both primary and revision cases. We achieved good outcomes and high patient satisfaction.

690/2523

Tytuł oryginału: Impact of Helicobacter pylori and nonsteroidal anti-inflammatory drugs on gastric ulcerogenesis in experimental animals and in humans.
Autorzy: Pawlik Teresa, Konturek Peter C., Konturek Jan W., Konturek Stanisław J., Brzozowski Tomasz, Cześnikiewicz Marta, Płonka Małgorzata, Bielański Władysław, Areny Hany
Źródło: Eur. J. Pharmacol. 2002: 449 (1/2) s.1-15, il., bibliogr. s. 13-15
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Helicobacter pylori (H. pylori) and nonsteroid anti-flammatory drugs (NSAID) are the most common pathogens in the gastroduodenal mucosa in animals and humans, but their relationship in ulcerogenesis has been little studied. Acording to some authors, H. pylori infections in humans does not act synergistically with NSAID on ulcer healing, therefore, there is no need to eradicate the germ. This notion is supported by the finding that the eradication of H. pylori does not affect NSAID-induced gastropatyhy treated with omeprazole and that H. pylori inferction induced a strong cyclooxygenase-2 expression resulting in excessive biosynthesis of gastroprotective prostaglandins, which should in turn counteract NSAID-induced gastropathy and heal existing ulcer. Other investigators claim that H. pylori infection acts synergistically with NSAID on ulcer development, therefore, H. pylori should be eradicated, particularly at the start of long-term NSAID therapy. Maastricht 2-2000 consensus also recommends eradication prior to NSAID treatment, but this eradication does not appear to accelerate ulcer healing or to prevent the recurrent ulcers in NSAID users. Our studies in almost 6000 dyspeptic patients undergoing upper endoscopy and [13C]-urea breath test (UBT) revealed that about 70 p.c. of these patients are H. pylori (+) and about 30.6 p.c. of these develop gastroduodenal ulcers. Of these ulcers, over 70 p.c. were H. pylori (+) positive, 12 p.c. NSAID (+), 8 p.c. both H. pylori (+) and NSAID (+), while 22 p.c. ulcers were H. pylori (-) and NSAID (-) or "idiopathic" ulcers...

691/2523

Tytuł oryginału: Interaction of the neurosteroid alphaxalone with conventional antiepileptic drugs in different types of experimental seizures.
Autorzy: Borowicz Kinga K., Zadrożniak Marek, Świąder Mariusz, Kowalska Aneta, Kleinrok Zdzisław, Czuczwar Stanisław J.
Źródło: Eur. J. Pharmacol. 2002: 449 (1/2) s.85-90, tab., bibliogr. [30] poz.
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: A number of neurosteroids exert antiseizure and/or neuroprotective properties. The aim of this study was to evaluate the effect of the neurosteroid alphalone on the protective action of conventional antiepileptics in four seizure test. Alphaxalone (up to 5 mg/kg) did not exert a signifcant action against amygdala-kindled seizures in rats, or against pentetrazole- or aminophylline-induced convulsion in mice. The neuroactive steroid at the dose of 2.5 mg/kg significantly raised the threshold for electroconvulsions in mice. at 2.5 mg/kg, alpaxalone diminished the protective activity of valproate against maximal electroshock and at 2.5 - 5 mg/kg against pentetrazole-induced seizures in mice. However, alphaxalone (2.5 mg/kg) did not affect the protective activity of carbamazepine, diphenylhydantoin, phenobarbital or clonazepam against maximal electroshock and 5 mg/kg did not affect that of phenobarbital, clonazepan and ethosuximide against pentetrazole-induced convulsions. Insignificant results were also obtained in the case of co-administration of alphaxalone with phenobarbital, valproate, clonazepam and carbamazepine against aminophylline-evoked seizures in mice. Also, in the kindling model of epilepsy, combinations of the neuroactive steroid (2.5 mg/kg) with valproate, carbamazepine, phenobarbital, diphenylhydantoin or clonazepam at their subprotective doses did not result in pro- or anticonvulsant activity. Valproate (284 mg/kg; the dose used in combination with alphaxalone) produced sgnificant memory deficits in mice. Alpxaxalone (2.5 mg/kg), valproate...

692/2523

Tytuł oryginału: Preconditioning with millimolar concentrations of vitamin C or N-acetylcysteine protects L6 muscle cells insulin-stimulated viability and DNA synthesis under oxidative stress.
Autorzy: Orzechowski Arkadiusz, Łokociejewska Małgorzata, Muras Patrycja, Hocquette Jean-Francois
Źródło: Life Sci. 2002: 71 (15) s.1793-1808, il., tab., bibliogr. 54 poz.
Sygnatura GBL: 304,948

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The effect of reactive oxygen/nitrogen species (ROS/RNS)(hydrogen peroxide - H2O2, superoxide anion radical O2ú- and hydroxyl radical úOH - the reaction products of hypoxanthine/xanthine oxidase system), nitric oxide (NOú from sodium nitroprusside - SNP), and peroxynitrite (ONOO- from 3-morpholinosydonimine - SIN-1) on insulin mitogenic effect was studied in L6 muscle cells after one day pretreatment with/or without antioxidants.ROS/RNS inhibited insulin-induced mitogenicity (DNA synthesis). Insulin (0.1 ćM), however, markedly improved mitogenecity in the muscle cells treated with treated with increased concentrations (0.1, 0.5, 1 mM) of donors of H2O2, Oú2-, úOH, ONOO- and NOú. Cell viability assessed by morphological criteria was also monitored. Massive apoptosis was induced by 1 mM of donors of H2O2 and ONOO-, while NOú additionaly induced necrotic cell death. Taken together, these results have shown that ROS/RNS provide a good explanation for the developing resistance to the growth promoting activity of insulin in myoblasts under conditions of oxidative or nitrosative stress. Cell viability showed that neither donor induced cell death when given below 0.5 mM.In order to confirm the deleterious effects of ROS/RNS prior to the subsequent treatment with ROS/RNS plus insulin one day pretreatment with selected antioxidants (sodium ascorbate - ASC (0.01, 01, 1 mM), or N-acetylcysteine - NAC (0.1, 1, 10 mM) was carried out. Surprisingly, at a low dose (micromolar) antioxidants did not abrogate and even worsened the concentration-dependent effects of ROS/RNS...

693/2523

Tytuł oryginału: Long-term results of surgery for early gastric cancer.
Autorzy: Popiela T., Kulig J., Kołodziejczyk P., Sierzega M.
Źródło: Br. J. Surg. 2002: 89 (8) s.1035-1042, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 310,131

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bacground: Gastrectomy for early gastric cancer, is widely accepted as an adequate therapeutic method. Recent developments of less invasive procedures require the identification who will benefit from such an approach. Methods: A retrospective study was undertaken of 238 patients with early gastric cancer who underwent gastrectomy from 1977 to 1999. Clinicopathological data relating to survival were evaluated. Results: Analysis of 33 node-positive patients (14 per cent) revealed a tumour diameter greater than 20 mm (P = 0.011), depressed macroscopic type (P 0.05), diffuse histological type (P 0.001), poor tumour differentiation ( P 0.001) and infiltration of the submucosal layer (P 0.002) as factors assopciated with lymph node metastasis. Multivariate analysis found diffuse histological type to be an independent risk factor. The overall 5-year survival rate was 87 p.c., and was significantly better in patients who underwent radical lymphadenectomy than in those who had regional lymph node dissection (92 versus 78 p.c.; P 0.01). Similarly, patients younger than 65 years had a more favourable 5-year survival rate (90 p.c.) than oder ones (77 p.c.). Multivariate analysis with the Cox proportional hazards model confirmed patient age and type of lymphadenectomy as independent prognostic factors. Conclusion: The findings suggest that extended lymph node dissection may be beneficial for some patients with early cancer, althoug randomized clinical trials are needed to evaluate this observation further.

694/2523

Tytuł oryginału: Usefulness of endothelin-1 concentration in capillary blood in patients with mitral stenosis as a predictor of regression of pulmonary hypertension after mitral valve replacement or valvuloplasty.
Autorzy: Snopek Grażyna, Pogorzelska Hanna, Rywik Tomasz M., Browarek Aldona, Janas Jadwiga, Korewicki Jerzy
Źródło: Am. J. Cardiol. 2002: 90 (2) s.188-189, il., tab., bibliogr. 11 poz.
Sygnatura GBL: 304,619

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

695/2523

Tytuł oryginału: Isolation and characterization of enterohaemorrhagic Escherichia coli O157:H7 from cattle in Belgium and Poland.
Autorzy: Tutenel A. V., Pierard D., Uradziński J., Jóźwik E., Pastuszczak M., Van Hende J., Uyttendaele M., Debevere J., Cheasty T., Van Hoof J., De Zutter L.
Źródło: Epidemiol. Infect. 2002: 129 (1) s.41-47, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 313,292

Hasła klasyfikacyjne GBL:

weterynaria

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

bydło

Streszczenie angielskie: EHEC 0157 were isolated from faeces of Belgian and Polish beef slaughter cattle. In Belgium, 1281 faecal samples were analysed by immunomagnetic separation [IMS] after enrichment in buffered peptone water from June 1998 till July 1999. Eighty-one samples (6.3 p.c.) were positive for E. coli O157. Phage type 8 was most frequently found. Bulls between 1 and 2 years old., slaughtered in September and October were most frequently found positive. Atypical biochemical features were observed in some isolates: 22 (27 p.c.) isolates were urease positive and 1 (1ú2 p.c.) isolate was unable to ferment lactose. In Poland, 551 faecal samples, taken from January 1999 till December 1999, were examined using exactly the same techniques. Four faecal samples (0ú7 p.c.) were positive for 0157 EHEC, yielding seven phage type 8 isolates. All positive samples were from cattle younger than 2 years. Positive samples occurred in August, September and October.

696/2523

Tytuł oryginału: Novijj oksidaznijj metod viznachenija vmistu L-laktatu.
Tytuł angielski: A new oxidase-coupled colorimetric method for L-lactate assay.
Autorzy: Pavlishko G. M., Majjdan M. M., Sibirnijj A. A., Gonchar M. V.
Źródło: Ukr. Biokhim. Zh. 2002: 74 (2) s.134-139, il., tab., bibliogr. 9 poz., sum.

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A new oxidase-coupled colorimetric method for analysis of L-lactate in biological fluids has been developed withous use of peroxidase. The methods is based on lactate oxidase-catalysed transformation lactate to pyruvate which is determined photometroically in the next dye-produing reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of ferric ions. Sensivity of the method is estimated as 0.1 micromole of analyte in 4-ml of reaction mixture. Linearity is observed in the range 0.1 - 1.0 micromole of L-lactate in sample (r = 0.99943; p 0.0001). The developed method has been adapted for assay of L=lactic acid in kefirs and yogurts.

697/2523

Tytuł oryginału: Improved reperfusion and clinical outcome with enoxaparin as an adjunct to streptokinase thrombolysis in acute myocardial infarction.
Autorzy: Simoons M. L., Krzemińska-Pakuła M., Alonso A., Goodman S. G., Kali A., Loos U., Gosset F., Louer V., Bigonzi F.
Źródło: Eur. Heart J. 2002: 23 (16) s.1282-1290, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 306,213

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Aims. To establish whether the addition of enoxaparin (a low-molecular-weight heparin0 to streptokinase therapy improves early and sustained patency and clinical outcome in patients with evolving myocardial infarction. Methods and results. A total of 496 patients with acute myocardial infarction treated with streptokinase were randomized to an intravenous bolus (30 mg) and subcutaneous injections (1 mgúkg-1 twice daily) of enoxaparin (n = 253), or placebo (n = 243) for 3-8 days. The median duration of treatment in both groups was 5 days. ST-segment resolution at 90 min and 180 min measured by electrocardiogram was improved in patients receiving enoxaparin. Complete, partial and no ST-segment resolution at 180 min was observed in 36 p.c., 44 p.c. and 19 p.c. in the enoxaparin group vs 25 p.c., 44 p.c. and 31 p.c. in the placebo group, rspectively (P = 0.004). Assessment of the primary end-point revealed improved TIMI-3 flow with enoxaparin vs placebo (70 p.c. vs 58 p.c., P = 0ú01). Combined TIMI-2 and -3 flow was also improved (88 p.c. vs 72 p.c., P = 0ú001), as was TIMI frame count (P = 0ú003). The triple clinical end-point of death, reinfarction and recurrent angina at 30 days was reduced with enoxaparin (13 p.c. vs 21 p.c., P = 0.03). Conclusion. Streptokinase in combination with enoxaparin is associated with better ST-segment resolution and better angiographic patency at days 5-10, suggesting more effective reperfusion. This was associated with a significant reduction in clinical events, indicating less reocclusion.

698/2523

Tytuł oryginału: Influence of agents affecting voltage-dependent calcium channels and dantrolene on the anticonvulsant action of the AMPA/kainate receptor antagonist LY 300164 in mice.
Autorzy: Świąder Mariusz, Borowicz Kinga K., Porębiak Jacek, Kleinrok Zdzisław, Czuczwar Stanisław J.
Źródło: Eur. Neuropsychopharmacol. 2002: 12 (4) s.311-319, il., tab., bibliogr. s. 317-319
Sygnatura GBL: 313,361

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: It was previously documented that calcium (Caý+) channel inhibitors intensified the protective effects of conventional antiepileptics against electroconvulsions in mice. The aim of this study was to evaluate the effects of Caý+ channel inhibitors (nifedipine, nicardipine and fluarizine) on the anticonvulsant action of the new AMPA/kainate receptor antagonist, 7-acetyl-3-94-aminophenyl)-8,9-dihydro-8-methyl-7H--1,3-dioxazolo[4,5-h][2,3]-benzodiazepine (LY 300164), against maximal electroshock (MES)-induced seizures in mice. Dantrolene (an inhibitor of Caý+ release from intracellular stroes) was also included. Nifedipine (30 mg/kg) and flunarizine (15 mg/kg) raised the threshold for electroconvulsions, being ineffective at lower dose. Nicardipine (up to 30 mg/kg) and dantrolene (up to 20 mg/kg) did not affect this parameter. Flunarizine (10 mg/kg), nicardipine (20 mg/kg) and dantrolene (20 mg/kg) potentiated the efficacy of LY 300164 against MES. However, nicardipine (at 20 mg/kg) raised the free plasma concentraion of LY 300164. Nifedipine (30 mg/kg), given even in a dose raising the electroconvulsive threshold, did not significantly alter the protective effect of LY 300164 against MES. Furthermore, the Caý+ channel agonist - BY k-8644 (at mg/kg) did not influence the protection offered by LY 300164 against MES. Finally, this Caý+ channel activator did not affect the enhanced efficacy of LY 300164 by Caý+ channel modulators. The only exception was the combination of LY 300164 with flunarizine. Combined treatment with LY 300164 and dantrolene (20 mg/kg)...

699/2523

Tytuł oryginału: Molsidomine potentiates the protective activity of GYKI 52466, a non-NMDA antagonist, MK-801, a non-competitive NMDA antagonist, and riluzole against electroconvulsions in mice.
Autorzy: Tutka Piotr, Olszewski Krzysztof, Woźniak Małgorzata, Kleinrok Zdzisław, Czuczwar Stanisław J., Wielosz Marian
Źródło: Eur. Neuropsychopharmacol. 2002: 12 (4) s.321-326, tab., bibliogr. [35] poz.
Sygnatura GBL: 313,361

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: The influence of molsidomine, a donor of nitric oxide (NO), L-arginine, a substrate for NO synthesis, and NG-nitro-L-arginine (NNA), an inhibitor of NO synthase, on the protective activity of CGP 40116, GYKI 52466, MK-801, and riluzole against electroconvulsions was studied in mice. Molsidomine (100 mg kg-1; i.p.) potentiated the protective activity of GYKI 52466, MK-801, and riluzole but did not influence the protection offered by CGP 40116. In contrast to molsidomine, L-arginine (500 mg kg-1, i.p.) did not impair the protective activity of any anticonvulsant. In a dose of 40 mg kg-1, NNA administered i.p. did not afect the protection offered by any excitatory amino acid antagonists and riluzole. Combinations of molsidomine with either GYKI 52466 or MK-801 as well as riluzole did not cause a memory deficit in the passive avoidance task. However, the combined treatment of molsidomine with these anticonvulsant resulted in a motor impairment quantified by the chimney test. The lack of effect of L-arginine and NNA on the protective activity of excitatory amino acid antagonist suggests that molsidomine-evoked alterations in the protection provided by some excitatory amino acid antagonists against electroconvulsions are independent of the NO pathway.

700/2523

Tytuł oryginału: Differential effects of repeated imipramine on hippocampal responsiveness to adenosine and serotonin.
Autorzy: Zahorodna Agnieszka, Bijak Maria, Hess Grzegorz
Źródło: Eur. Neuropsychopharmacol. 2002: 12 (4) s.355-360, il., bibliogr. [23] poz.
Sygnatura GBL: 313,361

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Imipramine-induced enhacement of the inhibitory action of 5-HT1A receptor activation in hippocampal pyramidal neurons has been attributed to alterations in the transduction mechanism that involves G protein-dependent opening of K+ channels. Postsynaptic 5-HT1A and adenosine A1 receptors may share that transduction pathway. We investigated the influence of repeated imipramine administration on 5-ht1A and adenosine A1 receptor-mediated effects in rat hippocampal slices. Repeated imipramine selectively enhanced the postsynaptic effects of 5-HT1A receptor activation hyperpolarization and reduction of input resistance of neurons and reduction of the population spike amplitude. In contrast, after imipramine treatment only the presynaptic effect of adenosine receptor agonist, a decrease of the field excitatory postsynaptic potential, was enhanced. The data demonstrate that alterations in the presumed common transduction mechanism that was postulated for the 5-HT1A and adenosine A1 receptor-mediated activation of K+ channels are not involved in the effect of repeated imipramine administration.

701/2523

Tytuł oryginału: Comparison of the Ames test and a newly developed assay for detection of mutagenic pollution of marine environments.
Autorzy: Czyż Agata, Szpilewska Hanna, Dutkiewicz Rafał, Kowalska Wioletta, Biniewska-Godlewska Anna, Węgrzyn Grzegorz
Źródło: Mutat. Res 2002: 519 (1/2) s.67-74, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 304,952

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A new assay for detection of mutagenic pollution of marine environments, based on the use of a series of genetically modified Vibrio harvbeyi strains, was developed recently. Here, we compared this assay with a commonly used Ames test, employing mutants of Salmonella enterica serovar Typhimurium. We found that survival of S. enterica serovar Typhimurium inartificial marine water and in different samples of marine water is dramatically reduced relative to that of V. harveyi strains. This indicates that V. harveyi strains are significantly more useful in testing samples of marine water. Moreover, sensitivity of both assays was compared. We found that using the V. harveyi assay it is possible to detect significantly (from a few to several times) lower concentrations of typical chemical mutagents than employing the Ames test. Although the higher sensitivity of the V. harveyi test relative to the Ames assay may be of minor importance when testing mutagenicity of certain chemicals in their pure from under laboratory conditions, this feature is very important in testing samples of marine water. Therefore, we assume that the V. harveyi assay may be an adequate test for detection of mutagenic pollution of marine environments.

702/2523

Tytuł oryginału: Prof. dr hab. Zygmunt Albert, 30.10.1908 - 07.10.2001.
Autorzy: Jeleń Michał
Źródło: Pol. J. Pathol. 2002: 53 (1) s.3-4
Sygnatura GBL: 301,852

Typ dokumentu:

praca biograficzna

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Albert

Zygmunt 1908-2001

703/2523

Tytuł oryginału: Prof. dr hab. Daniel Chibowski, 29.10.1932 - 04.08.2001.
Autorzy: Korobowicz Elżbieta
Źródło: Pol. J. Pathol. 2002: 53 (1) s.5-6
Sygnatura GBL: 301,852

Typ dokumentu:

praca biograficzna

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Chibowski

Daniel 1932-2001

704/2523

Tytuł oryginału: Bovine spongiform encephalopathy update.
Autorzy: Bradley Ray
Źródło: Pol. J. Pathol. 2002: 53 (1) s.7-16, il., tab., bibliogr. 24 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

weterynaria

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

koty

bydło

Streszczenie angielskie: Bovine spongiform encephalopathy (BSE) is a zoonosis being the origin of variant Creutzefeldt-Jacob disease and an important cattle disease in its own right. Countries have been show to learn the importance of protecting, not only their cattle populations, but also their human populations. Since 2000, several additional European countries have reported BSE in native-born stock and this has led to a concern about the BSE status of countries that have imported cattle and cattle products from infected countries. Extensive feed and offal bans and application of newly-developed, "Rapid" tests for prion protein in central nervous tissue of targed, high-risk animals and slaughter cattle over 30 months old now provides the tools whereby the public are fully protected and BSE can be eradicated.

705/2523

Tytuł oryginału: Prognostic significance of CD44v6 and nm23 protein immunoexpression in laryngeal squamous cell carcinoma.
Autorzy: Sikorska Beata, Danilewicz Marian, Wągrowska-Danilewicz Małgorzata
Źródło: Pol. J. Pathol. 2002: 53 (1) s.17-24, il., bibliogr. 34 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

badanie porównawcze

Wskaźnik treści:

ludzie

Streszczenie angielskie: In several tumours expression of CD44 and nm23 protein has been linked to tumour progression and metastasis. The aim of this study was to determine if there is any relationship between CD44v6 and nm23 protein immunoreactivity in squamous cancer cells and prognosis in patients with laryngeal carcinoma. The samples of human larynx cancer from patients with lymph node metastases and without lymph node involvement were analysed for CD44v6 and nm23 protein expression by immunohistochemistry using monoclonal antibodies. There was no significant difference in CD44v6 immunoexpression in cancer cells in patients with and without metastases. The statistical analysis did not reveal difference in survival time between patients with high and low CD44v6 immunoreactivity. The expression of nm23 protein in laryngeal cancer in patients with lymphe node metastases was significantly decreased in comparison with patients without metastases. Analysis of survival revealed that patients with lower expression of nm23 protein in tumour tissue had significantly shorter survival than patients with higher nm23 immunoreactivity. The results suggest that nm23 protein immunoreactivity can be useful in predicting tumour behviour in laryngeal cancer patients and indicate a lack of association between CD44v6 immunoexpression and tumour progression in those patients.

706/2523

Tytuł oryginału: Prognostic significance of selected immunohistochemical parameters in patients with invasive breast carcinoma concomitant with ductal carcinoma in situ.
Autorzy: Patla Anna, Rudnicka-Sosin Lucyna, Pawlęga Janusz, Stachura Jerzy
Źródło: Pol. J. Pathol. 2002: 53 (1) s.25-27, tab., bibliogr. 19 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The purpose of the present study was to carry out multivariate analysis of the effect of the expression of estrogen and progesterone receptors, p53, proliferative antigen (ki67) and c-erbB-2 on 5-year survival in patients with invasive breast carcinoma concomitant with duxtal carcinoma in situ. Material study consisted of tissue specimens obtained from 48 patients undergoing modified Patey's mastectomy between 1991 and 1998. Univariate analysis rervealed that the variables significantly affecting survival were tumour size on gross examination and the level of estrogen and progesterone receptors in the cells of invasive ductal carcinoma of the breast (for the level of significance p = 0.05). The Cox regression a significant effect on survival was the level of estrogen receptors in invasive cancer cells.

707/2523

Tytuł oryginału: Synchronous serrated adenoma of the appendix and high-grade ovarian carcinoma: a case demonstrating different origin of the two neoplasms.
Autorzy: Rudzki Zbigniew, Zazula Monika, Białas Magdalena, Klimek Marek, Stachura Jerzy
Źródło: Pol. J. Pathol. 2002: 53 (1) s.29-34, il., bibliogr. 12 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Association of mucinous adenomas of the appendix and mucinous ovarian tumors is well known. The origin of the ovarian tumor (metastases from the appendix vs independent primary) is still debated. Serrated adenoma is a rare neoplasm of the distal gastrointestinal tract, and its precancerous role in the colorectum was recently postulated. A 74-year-old patient was subjected to hysterectomy with routine appendectomy due to a 17-cm tumor of her right ovary. Histological examination revealed a high-grade ovarian adenocarcinoma with peritoneal involvement. The appendix, grossly unremarkable, harbored a serrated adenoma with no evidence of invasion or malignant transformation. Immunohistochemical examination revealed CD7+, CK20-phenotype of the ovarian and reverse (CK7-, CK20+) phenotype of the appendiceal tumor. Microsatelite analysis demonstrated microsatellite instability (MSI-high) within the serrated adenoma (4/5 markers with positive amplification) and no MSI (0/6 amplified markers) in the samples from the ovarian carcinoma, its metastases and the univolved uterine cervix. There were also differences in LOH pattern between the ovarian adenocarcinoma and the serrated adenoma. The findings suggest two independent primaries with profound differences in tumorigenetic pathways of both lesions. To the best of our knowledge this the first report of synchronous serrated adenoma of the appendix and ovarian carcinoma.

708/2523

Tytuł oryginału: Fatal post-transplant lymphoproliferative disorder following allogeneic bone marrow transplantation for aplastic anemia.
Autorzy: Rudzki Zbigniew, Werda Larissa, Piątkowska-Jakubas Beata, Mensah Patrycja, Zazula Monika, Białas Magdalena, Skotnicki Aleksander B., Stachura Jerzy
Źródło: Pol. J. Pathol. 2002: 53 (1) s.35-40, il., bibliogr. 13 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Post-transplant lymphoproliferative disorder (PTLD) constitutes serious complication of allogeneic bone marrow transplantation. We describe a case of PTLD in a twenty-six-old male treated with bone marrow transplantation for aplastic anemia of unknown case. The patient received unmanipulated marrow graft from his HLA-matched brother. Fifty-one days post transplant he developed progressive enlargement of cervical lymph nodes, followed by hepatosplenomegaly and generalized lymphadenopathy. Polymorphic PTLD was diagnosed basing on the lymph node histopathology, positive EBV detection, flow cytometry and IgH rearrangenement studies proving monoclonality (capillary electrophoresis with ABI PRISM 310 genetic Analyzer). There was no response to anti- CD20 antibody, cessation of immunosuppression, donor lymphocyte infusion and cystatic therapy. The patient died on the 65th day of multiple organ failure. We discuss the diagnostics and management of PTLD in the setting of bone marrow transplantation.

709/2523

Tytuł oryginału: Characteristics of Candida albicans strains isolated from the oral cavity in HIV-positive patients.
Tytuł polski: Charakterystyka szczepów Candida albicans wyizolowanych z jamy ustnej pacjentów HIV-dodatnich.
Autorzy: Macura Anna B., Bort Artur
Źródło: Mikol. Lek. 2002: 9 (1) s.17-25, il., tab., bibliogr. 14 poz., sum.
Sygnatura GBL: 313,181

Hasła klasyfikacyjne GBL:

aIDS

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Badaniem objęto 35 szczepów C. albicans wyizolowanych obficie z jamy ustnej pacjentów HIV-dodatnich oraz 15 szczepów C. albicans izolowanych od osób zdrowych HIV-ujemnych. Do oceny aktywności enzymatycznej użyto testu API ZYM firmy bioMrieux, ocenę wrażliwości na leki przeciwgrzybicze przeprowadzono przy użyciu testu TAB Fungus oraz metody dyfuzujno-krążkowej. Genotypowanie szczepów C. albicans wykonano techniką PCR - fingerprinting. W tym badaniu uwzględniono 24 szczepy C. albicans pochodzące z obfitego wzrostu od pacjentów HIV+ i 11 szczepów C. albicans z grupy porównawczej. (HIV-ujemnych). W badaniu nie stwierdzono istotnych różnic między szczepami pochodzącymi od pacjentów zarażonych HIV i zdrowych pod względem aktywności enzymatycznej i wrażliwości na antymiotyki. W obydwu grupach najczęściej występowały szczepy o biotypach L i M, przy czym w grupie pacjentów HIV+ istnała zależność między biotypem M i występowaniem objawów klinicznych grzybicy. Opisano nowy biotyp S u 4 szczepów. Analiza genotypów C. albicans wykazała zróżnicowanie genetyczne wśród szczepów wyizolowanych w obu grupach.

Streszczenie angielskie: The study was carried out on 35 C. albicans strains isolated from an abudant growth in the oral cavity of HIV+ patients and on 15 strains isolated from HIV negative healthy volunteers. Enzymatic activity was evaluated by using API ZYM test, manufactured by bioMrieux, drug susceptibility was tested by using both ATB Fungus test and disk-diffusion method. Genotyping of the C. albicans strains was performed by using PCR fingeprinting. Twenty four C. albicans strains were isolated from abundant growth in HIV+ patients while 11 C. albicans strains originated from the control group (HIV-). No significant differences were found between the strains originating from HIV+ patients and healthy persons as regards enzymatic activity and drug susceptibility. The L and M biotype strains were frequent in both of the groups. There was a relationship between M biotype and the presence of clinical manifestations of mycosis in the HIV+ group. A new biotype was detected in four strains; it was described as S biotype. The analysis of genotypes revealed a genetic variability of C. albicans genotypes isolated in both of the group.

710/2523

Tytuł oryginału: Cyproterone, norethindrone, medroxyprogesterone and levonorgestrel are less potent local human growth hormone and insulin-like growth factor I secretion stimulators than progesterone in human breast cancer explants expressing the estrogen receptor.
Autorzy: Milewicz T., Kołodziejczyk J., Krzysiek J., Basta A., Sztefko K., Kurek S., Stachura J., Gregoraszczuk E. L.
Źródło: Gynecol. Endocrinol. 2002: 16 (4) s.319-329, il., bibliogr. 57 poz.
Sygnatura GBL: 313,072

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The aim of the present study was to compare the ability of natural progesterone and synthetic progestins to stimulate local growth hormone (GH) and insulin-like growth factor I (IGF-I) secretion by breast cancer explants. Explants obtained during surgery were divided according to their estrogen/progesterone receptor phenotype - ER(+)PR(-); ER(+)PR(+); ER(-)PR(+) - as determined by immunocytochemistry. Natural progesterone (10**-5 mol/l) and synthetic progestins (cyproterone acetate (5 x 10**-7 mol/l), norethindrone (10**-5 mol/l), medroxyprogesterone acetate (10**-7 mol/l), and levonorgestrel (10**-7 mol/l) were tested in vitro for their ability to induce secretion of proliferation-promoting agents such as human GH (hGH) and IGF-I. All hormone-dependent breast cancer cell types responded to progesterone stimulation with increased local hGH secretion, while in the non-malignant tissue this effect was observed only in PR(+) cells. Moreover, progesterone in only PR(+) cells in vitro stimulated local IGF-I secretion by both malignant and non-malignant tissue. Medroxyprogesterone and levonorgestrel increased GH secretion by both malignant and non-malignant ER(-)PR(+) breast cancer explants, while cypropterone stimulated it only in non-malignant tissue. None of the synthetic progestins tested in this experiment exerted an effect on GH secretion by both malignant and non-malignant tissue of ER(+) breast cancer explants. The present data additionally showed that, apart from cyproterone, which increased IGF-1 secretion in the same manner as progesterone by both malignant and non-malignant ER(-)PR(+) breast explants...

711/2523

Tytuł oryginału: Turner's syndrome followed by acromegaly in the third decade of life: an unusual coincidence of two rare conditions.
Autorzy: Bolanowski B., Łomna-Bogdanov E., Kosmala W., Malczewska J., Ślęzak R., Zadrożna B., Podgórski J. K.
Źródło: Gynecol. Endocrinol. 2002: 16 (4) s.331-334, il., tab., bibliogr. 9 poz.
Sygnatura GBL: 313,072

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We present an unusual coincidence of acromegaly and Turner's syndrome. A girl was diagnosed with Turner's syndrome when she presented with short stature, primary amenorrhea, Hashimoto's thyroiditis, and some heart and renal anomalies. No therapy with growth hormone and only a few months treatment with estrogen-progestin was given. A typical picture of acromegaly occurred in the third decade of her life. Bone radiographs and densitometry suggested the more pronounced influence of acromegaly within the skeleton, but no features typical for acromegaly were found in the cardiovascular system. To our knowledge no case of coincidence of the above mentioned conditions has been reported to date. The influence of both of these conditions on bones and heart is discussed.

712/2523

Tytuł oryginału: Axial length measurements after cataract surgery in adults.
Autorzy: Lesiewska-Junk Hanna, Malukiewicz-Wiśniewska Grażyna
Źródło: Ophthalmologica 2002: 216 (4) s.239-241, tab., bibliogr. 8 poz.
Sygnatura GBL: 300,913

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: The purpose of the study was to check the difference in eye axial lenght before and after cataract surgery and to find out of possible correlation with refraction changes. The axial lenght was measured by ultrasound before and after cataract extraction with intraocular lens implantation. Keratomery measurements were also performed. The study comprised 32 eye. The mean difference in axial lenght was 0.53 mm. The mean difference in keratomery was 0.37 D. There was no correlation between these values. Conclusion: The change in axial length are not due to corneal curve changes. Perhaps in pseudophakic eyes different ultrasound velocity should be used.

713/2523

Tytuł oryginału: Are ultra-slow isoperiodic oscillations in rat intergeniculate leaflet neurons dependent on reciprocal connection with its contralaterally located counterpart?
Autorzy: Lewandowski Marian H., Błasiak Tomasz, Błasiak Anna
Źródło: Neurosci. Lett. 2002: 330 (3) s.243-246, il., bibliogr. 13 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: We investigated effects of the electrical lesion and/or chemical inactivation of intergeniculate leaflet (IGL) neurons on the ultra-slow isoperiodic neuronal oscillation of the contralaterally located IGL. The spontaneous extracellular activity of neurons, recorded simultaneously in both leaflets of the lateral geniculate nucleus, showed an ultradian oscillatory patern. In all our experiments, both the electrical lesion and the inactivation of neurons via the blockade of action potential generation did not cause any changes in the neuronal activity pattern in the contralaterally located geniculate leaflet. The obtained results show that a bilateral IGL connection is not necessary for the pattern of neuronal oscillation in the IGL. Hence the functional significance of a reciprocal connection between both lateral geniculate nucleus leaflets is still an open question.

714/2523

Tytuł oryginału: Ligands of the peroxisome proliferator-activated receptor-PPAR-ŕ reduce myocardial infarct size.
Autorzy: Wayman Nicole S., Ellis Bilinda L., Thiemermann Christoph
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR243-BR247, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: This study was desibned to investigate the effects of two, chemically distinct activators of PPAR-ŕ (clofibrate and WY14643) in a rat model of acute myocardial infaction. Material/Methods: Male Wistar rats were anesthetized with sodium thiopentone (120 mg/kg i.v.) and subjected to regional myocardial ischemia (for 25 min) and reperfusion (for 2 h). Area at risk was determined by injection of Evans Blue dye and infarct size after staining with nitroblue tetrazolium. Results: Pre-treatment of rats with the PPAR-ŕ agonists clofibrate (0.3 mg/kg) caused a significant reduction inmyocardial infarct size of 43 p.c. Similarly, pre-treatment of rats with the PPAR-ŕ agonists WY14643 (1 mg/kg) caused a significant reduction inmyocardial infarct size of 43 p.c. Conclusions: Taken together, these results show that ligands of PPAR-ŕ reduce the tissue necrosis associated with acute myocardial infarction. We propose that fibrates and other PPAR-ŕ agonists may be useful in conditions associated with ischemia-reperfusion of the heart and other organs.

715/2523

Tytuł oryginału: Biochemical characterization of the arginine degrading enzymes arginase and arginine deiminase and their effect on nitric oxide production.
Autorzy: Dillon Brian J., Holtsberg Frederick W., Ensor C. Mark, Bomalaski John S., Clark Mike A.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR248-BR253, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Nitric oxide (NO) is a biomediator believed to be synthesized primarily from extracellular arginine. Various methodologies have been used to inhibit NO synthesis so as to elucidate its physiological and pathophysiological functions. Several investigators have utilized various arginine degrading enzymes as a means of lowering extracellular arginine. Arginase, most commonly derived from mammalian sources, has been most often used. However, arginase has failed to inhibit NO synthesis. Herefore, a systematic biochemical characterization of arginase and arginine deiminase (ADI) derived from M. Hominus was undertaken. Material/Methods: The murine macrophage cell line N-9 was treated with either arginase or arginine deiminase to determine the effect on intracellular and extracellular arginine and nitric oxide production. Results: Arginase was found to have an alkaline pH optima (~9.5) with little enzyme activity at physiological pH. In contrast, the pH optima of ADI was ~6.5, retaining 70 p.c. of its activity at physiological pH. ADI had more than 1000 fold higher affinity for arginine (Km ~30 ćM for ADI vs ~45 mM for arginase), and was able to lower arginine levels to a much greater extent than arginase. ADI, unlike arginase, was effective in lowering extracelluar arginine in tissue culture media and inhibit NO production by the murine macrophage cell line N-9 in response to gamma interferon and LPS stimulation. Conclusions: These data suggest that ADI may be useful for delineating the role of NO in a variety of biological systems as well as determining the role of extracellular arginine in its synthesis.

716/2523

Tytuł oryginału: Betulinic acid, a potent inhibitor of eukaryotic topoisomerase I: identification of the inhibitory step, the major functional group responsible and development of more potent derivatives.
Autorzy: Chowdhury Arnab Roy, Mandal Suparna, Mittra Bidyottam, Sharma Shalini, Mukhopadhyay Sibabrata, Majumder Hemanta K.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR254-BR260, il., tab., bibliogr. 29 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Betulinic acid, a naturally abundant, plant derived, pentacyclic triterpenoid possesses anti-HIV, anti-malarial and anti-inflammatory properties and has recently emerged as a potent anti-tumor compound. This study explores the mode of action of betulinic acid on eukaryotic topoisomerase I and identifies the major functional group responsible along with more potent derivatives. Material/Methods: Topoisomerase I relaxation activity was electrophoretically measured by the decreased mobility of the relaxed monomers followed by ethidium bromide staining. DNA cleavage was studied by electrophoretic separation of the nicked monomers from the relaxed and supercoiled monomers in presence of ethidium bromide. In-vivo DNA cleavage was studied in blasted mouse splenocytes by the SDS-K+ trapping of 3H-DNA-topoisomerase I-camptothecin ternary complex. Results: Betulinic acid exerts its inhibitory effect by preventing topoisomerase I-DNA interaction as a result of which the 'cleavable complex' is not formed. In consequence, it also acts as an antagonist to camptothecin-mediated cleavage. A series of analogues modified at C-3, C-17 and C-20 positions of betulinic acid were subsequently assayed for inhibition of topoisomerase I catalytic activity. Replacemnt of the 17-carboxylic group reduces the inhibitory effect and decarboxylation leads to the complete loss of inhibitory effect. Conclusion: This study is the first detail report of betulinic acid as a very potent inhibitior of eukaryotic topoisomerase I and highlights the necessity of the carboxylic functional group. Dihydro betulinic acid is the most potent (IC50 = 0.5 ćM) pentacyclic triterpenoid to inhibit eukaryotic topoisomerase I till date and can be exploited as a strong candidate for anti-tumor drug designing.

717/2523

Tytuł oryginału: Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. P. 1: Spectrofluorometric analysis.
Autorzy: Parkhomenko Tatiana V., Klicenko Oksana A., Shavlovski Mikhail M., Kuznetsova Irina M., Uversky Vladimir N., Turoverov Konstantin K.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR261-BR265, il., bibliogr. 35 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Serum albumin (SA) is a major proteinacous component of the blood plasma. In the present study we report on the determination and structural characterization of the modified SA in the plasma of glomeruklonephritis (GN) patients. Fluorescence analysis of albumin-enriched fractions (AEF) isolated from serum of GN patients leads to the discovery of a proteinacous component with essential blue shift of intrinsic fluorescence. Material/Methods: AEF were isolated from serum blood of 35 GN patietns, 30 GN patietns with chronic renal failure (CRF) corrected by hemodialysis (HD) and 40 healthy donors. Their fluorescence chacteristics have been compared using variations in teh value of parameter A = I320/I365 that characterizes fluorescence spectrum position and shape. Results: The fluorescence spectra of AEF from GN and GN with CRF patient were blue-and red-shifted, respectively, in comparison with spectra of donors' serum. The value of parameter A was 1.27 ń 0.05 for protein preparateions from healthy donors, while this characteristic lies within range of 1.3-2.1 or 0.77 - 1.29 for Gn and Gn with CRF patients, resectively. Moreover, for GN patients, the magnitude of spectral parameter A was approaching control values during the treatment of disease. Conclusion: hSA enriched fractions isolated from GN patients or GN patients with CRF corrected by HD differ by their spectrofluorometric properties from those of healthy donors. This technique allows the detection of renal disease and the efficiency fo thier cure.

718/2523

Tytuł oryginału: Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. P. 2: Evidence for the enhancement of the haptoglobin. Level at the patholgoical conditions.
Autorzy: Parkhomenko Tatiana V., Klicenko Oxana A., Shavlovski Mikhail M., Poletaev Andrey I., Kuznetsova Irina M., Uversky Vladimir N., Turoverov Konstantin K.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR266-BR271, il., bibliogr. 59 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Human serum albumin (hSA), the major component in blood plasma, fulfills a fundamental biological role as a universal carrier and resevoir in blood plasma, tissues, and secretions. Becaues of essential diversity in the bound ligands, hSA was shown to possess significant structural divergence. In the first paper of this series we reported on that albumin-enriched fractions isolated from serum of glomerulonephritis (GN) patients possesses essential blue shift of intrinsic fluorescence. Identification of this component was the major goal of this study. Material/Methods: Partially purifies SA preparations were isolated from blood plasma of 19 GN patients and 8 healthy donors. These preparations have been analyzed by combination of chromatographic (gel-filtration and hydrophobic sorption) and spectrofluorometric techniques. Results: At least six differented albumin enriched fractions were isolated from blood of GN pateitns. High molecular weight protein fraction with blue shifted intrinsic fluorescence spectrum was segregated. Higher concentration of this fraction may be responsible for higher parameter A values, which is characteristic of protein preparations from GN patietns. Amino acid sequence showed that á-haptoglobin is one of the components of this fraction. Conclusion: Combination of chromatographic methods with monitoring intrinsic fluorescence spectra of albumin-enriched fractions from blood plasma of healthy donors and GN patients represents a useful tool for the description of hSA heterogeneity and for the detection of the changes in the level of disease-related compounds. This approach is also appropriate for the isolation of individual components, different in their parameter A values, from the blood plasma.

719/2523

Tytuł oryginału: Effect of hydroxyethyl starch (HAES) on degree and kinetics of erythrocyte aggregation studied with dielectric spectroscopy method.
Autorzy: Jaroszyński Witold, Keslinka Elżbieta, Wujtewicz Maria, Suchorzewska Janina, Kwiatkowski Bartłomiej
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR272-BR278, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Colloidal fluids, e.g. hydroxyethyl strach (HAES), used as blood plasma substituted during acute controlled normovolemic hemodilution may cause erythrocyte aggregation. The aim of the presented study was to assess the degree and rate of erythrocyte aggregation in the media containing different concentrations of HAES. Material/Methods: The experiments were carried out on hpearinized venous blood and on erythrocyte suspension in 0.9 p.c. NaCl. Results: Uncer stationary conditions, an increase of blood and erythrocyte suspension relative electric permitivity (î), with an increase of HAES concentration in the sample, was observed after HAES supplementation. Sample flow through a measuring chamber resulted in decrease of the îL value (determined for 100 kHz). Stopping the flow led to non-linear increase of îL values in the function of time, where two components - characterized by rapid and slow variability, respectively, could be distinguished. The increase was characterized by ç1 and ç2 relaxation times, which can be related respectively to the process of erythrocyte rouleaux formation and to the formation of 3-D network aggregates. Conclusions: 1) The values of çL are differentiated by the degree of erythrocyte aggregation due to the presence of HAES, both in whole blood and in erythrocyte suspension in 0.9 p.c. NaCl solution, 2) The degree of erythrocyte disaggregation due to flow increases with the increase of flow intensity and correlates with decreasing çL value, 3) ç1 and ç2, which characterize the kinetics of erythrocyte aggregation, indicate a difference between aggregation processes taking place in whole blood and after hemodilution with HAES.

720/2523

Tytuł oryginału: Chromosome alterations reflect clonal evolution in squamous cell carcinoma of the larynx.
Autorzy: Kujawski Maciej, Rydzanicz Małgorzata, Sarlomo-Rikala Maarit, Gabriel Andrzej, Szyfter Krzysztof
Źródło: Med. Sci. Monitor 2002: 8 (7) s.BR279-BR282, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: The mechanism of multistage carcinogenesis initiated by environmental carcinogens produces clonal evolution of initiated cells to the stage of metastasis. However, the simultaneous co-existence of various cell clones is not excluded. The analysis of chromosome alterations appears to be the best method to assess the clonal composition of a tumor. Material/Methods: Laryngeal tumor specimens and their corresponding metastases to the adjacent lymph nodes (20 pairs) were analysed by comparative genome hybridization (CGH). Results: The profile of gains and losses of DNA copy number was found to be fairly similar in the primary tumor location and in its metastasis. in this study the most frequent losses were found on 3p, 5q, 9p, 13p, and 13q, while gains occurred in 1q, 3q, 5p and 11q. The gains and losses were found more frequently in metastasis than in primary tumor, except for the loss of 3p and the gain of 5p. The bioogical function of the latter chromosome alterations seems to be limited to tumor growth, rather than metastasis formation. Conclusions: A comparison of the profiles of chromosomes alterations in primary tumor locations and metastases indicate that the progression of laryngeal cancer is connected with the clonal evolution of tumor cells.

721/2523

Tytuł oryginału: Plasma soluble L-selectin following cardiopulmonary bypass (CPB) in children: is it a marker of the postoperative course?
Autorzy: Dagan Ovdi, Prince Tal, Ben-Abraham Ron, Vidne Bernardo, Mishali David, Katz Yitshak, Keller Natan, Barzilay Zohar, Paret Gideon
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR467-CR472, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: There is increasing evidence that cytokine-inducible leukocyte-endothelial adhesion molecules are instrumental in the postoperative inflammatory response following cardiopulmonary bypass (CPB). L-selectin was shown to be one of those neutrophil-endothelial cell adhesion molecules. This study aimed to ivnestigate the relationship of teh soluble adhesion molecule, sL-selectin, and the postoperative course in children undergoing CPB. Material/Methods: To determine the time course of sL-selectin after CPB, serial blood samples of 9 children undergoing CPB were collected from the arterial line or from the bypass circuits preoperatively, on initiation of CPB and 1, 6, 12, 18, 24, and 48 hours postoperatively. Plasma was recovered immediately, aliqoted and frozedn at -70řC until use. Circulating sL-selectin molecules were measured witha sandwich enzyme-linked immunoabosrbent assay (ELISA) technique. there were significant changes in plasma levels of sL-selectin in patients following CPB, and these levels were associated with patient characteristics, operative variables and postoperative course. Low values of sL-selectin significantly correlated with inotropic support, low PRISM score, postoperative hypotension and fever. There was a significant association between the development of postoperative sepsis and low sL-selectin levels. No correlation was found between sL-selectin values and lactate concentration or neutrophil fount. Conclusions: Our results suggest a relation between CPB-induced mediators and both early and late clinical effects...

722/2523

Tytuł oryginału: Effect of renin-angiotensin system activation by dietary sodium restriction and upright position on plasma leptin concentration in patietns with essential hypertension.
Autorzy: Adamczak Marcin, Kokot Franciszek, Chudek Jerzy, Więcek Andrzej
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR473-CR477, tab., bibliogr. 43 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Both leptin and the renin-angiotensin system (RAS) are involved in the regulation of arterial blood pressure. This study was undertaken to assess the relationship between RAS and plasma leptin concentration in hypertensive patients under conditions of normal and restricted sodium supply and upright postion. Material/Methods: In 31 patietns with essential hypertension (EHP - 14 F, 17 M, age 44 ń 14 years, BMI 29.3 ń 6.4 kg/mý) and 8 healthy subjects (NHS - 4 F, 4 M, age 37 (17 years, BMI 25.3 ń 6.6 kg/mý) plasma leptin cocnentration, plasma renin activity (PRA), and 24-hour urinary sodium exretion (UNa) were evaluated twice: first on a diet containing 100-120 mmol sodium per day and after 8 hours overnight bed rest, and a seond time after 3 days of dietary sodium restriction (10-20 mmol daily) adn 3 horus in upright position. Results: Dietary sodium restriction and upright position was followed by a significant increase in PRA and decrease of UNa. By contrast, plasma leptin concentration showed a moderate decrease both in EHP and NHS. No significant correlation was found between PRA and plasma leptin concentrations in either of the groups examined. Cocnlusion: From the results obtained in this study we may conclude that dietary sodium restriction and upright positon exerts only a moderate effect on plasma leptin concentration, in contrast to PRA, in both hypertensive and normotensive subjects.

723/2523

Tytuł oryginału: Phenotypic characterization of infilatrates in dilated cardiomyopathy - diagnostic significance of T-lymphocytes and macrophages in inflammatory cardiomyopathy.
Autorzy: Noutsias Michel, Pauschinger Matthias, Schultheiss Heinz-Peter, Khl Uwe
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR478-CR487, il., tab., bibliogr. 55 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Dilated cardiomyopathy (DCM) is linked to inflammatory cardiomyopathy (InfCM). To gain a profound insight into the underlying mechanisms, we phenotypically characterized pan leukocytes (CD18), naive T-lymphocytes (CD3, CD2, CD4, CD8), activated lympocytes (LFA-1, LFA-3, VLA-4, ICAM-1, CD69, CD45RO), macrophages (Mac-1, 27E10), B-lymphocytes (CD19) and NK-cells (CD57) in DCM and control hearts. Material/Methods: Biopsies from DCM patietns (n = 164, LVEF 40 p.c.) and specimens from non-cardiac death causes (controls; n = 17) were immunostained. Biopsies exceeding 2.0 CD3+ lymphocytes per high power field/hpf and/or 1.5 CD3+ lymphocytes/hpf with numerous foci and HLA class I/DR abundance were evaluated positive for InfCM. Results: InfCM+ biopsies (n = 102; 63 p.c.) demonstrated significantly increased infiltrates with respect to all studied phenotypes except for CD19 and CD57 when compared with both DCM biopsies negative for InfCM (n = 62) and the controls, whereas the latter two groups did not differ (Tukey-Kramer analysis). Virtually all phenotypes correlated with one another in multivariate analysis (except for B-lymphocytes and NK cells). Whereas HLA class I/DR abundance was present in 14 p.c. of the controls and 26 p.c. of the DCM biopsies not yielding InflCM, InfCM+ biopsies demonstrated significantly ( 0.001) higher frequencies of HLA abundance (76 p.c.). Conclusions: The inflammatory process in InfCM comprises T-lymphocytes and macrophages, whereas B-lymphocytes and NK-cells are not significantly increased. InfCM is associated with HLA induction...

724/2523

Tytuł oryginału: Assessment of the prevalence of iron deficiency anemia, by serum ferritin, in pregnant women of Southern Iran.
Autorzy: Karimi Mehran, Kadivar Raheem, Yarmohammadi Hooman
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR488-CR492, tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Background: Iron-deficiency anemia (IDA) is a public health problem in the developing and even industrialized countries. Pregnant women and children under 5 years of age are among the high-risk population. Our main objectives in this study were to obtain the prevalence of IDA in pregnant women by routine methods and by serum ferritin. Material/Methods: We analysed the blood of 270 healthy pregnant mothers, 16 weeks of gestational age. A series of determinations were conducted to determine haemoglobin concentration (Hb); red blood cells count (RBC); serum ferritina and other indexes. Then a questionnaire for epidemiolgoical data, type of diet, level of education, laboratory data, etc. was filled. Results: The mean values (SD) of haematological indexes were as follows: Hb 12.07 ń 1.5 g/dl; serum ferritin 24.87 ń 19.32 ng/ml; mean corpuscular haemoglobin concentration (MCHC) 31.9 ń 1.4 g/dl; mean corpuscular volume (MCV) 82.2 ń 9 fl and mean corpuscular haemoglobin (MCH) 26.4 ń 3.2 pg. 28.5 p.c. of the subjects were anaemic at the time of the study according to serum ferritin (SF 12 ng/ml) and 16.7 p.c. fo the mothers had low serum Haemoglobin (Hb 11 g/dl) (P - 0.005). There was a positive correlation (r = 0.76; P = 0.01) between Hb concentration and serum ferritin levels. Conclusion: The prevalence of IDA was 28.5 p.c., which is the same as teh prevalence found in other developing coutnries (25-35 p.c.). This shows that in southern Iran we are still far behind the health status in the industrialized countries (5-8 p.c.).

725/2523

Tytuł oryginału: CT-guided transthoracic fine needle aspiration of pulmonary lesions: accuracy and complications in 294 patients.
Autorzy: Arslan Sulhattin, Yilmaz Adnan, Bayramgrler Birol, Uzman zlem, nver Edhem, Akkaya Esen
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR493-CR497, tab., bibliogr. 32 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of this study was to prospectively evaluate the effectiveness of CT-guided transthoracic fine needle aspiration in the diagnosis of pulmonary lesions and to determine the complication rate of this procedure. Material/Methods: A prospective review was udnertaken of 316 patietns who underwent CT-guided transthoracic fine needle aspiration performed at our center between October 2000 and April 2001. Twenty-two patietns were excluded because no final diagnosis was achieved. The present study included 294 patietns. All fine needle aspirations were performed with a 22-gauge aspirating needle under CT guidance. Results: An accurate diagnosis was made in 228 of 259 malignant lung lesions (88 p.c.). A speicific diagnosis was obtained in 34.3 p.c. of the benign lesions. The sensitivity for malignancy and specificity for benign lesions were 88 p.c. and 100 p.c., respectively. Positive and negative predictive values were 100 p.c. and 53 p.c., respectively. Tehre was no fals-positive diagnosis for malignancy. Sensitivity was 87 p.c. for centrally located lesions and 89.3 p.c. for peripherally located lesions (p 0.05). Complications included pneumothorax, 24 of 294 cases (8.2 p.c.); chest tube, 3 cases (12.5 p.c.); minor hemoptysis, 5 cases (1.7 p.c.); and pulmonary hemorrhage, 4 cases (1.4 p.c.). Conclusions: Our results suggest that this procedure has high diagnostic accuracy and an acceptable rate of complications.

726/2523

Tytuł oryginału: Spectrum and proteinase production of yeasts causing vaginitis in Saudi Arabian women.
Autorzy: Al-Hedaithy Saleh S. A.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR498-CR501, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Yeast vaginitis is a common infection. Data on the identity and prevalence of the etiological species would serve both epidemiological and therapeutic ends. Proteinase secreation by the invading yeast has been implicated in facilitating infection. Hence a prospective study was conducted to determine the species causing vaginitis in women from Saudi Arabia and the yeast's ability to produce proteinase. Material/Methods: The subjects were patients with clincial signs of vaginitis. A high vaginal swab specimen from each patient was cultured, and only women yielding heavy yeast growth were enrolled. The isolated yeasts were identified by routine procedures, then evaluated for proteinase production in a medium containing bovine serium albumin. Results: A total of 500 patients were studied. Candida albicans was the major species to cause vaginitis (59 p.c.), followed by C. glabrata (31 p.c.), C. tropicalis (4 p.c.), C. krusei (3.2 p.c.), Saccharomyces cerevisiae (1.6 p.c.), C. parapsilosis (0.6 p.c.), and C. kefyr (0.6 p.c.). All isolated of C. albicans and C. parapsilosis and 95 p.c. of C. ropicalis produced proteinase, while isolates from the remaining speices did not. Conclusions: These results indicate that 59.6 p.c. of yeast vaginitis in women from Saudi Arabia is caused by C. albicans, and 31.6 p.c. by C. glabrata. Of relatively low prevalence were C. tropicalis and C. krusei. The study also revealed that both proteinase producers and non-producers were involved in causing vaginitis, indicating that proteinase secretion is not an essential factor in the pathogeneis of yeast vaginitis.

727/2523

Tytuł oryginału: Changes of arterial pressure in patients with hyperthyroidism during therapy.
Autorzy: Marcisz Czesław, Jonderko Gerard, Kucharz Eugeniusz J.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR502-CR507, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Hyperthyroidism affects the circulatory system, producing an increase in cardiac output and an enhanced vascular bed volume. These phenomena are important in the regulation of blood pressure. The present study was designed to evaluate the effects of short- and longterm treatment of hyperthyroidism on arterial pressure, taking into account the indices of cardiac function and peripheral vessel resistance. Material/Methods: Fifty-one patients with hyperthyroidism and 30 healthy controls were investigated. The patients were examined before treatment, two weeks after initiation of therpay, and after attainment of a euthyroid state. Thiamazole was used as the antithyroid agent. Blood pressure and serum thyroid hormones were determined and ultrasonographic examination was performed on all teh subjects, after the application of a diet containing 120 mmol of sodium and 70 mmol of potassium for three days. Results: Patietns with hyperthyroidism had higher systolic blood pressure and lower diastolic blood pressure than the healthy controls. After short-term treatment, systolic blood pressure returned to normal, while diastolic blood pressure was normalized only after long-term treatment. Conclusion: Regulation of blood pressure in patients with hyperthyroidism is a complex process. Systolic blood pressure is mainly related to caridac output and returns to normal range shortly after the initiation of tehrapy, while diastolic blood pressure is related to peripheral vascualr resistance and is normalized after long-term treatment.

728/2523

Tytuł oryginału: Left atrial function in patients with renal transplantation.
Autorzy: Rysz Jacek, Goch Aleksander, Wilk Radosław, Grycewicz Tomasz, Luciak Marek, Goch Jan Henryk
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR508-CR511, tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Chronic renal failure leads to structural changes and cardiac functional abnormalities known as uremic cardiomyopathy. Eliminating excess water and improving the composition of the inner environment leads to at least partial cardiac function improvement, which is reflected primarily in favorable changes in left ventricular indices. The aim of our study was to evaluate left atrial function in patients after renal transplantation and compare them with clinically healthy subjects. Material/Methods: 10 renal transplant patients (2 women, 8 men; mean age 47.8 ń 6.4 years), treated before transplantation with repeated hemodialysis, were subjected to standard transthoracic echocardiographic examination. Atrial function was evaluated in M-mode and 2D projections and by cross-sectional Doppler echocardiography. The results obtained were compared with 16 healthy controls (9 women, 7 men; mean age 39.7 ń 9.4 years). Results: Maximal left atrial dimension (LAmax), left atrial dimension obtained in M-mode of the long axis in parasternal projection (LAa), ejection time (ETlp) and pre-ejection period (PEPlp) were significantly higher in renal transplant patietns than in healthy subjects. In both investigated groups there were no differences in minimal left atrial dimensions (LAmin), PEPlp/Etlp ratio, P wave time (P), left atrial fiber shortening fraction (FS p.c.lp), passive evacuate fraction (FBOlp), total left atrial fraction (FClp), or IElp ratio. Conclusion: Abnormal function of the left atrium in the course of uremia treated with repeated hemodialysis is not fully corrected after renal transplantation despite the elimination of many cardiovascular complciations observed in chronic renal disease.

729/2523

Tytuł oryginału: Sulfonylurea receptor gene 16-3 polymorphism - association with sulfonylurea or insulin treatment in type 2 diabetic subjects.
Autorzy: Zychma Marcin J., Gumprecht Janusz, Strojek Krzysztof, Grzeszczak Władysław, Moczulski Dariusz, Trautsolt Wanda, Karasek Dariusz
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR512-CR515, tab., bibliogr. 15 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

badanie porównawcze

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: The presence of a complex phenotype of type 2 diabetes results from impaired insulin secretion and action, whereas the emchanism of action of sulfonylurea derivatives, most commonly used int he treatment of type 2 diabetes, is based on their ability to directly inhibit the ATP-sensitive potassium channel (KATP), which leads to á-cell depolarization, subsequent influx of calcium and then insulin exocytosis. It has recently been demonstrated in healthy subjects that molecular variants of the gene encoding for the KATP subunit - sulfonylurea receptor gene (SUR1) are associated with a decreased response of insulin secretion to intravenous injection of tolbutamide, a suflonylurea derivative. In thid study we tested whether a molecular variant of the SUR1 gene, 16-3t, has a different distribution in type 2 diabetic patients with early failure of sulfonylurea therapy, compared to patients treatable iwth sufonylurea despite long diabetes duration. Material/Methods: The SUR1 polymorphism was genotyped in 68 type 2 diabetic patietns who required insulin treatment and had known diabetes duration ó 5 years, compared to 99 patients receiving oral agents (sulfonylurea alone or in combination with metformin or acarbose) with known diabetes duration of at least 15 years. Results: We obseverd no significant differences in SUR1 16-3t genotype distributions or allele frequencies between the two examined groups. Conclusion: Our study provides evidence against a major impact of the SUR1 c16-3t polymorphism on the long-term effectiveness of therapy with sulfonylurea derivatives in type 2 diabetic patients.

730/2523

Tytuł oryginału: Hepatitis B virus serologic markers and anti-hepatitis B vaccination in patietns with diabetes.
Autorzy: Halota Waldemar, Muszyńska Mirosława, Pawłowska Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR516-CR519, tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: In Poland HBV infections are mainly the result of nosocomial infections or risky behavior. Active prevention plays a crucial role in limiting such infections. Pateitns suffering from insulin-dependent diabetes (type 1) incur high risk of infection with hepatotropic viruses because of frequent hospitalization and blood tests. The aim of our study was to determine the frequency of cocurrence of serological markers of HBV infection among patietns with diabetes type 1, to evaluate the humoral response to HBV vaccine depending on dosage, and to evaluate the influence of past HBV infections on vaccination response. Material/Methods: 299 diabetic patients were vaccinated against hepatitis B with Engerix B in two doses (20 ćg or 40 ćg), on a 0-1-6 month schedule. The humoral response to the vaccination was evaluated in the 5th and 8th months after the first dose of vaccine according to sex, dose and previous detection of anti-HBc in serum. Serologic markers of HBV infection were detected with the immunoenzymatic-fluorescent ELFA method, with bioMerieux tests using the VIDAS system. Results: 98.7 p.c. of patients achieved protective anti-HBs titer after vaccination. Women respodned better than men. There were nod ifferences in mean anti-HBs concentration by vaccine dose. Patietns with previous serum anti-HBc achieved anti-HBs titers (p 0.00001). Concluion: The frequency of anti-HBc among patietns with diabetes confirms the widespread risk of HBV infection in teh Polish population. Diabetics should be vaccinated against hepatits B with standard doses. Diabetics with anti-HBc in serum can be vaccinated with a single dose.

731/2523

Tytuł oryginału: Ozone therapy and the activity of selected lysosomal enzymes in blood serum of patietns with lower limb ischaemia associated with obliterative atheromatosis.
Autorzy: Tafil-Klawe Małgorzata, Woźniak Alina, Drewa Tomasz, Ponikowska Irena, Drewa Joanna, Drewa Gerard, Włodarczyk Konrad, Olszewska Dorota, Klawe Jacek, Kozłowska Róża
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR520-CR525, tab., bibliogr. 49 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The paper compares the effects of ozone therpay and conventional balneological methods on health conidition of patietns with obliterative atheromatosis and on serum activity of three lysosomal enzymes. Material/Methods: Sixty-four patietns with lower limb ischaemia in the course of obliterative atheromatosis (without diabetes) were nerolled in the study. Thirty-two patietns were treated with ozone administered by intravenous infusions and 30-minute aerosol oxygen-ozone baths. A comparative group was formed of 32 patietns treated with traditional balneology. There was also a control group made u p of 30 healthy subjects. Ozone tehrapy as well as traditional balenology were administered daily for the period of 10 days, excluding Saturdays and Sundays. Blood for biochemical analysis was collected from elbow vein in the following time intervals: 24 hours before ozone therpy or classical balneology, one hour after therpay and on the 10th day of treatment. The activity of catehpsin D, acid phosphatase and arylsulphatase as well as the levels of ŕ-1-antitrypsin (protease inhibitor) were determined in blood serum of patietns with obliteraitve atheromatosis. Results: In patients who received ozone therapy the activity of analysed lysosomal hydrolases returned to the values typical for healthy subjects. Patietns' general condition also improved. The use of traditional balneological methods did not result in any significant change either in the activity of lysosomal hydrolases, the level of ŕ-1-antitrypsin or general condition of patients. Conclusions: Ozone therapy administered by intravenous infusions and aerosol oxygen-ozone baths of lower extremities yields much better therapeutic results in comparison with classical balneology.

732/2523

Tytuł oryginału: TH1/TH2 balance in the subretinal fluid of patients with rhegmatogenous retinal detachment.
Autorzy: Lewandowska-Furmanik Maria, Pożarowska Dorota, Pożarowski Piotr, Matysik Anna
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR526-CR528, tab., bibliogr. 6 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

okulistyka

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The pathogenesis of rhegmatogenous retinal detachemnts remains unclear, but some data suggest immune system involvement. Cytokines are substances secreted by cells in order to modulate the function of other cells. They exert primarily local effects. Because the TH1/TH2 balance is crucial in the immune response, the predominance of one cytokine profile in subretinal fluid may be of particular importance in understanding its pathogenesis. Material/Methods: We measurred the titres of IL-10 and tIL-12 in the subretinal fluid of 36 rhegmatogenous retinal detachment patients using ELISA assays. Results: The mean level of IL-10 and tIL-12 in the subretinal fluid was 4.75 pg/ml and 106.5 pg/ml, respectively. Conclusion: It would appear that IL-10 and IL-12 are produced by cells in subretinal fluid, and their presence may be evidence for an existing inflammatory process. Despite the tendency to decreased cytokine concnetration over time and the tendency to higher concentrations of IL-TH1- or TH2-type response was observed.

733/2523

Tytuł oryginału: Superoxide dismutase mimetic with catalase activity, EUK-134, attenuates the multiple organ injury and dysfunction caused by endotoxin in the rat.
Autorzy: Villa Bianca Roberta d'Emanuele di, Wayman Nicol S., McDonald Michelle C., Pinto Aldo, Sharpe Martyn A., Chatterjee Prabal K., Thiemermann Christoph
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR1-BR7, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Reactive oxygen species contribute to the multiple organ failure shock. Here we investigate the effects of a salen-manganese complex, which exhibits both superoxide dismutase and catalase activity (EUK-134), on the circulatory failure and the renal and liver injury and dysfunction caused by endotoxin in the anaesthetised (thiopentone, 120 mg/kg) rat. Male Wistar rats were anaesthetised with hiopentone sodium (120 kg i.p.) and instrumented for the measurements of systemic haemodynamics. Animals received lipopolysaccharide (LPS, E. coli, 6 mg/kg i.v.) or saline and were treated with either EUK-134 (0.3 or 1 mg/kg bolus injection folloowed by an infusion of 0.3 or 1 mg/kg/h) or its vehicle (saline). After 6 h of endotoxaemia, blood was taken to evaluate biochemical parameters of organ injury and dysfunction. All data are mean ń s.e. mean of n observations. Statisstical comparisons were made with a ANOVA followed by Dunner's test for mulitiple comparisons. Endotoxaemia for 6 h caused hypotension, renal dysfunction, liver injury, skeletal-muscle injury and pancreatic injury. Treatment of rats with EUK-134 attenuated the reanl dysfynction as well as the liver and skeletal muscle injury (but not the pancreatic injury) caused by endotoxin. Thus, an enhanced formation of reactive oxygen species importantly contribute to the organ injury and dysfunction associated wtih endotoxic shock. We propose that small molecules, which have the catalytic activity of both superoxide dismutase and catalase, may represent a novel therapeutic approach for the therapy of endotoxic shock.

734/2523

Tytuł oryginału: NOC/oFQ activates PKC and generates superoxide to impair hypotensive cerebrovasodilation after hypoxia/ischemia.
Autorzy: Armstead William M.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR8-BR14, il., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Previous studies have observed that hypotensive pial artery dilation was blunted following global cerebral ischemia in the piglet. In unrelated studies, superoxide (O-2) contributed to impaired hypotensive cerebrovasodilation following traumatic brain injury in the rat while the opioid nociceptin/orphanin FQ (NOC/oFQ) generated O-2 via activation of protein kianse C in the piglet. This study determined the contribution of NOC/oFQ, PKC activation and O-2 generation in hypoxic ischemic hypotensive cerebrovasodilation impairment. Anesthetized newborn pigs equipped with a closed cranial window were used. Global cerebral ischemia was produced via elevated intracranial pressure. Hypoxia, via inhalation of nitrogen, decreased P02 to 34 ń mmHg. Topical NOC/oFQ(10**-10 M), the CSF concentration following hypoxia/ischemia, had to effecton pial artery diameter by itself but attenuated hypotension (mean arterial blood pressure decrease of 44 ń 2 p.c.) induced pial artery dilation (33 ń 1 vs 19ń 2 p.c.). Coadministration of the PKC inhibiator chelerythrine (10**-7 M) or the O-2 scavenger polyethylene glycol superoxide dismutase and catalase (SODCAT) with NOC/oFQ (10**-10 M) partially prevented hypotensive pial dilation impariment (34 ń 2 vs 28 ń 1 p.c. fopr SODCAT). Hypotensive pial artery dilation was blunted by hypoxia/ischemia but such dilation was partially protected by the NOC/oFQ receptor antagonist [F/G] NOC/oFQ (1-13) NH2 (10**-6 M), chelerythine or SODCAT (34 ń 1 vs 7 + 2 vs 21 ń 2 p.c. for sham, H/I and H/I + SODCAT, respectively).

735/2523

Tytuł oryginału: Detection of gsp somatic mutation through direct sequencing of heteroduplex alleles disclosed by denaturing gradient gel electrophoresis.
Autorzy: Fragoso Maria C. Barisson Villares, Lado Valeria S., Latronico Ana C., Frazzatto Eliana T. Salgado, Russell Alan J., Mendonca Berenice B.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR15-BR18, il., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The identification of somatic mutations in tissues is often difficult when the number of normal alleles in the tissue far exceeds the number of mutation was not possible by direct sequencing and present a new approach that improves the identification of gsp somatic mutations. Genomic DNA was extracted from frozen tissue of a human ovarian stromal Leydig cell tumor. Exons 8 and 9 of the Gsŕ gene were amplified by PCR and despite the abnormal migration pattern at this first DGGE, direct sequencing of the PCR product did not reveal mutations, probably due to the small amonunt of mutant alleles. To improve this amount, the PCR products were re-amplified using as template the excised products of the mutant homoduplex and heteroduplex bands obtained at the first DGGE. This approach resulted in the enhancing of the mutant homoduplex bands whereas the heteroduplex bands remained unchanged at the second DGGE. Direct sequencing of the second round PCR clearly identified the mutation R201C in the ovarian Leydig cell tumor. We have demonstrated a relatively rapid, convenient and reliable method to improve gsp somatic mutation detection combining a second DGGE of the PCR products obtained from the heteroduplexes and mutant homoduplex bands disclosed in a first DGGE followed by direct sequencing.

736/2523

Tytuł oryginału: Mechanism of the contractile effects of galantide and Galanin (1-14) [ŕ-aminobutyric acid8]scyliorhinin-I in rat isolated fundus strips.
Autorzy: Korolkiewicz Roman P., Konstański Zdzisław, Rekowski Piotr, Szyk Agnieszka, Ruczyński Jarosław, Dąbkowski Jarosław, Ujda Marek, Korolkiewicz Konstanty Zbigniew, Petrusewicz Jacek
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR19-BR23, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Background: The study was undertaken to establish the pharmacological basis of the stimulatory activity of galantide (M15) and galanin(1-14)-ŕ-aminobutyric acid8-[Abu8])scyliorhinin-I [Scy-I] in gastric smooth muscle. Material/Methods: Isotnic contractions of the isolated, longitudinal rat gastric fundus strips were recorded. Results: Galanin, galanin(1-15)-NH2, M15 and galanin(1-14)-[Abu8]Scy-I elicited concentration-dependent contractions. Their EC50s equaled 12.95, 174, 70.06 and 187 nM respectively. Hill's coefficients for galanin and galanin(1-15)-NH2 were not different from unity, indicating an interaction of one peptide molecule with one receptor accoridng to the principles of classical receptor theory. Hill's coefficients were 0.73 and 1.56 for M15 and galanin (1-14)-[Abu8]Scy-I, respectively, a value significantly different from unity. Cold-storage denervation, tetrodotoxin-TTX (1 ćM), spantide 100 ćM) and NK1-3 receptor antagonists SR140333, 48968, 142801 (up to 10 ćM) notably diminished Mf15, galanin (1-14)-[Abu8]Scy-I, SP(5-11) and [Abu8]-Scy-I evoked contractions without affecting activities of galanin and galanin(1-15)-NH2. Additionally, cross-desensitization experiments attenuated activity of M15 and galanin(1-14)-[Abu8]Scy-I without any noticeable action on galanin or galanin(1-15)-NH2. Conclusions: The action of chimeric peptides on the smooth muscle of the rat gastrointestinal tract depended not only on the myogenic interaction of those peptides with galanin binding sites, but also on the activation of tachykinin receptors or the release of endogenous mediators from the presynaptic terminals.

737/2523

Tytuł oryginału: Soluble cytokine receptors in renal vasculitis and lupus nephritis.
Autorzy: Tesar Vladimir, Jirsa jr Milan, Zima Tomas, Kalousova Marta, Bartunkova Jirina, Stejskalova Alena, Dostal Ctibor, Zabka Jiri
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR24-BR29, tab., bibliogr. 49 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Background: The activation of various cytokines, e.g. TNFŕ, IL-1 and/or IL-6, may play an important role in the pathogenesis of renal vasculitis and lupus nephritis (LN). The systemic effect of these cytokines may be modulated by their circulating soluble receptors. The plasma levels of cytokine receptors may thus also be markers of the activation of these cytokines. Material/Methods: The plasma levels of TNFŕ, its soluble receptor p75 (sTNF-RII), IL-6, and the soluble IL-6 receptor (sIL-6R) were measured using ELISA in 17 patients with ANCA-positive renal vasculitis (12 active - ANCA-A, 7 in remission ANCA-R), 9 patients with active lupus nephritis (LN), and 5 healthy subjects. Results: Patients with LN had increased plasma levels of TNFŕ, sTNF-RII, IL-6 and sIL-6R in comparison with controls. Patients with ANCA-A also had increased plasma levels of TNFŕ, sTNF-RII adn sIL-6R in comparison with controls, but the increases in the plasma level of IL-6 was not statistically significant, due to the large standard deviation. Patients with ANCA-R had increased plasma levels of sTNF-RII in comparison to controls, but the plasma levels of TNFŕ were significantly lower in ANCA-R than in NANCA-A. While the ratio of TNFŕ to sTNF-RII was significantly lower in all groups of patients than in the acontrols, the ratio of IL-6 to sIL-6R was significantly increased only in LN in comparison to controls. Conclusions: While increased plasma levels of TNFŕ may be a nonspecific marker of the activity of ANCA-positive renal vasculitis and LN...

738/2523

Tytuł oryginału: Frequency, consequences and pharmacological treatment of gastroesophageal reflux in children with cystic fibrosis.
Autorzy: Brodzicki Jacek, Trawińska Maria, Korzon Maria
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR529-CR537, tab., bibliogr. 42 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Gastroesophageal reflux (GER) may aggravate chronic bronchopulmonary diseases. The study evaluated GER frequency and characteristics in children with Cysti Fibrosis (CF) as well as its consequences and pharmacological treatment. Material/Methods: 40 CF children aged 1.3 to 20 years were examined. The study methodology involved: medical files analysis, anamnesis, physical examination, growth status estimation, esophageal pH-metry and upper gastrointestinal tract endoscopy with histological examination of esophageal biopsies. Results: Based on pH-metry results, the diagnosis of GER was established in 22 children (55 p.c.). Mild GER (Index Reflux - IR 5-10 p.c.) was found in 12 children (54.5 p.c.), moderate GER (IR 10-20 p.c.) in 7 (31.8 p.c.) and severe GER (IR 20 p.c.) in 3 (13.6 p.c.). Ten patietns with moderate or severe GER underwent endoscopy, which revealed GER-related esophagitis in 8 cases. There was no statistical difference of GER frequency and degree according to: age, sex, growth status, presence of type DeltaF508 mutation in CFTR genome and typical GERD symptoms. According to the ESPGAN proposition, cisapride or cisapride with ranitidine medication was instituted. Treatment analysis was performed in 19 cases after successful follow-up examinations carried out three months later, indicating a significant decrease in reflux index, the longest episode duration and the numer of episodes longer than 5 minutes. Improvement of endoscopic picture was noticed after treatment. Conclusion: High frequency of gastroesophageal reflux and its consequences among...

739/2523

Tytuł oryginału: Concentration of á2-microglobulin and percentage of CD4 lymphocytes in peripheral blood in patients with chronic HCV infection during IFN-ŕ therapy.
Autorzy: Łapiński Tadeusz Wojciech, Kot Aldona, Prokopowicz Danuta
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CR538-CR542, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: We evaluated the serum á2-MG concentration and CD4 lymphocytes in the blood of HCV infected patietns during IFN-ŕ therapy, searching for a correlation between á2-MG concentration. CD4 lymphocytes, and tehrpay effectiveness, as well as morphological changes in the liver. Material/Methods: 24 patietns with chronic HCV infection were treated with IFN-ŕ2a. The serum á2-MC concentration was measured with the use of a method based on teh fluorescent modification of the immunoenzymatic technique. The percentage of T CD4 lymphocytes in the blood was measured by direct immunofluorescence using monoclonal antibodies. Results: The number of CD4 lymphocytes in teh blood was lower in the HCV infected patients (864/ml; preferred values from 1,300 to 2,100/ml), the á2-MG concnetration was elevated (2.37 mg/dl) in comparison to the preferred values (1.52 mg/dl; p 0.05). IFN-ŕ therapy caused an increase in the á2-MG. The highest increase was observed among patients who did not eliminate the virus (from 2.39 to 4.10 mg/dl). In the initial period of interferon therapy an increase was observed (from 729 to 1082/ml) in the number of CD4 lymphocytes among those patients who eliminated the virus and a decrease (from 947 to 853/ml) in the patietns who were not treated successfully. Conclusions: A significant increase in á2-MG during interferon therapy in patietns with chronic HCV infection is a predictor of poor outcome. An increase in the number of CD4 lymphocytes in the initial phase of treatment suggests a positive outcome.

740/2523

Tytuł oryginału: Cheyne-Stokes respiration during sleep: a possible effect of body position.
Autorzy: Oksenberg Arie, Arons Elena, Snir David, Radwan Henryk, Soroker Nachum
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CS61-CS65, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: Cheyne-Stokes Respiration (CSR) is a common finding in Chronic Heart Failure and Stroke patients. The body position effect during sleep on obstructive breathing abnormalities is well known. However, the effect of body position during sleep on breathing abnormalities of central type like CSR has not been well documented. Material/Methods: Six sleep studies (two complete Polysomnographic (PSG) evaluations and four Pulse Oximetry recordings (PO)), were carried out in a 57-year-old female patient with a recent Cerebro Vascular Accident (CVA who had both Obstructive Sleep Apnea (OSA) and CSR. Results: The first PSG was carried out tow months post-stroke an drevealed a severe, continuous CSR pattern during Non Rapid Eye Movements (NREM) sleep (mainly with central apneas), and Obstructive Sleep Apnea (OSA) during Rapid Eye Movements (REM) sleep, independent of body positon: Supine Respiratory Disturbance Index (SRDI) = 85.2 and Lateral RDI (LRDI) = 95.4. A second PSG was performed three months later after an overall clinical improvement and showed a complete disappearance of CSR during NREM sleep and OSA during REM sleep in her lateral posture (LRDI = 0), while the RDI in the supine posture was only slightly improved (SRDI = 73.2). The CSR pattern was less severe and was characterized mainly by central hypopneas. Two PO recordings between the PSG studies showed similar improvement trends. Two additional PO recordings, two and three weeks after the last PSG (the first one with the patient lying supine and the second one with the patient lying on her side through-out the night),...

741/2523

Tytuł oryginału: Psychiatric symptoms in a patient with the clincial features of MELAS.
Autorzy: Kiejna Andrzej, DiMauro Salvatore, Adamowski Tomasz, Rymaszewska Joanna, Leszek Jerzy, Pąchalska Maria
Źródło: Med. Sci. Monitor 2002: 8 (7) s.CS66-CS72, il., tab., bibliogr 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: This article presents a case study of a 32-year-old Polish male patient clincally diagnosed with the MELAS syndrome, the second such patient reported in this country. Case report: The patient presents with a long history of neurological episodes, consisting of acute neurological deficitis suggestive of CVA with no evidence of focal lesion and very rapid remission of symptoms. These episodes began when the patient was 19 years old and have recurred since that time at intervals of 1-5 years. A recent psychiatric episode with unusual visual hallucinations brought the case to the attention of the present authors, who initiated further diagnostic testing. A muscle biopsy revealed ragged red fibers. Conclusions: The features in this case meet the diagnostic criteria for a clincial diagnosis of MELAS, pending confirmation of a pathogenic mutation.

742/2523

Tytuł oryginału: Thermal effect of intravascular MR imaging using an MR imaging-guidewire: an in vivo laboratory and histopahtolgical evaluation.
Autorzy: Yang Xiaoming, Yeung Christopher J., Ji Hongxiu, Serfaty Jean-Michel, Atalar Ergin
Źródło: Med. Sci. Monitor 2002: 8 (7) s.MT113-MT117, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: Background: Intravascular magnetic resonance (MR) imaging to guide interventional procedures is a rapidly growing field. A primary concern with these new techniues is their thermal safety. The purpose of this study was to evaluate, in vivo, the thermal effect of an MR imagingguidewire (MRIG) for intravascular MR imaging (IV MRI). Material/Methods: Two indications of potentially adverse local heating were investigated: blood coagulation disorders and pathologica changes in target vessels. Experiments were performed on ten rabbits with a 1.5 T MR scanner. Using a 0.64-mm MRIG as the RF receiver, we imaged the target aorta using a fast spin-echo pulse sequence with an average spcific absorption rate (SAR) of 0.6 W/kg. The total MR imaging time was approximately 70 minutes. Results: There were no abnormal value changes of the coagulation factors between pre- and post-IV MRI, no clinical manifestations of blood coagulation disorders, and, histopathologically, no thermal damage in target vessels. Conclusions: This study demonstrates, from a pathophysiological point of view, the potenial safe use of the MR imaging-guidewire for intravascular MR imaging. Further study is required to precisely define the boundaries of these safe operating parameters.

743/2523

Tytuł oryginału: Robotically-assisted coronary artery surgery with and without cardiopulmonary bypass - from first clinical use to endoscopic operation.
Autorzy: Detter Christian, Boehm Dieter H., Reichenspurner Hermann, Deuse Tobias, Arnold Martin, Reichart Bruno
Źródło: Med. Sci. Monitor 2002: 8 (7) s.MT118-MT123, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Recently, the ZEUSTM Robotic Surgical System has been introduced to increase the precision of endoscopic cardiac surgery. This study investigated its clinical use for endoscopic coronary artery bypass grafting. Material/Methods: Between 1998 and 2001, 41 patients with single and multivessel disease were operated on using the ZEUSTM system. The robotic system was introduced step by step into clinical practice. Initially, the system was used only for endoscopic internal mammary artery (IMA) harvest (n = 12), later for coronary anastomoses on the arrested (n - 13) or beating heart after median sternotomy (n = 6), and finally for endoscopic coronary bypass grafting on either the arrested (n = 2) or beating heart (n - 8). Results: Endoscopic IMA harvest ranged from 48 to 110 min and was completed in all cases. In the sternotomy group, the robotic anastomosis time averaged 21 min on the arrested and 25 min on the beating heart, respectively (n.s.). In the endoscopic cases, the average time for endoscopic anastomosis was 41 min on the arrested and 36.5 min on the betaing heart (n.s.), with an overall duration of surgery between 4.0 and 8.0 hours. One endoscopic case was intraoperatively converted to a MIDCAB procedure with manual anastomosis. THe total patency rate of all graft anasomoses, confirmed by early postoperative angiographic control, was 97 p.c. One patient underwent reopartion with an uneventful postoperative course. Conclusions: The present study demonstrates the feasibility of endoscopic coronary revascularization using a computer-assisted surgical robotic system on the arrested and beating heart in selected patients.

744/2523

Tytuł oryginału: Towards a semantic medical Web: HealthCyberMap's tool for building an RDF metadata base of health information resources based on the Qualified Dublin Core Metadata Set.
Autorzy: Boulos Maged N. Kamel, Roudsari Abdul V., Carson Ewart R.
Źródło: Med. Sci. Monitor 2002: 8 (7) s.MT124-MT136, il., bibliogr. 52 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Background: HealthCyberMap (http://healthcybermap.semanticweb.org/) aims at mapping Internet health information resources in novel ways for enhanced retrieval and navigation. This is achieved by collecting appropriate resouce metadata in an unambiguous form that preserves sematics. Material/Methods: We modelled a qualified Dublin Core (DC) metadata set ontology with extra elements for resource quality and geographical provenance in Protg-2000. A metadata collection form helps acquiring resource instance data within Protg. The DC subject field is populated with UMLS terms directly imported from UMLS Knowledge SOurce Server using UMLS tab, a Protg-2000 plug-in. The project is saved in RDFS/RDF. Results: The ontology and associated form serve as a free tool for building and maintaining and RDF medical resource metadata base. The UMLS tab enables browsing and searching for concepts that best describe a resource, and importing them to DC subject fields. The resultant metadata base can be used with a search and inference engine, and have textual and/or visual navigation interface(s) applied to it, to ultimately build a medical Semantic Web portal. different ways of exploiting Protg-2000 RDF output are discussed. Conclusion: By making the context and semantics of resources, not merely their raw text and formating, amenable to computer 'understanding', we can build a Semantic Web that is more useful to humans than the current Web. This requires proper use of metadata and ontologies. Clinical codes can reliably describe the subjects of medical resources, establish the semantic relation-ships (as defined by underlying coding scheme) between related resources, and autmoate their topical categorisation.

745/2523

Tytuł oryginału: Microcirculation in the diabetic foot as measured by a multichannel laser Doppler instrument.
Autorzy: Karnafel Waldemar, Juskowa Joanna, Maniewski Roman, Liebert Adam, Jasik Mariusz, Zbieć Anna
Źródło: Med. Sci. Monitor 2002: 8 (7) s.MT137-MT144, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of this study was to investigate microvascular perfusion in insulin-dependent diabetic patients at various locations on the foot, and to determine which part of the foot is most sensitive to microangiopathic changes. All the parameters of postocclusive reactive hyperemia calculated from multichannel laser Doppler recordings were also evaluated to find the most valuable measure for diabetes examination. Material/Methods: Our study involved 65 subjects divided into four subgroups: male and female controls, and male and female IDDM patients without overt complications. The measurements were performed with a multichannel laser Doppler perfusion monitor using surface probes located in the distal parts of the lower limbs. The occlusion test was performed using a cuff located on the limb above the knee. Multivariate discriminatory analysis was used to evaluate the data. Results: The most valuable data were obtained by recordings from the laser-Doppler probes located on the hallex and the base of the little toe. The maximum hyperemic response for both sex subgroups was significantly lower in the diabetic patients. The time to peak flow was higher in male diabetics. The half-time for hyperemia was significantly longer in the male diabetic patients. Cocnlusions: The females showed smaller changes in foot perfusion than the males, probably due to protection by estrogens. The best locations for perfusion measurement are the most distal, especially the hallex and the base of the little toe. The most valuable parameters of postocclusive hyperemia were maximum response, time to peak flow, and half-time of hyperemia.

746/2523

Tytuł oryginału: Mechanisms of knowledge learning and acquisition.
Autorzy: Salamon Elliott
Źródło: Med. Sci. Monitor 2002: 8 (7) s.RA133-RA139, il., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The mechanism by which knowledge enters into memory has been a source of debate for some time. Theorists have proposed several models that aim at explaining the sequence of events from the perception of a stimulus, toits entrance into long-term storage. Much of this work was prompted by early research into the nuances of classical conditioning where it was first firmly established that organisms are capable of detecting covariations of stimuli within their environment. Subsequent work in the field has shown that these covariations form the basis for the mental representation of our surroundings, as well as the basis of learning. Work within the field of classical conditioning, along with the advance of computer technology and neuroscience has made these architectural models even more complex. Furthermore, experiments designed to support some of these proposed models have revealed that there are several conditions that can either aid or inhibit the transition of information into permanent storage. In this review we explore a number of these models, along with some classic critiques that have been levied against them. We also provide some history into the form of knowledge, termed 'implicit knowledge', as well as some of the proposed mechanisms of limplicit knowledge acquisition. We conclude by exploring the newly proposed theoretical framework within which implicit learning theory operates.

747/2523

Tytuł oryginału: The fragile histidine triad gene and breast cancer.
Autorzy: Yang Qifeng, Yoshimura Goro, Sakurai Takeo, Kakudo Kennichi
Źródło: Med. Sci. Monitor 2002: 8 (7) s.RA140-RA144, bibliogr. 46 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The Fragile Histidine Triad (FHIT) Gene, encompassing the FRA3B fragile site at chromosome 3p14.2, is a tumor suppressor gene involved in different tumor types. Abnormalities of the FHIT locus were found in many established cancer cell lines and tumors including breast cancer. In sporadic breast cancer, loss of heterozygosity within the FHIT gene has been observed at different frequencies and has been correlated with tumor progression. Aberrant FHIT transcripts have been reported in breast cancer. Furthermore, Fhit protein loss is correlated with prognosis. We summarized the research advances of FHIT genetic, epigenetic change and aberrant Fhit protein expression in breast cancer. Fhit protein may be a novel prognostic factor for breast cancer. FHIT gene therapy may potentially be clinically be clinically useful for treatment of breast cancer, suggesting further research involving FHIT introduction should be performed in breast cancer.

748/2523

Tytuł oryginału: TGF-á (transforming growth factor-á) in chronic inflammatory conditions - a new diagnostic and prognostic marker?
Autorzy: Marek Andrzej, Brodzicki Jacek, Liberek Anna, Korzon Maria
Źródło: Med. Sci. Monitor 2002: 8 (7) s.RA145-RA151, il., bibliogr. 58 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: TGF-á is a cytokine with varied properties and pleiotropic activity. It is released in an inactive form. To exhibit its biological activity, it requires binding to extracellular matrix proteins and, after that, proteolytic elimination of LAP (Latent Associated Protein) and LTBP (Latent TGF-á Binding Protein). The process involves, among others, tissue transglutaminase, thrombin and plasmin. By stimulation of specific receptors, it influences transcription of some genes and translation of formed mRNA. Locally, it demonstrates proinflammatory properties whereas systemically, it has primarily a potent immunosuppressive effect. TGF-á, by affecting proliferation, differentiation and migration of cells, as well as stmulation of extracellular matrix protein production, plays an important role in tissue regeneration and remodeling, but also in fibrosis. TGF-á is also indispensable to maintain immune homeostasis of the organism. Reduced TGF-á activity is considered to be responsible for development of autoimmune disorders in the course of several pathologic conditions. This cytokine plays an important role in the pathogenesis of chronic inflammatory processes taking place, among others, in inflammatory bowel diseases (IBD) and chronic hepatitis B and C. The paper presents a review of literature concerning diagnostic and prognostic value of TGF-á level determinations in blood and tissue bioptates of patients with chronic non-specific enteritis and chronic hepatitis B and C.

749/2523

Tytuł oryginału: Pharmacogenomics.
Autorzy: Tribut Olivier, Lessard Yvon, Reymann Jan-Michel, Allain Herv, Bentu-Ferrer Danile
Źródło: Med. Sci. Monitor 2002: 8 (7) s.RA152-RA163, il., tab., bibliogr. 103 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Pharmacogenomics, a revolutionary chapter in the history of pharmacology, has received new impetus from the development and accessibility of molecular biotechnologies, notably DNA chips. The longstanding notion of responders/non-responders has given way to a more organic approach, where idiosyncrasy becomes an obsolete concept. This is a major step towards predictive, individualized medicine. In this review, several applications of pharmacogenomics are considered. Genetic polymorphisms of metabolization reactions, mainly with cytochrome P450, explain most of the cases described today. More fundamental and innovative studies have tried to link the structure of receptors or transporters and drug response. A leading topic in neuropsychopharmacology is the relation between the polymorphism of dopaminergic receptors and the efficacy of, or adverse reaction to, neuroleptics. In asthma, the structure of the á2-adrenergic receptor has been associated with response to treatment. Intrinsic genetic predisposition also plays an important role in cardiovascular diseases, and the role of ion channel mutations will be discussed. Research in oncological molecular epidemiology has explored the connection between the predisposition to certain cancers and specific enzymatic equipment hindering the detoxification of potentially carcinogenic exogenous compounds, or, on the contrary, promoting metabolic activation implicated in the formation of reactive compounds. The search for determinants of addictive behavior is another vast field of pharmacogenomics...

750/2523

Tytuł oryginału: Electron microscope investigation of the role of Chlamydia sp. in the process of rebuilding the arterial wall. Neoangiogenesis in atherosclerotic plaques in human cervical artery walls.
Autorzy: Walski Michał, Łazowski Tomasz, Szosek Mieczysław, Opolski Grzegorz, Celary-Walska Renata, Andruszkiewicz Paweł, Ryba Mirosław
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR30-BR38, il., bibliogr. 40 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: The inflammatory process has an essential impact on the development of atherosclerosis. There mechanisms are mentioned.: 1) possibility of direct development of Chlamydia infection in the vicinity of the blood vessel wall; 2) impact of persistent or recurrent Chlamydia infection on the increased blood concentration of risk factors for atherosclerosis; 3) an autoimmunological reaction. Material/methods: Electron microscope examinations were performed on specimens from atherosclerotic lesions of the interior cervical arteries, collected from patients who had undergone endarterectomy. The material came from 8 patients (age from 58 to 72). The specimens were fixed for electron microscopy, and after dehydration were immersed in Spurr resin. Ultrathin slices were examined under a transmission electron micoroscope. Results: In the successive tested layers nearest the lumen of the vessel we found erythrocytic elements, fibrin, and lipid membranes. In deeper layers there were lymphocytic cells, monocytes, and macrophages loaded with phagocyted lipid material. Under this layer we found in some specimens a coating which had undergone mineralization: calcium structures and cholesterol were overlaid on a proteoglycanate base. Smooth muscles cells had undergone the heaviest proliferation among the cells on artery wall. In the tested material we detected diversified morphological forms of Chlamydia sp. Particular attetnion should be drawn to the appearance of very young vessel forms, which suggests a process of angiogenesis in the atherosclerotic plaques. Conclusions: We found that one of the pathogens that may lead ot atherosclerotic lesions is chalmydia wp. The process of atherogenesis in cervical arteries is accomanied by angiogenetic processes.

751/2523

Tytuł oryginału: Usefulness of immunohistochemical testing and electron microscopy in the diagnosis of embryonal rhabdomyosarcoma.
Autorzy: Salwa-Żurawska Wiesława, Biczysko Wiesława, Woźniak Aldona, Janicka-Jedyńska Małgorzata, Trejster Ewa
Źródło: Med. Sci. Monitor 2002: 8 (1) s.BR39-BR46, il., tab., bibliogr. 41 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The article presents the results of histological, immunohistochemical, and ultrastructural examinations of embryonal rhabdomyosarcoma. Material/methods: 20 tumors were tested histologically, 13 immunohistochemically, and 5 under electron microscopy. Results: We found that in cases when the entire tumor or large specimens were obtained for estimation, it was possible by examining many areas of the neoplasm to establish a diagnosis on the basis of testing paraffined sections, routinely HE stained. Our evaluation of the value of certain additional histological methods indicated only slight diagnostic usefulness for tumors of this kind. Among the immunohistochemical methods we evaluated, the desmin assay had the greatest practical applicability, due to the fact that this antigen is also expressed in highly immature cells; in second place was myoglobin. Differences are pointed out in the results obtained by assaying myoglobin using DAB and the DAKO kit. Ultrastructural examinations revealed myofilaments of myosin and actin in all types of cells, including immature cells. Particular attention is drawn to the possibility of using material fixed in formalin for ultrastructural examination. The preservation of intact myofilaments in mateiral prepared in this way constitutes an essential diagnostic aid. Conclusions: In diagnosing embryonal rhabdomyosarcoma the most useful examination, apart from histological examination, is electron microscopy, followed by immunomorphological examination.

752/2523

Tytuł oryginału: Ser9Gly polymorphism in the dopamine D3 receptor gene is not associated with essential hypertension in the Japanese.
Autorzy: Soma Masayoshi, Nakayama Tomohiro, Rahmutula Dolkun, Uwabo Jiro, Sato Mikano, Kunimoto Masako, Aoi Noriko, Kosuge Kotoko, Kanmatsuse Katsuo
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR1-CR4, tab., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Background: The Dopamine D3 receptor (DRD3) gene is thought to be involved in essential hypertension (EH) because dopamine inhibits renin secretion via this receptor and because disruption of the DRD3 gene increases blood pressure in mice. EH is a complex, polygenetic disease. Association studies using the candidate gene approach may provide important clues regarding the etiology of hypertension and define a basis for further genetic investigation. Therefore we examined the association between the Ser9Gly polymorphism in the DRD3 gene and EH. Material/methods: One hundred eighty-one patients with EH and 181 age-matched subjects with normal blood pressure were enrolled. Genomic DNA was extracted from peripheral blood leukocytes. Polymerase chain reaction (PCR) was used to amplify the Ser9Gly polymorphic site in the DRD3 gene, and restriction fragment length polymorphism (RFLP) analysis of the PCR product was used to score the A and G alleles. Plasma renin acitvity and plasma aldosterone concentration were measured in untreated EH subjects. Results: The genotype distribution was in Hardy-Weinberg equilibrium, and was not significantly different between the NT and EH groups. The frequencies of A and G alleles were 0.674 (244/362) adn 0.326 (118/362) for the NT group and 0.688 (249/362) and 0.312 (113/362) for the EH group, respectively, and did not differ significantly between the two groups. The genotype did not influence the plasma renin activity and aldosterone concentration in untreated EH patients. Conclusions: The Ser9Gly polymorphism in the DRD3 gene are not associated with EH. However, our negative result does not exclude the possibility of another variant elsewhere in or near the DRD3 gene in EH.

753/2523

Tytuł oryginału: Effect of tissue injury on D-Dimer levels: a prospective study in trauma patients.
Autorzy: Johna Samir, O'Callaghan Thomas, Cemaj Samuel, Catalano Richard
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR5-CR8, il., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: D-Dimer measurement has been used as a simple, non-invasive test to rule out thromboembolic phenomena in patients at risk for deep venous thrombosis (DVT) and/or pulmonary embolism (PE). Elevated D-Dimer level caused by tissue injury is believed to show a trend for gradual decrease to normal within the first three days after trauma. Material/methods: To study the effect of tissue injury on D-Dimer levels, we conducted a prospective measurement of D-Dimer levels in severely traumatized, high-risk patients for DVT or PE, starting within 24 hours after admission until disposition of the patient or to a total of 14 days of hospitalization. Patients were observed clinically for development of thromboembolic phenomena, and were subjected to weekly surveillance using duplex scan of teh lower exatremities. Additional testing was done if requested by the attending trauma surgeon. Results: A total of 21 patients wer enrolled in the study. There were 17 males, and 4 females. Patients had a mean age of (42) with a range of (17 - 79), and a mean ISS score of (20) with a range of (4 - 50). Seven patients completed 3 - 9 days of testing. Fourteen patients had more than 10 days of testing. Nine patients completed 14 days of testing. In all patients, tissue injury resulted in increased levels of D-Dimer above a threshold (500 ng/ml), below which DVT or PE can be ruled out. The increased levels failed to normalize even when testing was continued for 14 days. Conclusions: In our study, the increased D-Dimer levels induced by tissue injury failed to show a trend of gradual return to normal within...

754/2523

Tytuł oryginału: Activity of serum lipase [EC 3.1.1.3] and the diversity of serum lipid profile in psoriasis.
Autorzy: Pietrzak Aldona, Lecewicz-Toruń Barbara
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR9-CR13, tab.,bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: In the course of psoriasis the loss of scales from the skin surface is observed. This may be related to lipid disorders in epidermis and in blood serum. The aim of the study was to find out if serum lipid profile disturbances occur in psoriatic patients. Material/methods: We studied 26 male and 15 female normolipidemic psoriatic patients, and 29 male and 28 female healthy normolipidemic volunteers. The concentration of serum pancreatic lipase, total cholesterol, HDL cholesterol, LDL cholesterol, total phospholipids, HDL phospholipids, LDL phospholipids and triglycerides were measured with enzymatic methods. Results: The study of male patients demonstrated a statistically significant decrease in HDL cholesterol concentration and statistically signifcant increase in triglyceride concentration. In psoriatic females, we observed a statistically significant decrease in HDL cholesterol concentration, a statistically significant decrease in HDL phospholipid concentration, and a statistically significant increase in pancratic lipase concentration. In addition, the pancereatic lipase concentration increased insignificantly in the male group. We found lipid profile disturbances in the groups of psoriatic patients, in comparison with the age-, sex-, and BMI-matched control groups. Conclusions: The obtained results suggest the necessity of careful administration of medicines to psoriatic patients suffering from systsemic diseases and treated with drugs affecting lipid metabolism.

755/2523

Tytuł oryginału: An evaluation of the impact of supplementation with antioxidant vitamins on oxidation stress parameters in children with chronic recurrent gastroduodenitis.
Autorzy: Bała Grażyna, Czerwionka-Szaflarska Mieczysława, Drewa Gerard, Mierzwa Grażyna
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR14-CR18, tab., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of our research was to evaluate the impact of supplementation with antioxidative vitamins in children with chronic recurrent gastroduodenitis on the concentration of malondialdehyde (MDA) in plasma and erythrocytes, and on acitivity of the antioxidant enzymes: dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Material/methods: We studied 19 children (age from 13 to 16), diagnosed with chronic recurrent gastroduodenitis. The concentration of MDA was measured by Buege and Aust method, while the antioxidant enzyme activity was measured before and after a two-month course of supplementation with vitamins C and E: SOD by the Misra and Fridovich method, CAT by the Bears and Seizer method, and GSH-Px by the Paglia and Valentine method. Results: The average concentration of MDA in erythrocytes in the studied children was significantly higher (38.59 nM/gHb) than non control group (24.07 nM/gHb), but after supplementation was significantly reduced (p 0.05). The average SOD activity in the experimental group was significantly lower both before (885 U/gHb) and after supplementation (923.3 U/gHb) than in the control group (1240 U/gHb). The activity of the remaining enzymes did not differ significantly in experimental and control groups. Conclusions: The increased erythrocyte concentration of MDA in children with chronic recurrent gastroduodenitis may point to the generation of active forms of oxygen due to the low activity of the anti-oxidant enzyme SOD. Supplementation with the antioxidant vitamins C and E in children with a low antioxidant potenital and chronic recurrent gastroduodenitis reduces the generation of free oxygen radicals.

756/2523

Tytuł oryginału: Evaluation of bladder instability in children with recurrent urinary tract infections.
Autorzy: Winiecka Wiesława, Zoch-Zwierz Walentyna, Wasilewska Anna, Wierciński Ryszard, Tomaszewska Barbara, Korzeniecka-Kozerska Agata, Porowski Tadeusz
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR19-CR23, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Impairment in the functions of the lower urinary tract can be the cause of recurrent urinary tract infections (UTI) and vesicoureteral reflux (VUR) in children. The purpose of our research was to evalute the frequency of occurrence of bladder instability in children with UTI. Material/methods: The research involved 114 children (21 boys, 93 girls), ranging in age from 5 to 16. Group I consisted of 61 children with a history of recurrent UTI, while Gorup II included 53 children with recurrent UTI and VUR. Urodynamic tests (uroflowmetry and cystometry) were done on all the children, while in selected cases profilometry was also performed, using a Duet apparatus (Dantec Medical A/S). Results: Abonrmal functioning of the lower urinary tract was found in 45 children (74 p.c.) from Group I and 44 children (84 p.c.) grom Gorup II. The most common dysfunction was instability of the detrusor muscle, which was found in 52 children (45 p.c.), including 23 (38 p.c.) from Group I and 29 (55 p.c.) from Group II. In 19 children detrusor instability was accompanied by reduced bladder volume, and in 8 cases there as a lack of detrusor-sphincter coordination. In both groups ca. 20 p.c. of the children did not present with symptoms indicating urination dysfunctions. Ca. 80 p.c. reported various symptoms, of which the most common were nocturnal wetting and urinary urgency. In half of the children from Group I and one-fourth of the children from Group II there were several co-occurring symptoms; nocturnal and diurnal wetting, pollakiruria, and urinary urgency, or all three symptoms simultaneously. Conclusions: The most common disturbance of lower urinary tract functioning in these children with recurrent UTI was instaability of the detrusor muscle, which occurred more often in children with VUR.

757/2523

Tytuł oryginału: Mathematical modelling in blood purification processes.
Autorzy: Weryński Andrzej, Waniewski Jacek
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.69-84, il., bibliogr. 32 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The application of the one-dimensional theory of mass transport to the derivation of mathematical formulas for clearances of a variety of membrane mass exchangers, namely hemodialyzers, hemofilters, plasma separators, and the cascade filtration procedure, has been reviewed. The applied theory predicts that clearances depend approximately on fluid inlet rates but not no concentrations, and with constant fluid flow rates, clearances are constant. Also, the applications of device clearance in kinetic modelling has been discussed.

758/2523

Tytuł oryginału: Miniaturised semiconductor electrochemical biosensors.
Autorzy: Torbicz Władysław, Pijanowska Dorota
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.85-98, bibliogr. 49 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A review of own reserach works at the IBBE PAS on semiconductor biosensors of urea, glucose, pesticides, triglicerides and selected antigens in liquids, including biological fluids, on a background of the international state of art are presented in the paper. In the all above-mentioned biosensors, enzymatic reactions of substrate hydrolysis are used to receive the products, which detection would be possible by own construciton chemically sensitive transducers of the ISFET type. Methods of immobilisation of enzymes in different matrices are presnted. Parameters of these biosensors are given.

759/2523

Tytuł oryginału: Optical methods for tissue perfusion and oxygenation measurements.
Autorzy: Maniewski Roman, Liebert Adam
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.99-112, il., bibliogr. 41 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Optical methods from the infrared range, especially Laser-Doppler flowmetry and near infrared spectroscopy offer a new non-invasive, real-time technique for monitoring of blood perfusion and oxygenation in living tissue. In spite of some instrumental problems e.g. relative calibration, unknown sampling measurement depth, these methods are already used in clincial studies. In the paper the methods principle, technical development and selected clinical applications have been described.

760/2523

Tytuł oryginału: Past and present of hall effect applications.
Autorzy: Maniewski Roman, Zawicki Ignacy
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.113-128, il., bibliogr. 22 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The article presents the review of the Hall-effect applications in measurement technique, especially the research in this field carried out some time ago at the Institute of Biocybernetics and Biomedical Engineering PAS. The idea and constructions of many Hall-effect devices e.g. teslometers, electric power transducers, mechano-electric transducers for displacement and vibration measurements, seismograph and acceleration transducer are described in the article. Special attention was paid to biomedical applications of Hall-generators like mechanocardiograms and tremor recording as well as to the present status of the Hall-effect applications in measurements and automatic control.

761/2523

Tytuł oryginału: Tissue insulin sensitivity measurements in men. Quality factors and performance determinants for successful clamp experiments. Desing and development of the TISS system and its clinical verifications.
Autorzy: Wójcicki Jan M., Ładyżyński Piotr, Foltyński Piotr, Mikulicz Hubert, Blachowicz Jacek
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.129-144, il., tab., bibliogr. 6 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

ludzie

Streszczenie angielskie: Insulin sensitivity (IS) is one of the important diagnostic parameters used in treatments of diabetes, hypertension and other diseases as well as during evaluation of new drug therapies. Euglycaemic glucose clamp is the most accurate method of the IS measurement. Effectiveness of this procedure depends on accuracy of the tissue glucose utilization measurements (GU) and proper realization of the insulin infusion. Application of the accurate glucose control algorithm, sampling site, sampling frequency, duration of the procedure are also very important factors. Possible technical realization includes manual, semi-automatic and automatic modes. Recently the computerized semi-automatic system realizing this type of measurement has been designed and developed in the IBBE PAS. A developed system consist of control unit, infusion unit, glycaemia measurement unit and printer. Endogenous glucose secretion is suppressed by insulin infusion, which is determined according to individual patients parameters. The newest version of the measurement procedure allows to realize euglycaemic or normoglycaemic clamp studies. TISS system is inexpensive and easily operated by one person from middle medical staff. Till now GU measurements using TISS system were performed on over 200 patients in 4 Polish clincial centers. Developed and clinically tested TISS system seems to provide satisfying quality of the GU measurement in men.

762/2523

Tytuł oryginału: Cardiovascular, respiratory and veno-lymphatic assistance. Modelling and simulation.
Autorzy: Górczyńska Krystyna, Darowski Marek, Kozarski Maciej
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.135-175, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: In the paper some problems concerning investigations conducted at Bioflows Department of the Institute of Biocybernetics and Biomedical Engineering on cardiovascular, respiratory and veno-lymphatic assistance have been discussed. The investigations were mainly focused on modelling of cardiovascular and respiratory systems, cardiopulmonary interaction and veno-lymphatic non-invasive support. The results of physical and computer simulation of cardiopulmonary assistance and clinical results of veno-lymphatic support studies have been presented.

763/2523

Tytuł oryginału: From pattern recognition to image interpretation.
Autorzy: Kulikowski Juliusz L.
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.177-197, il., bibliogr. 27 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In this paper selcted problems concerning computer-aided image processing in biomedical applications are described. The problems are related to a multi-year activity in this domain of the Department of Biomedical Information Processing Methods of the Institute of Biocybernetics and Biomedical Engineering PAS in Warsaw. In particular, an extension of the k-NN pattern recognition algorithm on the case of non-metric observation spaces is described. An example of a composite pattern recognition based on morphological parameters is presented. A concept of relations recognition as an extension of pattern recognition is also shortly described. In the last section some remarks concerning iamge interpretation methods have been given.

764/2523

Tytuł oryginału: Selecte problems in bionics.
Autorzy: Zmysłowski Wojciech
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.199-206, bibliogr. 10 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Selected problems concerning the studies in the field of bionics have been reviewed. It have been shown that this discipline of science has an important impact on the development of studies in other disciplines and on the development of the innovative technologies. Particular attention was paid to the technical studies on information processing and control and biological studies on the mechanisms of physiological processes.

765/2523

Tytuł oryginału: Practical approaches to statistical pattern recognition.
Autorzy: Jóźwik Adam
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.207-221, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The paper describes new approaches to statistical pattern recognition. All presented methods are based on a distance function. The properties of these methods and their usefulness are illustrated on real problems. Some tasks with small and very large training sets are described to shown and effectiveness of the proposed approaches. There is no one universal method that would be satisfactory for all object classification problems. That's why several methods have been demonstrated.

766/2523

Tytuł oryginału: Ranked designing of prognostic neural models for survival data.
Autorzy: Bobrowski Leon, Krętowska Małgorzata
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.223-239, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 306,224

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The procedure of designing the non-linear dependencies of survival time on a family of covariates is described in the paper. This dependence is treated as a prognostic model. The first stage of this procedure involves designing such layers of formal neurons with are ranked with respect to selected subsets of censored data. The model based on the hierarchical networks of formal neurons results in the piecewise constant dependence of the survival time on the covariates. The replacement of the formal neurons results in the piecewise constant dependence of the survival time on the covariates. The replacement of the formal neurons by the logistic ones made it possible transform this dependence into differentiable, regression-type function.

767/2523

Tytuł oryginału: Monitoring pilot's eye movements during the combat flight - the white box.
Autorzy: Ober Jan Krzysztof, Ober Jan Jakub, Malawski Marek, Skibniewski Wojciech, Przedpelska-Ober Elżbieta, Hryniewiecki Jerzy
Źródło: Biocybern. Biomed. Eng. 2002: 22 (2/3) s.241-264, il., bibliogr. 16 poz.
Sygnatura GBL: 306,224

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

ludzie

Streszczenie angielskie: Modern fighter pilots are often exposed to excessive accelerations. The resulting brain hipoxia can lead to G-LOC (G-induced loss of consciousness). Authors discuses the effects of microcirculatory adaptation released by the push-pull condition (rapid change from minus to plus Gz). The memory effect of vaso-constriction due to - Gz can be responsible for intermittent blocking of the compensatory vasodilatation and diminishing the +Gz tolerance of pilots tested on the centrifuge. Authors consider the push-pull condition to be analogous to the rapid blocking of the brain perfusion caused by the high plus Gz onset rates (1,G/s). Using the visual symptoms (narrowing of the visual field) is not longer adequate for monitoring the centrifuge push-pull training. Authors discuses the possibility of suing the saccadic eye movements as being the most adequate physiological signal to monitor the pilot's consciousness. Measuring pilots eye movements requires to develop a miniatrue complex motion transducer which can be used during centrifuge training as well as during the flight (the White Box). Problems encounterd during the system development are discussed.

768/2523

Tytuł oryginału: Evaluation of several methods used in anatomical investigations of the blood and lymphatic vessels.
Autorzy: Jędrzejewski Kazimierz S., Cendrowska Ilona, Okraszewska Ewa
Źródło: Folia Morphol. 2002: 61 (2) s.63-69, il., bibliogr. 15 poz.
Sygnatura GBL: 301,720

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The aim of this review is to describe the advantages and limitations of several methods used in anatomical investigations of intravisceral blood and lymphatic networks. The microangiographic methods as well as corrosion methods are described. In conclusion the authors as well as corrosion methods are described. In cocnlusion the authors confirmed that the most useful way for exploration of the blood and lymphatic vessels is to prepare corrosion casts. This paper focuses on the scanning electron microscopic examination of vascular corrosion casts. This method allows the examination of the three-dimensional organisation of vessels, including the blood and lymphatic capillaries. Imprints of endothelial cell nuclei can be observed on the surface of the blood and lymphatic vessels.

769/2523

Tytuł oryginału: Anatomical study of lumbar spine innervation.
Autorzy: Higuchi Katsura, Sato Tatsuo
Źródło: Folia Morphol. 2002: 61 (2) s.71-79, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: To precisely evaluate low back pain, identification of the detailed innervation of the lumbar spine is necessary. On twenty-five sides of adult cadavers we investigated various patterns of rami communicantes (RC) and their relationship to the psoas major muscle (PM). In ten sides, we focused our dissection on the minute nerve supply of the anterior (ALL) and posterior longitudinal ligaments (PLL), vertebral bodies and the intervertebral discs (IVD). According to the mode of piercing PM, two types of RC were observed: superficial oblique rami (SOR) and deep transverse rami (DTR). SOR ran obliquely between supreficial heads of PM, connecting sympathetic trunk (ST) and T12-l2 (3) spinal nerves non-segmentally. DTR ran segmentally close to the vertebral bodies and were situated deep to the pM slips. On the lateral side of the lumbar spine, the vertebral bodies and IVD received branches from DTR and ventral rami segmentally, as well as branches from the sympathetic trunk (ST) and, in the upper lumbar region, SOR non-segmentally. On the anterior aspect of the lumbar spine, ALL received branches from ST and splanchnic nerves non-segmentally. Within the vertebral canal, the posterior aspect of IVD and PLL received the sinu-vertebral nerves originating from DTR. These findings suggest the coexistence of two different types of innervation: one originating directly from the spinal nerve segmentally, and one reaching vertebral structures via the sympathetic nerves non-segmentally. Therefore, sympathetic nerves are likely involved in the proprioception of the spinal column.

770/2523

Tytuł oryginału: Vessels involved in the venous outflow from glandular mucosa of hamster stomach.
Autorzy: Moskalewski Stanisław, Biernacka-Wawrzonek Dorota, Klimkiewicz Justyna, Zdun Rafał
Źródło: Folia Morphol. 2002: 61 (2) s.81-87, il., bibliogr. 18 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

Streszczenie angielskie: Blood from the glandular part of hamster gastric mucosa is drained by collecting venules, running from the subepithelial layer towards lamina muscularis mucosae. To visualise vessels involved in the venous outflow, hamsters were exposed to atropine and subjected to intravital ligation of portal vein, causing strong hyperaemia. Distribution of vessels and their connections were studied a) in translucent, flat preparations of the glandular stomach, b) in thic sections of glandular mucosa cleared in the mineral oil, and c) in semi-thin plastic or parafin sections. Collecting venules were drained by single vessels running parallel to the lamina muscularis mucosae (paramuscular venules), which, in turn, joined submucosal veins through opening in muscularis mucosae. Moreover, some collecting and paramuscular venules discharged into venules belonging to vascular triples composed of two venules and arteriole. The triplets were also parallel to muscularis mucosae. Triplets did not form connections with submucosal veins but passed on the abluminal surface of muscularis mucosae. Thus, some structural elements involved in venous outflow from glandular gastric mucosa differ from those in rats, in which vascular triplets were absent and all collecting venules drained into single paramuscular vessels. Conctraction/relaxation of muscularis mucosae may regulat the amount of blood present in the venous system of the mucosa and the diameter of venules. Rhythmical changes of the latter could cause changes in intramucosal pressure, affecting movement of tissue fluid in the mucosa and thus the functio of gastric cells.

771/2523

Tytuł oryginału: Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain.
Autorzy: Ludkiewicz Beata, Wójcik Sławomir, Spodnik Edyta, Domaradzka-Pytel Beata, Klejbor Ilona, Moryś Janusz
Źródło: Folia Morphol. 2002: 61 (2) s.89-96, il., bibliogr. 42 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

szczury

Streszczenie angielskie: Immunohistochemical study of the cholinergic innervation of the parvalbumin- ad calbindin-containing cells in the hippocampus was conducted on 30 rat brains of various postnatal ages: PO, P4, P7, P14, P21, P30, P60 and P180. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and parvalbumin (PV) or calbindin (CB) were analysed using confocal laser-scanning microscope. Obtained data demonstrate that the pattern of cholinergic innervation of calbindin- and parvalbumin-immunoreactive hippocampal neurones shows some differences. During development as well as in the adult species cholinergic terminals preferentially innervate CB-containing neurones, while cholinergic terminals on PV-containing cells were observed rarely. Cholinergic endings on the CB-ir neurones are localised both on their somata and dendrities, whereas on PV-ir cells they form synaptic contact predominantly with processes. In spite of the unquestionable cholinergic influence particularly on CB-ir cells, the number of cholinergic endings suggests that this input seems not to be crucial for the activity of the studied cell populations.

772/2523

Tytuł oryginału: Preliminary study of external itneratrial muscle fascicles.
Autorzy: Kozłowski Dariusz, Kamiński Rafał, Piwko Grzegorz, Gawrysiak Marcin, Owerczuk Adam, Piszczatowska Grażyna, Budrejko Szymon, Kozłowska Magdalena, Duda Barbara, Grzybiak Marek
Źródło: Folia Morphol. 2002: 61 (2) s.97-101, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The atria are highly complex multidimensional structures composed of a heterogenous branching network of subendocardial muscular bundles. The relief of the inner part of the right atrium includes the crista terminalis as well as multiple pectinate muscles that bridge the thinner atrial free walls and appendages. However, a handful of studies have focused attention on the role of the naturally occurring complexities of the atrial subendocardial muscle structures in the mechanisms of cardiac arrhythmias. In accordance with the facts mentioned above, it was decided to examine the morphology and topography of the external interatrial junctions and related structures in order to define the possible anatomical basis of impulse propagation in focal atrial fibrillation. Research was conducted on material consisting of 15 human hearts of both sexes (female - 6, male - 9) from 18 to 82 years of age. In addition we were concerned, on the basis of the history and electrocardiograph tracings, that none of the patients had shown focal and non-focal type of atrial fibrillation. The classic macroscopic methods of anatomical evaluation were used. The walls of the atria were prepared via a stereoscopic microscope, the pericardium and fatty tissue were eliminated from the surface of the atria, visualising muscle fibres linking both of the atria, and the beginnings and the endpoints of fascicles in the right and left atrium were estimated. The structure, large muscle bundle, was present in all examined hearts. The muscle fascicle was descending from the anterior wall of the right atrium just below the orifice of the superior vena cava. The fascicle...

773/2523

Tytuł oryginału: Evaluation of extralaryngeal diameters in senile individuals.
Autorzy: Zieliński Rafał, Jędrzejewski Kazimierz S.
Źródło: Folia Morphol. 2002: 61 (2) s.103-106, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was a morphometrical macroscopic evaluation of extralaryngeal diameters in elderly people, according to its usefulness in ORL diagnostic and operational methods. Laryngeal preparations together with surrounding structures were taken from cadavers of both sexes, aged 65 and over, about 24 hours after death. Clinically important extralaryngeal diameters were collected using common morphometrical methods. A few body features were also gathered. Computer statistical methods were used in data assessment including basic statistics and linear correlations between diameters, and between diameters and body features. The data presented in the study may be very helpful in diagnostic methods' evaluation. They may also help in: selection of the appropriate operational tool size, choice of the most appropriate operational technique, preoperative preparations and designing and building virtual models for physicians' training.

774/2523

Tytuł oryginału: Morphometric parameters of the human pineal gland in relation to age, body weight and height.
Autorzy: Golan Janusz, Torres Kamil, Staśkiewicz Grzegorz J., Opielak Grzegorz, Maciejewski Ryszard
Źródło: Folia Morphol. 2002: 61 (2) s.111-113, tab., bibliogr. 14 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was to compare the size, weight, volume and density of the pineal gland in several groups divided by age, body weight and height. 80 human pineal glands were included in the study. Obtained data were statistically analysed by means of Statistica by Statsoft to check existing differences. Obtained data show some significant differences between pineal gland morphometry in weight- and height-related groups. However, these differences do not influence pineal volume significantly. Differences between the pineal gland volume in the maximal and in the minimal weight groups may suggest some relationship between the gland's structure and body weight. No age-related changes in the morphometry of the pineal gland were observed.

775/2523

Tytuł oryginału: Distribution of various types of oligodendrocytes and cellular localisation of iron in the frontal cortex of the adult rat.
Autorzy: Wawrzyniak-Gacek Agata
Źródło: Folia Morphol. 2002: 61 (2) s.115-121, il., bibliogr. 36 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Oligodendrocytes, called oligodendroglia, are present in the white and grey substance of the CNS. They constitute a heterogeneous group, in which 3 types of these cells have been distinguished: those of light, medium and dark cytoplasm. They locate themselves at the neurones and blood vessels. The number of light oligodendrocytes decrease with age, while the number of dark oligodendrocytes increase. In histochemical studies the product of the reaction located itself in the body and processes, indicating the participation of these cells in the metabolism of iron.

776/2523

Tytuł oryginału: Prenatal development of the human trachea.
Autorzy: Adamiec Ewa, Dzięciołowska-Baran Edyta, Czerwiński Florian, Miklaszewska Danuta, Teul Iwona
Źródło: Folia Morphol. 2002: 61 (2) s.123-125, il., bibliogr. 8 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The tracheas of 46 human foetuses were examined: 26 male, 20 female with C-R length of 99-255 mm. The analysis of the total length of the trachea was carried out and its transverse dimension was presented on three levels: on the first tracheal cartilage, in the mid-length of the trachea, and at the bifurcation into the main bronchi; also the proportions between these dimensions and the C-R length were analysed. The length of the trachea was measured in a sraight line - from the end of larynx to the bifurcation of the trachea. Comparing tracheas to a tunnel with two openings, it was observed that the transverse dimension, the "laryngeal", is greater than the "bronchial" - in the case of smaller foetuses by 2 mm, but in foetuses with C-R length of over 250 mm by 1 mm. Moreover, no signficant differences between male and female sex were noticed. All the measurements were taken with the use of a slide caliper. The results should be useful in the detection of abnormalities in the structure of the trachea, in problems with respiratory physiology and in endotracheal intubation, endoscopy or tracheostomy.

777/2523

Tytuł oryginału: Die Schallverarbeitung in der Cochlea.
Tytuł polski: Przetwarzanie dźwięku w ślimaku.
Autorzy: Zenner Hans-Peter
Źródło: Otolaryngol. Pol. 2002: 56 (6) s.657-660, il., bibliogr. 30 poz., streszcz.
Sygnatura GBL: 313,466

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie polskie: Na podstawie danych z piśmiennictwa oraz własnych autora badań i doświadczeń w pierwszej części pracy w sposób poglądowy, prosto i przekonująco zostały przedsatwione zjawiska transdukcji i transformacji zachodzące w ślimaku pod wpływem stymulacji dźwiękowej. W drugiej części pracy natomiast opisano specyfikę częstotliwościową ślimaka wyjaśniającą podstawy zrozumiałości mowy i odbioru muzyki. Podstawy teorii fali wędrującej laureata nagrody Nobla Bekesego (którego, jak podkreśla autor, swego czasu wyganano z Niemiec) zostały uzupełnione ostatnimi odkryciami w zakresie czynności komórek słuchowych zewnętrznych jako wzmacniacza bodźców docierających do komórek słuchowych wewnętrznych.

778/2523

Tytuł oryginału: Mycosis fungoides of the larynx: case report and review of the literature.
Tytuł polski: Mycosis fungoides krtani: opis przypadku oraz przegląd piśmiennictwa.
Autorzy: Lippert B. M., Hft S., Teymoortash A., Wiedow O., Rvert J., Werner J. A.
Źródło: Otolaryngol. Pol. 2002: 56 (6) s.661-667, il., tab., bibliogr. 31 poz., streszcz.
Sygnatura GBL: 313,466

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Mycosis fungoides jest rzadką postacią chłoniaka skóry. Uogólnienie procesu chorobowego na okolicę skóry zewnętrznej powoduje, że choroba ta należy do schorzeń o poważnym przebiegu. Objawy choroby umiejscowione w krtani są tylko w 13 przypadkach przedstawione w literaturze dostępnej. W pracy przedstawiono przypadek mycosis fungoides krtani o terminalnym zaawansowaniu schrzenia, dodatkowo zajmujacym zatokę szczękową lewą. Przedstawiono również zestawienie opublikowanych do chwili obecnej przypadków. W ocenie autorów praca przedstawia pierwszy przypadek lokalizacji mycosis fungoides obejmującą krtań oraz zatoki przynosowe. W podsumowaniu zaznaczono, że na podstawie przyadku własnego oraz danych pochodzących z dostępnej literatury wskazane jest w tych przypadkach stosowanie leczenia paliatywnego, aby uzyskać jak najlepszą jakość życia u chorych.

779/2523

Tytuł oryginału: Lipoma of the larynx. Report of 2 cases and review of the literature.
Tytuł polski: Tłuszczak kratni. Opis dwóch przypadków oraz przegląd piśmiennictwa.
Autorzy: Lippert B. M., Eggers S., Schlter E., Rudert H., Werner J. A.
Źródło: Otolaryngol. Pol. 2002: 56 (6) s.669-674, il., bibliogr. 31 poz., streszcz.
Sygnatura GBL: 313,466

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Tłuszczaki jako pierwotne guzy mezenchymalne występują bardzo rzadko w gardle dolnym i krtani, często w badaniu makroskopowym przypominają torbiele retencyjne. W piśmiennictwie opisano 112 przypadków. Przedstawiono 2 przypadki tłuszczaków krtani, które usnięto endoskopowo oraz z dojścia zewnętrznego. Nie zaobserwowano wznów miejscowych.

780/2523

Tytuł oryginału: Investigation of the serological reactivity of the antibodies produced against Citrobacter braakii 037 with human and horse erythrocytes : doctorate thesis
Autorzy: Ebaid Hossam
Opracowanie edytorskie: Gamian Andrzej (supervis.).; Polish Academy of Science Institute of Immunology and Experimental Therapy, Laboratory of Medical Microbiology in Wrocław
Źródło: 2002, [2], 141 k. : il., tab., bibliogr. k. 122-141, sum., maszyn.
Sygnatura GBL: 45/19658

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

781/2523

Tytuł oryginału: Linear IgA bullous dermatosis in a diabetic patient with chronic renal failure.
Autorzy: Serwin Agnieszka Beata, Myśliwiec Hanna, Laudańska Halina, Chodynicka Bożena
Źródło: Int. J. Dermatol. 2002: 41 (11) s.778-780, il., bibliogr. 11 poz.
Sygnatura GBL: 305,645

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: A 60-year-old woman was admitted in October 2000 for disseminated polymorphic papular and vesiculobullous skin lesions with a herpetiform pattern. The eruption occurred 1 month prior to admission and was accompanied by intense itching and burning. A few vesicles in the oral mucosa appeared 6 months after the onset of the skin lesions. The patient's history was notable for noninsulin-dependent diabetes mellitus since the age of 32 years, complicated by arterial hypertension, neuropathy, and nephropathy with subsequent renal failure. The patient started hemodialysis 20 years after the onset of diabetes and was hemodialyzed three times a week for 4 h (index of dialysis adequacy (Kt/v) - 1.3). She had received insulin since the age of 55 years. Furosemide, started in 1998, was withdrawn in October 2000, a few days after admission. Histopathologic examination of the skin lesions revealed subepidermal blisters with an infiltrate of polymorphonuclear cells within the timps of dermal papillae. A diagnosis of linear immunoglobulin A (IgA) bullous dermatosis was confirmed by direct immunofluorescence assay of perilesional skin showing linear IgA (++) and IgG (+) deposits within the basement membrane zone. No antiepidermal basement membrane zone or antiendomysial IgA antibodies were detected in the patient's serum. Abnormal laboratory values were as follows: hemoglobin concentration, 10.6 g/dL; red blood cell count, 3.1 x 10**6 ćL; platelets, 99.0 x 10**3/ćL; glucose concentration, 228.0 mg/dL; creatinine concentration, 5.3 mg/dL; glycosuria and proteinuria...

782/2523

Tytuł oryginału: Formalin-induced pain and ć-opioid receptor density in brain and spinal cord are modulated by A1 and A2a adenosine agonists in mice.
Autorzy: Borghi Valentina, Przewłocka Barbara, Labuz Dominika, Maj Marcin, Ilona Obara, Pavone Flaminia
Źródło: Brain Res. 2002: 956 (2) s.339-348, il., tab., bibliogr. 41 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The effects of adenosine analogues on pain have been shown to depend on the subtype receptor involved as well as on the nociceptive stimuli and on the route of administration. In the first exeperiment of the present study intraperitoneal administration of the A1 receptor agonist N**6-cyclopentyladenosine (CPA) (0.015, 0.03, 0.09, 0.15, 0.21, 0.3 mg/kg) induced dose -dependent analgesia to formalin pain in both phases characterizing the test. The A2a receptor agonist 2-[p]2-(carbonyl-ethyl)-phenyethylamino]-5'-N-ethylcarboxaminoadenosine (CGS21680) (0.025, 0.05, 0.1, 0.15 mg/kg) significantly affected behavioral responses to formalin only during the early phase. In the second experiment the interaction between adenosine and the opioid system was investigated through both behavioral and neurochemical studies. The opioid antagonist naltrexone (0.1 mg/kg) did not affect the antinociception induced by CPA (0.21 mg/kg) and CGS21680 (0.05 mg/kg). Autoradiographic studies showed that formalin administration significantly modified ć-opioid receptor density in the superficial laminae of the spinal cord and in the paracentral thalamic nucleus, contralateral to the side of formalin injection. CPA and CGS21680 counteracted these effects induced by formalin. In conclusion the present study confirms and extends the role of A1 and A2a adenosine receptors in the modulation of inflammatory pain and their interaction with the ć-opioid system, and suggests further invetigation of these purinergic receptors from a therapeutic perspective.

783/2523

Tytuł oryginału: Environmental manipulation differentially alters c-Fos expression in amygdaloid nuclei following aversive conditioning.
Autorzy: Nikolaev Evgeni, Kaczmarek Leszek, Zhu Shun Wei, Winblad Bengt, Mohammed Abdul H.
Źródło: Brain Res. 2002: 957 (1) s.91-98, il., bibliogr. 50 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: This study analyzes the impact of environmental complexity in adult rats on their emotional behavior and c-Fos expression (a transcription factor protein implicated in neuronal plasticity) in various subdivisions of amygdala, as well as selected parts of the thalamus and hypothalamus. The animals were housed for 60 days in either enriched or impoverished conditions and then one group of rats was tested in an open field test, and a second group of rats was treated to footshock-motivated aversive training. Two and 24 h later, the animals from the second group, along with the appropriate controls, were sacrificed and their brains were used for the immunocytochemical analysis of c-Fos levels. We found that this long-term environmental manipulation exerts significant effects on animals' emotionality and this behavioral diference is accompanied by the differential c-Fos activation (at 2 h after the aversive training) in the amygdala, a brain structure believed to subserve emotional reactions. On the other hand, no difference was found in c-Fos expression between both groups of animals in the thalamus and hypothalamus. At 24 h after the training, c-Fos levels were down to the values observed in naive rats that did not differentiate between enriched versus impoverished breeding conditions. These results may help to explain differential emotional aspects of behavior that arise following differential housing conditions of adult animals.

784/2523

Tytuł oryginału: Cross-tolerance between the different ć-opioid receptor agonists endomorphin-1, endomorphin-2 and morphine at the spinal level in the rat.
Autorzy: Labuz D., Przewłocki R[oman], Przewłocka B[arbara]
Źródło: Neurosci. Lett. 2002: 334 (2) s.127-130, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: In the present study we investigated the development of tolerance to an antinociceptive effect after intrathecal administration of endomorphin-1, endomorphin-2 and morphine in tail-flick and paw pressure tests. We also assessed crosstolerance between the antinociceptive effects of the two endogenous ć-opioid receptor agonists - endomorphins and morphine. The tolerance developed on day 3 after i.th. injection of both endomorphins, endomorphin-2 (18, 36 nmol), endomorphin-1 (16 nmol). After morphine (30 nmol) the tolerance developed on day 6. Our study described the crosstolerance between morphine and endomorphin-1, but not endomorphin-2. In comparison with naive rats, morphine had a weaker antinociceptive effect in rats tolerant to endomorphin-1. In contrast, no cross-tolerance was observed after administration of endomorphin-2 in rats tolerant to endomorphin-1. In rats tolerant to endomorphin-2, the antinociceptive effect of morphine and endomorphin-1 was attenuated in both the tests used. Our results suggest that the three lignds of ć-opioid receptors probably act via different subtypes of the ć-opioid receptor.

785/2523

Tytuł oryginału: Trace elements distribution in renal cell carcinoma depending on stage of disease.
Autorzy: Dobrowolski Zygmunt, Drewniak Tomasz, Kwiatek Wojciech, Jakubik Piotr
Źródło: Eur. Urol. 2002: 42 (5) s.475-480, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 312,572

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Objectives: The aim of this study was to identify those trace elements which can be used to distinguish between normal and malignant tissue in renal cell cancer (RCC) kidney and to assess changes in trace elements concentration in tissue with progressing malignant disease. Methods: In case control study, 36 cases of RCC were analyzed by Synchrotron Radiation Induced X-ray Emission (SRIXE) in order to establish the concentration of 19 elements. Patients with RCC were examined to obtain staging of disease after radical nephrectomy, which was performed in each case. Results were compared with 15 control kidney cortex tissue obtained during autopsy in which cause of death was trauma. Results: The most relevant decrease was detected in Cd content: from 81 ń 39.2 ppm in normal control samples to 16.6 ń 22.2 ppm concentration in RCC. We found that the concentrations of Ti, Pb and Rb were also lower in RCC tissue. On the other hand, the RCC tissue was rich in iron and zirconium. With the progress of malignant disease, assessed by TNM (UICC 1997) scale, lower concentration of S and higher concentration of Ca in both RCC and neoplastic kidney cortex can be seen. The same tendency is observed in Zn and Se concentrations. Cadmium shows raising concentration with progress of RCC only in cortex of neoplastic kidney. In all cases it was shown that the relatively high tissue concentration of iron in both investigated tissues is decreasing with the progress of disese. The zirconium has shown raising tissue concentration in advanced disease...

786/2523

Tytuł oryginału: Retroperitoneoscopic nephrectomy in the prone position in children (point of technique).
Autorzy: Urbanowicz Wiesław, Wieczorek Maria, Sulisławski Janusz
Źródło: Eur. Urol. 2002: 42 (5) s.516-519, il., bibliogr. 15 poz.
Sygnatura GBL: 312,572

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

niemowlęta

dzieci 6-12 r.ż.

Streszczenie angielskie: We describe our initial experience with retroperitoneoscopic procedures in children in the prone position. Twelve children (five girls and seven boys; mean age 3.5 years) underwent nephrectomy, nephroureterectomy or heminephrouretectomy for obstruction or reflux within the non-functioning kidney, multicystic dysplastic kidney or upper pole dysplastic moiety. None of the operations were converted to an open procedure. The mean operative time for these procedures was 110 minutes. Blood loss was less than 20 ml.

787/2523

Tytuł oryginału: Immediate hypersensitivity in bullous pemphigoid. A review of our own data and the literature.
Tytuł polski: Nadwrażliwość natychmiastowa w pemfigoidzie pęcherzowym. Przegląd danych własnych i piśmiennictwa.
Autorzy: Bowszyc-Dmochowska Monika, Dmochowski Marian
Źródło: Post. Dermatol. Alergol. 2002: 19 (1) s.7-16, il., bibliogr. 139 poz., streszcz.
Sygnatura GBL: 313,632

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: W pracy przedstawiono zjawiska nadwrażliwości natychmiastowej w pemfigoidzie pęcherzowym (BP). Eozynofile są istotne w patogenezie ludzkiego PB. Pobudzenie tych komórek w BP może prowadzić nie tylko do uszkodzenia tkanki, ale może najprawdopodbniej także być elementem regulacji odpowiedzi immunologicznej w tej chorobie. Pobudzenie komórek Th2 stymuluje produkcję autoprzeciwciał IgG4 i IgE przeciwko białkom półdesmosomalnym, antygenowi BP o masie 180 kDa i antygenowi BP o masie 230 kDa.

788/2523

Tytuł oryginału: Relationship between body composition, sex hormones and leptinemia in hemodialyzed patients with chronic renal failure.
Autorzy: Chudek J., Adamczak M., Kokot F[ranciszek], Karkoszka H., Ignacy W., Klimek D., Więcek A.
Źródło: Clin. Nephrol. 2002: 58 (6) s.431-437, il., tab., bibliogr. [37] poz.
Sygnatura GBL: 312,466

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Female are characterized by significantly higher plasma leptin concentration than males. It seems likely that sex hormones influence leptinemia independently from differences in body composition. The aim of the present study was to analyze the contribution of plasma concentrations of testosterone and estradiol on leptinemia in hemodialyzed patients. Methods: 110 hemodialyzed patient - HD (60 M. 50 F) and 70 healthy subjects (HS) (30 M, 40 F) were enrolled in this study. Plasma leptin, testosterone or estradiol and CRP concentrations and body composition by dual-energy X-ray absorptiometry (DEXA) were assessed. Results: Total body fat was significantly higher in femals than in males (27.5 ń 1.5 p.c. vs. 17.2 ń 1.0 p.c. of body weight in HD and 36.0 ń 1.0 p.c. vs. 18.2 ń 1.4 p.c. in HS, respectively). Plasma leptin concentrations were markedly higher in females than in mals both in HD (27.9 ń 5.4 ng/ml vs. 9.6 ń 1.9 ng/ml) and HS (16.5 ń 1.7 ng/ml vs. 3.1 ń 0.4 ng/ml). A highly significant, strong positive correlation was found between total fat mass (TFM) and leptinemia in all studied groups. No significant univaried correlation between plasma leptin and testosterone or estradiol concentrations respectively was found both in HD and HS. Multiple regerssion analyses showed that the main determinant of leptinemia is TFM (á = 0.623 and 0.798 in HS females and males respectively, and á = 1.058 and 0.797 in HD females and males respectively). Plasma concentration of testosterone (á = -0.139 and á = - 0.075 in male HD and HS respectively)...

789/2523

Tytuł oryginału: Trimetazidine limits the effects of myocardial ischaemia during percutaneous coronary angioplasty.
Autorzy: Poloński Lech, Dec Iwona, Wojnar Rafał, Wilczek Krzysztof
Źródło: Curr. Med. Res. Opin. 2002: 18 (7) s.389-396, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 305,759

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: This open-label, randomised, controlled study is aimed at assessing the effect of pre-treatment with the metabolic agent trimetazidine on the degree of ischaemia during percutaneous transluminal coronary angioplastry (PTCA). Overall 44 patients with one-vessel coronary artery stenosis ( 70 p.c.) in the medial part of the left anterior descending artery were included. One group (n = 22) was pre-treated with oral trimetazidine. The other group (n = 22) was the control. All patients (n = 44) were administered aspirin and conventional treatment. All patients underwent PTCA; stents were implanted in 11 trimetazidine patients and in seven control patients. The mean ST-segment elevation during all balloon inflations was significantly lower in the trimetazidine group than in the control group (-1.66 ń 1.50 mm vs. 3.29 ń 1.59 mm, p = 0.001). Maximal ST-segment elevations and mean ST elevation values during sequential balloon inflations were also significantly lower weith trimetazidine (p = 0.018). The mean amplitude of the T-wave alterations during all ballon inflations was significantly lower with trimetazidine (3.09 ń 2.39 mm vs. 6.83 ń 4.31 mm; p = 0.001). Similary, the maximal amplitude of the T-wave alterations was 4.50 ń 2.90 mm with trimetazidine vs. 9.25 ń 4.97 mm in control patients (p = 0.0005). Angina and rhythm disturbances were more frequent in the control group. Time from balloon inflation to onset of angina was 50 ń 26.2 s with trimetazidine vs. 32 ń 15.0 s, for control group (p = 0.03). The time to pain relief after deflation was 19.3 ń 11.4 s with trimetazidine vs. 28.2 ń 16.8 s (p = 0.001)...

790/2523

Tytuł oryginału: Limb lengthening in automatic mode.
Tytuł polski: Wydłużanie kończyn metodą automatyczną.
Autorzy: Ivanovich Shevtsov Vladimir, Vasilyevich Popkow Arnold
Źródło: Ortop. Traumatol. Rehabil. 2002: 4 (4) s.403-412, il., tab., bibliogr. 28 poz., sum.
Sygnatura GBL: 313,531

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie polskie: Wstęp. W ośrodku naukowym im. Ilizarowa w Rosji wykonano ponad 5000 zabiegów wydłużenia kości kończyn górnych i dolnych, w tym 406 odcinków wydłużonych w systemie automatycznym. Rozciąganie wykonywano całodobowo, w tempie 1 mm na 60 cykli operacyjnych aparatu samorozciągającego. Materiał i metoda. Wydłużanie w trybie automatycznym wykonano u 171 pacjentów w wieku od 5 do 43 lat. Zastosowano techniki jednoodcinkowe i wieloodcinkowe. Długość wydłużenia kończyn mieściła się w przedziale od 3 do 16 cm. Średnia wartość wydłużenia za pomocą metody osteosyntezy jednoodcinkowej wynosiła 6,1 ń 2,0 cm, natomiast za pomocą metod wieloodcinkowych - 7,2 ń 1,5 cm. Mierzono również siły rozciągania rozwinięte w aparacie samorozciągającym. W celu opracowania obiektywnej oceny zalet dystrakcji metodą automatyczną obliczyliśmy współczynnik stabilizacji w aparacie samorozciągającym na jeden centymetr wydłużenia. Wyniki i wnioski. Współczynnik stabilizacji wynosił tylko 5-6 dni na 1 cm, przy użyciu techniki automatycznego rozciągania, natomiast za pomocą ręcznej regulacji wydłużania, aż 22-24 dni na 1 cm. W badaniach biochemicznych metabolizmu kostnego, podczas wydłużenia, wykazano mierzalną aktywność takich enzymów krwi, jak np. fosfotazy zasadowe i kwaśne, jak również duże ilości minerałów w obrębie zachodzącej osteogenezy związanej z rozciąganiem.

791/2523

Tytuł oryginału: TRAIL induces death of human oligodendrocytes isolated from adult brain.
Autorzy: Matysiak Mariola, Jurewicz Anna, Jaskólski Dariusz, Selmaj Krzysztof
Źródło: Brain 2002: 125 (11) s.2469-2480, il., bibliogr. [41] poz.
Sygnatura GBL: 300,909

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been reported to induce apoptosis in various tumour cell lines and, recently, also in normal cells. TRAIL interacts with four receptors: two signalling receptors (TRAIL-R3 and TRAIL-R4). We have shown that both signalling receptors are present on the surface of oligodendrocytes isolated from adult human brain (ahOL), whereas the decoy receptors are expressed at a low level on ahOL. TRAIL induces ahOL apoptosis - as charcterized by Annexin V staining prior to propidium ioddide cell uptake - under conditions of protein synthesis inhibition. However, pretreatment of ahOL with interferon ç (IFNç) evoked susceptibility to TRAIL-induced death, which did not require inhibition of protein synthesis. A blocking experiment with monoclonal antibodies directed gainst TRAIL-R1 and TRAIL-R2 revealed that TRAIL-R1 is mainly involved in TRAIL-induced apoptosis of ahOL. In contrast to ahOL, microglial cells were completely resistant to cell death induced by TRAIL. Microglial cells had high surface expression of the decoy receptor TRAIL-R3, suggesting that resistance of these glial cells to TRAIL-induced death depends onthe presence of the protective effect of TRAIL-R3. Stimulation of microglia with TRAIL increased further expression of TRAIL-R3, but it had no effect on the expression of TRAIL receptors by ahOL. This result may implicate TRAIL as an effector-immune molecule in selective ahOL demise in inflammatory/demyelinating conditions.

792/2523

Tytuł oryginału: Polish consensus: procedure in case of estrogen deficiency in women after breast cancer therapy.
Autorzy: Milewicz A., Szymański W., Rużyłło W., Zgliczyński S[tefan], Reinfus M., Zieliński J., Dębski R., Demissie M., Tworowska U.
Źródło: Gynecol. Endocrinol. 2002: 16 (5) s.385-389, bibliogr. 30 poz.
Sygnatura GBL: 313,072

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Women surviving breast cancer in the postmenopausal period suffer from hormonal alternations with adverse effect on mental status and functioning of a number of organs and systems. Two thirds of these women had menopause before the diagnosis of breast cancer. In the remaining one-third ovarian failure is natural or induced by chemotherapy. Doctors cautiously approach the use of estrogen therapy in this group of patients. Their fears are not unsupported bearing in mind known epidemiological data exist linking breast cancer with the use of hormonal therapy. The purpose of this review is to evaluate current data on hormonal use and breast cancer risk.

793/2523

Tytuł oryginału: L-AP4, a potent agonist of group III metabotropic glutamate receptor, decreases central action of angiotensin II.
Autorzy: Fedosiewicz-Wasiluk Małgorzata, Hoły Zdzisław Z., Wiśniewski Konstanty
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.415-422, il., bibliogr. 32 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The study was designed to investigate the role of the activation of class III metabotropic glutamate receptors (mGluRs) in the behavioral activity of angiotensin II (AngII). The experiments were performed on adult male Wistar rats. Stimulation of group III mGluRs was evoked by icv injection of agonist, L-2-amino-4-phosphonobutyric acid (L-AP4) (5 ćl of 80 mM solution of L-AP4). Fifteen minutes later, the animals were given icv solution containing 1 nmol of AngII. Memory motivated affectively was evaluated by passive avoidance and active avoidance responses (CARs). Moreover, the speculative influence of the treatment on motor activity was tested in open field. We observed that both compounds did not have significant influence on motor activity of rats in open field tesrt. L-AP4 given alone had no influence on acquisition, consolidation and recall of passive avoidance responses. Examination of influence of L-AP4 on the acquisition of CARs, while it did not alter extinction of these responses. AngII, as repeatedly shown before, greatly increased passive avoidance latency, rate of acquisition of CARs and decreased they extinction. Pretreatment of rats with L-AP4 prevented all above behavioral effects of the AngII administration.

794/2523

Tytuł oryginału: Muscimol changes hypoxia-induced imapirment of behavior in rats.
Autorzy: Oksztel Robert, Car Halina, Wiśniewski Konstanty
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.423-431, il., tab., bibliogr. 48 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Muscimol, a seleactive agonist of GABA-A receptors, causes changes in behavioral activity. Hypoxia interferes with the GABAergic system and with the functions of GABA-A receptors. We used muscimol in Wistar rats to extimate its influence on locomotor activity in the open field test as well as on the processes of consolidation and retrieval, evaluated in the test of passive conditioned reflexes. Anxiolytic activity was examined in the elevated "plus" maze in physiological state and after hypoxia-induced amnesia. Following intraperitoneal administration of muscimol (1 mg/kg, ip), the animals showed a decrease in motility, in retrieval of skill reflexes and in a number of entries into open and closed arms in the elevated plus "maze". In animals exposed to hypoxia, we observed reduced motility in the open field, inhibition of retrieval and consolidation of passive conditioned reflexes, shortened time of sojourn in open arms and decreased number of entries into open and closed arms. In the goroup of animals which underwent hypoxia and then received muscimol, we observed no effect of hypoxia on muscimol activity in the open field test, except rearing when muscimol action was significantly reduced. Muscimol improved consolidation but not retrieval in comparison with the hypoxic saline-treated group of rats. In the elevated "plus" maze test, treatment of rats with muscimol after hypoxia significantly prolonged the time spent in open arms and increased the number of entries into open arms, while shortened the time spent in closed arms. In conclusion, muscimol in hypoxia-exposed group of rats exerted beneficial effect on consolidation in passive avoidance situation and exerted anxiolytic activity. Changes in the activity of muscimol under hypoxia may have significant clinical implications.

795/2523

Tytuł oryginału: Hypoxic pulmonary vasoconstriction in isolated blood-perfused rat lung; modulation by thromboxane A2, platelet-activating factor, cysteinyl leukotrienes and endothelin-1.
Autorzy: Chłopicki Stefan, Bartuś Joanna B., Gryglewski Ryszard J.
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.433-441, il., bibliogr. 49 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Recent evidence suggests that hypoxic pulmonary vasoconstriction (HPV) is mediated by hypoxia-induced closure of voltage-gated potassium channels in pulmonary vascular smooth muscle cells. It is also claimed that various vasoconstrictor mediators such as thromboxane A2 (TXA1), platelet activating factor (PAF), cysteinyl leukotrienes (cys-LTs) or endothelin-1 (ET-1) contribute to HPV. Their role, however, has not been unequivocally accepted. On the contrary, it is well known that endothelium-derived nitric oxide negatively modulates HPV. Since NO counteracts action of vasoconstrictor mediators, we tested the hypothesis that modulatory role of TXA2, PAF, cys-LTs and ET-1 in HPV would become apparent in absence of endogenous NO. For that purpose we assessed contribution of these mediators to HPV in the isolated blood-perfused rat lung pretreated with a nonselective NOS inhibitor, L-NAME. HPV, which was greatly augmented by L-NAME (300 ćM) alone, was inhibited neither by a TXA2 synthase inhibitor (Camonagrel, 300 ćM), nor by a PAF receptor antagonist (WEB 2170, 100 ćM), nor by an inhibitor of five-lipoxygenase-activating protein (MK 886, 10 ćM), nor by a non-selective ET-1 receptor antagonist (LU 302872, 30 ćM). In summary, in isolated blood-perfused rat lung, TXA2, PAF, cys-LTs and ET-1 seem not to be involved in HPV, whereas we confirm the dominant role of endogenous NO in blunting HPV.

796/2523

Tytuł oryginału: Mechanisms of delayed preconditioning with A1 adenosine receptor activation in porcine coronary smooth muscle cells.
Autorzy: Neyeem Mohammed A., Mustafa S. Jamal
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.443-453, il., bibliogr. 49 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnie

Streszczenie angielskie: This study examined the hypothesis that the activation of A1 adenosine receptor (A1AR) induces delayed cellular protection (DCP) in porcine coronary smooth muscle cells (PCSMC). The following groups of cultured PCSMC, subjected to simulated ischemia (SI) at 20 h were studied: (a) SI: with ischemia alone; (b) A1AR agonist chloro-N6-cyclopentyl adenosine (CCPA: CCPA (1 ćM) alone; (c) CCPA + PKC inhibitor chelerythrine chloride (CCL): CCPA and 1 ćM CCL; (d) CCPA + iNOS inhibitor S-methylthiourea (SMT): CCPA and 100 nM SMT; (e) CCPA + KATP channel blocker Glibaenclamide (Glb): CCPA adn 50 ćM Glb; (f) CCPA + mitochondrial KATP channel blocker 5-hydroxydecanoate (5-HD): CCPA and 100 ćM of 5-HD; (g) CCPA + A1AR antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX): CCPA and 1ćM DPCPX. The release of LDH into the medium as well as the amount of LDH remaining in the cells was used as a marker of cellular injury and cell viability. Up-regulation of A1AR, î-PKC, iNOS and HSP 72i was detected through Western blot analysis. The cellular resistance (LDH remaining in the cells) acquired by PCSMC due to CCPA (59.42 ń 1.57) was significantly blocked by CCL; 39.30 ń 2.03; SMT: 41.37 ń 1.98; Glb: 47.24 ń 1.31; 5-HD: 47.69 ń 1.40 and DPCPX: 42.92 ń 0.79. CCPA increased the expression of A1AR (1.30 fold), î-PKC (1.20 fold), iNOS (1.50 fold), and HSP 72i (1.70 fold) compared to the controls. CCPA-induced upregulation of A1AR, î-PKC, iNOS, HSP 72i, and the opening of both mitochondrial and sercolemmal KATP channels may possibly participate in signaling cascade. Our study suggests that A1AR activation up-regulates iNOS, HSP 72i via î-PKC signaling pathway to active both mitochondrial and sarcolemmal KATP channels for cellular protection against SI in the acultured PCSMC.

797/2523

Tytuł oryginału: Influence of diethyldithiocarbamate on the activity of ecto-ATPase in lymphocytes of rats: ex vivo studies.
Autorzy: Purzyc Leszek, Całkosiński Ireneusz, Polak-Jonkisz Dorota
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.455-467, il., tab., bibliogr. 42 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Influence of diethyldithiocarbamate (DTC) on the activity of ecto-ATPase (plasma membrane-bound enzyme participating in a cascade of reactions leading to the formation of adenosine - a modulator of inflammation) was examined on the lymphocytes isolated from the spleen of rats with inflammation. DTC was administered at doses of 4 mg/kg and 290 mg/kg using two modes of administration. It has been observed that: a) an inflammation caused an increase in ecto-ATPase activity in both subpopulations of lymphocytes; in the case of B-lymphocytes, the maximum of activity occurred 48 h and in the cases of T-lymphocytes, 72 h after the injection of carrageenin; b) a single injection of DTC at boht doses, 24 h before or 24 h after carrageenin injection caused a decrease in ecto-ATPase activity in B-lymphocytes and its incresase in T-lymphocytes throughout the whole measurement period, which was not observed when DTG was administered only after provocation of inflammation; c) administration of a high dose of DTC togehter with equimolar doses of disulfiram and CS2 led to a decrease in ecto-ATPase activity and 5'-nucleotidase level in B-lymphocytes, which is bound to the former enzyme; d) in in vitro studies, both populations of lymphocytes isolated from the rats treated with a four-fold dose of DTC showed higher resistance of ecto-ATPase to inhibitors of the enzyme and antagonists of type P2 purinoceptors.

798/2523

Tytuł oryginału: Effects of combination of cyclosporine with losartan or enalapril on kidney function in uremic rats.
Autorzy: Azzadin Arsalan, Małyszko Jolanta, Małyszko Jacek S., Tankiewicz Anna, Myśliwiec Michał, Buczko Włodzimierz
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.469-473, tab., bibliogr. 15 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Long-term treatment with cyclosporine in solid organ transplantation has been shown to be associated with the development of hypertension and nephrotoxicity. Angiotensin-converting enzyme inhibitors have well-known nephroprotective properties and may prevent cyclosporine A (CYA)-induced hypertension. Angiotensin receptor I antagonists have similar properties. The purpose of this study was to investigate if losartan or enalapril could be administered with CYA to reduce its nephrotoxic effect in uremic rats. The studies were performed on the following groups of rats: group I - control; group II - control rats + lossartan; group III - control rats + CYA; group IV - uremic rats; group V - uremic rats + losartan; group VI - uremic rats + CYA; group VII - uremic rats + losartan + CYA, group VIII - control rats + enalapril; group IX - control rats + enalapril + CYA; group X - uremic rats + enalapril; group XI - uremic rats + enalapril + CYA. Pretreatment with CYA, losartan or enalapril in uremic rats resulted in a significant increase in urea and creatinine levels and a decrease in hematocrit. The same effect was observed when uremic rats were given CYA + losartan or CYA + enalapril. Pretreatment with losartan was associated with the increase in the level of CYA much higher than with CYA treatment alone. Similarly, pretreatment with enalapril resulted in a significant increase in CYA concentration in both groups of rats given CYA: uremic and non-uremic. Results of our study show that the treatment with cyclosporine and a combination of losartan or enalapril results in an increase in creatinine...

799/2523

Tytuł oryginału: Effect of capsaicin on ion transport in the caecum of rabbits.
Autorzy: Kosik-Bogacka Danuta, Banach Bolesław, Tyrakowski Tomasz
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.475-482, il., bibliogr. 38 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Effect of capsaicin, a stimulator of C-fibres, on ion transport in the caecum of rabbits was studied using electrophysiological methods, designed to evaluate ionic currents occurring in epithelial tissues. The experiments consisted inmeasuring transepthelial electrical potential difference (dPD) of an isolated fragment of ragbbit's caecum, placed ina Ussing apparatus. The ion transport was modified through incubation in Ringer solution, supplemented with amiloride, bumetanide, and capsaicin. Capsaicin was also administred with persistalting pump. The experiments demonstrated that the inhibition of sodium ions transport caused by incubation with amiloride and incubation with capsaicin slowed down mechanical reaction to electrical potential difference. On the other hand, immediately after the other hand, immediately after the administration, the capsaicin effect on C-fibres modified electrophysiological reaction of the caecum to mechanical stimulation. Physiological and pharmacological experiemnts reveal that a component dependent on activation of C-fobres contributes to the reaction of ion transport activation following mechanical stimulation.

800/2523

Tytuł oryginału: Modulation by cationic amphiphilic drugs of serine base-exchange, phospholipase D and intracellular calcium homeostasis in glioma C6 cells.
Autorzy: Bobeszko Marta, Czajkowski Rafał, Wójcik Magdalena, Sabała Paweł, Lei Lingsheng, Nalepa Irena, Barańska Jolanta
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.483-493, il., bibliogr. 37 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We aimed to assess the effect of three drugs belonging to amphiphilic cations, imipramine, amitriptyline and propranolol, on lipid synthesis and intracellular calcium homeostasis in glioma C6 cells. Antidepressants, imipramine and amitriptyline, had a stimulatory effect on [14C]serine incorporation into phosphatidylserine. Similar effect was induced by propranolol, antidysrhythmic drug and an antagonist of á-adrenergic receptor, but not by isoproterenol, a selective agonist of this receptor. Stimulation of serine base-exchange activity by amphiphilic cations occured at concentration as low as 5 - 25 ćM that may be reached during clinical treatment. At much higher concentration (250 ćM), those drugs also stimulated phospholipase D-mediated synthesis of [14C]phosphatidylethanol and blocked phorbol ester-induced, protein kinase C-dependent phospholipase D activity. The latter effect already occurred at low (25 ćM) concentration of drugs. We have also shown that treatment of the cells with amphiphilic cations (1 mM) produced only a weak incrase in the intracellular Ca2+ concentration and did not affect Ca2+ release from the intracellular stores evoked by nucleotide receptor agonists, ATP and ADP. In contrast, this treatment strongly diminished an unspecific leak of Ca2+ from the endoplasmic reticulum caused by thapsigarin and ionomycin. Mianserin, which is not cationic amphiphilic drug, did not affect phosphatidylserine synthesis and phospholipase D activity and produced heterogenous and chaotic Ca2+ responses. Our results suggest that imipramine, amitriptyline and propranolol may modulate lipid synthesis and intracellular calcium signaling independently of theis action on membrane receptors, most probably by modification of the physicochemical properties of cell membranes.

801/2523

Tytuł oryginału: Distribution of allelic variants of functional C3435T polymorphism of drug transporter MDR1 gene in a sample of Polish population.
Autorzy: Jamroziak Krzysztof, Balcerczak Ewa, Młynarski Wojciech, Mirowski Marek, Robak Tadeusz
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.495-500, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: P-glycoprotein (P-gp), the protein product of MDR1 gene, is an important factor regulating the bioavailability of many therapeutics. Recently, the C3435T polymorphism of MDR1 was correlated to altered expression and function of P-gp in normal tissues. In this study, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess C3435T MDR1 polymorphism in 122 healthy individuals of Slavic origin from the population of central Poland (Łódź and surrounding areas). The detected genotype variant frequencies were as follows: CC in 42 p.c., CT in 41 p.c., and TT in 17 p.c. of the tested subjects (C-allele frequency was 0.62). The frequency of the C-allele is similar to Japanese population and significantly higher than in Caucasians from Western Europe. The results of this study give basis for large-scale C3435T MDR1 genotype-phenotype correlation investigations in Polish population that may be useful to individualize therapy of cancer, HIV-1 infection and some other diseases.

802/2523

Tytuł oryginału: Influence of midazolam on pharmacokinetics of verapamil in rabbits.
Autorzy: Orszulak-Michalak Daria, Owczarek Jacek, Wiktorowska-Owczarek Anna K.
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.501-506, tab., bibliogr. 35 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: Calcium channel blockers can get involved in pharmacological interactions when used concomitantly with other drugs. Previous reports indicate a differential influence of various general anesthetics on verapamil pharmacokinetics. A tendency of a faster transfer of verapamil from the central compartment to the tissue compartment was found to be associated with a slower drug return from the tissue at the given verapamil doses during thiopental or propofol anesthesia. Thus, an increased storage of verapamil in tissue compartment and prolongation of the drug action may occur due to those interactions. The aim of the study was to investigate the influence of midazolam on the plasma concentrations and pharmacokinetic parameters of verapamil after intravenous bolus administration in rabbits during 2 h of observation. Verapamil and midazolam were administered intrtavenously at a dose of 0.2 mg/kg. Verapamil levels in plasma were determined by radioanalysis using [3H]verapamil. Levels of verapamil were determined in plasma at 5, 10, 15, 30, 45, 90 and 120 min after the administration, and plasma disappearance was analyzed according to the non-compartmental and 2-compartmental model. After administration of midzaolam together with verapamil, a decrease in verapamil concentration in plasma was seen, with an increased verapamil transfer to the tissue compartment, increased steady state distribution volume and shortened residency time of the drug in the plasma.

803/2523

Tytuł oryginału: Indoor air studies of fungi contamination of social welfare homes in Białystok and the surrounding area during summer and autumn.
Tytuł polski: Badania powietrza domów pomocy społecznej w Białymstoku i okolicy pod względem występowania grzybów w okresie lata i jesieni.
Autorzy: Krajewsak-Kułak Elżbieta, Łukaszuk Cecylia, Oksiejczuk Elżbieta, Gniadek Agnieszka, Macura Anna B., Lewko Jolanta, Niczyporuk Wiaczesław, Cieślak Wojciech, Wojewodko Agata
Źródło: Mikol. Lek. 2002: 9 (2) s.59-66, il., tab., bibliogr. 21 poz., sum.
Sygnatura GBL: 313,181

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie polskie: Wstęp: W okresie ostatnich lat liczba zakażeń grzybiczych u pacjentów hospitalizowanych ulegla podwojeniu. Cel badania: Ocena występowania grzybów drożdzopodobnych i pleśni w powietrzu w domu pomocy społecznej w Białymstoku i okolicy. Materiał i metody: Zanieczyszczenie powietrza (przed budynkiem, w korytarzach, salach chorych, pokojach socjalnych, kuchniach, łazienkach, toaletach) oceniano przy użyciu urządzenia SAS SUPER 100 (Pbi International). SAS stosowany był przez NASA oraz na pokładzie amerykańsko-rosyjskiej stacji orbitalnej MIR. Posiada on międzynarodowy, ustalony standard pobierania próbek powietrza. Florę grzybiczą ze ścian, podłóg, łóżek, rąk personelu i ubrań uzyskiwano przy użyciu aplikatora Count-Tact applicator i płytki Count-Tact (BioMrieux). Wymazy pobierano ze skóry i przestrzeni międzypalcowych rąk i stóp, paznokci i jamy ustnej. Grzyby hodowano na podłożu Sabourada, a ich identyfikację przeprowadzano przy użyciu standardowych procedur. Wyniki: Badania mikrobiologiczne powietrza oraz ścian wykazały istotne różnice w występowaniu flory grzybiczej w zależności od miejsca izolacji (korytarze, sale chorych, pokoje socjalne, kuchnie, łazienki, toalety itd.) i pory roku (lato, jesień). Znamienny wzrost izolacji grzybów z powietrza i ścian w domach pomocy społecznej stwierdzono jesienią. Podobny wzrost izolacji grzybów wykazano u mieszkanców jesienią. Grzyby drożdżopodobne izolowane od mieszkańców domów pomocy społecznej były wrażliwe na leki ...

Streszczenie angielskie: Introduktion: Durng the recent years the number of fungal infections in hospital patients has doubled. Aim of study: The contamination of the indoor environment with yeast-like fungi and moduls in social welfare homes in Białystok and the surrounding area was evaluated. Material and methods: The air pollution (in front of the building and in the corridors, patient rooms, study rooms, reaction rooms, kitchens, bathrooms, toilets, ventilation systems) was determined using SAS SUPER 100 (Pbi International). The SAS (surface Air System) was used by NASA and in the joint American-Russian space program on board the MIR orbiting station for portable air sampling. The microbial flora from walls, floors, beds, hands of medical stuft and equipment was assessed using the Count-Tact applicator and the plate Count-Tact (BioMrieux). Swabs were taken from the skin of the interdigital spaces of feet and hands, nails and the oral cavity. The fungi from the swabs were cultured on Sabouraud medium. Fungi were identified using standard microbial procedures. Results: Microbiological tests of air and walls revealed significant differences in mycological flora depending on the place of isolation (corridor, rooms, reading room, nurse, room, itchen, dining room, bathroom ect.) and season (summer, autumn). A significant increase in the fungi isolated from the air and walls in the social welfare homes was found, during autumn. An increase in the fungi isolated from residents was found in ...

804/2523

Tytuł oryginału: Enzymatic biotypes of the yeast-like fungi strains and their susceptibilities to antimycotics isolated from ontocenosis of the urogenital system.
Tytuł polski: Biotypy enzymatyczne a lekowrażliwość szczepów grzybów drożdżopodobnych izolowanych z ontocenozy narządów moczowo-płciowych.
Autorzy: Krajewska-Kułak Elżbieta, Łukaszuk Cecylia, Niczyporuk Wiaczesław, Trybuła Jadwiga, Szczurzewski Maciej
Źródło: Mikol. Lek. 2002: 9 (2) s.67-74, il., tab., bibliogr. 21 poz., sum.
Sygnatura GBL: 313,181

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Cel: Określenie biotypów enzymatycznych szczepów Candida albicans wyhodowanych z ontocenozy narządów moczowo-płciowych oraz wykazanie ewentualnej zależności pomiedzy biotypem a lekowrażliwością. Materiał i metody: Badaniem objęto szczepy Candida albicans izolowane z materiału pobranego od pacjentów z kandydoz - 132 szczepów wyhodowanych z ontocenozy cewki moczowej oraz 160 szczepów z ontocenozy pochwy. Aktywnlość enzymatyczną oceniano przy użyciu testu API ZYM firmy bioMrieux. Lekowrażliwość oznaczano za pomocą testu FUNGITEST (Bio-Rad). Biotypowanie szczepów przeprowadzono według klasyfikacji Williamsona oraz Kurnatowskiej i Kurnatowskiego. Wyniki: Szczepy izolowane z ontocenozy cewki moczowej cechowała najwyższa aktywność arylamidazy leucynowej, arylamidazy, N-acetylo-á-glukozy-loamidazy, zaś izolowane z pochwy - arylamidazy leucynowej. esterazy oraz lipazy esterazowej. Szczepy Candida albicans hodowane z cewki w największym odsetku wykazywały cechu biotypu A (59,1 proc.), z pochwy - biotypu F (35 proc.). Izolaty z cewki moczowej były najbardziej wrażliwe na 5-fluorocytozynę, a oporne głównie na itrakonazol. Największą oporność na badane leki przeciwgrzybicze wykazywały szczepy o cechach biotypów I i G. Szczepy z pochwy cechowała największa wrażliwość na amfoterycynę B, a oporność głównie na ketokanazol i mikonazol. Największą oporność na badane leki przeciwgrzybicze miały szczepy o cechach biotypów E i F. Wnioski: Najwięcej izolatów Candida albicans wyhodowanych ...

Streszczenie angielskie: Aim: Determination of the enzymatic biotypes of Candida albicans isolated from ontocenosis of the urogenital system and assessment of possible correlations between biotypes and their susceptibilities. Material and methods: 132 Candida albicans strains isolated from patients with candidiasis of the urethra and 160 from the vagina were evaluated. The enzymatic activity of the Candida albicans strains was determined by using the API-ZYM test Mrieux and their susceptibility using the FUNGI-TEST Bio-Rad. Biotyping of all strains was done according to Williamson's, and Kurnatowska and Kurnatowski's classification. Results: The isolates from the urethra had the highest enzymatic activity of leucine and N-acetyl-á-glucosaminidase from the vagina - leucine arylamidase, esterase and esterase lipase. The enzymatic biotypes of Candida albicans from the urethra had the highest pecentage of biotype A 59.1 p.c. and from vagina biotype F - 35 p.c. The isolates from the urethra had the highest susceptibility to 5-fluorocytosine, the lowest to itraconazole. Strains of biotype I and G had the highest resistance to the antimyocotocs tested. The isolates from the vagina were sensitive to amphotericin B but less sensitive to ketoconazole and miconazole. Biotype E and F had the highest resistance to the drugs tested. In conclusions: Most of the isolayes of candida albicans from the urethra and vagina bekonged to biotypes of Williamson's classification. The isolates from the urethra ...

805/2523

Tytuł oryginału: Clinical characteristics of hereditary ovarian cancer (HOC) in Poland.
Autorzy: Menkiszak Janusz, Górski Bohdan, Jakubowska Anna, Gronwald Jacek, Huzarski Tomasz, Byrski Tomasz, Foszczyńska-Kłoda Małgorzata, Fliciński Jacek, Brzosko Marek, Rzepka-Górska Izabella, Lubiński Jan
Źródło: Ginekol. Pol. 2002: 73 (9) s.733-739, tab., bibliogr. 24 poz., streszcz.
Sygnatura GBL: 301,184

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: W pracy przedstawiono charakterystykę kliniczna dziedzicznego raka jajnika (DRJ) w populacji polskiej. Oceniano wiek zachorowania, stopnie zaawansowania klinicznego (FIGO), stopnie morfologicznej złośliwości (G) oraz częstość występowania raka typu surowiczego. Analizie poddano 142 DRJ z 82 rodzin, które następnie sklasyfikowano na badane grupy i podgrupy na podstawie danych rodowodowo-klinicznych i wyników badan molekularno-genetycznych na obecność mutacji konstytucyjnych genu BRCA1. Zebrane dane poddano analizie statystycznej. Stwierdzono, że cechy kliniczne DRJ w Polsce oraz innych krajach są podobne, a poszerzenie kryteriów rodowodowo-klinicznych o testy DNA umożliwia wykrywanie podgrup DRJ o odmiennych cechach klinicznych.

Streszczenie angielskie: Background: Hereditary ovarian cancer (HOC), as any genetic disease, may display clinical characteristics that depends on population. Aim of the study: The aim of the study was to described clinical characteristics of HOC in Polish population basing on analysis of the following features: age at onset, clinical staging, morphological grading and prevalence of serous adenocarcinoma. Material and method: The cases were selected basing on analysis of pedigree/clinical features and molecular studies of founder mutations of BRCA1 gene in Poland. results: The patients; age at diagnosis was ca 49-52 and was similar in all groups. The exception was the subgroup without mutations in group II (breast and ovarian cancers found in families) with mean age at diagnosis ca 46 years (n = 9). In patients with HOC without mutation of BRCA1 gene, lower FIGO stage and lower morphological grade were detected more frequently. The majority of HOC showed histopathological pattern of serous adenocarcinoma. Conclusions: 1. Clinnical features of HOC in Poland and in other countries are similar. 2. Introduction of DNA test to the clinical and pedigree diagnostic criteria allows detection of subgroups of HOC with different clinical features.

806/2523

Tytuł oryginału: Central nervous system activity of new pyrimidine-8-on[2,1-f]theophylline-9-alkylcarboxylic acids derivatives.
Autorzy: Fhid Omeran, Pawłowski Maciej, Filipek Barbara, Horodyńska Renata, Maciąg Dorota
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.245-254, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The appropriate esters 1-3 were synthesized by the alkylation of unsubstituted pyrimidin-8-on[2,1-f]theophylline I with ethyl-chloroacetate, ethyl-acrylate and ethyl-4-bromobutyrate. The acids 4-6 were obtained by hydrolysis of the esters 1-3, and transformed into Na salts 4a-6a. Amidation of the ester 1 with 25 p.c. ammonia and hydroxylamine led to amide 7 and N-hydroxyamide 8. The bromination of 1 gave 7-bromo intermediate 9 transformed to the 7-amino derivatives via nucleophilic substitution with phenylpiperazine for compound 10 and morpholine for compound 11. In the reaction of 9, 10 and 11 with sodium alcoholate, the corresponding Na salts 12a-14a were obtained. The pharmacological properties of the compounds were tested in CNS activity screening. It was found that all the investigated compounds produced significant sedative effects in behavioral tests except weak activity of pyrimido[2,1-f]theophyline-9-sodium acetate 4a. The potent activity of pyrimido[2,1-f]theophylline-9-sodium butyrate 6a, pyrimido-[2,1-f]theophylline-9-acetamide-7, 7-N-phenylpiperazine-pyrimid[2,1-f]theophylline-9-sodium acetate 13a, and 7-N-morpholine-pyrimido[2,1-f]theophylline-9-sodium acetate 14a was observed. They inhibited spontaneous locomotor activity, potentiated sedation and prolonged duration of sleeping time after thiopental sodium administration. All compounds had no effects in the test of analgesia and they showed weak anti-convulsant properties. The results ofthis investigation may hypnotic, sedative and/or tranquillizing properties of the tested compounds.

807/2523

Tytuł oryginału: Effect of nimesulide on the indomethacin- and ibuprofen-induced ulcer in rat gastric tissue.
Autorzy: Sleyman Halis, Altinkaynak Konca, Gcer Fatma, Maras Ahmed, Akcay Fatih, Onuk M. Derya, Gepdiremen Akcahan
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.255-259, tab., bibliogr. 16 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: In this study, we examined the effect of nimesulide and ranitidine on the indomethacin- and ibuprofen-induced ulcer. In addition, we investigated whether there was a direct chemical interaction of nimesulide and ranitidine with indomethacin, and also whether nimesulide and rantidine neutralized the gastric acid in vitro. Our studies showed that nimesulide (100, 300, 500 ng/kg) prevented the indomethacin-induced gastric ulcers completely. Ranitidine at a dose of 150 mg/kg reduced the mean ulcer area to 2.5 ń 0.76 mmý in the indomethacin-treated and to 2.0 ń 0.81 mmýin ibuprofen-treated rats. The mean ulcer areas in rats given only indomethacin and only ibuprofen were 20.3 ń 1.58 mmý and 9.5 ń 1.43 mmý, respectively. There were no chemical reactions between nimesulide and indomethacin or ranitidine and indomethacin in vitro. Nimesulide and ranitidine did not neutralize the gastric acid in vitro.

808/2523

Tytuł oryginału: Comparison of motor reactivity of the colonic muscularis mucosae isolated from human, guinea pig and rat in vitro.
Autorzy: Kamikawa Yuichiro, Shibukawa Asako, Uchida Kohsuke, Sakuma Atsushi, Kubota Kei-ichi, Ohno Yasuo
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.261-266, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We have compared the reactivity to spasmogens of longitudinal muscularis mucoase isolated from the human, guinea pig and rat colon in vitro. The muscularis mucosae isolated from the human distal colon responded with a sustained contractions to carbachol (10 nM - 30 ćM), in a concentration-dependent manner, and the maximum contraction was comparable to that with potassium concentration (100 mM). Among several spasmogens, neurokinin A was the most potent with the following order of potency: carbachol, prostaglandin F2ŕ an dacetylcholine. Histamine, 5-hydroxytryptamine and bradykinin did not produce a recognizable contraction of this tissue. The muscularis mucosae isolated from the guinea pig distal colon demonstrated a concentration-dependent contraction in response to neurokinin A, carbachol, histamine and acetylcholine, but not to prostaglandin F2ŕ or 5-hydroxytryptamine, and the maximum contraction was obtained with histamine. The muscularis mucoase from the rat distal colon was very sensitive to neurokinin A and bradykinin, less to carbachol and acetylcholine, and not at all sensitive to histamine, 5-hydroxytryptamine and prostaglandin F2ŕ. It is concluded that the colonic muscularis mucoase respond to pharmacological agents in a species-different manner.

809/2523

Tytuł oryginału: Effect of capsaicin and dimethyl sulfoxide on ion transport in the selected experimental models.
Autorzy: Kosik-Bogacka Danuta I., Banach Bolesław, Tyrakowski Tomasz, Wojciechowska Iwona
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.267-274, il., tab., bibliogr. 46 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Żaby

in vitro

Streszczenie angielskie: The aim of the present work was to determine the changes in ion transport in the selected epithelium-lined organs under influence of mechanical stimuli, and also to assess similarities and differences in reactions to capsaicin and dimethyl sulfoxide (DMSO) between trachea and caecum of rabbit and the skin of frog in this experimental setup. The experiments were conducted on rabbit trachea and caecum, and the skin of frog, Rana esculenta L. The experiments consisted in measuring transepithelial electrical potential (PD in mV) with Ussing apparatus, modified to enable testing of the effects of mechanical stimulation of organs and defined pharmacological treatments. It was demonstrated that the addition of DMSO to the stimulating fluid decreased reversible hyperpolarization (dPD) after mechanical stimulation by at least 50 p.c. in all studied groups. On the other hand, action of capsaicin was dependent on the organ studied as well as on experimental conditions (e.g. type of incubation). Capsaicin decreased PD and reaction to mechanical stimulation in trachea incubated in Ringer solution supplemented with amiloride. On the other hand, it did not influence electrophysiological parameters of the trachea following its incubation with bumetanide. Capsaicin did not change electrical potential or reactivity of rabbit caecum incubated with both amiloride and bumetanide. The administration of capsaicin on frog skin incubated with bumetanide casued inhibition of the reaction to mechanical stimulation, whereas during incubation with amiloride no changes were recorded in PD and dPD of the skin. The present study demonstrated that capsaicin and DMSO could modify processes of ion transport dependent on mechanical stimulation.

810/2523

Tytuł oryginału: Effects of some drugs on rat erythrocyte 6-phosphogluconate dehydrogenase: an in vitro and in vivo study.
Autorzy: Ciftci Mehmet, Beydemir Skr, Yilmaz Hayrullah, Bakan Ebubekir
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.275-280, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The in vitro and in vivo effects of some drugs on rat erythrocytes 6-phosphogluconate dehydrogenase were investigated in this study. Rat erythrocyte 6-phosphogluconate dehydrogenase was partially purified with ammonium sulfate precipitation. The enzyme activity was determined by Beutler's method. Some drugs such as ampicilin, amikacin sulfate, and netilmicin sulfate inhibited the enzyme activity in in vitro conditions, while metamizole activated it. The I50 values of the inhibiting drugs were 66.2, 5.836, and 0.963 mM, respectively. For the drugs having low I50 values (drug concentrations which produce 50 p.c. inhibition) (amikacin sulfate and netilmicin sulfate), in vivo studies were performed in rats (Sprague-Dawley). Amikacin sulfate at 64 mg/kg inhibited the enzyme activity significantly (p 0.05) 2 h after dosing. Netilmicin sulfate at 6.4 mg/kg also inhibited the enzyme significantly (p 0.05) 4 h after dosing. Amikacin sulfate and netilmicin sulfate inhibited rat erythrocyte 6-phospogluconate dehydrogenase both in vivo and in vitro. The enzyme was inhibited in vitro by ampicillin and activated in vitro by metamizole.

811/2523

Tytuł oryginału: Homocysteine-induced augumentation of spontaneous contraction of isolated guinea pig myometrium.
Autorzy: Ayar Ahmet, Tug Niyazi, Celik Husnu, Ozcan Mete, Ozcelik Oguz
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.281-284, il., bibliogr. 18 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Recent studies have suggested association between the elevated homocysteine levels and pregnancy complications. The aim of this study was to investigate the effects of homocysteine on spontaneious contractions of myometrium isolated from pregnant guinea pig. Full-thickness myometrial strips were obtained from late-pregnant guinea pigs following decapitation, and suspended in an organ bath which was filled with physiological saline solution (pH 7.4), maintained at 37řC and continuosly bubbled with 95 p.c. O2 and 5 p.c. CO2. After the observation of spontaneous contarctions under one gram of resting tension, homocysteine (0.2, 0.5 and 1 mM) was added to the organ bath and effects off this agent on frequency and amplitude of contractions were evaluated in 20 min periods. One-way analysis of variance was used for statistical analysis of the data. Homocysteine caused increase in both frequency and amplitude of spontaneous contractions in a dose-dependent manner. The results from this in vitro study indicate that cardiovasular risk factor homocysteine has contracting pharmcological effects on guinea pig myometrium.

812/2523

Tytuł oryginału: Prescribing pattern of anti-seizure medications (ASMs): an evaluation of extent of xanthine co-medication.
Autorzy: Manjula Devi, David Joy, Kulkarni Chanda
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.285-291, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: This study was conducted to evaluate the prescribing pattern of anti-seizure medications (ASMs) at a tertiary care hospital. The extent and pattern of concurrently used medications for co-exiting illnesses was also studied. Attention was focussed in particular on co-existence of bronchial asthma with epilepsy and co-medication of ASMs with xanthines. The study was carried out at the Central Pharmaacy and at the Medical Records Department. Data analysis at the central pharmacy showed 3.98 p.c. prescriptions for ASMs, with maximum number for males. More drugs were prescribed during the second decade of life and there were 2.17 drugs per prescription. The data for pattern and extent of use of ASMs along with polutherapy and concurrently used medications revealed the highest number of prescriptions for phenytoin, maximum number with single ASM, and phenytoin with phenobarbitone as most frequently prescribed combination. Co-administartion of ASMs with respiratory medications was found in 2.47 p.c., with 38.8 p.c. prescriptions having xanthines prescribed in them. Xanthines, the well known CNS stimulants, a property attributed to their adenosine receptor antagonistic activity, are considered potential seizurogenic agents. The results of the present preliminary survey show an indirect evidence for co-existence of epilepsy with asthma along with the extent of co-medication of ASMs with xanthines. Results indicate the need for further studies to evaluate the consequences of co-medication of ASMs with xanthines.

813/2523

Tytuł oryginału: Analytical studies on two amino acid derivatives of anticonvulsant activity.
Autorzy: Pirianowicz-Chaber Elżbieta, Paruszewski Ryszard
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.239-241, il., tab., bibliogr. 5 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Qualitative and quantitative methods of analysis of two new amino acid derivatives, potential anticolvulsants of low neurotoxicity: N-acetyl-ç-aminobutyric acid benzylamide (1) and N-methyl-ç-aminobutyric acid benzylamide (2) were developed. Qualitative analysis includes characteristic colour reactions, chromatographic (TLC) investigations, IR and UV spectra interpretation and quantitative analysis involves spectrophotometric, chromatographic (HPLC), acidimetric and argentometric methods.

814/2523

Tytuł oryginału: Stability of cefepime dihydrochloride monohydrate in solid state.
Autorzy: Musiał Wojciech, Zając Marianna
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.243-246, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The pseudo first-order rate constants and thermodynamic parameters for the decomposition of cefepime in solid state in absence of hymidity and at a relative humidity of 76.4 p.c. were calculated. The effect of humidity on the stability of cefepime dihydrochloride monohydrate in the humidity range 25.0 p.c. - 76.4 p.c at 358 K is described by the equation ln ki + (0.031 ń 0.0043) ú RH p.c. - 10.08 ń 0.22.

815/2523

Tytuł oryginału: Studies on the influence of auxiliary substances on the physico-chemical characteristics of ophthalmic drugs. P. 1: Studies on the influence of auxiliary substances on the critical micellar concentration of surfactants solutions in eye drops containing sulphacetamidum natricum.
Autorzy: Pluta Janusz, Meler Jan
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.247-252, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Interactions between Tweens, surfactants used in eye drops and the remaining compounds of the preparations were investigated. The interactions were assessed by means of the surface tension measurement method. Obtained results demonstrate that the substances used in the facture of the preparation affect the critical miclear concentrations of Tweens. Only hydroxyethylcellulose was found to exert a stabilizing effect on the critical micellar concentration of these substances. The amount of used surfactant should be taken into consideration in the manufacture of eye preparations.

816/2523

Tytuł oryginału: Effect of various vehicles on diclofenac sodium and indomethacin pharmaceutical availability.
Autorzy: Stożkowska Wiesława
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.253-260, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The aim of the study was to determine the pharmaceutical availability of various ointment systems containing antirheumatic substances and to estabilish an optimal system for cutaneous application. Topical application permits elimination or at least reduction of side effects connected with oral administration. Three systems were evaluated: emulsion W/O (ointment), emulsion O/W (cream) and gel, all of them containing diclofenac sodium or indomethacin. The investigated systems are characterized by proper rheological parameters and long physiochemical stability. Studies on diclofenac and indomethacin pharmaceutical availability show a good release of the substances from cream and hydrogel bases, but a very poor release from the ointment base.

817/2523

Tytuł oryginału: Investigations of the thymomimetic activity of a selected phytopharmaceutical preparation - reumaherb tablets by flow cytometry.
Autorzy: Prosińska Joanna, Sawicka Teresa, Drozd Janina
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.265-273, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 301,378

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The aim of the present study was to investigate the influence of Reumaherb tablets, a plant preparation, on th ematuration of mice thymocytes in the presence of glycocorticosteroid by the method of cytotoxicity test performed in vitro in two versions differing in methodology. The cell source for incubation was thymus of female Balb/c mice, four weeks old. Reumaherb tablets were added to the culture of thymocytes diluted by a factor of 10-400. The searched thymomimetic activity of the investigated Reumaherb preparation was found in both versions of the test, this being demonstrated by a 17 p.c. increase in the number of surviving cells in the presence of glycocorticosteroid added to the culture. This effect strongly depends on the applied doses. By a microscopic evaluation of the results a maximum stimulation was found at a 200-fold dilution of the examined preparation, while in the cytometric analysis a similar maximum effect was observed at a 20-fold dilution.

818/2523

Tytuł oryginału: Flavonoids from some species of genus Scopolia Jacq.
Autorzy: Nowak Sławomira, Wolbiś Maria
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.275-280, tab., bibliogr. 23 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Kaempferol 3-O-(2-glucosyl)-galactoside-7-O-glucoside was isolated from the leaves of Scopolia carniolica Jacq. and S. sinensis Hemsl. From the latter taxon as well as kaempferol 3-O-galactoside and 3-O-(2-glucosyl)-galactoside, kaempferol and quercetin 3-O-robinobiosides and quercetin 3-O-sophoroside have been obtained. Moreover, from the leaves of S.lurida Dun. kaempferol and quercetin 3-O-glucosides and 3-O-rutinosides were isolated. The structures of compounds have been determined by means of chemical and spectral methods (UV, LSI MS, 1H and 13C NMR, 1H - 1H COSY NMR).

819/2523

Tytuł oryginału: Synthesis and some pharmacological properties of 3-(4-phenyl-5-oxo-1,2,4-triazolin-1-ylmethyl)-1,2,4-triazolin-5-thione derivatives.
Autorzy: Dobosz Maria, Struga Marta, Chodkowska Anna, Jagiełło-Wójtowicz Ewa, Stępniak Krystyna, Kozioł Anna E.
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.281-290, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: In the reaction of (4-phenyl- or 3,4-diphenyl-5-oxo-1,2,4-triazolin-1-ylmethyl)-carbohydrazide (IIa, IIb) with isothiocyanates the thiosemicarbazide derivatives [IIIa, b - IXa, b] were obtained. Cyclization of those compounds in the presence of 2 p.c. or 10 p.c. NaOH led to formation of derivatives with the 1,2,4-triazolin-5-thione system [Xa, b - XVIa, b]. Molecular structure proposed for this group of compounds was confirmed by X-ray structure analysis of Xa and XIVa. Compounds Xa, XIIa and XIVa were investigated pharmacologically for their central properties in mice. It was shown that only compound XIIa produces antinociceptive effects in mice.

820/2523

Tytuł oryginału: Synthesis, structural characterization and antimicrobial studies of hydrazone derivatives of 3-hydroxyimino-5-methyl-2-hexanone.
Autorzy: Donde Kamini J., Patil Vishwanath R., Utekar Shilpa S., Malve Sheela P.
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.291-293, il., tab., bibliogr. 7 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The derivatives of 3-hydroxyimino-5-methyl-2-hexanone oxime have been obtained in good yield by its reactions either with hydrazine hydrate or phenyl hydrazine, respectively. IR and 1H NMR spectral data of these compounds have been discussed. All the newly synthesised compounds have been tested for their biological activity against S. auerus, S. typhi, C. albicans, A. niger, S. cerevisiae and M. tuberculosis H37RV.

821/2523

Tytuł oryginału: Quantitative structure-activity relationships study of a series of imidazole derivatives as potential new antifungal drugs.
Autorzy: Wiktorowicz Włodzimierz, Markuszewski Michał, Krysiński Jerzy, Kaliszan Roman
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.295-306, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The Quantitative Structure-Activity Relationships (QSAR) has been developed to relate antifungal activity against Candida albicans and Rhodotorula glutinis of new imidazole derivatives with their physico-chemical and structural properties. For 265 midazole derivatives the most significant statistically equations has been obtained with correlation coefficients R = 0.800 and R = 0.820 in case of activity against C.a. and Rh.g., respectively. The overall antifungal activity has been described by means of size and bulkiness releated parameters as well as polar and lipophilic interactions. The significance of lipophilicity in terms of n-octanol/water partition coefficient, ClogP, on antifungal potency against both fungi has been investigated. QSAR equations for different classes of antifungal activity have been obtained. With a very high probability level (92 p.c. and 96 p.c.) the weak or wery weak antifungal potency aganst C.a. can be determined and thus the number of required experiments can be reduced.

822/2523

Tytuł oryginału: Effect of chronic hyperglycemia and vanadate treatment on erythrocyte Na/K-ATpase and Mg-ATpase in streptozotocin diabetic rats.
Autorzy: Totan Alexandra Ripszky, Greabu Maria
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.307-311, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: We have studied Na/K-ATPase and Mg-ATPase activities in red blood cells of diabetic rats treated in vivo with sodium vanadate. To our knowledge the effect of in vivo vanadate treatment on these two enzymes has not been studied. Red blood cell Na/K-ATPase plays a central role in the regulation of intra- and extracellular cation homeostasis. Alteration of this transport enzymes is thought to be linked to several complications of diabetes mellitus: hypertension, nephropathy, peripherical neuropathy and microangiopathy. An Mg2+ - dependent ATPase activity located in the erythrocyte membrane appears to be responsible for controlling the smoothing of echinocytic erythrocytes to discocytes and stomatocytes. Our results show that in alloxan diabetes activities of both ATPases are reduced (especially the activity of Na/K-ATPase). Vanadate treatment of normal animals reduced the activities of both enzymes: with 33.08 p.c. for Na/K-ATPase and 22.76 p.c. for Mg-ATPase. Vanadate treatment of diabetic animals did not affect significantly the inhibition process for Na/K-ATPase. For Mg-ATPase we have obtained a significant cumulative inhibition. These results stand out the different functions and physiologic control mechanism of these two ionic pump in red blood cells.

823/2523

Tytuł oryginału: Synthesis of a new antischistosomally active and toxicologically tolerant C-12 monothione surrogate of the universal antihelmintic praziquantel.
Autorzy: Abo-Ghalia M. H., Soliman A. M.
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.313-320, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: A new C-12 monothione mimic (III) of the universal antihelmintic Praziquantel (I) namely, 2-cyclohexylthiocarbonyl(1,2,3,6,7,11b)-hexahydro-4 -pyrazino[2-1a] isoquinoline-4-one was chemically synthesized and structurally elucidated (Elemental analysis, EI-Mass, 13C-NMR and IR spectroscopy). Antischistosomal potency in the order of ~76 p.c. comparable to that for our newly reported C-12 and C-4 dithion mimic II (~70 p.c.) and Praziquantel. Praziquantel (100 p.c., mice infected with S. mansoni cercariae), was realized. Toxicological evaluation (mice liver and kidney functions) and biochemical parameters (cholesterol, triglycerides, albumin, total serum proteins and amino acid profile of liver protein homogenate) were also assayed. Comparable to the parent drug, general insignificant toxicological differences could be attributed for III. Interestingly, III exhibited intermediate biological figures between I and II. An order of II III I with respect to the antischistosomal potency and II III I, for the other tested biochemical parameters was observed. A consideration of obtained results could indicate that, structurally, an intact glycine amide segment of the pyrazine moiety, as it is the case in both I and III, and not in II (glycine thioamide) seemed now more crucial for exhibiting an optimum antihelmintic potency as well as a more tolerant toxicity characteristics. Additionally, the obtained comparable amino acid profile of mice liver protein homogenate after the treatment by III, could suggest ...

824/2523

Tytuł oryginału: Lidocaine elimination in patients with liver cirrhosis.
Autorzy: Wójcicki Jerzy, Kozłowski Kosma, Droździk Marek, Wójcicki Maciej
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.321-324, tab., bibliogr. 15 poz.
Sygnatura GBL: 301,378

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: The aim of the study wa to evaluate lidocaine elimination in patients with liver cirrhosis. The study was carried out in 30 cirrhotic patients classified according to the Child-Pugh's score to subgroups A (n=11), B (n=12) and C (n=7), and 14 helalthy volunteers. Lidocaine was administered intravenously, at a dose of 1 mg/kg, and blood samples for lidocaine and monoethylglycinexylidide (MEGX) assays were collected for up to 6 h. Decreased elimination half-live for lidocaine as well as reduced formation rate of MEGX was found in cirrhotic patients. Lidocaine metabolising capacity of the liver was irrespective of etiology of cirrhosis. It was also found that evaluation of elimination half-life of lidocaine is more closely related to the Child-Pugh's staging of liver dysfunction than 15-minute MEGX concentration.

825/2523

Tytuł oryginału: Quantitative determination of coumarins, flavonoids and chlorogenic acid in the leaves and underground parts of some species of genus Scopolia Jacq.
Autorzy: Nowak Sławomira, Dzido Tadeusz H., Soczewiński Edward, Wolbiś Maria
Źródło: Acta Pol. Pharm. 2002: 59 (4) s.259-263, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 301,378

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The quantitative determination of coumarins, flavonoids and chlorogenic acid in the leaves and underground parts of Scopolia carniolica Jacq., S. lurida Dun. and S. sinensis Hemsl. using the RP-HLP method has been described.

826/2523

Tytuł oryginału: Pharmacological correction of endothelial dysfunction : The 10th Symposium of Jagiellonian Medical Research Centre : final programme
Opracowanie edytorskie: Gryglewski Ryszard (przedm.).
Jagiellonian Medical Research Centre in Cracow
Źródło: - Kraków, JMRC 2002, 92 s. : il., 21 cm. - 10 Sympozjum pt. Farmakologiczny wpływ na dysfunkcje śródbłonka Kraków 02-04.06. 2002
Sygnatura GBL: 735,463

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

827/2523

Tytuł oryginału: Molecular epidemiology in preventive medicine : achievements and new challenges : book of abstracts
Jagiellonian University Collegium Medicum, Department Epidemiology and Preventive Medicine in Krakow
Źródło: - Kraków, UJ CM 2002, 182, [1] s. : il., tab., 24 cm. - Konferencja pt. Epidemiologia molekularna w medycynie zapobiegawczej - osiągnięcia i nowe cele Kraków 20-22.06. 2002
Sygnatura GBL: 735,469

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

828/2523

Tytuł oryginału: Acute renal failure in patients with rhabdomyolysis.
Autorzy: Sułowicz Władysław, Walatek Bogusław, Sydor Antoni, Ochmański Władysław, Miłkowski Andrzej, Szymczakiewicz-Multanowska Agnieszka, Szumilak Dorota, Kraśniak Andrzej, Łonak Hanna, Wójcikiewcz Tadeusz
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR24-CR27, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Rhabdomyolysis is a relatively rare, not alwayd diagnosed causes of acute renal failue (ARF). This fact motivated us to present the results of ARF treatment in the course of this polyetiological clinical syndrome. Material/Methods: The analysis was performed on 84 patients (6 F, 78 M) ranging in age from 18 to 82 years (mean 46.5), in whom rhabdomyolysis was diagnosed based on clinical manifestation and laboratory test results (CPK, GTP, GOT, LDH). Results: The most frequent cause of rhabdomyolysis was alcoholic intoxication (41 patients), often accompanied by hypothermia (15 patients) or trauma (30 patients) isoalted trauma was found in 30 patients, epileptic seizure in 5, and physical exercise in 1 case. In 17 patients, besides alkohol consumption, trauma or epileptic seizure, the use of tranquilizers, anticonvulsants, or narcotic drugs was additonally noted. 78 patients developed ARF requiring dialysis therapy; 49 patients recovered, 5 required maintenance dialysis, and 30 died. Conclusions: During the initial phase of ARF in the course of rhabdomyolysis dynamic increases in serum urea and creatinine were observed, as well as a tendency to hyperkalemia. The treatment results and mortality rate in our study group were primarily influenced by the patients' general condition at admisssion, as well as the extent of organ damage caused by the primary etiological factor. Favorable treatment results were obtained especially in those patients who were hospitalized in a nephrological center, while the worst outcomes were noted in those patients dialyzed in intensive care units, most with multiple trauma.

829/2523

Tytuł oryginału: The influence of controlled hypotension on splanchnic mucosal perfusion using gastric tonometry in patients undergoing resection of meningioma.
Autorzy: Pakulski Cezary, Nowicki Rafał, Kowalczyk Piotr, Bąk Piotr, Mikulski Krzysztof, Badowicz Beata
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR28-CR30, tab., bibliogr. 9 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The aim of our research was to evaluate the influence of general anesthesia with controlled nitroglycerin-induced hypotension on splanchnic mucosal perfusion using gastric tonometry. Material/Methods: In six patients (1 female, 5 males, median age 46 years, range 32 - 62) undergoing elective resection of meningiomas, measurements of gastric mucosal perfusion were taken by nasogastric tonometry. Controlled hypotension was initiated after craniotomy and terminated prior to hemostasis procedures. The mean arterial blood pressure (MAP) was maintained 25 - 30 p.c. below initial parameters during controlled hypotension. The adequacy of perfusion was defined by the intramucosal pH (pHi) and the difference between arterial and intramucosal pH (pHGAP). All parameters were analyzed before the induction of controlled hypotension, after the 1st, 2nd, and 3rd hour, and 1 hour after the termination of controlled hypotension. Results: The MAP decreased during controlled hypotension by ca. 26.5 p.c. (min. 21.1 p.c.; max 31.6 p.c.). The lower MAP was accompanied by a statistically significant increase in heart rate. A significant increase from baseline in regional prCO2 was seen after teh 1st, 2nd, and 3rd hour of controlled hypotension. Intramucosal pH values decreased significantly during the same period, from 7.428 ń 0.032 to 7.372 ń 0.015. Despite these statistically significant differences during controlled hypotension, the pH, values were always greater than 7.35, and the pHGAP values were lower than 0.05, which were recognized as being within the normal range. Conclusions: Controlled hypotension allows accurate evaluation of the blood flow quality at the level of microcirculation. Mild controlled hypotension slightly alters splanchnic mucosal perfusion.

830/2523

Tytuł oryginału: Prevalence of cryoglobulinemia in patients with chronic HCV infection.
Autorzy: Liakina Valentina, Speiciene Danute, Irnius Algimantas, Naraskeviciene Jolanta, Barakauskiene Ausrine, Semuchiniene Teresa
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR31-CR36, tab., bibliogr. 31
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of our research was to determine the prevalence of cryoglobulins in patients with chronic hepatitis C (CHC) at differnt levels of activity and stage of fibrosis and to identify their association with the extrahepatic clinical manifestations. Material/Methods: 87 patients with CHC were investigated for the presence of cryoglobulins. Cryocrit was measured by the Weiner method. Anti HCV, HCV RNR, conventional biochemical tests and liver biopsy were also performed. Results: Cryoglobulins were found in a total of 44 patients: 16 from Group I, II from Group II, and 17 from Group III. A low level of cryoglobulinemia (Cg) (cryocrit ó 2 p.c.) was detected in 16 patients: 9 from Group I, 3 from Group II, and 4 from Group III. Moreover, a high Cg (cryocrit 5 - 10 p.c.) or very high Cg (cryocrit 10 p.c.) was found in a total of 13 patients: 1 patient from Group I, 3 from Group II, and 9 from Group III. Cryo positivity was found in 5 patients with F 1 - 2, in 13 with F 2 - 3, in 4 with F 3 - 4, and in 17 with F 4. Conclusions: Our findings confirmed the high prevalence of Cg in CHC patients in Lithuania, a clear association between Cg and the stage of fibrosis, teh higher prevalence of Cg in cirrhotic patients, and the occurrence of several typical extrahepatic manifestations. The impact of Cg on the course of chronic hepatitis C is not clearly understood; therefore, further studies are needed to clarify this issue.

831/2523

Tytuł oryginału: Comparative study of early Lyme disease: Erythema migrans in New York State and Northeastern Poland.
Autorzy: Zajkowska Joanna, Hermanowska-Szpakowicz Teresa, Coyle Patricia, Ostrowska Jolanta, Pancewicz Sławomir, Kondrusik Maciej
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR37-CR43, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The objeactive was to compare the clinical features of early local Lyme Disease: Erythema Migrans (EM) baeatween two highly endemic areas: New York State and Northeastern Poland. Study was based on self reporting questionnaires, physical examination, and laboratory results. Material/Methods: Group I (n = 27) consissted of citizens from New York State presenting with EM, referred to the study by their physicians. Group II (n = 25) consisted of patients treated as outpatients in 1998 - 1999 at the Department of Infectious Diseases and Neuroinfections, Medical University of Białystok, Poland. The diagnosis of EM was based on patient history and clinical picture. Results: Depending on the immune reaction of the host, the clinical picture is variabale. The initial symptoms range from discrete to strongly expressedd. The inflammatory reaction at the site of tick-bite seems to be milder in Europe than in North America, which is reflected in teh prevalence of the annular form of rash and milder expression of general symptoms. Mildor absent general symptoms and the prevalence of the annular over homogenous form are observed in those areas of Europe where Ixodes ricinus is the main veactor. In the endemic area of New York State, where Ixodes scapularis mainly occurs, uniform EM with fluplike symptoms is more common. Conclusions: There are distinct differences in the typical clinical picures between the two separated endemic areas of New York State and Northeastern Poland, due to different vectors (salive components?), bacaterial genospecies, likely intermediate hosts and clinatic differences. Familiarity with the presenting form of the disease can be very important, especially for primary care physicians.

832/2523

Tytuł oryginału: Extended-spectrum beta-lactamase-producing Enterobacteriaceae strains in community-acquired bacteremia in Southern Israel.
Autorzy: Borer Abraham, Gilad Jacob, Menashe Galia, Peled Nechama, Riesenberg Klaris, Schlaeffer Francisc
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR44-CR47, tab., bibliogr. 21 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: In recent years, extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have emerged in many hospitals worldwide. The increasing dissemination and long-term carriage of these organisms within the community carry tremendous implications on the empirical therapy of community-acquired infection. Material/Methods: To evaluate the prevalence and clinical features of community-acqured bacteremia involving ESBL-producing Enterobacteriaceae in sousthern Israel (ESBL-P) we retrospectively studied all Enterobacteriaceae bacteremias during an 8-month period in the Negev region using medical and laboratory records. Antibiotic susceptibility was determined using teh disk-diffusion method. ESBL production was determined using an E-test ESBL strip. Cases involving ESBL-P were compared to those involving non-producing strains (ESBL-NP) using the ch-square test. Results: In all, 187 Enterobacteriaceae bacteremias were de tected, of which 119 were community-acquired (63.6 p.c.). ESBL-P were found in sic cases (5 p.c.) which are d es c ribed herein in greater detail. Patients with ESBL-P bacteremia were older, and were more likely to stay in the intensive-care unit. Urinary catherterization and bed-ridden contidions were significant risk factorsfor ESBL-P. ESBL-P strains were significantly resistant to nearly all antibiotic agents except of imipienem and piperacillin-tazobaectam as opposing to ESBL-NP. Patients with ESBL-Pbacteremia were more likely to suffer from complications and had a higher mortality. Conclusions: This paper is the first to describe community-acquired Enterobaacteriaceae bacteremia involving ESBL-P strain in Israel. Although the exact prevalence of these organisms in Israel is currently unknown, our findings suggest that ESBL-producers have already begu to disseminate in our community.

833/2523

Tytuł oryginału: Significance of the evaluation of the tissue proliferation specific marker (TPS) and the carcinoembryonal antigen (CEA) in patients with ulcerative colitis and adenocarcinoma.
Autorzy: Krauss Hanna, Ignyś Iwona, Kopczyński Zygmunt, Piątek Jacek
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CR48-CR51, tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Background: The aim of our research was to evaluate TPS concentration as a marker of proliferataive processes and the levels of CEA in children and adults with ulcerative colitis (UC) and in adults with colerctal adenocarcinoma (AC), to find out if there is any interdependence between TPS and C EA concentrations in these patients and to evaluate the usefulness of TPS as a marker of enoplastic risk in patients with UC. Material/Methods: The study was carried out in 3 groups of patients: Group I consisted of 15 children, ages 10 - 18, treated fro UC; Go;rup II, 22 adults, ages 23 - 40, treated for UC; and Group III, 14 patients, aged 40 - 60, in whom AC was diagnosed. Results: In Group II, the mean TPS concentration was significantly higher than in Group I (p 0.00001). In Group III, adults with AC, the mean TPS concentration was 1074.00 ń 1356.87 U/I. Thus there was a statistically significant difference between TPS concentrations in adults with UC and adults with AC (p 0.00005). THere was no statistically significant difference between CEA levels in children and adult groups with UC. However, a statistically significant difference occurrd between CEA concentrations in children and adults with UC and the group of adults with AC (p 0.00001). Conclusions: It would appear that testing for TPS concentration in patients with UC is helpful in monitoring the proliferative process, wheras in patients with neoplastic processes it serves as a screening examination, enabling early detection of recurrences, full evaluation of neoplastic growth, and evaluation of therapeutic effcectiveness.

834/2523

Tytuł oryginału: Detecting the replication of the hepatitis B virus using the ImmunoMax technique following treatment with Interferon-alpha in children with chronic hepatitis.
Autorzy: Kasprzak Aldona, Wysocki Jacek, Zabel Maciej, Surdyk-Zasada Joanna
Źródło: Med. Sci. Monitor 2002: 8 (1) s.PR1-PR7, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Children with HBV in Poland are treated with preparations of interferon-alpha (IFN-ŕ). The continuing laack of complete response to this type of anti-viral therapy remains to be explained. The application of cell biology techniques to identify the viral components in situmakes it possible to clarify the association between the distribution of the virus and morphological injury to the liver, the immune response of the host, and clinical symptoms in the natural course of infection. Our study was intendent to evaluate HBV expression in liver biopsies taken an average of two years after completion of IFN-ŕ therapy in 10 children with serological markers of persistent HBV infection. Materil/Methods: For the immunocytochemical detecion of HBcAg and for the hybridocytochemical detection of HBV-DNA, the avidin-biotin-peroxidase (ABC) technique was employed, as well as classical in situ hybridization, both additionally amplified using the ImmunoMax technique. HBcAg and HBV-DNA levels were estimated using a semiquantitative technique. Resuslts: Our study demonstrated persistent active replication of HBV in the liver in all examined children. A mixed pattern of HBcAg localization prevailed (noted in cell nuclei, cytoplasm and cell membranes) with a somewhat lower proportion of involved cells and a more evident membrane localization of HBcAg, as compared to results obtained before treatment. HBVDNA was observed in the cytoplasm of a fraction of hepatocytes similar to that noted before therapy. Conclusions: The ImmunoMax technique was found to be highly suitable for in situ monitoring of HBV replication after termination of IFN-ŕ treatment. Children with focal distribution of HBcAg andHBV-DNA have the potential for earlier eradication of the virus from their livers.

835/2523

Tytuł oryginału: Chronic mesenteric ischemia: diagnosis and treatment with balloon angioplasty and stenting.
Autorzy: Pietura Radosław, Szymańska Anna, El Furah Majda, Drelich-Zbroja Anna, Szczerbo-Trojanowska Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (1) s.PR8-PR12, il., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The goal of our research was to evaluate the efficacy of endovascular treatment in stenosis of the superior mesenteric artery and the celiac trunk. Material/Methods: During the period 1996-2001, 6 pateints (1 woman, 5 men, ages 46 to 73) were referred to our department with abdominal angina. Angiograms were performed in order to establish the reasons. All these patients presentedd with postprandial abdominal pain and weight loss. Angiography showed stenosis in the superior mesenteric artery in 2 patients, in the celiac trunk in 1 patient, and in both vessels in 3 patients. Typical balloon angioplasty (PTA) was performed with a balloon catheter, 5 - 8 mm in diameter, inflated up to 18 atm. If more than 30 p.c. stenosis remained after PTA, the patient was refered for stenting. Results: In 5 of the 6 patients (83 p.c.) PTA was successful. In one patient (17 p.c.) with poor results from PTA, a Perflex stent (diameter 7 mm) was inplanted in the superior mesenteric artery. The follow-up protocol included clinical and Doppelr ultrasonographic examination at 6, 12, and 18 months after surgery. A good clinical and ultrasound outcome was found in 6 patients at 6 and 12 months, and in 4 patients at 18 months. Conclusions: Balloon angioplasty and stent placement sem to be efficient and safe methods of treatment for abdominal angina in stenosis of the superior mesenteric artery and celiac trunk.

836/2523

Tytuł oryginału: Comparative study of cefaclor AF vs cefuroxime axetil in acute exacerbations of chronic bronchitis.
Autorzy: Haczyński Józef, Chyczewska Elżbieta, Grzelewska-Rzymowska Iwona, Małolepszy Józef, Marcinowska-Suchowierska Ewa, Milanowski Janusz, Oklek Kazimierz, Płusa Tadeusz, Słomiński Marek, Szmygin Katarzyna, Rek Mirosław
Źródło: Med. Sci. Monitor 2002: 8 (1) s.PI1-PI7, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: Acute exacerbations of chronic bronchitis (AECB) are the most common complication of chronic bronchitis. The majority of AECB are caused by infection. The choice of appropriate antibacterial therapy for AECB is becoming more difficult and is usually empirically, Cefaclor and cefruoxime are used for ambulatory treatment of AECB. Material/Methods: This multicenter, randomized, single blind study was undertaken in order to compare efficacy and safety of cefaclor AF (500 mg BID) and cefuroxime axetil (250 mg BID) in 10 days treatment of ambulatory patients with SECB. 170 adults were enrolled into the study. Clinical responses were assessed on 17th - 24th day after randomization. Results: Both antibiotics had high over 97 p.c. effectiveness in the treatment of AECB. There was stastically 1.7 times higher rate of patients with cough release after cefaclor treatment compared to cefuroxime (p 0.03). There was a significantly 2.25 higher rate of patients with AECB symptoms release like: increasing dyspnea, sputum volume and sputum purulence or cough in cefaclor group compard to cefuroxime (p 0.0187). Both treatment resulted in significant improvement of pulmonary peak expiratory flow (PEF). There were no differences between the rates of gastrointestinal and other side effects in both groups. Conclusions: 1. Cefaclor and cefruroxime have similar high efficacy and safety in 10 days treatment of patients with AECB. 2. Cefaclor treatment significantly higher 2.25 times reduces the rate of principle symptoms of AECB compared to cefuroxime. 3. Both antibiotics treatment significantly increase PEF, with higher tendency observed in after cefaclor treatment.

837/2523

Tytuł oryginału: Religious sensitivity pitted against the need to know: autopsy of Jewish children in Israel.
Autorzy: Abraham Ron Ben, Weinbroum Avi A., Kassem Riad, Barzilay Zohar, Paret Gideon
Źródło: Med. Sci. Monitor 2002: 8 (1) s.SR1-SR4, bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The aim of the study was to compare autopsy findings with antemortem findings in children who died in a pediatric intensive care unit. Material/Methods: Consecutive series of patietns who died in the pediatric intensive care unit during 2-year period were used. The study was conducted as a retrospective chart review at community, regional-referral, university-affiliated tertiary medical center of 1200 bed in Israel. Results: Permission was given to perform autopsies on only 10 children (23.8 p.c., mean age 85.7 months) out of the 42 who died during the study period. The mean length of stay in the pediatric intensive care unit prior to death was 13.3 days. Cardiac or hemato-oncologic diseases comprised the major pre-admission diagnoses. The autopsy revealed a major finding that, if known before death, would have altered clinical management in 50 p.c. of the patients: pneumonia, pneumonitis as well as intestinal perforation or necrosis. No correlation was found between patient length of stay in the intensive care unit and the autopsy disclosed information. Conclusions: Our findings suppert the importance of autopsy assessment in the pediatric intensive care setup. We beliewve that postmortem examination is also essential for improving the quality of pediatric patient care.

838/2523

Tytuł oryginału: Active and passive executive function disorder subsequent to closed-head injury.
Autorzy: Pąchalska Maria, Kurzbauer Henryk, Talar Jan, MacQueen Bruce Duncan
Źródło: Med. Sci. Monitor 2002: 8 (1) s.CS1-CS9, il., tab., bibliogr. 41 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: Executive dysfunction is one of the most destrucive sequelae of closed head injuries (CHI), often impeding or even preventing the patient's return to normal functioning. On the basis of extensive clinical testing of patients with neurobehavioral disturbances resulting from CHI, the authors propose a new typology of executive dysfunction based on the primary behavioral distinction between active ('acting without thinking') and passive ('thinking without acting') forms of executive function disorder. Material/Methods: Two patients were selected for detailed presentation. Both present with mild to moderate motor and cognitive symptoms resulting from closed head injury. The medical histories of the two patients are similar (educated professionals, mid-40s, married with children, injuries suffered in a traffic accident, 2 months in coma) except for the location of focal injuries. Results: Despite considerable progress in rehabilitation, the extent of functional disorder is disproportionately large in comparison to the degree of objective disability measured by standard instruments. It is suggested that the reason for this disparity lies in executive dysfunction. In particular, a model for executive functioning will be presented to explain why and low selective destruction of particular anatomical/functional components leads to the behvioral consequences known as 'executive dysfunction'. Conclusions: executive dysfunction is a distinct clinical syndrome which occurs in at least two distinguishable varieties, active and passive.

839/2523

Tytuł oryginału: The ' CARFEM' vascular index as a predictor of coronary atherosclerosis.
Autorzy: Claessens Philip, Claessens Christophe, Claessens Marc, Claessens Maria, Claessens Jan
Źródło: Med. Sci. Monitor 2002: 8 (1) s.MT1-MT9, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The purpose of the study was detection of coronary atherosclerosis and identification of persons at risk by means of non-invasive examiantion of the carotid and peripheral arteries. Material/Methods: Investigation of 366 patients: 229 males and 137 females divided into groups according to age and the severity of the coronary atherosclerotic lesions. Only patients with a coronary stenosis of mor than 90 p.c., who needed angioplasty or coronary bypass surgery, were included. Group I consisted of patients with nromal coronary arteries; Gorup II, cirtical one-vessel disease; Group III, two-vessedl disease; Gorup IV, critical three-vessel diseases. By means of a vascular duplex scan we measured bilaterally the intima-media thickness of the posterior wall of the common carotid artery. In order to provide a more accurate estimation of the total atherosclerotic burden we have created the 'CARFEM' index (combination index of intima media thickness of carotid artery and total wall thickness of the superficial femoral artery). Results: The anthropometric data were identical among the different groups. There are statistically highly significantly differences in the intima-media thickness of the common carotid artery when patients with normal coronary arteries are compared with patients with three-vessel coronary disease. By calculating the 'CARFEM' index not only we can differntiate between patients with normal coronary arteries and patients with critical three-vessel disease, but there are also ways to differentiate between different subgorups and to assess possible one-vessel...

840/2523

Tytuł oryginału: Evaluation of the usefulness of the ELISA method for detection of enterovirus antibodies in serum samples of patients with myocarditis.
Autorzy: Wielkopolska Anna, Gut Włodzimierz, Binduga-Gajewska Iwona, Jarząbek Zdzisław
Źródło: Med. Sci. Monitor 2002: 8 (1) s.MT10-MT14, il., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Enteroviruses are among the etiological factors in myocarditis, In the majority of cases it is impossible to obtain a direct confirmation of the presence of the virus, and typologically specific methods are of limited applicability, due both to the number of enteroviruses that can cause myocarditits, and to the serological cross reactions among them. The goal of our research was to determine the usability of the ELISA test and the complement fixation reaction (CFR) in diagnostic testing of the serum of patients with myocarditis. Material/Methods: The research material consisted of 1197 serum samples collected from patients with myocarditis. In all these samples we measured the contentration of IgG adn IgM enterovirus antibodies using the ELISA method, and teh height of the titer using the CFR method. Results: The presence of IgM antibodies is a confirmation of current infection. A postitive result was obtained for 61 p.c. of the patients. On the other hand, if one accepts as confirmation of infection a titer significantly different from the level observed in thy healthy population, namely ň 16 for CFR and ň 11 for ELISA-IgG (cf. positive results of 33 p.c. for CFR and 18.8 p.c. for ELISA IgG in our patients), the results may be disproportional. If one assumes as a test criterion a significant increase in titer, the presence of infection was confirmed in 28.5 p.c. of cases for CFR and 62 p.c. for ELISA-IgG. Conclusions: An analysis of the concentration of IgG antibodies constitutes a clear confirmation of infection., if the criterion of evaluation is based on a measure in a single serum sample. In this case it is necessary to test for a significant increase in the titer. The use of the average titer for the population at large to evaluate results leads to both false positives and false negatives. Testing for IgM antibodies in a single serum sample is a faster method which confirms the current presence of infection. Our results indicate that the ELISA test can be used to diagnose myocarditis, since

841/2523

Tytuł oryginału: Prediction of the biomechanical properties of cancellous bone using ultrasound velocity and bone mineral density - an in vitro study.
Autorzy: Drozdowska Bogna, Pluskiewicz Wojciech, Przedlacki Jerzy
Źródło: Med. Sci. Monitor 2002: 8 (1) s.MT15-MT20, tab., bibliogr. 23 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Backgrouns: Recent experimental studies have suggested that the combination of DXA (dual energy X-ray absorptiometry) and ultrasound (US) measurements could improve the prediction of bone strength. The aim of this study was to investigate the ability of bone density and US velocity, measured by the phalangeal US system, to predict the Young's modulus and strength of cancellous bone. Material/Methods: 35 cubes of bovine cancellous bone from the distal radii were prepared using a dimond cutting saw. The US velocity (Speed of Sound - SoS [m/s]) was measured for the three orthogonal directions (proximal-distal -PD; anterior-posterior - AP; medial-lateral - ML) using a DBM Sonic 1200 device (Igea, Italy). Bone mineral density (BMD [g/cmý]) was measured by DXA, as well as the apparent volumetric BMD (v-BMD [g/cm**3], as the ratio of BMD to sample thickness). Young's modulus (E[MPa]) and ultimate strength (omikron[MPa]) were measured in the PD and AP directions by the uniaxial compressive test. Results: The SoS and biomechanical parameters were significantly higher (p 0.001) for the PD direction than for horizontal directions. In the PD direction, SoS, BMD adn v-BMD correlated significantly with E (r = 0.79 - 0.84, p 0.001) and omikron (r = 0.68 - 0.80, p 0.01). In the AP direction, SoS and v-BMD correlated significantly (r = 0.75 - 0.77, p 0.05) and BMD correlated almost significantly (r = 0.66, p = 0.05) with omikron, whereas their correlations with E were nonsignificant. In the PD direction, teh predictive value of SoS or bone density alone for E and omikron was generally...

842/2523

Tytuł oryginału: An assessment of the effectiveness of magnetic resonance imaging in delayed sequences after administration of Gd-DTPA contrast in the detection of metastatic lesions in the brain.
Autorzy: Grzesiakowska Urszula, Tacikowska Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (1) s.MT21-MT24, il., tab., bibliogr. 8 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Advances in oncology in recent years have made it possible to undertake radical interventions even in advanced cases. Local treatment, surgical or radiosurgical, is applied ever more frequently in cases of metastases to the brain. This requires accurate determination of the number and location of metastases by means of imaging techniques. The goal of our research was to establish whether the use of sequences delayed by 20-30 minutes after gadolinium injection improves the number of metastatic lesions detected in the brain by MRI. Material/Methods: Twenty-eight patients were studied, ranging in age from 24 to 72 years, diagnosed with malignant tumors and suspected metastases to the brain. MRI examinations were performed with a 2T unit in SE T1 immediately after i.v. administration of a 0.1 mmol/kg does of gadolinium, and again 20 - 30 minutes after contrast injection; both sequences were done in axial projection in layers idenitcal as in the SE T1 sequence made befor gadolinium injection. The focal lesions were counted and classified by size. The number of detected lesions was calcualted in each group, comparing early and late phases after contrast injection. Results: The number of all nodules found in the delayed sequences was significantly higher in comparison to the early phase after gadolinium injection. Conclusions: Delayed sequence should be used to supplement basic sequences in the diagnosis of maignant metastases to the brain in selected oncological cases.

843/2523

Tytuł oryginału: Tonal nitric oxide and health - a free radical and a scavenger of free radicals.
Autorzy: Benz Danielle, Cadet Patrick, Mantione Kirk, Zhu Wei, Stefano George B.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.RA1-RA4, bibliogr. 68 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Basal/tonal nitric oxide (NO) production helps maintain particular microenvironments, i.e., vascular. Besides NO's function in controlling the activation state of various tissues such as immune cells, its presence appears to modulate other free radical levels, i.e., H2O2, in these same tissues and indeed these processes may be one and the same. Thus, by being a free radical, along with the ability to scavenge other free radicals. NO is placed in a pivotal regulatory position. We surmise that in the absence of adequate NO release other free radicals may go 'unchecked' and, therefore, initiate tissue damage. Furthermore, under these circumstances, proinflammatory events will occur due to heightened cell sensitivity and a diminished control of NF-kB. In an excess situation, and one without an appropriate cirucmstance, i.e., microbial acation, NO may baecome the harmful agent. Hence, balancing basal NO production in body compartments may represent a fundamental process in maintaining general, long-term health.

844/2523

Tytuł oryginału: Markers of inflammation and coronary artery disease.
Autorzy: Saadeddin Salam M., Habbab Moh'd A., Ferns Gordon A.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.RA5-RA12, tab., bibliogr. 154 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Many reaent experimental and clinical studies have provided evidence for the presence of inflammation in atherosclerotic lesions. Onging inflammatory reactions within coronary atherosclerotic plaques are increasingly thought to be crucial determinants of the clinical course of patients with coronary aertery disease (CAD). These facts lead to a search for reliable markers reflecting the inflammatory process in the atherosclerotic plaques. Circulating markers may consist of cytokines directly rleased from inflammatory cells present in the palques and tissues exposed to recurrent ischemia as well as other reactants produced in response to these cytokines such as adhesion molecules and acute phase proteins. Recent studies suggest that markers of inflammation may reflect different aspects of the atherothrombotic process at different points in the continuum of acute coronary syndromes, have a potential role for the prediction of risk for developing CAD, adn may correlate with severity and future risk for CAD. In spite of these findigns, the clinical utility of measuring these markers is limited by the availability of reproducible diagnostic test assays. In addition, it remains to be determined whether markers of inflammation actually have a causal relation with cardiovascular disease, or simply reflect the underlying disease proces. Such determination becomes important with the potential use of these markers in targeting preventive therapies. Therefore, further well-designed prospective evaluation of each of these markers is needed before their use in routine practice.

845/2523

Tytuł oryginału: Thrombolysis research - new objectives after a shift of accent.
Autorzy: Maksimenko Alexander V.
Źródło: Med. Sci. Monitor 2002: 8 (1) s.RA13-RA21, il., tab., bibliogr. 76 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The methods of increasing the efficacy of thrombolysis under clinical and experimental conditions are have been discussed in the light of analysis of current tendencies in the investigation and development of thrombolytic and adjunctive therapy. Analysis of the data obtained in the search for novel plasminogen activators for thrombolytic enzyme therapy has shown a decrease and depletion of this search. The reserves for further increase in thrombolysis effciency have been notified. Special attention is paid to the purposes, realms and problems of adjunctive therapy, which is a promising breakthrough area in the effort of incrasing the efficacy of trhombolysis.

846/2523

Tytuł oryginału: Use of secretin in the treatment of childhood autism.
Autorzy: Kamińska Barbara, Czaja Mariusz, Kozielska Ewa, Mazur Elżbieta, Korzon Maria
Źródło: Med. Sci. Monitor 2002: 8 (1) s.RA22-RA26, bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The paper presents current views concerning childhood autism. The authors present the concepts of etiology of this disorder, emphasizing the role of negative psychical stimuli in early childhood and the role of mother's contact with the child. Organic factors, including genetic background, developmental abnormalities of the nervous system, teratogenic factors and perinatal traumas are also taken into consideration. The role of metabolic factors and enterohormones, particularly thoses belonging to the secretin group and their effect on the function of the gastrointestinal tract and central nervous system is emphasized. We discuss signs which may be indicative of first symptoms of autism in different age groups. A typical symptom of autism is no development of speech, observed from infancy, taking the form of complete mutism at later stages. It has been emphasized that most pathologic symptoms result from altered perception of external stimuli, which arouse fear and anxiety. Autistic patients may suffeer from gastrointestinal tract disturbances such as abadominal pains and diarrhea. Methods used hitherto in the therapy of childhood autism, mainly by psychologists and psychiatrists, as well as some attempts of pharmcological treatment, are presented. The structure and function of secretin, as well as its effects on the motor and secretory function of the stomach and the exocrine function of the pancreas are discussed. The role of secretin in diagnostic tests, among others in the diagnosis of gastrinoma, is emphasized. We also present the history of the application of secretin in the therapy of childhood autism.

847/2523

Tytuł oryginału: Expression of TGF-á type I and II receptors in normal cancerous human endometrium.
Autorzy: Piestrzeniewicz-Ulanska Dagmara, Brys Magdalena, Semczuk Andrzej, Jakowicki Jerzy A., Krajewska Wanda M.
Źródło: Cancer Lett. 2002: 186 (2) s.231-239, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 306,023

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Transforming growth factor-á (TGF-á) belongs to a superfamily of structurally related polypeptides involved in various biological processes, including cell growth, proliferation and differentiation, angiogenesis, apoptosis, and extracellular matrix remodeling. We tried to define the different expression patterns of the TGF-á receptors by investigating the female reproductive organs during the menstrual cycle and endometrial tumorigenesis, because their role in these processes is still unclear. In this study, we examined the expression of the TGF-á type I and type II receptors in normal (n = 13) and carcinomatous (n = 42) endometrial tissue specimens using reverse transcriptase polymerase chain reaction and immunological (Western blot and enzyme linked immunosorbent assay) methods. Two uncommon female genital tract tumors, rhabdomyosarcoma of the uterine cervix and uterine carcinosarcoma, were also included. There were no significant differences between normal and cancerous endometrial tissues regarding the TGF-á receptors mRNA levels. However, we observed a markedly low TGF-á type I receptor protein level (P 0.028; Mann-Whitney-U test), while the malignant endometrium showed a significantly higher TGF-á type II receptor protein level (P 0.007; Mann-Whitney-U test) than the normal endometrium. Moreover, significantly elevated TGF-á receptor type II protein level was noted when depth of myometrial invasion of endometrial carcinomas was considered (P 0.05; Mann-Whitney-U test). In contrast to uterine carcinosarcoma, in which no detectable mRNA for TGF-á type II...

848/2523

Tytuł oryginału: The influence of a new vanadium compound, Bis(2,2'-bipyridine)oxovanadium(IV) sulphate on liver Golgi complexes from control and streptozotocin-diabetic rats.
Autorzy: Kordowiak A. M., Dąbroś W., Kajda B.
Źródło: Horm. Metab. Res. 2002: 34 (10) s.556-560, il., tab., bibliogr. 32 poz.
Sygnatura GBL: 312,226

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Among the previously studied organic vanadium derivatives showing anti-diabetic action, we investigated a new complex, bis(2,2'-bipyridine)oxovanadium(IV) sulphate. We tested its ability to normalise parameters previously described for streptozotocin (STZ)-diabetes, such as lower yields of Golgi-rich membrane fraction isolation, decreased activity of Golgi membrane marker enzyme - galactosyltransferaze(GalT) - and altered morphology of rat liver Golgi complexes. Oral application as a drinking solution of 1,8 mmol bis(2,2'-bipyridine)oxovanadium(IV) (dissolved in 0.09 M NaCl) caused a similar dispersion of GalT activities in both vanadium treated groups, control and diabetic. Very low activities of the enzyme (characteristic for untreated diabetes) we found only in approximately 35 p.c. of the STZ-diabetic rats treated with the new vanadium compound. The morphology of liver Golgi complexes in diabetic rats treated with bis{2,2'-bipyridine)oxovanadium(IV) sulphate was improved, which manifested itself in the reappearance of vacuoles with VLDL and coated and uncoated secretory vesicles. In view of biochemical and morphological parameters of normalised diabetic rat liver Golgi apparatus, the new vanadium complex was more effective than bis(oxalato)oxovanadium(IV) or bis(kojato)oxovanadium(IV), but in our experimental model, the best anti-diabetic, orally applied drug was the bis(maltolato)oxovandium(IV) previously investigated.

849/2523

Tytuł oryginału: Carcinoembryonic antigen, squamous cell carcinoma antigen, CYFRA 2I-I, and neuron-specific enolase in squamous cell lung cancer patients.
Autorzy: Kulpa Jan, Wójcik Ewa, Reinfuss Marian, Kołodziejski Leszek
Źródło: Clin. Chem. 2002: 48 (11) s.1931-1937, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 304,722

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag), and CYFRA 21-1 are the most useful markers for non-small cell lung cancer (NSCLC), but neuron-specific enolase (NSE) is a tumor marker of choice for SCLC. The determination of NSE in NSCLC could allow selection of patients with neuroendocrine features. NSCLC patients whose tumors have neuroendocrine properties may be more responsive to chemotherapy; however, these tumors have been reported to be more aggressive. Tumor markers are not suitable for diagnosis; their principal applications are in monitoring of therapy and prognosis. Methods: Tumor markers were measured in 200 untreated patients with squamous cell lung cancer (SQC) and a reference group (n = 220; 124 healthy persons and 96 patients with nonmalignant lung disease). CEA and SCC-Ag were measured by microparticle enzyme immunoassays on Abbott AxSYM and IMx analyzers. CYFRA 21-1 and NSE were measured by electrochemiluminescence immunoassays on the Roche Elecsys 2010. Results: CEA, SCC-Ag, CYFRA 21-1, and NSE were increased above the cutoffs in 26 p.c., 32 p.c., 67 p.c. and 28 p.c. of tested patients, respectively. The area under the ROC curve for CYFRA 21-1 was higher than those for CEA, SCC-Ag, and NSE (SQC vs controls). CYFRA 21-1 and CEA were significantly higher in advanced SQC than in early stages of disease (P 0.0001 and P 0.0004, respectively). In multivariate analysis of survival, CYFRA 21-1 was an independent but nonspecific prognostic factor in the operable group of SQC patients, whereas NSE was an independent...

850/2523

Tytuł oryginału: Synthesis and biological activity of strongly fluorescent tricyclic analogues of acyclovir and ganciclovir.
Autorzy: Goslinski Tomasz, Golankiewicz Bożenna, De Clercq Erik, Balzarini Jan
Źródło: J. Med. Chem. 2002: 45 (23) s.5052-5057, il., tab., bibliogr. 13 poz.
Sygnatura GBL: 312,133

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In search of strongly fluorescent tricyclic analogues of acyclovir (ACV, 1) and ganciclovir (GCV, 2) derivatives of the 3,9-dihydro-9-oxo-5H-imidazo[1,2-ŕ]purine system, several 6-[4-(acyloxy)-phenyl], 6-[4-(acylamino)phenyl], and 6-[4-(phenoxycarbonyloxy)phenyl]-substituted TACV and TGCV analogues were synthesized and evaluated for their activity against herpes simplex virus types 1 and 2 in cell culture. All TACV and TGCV analogues showed strong fluorescence (quantum yield of 30-65 p.c. vs 2-aminopurine 100 p.c.). The 6-[4-(phenoxycarbonyloxy)phenyl]-substituted compounds 11 and 19 displayed the best combination of the fluorescence and antiviral potency.

851/2523

Tytuł oryginału: Fatal outcome of a hypersensitivity reaction to paclitaxel: a case report.
Tytuł polski: Śmierć pacjenta w wyniku wystąpienia reakcji nadwrażliwości na paklitaksel - opis przypadku.
Autorzy: Kloover Jeroen S., Bakker Michael A. den, Meerbeeck Jan P. van
Źródło: Współcz. Onkol. 2002: 6 (8) s.486, 488-489, bibliogr. 11 poz., streszcz.
Sygnatura GBL: 313,382

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: Paklitaksel jest powszechnie stosowany w leczeniu, m.in. raka jajnika, piersi oraz niedrobnokomórkowego raka płuc. Przed zaaplikowaniem leku należy go najpierw rozpuścić w substancji Cremophor EL, będącej pochodną oleju rycynowego. Zarówno sam paklitaksel, jak i jego rozpuszczalnik mogą wywoływać reakcje nadwrażliwości u leczonych tym lekiem chorych. Premedykacja polegająca na zastosowaniu kortykosteroidów może zapobiec wystąpieniu reakcji nadwrażliwości na paklitaksel. Powszechnie stosowany deksametazon jest długo działającym preparatem kortykosteroidowym, o okresie półtrwania wynoszącym ok. 48 godz. Lek ten hamuje reakcje zapalne, obniża stężenie mediatorów reakcji zapalenj, takich jak prostaglandyny i cytokiny oraz osłabia wrażliwość naczyń krwionośnych na czynniki o działaniu naczynioskurczowym. Przed rozpoczęciem leczenia paklitakselem stosuje się jeden z dwóch schematów premedykacji. Pierwszy z nich polega na doustnym podaniu 20 mg deksametazonu na 12 i 6 godz. przed rozpoczęciem każdego cyklu chemioterapii. Drugi, nie wymagający współpracy chorego, polega na dożylnym podaniu 5-20 mg deksametazonu bezpośrednio przed każdym cyklem. Pomimo tego, że pełna aktywność biologiczna deksametazonu pojawia się dopiero po kilku godzinach od jego podania, obie metody premedykacji uznawane są za równie skuteczne w zapobieganiu reakcjiom nadwrażliwości po podaniu Pakltakselu [2, 9, 11]. W niniejszym artykule opisano przypadek 52-letniego mężczyzny leczonego paklitakselem, u którego w górnym płacie lewego płuca wykryto naciekające śródpiersie ognisko raka niedrobnokomórkowego oraz liczne przerzuty w obrębie opłucnej...

852/2523

Tytuł oryginału: English language program : alumni 2002
Opracowanie edytorskie: Kucharz Eugeniusz J. (ed.).
Medical University of Silesia in Katowice
Źródło: - Katowice, MUS 2002, 44 s. : il., 21x21 cm.
Sygnatura GBL: 660,767

Typ dokumentu:

tytuł obcojęzyczny

Temat korporatywny:

Śląska Akademia Medyczna w Katowicach

853/2523

Tytuł oryginału: Novel competitive PCR methods for quantitation of T-cell receptor delta (TCRD) gene rearrangements.
Autorzy: Masternak Michał M., Przybylski Grzegorz K., Smoczkiewicz Paweł, Płotek Włodzimierz, Kowalczyk Dariusz W., Nowak Jerzy S.
Źródło: J. Appl. Genet. 2002: 43 (2) s.235-244, il., tab., bibliogr. s. 242-244
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Since the invention of the polymerase chain reaction (PCR) several quantitative PCR-based approaches have been described. Recently, the real-time PCR method became a standard in quantitative PCR, althrough high costs of the necessary equipment and reagents make it unaffordable for many laboratories. In this paper we describe to novel competitive PCR techniques, which were used to determine the frequency of T-cell receptor delta gene (TCRD) rearrangements in peripheral blood leukocytes. In the referrence gene competitive PCR (rgc-PCR) the rearranged TCRD gene competes with the reference gene (RAG1) for common reagents (dNTPs and Taq polymerase). The intensity ratio of amplification products, TCRD/RAG1, corresponds to the portion of cells containing a rerrangement. A series of reactions was performed, in which RAG1 primers were added to the PCR after different numbers of cycles. On the basis of the number of cycles needs to obtain equal band intensity, the frequency of cells containing a rerrangement was calculated. In the common primer competitive PCR (cpc-PCR), two gene rearragements. Vë1-Jë1 and Vë2-Jë1, compete for the common Jë1 primer. The competing genes are amplified from the same genomic DNA template; therefore unlinke in the method using the internal competitor, the results are not affected by the quantity of quality of the analysed sample. We showed that the rgc-PCR and cpc-PCR are reliable ang give reproducible results. The methods do not require any expensive equipment or reagents, and can be used to determine the frequency of gene rearrangement.

854/2523

Tytuł oryginału: The KVLQT1 gene is not a common target for mutations in patients with various heart pathologies.
Autorzy: Moric Ewa, Herbert Ernest, Mazurek Urszula, Samelska Justyna, Cholewa Krzysztof, Trusz-Gluza Maria, Wilczok Tadeusz
Źródło: J. Appl. Genet. 2002: 43 (2) s.245-254, il., bibliogr. s. 253-254
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The long QT syndrome (LQTS) is a disorder of ventricular repolarization that exposes affected individuals to cardiac arrythmias and sudden death. The first gene for LQTS has been mapped to chromosome 11 p. 15.5 by genome-wide linkage analysis. This gene, originally named KVLQT1 (and later KCNQ1), is a novel potassium channel gene. Mutations in the human KVLQT1 gene, encoding the ŕ-subunit of the KVLQT1 channel, cause the long QT syndrome. In this work, we analysed the sequence of six KVLQT1 exons in patients with various heart pathologies. We describe 6 different mSSCP patterns with no disease-related SSCP conformes in any sample. Different sequencing of exons 2 to 7 confirmed the absence of mutations. This suggests that the analysed region of the KVLQT1 gene is not commonly involved in pathogenesis of the long QT syndrome.

855/2523

Tytuł oryginału: Variability of the human mitochondrial DNA control region sequences in the Lithuanian population.
Autorzy: Kasperaviciute Dalia, Kucinskas Vaidutis
Źródło: J. Appl. Genet. 2002: 43 (2) s.255-260, il., tab., bibliogr. s. 259-260
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The Lithuanians and Latvians are the only two Baltic cultures that survived until today. Since the Neolithic period the native inhabitants of the present-day Lithuanian territory have not been replaced by any other ethnic group. Therefore the genetic characterization of the present-day Lithuanians may shed some light on the early history of the Balts. We have analysed 120 DNA samples from two Lithuanian ethnolinguistic groups (Aukstaicail and Zemaiciai) by direct sequencing of the first hypervariable segment (HVI) of the control region of mitochondrial DNA (mtDNA) and restriction enzyme digestion for polymorphic site 00073. On the basis of specific nucleotide substitutions the obtained sequences were clasified to mtDNA haplogroups. The revealed the presence of almost all European haplogroups (except X) in the Lithumanian sample, including those that expanded through Europe in the Palaeolithic and those whose expansion ocurred during the Neolithic. Molecular diversity indices (gene diversity 0.97, nucleotide diversity 0.012 and mean number of pairwise differences 4.5) were within the range usually reported in European populations. No significant differences between Aukstaiciai and Zemaiciai subgroups were found, but some slight differences need further investigations.

856/2523

Tytuł oryginału: Immunotherapy of cancer through targeting of the p53 tumor antigen.
Autorzy: Zwaveling Sander, Burg Sjoerd H. van der, Menon Anand G., Melief Cornelis J. M., Offringa Rienk
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.297-305, il., bibliogr. 75 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The expression of the p53 tumor suppressor protein is frequently increased in a great variety of human cancers, making this antigen an attractive candidate for targeting therapeutic T cell immunity. However, potential complications as a result of immunological tolerance or autoimmune pathology must be taken into account when exploiting this ubiquitously expressed auto-antigen for the immunotherapy of cancer.

857/2523

Tytuł oryginału: Lymphocyte distribution and intrahepatic compartmentalization during HCV infection: a main role for MHC-unrestricted T cells.
Autorzy: Agrati Chiara, Nisii Carla, Oliva Alessandra, D'Offizi Gianpiero, Montesano Carla, Pucillo Leopoldo Paolo, Poccia Fabrizio
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.307-316, il., bibliogr. 103 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Hepatitis C virus (HCV) infection induces an acute and chronic liver inflammation through an immune-mediated athway that may lead to cirrhosis and liver failure. Indeed, HCV-related hepatitis is characterized by a dramatic lymphocyte infiltrate into the liver which is mainly composed by HCV non-specific cells. Several data indicated that interferon (IFN)-ç secretion by intrahepatic lymphocytes 9IHL) may drive non-specific cell homing to the liver, inducing interferon inducible protein-10 9p-10) production. An interesting hallmark of these IHL is the recruitment of lymphocytes associated with mechanisms of innate immunity, such as natural killer (NK), natural killer T (NKT) and çë T lymphocytes. CD81 triggering on NK cell surface by the HCV envelope glycoprotein E2 was recently shown to inhibit NK cell function in the liver of HCV-infected persons, resulting in a possible mechanism contributing to the lack of virus clearance and to the establishment of chronic infection. In contrast, intrahepatic NKT cells restricted to CD1d molecules expressed on the hepatocyte surface may contribute to a large extent to liver damage. Finally, an increased frequency of T cells expressing the çë T cell receptor (TCR) was observed in HCV-infected liver and recent observations indicate that intrahepatic çë T cell activation could be directly induced by the HCV/E2 particle through Cd81 triggering. These cells are not HCV specific, are able to kill target cells including primary hepatocytes and their ability to produce T helper (Th)1 cytokines is associated with a higher degree of liver disease...

858/2523

Tytuł oryginału: Immunotherapy in the management of sepsis.
Autorzy: Sikora Janusz Piotr
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.317-324, bibliogr. 65 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: This work presents the role of Gram-negative bacteria endotoxins, pro- and anti-inflammatory cytokines and reactive oxygen species (ROS) in the complex and not fully explained pathogenesis of sepsis. The so-called "respiratory burst" of neutrophils and the antioxidant mechanisms of the host are also discussed. The work focuses on possible approaches to the management of sepsis connected with immunotherapy. Neutralization of endotoxin lipopolysaccharide (LPS), anti-tumor necrosis factor ŕ (TNF-ŕ) therapy with monoclonal antibodies or pentoxifylline (PTXF), as well as soluble recombinant cytokine agonists and antagonists used in clicnial trials are taken into consideration. In addition, cytokine manipulation therapy, anti-adhesion techniques, glucocorticoides and antioxidant barrier interference are also described. So far there has been no immunotherapy of sepsis in children of proven clinical efficacy, which promts an aggressive examination of the immune system aimed at affecting its function.

859/2523

Tytuł oryginału: The role of TGF-á signaling in the pathogenesis of fibrosis in scleroderma.
Autorzy: Ihn Hironobu
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.325-331, bibliogr. 86 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Excessive extracellular matrix (ECM) deposition in the skin, lung, and other organs is a hallmark of systemic sclerosis (SSc). The pathogenesis of SSc is still poorly understood, but increasing evidence suggests that transforming growth factor (TGF)-á is a key mediator of tissue fibrosis as a consequence of ECM accumulation in pathological states such as systemic sclerosis. TGF-á regulates diverse biological activities including cell growth , cell death or apoptosis, cell differentiation, and ECM synthesis. TGF-á is known to induce the expression of ECM proteins in mesenchymal cells and to stimulate the production of protease inhibitors that prevent enzymatic breakdown of the ECM. This review focuses on the possible role of TGF-á in the pathogenesis of fibrosis in SSc.

860/2523

Tytuł oryginału: The protective role of the HLA-DR locus in patients with various clinical types of alopecia areata.
Autorzy: Broniarczyk-Dyła Grażyna, Prusińska-Bratoś Magdalena, Dubla-Berner Małgorzata, Arkuszewska Celina, Borowiec Maciej, Kowalski Marek L., Woszczek Grzegorz
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.333-336, tab., bibliogr. 18 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: One of the genetic factors associated with the development of alopecia areata (AA) is the HLA locus. The study comprised 52 patients with AA aged 10 to 64 years. The frequences of HLA-DRB alleles in the patients and controls were compared. The control group comprised 152 healthy persons. Familia occurrence of AA was seen in 7 (13.5 p.c.) cases. A significantly lower frequency of HLA-DRB1*03 was observed in patients with AA comparison with the control group. In all patients with AA, alleles HLA-DRB1*15/*16 occurredd more frequently than in the control group, but this was not significant after correction. In the group of patients with more severe forms of alopecia areata (alopecia areata totalis/alopecia areata universalis) there was no signficant difference in HLA-DR allele distribution.

861/2523

Tytuł oryginału: The lack of relationship between serum content of MBL, sCD14, Anti-PPD and Anti-Hsp65 IgG and ingestion of Mycobacterium bovis BCG bacilli by phagocytes.
Autorzy: Paziak-Domańska Beata, Bonar Agnieszka, Kowalewicz-Kulbat Magdalena, Klink Magdalena, Kowalski Michał, Karhukorpi Jari, Karittunen Ritta, Jurkiewicz Magdalena, Różalska Barbara, Rudnicka Wiesława
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.337-344, il., bibliogr. 28 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Prophylactic vaccination against tuberculosis (TB) with a live attenuated strain of Mycobacterium bovis bacille Calmette-Gurin (BCG) has been used worldwide. However, TB remains one of the most significant diseases of humans and animals. Better understanding of the mechanisms of human immunity to mycobacteria is essential for the development of new vaccines and the estimation of their efficacy. In this study we determined the levels of known humoral mediators of mycobacterial phagocytosis, i.e. mannose-binding lectin (MBL), soluble CD14 (sCD14), antibodies of the immunoglobulin G (IgG) class against mycobacterial purified protein derviative (PPD), and mycobacterial Hsp65 antigen, in the sera from healthy young volunteers vaccinated with BCG and presenting positive and negative Mantoux responses to PPD. Then we asked the question as to whether macrophages and polymorphonuclear leukocytes (PMNs) from the individuals with postiive tuberculin test (TT(+)) and negative tuberculin test (TT(-)) differed in their ability to ingest mycobacteria. We also looked for a relationship between the intensity of mycobacterial ingestion by phagocytes in a medium of autologous sera containing different concentrations of MBL, sCD14 and anti-mycobacterial IgG. We found no significant differences between the investigated paraemters for TT(+) and TT(-) volunteers. Our result suggest that the ability of macrophages and PMNs to ingest mycobacteria depends on an individual, intrinsic capacity of the phagocytes.

862/2523

Tytuł oryginału: Structural and serological characterization of the lipopolysaccharide from Proteus penneri 20 and classification of the cross-reacting Proteus penneri strains 10, 16, 18, 20, 32 and 45 in Proteus serogroup O17.
Autorzy: Sidorczyk Zygmunt, Toukach Filip V., Zych Krystyna, Arbatsky Nikolay P., Drzewiecka Dominika, Ziółkowski Andrzej, Shashkov Alexander S., Knirel Yuriy A.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (5) s.345-350, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proetus penner 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The following structure of the polysaccharide was established: (...). It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups habe not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.

863/2523

Tytuł oryginału: Benefits and menaces related to the use of statins in patients after renal transplantation.
Autorzy: Chmielewski Michał, Zdrojewski Zbigniew, Rutkowski Bolesław
Źródło: Ann. Transplant. 2002: 7 (2) s.6-10, il., tab., bibliogr. 60 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Hypercholesterolemia in patients after renal transplantation composes a signifcant risk factor of cardio-vascular disease, it may also worsen graft survival. Statins are the most potent drugs to lower blood cholesterol. They also posses numerous pleiotropic abilities. The paper presents benefits of the use of statins in patietns after renal transplantation, as well as the dangers related to the side effects of these drugs. It underlines that adequate use of statins is worth considering in patients after renal transplantation, taking into account not only their abilit to lower cholesterol but also their additional properties positively influencing graft survival.

864/2523

Tytuł oryginału: Atrial resynchronization in patients after heart transplantation.
Autorzy: Kutarski Andrzej, Zakliczyński Michał, Oleszczak Krzysztof, Kuśnierz Jacek, Lech Bogdan, Wojarski Jacek, Kalarus Zbigniew, Przybylski Roman, Widomska-Czekajska Teresa, Religa Zbigniew, Zembala Marian
Źródło: Ann. Transplant. 2002: 7 (2) s.11-17, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Lower-Shumway technique (atrioatrial anastomosis) is the most frequently used technique for orthotopic heart transplantation and such a patient has two right atria and two sinus nodes. Sinus node dysfunction (SND) is a frequent finding in pts. after OHT; taking advantage of the frequency of innervated sinus node of recipient's atrial remnant as a natural biosensor for triggered pacing of donor atrium is an interesting option for these pts. The aims of our study: 1. the analysis of possibility of utility of recipient atrial sinus node as natural biosensor for triggered donor atrium permanent pacing in transplanted patients with SND. 2. the evaluation of pacing and sensing conditions of the recipient's atrium in the some pts. 3. long-term observation of effectiveness A2A2T(/D) pacing mode. Methods. 10 out of 37 pts. received A2A2T (8 pts.) and A2A2T/D (2 pts) pacing systems. In pts. with NSR of recipients atrium, we evaluated acceleration of its frequency during slight exercise and atropine. Results. In recipient's atrium among 37 pts we recognized NSR only in 15/37 pts.; in the remaining 22 pts. we found: sinus bradycardia - in 3, atrial flutter - in 3, low voltage AF - in 12 and no electrical activity - in 4 pts. Positive response to isometric exercise and atropine was observed in 12/15 pts. and 8 of them received A2A2T or A2A2T/D pacing system. We found much better sensing and pacing conditions in donor (A wave 2,1 mV, p. threshold 0,8 V) than in recipient atrium (1,1 mV and 1,4 V respectively)...

865/2523

Tytuł oryginału: Atrial pacing of transplanted heart.
Autorzy: Kutarski Andrzej, Zakliczyński Michał, Oleszczak Krzysztof, Wojarski Jacek, Foremny Jerzy, Kuśnierz Jacek, Jaworska Maria, Puszczewicz Dariusz, Łakomski Bogdan, Kalarus Zbigniew, Religa Zbigniew, Widomska-Czekajska Teresa, Zembala Marian
Źródło: Ann. Transplant. 2002: 7 (2) s.18-27, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Orthotopic heart transplantation (OHT) is most effective method for treatment of irreversible heart failure. Pateitns after OHT considered for permanent pacing consist still present a challenge for the implanting physician due to distorted atrial geometry and specific electrophysiological conditions of atrium. The aim of our study was to analyse the effectiveness of permanent atrial pacing in these patients. Patients and method. We implanted atrial lead in 37 SND pts., 2 months - 7 years after OHT, (3 pts with coexisting AV block received ventricular lead). Only straight BP screw in leads and manually formed stylets were used; we found satisfactor pacing/sensing conditions in 25 pts in RA appendage or anterior/lateral wall, in 10 pts - in CS ostium region and in 2 - in proximal part of CS. Results. Allimplantations were successful and no patient received WI pacing system. One dislodged lead required revision (1/37, 3 p.c.) but this was not related to endomyocardial biopsy. In 2 pts, due to nacceptable low RA potential and/or high PTh values atrial lead was implanted to CS for sensing/pacing of left atrium. The average acute value of A wave were 2,4 mV and chronic 2,2 mV; values of pacing threshold were 0,9 V and 1,6 V respectively. Only in 13/37 pts native A waves were recorded but with amplitude 0,6 mV. Wenckebach point was 120/min only in 2 pts., in borders 130-160 bpm in 15 pts. and exceeded 170 bpm. in remained 20 pts. Retrograde VA conduction was intact in 33/37 pts, but in 4 pts exceeded 260/min...

866/2523

Tytuł oryginału: Effect of L-arginine on oxygen consumption and haemodynamic function of rat's heart exposed to cold cardioplegic ischaemia and reperfusion.
Autorzy: Okoński Piotr, Szram Stefan Mirosław, Mussur Mirosław, Banach Maciej, Fila Michał, Bielasik Karol, Kędziora Józef, Zasłonka Janusz
Źródło: Ann. Transplant. 2002: 7 (2) s.28-31, il., bibliogr. 17 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: Ischaemia - reperfusion damage causes injury of all heart cells. Loss of endothelium function and biologically active substances secreted by it can be essential to development of the damage. Ischaemia and reperfusion decreases the release of nitric oxide, which influences postischaemic coronary flow and return of ventricular function. Administration of L-arginine to cardioplegic and reperfusion solution can improve protection of heart and cause the return of left ventricular function after hypothermic ischaemia through preservation of endothelial cell functions and increase of release of nitric oxide. Objective: How addition of L-arginine to cardioplegic solution influences oxygen consumption by myocardium and its postischaemic haemodynamic function. Methods: The research was conducted on isolated heart model of 56 rats, divided into seven equal groups. The hearts were prepared with modified Neely method and were perfused with the use of apparatus in accordance with modified Langendorf method. The research was carried out in the following order: initial perfusion of the non-working and working heart, perfusion with cardioplegic solution, cold cardioplegic arrest and reperfusion of the non-working and working heart. Results: During initial perfusion, oxygen consumption was comparable in all groups. During cardioplegic perfusion, oxygen consumption was reduced in every group. At the time of reperfusion of non-working heart model, consumption of oxygen was increased. During reperfusion of the working heart, the lowest oxygen consumption was noted in group P, the highest in group DI...

867/2523

Tytuł oryginału: Pituitary function test and endocrine status in patient with cirrhosis of the liver before and after hepatic transplantation.
Autorzy: Seehofer Daniel, Steinmueller Thomas, Graef Klaus-Juergen, Rayes Nada, Wiegand Wolfgang, Tullius Stefan G., Settmacher Utz, Neuhaus Peter
Źródło: Ann. Transplant. 2002: 7 (2) s.32-37, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: Hormonal alterations caused by chronic liver disease are well described. In contrast, the reversibility of peripheral hormonal changes after orthotopic liver transplantation (OLT) has only partially been analyzed since the establishment of OLT as treatment of chronic hepatic failure. In addition it is not finally verified if chronic liver failure and especially hepatic encephalopathy also affect ituitary function. We therefore performed extensive endocrine studies including a global pituitary stimulation test before and after liver transplantation. Methods: 22 patients with chronic alcoholic and non alcoholic liver failure were included in this prospective study. Basal hormone values (ACTH, cortisol, FSH, GH, IGF-I, LH, oestradiol, PRL, thyroid hormones and testosterone) were measured before and up to 5 years after OLT. Furthermore all patients underwent pituitary function tests with application of GRF, TRH, GnRH, and CRF before, 3 weeks and 3 months after OLT. Results: Estratiol, LH and FSH increased significantly in postmenopausal and only slightly in premenopausal female patients after OLT. Total testosterone revealed no marked changes and was normal before and up to five years after OLT in male patients. After stimulation with GnRH the LH response in females and the FSH response in males was significantly higher three months after OLT than pretransplant. LH response in males and FSH response in females was only slightly higher after OLT. Peripheral IGF-I was low and growth hormone was elevated in all patietns prior OLT...

868/2523

Tytuł oryginału: High intensity regimens with autologous hematopoietic stem cell transplantation as treatment of multiple myeloma.
Autorzy: Awedan Abdulnaser A.
Źródło: Ann. Transplant. 2002: 7 (2) s.38-43, il., tab., bibliogr. 57 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The treatment of multiple myeloma still remains under investigation. Conventional chemotherapy with currently used agents (i.e., Melphalan) effects complete remission in no more than 5 p.c. of patients. High dose chemotherapy followed by hematopoietic stem cells transplantation results in complete remission rates between 25 p.c. and 75 p.c. and a 3-year probability of event-free survival between 40 p.c. and 60 p.c. but is not curative since most patients relapse after 1.5 to 3 years. Therefore, it becomes the treatment of choice for multiple myeloma. The drugs used in high dose therapy include: high dose melphalan (200 mg/mý) as single agent., melphalan (140 mg/mý) and total body irradiation (TBI), busulfan and melphalan... etc. The use of the peripheral blood stem cell transplantation has allowed a reduction in the toxicity of high-dose regimens, but has not led to an increase in the overall response rate or survival. Hematopoietic stem cells from peripheral blood are preferred for transplantation because they restore hematopoiesis more rapidly than do bone marrow cells and the numbers of tumor cells are lower in peripheral blood than bone marrow cells. Peripheral blood stem cell transplantation was associated with significant reduction in the duration of aplasia and transfusion requirements. Several regimens have been proposed for stem cells mobilization including: High-dose cyclophosphamide and G or GM-CSF, G-CSF alone, and cyclophosphamide and etoposide with G-CSF... ect. Further attempts to improve the results of autotransplantation have included intensification with tandem translantation (double transplants) and reduction of tumor cells in stem cell infusion...

869/2523

Tytuł oryginału: First kidney transplantation from a non-heart-beating donor in the Czech Republic.
Autorzy: Treska Vladislav, Hasman Daniel, Reischig Tomas, Certik Bohuslav, Cechura Milos, Racek Jaroslav, Trefil Ladislav, Hes Ondrej
Źródło: Ann. Transplant. 2002: 7 (2) s.45-49, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: The long-lasting decline in the number of kidney donors with brain death in recent years is the reason for a quest for new sources of kidney donors in the Czech Republic. One possible source to increase the number of donor kidneys is the program of obtaining kidneys from non-herat-beating donors (NHBDs). Based on previous experience abroad, optimum hospital conditions, and, first of all, three years of experimental work it was possible to realise an NHBD program for the first time in the Czech Republic, in the Transplant Centre of the Plzen Hospital in early 2002. The first two kidneys were obtaiend from a donor with a devastating gunshot brain injury (maastricht criteria, Class III). After machine perfusion (RM3 renal perfusion system, Waters Medical) both kidneys were transplanted into two recipients with chronic renal insufficiency (chronic glomerulonephritis, nephrosclerosis). Both kidneys resumed their function immediately. For immunosuppression, basiliximab (Simulect, Novartis), rapamycin (Rapamune, Wyeth), and methylprednisolone (Urbason solubile forte, Hoechst) were used. To improve renal function, cyclosporine A (sandimmune Neoral, Novartis) was started later in the course, simultaneously reducing the dose of rapamycin. The postoperative course was uneventful and both patients could be discharged on the twenty-first day of hospitalisation for further care at home.

870/2523

Tytuł oryginału: Prolongation of rat kidney graft survival after inoculation of allogeneic spleen cells: the effect of various routes of cell transfer.
Autorzy: Oko Andrzej, Idasiak-Piechocka Ilona, Pawlaczyk Krzysztof, Wruk Michał, Pawliczak Elżbieta, Czekalski Stanisław
Źródło: Ann. Transplant. 2002: 7 (2) s.51-53, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

szczury

Streszczenie angielskie: Objectives: It has been clearly demonstrated that after donor-specific cell transfer the prolongation of allograft survival can be obtained, but the problem of how to inoculate cells remains unresolved. In this study, the effect of either protal venous (PV) or systemic intravenous (IV) inoculations of rat donor-specific spleen cells on subsequent renal graft survival was evaluated. Methods: LEW recipients received 10**6 of spleen cells from allogeneic Fishcer or syngeneic donors 30 days before kidney transplantation by either PV or IV routes. Animals from the control group obtained buffered saline by the same routes. Results: Fischer grafts in nonimmunized LEW recipients were rejected after 9.6 ń 1.3 days. In contrast, the immunization of the recipients by the pretreatment with donor spleen cells prolonged renal allograft survival significantly (p 0.002). However, no difference in the graft survival was observed between animals ioculated with cells either by PV or by IV routes. In each studied group, long-time graft survival ( 100 days) was achieved in one case. Conclusions: These observations suggest that the effect of the various routes of allogeneic cell inoculations on subsequent organ graft survival time depends on the interval between the cell transfer and organ transplantation. Intravenous route seems to be as effective as PV route when transplantation is performed several days after donor-specific cell transfusion.

871/2523

Tytuł oryginału: Inhibiton of glutathione-related enzymes augments LPS-mediated cytokine biosynthesis: involvement of an IkB/NF-kB-sensitive pathway in the alveolar epithelium.
Autorzy: Haddad John J., Safieh-Garabedian Bared, Saad Nayef E., Lauterbach Ryszard
Źródło: Int. Immunopharmacol. 2002: 2 (11) s.1567-1583, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 313,577

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The regulation of lipopolysaccharide (LPS)-mediated pro-inflammatory cytokine biosynthesis by reduction by reduction-oxidation (redox)-sensitive enzymes involved in maintaining intracellular glutathione homeostasis was onvestigated in fetal alveolar type II epithelial cells (fATII). Inhibition of glutathione-oxidized disulfide reductase, which recycles GSSG - 2GSH, by the action of 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) augmented LPS-depenfent secretion of interleukin (IL)-1á, IL-6 and tumor necrosis factor (TNF)-ŕ. BCNU incresed [GSSG] concentration at the expense of [GSH], thereby favoring oxidation equilibrium. Inhibition of ç-glutamylcysteine synthetase, the rate-limiting enzyme in the biosynthesis of GSH, by the action of L=buthionine-(S,R)-sulfoxime (BSO), potentiated LPS-induced IL-1á, IL-6 and TNF-ŕ production. Similar to BCNU, BSO depleted [GSH] and induced the accumulation of [GSSG]. BCNU and BSO reduced LPS-mediated phosphorylation of inhibitory-kB (IkB-ŕ), allowing its cytosolic accumulation. This effect was associated with the inhibition of the nuclear translocation of selective nuclear factor (NF)-kB subunits: NF-kB1 (p50), RelA (p65), RelB (p68) and c-Rel 9p75), but not NFkb2 (p52). BCNU and BSO reduced LPS-induced NF-kB activation as determined by the electrophoretic mobility shift DNA-binding assay. Analytical analysis of the effect of modulating the dynamic redox ratio [GSH]+[GSSG])[GSSG] revealed a novel role for GSSG as a disulfhydryl compound which an inhibitory effect on NF-kB activation. In is concluded that selective modulation...

872/2523

Tytuł oryginału: Bone turnover markers and cytokines in joint fluid. Analyses in 10 patients with loose hip prosthesis and 39 with coxarthrosis.
Autorzy: Sypniewska Grażyna, Lis Kinga, Biliński Piotr J.
Źródło: Acta Orthop. Scand. 2002: 73 (5) s.518-522, il., tab., bibliogr. [27 poz.]
Sygnatura GBL: 301,078

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We analyzed bone turnover markers (osteocalcin, bone ALP, á-crosslaps-CTX) and cytokines (IL-1, IL-8 and IL-10) in hip joint fluid in 10 patients before revision surgery and in 39 with idiopathic coxarthrosis. patients with loose imlants had lower concentrations of resorption marker than those with arthrosis (0.8 vs 1.3 ng/mL), but bone formation marker osteocalcin was reduced (4.2 vs 22.6 ng/mL). IL-8 and IL-10 levels were elevated in patients with implant failure (870 vs 340 pg/mL; 14,3 vs 4.0 pg/mL). We found a negative correlation between the bone resorption marker (CTX) and IL-10 in cases with prosthesis loosening and a positive correlation between IL-10 and time-to-revision. We conclude that enhanced local production of inflammatory cytokines leading to supressed bone formation is a part of the loosening process. The expression of anti-inflammatory mediators is not sufficient to counteract the imbalance in bone turnover.

873/2523

Tytuł oryginału: Changes of propofol concentration in cerebrospinal fluid during continuous infusion.
Autorzy: Dawidowicz Andrzej, Kalityński Rafał, Nestorowicz Andrzej, Fijałkowska Anna
Źródło: Anesth. Analg. 2002: 95 (5) s.1282-1284, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 301,042

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: We studied the changes in the propofol concentration in the cerebrospinal fluid (CSF) in 14 patients, undergoing elective intracranial procedures, who were anesthetized with propofol administered by target-controlled infusion. During anesthesia, fentanyl and cisatracurium were administered as required. After intubation of the trachea, the lungs of the patients were ventilated to normocapnia with an oxygen-air mixture (Fio2 = 0.33). Arterial blood and CSF samples (from an intraventricular drain) were collected between 90-180 min after the induction of anesthesia. Blood propofol concentrations were stable, between 5.0 ń 1.98 and 4.5 ń 1.7 ćg/mL (mean ń sd). There was a significant decrease in the CSF propofol concentration, from 52.2 ń 35.01 ng/mL at 90 min to 28.6 ń 21.9 ng/mL min (P 0.05). The CSF propofol concentration at 180 min (21.4 ń 14.9 ng/mL) was not significantly different from the concnetration at 150 min. Some possible reasons for this decrease after commencing continuous intraventricular drainage are discused.

874/2523

Tytuł oryginału: A phase III placebo-controlled study in advanced head and neck cancer using intratumoural cisplatin/epinephrine gel.
Autorzy: Werner J. A., Kehrl W., Płużańska A., Arndt O., Lavery K. M., Glaholm J., Dietz A., Dyckhoff G., Maune S., Stewart M. E., Orenberg E. K., Leavitt R. D.
Źródło: Br. J. Cancer 2002: 87 (9) s.938-944, tab., bibliogr. 24 poz.
Sygnatura GBL: 301,683

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Patient with recurrent or refractory head and neck squamous cell carcinoma received cisplatin/epinephrine injectable gel or placebo gel injected directly into the clinically dominant tumour. The double-blind phase III trial comprised of up to 6 weekly treatments over 8 weeks, 4 weekly evaluation visits, and then monthy follow-up; open-label dosing began as needed after three blinded treatments. Tumour response was defined as complete (100 p.c. regression) or partial (50-99 p.c. regression) sustained for ň28 day, and patient benefit as attainment of paliative or preventive goals prospectively selected by investigators and patients. With cisplatin/epinephrine gel, 25 p.c. (14 out of 57) of tumours responded (16 p.c. complete regression, 96 partial regression), vs 3 p.c. (one out of 35, complete regression) with placebo (P = 0.007). Patient benefit was positively associated with target tumour response in the blinded period among cisplatn/epinephrine gel recipients (P = 0.024): 43 p.c. (six out of 14) of responders benefited, vs 12 p.c. (five out of 43) of non-responders. the most frequent adverse event was pain during injection and the next most frequent was local cytotoxic effects consistent with the gel's mode of action. systemic adverse events typical of intravenous cisplatin were uncommon. Intratumoral therapy with cisplatin/epinephrine gel provided safe, well-tolerated, effective palliative treatment for patients with locally advanced head and neck squamous cell carcinoma, who lack other satisfactory treatment options.

875/2523

Tytuł oryginału: Antagonists of platelet fibrinogen receptor are less effective in carriers of P1a2 polymorphism of á3 integrin.
Autorzy: Różalski Marcin, Watała Cezary
Źródło: Eur. J. Pharmacol. 2002: 454 (1) s.1-8, il., tab., bibliogr. [41] poz.
Sygnatura GBL: 312,088

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The polymorphism Leu33 - Pro (platelet-specific antigen; P1a1/a2) of platelet GPIIIa is a potential risk factor for arterial thrombosis. However, its influence on platelet function remains controversial and little is known about its impact on platelet sensitivity to GPIIb-IIIa antagonists. Our objective was to ompare the effectiveness of various GPIIb-IIIa antagonists in P1a2(+) and P1a2(-) carriers. Platelet aggregation was monitored in healthy donors including P1a2(-) (N = 31) and P1a2(+) subjects (N = 27; 23 P1a1/a2, 4 P1a2/a2) using the impendance and turbidimetric aggregation tecniques. We evaluated the inhibition of ADP- and collagen-induced aggregation by disintegrins, kistrin and echistatin, and the low-molecular-weight blockers, GR144053F ((4-[-[4-(aminoiminomethyl]-1-piperazinyl]-1-piperidineacetic acid, hydrochloride trihydrate) and eptifibatide (N6-(aminoiminomethyl)-N2-(mercaptol-1-oxopropyl-l-lysylglycyl-l-ŕ-aspartyl-l-tryptophyl-l-propyl-l-cysteinamide, cyclic (1 - 60-disulfe). Kistrin (10-30 nmol/l) inhibited ADP- and collagen-induced aggregation stronger in P1a2(-) donors than in P1a2(+) donors; there was a significant difference between 50 p.c. inhibitory concentrations (IC50). The same tendency occurred with moderate concentrations of eptifibatide (40-100nmol/l) and also at low concnetrations of GR144053F (5-10 nmol/l) and high cncnetrations of echistatin (80-150 nmol/l), although in the case of the two latter inhibitors, the estimated IC50 values were not significantly different. In conclusion, GPIIb-IIIa blockers representing various clases are less...

876/2523

Tytuł oryginału: Inhibition and possible induction of rat CYP2D after short- and long-term treatment with antidepressants.
Autorzy: Daniel Władysława A., Haduch A., Wójcikowski J.
Źródło: J. Pharm. Pharmacol. 2002: 54 (11) s.1545-1552, il., tab., bibliogr. [45] poz.
Sygnatura GBL: 300,961

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of this study was to investigate the influence of tricyclic (imipramine, amitriptyline, clomipramine, despiramine), selective serotonin reuptake inhibitors (SSRIs: fluoxetine, sertraline) and novel antidepressant drugs (mitrazapine, nefazodone) on the activity of CYP2D, measured as a rate of ethylmorphine )-deethylation. the reaction was studied in control liver microsomes in the presence of the antidepressants, as well as in microsomes of rats treated intraperitoneally for one day or two weeks (twice a day) with pharmacological doses of the drugs (imipramine, amitriptyline, clomipramine, nefazodone 10 mg kg**-1 i.p.; desipramine, fluoxetine, sertaline 5 mg kg**-1 i.p.; mitrazapine 3 mg kg**-1 i.p.), in the absence of the antiderpressants in-vitro. Antidepressants decreased the activity of the rat CYP2D by competitive inhibition of the emzyme, the potency of their inhibitory effect being as follows: clomipramine(Ki = 14 ćm) sertraline ÷ fluoxetine (Ki = 17 and 16 ćm, respectively) imipramine ÷ amitripine (Ki = 26 and 25 ćm, respectively) desipramine (Ki = 44 ćm) nefazodone (Ki = 55 ćm) mirtazapine (Ki = 107 ćm). A oneday treatment with antidepressants caused a significant decrease in the CYP2D activity after imipramine, fluoxetine and sertraline. After prolonged administration of antidepressants, the decreased CYP2D activity produced by imipramine, fluoxetine and sertraline was still maintained. Moreover, amitriptyline and nefazodone significantly decreased, while mirtazapine increased the activity of the enzyme...

877/2523

Tytuł oryginału: The striatum as a target for anti-rigor effects of an antagonist of mGluR1, but not an agonist of group II metabotropic glutamate receptors.
Autorzy: Ossowska Krystyna, Konieczny Jolanta, Pilc Andrzej, Wolfarth Stanisław
Źródło: Brain Res. 2002: 950 (1/2) s.88-94, il., bibliogr. 54 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of the present study was to find out whether the metabotropic receptor 1 (mGluR1) and group II mGluRs, localized in the striatum, are involved in antiparkinonian-like effects in rats. Haloperidol (1 mg/kg ip) induced parkinsonian-like muscle rigidity measured as an increased resistance of a rat's hind foot to passive flexion and extension at the ankle joint. (RS)-1-aminoindian-1,5-dicarboxylic acid (AIDA; 0.5-15 ćg/0.5 ćl), a potent and selective mGluR1 antagonist, or (2R,4R)-4-aminopyrrolidine-2,4-dicarboxylate (2R,4R-APDC; 7.5-15 ćg/0.5 ćl), a selective group II agonist, was injected bilaterally into the striatum of haloperidol-treated animals. AIDS in doses of 7.5-15 ć/g/0.5 ćl diminished the haloperidol-induced muscle rigidity. In contrast, 2R,4R-APDC injections were ineffective. The present results may suggest that the blockade of striatal mGluR1, but not the stimulation of group II mGluRs, may ameliorate parkinsonian muscle rigidity.

878/2523

Tytuł oryginału: Calcium-induced long-term potentiation in horizontal connections of rat motor cortex.
Autorzy: Hess Grzegorz
Źródło: Brain Res. 2002: 952 (1) s.142-145, il., bibliogr. 16 poz.
Sygnatura GBL: 312,085

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: A transient (10 min) exposure of brain slices of young adult rats to elevated extracellular calcium (5 mM) resulted in a long-lasting potentiation of field potentials evoked in layer II/III and layer V horizontal connections of the primary motor cortex. This form of synaptic plasticity was blocked by D,L-2-amino-5-phosphonovalerate (APV, 100 ćM), an antagonist of NMDA receptors.

879/2523

Tytuł oryginału: A prospective comparison of antibiotic usage in pediatric surgical patients: the safety, advantage, and effectiveness of the surgical infection society guidelines versus a common practice.
Autorzy: Górecki Wojciech, Grochowska Elżbieta, Krysta Mirosław, Wojciechowski Piotr, Taczanowska Anna, Stanek Beata
Źródło: J. Pediatr. Surg. 2002: 37 (10) s.1430-1434, tab., bibliogr. 26 poz.
Sygnatura GBL: 305,145

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: The Surgical Infection Society (SIS) guidelines for antibiotic (Ab) usage minimally changed the surgical practice. The authors believed that clinical evidence of the safety, advantage, and effectiveness of SIS guidelines may improve inappropriate usage of Ab. Methods: A group of dedicated surgeons attempted to use Ab appropriately to SIS guidelines in their patients (group A). A comparison was made with a concurrent cohort of patients (group B), treated by surgeons who were nonsupporters of SIS guidelines. The trial was conducted in 378 children between. March 1999 and May 2000. The outcome measures were morbidity and mortality rates, Ab treatment duration, timing of administration, unjustified spectrum, and swith of Ab. Analysis of variance and Xý test were used in statistics. P value less than .05 was considered significant (s). results: Group A included 124 patients and Group B, 254. There were no intergroup differences in age, sex, and clen/contaminated/ratio of procedures. The mean duration of Ab treatment was 3.9 days in group A, versus 7.1 days in group B (s), with overall rate of appropriateness of 77 p.c. versus 36 p.c. (s) and no differences in mortality and morbidity rates. Conclusion: Perhaps these results may change Ab usage in pediatric surgery.

880/2523

Tytuł oryginału: Anthracyclines potentiate activity against murine leukemias L 1210 and P388 in vivo and in vitro.
Autorzy: Szmigielska-Kapłon Anna, Ciesielska Ewa, Szmigiero Leszek, Robak Tadusz
Źródło: Eur. J. Haematol. 2002: 68 (6) s.370-375, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 304,912

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Abstract: The interactions of 2-chlorodeoxyadenosine (2-CdA, cladribine) and three anthracyclines: doxorubicin (DOX), idarubicin (IDA) and mitoxantrone (MIT) were evaluated on murine leukemias P 388 adn L 1210. Prolongation of survival time of animals receiving drugs in combination compared to mice tereated with drugs in monotherapy was tested. We have also evaluated interactions of the cytostatics on murine leukemias in vitro by measuring their inhibitory effects on P 388 and L 1210 cell proliferation. We have observed a synergistic effect of MIT and IDA in combination with 2-CdA on P 388 leukemia resulting in an increase of life span (ILS) = 226 p.c in case of MIT + 2-CdA and ILS = 126 p.c. in the case of IDA + 2-CdA, whereas 2-CdA used as a sole drug resulted in an ILS = 47 p.c. The survival time of animals inoculated with P 388 leukemic cells and treated with DOX + 2-CdA was similar to ILS gained by DOX monotherapy (178 p.c. and 200 p.c. respectively). The mice bearing L 1210 leukemia receiving combined chamotherapy lived significantly longer than the animals on single agent regiments. The animals treated with schedule 2-CdA + MIT lived significantly longer (P = 0.004) as compard to the groups receiving drugs in monotherapy (ILS of 2-CdA + MIT group = 60 p.c., ILS of MIT groups 33 p.c., and 2-CdA group 33 p.c.). Finally, combination of DOX or IDA with 2-CdA resulted in ILS = 73 p.c. (2-CdA + DOX regimen), and ILS = 60 p.c. in case of 2-CdA + IDA regimen, which is significantly higher than ILS gained on monotherapy schedules. In vitro tests revealed that all tested anthracyclines enhance the antiproliferative activity of 2-CdA against L 1210 and P 388 leukemic cells (p 0.05)...

881/2523

Tytuł oryginału: Re-treatment with cladribine-based regimens in relapsed patients with B-cell chronic lymphocytic leukemia. Efficacy and toxicity in comparison with previous treatment.
Autorzy: Robak Tadeusz, Boński Jerzy Z., Kasznicki Marek, Góra-Tybor Joanna, Hellmann Andrzej, Konopka Lech, Dmoszyńska Anna, Dwilewicz-Trojaczek Jadwiga, Wołowiec Dariusz
Źródło: Eur. J. Haematol. 2002: 69 (1) s.27-36, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 304,912

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was to determine the effectiveness and toxicity of cladribine (2-CdA) used alone or in combination with prednisone (P) or cyclophosphamide and mitoxantrone in te-treatment of patients with progressive B-cell chronic lymphocytic leukemia (B-CLL). We analyzed treatment outcome in 40 patients who had responded to previous treatment with 2-Cd-A-based regimens. Criteria for re-treatment were the same as for the first treatment. The patients were retreated with the same agents if they responded to the first treatment and then relapsed with progressive disease not earlier than 3 months after achieving the first response. Eight patients received 2-CdA alone (0.12 mg kg-1 d-1) i.v. for 5 d, and 21 patients additionally were given P (30 mg m-2 d-1) orally, also for 5 d. Eleven patients received 2-CdA for 3 d combined with cyclophosphamide (650 mg m-2) i.v. and mitoxantrone (10 mg m-2) i.v. on day 1 (CMC regimen). The cycles were repeated usually at 4 wk intervals or longer if severe myelosuppression or infections occurred. The therapy was finished if complete remission (CR) was achieved or until maximum of sic courses. Overall response (IR) in re-treatment was obtained in 16 out of 40 (40 p.c.) patients (95 p.c. CI 16-64), including 62 p.c. after 2-CdA, 33 p.c. after 2-CdA + P and 36 p.c. after CMC. CR was obtained in four (10 p.c.) patients. Residual disease evaluated in the patients with CR by surface immunophenotyping had been demonstrated in 5 out of 16 (31 p.c.) patients after the first treatment and in one out of four (25 p.c.) patients after re-treatment. The median...

882/2523

Tytuł oryginału: Aminoacyl-tRNA synthetases and aminoacylation of tRNA in the nuucleus.
Autorzy: Mucha Piotr
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.1-10, il., bibliogr. 68 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: This review is focused on findings concerning the presence of translocation apparatus components (aminoacyl-tRNA synthetases, aminoacyl-tRNA, elongation factors) as well as translation itself in the nucleus. A nuclear role of these molecules is unknow. New findings suggest that well-accepted model of spatial segregation of transcription and translation in eukaryotic cell may be oversimplification. Nuclear coupling of both these processes show us how exciting and surprising may be the world of the living cell.

883/2523

Tytuł oryginału: GATA-1 binding to the ŕv promoter negatively regulates expression of the integrin ŕv subunit in human leukemic K562 cells.
Autorzy: Czyż Małgorzata, Stasiak Marta, Boncela Joanna, Cierniewski Czesław S.
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.19-28, il., bibliogr. 30 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions - 194 and - 172 of the ŕv promoter region and are directly involved in the regulation of transcriptional activity of teh ŕv gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates ŕv expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the ŕv gene promoter. DNase I footprinting analysis revealed that region - 413 to - 408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced ŕv mRNA and ŕv protein onn the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated wtih increased leveld of ŕv. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the ŕv gene promoter and negatively regulates ŕv gene expression.

884/2523

Tytuł oryginału: Selectivity of oxidative stress targeting in estorgen-induced experimental nephrocarcinogenesis.
Autorzy: Kobiela Jarek, Krajewski Jacek, Kalińska-Błach Beata, Stefaniak Tomasz
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.51-58, il., tab., bibliogr. 38 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

Streszczenie angielskie: Specificity of targeting of the oxidative stress towards lipid and protein fractions in a model of estrogen-induced Syrian hamster neprhocarcinogenesis was evaluated. The amount of proteins modified by oxidative stress was significantly elevated as early as one month after the initial implantation of estradiol to the experimental versus the control group, while the stress did not afect lipids. Subcellular localization of the oxidative stress target wass determined by the analysis of protein oxidation in subcellular fractions of kidney cells. The endoplasmic reticulum membranes were the fraction most affected bay the oxidative stress.

885/2523

Tytuł oryginału: Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells.
Autorzy: Gołębiewska Justyna, Rozwadowski Piotr, Spodnik Jan Henryk, Knap Narcyz, Wakabayashi Takashi, Woźniak Michał
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.59-65, il., bibliogr. 25 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We have demonstrated for the first time that the steroid metabolite, 2-methoxyestradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 ćM 2-ME inhibited cell cycle at G1 phase while 10 ćM 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol - the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell.

886/2523

Tytuł oryginału: Comparative in vitro studies on liposomal formulations of amphotericin B and its derivative, N-methyl-N-D-fructosyl amphotericin B methyl ester (MFAME).
Autorzy: Cybulska Barbara, Kupczyk Karolina, Szlinder-Richert Joanna, Borowski Edward
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.67-75, il., tab., bibliogr. 14 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: N-Methyl-N-D-fructosyl amphotericin B methyl ester (MFAME) is a semisynthetic derivative of the antifungal antibiotic amphotericin B (AMB). In contrast to the parent antibiotic, the derivative is charactiersed by low toxcity to mammalian cells and good solubility in water of its salts. Comparative studies on biological properties of free MFAME, AMB and their liposomal formulations were performed. To obtain liposomal forms, the antibiotics were inocporated into small unilamellar vesicles composed of dimyristoyl phosphatidylcholine (DMPC) and DMPC: cholesterol or ergosterol, 8:2 molar ratio. The effectivity of the liposomal and free forms of AMB and MFAME were compared by a determination of fungistatic and fungicidal activity against Candida albicans ATCC 10261, potassium release from erythrocytes, and haemolysis. The results obtained indicate that in contrast to AMB, incorporation of MFAME into liposomes did not further improve its selective toxicity. Studies on the antagonistic effect of ergosterol and cholesterol on the antifungal activity of the antibiotics indicated that sterol interference was definitely less pronounced in the case of MFAME than in the case of AMB.

887/2523

Tytuł oryginału: Facilitated diffusion of glucosamine-6-phosphate synthase inhibitors enhances their antifungal acitivity.
Autorzy: Janiak Agnieszka, Cybulska Barbara, Szlinder-Richert Joanna, Borowski Edward, Milewski Sławomir
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.77-86, il., tab., bibliogr. 23 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) and 2-amino-2-deoxy-D-glucitol-6-phosphate (ADGP) are strong inhibitors of the essential fungal enzyme, glucosamine-6-phospahte synthase, but their antifungal activity is poor, due to slow penetraton of these agents through the cytoplasmic membrane. In the present studies we have exploited the possibility of enhancement of ADGP and FMDP antifungal activity by improving their transport properties. It has been found that membrane-permeabilising polyene macrolides amphotericin B (AMB) and its N-methyl-N-fructosyl metyl ester derivative (MF-AME), at subinhibitory concentrations, facilitate diffusion of ADGP through the fungal cell membrane, thus allowing a decrease of its minimal inhibitory concentration (MIC). Synergistic effects have been observed for combinations of ADGP with AMB or MF-AME. Fractional inhibitory concentration (FIC) indexes, determined against a number of Candida spp., have been in the 0.18 - 0.81 range. Weak antifungal synergistic effects have been found for combinations of FMDP with AMB or MF-AME.

888/2523

Tytuł oryginału: The ability to overcome multidrug resistance of tumor cell lines by novel acridine cytostatics with condensed heterocyclic rings.
Autorzy: Bontemps-Gracz Maria M., Kupiec Agnieszka, Antonini Ippolito, Borowski Edward
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.87-92, il., tab., bibliogr. 9 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Two reacently syntehsized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our earlier hypothesis on the essential role of heterocyclic ring fused to the acridine moiety in the ability of acridine cytostatics to overcome multidrug resistance of tumor cells. The presence, location and kind of substituents considerably influenced both the cytotoxic acitivity of the derivatives and their ability to overcome multidrug resistance. The same factors also affected the cytostatics ability to differentiate between rumor cell lines with various types of drug exporting pumps.

889/2523

Tytuł oryginału: Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report.
Autorzy: Styczyński Jan, Wysocki Mariusz, Dębski Robert, Balwierz Walentyna, Rokicka-Milewska Roma, Matysiak Michał, Balcerska Anna, Kowalczyk Jerzy, Wachowiak Jacek, Sońta-Jakimczyk Danuta, Chybicka Alicja
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.93-98, il., tab., bibliogr. 17 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In vitro antileukemic activity of five glucocorticoids and their cross-resistance pattern in childhood acute lymphoblastic and non-lymphoblastic leukemia were determined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 ćM hydrocoritisone (HC), 8 ćM prednisolone (PRE), 1.5 ćM methylprednisolone (MPR), 0.44 ćM dexamethasone (DX) and 0.22 ćM betamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX ( 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). The AML cell samples were even more resistant to: PRE ( 85-fold, p = 0.001), DX ( 34-fold, p = 0.004), MPR ( 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A sigificant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.

890/2523

Tytuł oryginału: The influence of intracellular idarubicin and daunorubicin levels on drug cytotoxicity in childhood acute leukemia.
Autorzy: Styczyński Jan, Wysocki Mariusz, Dębski Robert, Kurylak Andrzej, Balwierz Walentyna, Rokicka-Milewska Roma, Matysiak Michał, Balcerska Anna, Kowalczyk Jerzy, Wachowiak Jacek, Sońta-Jakimczyk Danuta, Chybicka Alicja
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.99-107, il., tab., bibliogr. 14 poz. - 8 Międzynarodowe Sympzjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Uptake and efflux of two anthracyclines, idarubicin (IDA) and daunorubicin (DNR), was studied in childhood acute leukemia samples. A comparison of IDA and DNR transport phenomena in relation to drug cytotoxicity and expression of P-glycoprotein (PGP) was made. Intracellular content of IDA/DNR was determiend by flow cytomery using the fluorescent properties of the drugs. In vitro drug cytotoxicity was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. PGP expression was analysed by flow cytometry. The uptake and efflux rates were non-significantly higher for IDA than DNR. There were no differences between three types of leukemia with respect to drug content during accumulationand retention. After correction for the cell volume, intracellular concentration of both drugs in each moment of uptake and efflux was significantly lower in relapsed ALL and AML samples in comparison with initial ALL cells. Efflux, but not uptake, of both drugs was inversely correlated with PGP expression ad IDA, but not DNR, cytotoxicity. The cytotoxicity was correlated with drug accumulation for both drugs and with drug retention for IDA. In conclusion, it seems that (1) intracellular content was related to the lippopholic properties of the drugs rather than to the type of leukemia, (2) decreased intracellular concentration of both drugs might have an impact on compromised therapy results in AML and relapsed ALL children, (3) IDA presents higher cytotoxicity, which possibly might be decreased by the presence of PGP. These results might have a practical impact on the rational design of new chemotherapy protocols.

891/2523

Tytuł oryginału: Influence of G2 arrest on the cytotoxicity of DNA topoismerase inhibitors toward human carcinoma cells with different p53 status.
Autorzy: Bożko Przemysław, Larsen Annette K., Raymond Eric, Składanowski Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.109-119, il., bibliogr. 29 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-incubation in drug-free medium. RKO cells expressing wild-type, functional p53 arrested the cell cycle progression in both the G1 an G2 phases of the cell cycle whereas teh RKO/E6cells, which lack functional p53, only arrested in the G2 phase. Growth-arrested cells did not resuem proliferetion even after prolonged incubation in drug-free medium (up to 96 h). To evaluate the importance of the cell cycle arrest on cellular survival, a non-toxic dose of UCN-01 (100 nM) was added to the growth-arrested cells. The addition of UCN-01 was accompanied by mitotic entry as revealed by the appearance of condensed chromatin and the MPM-2 phosphoepitope, which is charactieristic for mitotic cells, G2 exit and mitotic transit was accompanied by a rapid activation of caspase-4 and apoptotic cell death. The influence of UCN-01 on the long-term cytotoxic effects of the two drugs was also determined. Unexpectedly, abrogation of the G2 arrest had no influence on the overall cytotoxicity of either drug. In contrast, addition of UCN-01 to cisplatin-treated RKO and RKO/E6 cells greatly increased the cytotoxic effects of the alkylating agent. These results strongly suggest that even prolonged cell cycle arrest in the G2 phase of the cell cycle is not necessarily coupled to efficient DNA repair and enhanced cellular survival as generally believed.

892/2523

Tytuł oryginału: TEL/JAK2 tyrosine kinase inhibits DNA repair in the presence of amifostine.
Autorzy: Gloc Ewa, Warszawski Mariusz, Młynarski Wojciech, Stolarska Małgorzata, Hoser Grażyna, Skorski Tomasz, Błasiak Janusz
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.121-128, il., bibliogr. 30 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The TEL/JAK2 chromosomal translocation (t(9;12)(p24;p13) is associated with T cell childhood acute lymphoblastic leukemia. The TEL/JAK2 fusion protein contains the JAK2 catalytic domain and the TEL-specific oligomerization domain. TEL-mediated oligomerization of the TEL/JAK2 proteins results in the constitutive activation of the tyrosine kinase activity. Leukemia cells expressing TEL/JAK2 tyrosine kinase become resistant to anti-neoplastic drugs. Amifostine is a pro-drug which can selectively protect normal tissues against the toxicity of anticancer drugs and radiation. We investigated the effects of amifostine on idarubicin-induced DNA damage and repair in murine pro-B lymphoid BaF3 cells and BaF3-TEL/JAK2-transformed cells using alkaline single cell gel electrophoresis (comet assay). Idarubicin induced DNA damage in both cell types but amifostine reduced its extent in control non-transformed BaF3 cells and enhanced it in TEL/JAK2-transformed cells. The transformed cells did not show measurable DNA repair after exposure to amifostine and idarubicin, but cells treated only with idarubicin were able to recover within a 60-min incubation. Because TEL/JAK2-transformed cells can be considered as model cells for certain human leukemias and lymphomas we anticipate an enhancement of idarubicin cytotoxicity by amifostine in these diseases. Moreover, TEL/JAK2 tyrosine kinase might be involved in cellular response to DNA damage. Amifostine could promote apoptosis or lower the threshold for apoptosis induction dependent on TEL/JAK2 activation.

893/2523

Tytuł oryginału: 5' - Esters of 2'-deoxyadenosine and 2-chloro-2'-deoxyadenosine with cell differentiation-provoking agents.
Autorzy: Grieb Paweł, Kryczka Tomasz, Wójtowicz Radosław, Kawiak Jerzy, Kazimierczuk Zygmunt
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.129-137, il., bibliogr. 20 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Phenylacetic and retinoic acids are carboxyacidic cell differentiating agents displaying anticancer activities. We report on a new class of compounds including the 5'-esters of 2'-deoxyadenosine (dA) or 2-chloro-2'-deoxyadenosine (cladribine, 2CdA) and the aforementioned acids. The rationale behind the synthesis of these esters was that if they are hydrolyzed inside the lymphoid cells, either dA will be removed from the intracellular environment by deamination, or 2CdA will be phosphorylated and accumulated. In either case targetted delivery of the differentiating agent to the lymphoid cells may be envisaged. The said compounds were synthesized by the Mitsunobu procedure employing triphenylphosphine and azadircarboxylic acid esters, and their stability was tested against various esterases. Esters of dA and 2CdA with phenylacetic acids were found to be resistant to enzymatic hydrolysis, whereas those with retinoic acids were efficiently hydrolyzed by commercially available hepatic esterase as well as by esterases present in the blood plasma and in diluted human lymphocyte lysate. Susceptibility to enzymatic hydrolysis was found to be a prerequisite of cytotoxic and/or differentiating activity of these esters in leukemic cell lines.

894/2523

Tytuł oryginału: Cytotoxic effect of nitric oxide on human hematological malignant cells.
Autorzy: Tsumori Michihiro, Tanaka Junko, Koshimura Kunio, Kawaguchi Mikiko, Murakami Yoshio, Kato Yuzuru
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.139-144, il., tab., bibliogr. 10 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.

895/2523

Tytuł oryginału: A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone.
Autorzy: Błasiak Janusz, Gloc Ewa, Warszawski Mariusz
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.145-155, il., bibliogr. 38 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Idarubicin is an anthracycline antibiotic used in cancer therapy. Mitoxantrone is an anthracycline analog with presumed better antineoplastic activity and lesser toxicity. Using the alkaline comet assay we showed that the drugs at 0.01 - 10 ćM induced DNA damage in normal human lymphocytes. The effect induced by idarubicin was more pronounced than by mitoxantrone (P 0.001). The cells treated with mitoxantrone at 1 ćM were able to repair damage to their DNA within a 30-min incubation, whereas the lymphocytes exposed to idarubicin needed 180 min. Since anthracyclines are known to produce free redicals, we checked whether reactive oxygen species might be involved in the observed DNA damage. Catalase, an enzyme inactivating hydrogen peroxide, decreased the extnt of DNA damage induced by idarubicin, but did not affect the extent evoked by mitoxantrone. Lymphocytes exposed to the drugs and treated with endonuclease III or formamidopyrimidine-DNA glycosylase (Fpg), enzymes recognizing and nicking oxidized bases, displayed a higher level of DNA damage than the untreated ones. 3-Methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing and nicking mainly methylated bases in DNA, increased the extent of DNA damage caused by idarubicin, but not that induced by mitoxantrone. Our results indicate that the induction of secondary malignancies should be taken into account as side effects of the two drugs. Direct strand breaks, oxidation and methylation of the DNA bases can underlie the DNA-damaging effect of idarubicin, whereas mitoxantrone can induces strand breaks and modification of the bases, including oxidation. The observed in normal lymphocytes much lesser genotoxicity of mitoxantrone compared to idarubicin should be taken into account in planning chemotherapeutic strategies.

896/2523

Tytuł oryginału: The analysis of structure - anticancer and antiviral activity relationships for macrocyclic pyridinophanes and their analogues on the basis of 4D QSAR models (simplex representation of molecular structure).
Autorzy: Kuz'min Victor E., Artemenko Anatoly G., Lozitsky Victor P., Muratov Eugene N., Fedtchouk Alla S., Dyachenko Natalia S., Nosach Lidiya N., Gridina Tatiyana L., Shitikova Larisa I., Mudrik Liubov M., Mescheriakov Aleksey K., Chelombitko Vladislav A., Zheltvay Andrey I., Eynde Jean-Jaques Vanden
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.157-168, il., tab., bibliogr. 19 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: A new 4D-QSAR approach has been considered. For all investigated moleucles the 3D structural models have been created and the set of conformers (fourth dimension) have been used. Each conformer is represented as a system of different simplexes (tetratomic fragments of fixed structure, chirality and symmetry). The investigation of influence of molecular structure of macrocyclic pyridinophanes, their analgues and certain other compounds on anticancer and antiviral (anti-influenza, antiherpes and antiadenovirus) activity has been carried out by means of the 4D-QSAR. Statistic charactierstics from QSAR of PLS (partial least squares) models are satisfactory (R = 0.94 - 0.97; CVR = 0.63 - 0.83).

897/2523

Tytuł oryginału: Isophosphoramide mustard analogues as prodrugs for anticancer gene-directed enzyme-prodrug therapy (GDEPT).
Autorzy: Misiura Konrad, Szymanowicz Daria, Kuśnierczyk Halina, Wietrzyk Joanna, Opolski Adam
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.169-176, il., tab., bibliogr. 18 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Two types of prodrugs, benzyl analogues of isophosphoramide mustard (iPAM), activated by cytochrome P450, and acylthioethyl analogues, activated by esterases, were designed. In contrast to iPAM that hydrolyse rapidly, the examined compounds are stable in phosphate-vuffered saline and Tris buffer. Benzyl analgoues of iPAM are poor substrates for cytochrome P450, are not cytotoxic and posses no antitumour activity. Acylthiethyl analogues of iPAM are good substrates for pig liver esterase, are cytotoxic and exertanti tumour activity against L1210 leukaemia in mice. The observed correlation for iPAM analogues between their susceptibility to hydrolysis and cytotoxicity and antitumour activity suggests possible application of these compounds as the prodrugs in gene-directed enzyme-prodrug therapy.

898/2523

Tytuł oryginału: New carbocyclic analogues of netropsin: Synthesis and inhibition of topoisomerases.
Autorzy: Pućkowska Anna, Bielawski Krzysztof, Bielawska Anna, Różański Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.177-183, il., bibliogr. 25 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: A series of carbocyclic analgues of netropsin were synthesized and evaluated for their capacity to inhibit human topoisomerases I and II in vitro. The compounds are oligopeptides containing 1,4-di- and 1,2,5-trisubstituted banzene rings and unsubstituted N-terminal NH2 groups. Compounds 4 - 7 consist of two netropsin-like units linked by aliphatic (tetra- and hexamthylene) chains. In the topoisomearse I and II assay, the relaxation of pBR322 plasmid was inhibited by compounds 4 - 7 at 100 ćM concentration.

899/2523

Tytuł oryginału: Synthesis, antiprotozoal and antibacterial activity of nitro- and halogeno-substituted benzimidazole derivatives.
Autorzy: Kazimierczuk Zygmunt, Upcroft Jacqueline A., Upcroft Peter, Górska Agata, Starościak Bohdan, Laudy Agnieszka
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.185-195, il., tab., bibliogr. 20 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Two series of benzimidazole derivatives were synthesised. The first one was based on 5,6-dinitrobenzimidazole, the second one comprises 2-thioalkyl- and thioaryl-substituted modified benzimidazoles. Antibacterial and antiprotozoal activity of the newly obtainned compounds was studied. Some thioalkyl derivatives showed remarkable activity against nosocomial strains of Stenotrophomonas malthophilia, and an activity comparable to that of metronidazole agains Gram-positive and Gram-negative bacteria. Of the tested compounds, 5,6-dichloro-2-(4-nitrobenzylthio)-benzimidazole sowed the most distinct antiprotozoal activity.

900/2523

Tytuł oryginału: In vitro releas of cytotoxic nucleoside analogs from lactide-caprolactone and lactide-glycolide copolymers.
Autorzy: Kryczka Tomasz, Bero Maciej, Kasperczyk Janusz, Dobrzyński Piotr, Marciniec Barbara, Popielarz-Brzezińska Maria, Grieb Paweł
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.205-210, il., tab., bibliogr. 19 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The aims of our study were to assess the release of cytotoxic nucleoside analgs 5-fluorouracil and 2-chloro-2'-deoxyadenosine from different lactide-glycolide or lactide-caprolactone biodegradable copolymers and the effects of sterilization on this release. The polymers were strilized either with ethylene oxide at 37řC, or with gamma radiation (15 kGy, 20 kGy, or 25 kBy). The kinetics of nucleoside release form the copolymers were measured over 50 days. Four copolymers exhibited realtively constant release of nucleosides in micromolar concentrations during the entire oberslightly influence nucleosides in micromolar concentrations during the entire observation period. Sterilization with either ethylene oxide or gamma radiation only slightly influence nucleoside release. Further development of these copolymers as an intracerebral nucleoside delivery system for local treatment of brain tumors is indicated.

901/2523

Tytuł oryginału: Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment.
Autorzy: Dzierżewicz Zofia, Orchel Arkadiusz, Węglarz Ludmiła, Latocha Małgorzata, Wilczok Tadeusz
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.211-220, il., bibliogr. 23 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Gut-derived adenocarcinoa Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on proliferation, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secretion. Differentiation was determined by brush border alkaline phosphoatase activity. Apoptosis was assessed by acridine orane and Hoechst stains. Differentiation and apoptosis were analyzed in both adherent and floating cell populations. The transformed Caco-2 cells did not retain their malignant phenotype in the presence of NaB. They appeared to undergo a change in the phenotype induced by NaB, as indicated by reduced proliferation, enhanced differentiation, stimulation of apoptosis leading to decreased viablility of cells, and stimulaiton of interleukin-8-secretion. Considering all the above facts and data, we postulate that Caco-2 cells cultured in NaB supplemented medium could regain the phenotypic characteirstics of the phenotype of the parent cell from which originated the caco-2 line.

902/2523

Tytuł oryginału: In vitro activity of oxazaphosphorines in childhood acute leukemia: Preliminary report.
Autorzy: Styczyński Jan, Wysocki Mariusz, Dębski Robert, Balwierz Walentyna, Rokicka-Milewska Roma, Matysiak Michał, Balcerska Anna, Kowalczyk Jerzy, Wachowiak Jacek, Sońta-Jakimczyk Danuta, Chybicka Alicja
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.221-225, il., tab., bibliogr. 9 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Glufosfamide (á-D-glucosyl-ifosfamide mustard) is a new agent for cancer chemotherapy. Its pharmacology is similar to commonly used oxazaphosphorines, but it does not require activation by hepatic cytochrome P-450 and preclinically demonstrates lower nephrotocity and myelosuppression than ifosfamide. The aim of the study was a comparison of the drug resistance profiles of gufosfamide and other exazaphosphorines in childhood acute leukemias. Leukemic cells, taken from children with ALL on diagnosis (n = 41), ALL on relapse (n = 12) and AML on diagnosis (n = 13) were analyzed by meand of the MTT assay. The following drugs were tested: glufosfamide (GLU), 4-HOO-ifosfamide (IFO), 4-HOO-cyclophosphamide (CYC) and malfosfamide cyclohexylamine salt (MAF). In the gorup of initial ALL samples median cytotoxicity values for GLU, IFO, CYC and MAF were 15.5, 33.8, 15.7 and 7.8 ćM, respectively. In comparison with initial ALL samples, the relative resistance for GLU and IFO in relapsed ALL samples was 1.9 (p = 0.049) and 1.3 (ns), and in initial AML samples 31 (p 0.001) and 5 (p = 0.001), respectively. All oxazaphosphorines presented highly significant cross-resistance. Glufosfamide presented high activity against lymphoblasts both on diagnosis and on relapse.

903/2523

Tytuł oryginału: Monitoring of urine nitric oxide (NO) related substrates and immunological competence in hematological malignancy.
Autorzy: Tanaka Junko, Koshimura Kunio, Tsumori Michihiro, Murakami Yoshio, Kato Yuzuru
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.227-232, il., tab., bibliogr. 10 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: It has been reported that concentrations of neopterin in the urine are changed according to the host immunological conditions. In the present study, we measured urinary concentration of neopterin in patients with malignant hematological disorders and investigated the relationship between urinary neopterin levels and laboratory indices for cellular immunity. Urine neopterin levels were correlated with serum sIL-2R levels in the patients with malignant lymphoma, and inversely correlated with lymphocyte reactivity with ConA in the patients with acute myeolocytic leukemia. However, no significant correlation was observed between uring neopterin levels and lymphocyte reactivity with phytohemagglutinin and pokeweed mitogen, CD4/8 ratio, CD56+16+ subset or serum IFN-ç levels. In the patients with malignant lymphoma, parallel changes in serum sIL-2R and uring neopterin were observed. The presented results suggest that urine neopterin levels are related to the activation of T cells in malignant lymphoma.

904/2523

Tytuł oryginału: Effect of cyclosporin A on immunological response in lungs of guinea pigs infected with Trichinella spiralis.
Autorzy: Dzik Jolanta M., Zieliński Zbigniew, Gołos Barbara, Jagielska Elżbieta, Wranicz Mariusz, Wałajtys-Rode Elżbieta
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.233-247, il., tab., bibliogr. s. 243-247 - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnki morskie

Streszczenie angielskie: The efects of cyclospirin A (CsA), a potent immunosuppressive drug with antiparasitic activity, on the innate immunological response in guinea pig lungs during an early period (6th adn 14th days) after T. spiralis infection were studied. CsA treatment of T. spiralis-infected guinea pigs caused a significant attenuation of immunological response in lungs by decreasing lymphocyte infiltration into pulmonary alveolar space, inhibiting alveolar macrophage superoxide anion production and lowering both the production of NO metabolites measured in bronchoalveolar lavage fluid and expression of the iNOS protein in lung homogenates, allowing us to speculate that the T. spiralis-dependent immunological reponse is dependent on lymphocyte T function. Interestingly, CsA itself had a pro-inflammatory effect, promoting leucotyte accumulation and macrophage superoxide production in guinea pig lungs. This observation may have a relevance to the situation in patients undergoing CsA therapy. Macrophage expression of the iNOS protein, evaluated by immunoblotting was not influenced by treatment of animals with CsA or anti-TGF-antibody, indicating different of the guinea pig and murine enzymes.

905/2523

Tytuł oryginału: In vivo effect of diallyl sulfide and cimetidine on phenacetin metabolism and biovailability in rat.
Autorzy: Szutowski Mirosław M., Zalewska Katarzyna, Jadczak Marta, Marek Monika
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.249-256, il., bibliogr. 29 poz. - 8 Międzynarodowe Sympozjum pt. Aspekty molekularne chemioterapii Gdańsk 09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Numerous cytochrome P450 inhibitors have been described as effective modulators of cytochrome P450 isoforms activity in vitro. Their inhibitory efficiency may be considerably modified after in vivo application. The aim of this study was to ecamine the effect of oral administration of diallyl sulfide - a cytochrome P450 2E1 inhibitor and cimetidien - a cytochrome P450 2C6 and 2C11 inhibitor on rat serum concentration of phenacetin and its metabolite acetaminophen. Both inhibitors increased area under the curve (AUC0-4h) for phenacetin by 50 p.c. Only cimetidine reduced AUC0-4h for acetaminophen indicating inhibition of O-deethylation activity. Quinidiene - a cytochrome P450 2D subfamily and P-glycoprotein inhibitor did not change significantly phenacetin biovailability. These results suggest that diallyl sufide inhibits the deacetylation pathway catalysed by arylamine N-acetyl transferase. Beside cytochroe P450 1A2 other cytochrome P450 isoforms (2A6 adn /or 2C11) are involved in phenacetin O-deethylation in rat.

906/2523

Tytuł oryginału: Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy.
Autorzy: Mroczek-Tońska Katarzyna, Ratajska Dorota, Guillot Cecile, Sąsiadek Maria, Ambroziak Anna, Lubos Leszek, Bartnik Ewa
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.257-262, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We have analysed the heteroplasmy level in 11 individuals from 3 families harbouring the mitochondrial 11778A mutation responsible for Leber hereditary optic neuropathy using last cycle hot PCR. The mutation level exceeded 90 p.c. both in affected and in unaffected individuals. We also checked whether any of the families belonged to the J haplogroup of mitochondrial DNA and obtained a negative results.

907/2523

Tytuł oryginału: Polymorphism in intron 23 of the endothelial nitric oxide synthase gene (NOS3) is not associated with hypertension.
Autorzy: Derebecka Natalia, Hołysz Marcin, Dankowski Rafał, Wierzchowiecki Michał, Trzeciak Wiesław H.
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.263-268, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Nitric oxide (NO) is synthesised in the vascular endothelium by nitric oxide synthase (NOS3) and is an important factor in the regulation of blood pressure. Imapired synthesis of NO due to mutations in the NOS3 gene is associated with hypertension. To date several allelic variants of the NOS3 gene have been identified and their possible linkage with hypertension investigated. We studied the distribution of genotypes and frequency of alleles of the G11T polymorphism in intron 23 of the NOS3 gene in patients with hypertension and in a control groups of healthy individuals. The polymorphism was determined by PCR-RFLP analysis. The distribution of genotypes in the patients with hypertension and in the healthy individuals did not differ significantly from the values predicted from Hardy-Weinberg equilibrium for the general population. No major differences in the distribution of the G11T polymorphsm in the patients and healthy individuals were found (P 0.05).

908/2523

Tytuł oryginału: Lymphocyte labile iron pool, plasma iron, transferrin saturation and ferritin levels in colon cancer patients.
Autorzy: Gackowski Daniel, Kruszewski Marcin, Banaszkiewicz Zbigniew, Jawień Arkadiusz, Oliński Ryszard
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.269-272, tab., bibliogr. 14 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Patients with colorectal carcinoma showed statistically significant lower values of transferrin saturation, total iron binding capacity and serum iron level as compared with control group, while the level of ferritin and the size of labile iron pool in carcinoma patients were higher, although this difference was not statistically significant. Our observations are in favour of the hypothesis which suggests that changes in iron metabolism restrict iron availability for tumour cells and as consequence, slow their growth.

909/2523

Tytuł oryginału: Adhesive properties of cacinoembryonic antigen glycoforms expressed in glycosylation-deficient Chinese hamster ovary cell lines.
Autorzy: Krop-Watorek Anna, Kłopocki Arkadiusz G., Czerwiński Marcin, Lisowska Elwira
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.273-283, il., bibliogr. s. 280-283
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

in vitro

Streszczenie angielskie: Carcinoembryonic antigen (CEA) is an oncofoetal cell surface glycoprotein that serves as an important tumour marker for colorectal and some other carcinomas. Its immunoglobulin-like structure places CEA within the immunoglobulin superfamily. CEA functions in several biological roles including homotypic and heterotypic (with other CEA familiy members) cell adhesion. Cell-cell interaction can be modulated by different factors, e.g., post-transplational modifications such as glycosylation. The purpose of this study was to examine whether changes in carbohydrate composition of CEA oligosaccharides can influence homotypic (CEA-CEA) interactions. In order to modulate glycosylation of CEA we used two different glycosylation mutants of Chinese hamster ovary (CH)) cells, Lec2 and Lec8. Lec2 cells showuld produce CEA with nonsialylated N-glycans, while Lec8 cells should yield more truncated sugar structures than Lec2. Parental CHO (Pro5) cells and the glycosylation deficient mutants were stably transfected wtih CEA cDNA. All three CEA glycoforms, tested in a solid-phase cell adhesion assay, showed an ability to mediate CEA-dependent cell adhesion, and no qualitative differences in the adhesion between the glycoforms were observed. Thus, it may be assumed that carbohydrates do not plas a role in homotypic adhesion, and the interactions between CEA molecules depend solely on the polypeptide structure.

910/2523

Tytuł oryginału: Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.
Autorzy: Zemlińska Barbara, Sochanik Aleksander, Missol-Kolka Ewa, Szala Stanisław
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.285-290, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Art-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.

911/2523

Tytuł oryginału: Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.
Autorzy: Kit Yuri, Kuligina Elena, Semenov Dimitry, Richter Vladimir
Źródło: Acta Bioch. Pol. 2002: 49 (1) s.291-294, il., biblogr. 15 poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-celluloses. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [ç-32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstratrated stimulation of the casein kinnase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.

912/2523

Tytuł oryginału: Gynecologic complications and management of HIV disease.
Autorzy: Nassiri R., Sikorski R[adzisław] J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.175-185, tab., bibliogr. 40 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Over 34.7 million people worldwide are estimated to be infected with HIV. In developing countries, new antiretroviral drug combiantions (HAART therapy) have reduced the incidence of AIDS and therefore, increased the life expectancy of those with the HIV disease. However, the number of women infected with HIV is increasing worldwide particularly, in lower socioeconomics in the third-world vountreis as weel as lower socioeconomics and minorities in the USA. The management of pregnancy in HIV-positive women should be seen as part of the comprehensive and longterm care of the woman provided to her. Obstetric management will be similar to that for uninfected women in most instances, although invasive diagnostic procedures should be avoided, and iron folate and other vitamin supplementation should be considered. In areas of high prevalence, these procedures should be for all pregnant women. HIV testing in pregnancy has a number of benefits in terms of prevention and care for mother and child but this must be balanced against possible risks of stigmatization, discrimination and violence. In order to avoid or minimize negative consequences, testing must be voluntary and confidential followed by quality counseling. Although the number of women infected with HIV is significatly lower in Poland than in other central and eastern European countries, the UNAIDS surveillance data suggest that the HIV cases may increase among women in Poland for the next decade. In this review, we delineate facts in gynecologic aspects of HIV infection and provide an up-to-date overview of clinical management of HIV infection in women.

913/2523

Tytuł oryginału: Genetic analysis of matrix metalloproteinase 3 (MMP-3) 5A/6A promoter polymorphism in women with ovarian cancer.
Autorzy: Szyłło K., Romanowicz-Makowska H., Niewiadomski M., Kozłowska E., Kulig A., Smolarz B[eata]
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.193-197, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: Matrix metalloproteinase 3 (MMP-3) overexpression is observed in cancer tissue. In the present work the relationship between genotypes of 5A/6A polymorphism in the promoter region of MMP-3 gene and the appearance of ovarian cancer was investigated. Patient and methods: Blood and tumour tissue were obtained from 20 women with ovarian cancer. Blood samples form age matched healthy women served as control. The genotypes of 5A/6A polymorphism were determined by PCR amplification using the allele specific primers. Results: There were significant differences in distributions of genotypes of 5A/6A polymorphism between patients and controls (p 0.05). No differences between genotypes of the 5A/6A polymorphism in blood and cancer tissue of each patient wre found. Conclusion: The results obtained indicated that the 5A/6A polymorphism of MMP-3 gene may be linked with appearance of ovarian cancer.

914/2523

Tytuł oryginału: Intracytoplasmic sperm injection in patients with idiopathic retrograde ejaculation.
Autorzy: Radwan M[ichał], Banaszczyk R., Pawlicki J., Kozarzewski M., Radwan J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.199-203, tab., bibliogr. 20 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Aim: The aim of the study was to estimate the effectiveness of the ICSI therapy of idiopathic retrograde ejeculation (RE) - induced infertility. Material and methods: In three couples in which the men suffered from idiopathic RE with 4.9 and 12 years of infertility, three ICSI cycles were performed with spermatozoa retrieved from the post ejaculatory urine. Results: The fertilization rate averaged higher than 60 p.c. Two clinical pregnancies were achieved. Conclusion: In case of idiopathic RE infertility, the causes cannot be established, ad thus it is difficult to find an effective therapy, the only reasonable solution for men with low sperm semen quality is to employ the ICSI procedure.

915/2523

Tytuł oryginału: Expression of nm23H1, proliferative activity and the presence of papillomavirus in squamous epithelial carcinoma of uterine cervix.
Autorzy: Kędzia W[itold], Schmidt M., Olejnik A., Goździcka-Józefiak A., Spaczyński M.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.205-211, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Purpose: the purpose of the paper was to analyse nm23H1 protein expression in squamous epithelial carcinoma of uterine cervix in relation to the stage and grade of carcinomas, clinical progression, proliferative activity and presence of HPV 16 and 18 viruses. Patients and methods: the expression of nm23H1 was analysed in 38 cases of squamous epithelial carcinma by means of the immunohistochemical method. For virological studies DNA was isolated from carcinomatous tissue and the presence of HPV 16 and 18 viruses determined by the PCR method. Results: no correlation was found between the accumulation of nm23H1 protein and the degree of clinical progression, histological maturity and presence of HPV 16 and 18 viruses. However, a statistically significant correlation was found between the group of not differentiated and well differentated carcinomas (p 0.0213). It was noted that all carcinomas characterised by overexpression of nm23H1 protein were HPV 16, 18 positive. Conclusions: overexpression of nm23H1 protein presence of HPV 16, 18 might indicate clinically more malignant form of squamous epithelial carcinoma of uterine cervix.

916/2523

Tytuł oryginału: N-acetyltransferase (NAT2) gene polymorphism in women with ovarian carcinoma.
Autorzy: Skorupska E[lżbieta], Friebe Z., Korzeniowska K.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.213-217, il., tab., bibliogr. 17 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Purpose: The aim of the study was a molecular analysis of N-acetyltransferase (NAT2) gene in the aspect of a risk group of ovarian carcinoma morbidity. Patients and methods: Fifty women with ovarian carcinoma (the study group) and fifty healthy women (the control group) were analysed. Analysis of a N-acetyltransferase gene was undertaken by PCR (polymerase chain reaction) - RFLP (restriction fragment length polymorphism). Results: This study shows that there is a statistically significant difference in the NAT2 alleles frequency distribution in the groups of ovarian carcinoma and healthy women. There is no statistically significant difference in the frequency distribution of NAT2 genotypes in those groups. Conclusions: Women with double mutant NAT2 alleles are genetically predisposed to the development of ovarian carcinoma. There is no association between particular NAT2 genotypes and morbidity of ovarian carcinoma.

917/2523

Tytuł oryginału: Pregnancy in a patient with hereditary angio-oedema (HANE) syndrome. A case report.
Autorzy: Smolarczyk R[oman], Grymowicz M., Czajkowski K., Broś M., Wójcicka-Bentyn J.
Źródło: Pol. J. Gynaecol. Investig. 2002: 4 (4) s.219-222, bibliogr. 9 poz.
Sygnatura GBL: 313,538

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Uncomplicated delivery in a 24-year-old woman with hereditary angio-oedema is presented in the study. Literature revieew about prognosis, treatment and prophylxis of HANE syndrome during pregnancy and labour has been included.

918/2523

Tytuł oryginału: Histone mRNA in situ hybridization in assessing proliferative activity of normal and malignant cells.
Autorzy: Słowiński Jerzy, Mazurek Urszula, Bierzyńska-Macyszyn Grażyna
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.335-339, il., tab., bibliogr. 48 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Proliferative capacity is an important determinant of tumour biological behaviour. For research and diagnostic purposes, immunohistochemical techniques are usually in the assessment of tissue proliferative status. An interesting alternative for these studies is a detection of histone mRNA. As the synthesis of histones is tightly coupled with DNA replication during S-phase of the cell cycle, histone mRNA level is a specific marker of S-phase cells. Furthermore, a short-lived transcript guarantees accurate estimation of S-phase cell pool at the moment of tissue fixation. The progress in molecular biology techniques during the last decade made possible the use of in situ hybridization, especially its non-radioactive version, in routine laboratory services. This technique can be successfully applied to detection of histone mRNA in routinely processed tissues. Advantages and limitations of such approach in tumour proliferation studies are discussed.

919/2523

Tytuł oryginału: IL-1-mediated inhibition of IL-6-induced STAT3 activation is modulated by IL-4, MAP kinase inhibitors and redox state of HepG2 cells.
Autorzy: Cisowski Jarosław, Zarębski Adrian, Koj Aleksander
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.341-345, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: It is known that during acute phase reaction IL-6 activates STAT3 in hepatoma cells and IL-1 downregulates this response. We found that the inhibitory properties of IL-1 on STAT signaling cascade in human hepatoma HepG2 cells are considerably decreased not only in the presence of MAP kinase inhibitors SB203580 and PD98059 but also by some antixidants (N-acetyl cysteine and pyrrolidine dithiocarbamate) and by anti-inflammatory cytokine IL-4. It appears that cytokien crosstalk between IL-6 and IL-1 includes a direct pathway sensitive to antioxidants and MAP kinase inhibitors, whereas the indirect prolonged response depends probably on synthsis of suppressors of cytokine signalling (SOCS).

920/2523

Tytuł oryginału: Expression of TCR-dżeta chain and apoptosis in subpopulations of tumor-associated lymphocytes (TALs) from malignant pleural effusions.
Autorzy: Sikora Jan, Żeromski Jan
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.347-351, il., bibliogr. 18 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Malignant effusions in serous carvities represent suspension of tumors and inflammatory cells and therefore present an attractive model for studying tumor-host interactions. T cell functional ability is linked to proper signal transduction via T cell receptor (TCR-CD3 complex), espceially its dżeta chain. The latter has been found to be often depressed in malignancy. Aim of this study was to assess alterations of dżeta chain expression and its relation to apoptosis of T lymphocytes. T lymphocytes obtained from pleural effusions of 20 cancer patients and 9 patients with nonmalignant effusions serving as a control were studied. The analysis was performed using flow cytometry combining CD3, CD4, CD8, CD16/56, TCR-dżeta and TUNEL reaction. In the control group, T lymphocytes obtained from pure lymphocyte effusions had constantly high level of dżeta chain while those from malignant pleural effusions had a decreased dżeta chain expression. Lymphocytes with low dżeta chain expression were the main subpopulation of T cells undergoing apoptosis.

921/2523

Tytuł oryginału: The compounds from the hollyhock extract (Althaea rosea Cav. var. nigra) affect the aromatization in rat testicular cells in vivo and in vitro.
Autorzy: Papież Monika, Gancarczyk Monika, Bilińska Barbara
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.353-359, il., bibliogr. 41 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Among medicinal plants, extract from the hollyhock flowers is a source of antocyanides and flavonoids. The latter coompounds belong, among others, to phytoestrogens (plant-derived dietary estrogens). The important role of estrogens in the testis is now well documented, and phytoestrogens, which may act as estrogen agonists or estrogen antagonists can also alter the reproductive function of the male. The aim of this study was to show whether the exposure of male rats to the aqueous hollyhock extract could affect the process of aromatization in their testes and in cultured Leydig cells. This was investigated by immunocytochemistry and radioimmunological assays. Immunoreactivities for aromatase and estrogen receptor á were weaker both in testicular sections and cultured Leydig cells after hollyhock extract administration when compared to the controls, while the intensity of immunoreaction for estrogen receptor ŕ remained unchanged. A lower level of estradiol secreted by cultured Leydig cells from the experimental group positively correlated with a direct inhibition of aromatase activity. Additionally, a quantitative analysis of flavonoid fraction from the hollyhock extract revealed the presence of quercetin and kaempherol. It seems that a weak antiestrogenic activity of flavonoid compounds present in the hollyhock extract is mediated through aromatase and estrogen receptor á rather than by estrogen receptor ŕ.

922/2523

Tytuł oryginału: Immunolocalization of PTHrP in prepubertal and pubertal testis of European bison.
Autorzy: Czykier Elżbieta, Zabel Maciej, Surdyk-Zasada Joanna
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.373-375, il., bibliogr. 23 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The present study deals with immunohistochemical localization of PTHrP in prepubertal and pubertal testis of European bison. PTHrP immunoreactivity was observed in germinal cells in the testis of both prepubertal and pubaertal animals. In calves, PTHrP was found in germinal cells, in seminiferous tubules lacking the lumen. The reaction was strong and regularly distributed within the cytoplasm. In adult animals, the reaction showed differentiation in spermatogenic cells. Some cells were strongly and diffusely stained, others exhibited weaker reaction of granular pattern. Sertoli cells and Leydig cells were PTHrP-negative in calves and adult animals.

923/2523

Tytuł oryginału: Immunoexpression of nm23 in advanced esophageal squamous cell carcinoma.
Autorzy: Szumiło Justyna, Skomra Danuta, Chibowski Daniel, Dąbrowski Andrzej, Wallner Grzegorz, Maciejewski Roman
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.377-380, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The study was undertaken to determine the immunoexpression of protein products of nm23 genes which are thought to be potential metastasis suppressor genes, in esophageal squamous cell carcinoma, and to analyze their relationship to selected clinicopathological features (age, sex, tumour size, depth of invasion, presence of lymph nodes and distant metastasis, pathologic tumor stage, degree of cancer differentiation and vascular/lymphatic ivasion), as well as to the overall survival. Immunohistochemical staining with monoclonal antibody against nm23 using LSAB2/HRP method on formalin-fixed, paraffin-embedded sections obtained from 32 tumors was performed. Eight tumors (25 p.c.) showed positive nm23 immunoexpression. There were no statistically significant differences between nm23-positive and nm23-negative groups with respect to all clinicopathological features studied. The positive nm23 status was related to a worse prognosis but this was not significant. The results may suggest that nm23-status is not associated with metastatic ability and prognosis in esophageal squamous cell carcinoma, but such thesis requires further study on a larger population.

924/2523

Tytuł oryginału: The effect of pre-eclamptic umbilical cord serum on fibroblast division in culture.
Autorzy: Pawlicka Elżbieta, Romanowicz Lech, Bańkowski Edward, Chyczewski Lech, Jaworski Stefan
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.381-384, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Edema, proteinuria, hypertension (EPH-gestosis), most commonly termed as pre-eclampsia, is the most common pregnancy-associated pathological syndrome. It is accompanied by a through remodelling of extracellular matrix in the umbilical cord tissues. It is commonly known that the presence of serum in culture medium storngly stimulates many functions of cells cultured in vitro. It was decided to check how the pre-eclamptic serum affects the fibroblast division in culture. Ki-67 is a protein present in proliferating cells and can be detected during all phases of the cell cycle (G1, S, G2/M) but not in resting (GO) cells. PCNA (proliferating cell nuclear antigen) is an intranuclear polypeptide whose synthesis rate is at its maximum during the S-phase of the cell cycle. The expression of Ki-67 and PCNA was measured by immunocytochemical methods and biosynthesis of DNA was evaluated by [14C]-thymidine incorporation. The activity of pre-eclamptic umbilical cord serum (UC-serum) was found to be distincly lower in comparison to control one. The expression of Ki and PCNA in fibroblast cultures treated wtih pre-eclaptic serum was also distinctly lower. Also the incorporation of [14C]-thymidine to DNA was lower than in the cultures treated with control UC-serum. It may by concluded that pre-eclampsia reduces the mitogenic activity of the umbilical cord serum.

925/2523

Tytuł oryginału: Iatrogenic Chlamydia infection-associated damage in the basilar arterial wall of the rat.
Autorzy: Chrapusta Stanisław J., Walski Michał
Źródło: Folia Histochem. Cytobiol. 2002: 40 (4) s.385-387, il., bibliogr. 8 poz.
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: A random finding of intracranial vascular Chlamydia sp. infection in Wistar rats and its associated damage of the component tissues of the basilar arterial wall are described as seen with transmission electron microscopy. The pathological changes observed showed no apparent specificity except for "striding" of the endothelial cells on the elastic lamina, the phenomenon observed in all five rats with basilar arterial Chlamydia infection, and in only two out of 13 rats with no proof of the infection. No atherosclerotic changes were found in the infected artery in rats sacrificed 8 or 26 weeks after the presumed infection.

926/2523

Tytuł oryginału: Depot-specific differences in the lipolytic effect of leptin on isolated white adipocytes.
Autorzy: Frhbeck Gema, Gomez-Ambrosi Javier
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR47-BR55, il., bibliogr. 40 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: In the present study we hypothesized that leptin-induced lipolytic effects show depot-specific differences. Material/Methods: Three-month-old male lean (+/+) and obese fa/fa rats were used. Lipolysis was determined in the absence or presence of leptin (0.63, 6.25 adn 62.5 nM) together with agents acting at the A1 adosine receptor of fat cells isolated from both subcutaneous and omental depots. The glycerol released into the incubation medium was taken as the lipolytic rate index. Results: The highest dose of leptin produced a 57.5 ń 7.3 p.c. and 70.3 ń 3.8 p.c. increase in omental and subcutaneous glycerol release, respectively, compared to the basal lipolytic rate (P 0.001). The addition of the three leptin concentrations in the ligand-free state produced a significantly greater stimulation of lipolysis in subcutaneous fat cells (P = 0.331; P = 0.0003 and P = 0.0015, respectively) compared to omental adipocytes. Under adenosine A1 receptor agonism and antagonism the same pattern of response was observed between visceral and subcutaneous adipocytes of lean rats. Althoug adenosine deaminase produced near maximum lipolysis in the adipocytes of lean animals, onl half of the maximum lipolytic rate (50.9 ń 3.2 p.c.) was achieved in fat cells from fa/fa rats (P = 0.0034). Leptin had no effect on the lipolytic activity of adipocytes from either localization in of/of rats. Conclusion: It can be concluded that decreeased sensitivity to the lipolytic effect of leptin in omental adipocytes compared to subcutaneous fat cells may underlie, at least in part, the association of visceral fat accumulation with increased co-morbidities.

927/2523

Tytuł oryginału: Hepatic cirrhosis increases sensitivity of kidney to endotoxin in rats.
Autorzy: Liu Jian-Jun, Wang Ji-Yao, Zhang Chi, Nilsson Ake, Duan Rui-Dong
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR56-BR60, il., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Background: Renal failure in cirrhotic patients is a severe complication and endotoxemia might be involved. We investigated the effect of endotoxin on renal function of cirrhotic rats and the potential protective role of N-acetylcysteine (NAC). Material/Methods: Hepatic cirrhosis was generated in a rat model by carbon tetrachloride. Both cirrhotic and normal rats were insulted by endotoxin intravenously, while another cirrhotic group was pretreated with NAC. Blood urea nitrogen (BUN) and creatinine were assayed eight hours later. The changes in serum tumor necrosis factor-ŕ (TNF-ŕ) were assayed by ELISA. The histological changes in the kidney were observed after hematoxylinand eosin staining. Results: Endotoxin increased the BUN and creatinine levels in both normal and cirrhotic rats, with a much higher elevation in the latter group. TNF-ŕ concentration was also increase by endotoxin; the changes are positively correlated with BUN and creatinine. NAC pretreatment sigificantly attenuates the effects of endotoxin on BUN, creatnine and TNF-ŕ levels in cirrhotic rats with no improvement in systemic toxicity symptoms. There were no obvious histological changes in the kidney of these animals. Conclusion: Hepatic cirrhosis increased the sensitivity of renal function to endotoxemia, which may be protected by NAC.

928/2523

Tytuł oryginału: A prolonged time interval between blood sample collection and centrifugation causes an increase in serum carbohydrate-deficient transferrin.
Autorzy: Arndt Torsten, Kropf Jrgen
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR61-BR64, il., tab., bibliogr. 3 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Carbohydrate-deficient transferrin (CDT) is used for the laboratory diagnosis of chronic alcohol abuse. Non-optimal preanalysis can cause an increase in CDT and false positive results. The aim of our study was to determine whether CDT results change over time between collection of the blood sample and centrifugation, and whether shipment of whole blood samples is a potential source of false positive CDT reports. Material/Methods: 152 blood samples were drawn from 38 persons (4 tubes per person, one venipuncture) and randomly assigned to 4 groups with different time intervals between blood sample collection and centrifugation (1h, 24h, 48h, 144h). CDT analysis was done using the ChronAlcol.D. assay. The statistical analysis was based on box-plots, ANOVA adn Kruskal-Wallis ANOVA. Results: The means and medians of CDT increased with the time of whole blood storage. ANOVA analysis of between-group differences was significant for mean CDT concentrations between 1 and 144 hours of whole blood storage. There was no correlation between CDT and free hemoglobin as a measure of hemolysis. An interference of hemolysis with CDT measurement can be excluded as the main cause of increased CDT results with whole blood storage time. Whether an in vitro degradation of the transferrin N-glycan chains causes the CDT increase should be evaluated by isoelectric foucusing of the transferrin isoforms in a further study. Conclusion: Storage or shipment of whole blood samples can shift initially normal CDT values to borderline and borderline to pathological CDT results.

929/2523

Tytuł oryginału: Antiapoptotic effect of (-)-deprenyl in rat kidney after ischemia-reperfusion.
Autorzy: Toronyi Eva, Hamar Janos, Magyar Kalman, Szende Bla
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR65-BR68, il., tab., bibliogr. 14 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Background: Since apoptosis of renal tubular cells is the damage caused by ischaemia-reperfusion, the antiapoptotic effect on kidney tubular epithelial cells of the monoamino oxidase-B (MAO-B) inhibitor (-) deprenyl (selegiline), known neuroprotective agent, with antiapoptotic properties, was studied in a rat model. Material/Methods: Warm renal ischaemia was caused by clamping the left renal artery of rats for 30 minutes. With the start of reperfusion 0.015 mg/kg, 0.15 mg/kg and 1.5 mg/kb of (-)-deprenyl was injected simultaneously into the carotid artery of the animals, respectively. Five rats severed as control, in which renal artery clamping was performed, but the rats were only treated with the solvent (physiological saline). After 6 hours of reperfusion the rats were exsanguinated and the kidneys were histologically examined. Results: Severe tubular damage characterised by apoptosis was found in the kidneys of the untreated rats. Apoptosis was verified on the basis of morphological features, methylgreen-pyronin staining and TUNEL reaction. (-)-Deprenyl diminished dose-dependetly the apoptotic damage, 0.15 mg/kg being the most effective dose. The same dose of(-)-Deprenyl is used in the therapy of human Parkinson's disease. Conclusion: Our findings suggest, that (-)-deprenyl might have an impact on decreasing renal injury also in cases of human cadaveric renal transplantation.

930/2523

Tytuł oryginału: Influence of losartan and enalapril on urinary excretion of 8-isoprostane in experimental nephrotic syndrome.
Autorzy: Tesar Vladimir, Zima Tomas, Jirsa jr Milan, Crkovska Jirina, Stipek Stanislav, Vernerova Zdena, Serakova Marketa
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR69-BR74, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The increased permeability of the glomerular capillary wall in adriamycin nephropathy may be mediated by incrased generation of free radicals, possibility also by the non-enzymatic production of isoprostanes induced by oxidative stress. ACE inhiitors and angiotensin II antagonists may reduce proteinuria, perhaps by decreasing intraglomerular pressure and increasing the selective permeability of the glomerular capillary wall. Material/Methods: We compared the effect of an ACE inhibitor, enalapril, and an angiotensin II atagonist, losartan, on total malodialdehyde in blood and the urinary excretion of certain eicosanodids and their metabolites (TxB2, 6-keto-PGF1a, bicyclo-PGE2 and 8-isoprostane) in experimental adriamycin-induced nephrotic syndrome in rats. Results: Increased proteinuria in adriamycin-treated rats was not prevented by losartan, but tended to be partly mitigated by enalapril. However, both losartan and enalapril prevented the adriamycin-induced increase of total MDA in serum, but urinary excretion of 8-isoprostane was increased in nephrotic rats treated by losartan compared to controls. The enalapril-induced increase in urinary excretion of bicyclo-PGE2 was possibly mediated by kinins. Proteiuria positively correlated with urinary excretion of 8-isoprostane, and proteinuric rats also had a significantly higher urinary excretion of 8-isoprostane than non-proteinuric rats. Conclusions: Proteinuria in the acute phase of adriamycine nephropathy may be dependent on free radical generation and the formation of 8-isoprostane. The mild anitproteinuric effect of enalapril, but not losartan, may suggest the contributory role of the inhibiton of kinin degradation in the antiproteinuric action of enalapril in this model of nephrotic syndrome.

931/2523

Tytuł oryginału: High affinity glutamate uptake in rat brain slices in experimental crush syndrome.
Autorzy: Knaryan Varduhi H., Arakelyan Ludmila N., Marouchyan Garegin L., Kevorkian Guevork A.
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR75-BR79, il., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Background: The pathogenesis of crush syndrome is severe traumatic damage to the organism accompanid by shock and stress, acte toxemia, etc. Glutamate has been shown to be implicated in excitotoxic neural death in various clinical settings. The high affinity glutamate uptake process plays a key role in normal synaptic transmission. The goal of our research was to determine the level of glutamate uptake in brain tissue slices after incorporation of [14C-U]glutamate. Material/Methods: An experimental model of crush syndrome in Wistar male rats was caused by 2 hours of compression of femoral soft tissues using a special press with a force of 100 kg/1 kg of animal weight. Slices were prepared and glutamate uptake was assayed immediately after compression, and again at 2, 24, and 48 hours of decompression. Results: The level of glutamate uptake in cerebral cortex and hypothalamus slices immediately after compression was reduced by 24 p.c. (p 0.05) adn 31 p.c. (p 0.005) respectively. Glutamate uptake in cerebral cortex and hypothalamus was nearly at the same level as the intact group of animals (without compression). In cerebellum slices, glutamate uptake also increased, and in fact exceeded the level in the intact group by 17 p.c. (p 0.025). The level of glutamate uptake decreased in cerebral cortex, hypothalamus, and cerebellum slices by 45 p.c. (p 0.001), 28 p.c. (p 0.001), and 25 p.c. (p 0.001) respectively, compard to the intact group of animals. Conclusion: Experimental crush syndrome promotes the reduction of the glutamate uptake level in brain tissue slices, such the cerebral cortex hypothalamus cerebellum.

932/2523

Tytuł oryginału: Minimal distances between temporal bone structures and their mutual correlations.
Autorzy: Wysocki Jarosław
Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR80-BR83, il., tab., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The minimal distances between basic temporal bone structures limit the operating field in ear surgery. The aim of the present study was to perform measurements on the minimal distances between key structures of the temporal bone and to investigate possible correlations between given structures. Material/Methods: The author remove 100 temporal bones from subjects of both sexes, across a range of age groups and from both sides of the body. Minimal distances were measured between the following structures: the lateral semicircular canal (SC), the tympanic ring (TR), the hard meninges of the middle cranial fossa (HM), the facial nerve (FN), the upper bulb of the internal jugular vein (JB) and the internal carotid artery (CA), collected in the appropriate pairs. The samples were measured using an operating microscope equipped with a graduated eyepiece. Results: The results are presented in various categories according to age, sex, and side of the body. Five pairs of distances were found to be significantly shorter in younger children (group I) than in older children and adults (group II). Conclusions: Ear surgery in younger children demands much more care than in older children and adults, especially regarding situations when the jugular bulb is in the operating field.

933/2523

Tytuł oryginału: mRNA expression of EGF receptor ligands in atrophic gastritis before and after Helicobacter pylori eradication.
Autorzy: Schiemann Uwe, Konturek Jan W., Assert Roland, Rembiasz Kazimierz, Konturek Stanisław J., Pfeiffer Andreas
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR53-CR58, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Epidermal growth factor (EGF) receptor ligands (EGFRL) including transforming growth factor alpha (TGF-ŕ), amphiregulin, and heparin binding -EGF (HB-EGF) are involved in gastric mucosal repair in chronic gastritis. Their mRNA expression has been shown to be upregulated after Helicobacter pylori (H. p.)-eradication but little is known about this gene expression in atrophic gastritis. The purpose of our study was to investigate EGFRL mRNA expression in gastric mucosa of patients with atrophic gastritis before and after H. p.-eradication. Material/Methods: Antral mucosal biopsies were obtained during endoscopy in 10 H. p. positive patients with atrophic gastritis and in 10 H. p. negative controls with intact mucosa. Total RNA of antral biopsies was extracted and RT-PCR was performed, the PCR-products being measured densitometrically. Values were compared with mRNA expressions in H. p. negative antral mucosa (n = 10). Results: Gastric biopsies revealed mRNA expression for TGF-ŕ, amphiregulin and HB-EGF, both in H. p. positive atrophic antritis and in H. p. negative healthy mucosa. The mRNA expression of TGF-ŕ in atrophic gastritis was significantly upregulated after H. p.-eradication, whereas that of amphiregulin did not change after this eradication. Expression of HB-EGF mRNA was higher in H.p.-infection than after H.p.-eradication or in H.p. negative healthy subjects. Conclusions: H.p. positive atrophic gastritis is associated with differential mRNA expression of EGF receptor ligands. H.p.-eradication in this entity leads to unequal changes of these growth factor expressions compared to chronic active gastritis without atrophy.

934/2523

Tytuł oryginału: Impact of moderate aerobic exercise training on insulin sensitivity in type 2 diabetic men treated with oral hypoglycemic agents: Is insulin sensitivity enhanced only in nonobese subjects?
Autorzy: Poirier Paul, Tremblay Angelo, Broderick Tom, Catellier Claude, Tancrede Gilles, Nadeau Andr
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR59-CR65, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Background: Few studies have evaluated the effect of aerobic physical training on insulin sensitivity in subjects with type 2 diabetes treated with oral hypoglycemic agents. Most studies that invollved nonobese subjects showed enhanced insulin sensiivity whereas studies involving obese subjects with type 2 dibetes are inconsistent because of concomitant fat loss. Material/Methods: Thirteen men with type 2 diabetes (2 nonobese and 7 obese) treated wtih oral hypoglycemic agents carried out a 12-week ergocycle program at 60 p.c. VO2 peak, one hour thrice a week. Diet and medications were maintained throughout the study. Insulin sensitivity was assessed with the euglycemic hyperinsulinemic clamp technique. Results: VO2 peak (ml/kg/min) increased with exercise training. There were no significant changes in fat mass, percent body fat, fasting plasma glucose and insulin levels, glycated hemoglobin levels, or insulin sensitivity. However, when the subjects were separated into two groups, they showed different insulin sensitivity adaptation to training. In fact, nonobese subjects with type 2 diabetes showed an increase in insulin sensitivity, whereas no change was observed in the obese subjects. Conclusions: These findings suggest that moderate aerobic exercise training of 3 months duration enhanced insulin sensitivity only in nonobese men with type 2 diabetes. Obese men with type 2 diabetes showed no benefit. Thus, adiposity per se may be a determining factor in the exercise-induced metabolic benefit of exercise training in subjects with type 2 diabetes.

935/2523

Tytuł oryginału: Application of perfluorocarbon liquid in the removal of metallic intraretinal foreign bodies.
Autorzy: Vartanyan Armen H., Hovhannisyan Tadevos A.
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR66-CR71, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: Virectomy is recognized as one of the best effective methods for removing interetinal foreign bodys, but many authors showed complications during or after removing IOFB. The purpose of our study was to show that the use of perfluorocarbon liquid minimizes complications during pars plana vitrectomy for the removal of metallic intraretinal foreign bodies (IRFBs). Material/Methods: Fourteeen consecutive eyes presenting with penetrating eye injury and intraretinal foreign bodies had previously undergone vitrectomy with injection of perfluorocarbon liquids and removal of IRFBs. In all cases a silicon oil or gas C3F8 tamponade was performed. In one case the removal of the foreign body was combined with repair of retinal detachment using internal tamponade with silicon oil. Results: After a mean follow-up of 16.4 months, 78.5 p.c. of the eyes had recovered better than 20/70 vision, and 92.8 p.c. had attached retina. One patient had endophthalmits and phthisis, and perioperative complications were encountered in two cases. In one case there was a limited hemorrhage, intraretinal, while the other complication was a retinal detachment, which was immediately correctd. In the follow-up period endophthalmitis and lens opcity was found in one patient and epiretinal membrane in another case. These postoperative complications were repaired by additional surgery. Conclusions: The usage of perfluorocarbon liquid is effective for removal of intraretinal foreign bodies and allows to minimize intraopertive complications.

936/2523

Tytuł oryginału: Does schistosomiasis interfere with application of the Knodell score for assessment of chronic hepatitis C?
Autorzy: Shiha Gamal, Zalata Khaled R.
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR72-CR77, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Schistosoma mansoni (SM) is a significant cause of liver disease in many countries. Chronic hepatitis C (HCV) is a worldwide health problem. The association of SM and chronic HCV is not uncommon, especially in areas where both diseases occur. The possible synergistic relationship between them is controversial. Also, the degree of necroinflammatory injury and the state of fibrosis in patients with mixed schistosomias and chronic HCV remains unclear. Material/Methods: 185 individuals were studied: 25 with pure SM, 100 with pure HCV, and 60 mixed HCV and SM (HCV + S), selected from 222 biopsied patients with chronic liver disease treated at the liver unit of the Internal Medicine Department, Mansoura University (July 1999 - May 2000). They were subjected to rectal snip and serological test for schistosomiasis, liver functions, HBV, HCV serological markers, serum qualitative PCR, and liver biopsy. Masson trichrome stain was performed to assess fibrosis. Immuohistochemical staining was performed for HBsAg and HBcAg. The Modified Knodell score was applied to assess the biopsy.

937/2523

Tytuł oryginału: Early cardiovascular involvement in patients with systemic sclerosis (SSc).
Autorzy: Wranicz Jerzy Krzysztof, Zielińska Marzenna, Cygankiewicz Iwona, Dziankowska-Bartkowiak Bożena, Sysa-Jędrzejewska Anna
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR78-CR82, tab., bibliogr. 28 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Bacgground. Systemic sclerosis (SSc) is a connective tissue disease characterized by progressive fibrosis of the skin, and by distinctive forms of internal organ involvement. Symptoms usually occur relatively late and are nonspecific, thus often unrecognized. The purpose of our study was to evaluate cardiac function in patients with SSc by means of non-invasive methods in order to detect early dysfunction of the cardiovascular system. Material/Methods: A group of 22 patients with SSc was compared with a group of 22 healthy volunteers. Standard EKG, 24-hour Holter monitoring (HM), and echocardiography were performed in all subjects. Results: In patients with SSc, HM revealed a tendency to tachycardia. Conduction disturbances were observed in 3 patients. In 6 patients significant ventricular arrhythmia was found. Silent ischemia episodes were detected in 6 patients. In HRV analyzis, significantly lower values were detected in patients with SSc than in controls. LPs were present in one patient with SSc, and none in the control gorup. The mean values for QT interval did not exceed the range of normal values. No signs of systolic cardial dysfunction were detected, while in 6 patients left ventricle diastolic dysfunction was recognized. Valvular lesions were observed in 8 patients, but only in 2 patients wer ethey hemodynamically important. Conclusions: 24-hour Holter minitoring and echocardiography enable the early detection of cardiovascukar dysfunction in patients with systemic sclerosis presenting without apparent cardiac impairment symptoms.

938/2523

Tytuł oryginału: Clinical signs pointing to the source of hemorrhage in multiple intracranial aneurysms.
Autorzy: Zderkiewicz Edward, Pawlik Zbigniew, Czochra Marian, Tabora Arkadiusz
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR83-CR86, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: In multiple aneurysms of cerebral vessels it is essential to correctly identify the aneurysm that has caused the hemorrhage, since it must be operated first. On the basis of our own material, we have evaluated the usefulness of various clinical signs and examinations in diagnosing which of several aneurysms was the cause of an intracranial hemorrhage. Material/Methods: The material consited of 163 patients with a total of 391 aneurysms. Diagnosis was based on panangiography and neurological examiantions in all cases and CT in the majority. Results.: All the diagnostic examiantions in question (neurological, angiography, CT and intraoperative evaluation) anabled a correct diagnosis in 72.6 p.c. of cases. In CT scanning, diagnostic value diministhes with time. Intracerebral hematoma is the most persistent sign. The highest rupture index a involved aneurysms localized on the anterior communicating artery, while those on the internal carotid artery ranked second. Four patients in whom intraoperative evaluation showed that the order in which the aneurysms wre treated srugically was erroeus died as the result of rebleeding that occurred prior to planned follow-up surgery. Conclusions: It is vitally important for outcome to determine which of several aneurysms has ruptured, since errors in the sequencing of surgical repair significantly increase mortality due to rebleeding preceding the next stage of surgery. When diagnosing the source of a hemorrhage one should take into account neurological findings, angiography, ECG, CT, MRI, and the location of the aneurysms.

939/2523

Tytuł oryginału: Is there a relationship between left ventricular systolic function and serum cytokines level in patients with coronary artery disease?
Autorzy: Mizia-Stec Katarzyna, Mandecki Tadeusz, Zahorska-Markiewicz Barbara, Janowska Joanna, Szulc Andrzej, Jastrzębska-Maj Ewa, Szymański Leszek
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR87-CR92, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: There is growing evidence that cytokines play a pathogenic role in both heart failure and atherosclerosis. The aim of our study was to assess serum levels of selected cytokines and soluble forms of receptors in patients with coronary artery disase (CAD), and the relation between these concentrations and left ventribular (LV) systolic function. Material/Metods: We measured serum levels of TNFŕ, sTNFR 1 and 2, IL-2, and IL-10 in 100 patients with CAD: 70 patients with LV regional wall motion disturbances (group A); and 30 patients with normal LV function (group B). The control group (group C) consisted of 20 healthy volunteers. Results: Mean serum TNFŕ and IL-10 concentrations were significantly higher in groups A (18.3 ń 3.5; 55.4 ń 118.5 pg/ml) and B (17.9 ń 4.9; 45.3 ń 76.8 pg/ml) than in controls (8.3 ń 1.4, p 0.001; 14.3 ń 28.5; p 0.05; respectively). Moreover, in group A serum levels of sTNFR 1 were higher (1367.4 ń 531.1 pg/ml) than in group C (1093,9 ń 456.9 pg/ml; p 0.05). No differences were found in the study groups between serum sTNFR 2 and IL-2 levels. In group A, both the LV ejection fraction and motion score index correlated with TNFŕ (r = -0.277; r = 0.282, p 0.05), and sTNFR 1 levels (r = -0.258; r = 0.280; p 0.05). Conclusion: The serum concentrations of TNFŕ and IL-10 are increased in patients with CAD. Additionally, patients with impaired LV contractility have higher sTNFR 1 concentrations. Serum cytokine activation may play a role in the development of heart failure in patients with CAD.

940/2523

Tytuł oryginału: Modified neck dissections - efficiency of surgical treatment and clinical observations.
Autorzy: Chodynicki Stanisław, Łazarczyk Bogdan, Woińska-Rojecka Teresa, Południewska Barbara, Łuczaj Jarosław, Tynkiewicz Jerzy, Olszewska Ewa
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR93-CR95, il., bibliogr. 5 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The evaluation of affected lymph node number, the site of metastatic lymph nodes, and spread of neoplastic infiltration beyond the lymph capsule is considered useful in prognosis. The aim of the study was to estimate the frequency of neck nodal metastases occurrence depending on the site of origin, the grade of larynx cancer progresssion, and clinical condition of neck lymph nodes. Material/Methods: The study comprised 315 patients with larynx cancer who underwent surgery in 1994-1999. Laryngectomy was the most frequent procedure, performed in 254 patients, while partial laryngectomy was performed in 61 patients. There were 630 bilateral neck dissections; type I of modified radical surgery in 27 cases, type II in 45 cases, and type III in 558 cases. Results: Neck nodal metastases were detected most rarely in cases of glotic cancer (16 p.c.), and most frequently in transglottic cancer (56 p.c.). Most frequently, the nodal metastases affected the II level of the lymph node groups regardless of the site. Recurrences to the neck lymph nodes were observed in 3.3 p.c. of the cases where the removed lymph nodes did not reveal any metastatic changes in histopathological examiantions. Conclusion: Postopeartive results of the neck lymph node histopathological examination point to the necessity of lymphadenectomy in the II-V levels. Selective lymphadenectomy is not advisable due to the possibility of metastases to all lvels of the neck lymph nodes regardles of the primary tumor site.

941/2523

Tytuł oryginału: Vitamin C concentration in gastric juice in patients with precancerous lesions of the stomach and gastric cancer.
Autorzy: Dąbrowska-Ufniarz Elżbieta, Dzieniszewski Jan, Jarosz Mirosław, Wartanowicz Maria
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR96-CR103, il., tab., bibliogr. 66 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The aim of the study was to elucidate what are the concentrations of vitamin C in gastric juice in patients with gastric cancer in comparison to patients with metaplasia. Material/Methods: In patients age 20 to 75 years with H. pylori infection and chronic gastritis, metaplasia and gastric cancer the concentration of vitamin C was determined by specrofotometry of gastric juice during gastroscopy. On the basis of the results of histological examination the following four groups were isolated. Group I (control) - 12 patients with normal gastric mucosa, group II - 16 patients with chronic gastritis, group III - 17 patients with metaplasia and group IV - 16 patients with gastric cancer H. pylori infection was confirmed by urease test and histological examination (Giemsa stainning) in all patients. Results: In controls the mean concentration of vitamin C in gastric juice was 18.2 ćg/ml (5.7 - 31.2 ćg/ml), in gorup II - 6.3 ćg/ml (2.9 - 13.1 ćg/ml) in group III - 3.9 ćg/ml (2.6 - 10.1 ćg/ml) and group IV - 3.2 ćg/ml (1.7 - 9.2 ćg/ml). Statistically significant differences of vitamin C concentration were found among group I and group II, III and IV (p 0.001) and among groups II and III and IV (r 0.01). Conclusion: There are not differences of vitamin C concentration in gastric juice between patients with metaplasia and patients with gastric cancer. This points out that low levele of this vitamin in gastirc juice might play the role in the earlier stages of carcinogenesis.

942/2523

Tytuł oryginału: Cytomegalovirus infection and atherogenesis.
Autorzy: Szkaradkiewicz Andrzej, Bogaczyńska-Staniewicz Joanna, Torliński Lech, Wal Marzena
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR104-CR107, tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: Background: Experimental studies and clinical observations indicate the importance of active CMV infection in the development of atherosclerosis. Our research constituted an attempt to evaluate the components of lipid metabolism (total cholesterol and its fractions, triglycerides) in children with active CMV infection. Material/Methods: The research involved 45 children with active primary or secondary CMV infection. The diagnosis was established by measuring anti-CMV serum antibodies (IgM and IgG) using the Enzyme-Linked Fluorescent Assay (ELFA) technique, and by detecting CMV-DNA using the PCR technique. On the basis of diagnostic tests, the children were allocated to one of two groups. The first group of 15 children manifested primary active infection with CMV. The other group consisted of 30 children diagnosed with secondary active CMV infection. Results: In the first group, the mean values of the serum lipid profile were as follows: total cholesterol - 142 ń 32.9 mg/dl; HDL-cholesterol: - 23.4 ń 8.6 mg/dl; LDL- cholesterol - 97.8 ń 30.9 mg/dl; triglycerides - 83 ń 15.5 mg/dl. In the second group of children, the mean values of serum lipid profile were as follows: total cholestserol - 142.5 ń 44.2 mg/dl; HDL- cholesterol - 21.4 ń 10.8 mg/dl, LDL-cholesterol - 87.1 ń 35.3 mg/dl; triglycerides - 101.7 ń 31.2 mg/dl. In all these children, the values of total cholesterol, LDL-cholesterol, and triglycerides conformed to published norms, while HDL-cholesterol was significantly belowe normal. Conclusion: The results we obtained point to dyslipidemia in the couse of active CMV infection, which may be of pathognetic significance at any stage of atherogenesis.

943/2523

Tytuł oryginału: Serum levels of tissue inhibitor of metalloproteinases 2 in systemic sclerosis: a preliminary study.
Autorzy: Dziankowska-Bartkowiak Bożena, Waszczykowska Elżbieta, Łuczyńska Maria, Zalewska Anna, Sysa-Jędrzejowska Anna
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR108-CR112, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: The etiopathogensis of systemic sclerosis (SSc) - an autoimmunological disease characterized by excess collagen and other connectve tissue components in skin and internal organs - remains unclear. Given the potential role of matrix enzymes, metalloproteinases, and their tissue inhibitors in pulmonary fibrosis, we assayed the serum concentration of tissue inhibitor of metalloproteinases 2 (TIMP-2) in patients with SSc and explored its possible correlation with the duration of Raynaud's phenomenon, the intensity of sclerosis of the skin, and pulmonary lesions. Material/Methods: We studied 18 SSc patients, 8 with limited SSc and ten with diffuse. The degree of sclerosis of the skin was measured using teh Total Skin Scale. Chest x-rays and spirometric examinations were also performed. The presence and type of antinuclear antibodies (ANA) were determined by indirect immunofluorescence and double immunodiffusion in agar gelatin. Serum TIMP-2 concetration was measured by ELISA. Results: The average serum TIMP-2 concentration was not significantly higher in SSc patients than in controls. No statistically significant difference was found in this respect between limited and diffuse SSc. Despite the lack of correlation between the duration of Raynaud's phenomenon, the degree of sclerosis of the skin, and the serum concentration of TIMP-2, the latter concentration was higher in patients with restrictive pulmonary dysfunction in spirometric testing. Conclusion: Our research, though involving a small group of patients, points to the probable role of TIMP-2 in the development of pulmonary fibrosis.

944/2523

Tytuł oryginału: Total IgE levels in children under three years of age.
Autorzy: Malinowska Elżbieta, Kaczmarski Maciej
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR113-CR118, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The course of type 1 allergic reaction is a result of complex immune processes in which immunoglobulin E plays a crucial role. The serum level of this immunoglobulin (IgE total) is closely related to the age of the patient. The presented paper analyses IGE total in children below 3. Material/Methods: The study was carried out in group of 291 patients aged 5 - 39 months. IgE total were determined using the Fluoro-FAST 3M. The analysis took into consideration the age, sex and living place of the examined children. Results: The artmetic mean of IgE total was 38.8 ń 4.0 IU/ml, the median - 12 IU/ml, whereas the 5th and 95th percentile values amounted to IIU/ml and 185 IU/ml, respectively. According to the standards recommended by the manufacturer of reagents used for determination of IgE total (Bio Whittaker), 103 children (35.4 p.c.) had elevated serum levels of IgE total, whereas 196 (67.4 p.c.) were found to have elevated IgE total according to population standards. The obtained percentage distribution of elevated IgE total values in particular age groups is characterized by stepwise changes, observed in the age intervals for which the range of values considered as normal by the manufacturer is chagned. Conclusions: On the basis of analyzed IgE total determinations it has been established that the manufacturere's standards for children aged from 2 to 3 years are overestimated, whereas the population standards seem to be underestiamted. Therefore the determination of IgE total normal value range requires further studies.

945/2523

Tytuł oryginału: Evaluation of lymphocyte subsets and NK cells in septic children.
Autorzy: Wiśniewska-Ligier Małgorzata, Woźniakowska-Gęsicka Teresa, Zeman Krzysztof, Banasik Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR119-CR124, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Disorders of lymphocyte subpopulations counts and NK cells may not only contribute to impaired elimination of pathogens, but also be an important factor in the development of generalized inflammatory reaction. The aim of the paper was the analysis of subpopulations of lymphocytes and NK cells in children with sepsis and after recovery. Material/Methods: The study comprised 25 septic children (17 infants and 8 children aged from 1 to 3 years). The control group consisted of 25 healthy children. The study was conducted in the initial phase of sepsis and then repeated 2 to 3 months after recovery. In all the children, the percentage and absolute numbers of basic lymphocyte subsets and NK cells in blood serum were evaluated. Results: In the course of sepsis, the absolute number of activated lymphocytes T in the youngest children was higher in comparison with the control gorup. After revovery, the percentage and absolute counts of CD4 lymphocytes in all the children and CD3/CD4 lymhocytes ratio in the youngest children were considerably lower than in the control gorup. The percentage and absolute number of NK cells in the youngest children after recovery were higher in comparison with the control group. Conclusions: In children who underwent sepsis, depression traits of cellular immunity are observed. The occurrence of defensive system disorders after recovery may be an important factor in recurrent infections and creates the necessity of immune system monitoring in children with the hisotry of sepsis for the purpose of prevention.

946/2523

Tytuł oryginału: Distribution of hepatitis C virus RNA in whole blood of patients with HCV infection and HBV-HCV coinfection.
Autorzy: Mazurek Urszula, Wilczok Tadeusz, Mazur Włodzimierz, Bednarek Ilona, Strzałka Barbara, Jurzak Magdalena, Laskowska-Lepiarczyk Ewa, Gonciarz Zbigniew
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CR125-CR130, tab., bibliogr. 23 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: Although HCV and HBV are essentially hepatotropic, several lines of evidence suggest that these viruses can infect other cells, also PBMC in most patients with chronic HCV. Material/Methods: The presence of HBV DNA and HCV-RNA was determined by a polymerase chain reaction (multiplex PCR and RT-PCR -nested PCR) in a group of patients with chronic liver disease. HCV-RNA was investigated in serum, plasma and peripheral blood mononculear cells (PBMC) while HBV-DNA only in serum or plasma. Results: Among 374 patients tested, HCB-RNA was detected in the venous blood of 208 patients; HCV RNA alone was detected in 154 patients and 54 patientwere co-infected by HCV and HBV. HBV-DNA was found in 128 of 374 patients, while infection by HBV only was found in 74 patients. It was also shown that in the presence of HBV the replication ability of HCV is lower (p = 0.085, Goodman-Kruskal Gamma = 0.561 and YuleQ = 0.5610). Conclusions: Since coexistence of HBV adn HCV is not a rare cases, diagnostics of hepatitis cannot be limited to detection of one type of the virus only. Nisinterpretation of the virus type that caused the infection may lead to serious complications, especially in those cases when interferone is used for treatment.

947/2523

Tytuł oryginału: An open-label study to evaluate the safety, tolerability and efficacy of rivastigmine in patients with mild to moderate probable Alzheimer's disease in the community setting.
Autorzy: Bilikiewicz Adam, Opala Grzegorz, Podemski Ryszard, Pużyński Stanisław, Łapin Joanna, Sołtys Krzysztof, Ochudło Stanisław, Barcikowska Maria, Pfeffer Anna, Bilińska Małgorzata, Paradowski Bogusław, Parnowski Tadeusz, Gabryelewicz Tomasz
Źródło: Med. Sci. Monitor 2002: 8 (2) s.PI9-PI15, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Long-term safety and efficacy of Exelon (rivastigmine) was evaluated in a multi-center open label study of 62 patients with probable mild to moderated Alzheimer's disease living in community setting. Material/Methods: The patients started treatment with 1.5 mg bid (3 mg/day) Exelon and were scheduled to receive doses of 1.5 mg bid Exelon escalating on a biweekly basis. The patients were maintained on the highest tolerated dose within the assigned dose range 1.5 - 60.0 mg bid (3 - 12 mg/day) for the rest of the study. Evaluations were scheduled at biweekly intervals for the first 8 weeks and subsequently at study weeks 12, 18 and 26. Effects of Exelon on cognition were evaluated using the mini-mental state examination (MMSE) and selected items of Alzhemier's disease assessment scale (ADAS-cog) and the staging ot the disease was measured using the global deterioration scale (GDS). Safety was monitored by physical examinations, vital signs, laboratory tests, ECG recording and by the assessment of adverse events. Resutls: 55 patients completed the study (89 p.c.). Patients treated for 26 weeks showed the mean MMSE, ADAS-cog and GDS scores close to baseline values (p = NS) with no improvement and no deterioration. Exelon was generally well tolerated with 11 p.c. of patients withdrawing due to adverse events. The most frequently reported adverse events related to the gastrointestinal tract. Conclusions: In conclusion, the study data indicate that treatment with Exelon is safe, generally well tolerated and inhibits the progression of cognitive decline in patients wtih mild to moderate Alzheimer's disease over 26 weeks of treatment.

948/2523

Tytuł oryginału: Clinical outcomes of silicon carbide coated stents in patients with coronary artery disease.
Autorzy: Kalnins Uldis, Erglis Andrejs, Dinne Iveta, Kumsars Indulis, Jegere Sanda
Źródło: Med. Sci. Monitor 2002: 8 (2) s.PI16-PI20, tab., bibliogr. 13 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: According to the literature, the stent thrombosis and restenosis rate after coronary angioplasty (PTCA) can be reduced by using stents covered with biologically active substances. The aim of our study was to investigate clinical outcomes of PTCA alone, conventional stainless steel stents, and special Tenax stents covered by amorphous silicon carbide (a-SiC: H). Material/Methods: The study involved a retrospective cohort of 300 patients with coronary artery disease who underwent interventional treatment from 1998 to 2000. One hundred patients received a Tenax stend (BIOTRONIK, Germany) covered by a-SIC: H. For every patient who received a Tenax stent, one patient wit non-Tenax stenting and one patient with PTCA without stenting were randomly assigned. Results: In the Tenax group, 1 p.c. of the patients had an early coronary event due to subacute stent thrombosis, as compared to 2 p.c. in the non-Tenax group and 3 p.c. in the PTCA group. At 6-month follow-up, the difference in the frequency of major adverse cardiac events was more express between the PTCA group and both groups of patients with stents (32 p.c. vs. 20 p.c.; p = 0.053; 32 p.c. vs. 14 p.c.; p = 0.002) in comparison with the 30-day and 3-month results. In the non-Tenax stenting group more events were detected than in the Tenax group (20 p.c. vs 14 p.c.; p = 0.259), but the diffrence was not statistically significant. Conclusion: The results strongly suggest the protective qualities of a-SiC: H stents in reducing early and late coronary events. It can be speculated that Tenax stents attenuate the progression of endothelial growth at the site of intervention.

949/2523

Tytuł oryginału: PACAP and VIP in the photoneuroendocrine system (PNES).
Autorzy: Kves Katalin, Vereczki Viktória, Kausz Maria, Kantor Orsolya, Molnar Judith, Nemeskri Agnes, Heinzlmann Andrea, Szabó Enik, Szabó Flóra, Fógel Kristóf, Lakatos Andras, Szeiffert Gabor, Arimura Akira
Źródło: Med. Sci. Monitor 2002: 8 (2) s.SR5-SR20, il., tab., bibliogr. 91 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The prewsence of PACAP and VIP was demonstrated in all the four levels of the photoneuroendocrine system (PNES) with the use of immunohistochemistry (IHC), radioimmunoassay (RIA), anterograde and reterograde tracing techniques, and cell immunoblot assay (CIBA). Both peptides paly a physiological role in the PNES. According to our results both PACAP and VIP are involved in the regulation of the gonadotrop hormone secretion and their inhibitory role may be mediated through the neuronal chain of the PNES. Abbreviations: CIBA - cell immunoblot assay, CRF - corticotrop hormone-releasing hormone, FG - fluorogold, FSH - follicle stimulating hormone, GRP - gastrin releasing peptide, HRP - horse-radish peroxidase, icv - intracerebroventricular, IHC - immunohistochemistry, iv - intraveous, LH - luteinizing hormone, LHRH - luteinizing hormone-releasing hormone, ME - median eminence, MSG - monosodium glutamate, PACAP - pituitary adenylate cyclase activating polypeptide, PhA-L - Phaseolus Vulgaris Leucoagglutinin, PHI - peptide histidine isoleucine, PNES - photoeuroendocrine system, PVN - paravetricular nucleus, RIA - radioimmunoassay, sc - subcutaneous, SCN suprachiasmatic nucleus, SON - supraoptic nucleus, VIP - vasoactive intestinal polypeptide.

950/2523

Tytuł oryginału: The course of vision disturbances in a patient with the MELAS syndrome.
Autorzy: Pachalska Maria, DiMauro Salvatore, Formińska-Kapuścik Maria, Kurzbauer Henryk, Talar Jan, MacQueen Bruce Duncan, Pawlicka Iwona, Jeleńska-Szyguła Irena
Źródło: Med. Sci. Monitor 2002: 8 (2) s.CS11-CS20, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: This article describes the neuropsychological and ophthalmic symptoms presented by a patient with MELAS, a mitochondrial cytopathy. This rare disease is characterized by a remitting-relapsing course against the background of a slowly progressive degenerative process. Case report: The patient is a 22-year-old Polish female, with initial onset of symptoms in 1994; the clinical diagnosis of MELAS was established in 1998, and confirmed in 2000 by the discovery of a novel mtDNA mutation. Her visual acuity fluctuates from near-normal to near-blindness, often changing dramatically within a matter of weeks; the visual field has more or less steadily narrowed to lunate. Visual evoked potentials show sporadic distrubances, while the nerve fiber layer shows significant attenuation. The evidence points to a complex etiology, involving both cortical damage and attenuation of the optic nerves and neural pathways. A similar two phase pattern - episodic disturbances with rapid spontaneous revovery against a background of progressive deterioration - occurs in neuropsychological testing, which reveals progressive dementia and episodic aphasia. Conclusions: The peculiiar pathomechanism of MELAS results in simultaneous insults to various parts of the central and peripheral nervous systems, creating the complex and highly variable pattern seen in this patient. In clinical practice care should be taken not to overlook the possible significance of such a pattern appearing in various systems and on varying levels.

951/2523

Tytuł oryginału: Tumor markers in metastatic disease from cancer of unknown primary origin.
Autorzy: Milović Marijana, Popov Ivan, Jelić Svetislav
Źródło: Med. Sci. Monitor 2002: 8 (2) s.MT25-MT30, il., tab., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: About 5 - 10 p.c. of cancer patients suffer from cancer of unknown primary (CUP). Sopisticated diagnostic techniques have not improved diagnostic efficacy for these patients. The aim of our analysis was to evaluate serum levels of four tumor markers and to relate them to the histology, number, and sites of metastases, the response to chamotherapy, and survival. Material/Methods: Blood samples from 46 patients with CUP were assayed for CEA, CA 19-9, CA 15-3 and CA 125. Specific IMx tumor marker assays (Abbott) were used to measure tumor marker quantities. Results: No significant differences were detected between the mean levels of all markers for the two histological types (adenocarcinoma vs. undifferentiated carcinoma). The comparison of tumor marker values in patients with one metastatic location to those with two or more showed no significant differencnes. We found no differences in marker values between patients with disease sites in the thorax vs. those with abdominal metastases. The comparison of marker negative, CEA positive and non-CEA positive patients failed to reveal significant differences in response rate. The comparison of CEA positive and marker negative patients showed significant differences in survival (log-rank, p = 0.02). Conclusion: Our results suggest that patients with CUP have a nonspecific overexpression of serum tumor markers and that routine use of these markers does not offer diagnostic assistance. Neverthelless, the pattern of expression of some biological markers, such as CEA, could have prognostic significance for survival.

952/2523

Tytuł oryginału: Dynamic MR imaging of soft tissue tumors with assessment of the rate and character of lesion enhancement.
Autorzy: Tacikowska Małgorzata
Źródło: Med. Sci. Monitor 2002: 8 (2) s.MT31-MT35, tab., bibliogr. 22 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Background: The aim of this study was to analyze the diagnostic usefulness of dynamic MRI with determination of the coefficient of enhancement rate and the character of tumor enhancement, and to assess both parameters in the differentiation of malignant lesions. Material/Methods: The material consisted of 45 patients (30 sarcomas, 15 non-malignant lesions), age 16 - 64 years. MRI was done usin an Elscint 2T unit, gradient echo techniques, apex angle 80ř. The repetition time (TR) was 80 - 200 ms, the echo time (TE) was 2 - 6 ms, I excitation; the acquisition time (TA) was 70 - 80 ms. The coefficient of tissue enhancement rate was calculated in the region of interest, and expressed as percent per second (erc p.c./s.) The limit value of erc p.c./s was determined. The sensitivity and specificity of MRI were calculated in the differentiation of malignant tumors. The method of contrast filling of the tumors was assessed in successive phases after administration of gadolinium Gd-DTPA. Results: Dynamic MRI with determination of the index of tumor enhancement rate is highly sensitive (93 p.c.) and specific (73 p.c.) in the differentiation of malignant and benign lesions. The usefulness of the assessment of tumor enhacnement character was not confirmed, since the sensitivity and specificity wre 73 p.c. and 33 p.c. Dynamic MRI with determination of erc p.c./s and tumor enhancement character is highly sensitive (93 p.c.) and specific (87 p.c.). Conclusions: Dynamic MRI with determination of erc p.c.; s and tumor enhancement character is the best method for differential diagnosis.

953/2523

Tytuł oryginału: MR proton spectroscopy in liver examinations of healthy individuals in vivo.
Autorzy: Tarasów Eugeniusz, Siergiejczyk Leszek, Panasiuk Anatol, Kubas Bożena, Dzienis Wojciech, Prokopowicz Danuta, Walecki Jerzy
Źródło: Med. Sci. Monitor 2002: 8 (2) s.MT36-MT40, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Magnetic resonance proton spectroscopy (1H MRS) is a non-invasive method that provides in vivo measurement of metabolic concentrations in body tissue. However, relatively little is known about the potential of 1H MR spectoscopy fot the qualification of liver metabolites. The aim of this study was to determine the usefulness of 1H MRS in establishing the metabolic pattern of normal human liver. Material/Methods: Proton specrtoscopy of the liver, using the Picker system, Edge Eclipse 1.5T, with whole-body coil and PRESS 35 sequence, was conducted in a group of 24 healthy volunteers. In all subjects we also evaluated the thckness of the subcutaneous fat tissue in MR images and body mass index. Results: The presence of lipid resonances, phosphoesters (Pe), glycogen/glucose (Glc), and glutamine/glutamate (Gln) were observed in the spectra. Total lipid concentration, CH3 and (CH2)n of the lipid resonancs were higher in the men than in the woemn, while the metabolite contents in the CH2=CH-CH2 gorups of lipids, Pe, Glc and Bln peaks were similar in both genders. We observed statistically significant positive correlation, more apparent in the gorup of men, between TL concentration and BMI. There was no statistically significant correlation between lipid total concentration and age. Conclusion: Proton spectorscopy enables the quantitative evaluation of lipid contents in the liver. The correlation between liber triglyceride contents and body mass ndex shows that the tendency to obesity is also connected with lipid accumulation in the liver.

954/2523

Tytuł oryginału: Tonal nitric oxide and health: anti-bacterial and -viral actions and implications for HIV.
Autorzy: Benz Danielle, Cadet Patrick, Mantione Kirk, Zhu Wei, Stefano George B.
Źródło: Med. Sci. Monitor 2002: 8 (2) s.RA27-RA31, tab., bibliogr. 46 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Nitric oxide has been shown to have important physiological regulatory roles, i.e., vasodilation, neurotransmitter release, etc. Now, we review its role as an antibacterial and antiviral agent. Nitric oxide has also been identified as an improtant factor in the development of nonspecific immunity. And accordingly, nitric oxide synthase (NOS), the catalytic wnzyme producing nitric oxide, is a key element in the protective activities of nitric oxide. The expression of inducible (i) NOS is regulated by cytokines. iNOS-derived nitric oxide was found to contribute to both early and late phases of antibacterial activity. Enzymes, such as proteases (reverse transcriptases, and ribonucleotide reductase, etc.) containing cysteine residues, appear to be targets for nitric oxide nitrosylation, as well as viral-encoded transcription factors that are involved in viral replication. It would appear than thiss multifunctional signaling molecule is not only involved with signaling between cells, it also appears to maintain the immediate environment free of microbial agents.

955/2523

Tytuł oryginału: Hepatocyte proliferation in health and in liver failure.
Autorzy: Rozga Jacek
Źródło: Med. Sci. Monitor 2002: 8 (2) s.RA32-RA38, il., bibliogr. 58 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Mammalian liver possesses an extraordinary capacity for compensatory growth in response to conditions that induce cell loss by physical, infectious, or toxic injury. In normal animals and humans, it is a tightly regulated process of both hypertrophy and hyperplasia involving different liver cell populations and a finely tuned interplay between growth factors, cytokines, extracellular matrix components and other regulators. The regeneration response is maximal when two-thirds of teh liver is resected. When a lesser amount of parenchyma is removed, residual liver grows more slowly. Resections exceeding two-thirds of the liver mass also retard andn diminish both DNA synthesis and mitotic activity and subtotal (90 p.c.) hepatectomy invariably results in the death of rats without regeneration. The underling mechanicms of liver growth inhibition are poorly understood. In particular, only a few studies exist that provide insight into the mechanisms that control regeneration after extensive heaptocyte loss. In this regard, the role of growth-regulatory factors and other compounds that accumulate in the blood circulation as a result of hepatic insufficiency and liver cell death remains unclear. We have initiated studies of these mechanisms and demonstrated that in rats with low (10 p.c.) hepatocyte mass, a marked and sustained elevation of blood IL-6, HGF adn TGF-á1 levels was associated with lack of heaptocyte proliferation and suppression of Stat3 DNA binding. While searching for the possible cause of inhibited IL-6/Stat 3 signaling, we found that IL-6 receptor (IL-6R & gp130) was preserved, that nuclear Stat3 protein content was lowered, and that IL-6/Stat3 pathway inhibtors (SOCS-1, PIAS3) were induced during the pre-replicative Go-G1 period.

956/2523

Tytuł oryginału: Regulation of renal tubular sodium transport by cardiac natriuretic peptides: two decades of research.
Autorzy: Bełtowski Jerzy, Wójcicka Grażyna
Źródło: Med. Sci. Monitor 2002: 8 (2) s.RA39-RA52, il., bibliogr. 188 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: This review presents the current state of our knowledge regarding the regulation of renal tubular sodium transprot by natriuretic peptides, with special emphasis on recent findings in this field. Natriuretic peptides constitute a complex system involved in the regulation of sodium balance and blood pressure. The natriuretic peptide family consists of atrial peptides, such as atrial natriuretic factor (ANF, ANP90-126), long-acting natriuretic peptide (ANP1-30), vessel dilator (ANP31-67) andn kaliuretic peptide (ANP79-98), as well as brain or B-type natriuretic peptide (BNP), C-type natriuretic peptide (CNP) and urodilatin. Natriuretic peptides act on target cells through A-type and B-type receptors and stimulate cyclic GMP synthesis. ANF stimulates natriuresis mainly by inhibiting soidum reabsorption in the inner medullary collecting duct. The effect results from coordinate inhibition of apical sodium channels and basolateral Na+, K+-ATPase. Additional effects on sodium transport occure in more proximal nephron segments and on glomerular filtration when hormone concentration is elevated. BNP and urodilatin share the same mechanism of action. CNP synthetized in several nephron segments acts through specific B-type natriuretic peptide receptors, which are also expressed in renal tubule, but have a different distribution than A-type receptors. ANP1-30, ANP51-67 and ANP79-98 decrease Na+, K+-ATPase activity in tubular cells through a prostaglandin E2-deendent mechanism.

957/2523

Tytuł oryginału: Bakers' asthma - how far are we from prevention?
Tytuł polski: Astma piekarzy - jak daleko do prewencji?
Autorzy: Walusiak J[olanta], Pałczyński C.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.5-7, bibliogr. 22 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Astma piekarzy wciąż należy do najczęstszych chorób zawodowych. Autorzy omawiają aktualne problemy dotyczące alergii zawodowej dróg odechowych u piekarzy, ze szczególnym uwzględnieniem czynników ryzyka i zależności pomiędzy występowaniem objawów alergicznych a poziomime narażenia.

958/2523

Tytuł oryginału: A case of Churg-Strauss syndrome and montelukast therapy.
Tytuł polski: Przypadek zespołu Churga-Strauss i leczenie montelukastem.
Autorzy: Świerczyńska M., Mastalerz L., Niżankowska-Mogilnicka E.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.9, 11-12, bibliogr. 10 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: W pracy przedstawiono przypadek 28-letniego chorego z zespołem Churga-Strauss (Churg-Strauss syndrome, CSS), który pojawił się w trakcie leczenia montelukastem. Pacjent został przyjęty z powodu duszności astmatycznej, gorączki i kaszlu. Przy przyjęciu stwierdzono leukocytozę oraz eozynofilię. W badaniu radiologicznym klatki piersiowej stwierdzono nacieki w górnych i środkowych polach płucnych. Biopsja oskrzela była prawidłowa. W popłuczynach pęcherzykowo-oskrzelowych wykazano Citobacter freundii. Ze względu na hipereozynofilię rozpatrywano wiele jej przyczyn, w tym również CSS, objawy nie spełniały jednak wszystkich kryterów diagnostycznych CSS. Ostatecznie rozpzonano astmę alergiczną oraz bakteryjne zapalenie płuc. Do leczenia włączono antybiotyki oraz steroidy doustne. Stan chorego uległa poprawie i nacieki płucne cofnęły się. Po wypisie dawki steroidów doustnych stopniowo zmniejszano, a następnie zamieniono na steroidy wziewne. Po 6 miesiącach od wypisu rozpoczęto leczenie montelukastem. W trakcie terapii tym lekiem stan chorego uległ pogorszeniu i pojawiły się objawy pozapłucne. Zespół Churga-Strauss potwierdzono przez biopsję zmian skórnych. Do leczenia włączono doustne steroidy z dobrym skutkiem. W dyskusji omówiono możliwe przyczyny coraz częstszego pojawiania się przypadków CSS w trakcie terapii lekami przewciwleukotrienowymi.

959/2523

Tytuł oryginału: Cells and mediators in nasal and bronchoalveolar lavage fluid from patients with occupational asthma due to diisocyanates.]
Tytuł polski: Komórki i mediatory w popłuczynach nosowych i oskrzelowo-pęcherzykowych u chorych na astmę oskrzelową wywołaną przez izocyjaniany.
Autorzy: Krakowiak A[nna], Pałczyński C., Walusiak J., Ruta U., Wittczak T., Gruchała J.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.13-19, il., tab., bibliogr. 24 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: W badaniu brało udział 30 osób chorych na astmę oskrzelową zawodowo narażonych na izocyjaniany. U każdego pacjenta przeprowadzono wywiad chorobowy, badanie przedmiotowe, wykonano punktowe testy skórne, oznaczono całkowite stężenie IgE w surowicy i występowanie antygenowo swoistych przeciwciał IgE oraz badanie spirometryczne. Dodatkowo analizowano zmiany w składzie morfologicznym i biochemicznym oraz stężenia mediatorów w popłuczynach nosowych i oskrzelowo-pęcherzykowych przed i 25 h po prowokacji izocyjanianami i placebo. Średnia wartość całkowitego stężęnai IgE wynosiła 4r kU/I, u 10 osób stwierdzono IgE antygenowo swoiste. Występowanie podwójnej reakcji alergicznej odnotowano u 10 osób, zaś u pozostałych potwierdzono izolowaną reakcję późną po prowokacji izocyajnianami. Jedna osoba skarżyła się na objawy nieżytu nosa po prowokacji. Znamienny statystycznie wzrost odsetka eozynofilów i neutrofilów oraz albumin odnotowanoe w popłuczynach nosowych i oskrzelowo-pęcherzykowych w 25 h po prowokacji izocyjanianami. Nie obserowowano istotnych zmian w zakresie analizowanych parametrów po prowokacji placebo. Prowokacji wziewna izocyjanianami wywołała podobne zmainy zarówno w składzie komórkowym, biochemicznym, jak i w zmianach stężeń medatorów w górnych i dolnych drogach; reakcją alergiczną najczęściej odnotowywana po prowokacji izocyjanianami była izolowana późna reakcja alergiczna.

960/2523

Tytuł oryginału: The evaluation of neutrophils functions in patients with atopic dermatitis.
Tytuł polski: Przydatność oceny czynności granulocytów obojętnochłonnych u chorych na atopowe zapalenie skóry.
Autorzy: Samochocki Z[bigniew], Zabielski S., Paluchowska E.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.20-24, tab., bibliogr. 28 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Nawrotowe zakażenia bakteryjne skóry są cechą atopowego zapalenia skóry (AZS). Jedną z przyczyn tego zjawiska mogą być zaburzenia czynności PMN. Celem pracy była ocena zdolności granulocytów obojętnochłonnych (polymorphonuclear, PMN) krwi obwodowej i puli skórnej do migracji, redukcji i pochłaniania oraz ocena przydatności tych testów w przewidywaniu przebiegu AZS. Badaniami objęto 32 chorych na AZS (f18 K, 14 M, w wieku średnio 23,6 ń 6,2 lat) i 32 osoby zdrowe (13 K, 19 M, w wieku średnio 23,4 ń 2,7 lat). Ocenę czynności granulocytów obojętnochłonnych skóry i krwi obwodowej dokonano metodą Clausena na podstawie ich zdolności do spontaniczenj migracji oraz redukcji NBT i pochłaniania lateksu - testem Parka. Uzyskane wyniki poddano analizie statystycznej. Stwierdzono, że u chorych a AZS obserwuje się częściowe zaburzenie funkcji PMN, czego wyrazem jest ich zmniejszone zdolność do spontanicznej migracji. Określenie tego parametru może być pomocne w rozpoznawaniu AZS, natomiast nie jest przydatne w prognozowaniu przebiegu choroby. Podwyższona wartość testu NBT granulocytów tylko puli skórnej może przemawiać za zachowaną ich zdolnością bakteriobójczą i świadczyć o ich pobudzeniu zależnym od kolonizacji bakterii na powierzchni skóry.

961/2523

Tytuł oryginału: Interrelation of cytokines and their association with acute phase proteins in Hodgkin's disease and in rheumatoid arthritis.
Tytuł polski: Współzależność cytokin i ich związek z białkami ostrej fazy w chorobie Hodgkina i reumatoidalnym zapaleniu stawów.
Autorzy: Karter Y[esari], Uzun H., Tunckale A., Aydin S., Ertrk N. T., Aran S., Yaldiran A., ztrk E.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.25-28, tab., bibliogr. 16 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

reumatologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Kilka cytokin uznano za silne immunologiczne mediatory w chorobie Hodgkina (HD) i w reumatoidalnym zapaleniu stawów (RA). Wykorzystując metodę ELISA określono stężenia cytokin -IL-1á, IL-6, TNFŕ i białek o strej fazy, a w tym CRP, á2-mikrogloulinę, ceruloplazminę, ferrytynę, fibrynogen i haptoglobinę w grupie chorych z HD nieleczonych (6 kobiet, 7 mężczyzn, śr. wiek 34 +/- 16) i z RA (7 kobiet i 5 mężczyzn, śr. wiek 43 +/- 12). Charakteryzowali się oni zwiększonym wskaźnikiem opadania erytrocytów (102,60 +/- 23 mm/h i 103,72 +/- 15,09 mm/h - odpowiednio.Poszukowano koreloacji i związku badanych cytokin z białkami ostrej fazy. W analizie statystycznej zastosowano metodę ANOVA i metodę korelacji. Stężenia IL-1 zaró no u chorych na HD (f26,17 +/- 33,00 pg/ml), jak i z RA (26,04 +/- 22,67 pg/ml) były znamiennie większe niż w grupie kontrolnej (3,58 +/- 0,15 pg/ml) (p 0.001). Średnie stężeneia IL-6 i TNFŕ u chorych na HD wynosiły 89,6 +/- 104,36 pg/ml i 77,52 +/- 32,14 pg/ml - odpowiednio, przy wartościach w grupie kontrolnej 3,53 +/- 0,93 pg/ml i 6,35 +/- 2,29 pg/ml (p 0,001). Podobnie u chorych na RA wartości IL-6 wynosiły 66,52 +/- 109,32 pg/ml TNFŕ 71,30 +/- 18,60 pg/ml. U chorych na HD stężenia á2-mikroglouliny (5,83 +/- 5,26 mg/l), CRP (9,25 +/-5.69 mg/l) i haptoglobiny (370,12 +/- 326,19 mg/dl) były znamiennie większe. Podobne zmainy stwierdzono u chorych na RA, u których stężenie á2-mikroglobuliny (6,58 +/- 7,89 mg/l), CRP (9,98 +/- 5.36 mg/l), ceruloplazminy (66,50 +/- 88,85 mg/dl) i fibrynogenu (483,09 +/- 173,72 mg/dl) były rówież znamiennie większe. Wykazano liniową korelację...m

962/2523

Tytuł oryginału: Studies on IL-6, IL-8, bFGF, sTNFRI, sTNFRII and sIL-2R in serum of patients with colorectal cancer.
Tytuł polski: Badania IL-6, IL-8, bFGF, sTNRFI, sTNFRII i sIL-2R w surowicy chorych na raka jelita grubego.
Autorzy: Grotowski M[aciej], Piechota W. N., Maruszyński M.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.29-35, il., tab., bibliogr 28 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Cytokiny są grupą mediatorów o zazwyczaj krótkim szeregu białka, które odgrywają ważną rolę w rozwoju, działaniu i kontroli komórek odporności i wielu innych układów. Ekspresja cytokin w surowicy u chorych na nowotwory złośliwe przewodu pokarmowego wciąż nie jest dobrze poznana. Celem pracy było zbadanie w surowicy u chorych na gruczolakoraka jelita grubego przedoperacyjnych stężeń IL-6, IL-8, podstawowego czynniak wzrostu fibroblasta (vFGF) i rozpuszczalnych (s) receptorów (R) czynnika martiwcy guza - TNF (sTNFRI i sTNFRII) oraz IL-2 (sIL-2R). Oceniano również czułość badanych czynników i związek między ich stężeniami a stopniem zaawansowania nowotworu. Badaniem objęto 81 chorych z rozpoznanym histologicznie przed operacją gruczolakorakiem jelita grubego (grupa chorych na raka) oraz 40 zdrowcyh ochotników (grupa kontrolna). Badane czynniki mierzono, stosując zestaw ELISA. Używając metody percentylowej, w oparciu o wyniki grupy kontrolnej obliczano czułość badanych czynników. Stwierdzono, że w grupie chorych w surowicy występowało zanczące zwiększenie ich stężenia, które wynosiło: IL-6 - 5,9, IL-8 - 4,4, bFGF - 3,4, sTNFRI - 1,5, sTNFRII - 1,4 i sIL-2R - 2,0 pg/ml i były 2-krotnie większe w porównaniu z grupą kontrolną (p 0,001). Wartości graniczne w grupie kontrolnej wynosiło: IL-6 - 4,5; IL-8 - 21,1; bFGF - 6,25; sTNFRI - 2254; sTNFRII - 3010 i sIL - 2R - 1676 pg/ml. Czułość IL-6 wynosiła 37 proc., IL-8 - 65,4 proc., bFGF - 25,9 proc., sTNFRI - 30,9 proc., sTNFRII - 50,6 proc. i SIL-2R 71, 6 proc. Stężenia IL-6, IL-8 i sIL-25 dobrze korelowały z progresją nowotworu (p 0,05). Oznaczenie poziomów...

963/2523

Tytuł oryginału: Comparison of CEA, IL-6, IL-8, bFGF, sTNFRI, sTNFRII and sIL-2R levels in serum of colorectal cancer patients.
Tytuł polski: Porównanie stężeń antygenu CEA, IL-6, IL-8, bFGF, sTNFRI, sTNFRII i sIL-2R w surowicy u chorych na raka jelita grubego.
Autorzy: Grotowski M[aciej], Piechota W. N.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.36-43, tab., bibliogr. 26 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Antygen CA jest najpowszechniej używanym antygenem u chorych na raka jelita grubego do rozpoznawania i monitorowania przebiegu choroby. Jego ograniczona czułość i swoistość powodują, że jest zbyt słaby do wykrywania wczesnych postaci tego nowotworu. Ostanio liczne badania zwróciły uwagę na czynniki regulujące odporność, które mogą ułatwić wzrost nowotworów. W tym badaniu analizowaiśmy przedoperacyjne stężenia antygenu CEA, interleukin (IL-6 i IL-8), podstawowego czynnika wzrostu fibroblasta (bFGF) oraz rozpuszczalnych receptorów czynnika martwicy guza (sTNFRI i sTNFRII) i IL-2. Określaliśmy ich czułość oraz współzależność między zasięgiem ich wzrostu a czynnikami kliniczno-patologicznymi. Porównywaliśmy też częstość występowania dodatnich wyników antygenu CEA, badanych cytokin i rozpuszczalnych receptorów. Badaniem objęto 81 chorych na raka jelita grubego (grupa z rakiem) oraz 40 zdrowych osób (grupa kontrolna). Stopień zaawansowaniaai nowotworu oceniano wg klasyfikacji Dukes'a. Stężenia antygenu CEA mierzono w grupie z rakiem używając kitów MEIA. Za górną granicę normy przyjęto wartość 3 ng/ml. W obu gruchach stężenie cytokin i rozpuszczalnych receptorów mierzono używając kitu ELISA. W oparciu o wyniki grupy kontrolnej ustalono normy dla badanych cytokin i rozpuszczalnych receptorów oraz określono ich czułość. Czynnikami kliniczno-patologicznymi były: płeć, wiek, typ histologiczny, przerzuty do wątroby, rozsianie otrzewnowe, zajęcie węzłów chłonnych, umiejscowienie nowotworu, trwanie objawów i stopnie Dukes'a. Ekspresja antygenu CEA, IL-8 oraz sIL-2R dobrze korelowała z analogicznymi...

964/2523

Tytuł oryginału: Investigations on the role of immunological factors in aseptic loosening of total cemented and uncemented hip prosthesis. [P.] 3: Morphological investigations.
Tytuł polski: Badania nad rolą czynników immunologicznych w obluzowaniu aseptycznym całkowitej cementowej i bezcementowej protezy stawu biodrowego.
Autorzy: Denys P[aweł], Szmigiel A., Żołyński A.
Źródło: Int. Rev. Allergol. Clin. Immunol. 2002: 8 (1) s.44-49, il., bibliogr. 21 poz., sum.
Sygnatura GBL: 313,209

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Dokumentacja morfologiczna obejmuje wyniki badań histopatologicznych uzyskanych w mikroskopie świetlnym, wyniki badań immunohistochemicznych i mikroskopowo-elektronowych. Potwierdzono opisywane w piśmiennictwie aktywacje i proliferacje makrofagów wokół mas martwiczych i fragmentów polietylenu. Znaleziono także dane potwierdzające udział komórkowych reakcji immunologicznych w procesie obluzowania protezy: obecność komórek limfocytarnych i plazmocytów w tkankach strefy styku protezy.

965/2523

Tytuł oryginału: Significant variations of responsiveness of rat gut smooth muscles to glutamate.
Autorzy: Milovanovic Dragan R., Matovic Milovan, Milicic Biljana, Jankovic Slobodan M.
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.507-511, il., tab., bibliogr. 8 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Our four-year study, conducted on isolated rat stomach and rectum strips from adult rats of the same brood in five series, demonstrated for the first time notable variations of the responsiveness of the gut smooth muscles to glutamate, the findings that point to strong variability of glutamate contractile action which could lead to inconsistencies and should be taken into acount in the future research.

966/2523

Tytuł oryginału: Azathioprine-induced fatal macrocytic anemia in rabbits.
Autorzy: Al-Safi Saafan, Tashtoush Bassam, Abdul-Razzak Khalid
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.513-516, tab., bibliogr. 40 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: Azathioprine (AZA) is used clinically sometimes at high doses for short-term therapy to treat acute rejection of kidney allograft or to desensitize hypersensitive patients to it. The delayed consequences of this approach had not been well investigated. Therefore, in this study we have investigated the delayed consequences of high-dose short-term AZA administration in rabbits. Our results showed that oral administraton of AZA (10 mg/kg/day) to rabbits for two weeks induced reversible thrombocytosis and delayed fatal macrocytic anemia. Moreover, neither the hemoglobin level nor the the white blood cell count was affected by AZA. The solvent of AZA had no effect onblood cell counts and hemoglobin levels. We can conclude that although high-dose AZA therapy may not induce immediate and significant changes in blood picture, delayed fatal macrocytosis may occur.

967/2523

Tytuł oryginału: SIB 1893 possesses pro- and anticounvulsant activity in the electroshock seizure threshold test in mice.
Autorzy: Łuszczki Jarogniew, Borowicz Kinga K., Czuczwar Stanisław J.
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.517-520, il., bibliogr. 9 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: SIB 1893, a non-competitive antagonist of group I metabotropic glutamate receptor sutype 5, administered at the doses ranging between 0.5 - 2 mg/kg markedly lowered the electorconvulsive thershold, whereas if applied at the higher dose of 40 mg/kg, it significantly raised the threshold for electroconvulsions in mice, exhibiting both pro- and anticonvulsive properties in this test.

968/2523

Tytuł oryginału: Electroconvulsions elevate the levels of lipid peroxidation products in mice.
Autorzy: Rola Radosław, Świąder Mariusz, Czuczwar Stanisław J.
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.521-524, il., bibliogr. 14 poz., sum.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The objective of this study was to determine whether electroconvulsions lead to excessive lipid peroxidation. The concentrations of the conjugated dienes (CD) and malonyl dialdehyde (MDA) in the homogenates of the brains after seizures induced with 25 mA current (MES) measured immediately after seizures were significantly higher in comparison with the control brains. There were no significant differences between control group and animals treated with multiple MES. Significant rise in CD concentrations was also observed at 1 h following MES. The results indicate that electroconvulsions may lead to the increased formation of lipid peroxidation products.

969/2523

Tytuł oryginału: VIII Conference on: Ethanol-drug interactions and pharmacological treatment of alcohol dependece, Łódź, October 18, 2002 - [abstracts].
Źródło: Pol. J. Pharmacol. 2002: 54 (5) s.525-548 - 8 Konferencja pt. Interakcje etanol-lek i farmakologiczne leczenie zależności alkoholowej Łódź 18.10. 2002
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

970/2523

Tytuł oryginału: Decreased level of kynurenic acid in cerebrospinal fluid of relapsing-onset multiple sclerosis patients.
Autorzy: Rejdak Konrad, Bartosik-Psujek Halina, Dobosz Beata, Kocki Tomasz, Grieb Paweł, Giovannoni Gavin, Turski Waldemar A., Stelmasiak Zbigniew
Źródło: Neurosci. Lett. 2002: 331 (1) s.63-65, il., bibliogr. 16 poz.
Sygnatura GBL: 305,936

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The present study was undertaken to measure cerebrospinal fluid (CSF) levels of kynurenic acid (KYNA) in patients with relapsing-onset multiple sclerosis (MS) during remission or not progressing for at least 2 months. In these patients the levels of CSF KYNA were found to be significantly lower compared with subjects with non-inflammatory neurological diseases, as well as those with inflammatory disease (median interquartile range): 0,41 (0.3 - 0.5) pmol/ml, n = 26 vs. 0.67 (0.5 - 1.1), n = 23, P 0.01 and 1.7 (1.5 - 2.6), n = 16, P 0.001, respectively). These results provide further evidence of the alterations in the kynurenine pathway during remitting-onset MS.

971/2523

Tytuł oryginału: Successful treatment of leukaemia cutis with cladribine in a patient with B-cell chronic lymphocytic leukaemia.
Autorzy: Robak E[wa], Robak T[adeusz], Biernat W[ojciech], Bartkowiak J[acek], Krykowski E[uzebiusz]
Źródło: Br. J. Dermatol. 2002: 147 (4) s.775-780, il., bibliogr. 27 poz.
Sygnatura GBL: 300,728

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Cutaneous presentation of B-cell chronic lymphocytic laukaemia (B-CLL) is uncommon, and the influence of skin changes on B-CLL prognosis is unclear. We report a patient with B-CLL Rai II, with multiple nodular skin infiltrations on the trunk, arms and thighs as well as constitutional symptoms, who was successfully treated with cladribine. The peripheral blood (PB) lymphocytes were CD19, CD20, Cd23 and CD5 positive, which confirmed the diagnosis of B-CLL. Skin biopsy of one of the lesions showed an intense infiltrate composed of small lymphocytes with no epidermotropism. These cells also showed the expression of CD19, CD20, CD23 and CD5 antigens similar to those presented on PB lymphocytes. Polymerase chain reaction performed on bone marrow lymphocytes and a lesional skin biopsy using consensus primers for immunoglobulin heavy-chain genes also showed the same monoclonal population of B lymphocytes both in the bone marrow and in the skin. The patient received four courses of cladribine 0ú12 mg/kg**-1 daily as a 2-h infusion for five consecutive days. The courses were repeated at monthly intervals. The lymphocytosis gradually decreased and the PB count normalized after three courses. At the same time, a significant decrease in the cutaneous symptoms was observed. The patient became free of skin tumours after the fourth course of cladribine; only slight discoloration at the previous sites of cutaneous infiltration remained. There was no relapse of laukaemia cutis during a further 7 months of observation.

972/2523

Tytuł oryginału: Protective properties of artichoke (Cynara scolymus) against oxidative stress induced in cultured endothelial cells and monocytes.
Autorzy: Zapolska-Downar Danuta, Zapolski-Downar Andrzej, Naruszewicz Marek, Siennicka Aldona, Krasnodębska Barbara, Kołodziej Blanka
Źródło: Life Sci. 2002: 71 (24) s.2897-2908, il., tab., bibliogr. 37 poz.
Sygnatura GBL: 304,948

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: It is currently believed that oxidative stress and inflammation play a significant role in atherogenesis. Artichoke extract exhibits hypolipemic properties and contains numerous active substances with antioxidant properties in vitro. We have studied the influence of aqueous and ethanolic extracts from artichoke on intracellular oxidative stress stimulated by inflammatory mediators (TNFŕ and LPS) and ox-LDL in endothelial cells and monocytes. Oxidative stress which reflects the intracellular production of reactive oxygen species (ROS) was followed by measuring the oxidation of 2', 7'-dichlorofluorescin (DCFH) to 2', 7'-dichlorofluorescin (DCF). Agueous and ethanol extracts from artichoke were found to inhibit basal and stimulated ROS production in endothelial cells and monocytes in dose dependent manner. In endothelial cells, the ethanolic extraxt (50 ćg/ml) reduced ox-LDL-induced intracellular ROS production by 60 p.c. (p 0,001) while aqueous extract (50 ćg/ml) by 43 p.c. (p 0,01). The ethanolic extract (50 ćg/ml) reduced ox-LDL-induced intracellular ROS production in monocytes by 76 p.c. (p 0,01). Effective concentrations (25-100 ćg/ml) were well below the cytotoxic levels of the extracts which started at 1 mg/ml as assessed by LDH leakage and trypan blue exclusion. Penetration of some active substances into the cells was necessary for inhibition to take place as juged from the effect of preincubation time. These results demonstrate that artichoke extracts have marked protective properties against oxidative stress induced by inflammatory mediators ans ox-LDL in cultured endothelial cells and monocytes.

973/2523

Tytuł oryginału: Influence of contralateral stimulation by two-tone complexes, narrow-band and broad-band noise signals on the 2f1-f2 distortion product otoacoustic emission levels in humans.
Autorzy: Lisowska Grażyna, Smurzyński Jacek, Morawski Krzysztof, Namysłowski Grzegorz, Probst Rudolf
Źródło: Acta Otolaryngol. 2002: 122 (6) s.613-619, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 300,481

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In order to test the frequency specificity of the efferent suppressive effect on otoacoustic emissions, changes in the 2f1-f2 distortion product otoacoustic emission (DPOAE) levels induced by contralateral stimuli of different spectra were measured in 10 normally hearing adults. Three types of contralateral stimuli were used: (I) a set of 6 pairs of pure tones with the same frequencies as used for DPOAE stimulation; (II) 6 narrow-band noise signals with cut-off frequencies equal to the frequencies of the primary tones used for DPOAE stimulation; and (III) broad-band noise with a bandwidth of 840-6,000 Hz. A small suppressive effect was observed mainly in the mid-frequency region. Broad-band noise was more effective at suppressing DPOAEs than narrow-band noises and two-tone complexes. Occasionally, small enhancements in DPOAE amplitudes were observed. Based on the results of this study, it is concluded that DPOAE changes induced by contralateral stimuli are not frequency-specific, and are too small to have routine clinical value.

974/2523

Tytuł oryginału: Short-time deoxynivalenol treatment induces metabolic disturbances in the rat.
Autorzy: Szkudelska Katarzyna, Szkudelski Tomasz, Nogowski Leszek
Źródło: Toxicol. Lett. 2002: 136 (1) s.25-31, il., tab., bibliogr. [36] poz.
Sygnatura GBL: 306,158

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Deoxynivalenol (DON) is produced by several Fusarium species and may contaminate feeds causing reduced food consumption and utilisation, reduced body weight gain and other unfavourable changes in animals. To verify if its adverse influence involves also hormonal and metabolic changes, the effect of DON on blood insulin, glucagon, leptin and metabolic parameters in growing Wistar rats was studied. Animals were treated subcutaneously with DON in the amount of 1 mg/kg b.w. After 3 days a significant increase in blood insulin, glucose and free fatty acids were observed in these animals in comparison to the control group. DON treatment caused an increment in glycogen depots and a reduction in triglycerides content in the muscle. Studies on isolated adipocytes revealed that DON (20 ćmol/l) slightly stimulated basal lipogenesis, whereas insulin-induced lipid synthesis and lipolysis were unchanged. Results obtained after subcutaneous DON administration indicate that its adverse effects in animals may partially result from metabolic disturbances evoked by this mycotoxin.

975/2523

Tytuł oryginału: Antimicrobial effect of Rhodiola rosea L. roots and callus extracts on some strains of Staphylococcus aureus.
Tytuł polski: Wpływ wyciągów z korzeni i kalusa Rhodiola rosea L. na niektóre szczepy Staphylococcus aureus.
Autorzy: Furmanowa M[irosława], Starościak B., Lutomski J., Kozłowski J., Urbańska N[atalia], Krajewska-Patan A., Pietrosiuk A[gnieszka], Szypuła W[ojciech]
Źródło: Herba Pol. 2002: 48 (1) s.23-31, il., tab., bibliogr. 15 poz., streszcz.
Sygnatura GBL: 303,599

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie polskie: Wyciągi z korzeni Rhodiola rosea L. zebranych ze środowiska naturalnego (Babia Góra, Karpaty) oraz Ogrodu Roślin Leczniczych Instytutu Roślin i Przetworów Zielarkich w Poznaniu, jak również wyciągi z kalusa in vitro, zmniejszały wzrost trzech szczepów Staphylococcus aureus: ATCC 6538 P, ATCC 25923 i NCTC 4163. Najbardziej wrażliwy był S. aureus NCTC 4163. Wyciągi z korzeni były bardziej aktywne. Powodowały większą strefę zahamowania wzrostu.

Streszczenie angielskie: Extracts of Rhodiola rosea obtained from intact plant roots harvested from the Medicinal Plants Garden of the Institute of Medicinal Plants in Poznań, from plants growing in the wild (Babia Góra in the Carpathian Mountains) and from callus cultivated in vitro reduced the growth of the three Staphylococcus aureus strains: ATCC 6538 P, ATCC 25923 and NCTC 4163. S. Aureus NCTC 4163 was the most sensitive to their action. The extracts of intact plant roots produced a higher growth inhibition zone than callus extracts.

976/2523

Tytuł oryginału: Application of supercritical CO2 extraction for separation of natural antibiotics from lichens.
Tytuł polski: Zastosowanie ekstrakcji CO2 w stanie nadkrytycznym do rozdziału naturalnych antybiotyków z porostów.
Autorzy: Lisickov K., Najdenova V., Zoltan D.
Źródło: Herba Pol. 2002: 48 (1) s.32-39, il., tab., bibliogr. 17 poz., streszcz.
Sygnatura GBL: 303,599

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie polskie: W badaniach użyto niekonwencjonalnej metody ekstrakcji do rozdziału aktywnego składnika (kwas usninowy) o właściwościach antybiotycznych z porostu Usnea barbata L. Ekstrakcję kwasu usninowego za pomocą CO2 w stanie nadkrytycznym z porostu Usnea barbata L. prowadzono w następujących warunkach: ciśnienie - 330 barów, czas ekstrakcji - 3 godz., temepratura - 40, 50, 60 i 80řC. Równocześnie dla porównania prowadzono klasyczną ekstrakcję za pomocą etanolu. Ponadto określono aktywność antybiotyczną kwasu usninowego otrzymanego za pomocą ekstrakcji CO2 w stanie nadkrytycznym wobec następujących drobnoustrojów: Staphylococcus aureus, Streptococcus pyogenes, Pneumococcus sp., Bacillus diphtheriae, Bacillus pumilus i Sarcina lutea.

Streszczenie angielskie: In the frame of this materials in use non-conventional extraction treatment for separation of active component - (usnic acid), natural antibiotic from lichen Usnea barbata L. Extraction of usnic acid with supercritical CO2 from lichen Usnea barbata L., was leading under following conditions: working pressure 300 bars, time of extraction - 3h, workind temperature 40, 50, 60 and 80řC. In the same time is confirmed efficiency and superiority of supercritical extraction with CO2, against to classic solid-liquid extraxtion with ethanol. There was researching antibiotics activity of usnic acid obtains with supercritical extraction with CO2 over following microorganisms: Staphylococcus aureus, Streptococcus pyogenes, Pneumococcus sp., Bacillus diphtheriae, Bacillus pumils and Sarcina lutea.

977/2523

Tytuł oryginału: Longitudinal changes in mandibular bone mineral density compared with hip bone mineral density and quantitative ultrasound at calcaneus and hand phalanges.
Autorzy: Drozdowska B., Pluskiewicz W.
Źródło: Br. J. Radiol. 2002: 75 (897) s.743-747, tab., bibliogr. 13 poz.
Sygnatura GBL: ,300,256

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of this prospective study was to evaluate changes in bone mineral density (BMD) of the mandible (m-BMD) compared with hip BMD and ultrasound parameters of the calcaneus and hand phalanges over 28 months. The study group consisted of 18 post-menopausal edentulous women with no reasons known to effect bone metabolism, such as disease or medication. Each women had undergone natural menopause. No previous fractures were noted. Measurements were performed at baseline and repeated after 28 months. Bone status was assessed by measuring BMD using dual energy X-ray absorptiometry (DXA) at the mandible and hip and by measuring speed of sound (SOS), broadband ultrasound attenuation (BUA), stiffness index (SI) and amplitude-dependent SOS (Ad-SOS) using quantitative ultrasound at the calcaneus and phalanges of the hand. The coefficient of variation for mandibular measurements was 2,6 p.c. BMD of the mandible, femoral neck and Ward's triangle decreased significantly (-7.54 p.c., -1.2 p.c., -1.97 p.c. per year, respectively, p 0.05), whilst BUA, SI and Ad-SOS decreased, but not significantly (-1.1 p.c., -0.47 p.c., -0.08 p.c. per year, respectively). Both SOS and BMD of the trochanter had almost the same value without significant differences. The least significant change (LSC), denoting the minimum difference between two successive results in an individual that can be considered to reflect a real change,was calculated. With the exception of changes in m-BMD, no significant changes were observed at any of the sites for the majority of the women (38-39 p.c.)...

978/2523

Tytuł oryginału: Tissue factor (TF) and tissue factor pathway inhibitor (TFPI) in the placenta and myometrium.
Autorzy: Kuczyński Jarosław, Uszyński Waldemar, Żekanowska Ewa, Soszka Tomasz, Uszyński Mieczysław
Źródło: Eur. J. Obstet. Gynecol. 2002: 105 (1) s.15-19, il., bibliogr. 17 poz.
Sygnatura GBL: 305,582

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Placental abruption is mainly due to the rupture of the uterine spiral arteries, but adjacent tissues are also damaged and substances derived from distintegrated placenta and myometrium probably cause coagulation disturbances, such as hypofibrinogenemia and disseminated intravascular coagulation (DIC). Aim: To measure whether placenta and myometrium are significant sources of tissue factor (TF) and tissue pathway inhibitor (TFPI), the components of key role in the initiation of coagulation. Materials and methods: Strips of placenta and myometrium were obtained from 48 women delivered by cesarean section at term. TF and TFPI concentrations were measured by immunoenzymatic method (ELISA) in tissue homogenates. Main results: Concentration of TF in placenta and myometrium was 31 (25-37) and 7.4 (4.9 - 11.4) ng/mg of total protein, respectively. In the plasma of the same women, it was 0.004 ng/mg of total protein. TFPI concentration was 13 (10 - 17) ng/mg of total protein in placenta, 4.5 (3.1 - 7.3) ng/mg in myometrium, and 0.70 (0.50 - 0.80) ng/mg in blood plasma protein. Conclusion: The levels of TF in placenta and myometrium, over TF in blood plasma may be clinically significant in obstetrics, for instance, in the etiology of DIC in placental abruption. The concentration of TFPI - natural inhibitor of TF - seems to be too low to prevent initiation of coagulation.

979/2523

Tytuł oryginału: Detection of serum Pneumocystis carinii DNA in the course of P. carinii pneumonia in experimental animals and HIV-infected patients.
Autorzy: Gołąb Elżbieta, Sobolewska Alicja, Bitkowska Elżbieta, Bednarska Agnieszka, Berak Hanna, Dzbeński Tadeusz H.
Źródło: Acta Parasitol. 2002: 47 (3) s.235-238, tab., bibliogr. [9] poz.
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: A nested PCR which amplified a part of the mitochondrial gene encoding the large subunit of rRNA of Pneumocystis carinii was used to detect P. carinii DNA in sera obtained from HIV-infected patients and immunosuppressed rats. P. carinii DNA was found in sera from nearly 52 p.c. of rats between 6th and 9th week of immunosuppressive treatment and in 12 serum samples out of 51 collected from HIV-infected patients, including 4 from clinically suspected and laboratory confirmed cases of PCP. The results of the studies indicated that DNA of P. carinii is most likely to appear in the blood in the advanced infection, however, the test for serum DNA may be of little value for the diagnosis of PCP since negative results do not exlude active infections and positive ones may be encountered in asymptomatic carriers.

980/2523

Tytuł oryginału: Pathogenesis of experimental toxoplasmosis in mice with strains differing in virulence.
Autorzy: Kodym Petr, Blażek Karel, Maly Marek, Hrda Stepanka
Źródło: Acta Parasitol. 2002: 47 (3) s.239-248, il., tab., bibliogr. s. 247-248
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Parameters of virulence and the course of infection were compared in mice infected with Toxoplasma gondii strains P-Cz (mice virulent), 38 (cyst-forming, highly pathogenic) and HIF (cyst-forming, virulent). Mice, that succumbed to infection with cyst-forming strain 38 had lesions in the spleen (days 7-14 p.i.), liver (days 7-14 p.i.), lungs (days 9-21 p.i.), heart (days 14-21 p.i.), and brain (days 21-35 p.i.). In contrast, the animals which succumbed to strain P-Cz had practically unaffected hearts and brains. There were no fundamental differences in the temporal sequence of histopathological changes in infection with virulent and both cyst-forming strains. Depending on the virulence of each strain the infections differed markedly in the degree of organ damage, duration of parasitemia, numbers of cysts in the brain , as well as in mortality and body weight loss. The differencesin post mortem findings are not due to different organ affinity, but to the virulence-dependent time of death. All P-Cc infected mice die in the early stage of infection, before their hearts and brains can be seriously damaged.

981/2523

Tytuł oryginału: Larval toxocarosis in mice: immunoreactivity after multiple high dose infections.
Autorzy: Aldawek Ahmed M., Levkut Mikulas, Revajowa Viera, Dvoroznakova Emilia
Źródło: Acta Parasitol. 2002: 47 (3) s.249-254, il., bibliogr. [24] poz.
Sygnatura GBL: 303,018

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Immunological responses of mice to threefold infection with infective Toxocara canis were studied up to 42 days. Mice of the experimental group were infected orally with 1000 infective eggs on days 0, 12, and 20 of the experiment (a total of 3000 eggs/mouse). The proliferative activity of spleen lymphocytes, production of specific antibodies in the serum, leukocyte counts, and T cells subpopulations in the blood and spleens were examined. The spleen T cells were significantly inhibited to 21 day post infection (dpi) while B cells were stimulated. An increased. An increased level of specific antibodies persisted almost the whole experiment. Leukocytosis with a dominant lymphocytosis was evident. In the peripheral blood the number of both CD8+ and CD4+ cells was significantly increased. Contradictory, a significant increase of the spleen CD8+ cells with a significant decrease of CD4+ cells was observed. In conclusion, the multiple high infection resulted in variable changes in the immune effector mechanisms. Some components were stimulated while others were inhibited which may suggest that immunosuppression is not the only major outcome of larval toxocarosis.

982/2523

Tytuł oryginału: Allergen-specific T lymphocytes as targets for specific immunotherapy: striking at the roots of type I allergy.
Autorzy: Bohle Barbara
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.233-241, bibliogr. 80 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In the past decades allergic diseases have tremendously increased and hypersensitivity reaction represent a growing healt concern in industrialized countries. Despite various effective therapeutic options for the treatment of allergic disease, only specific immunotherapy (SIT) has been shown to have effects on the underlying immunological mechanisms, namely functional changes at the level of T helper (Th) lymphocytes. It was found that allergen-specific CD4+ Th2 lymphocytes play a key in the pathophysiology of atopic disease. During successful SIT, the Th2-dominated immune response is modified towards a Th1 response, leading to a decline in allergen-specific IgE levels in the long term. In order to improve the efficacy and safety of SIT, novel approaches were developed targeting allergen-specific Th2 lymphocytes since specific inactivation or modulation towards Th1 cells could interfere with the disease process. In view of this aspect, this review will basically focus on two new, promising approaches to improve SIT: (1) the use of hypoallergenic proteins characterized by reduced IgE-binding capacities but retained T lymphocyte-activiting properties and (2) oligodeoxynucleotides containing CpG motifs as an example of adjuvants which foster Th1 immune responses. Both approaches promise to be capable of adjusting the pathological Th2 immune response.

983/2523

Tytuł oryginału: Type I interferons as immunoregulatory molecules; implications for therapy in experimental autoimmune uveoretinitis.
Autorzy: Mizuguchi Junichiro, Takeuchi Masaru, Usui Masahiko
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.243-254, bibliogr. 125 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Clinical trials have shown that type I interferon (IFN)-ŕ/á have some beneficial effects on organ-specific autoimmune diseases, such as Behcet's diseases and multiple sclerosis, although the precise mechanisms remain largely unresolved. T helper cells 1(Th1)-mediated autoimmune responses are involved in the initiation and/or progression of human uveitis, such as Behcet's disease. The animal model of experimental autoimmune uveoretinitis (EAU), characterized by a monophasic clinical course, has contributed to the understanding of the pathogenesis of human uveitis. Th1 producing IFN-ç induce EAU development, while Th2 producing IL-4/IL-10 prevent the disease. However, depending on the cytokine milieu, the pro-inflammatory cytokine IFN-ç may attenuate the autoimmune responses and anti-inflammatory cytokine IL-4 exacerbates it. Chemokines also play a crucial role in EAU development, which might be resolved by Th2-mediated immune responses. The administration of IFN-ŕ/á prevents EAU development, accompanied by a diminished production of IFN-ç/IL-10. Interestingly, however, IFN-ŕ/á also have some beneficial effects on patients with Th2-like phenotype in addition to Th1-like phenotype. Thus, the immuno-modulatory action of IFN-ŕ/á may be dependent on the context of cytokine combinant and/or their concentrations.

984/2523

Tytuł oryginału: V gene replacement in T and B lymphocytes: illicit or regimented rearrangement?
Autorzy: Golub Rachel
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.255-262, il., bibliogr. 60 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Lymphocytes can undergo novel types of secondary genetic rearrangements that after the specificity of a pre-existing B cell receptor (BCR) or T cell receptor (TCR). V gene replacement is one of them and it replaces an already rearranged V gene segment with an upstream germline V gene segment. This review focuses on the molecular aspect of rearrangement. The role of this mechanism in the immune system is debated.

985/2523

Tytuł oryginału: The TCR/CD3 complex: molecular interactions in a changing structure.
Autorzy: Feito Maria Jos, Jimnez-Perianez Arturo, Ojeda Gloria, Sanchez Alejandra, Portols Pilar, Rojo Jos M.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.263-272, il., bibliogr. 94 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The T cell receptor-CD3 (TCR/CD3) complex is a multichain structure in charge of antigen recognition in T cells. Despite many genetic, structural, and functional data obtained in recent years, essential questions concerning the TCR/CD3 complex still remain open, including: 1) the precise number of polypeptides in each TCR/CD3 complex, their interactions and spatial arrangement, 2) the role(s) of each polypeptide in antigen recognition and/or in receptor signal transmission, and 3) the relationship between the TCR/CD3 complex and other membrane or cytoplasmic molecules involved in downstream signaling. In this work we shall review data concerning some of these issues, proposing a model of the overall structure of the TCR/CD3 complex to explain its known features.

986/2523

Tytuł oryginału: DNA viruses and genetic modification of dendritic cells.
Autorzy: Jenne Lars, Steinkasserer Alexander
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.273-277, bibliogr. 34 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: An alternative approach to the use of tumor-peptide-loaded dendritic cells (DC) in immunotherapy would be the use of genetically modified DC using viral vectors expressing tumor-associated antigens (TAA). However, viruses have developed several immune escape mechanisms and, thus, one has to study the interaction between viruses and DC beforer these viruses can be used as an alternative strategy. Here we report that vaccinia virus (VV) as well as herpes simplex virus 1 (HSV-1) are able to potently infect monocyte-derived DC, however, this infection leads to the inhibition of the DC-mediated T cell stimulation in vitro.

987/2523

Tytuł oryginału: The emerging distinct role of TNF-receptor 2 (p80) signaling in chronic inflammatory disorders.
Autorzy: Holtmann Martin H., Schuchmann Marcus, Zeller Geraldine, Galle Peter R., Neurath Markus F.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.270-288, il., bibliogr. 95 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Tumor necrosis factor ŕ (TNF-ŕ) is a pleiotropic cytokine with strong proinflammatory and immunomodulatory properties. TNF-ŕ plays a critical role in many acute or chronic inflammatory diseases and anti-TNF strategies have proven to be clinically effective. Two TNF-specific cell surface receptors. TNF-R1 (p60) and TNF-R2 (p80), have been identified and the function of these receptors and the downstream intracellular signal-transduction pathways have been extensively studied in vitro. For a long time p60 was considered to be the predominant mediator of TNF signaling, whereas p80 was ascribed only an auxilliary function. However, there is increasing clinical and experimental evidence for an important independent role of p80 signaling in chronic inflammatory conditions. To date, most data exist for Crohn's disease. Upregulation of p80 and increased p80 signaling aggravates experimental colitis and is likely to contribute to the chronicity of inflammation in vivo. Further studies are required to elucidate critically important steps in TNF signaling that might be dysregulated. This will lead to a better understanding of the pathogenesis of these diseases and potentially reveal new, more specific therapeutic targets.

988/2523

Tytuł oryginału: The cGMP syntheisis and PKG1 expression in murine lymphoid organs.
Autorzy: Kurowska Ewa, Kobiałka Marcin, Zioło Ewa, Strządała Leon, Gorczyca Wojciech A.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (4) s.289-294, il., bibliogr. 35 poz.
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Numerous reports indicate that cyclic 3', 5' guanosine monophosphate (cGMP) is involved in the regulation of immune processes. However, the mechanisms responsible for the synthesis of this nucleotide and its signaling pathways in immune cells are still not well recognized. The aim of our studies was to establish: 1) which form of guanylyl cyclase (GC) synthesizes cGMP in murine lymphoid organs and 2) whether the same organs express the isoforms PKG1ŕ and/or PKG1á of protein kinase G, known as possible target for synthesized cGMP. Cells isolated from thymus, lymph nodes, and spleen were treated with activators (SNP, ANP, CNP, STa) of soluble or particulate cyclases. Sodium nitroprusside (SNP) elevated intracellular cGMP 2-fold in thymic and lymph node cells and about 10-fold in spleen cells. Atrial natriuretic peptide (ANP) caused modest but statistically significant increases of cGMP in cells of all three organs. Additionally, spleen cells elevated their cGMP content about 2-fold in response to C-type natriuretic protein (CNP). In cellular homogenates of the all analyzed organs, the antibody anti-PKG1á stained the 78 kDa band corresponding to the molecular mass PKG1. Only homogenates of spleen cells were stained by the antibody recognizing PKG1ŕ. Our results indicate that in the investigated organs cGMP may be synthesized mainly by soluble GC in response to nitric oxide. The modest increase of cGMP upon stimulation by ANP suggests that in all these organs either exists only a small subpopulation of cells that express particulate cyclase GC-A or GC-A is expressed at very low level...

989/2523

Tytuł oryginału: Chlorpromazine inhibits the glucocorticoid receptor-mediated gene transcription in a calcium-dependent manner.
Autorzy: Basta-Kaim A., Budziszewska B., Jaworska-Feil L., Tetich M., Leśkiewicz M., Kubera M., Lasoń W.
Źródło: Neuropharmacology 2002: 43 (6) s.1035-1043, il., bibliogr. [36] poz.
Sygnatura GBL: 305,141

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Antipsychotic drugs can modulate transcription factors ansd also nuclear receptors, but their action on glucocorticoid receptors (GR)-members of the steroid/thyroid hormone receptor family has been studied so far. In the present study we investigated effects of various antipsychotics on the glucocorticol-mediated gene transcription in fibroplast cells, stably tranfected with a mouse mammary tumor virus promoter (LMCAT cells). Chlorpromazine (3-100 ćM) inhibited the corticosterone-induced gene transcription in a concentration- and time-dependent manner. Clozapine showed a similar, but less potent effect, while haloperiod acted only in high concentrations, and other antipsychotics drugs (sulpiride, raclopride, remoxipride) were without any effect. It was also found that a phorbol ester (an activator of protein kinase C (PKC) and A-23187 (Ca2+-ionophore) attenuated the inhibitory effect of chloropromazine on the GR-induced gene transcription. An antagonist of the L-type Ca2+ channel, as an inhibitor of phospholipase C (PLC) inhibited the corticosterone-induced gene transcription, but had no effect on the chlorpromazine-induced changes. The involvement of a PKC/LC pathway in the chlorpromazine action was confirmed by Western blot analysis showed that the drug in question decreased the PLC-á1 protein level, and to a lesser extent that of the PKC-ŕ protein in LMCAT cells. The aferomentioned data suggest that inhibition of the glucorticosteroid-induced gene transcription by chlorpromazine and clozapine may be a mechanism by which these drugs block some effects induced...

990/2523

Tytuł oryginału: Biocompatibility of C-C composites covered with PyC and pHEMA.
Autorzy: Buresova M., Balik K., Machovic V., Novotna M., Pesakova V., Hulejova H., Sochor M.
Źródło: Inż. Biomater. 2002: 5 (20) s.3-5, il., tab., bibliogr. 3 poz.
Sygnatura GBL: 313,500

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

991/2523

Tytuł oryginału: Keratinolytic fungi in an acidic petroleum waste lagoon at a petroleum refinery.
Tytuł polski: Grzyby keratynolityczne w lagunie kwaśnych odpadów rafineryjnych na terenie rafinerii ropy naftowej.
Autorzy: Ulfig Krzysztof, Płaza Grażyna, Terakowski Maciej, Worsztynowicz Adam
Źródło: Rocz. Państ. Zakł. Hig. 2002: 53 (3) s.267-276, tab., bibliogr. 21 poz., sum.
Sygnatura GBL: 301,906

Hasła klasyfikacyjne GBL:

ochrona środowiska

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Celem pracy było przebadanie grzybów keratynolitycznych w lagunie kwaśnych odpadów rafineryjnych (przed bioremediacją) w obrębie rafinerii ropy naftowej znajdującej się na terenie gęsto zaludnionym. Wysiokie stężenia węglowodorów ropopochodnych (nie polarnych i polarnych związków alifatycznych oraz WWA) uniemożliwiły wzrost badanych grzbów w glinie i odpadach z laguny. Natruralny proces samooczyszczania znacznie obniżył zawartość węglowodorów, podwyższył pH i spowodował obfity wzrost Trichophyton ajelloi w glebie organicznej. Gleba ta zawierała materiał pochodzący ze strefy korzeniowej traw rosnących w kilku zielonych oazach na obrzeżu laguny. Nie można wykluczyć, że szczepy Trichophyton ajelloi zaadaptowane do skrajnych warunków w lagunie brały aktywny udział w procesie usuwania zanieczyszczeń ropopochodnych. Gatunki grzybów wyizolowane w niniejszej pracy nie stwarzaly istotnego zagrożenia epidemiologicznego. Zanieczyszczenia ropopochodne mogą jednak sprzyjać wzrostowi potencjalnych patogenów, np. Microsporum gypseum w innych typach gleb.

992/2523

Tytuł oryginału: Adhesion molecules of immunoglobulin gene superfamily in stroke.
Autorzy: Zaremba Jarosław, Losy Jacek
Źródło: Folia Morphol. 2002: 61 (1) s.1-6, bibliogr. 60 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Stroke-induced inflammatory reaction leads to the accumulation of leukocytes in the brain ischaemic region, where they exert a detrimental effect - promotion and extension of cerebral damage. Intracerebral infiltration of peripheral blood leukocytes requires prior endothelial-leukocyte interactions that are mediated by such cell surface proteins as adhesion molecules. Among adhesion molecules, it is the immunoglobulin gene superfamily (IgSF) that is responsible for strong attachment and transendothelial migration of leukocytes. The principal members of IgSF are: intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1). In this review the following issues were described and discussed: an increased expression of ICAM-1 and VCAM_1 in ischaemic brain as well as a detection of their soluble(s) forms in sera of stroke victims. The presented data suggest the involvement of both ICAM-1 and VCAM-1 in the sequence and timing of the infiltration of leukocytes into the brain ischaemic zone after stroke. They have also revealed changes in serum concentrations of sICAM-1 and sVCAM-1 that are characteristic for stroke. Recently, increase in sPECAM-1 levels in serum and cerebbrospinal fluid (CSF) has been shown within 24 h of the onset of stroke, having indirectly suggested involvement of the molecule in the inflammatory events during the early phase of stroke.

993/2523

Tytuł oryginału: Cholinergic innervation and calretinin-immunoreactive neurones in the hippocampus during postnatal development of the rat brain.
Autorzy: Ludkiewicz Beata, Wójcik Sławomir, Spodnik Edyta, Domaradzka-Pytel Beata, Moryś Janusz
Źródło: Folia Morphol. 2002: 61 (1) s.7-14, il., bibliogr. 41 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Immunohistochemical study of the cholinergic innervation of the hippocampal calretinin-containing cells was conducted on 28 rat brains of postnatal ages: PO, P4, P7, P14, P21, P30 and P60. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and calretinin were analysed using confocal laser-scanning microscope. Obtained data demonstrate that during development as well as in adult species calretinin-containing neurones in teh rat hippocampus form sparse synaptic contact with VAChT-ir terminals. It seems probable that cholinergic innervation is not crucial for the functioning of CR-ir cells - probably they remain under the greater influence of a system other than the cholinergic system.

994/2523

Tytuł oryginału: Uterus-innervating neurones of paracervical ganglion in the pig: immunochistochemical characteristics.
Autorzy: Wąsowicz Krzysztof, Podlasz Piotr, Czaja Krzysztof, Łakomy Mirosław
Źródło: Folia Morphol. 2002: 61 (1) s.15-20, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

weterynaria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnie

Streszczenie angielskie: Immunohistochemical characteristics of neurones innervating the porcine uterus located in paracervical ganglia were studied with a combination of retrograde fluorescent tracing and immunofluorescence. Retrograde fluorescent tracer Fast Blue (FB) was injected into the uterine horn and uterine cervix. The presence of biologically active substances, tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), galanin (GAL), Met-enkephalin-Arg-Gly-Leu (MEAGL) and calcitonin gene-related peptide (CGRP) was studied in FB-positive neurones localised in paracervical ganglia. FB-positive neurones containing TH, NPY, VIP and MEAGL were numerous, while those containing CGRP were scare. The results pointed to some species-related differences in immunochistochemical coding of neurones of paracervical ganglion responsible for uterus innervation.

995/2523

Tytuł oryginału: The morphological types of neurones of the medial and lateral mamillary nuclei in a newborn guinea pig: Nissl, Klver-Barrera and Golgi studies.
Autorzy: Robak Anna
Źródło: Folia Morphol. 2002: 61 (1) s.21-30, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Świnki morskie

Streszczenie angielskie: The studies were carried out on the hypothalamus of 5 newborn (PO stage) guinea pigs. The sections were impregnated according to three modifications of the Golgi technique or stained according to the Nissl and Klver-Barrera methods. On the basis of the shape and size of perikarya, dendroarchitecture, patern of axon as well as the inner structure of neurones, in the medial (Mm) and lateral (MI) mamillary nuclei for morphological types of nerve cells were distinguished: cap-like with two subtypes (33 p.c. of the cell population), fusiform (35 p.c.), triangular (12 p.c.) and rounded unidendritic (21 p.c.) neurones. The majority of them possessed spines on their dendrites. They spiny cells, both cap-like and fusiform ones, were observed preponderantly, in the medial mamillary nucleus, whereas in the lateral mamillary nucleus there were mainly seen the triangular and fusiform neurones, either spiny or aspiny cells. The spineless rounded unidendritic cells were dispersed throughout the mamllary region, but the were twice as numerous in Mm as in MI, where they were the least numerous.

996/2523

Tytuł oryginału: Development of valves in the small saphenous vein in human fetuses.
Autorzy: Czarniawska-Grzesińska Małgorzata, Bruska Małgorzata
Źródło: Folia Morphol. 2002: 61 (1) s.37-42, il., bibliogr. 15 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The study was performed on 82 small saphenous veins in human fetuses of both sexes aged 9 to 37 weeks. The earliest valves were observed in 13 week-old fetuses. In fetuses between 13 and 18 weeks old the number of valves increased from 1 to 8. In the older fetuses between 19 and 37 weeks the number of valves varied from 5 to 9 and does not seem to be relsated to age. During development more valves were found in the upper part of the small saphenous vein. The height of valves increases with age and differs between particular valves of the same vein.

997/2523

Tytuł oryginału: Multiple vessel variations in the retropubic region.
Autorzy: Sarikcioglu Levent, Sindel Muzaffer
Źródło: Folia Morphol. 2002: 61 (1) s.43-45, il., bibliogr. 9 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: We encountered some multiple vessel variationsin the retropubic region of a 55-year-old male cadaver. The obturator artery had its origin from the external iliac artery, and inferior epigastric artery from the femoral artery. Additionally, an anastomosis between obturator and inferior epigastric veins (venous Crown of death) was observed.

998/2523

Tytuł oryginału: Bilio-pancreatic anomalies obscured with MRCP.
Autorzy: Bobek-Billewicz Barbara, Gorycki Tomasz, Studniarek Michał, Szurowska Edyta
Źródło: Folia Morphol. 2002: 61 (1) s.47-51, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In this article the authors discuss whether or not diagnostic potential of MR cholangiopancreatography is strong enough to replace direct cholangiography in all cases. The pre- surgery analysis of a variety of pancreato-biliary disorders diagnosed using MRCP images is presented with the emphasising the importance of source images. Six cases of pancreato-biliary disorders are presented in which MRCP indicated the place of ductal stenosis as well as the morphologic variants or ductal uninspected shape which is critical for surgery or planned drainage. Coronal and axial MRCP source and MIP images were obtained with 0,5T Gyroscan NT. Anomalies of the biliary or pancreatic ducts included two cases of choledochal cystic dilatation; two cases of aberrant biliary ducts, one case of gallbladder duct variant and a case of an additional pancreatic duct. In 3 out of 6 cases, the MRCP source images produced using the complementary method supplied more complete information concerning ductal junctions than teh MIP images. Whereas in 3 out of 6 cases, both kinds of images were equally reliable. In 4 out of 6 cases, endoscopy was performed, an din 2 cases ERCP images were not diagnostic for ductal anatomy. However, full delineation of biliary and pancreatic ducts was complete in all MRCP images. MRCP within source images and maximum intensity projections show particular promise for the assessment of pancreatobiliary anomalies in order to reduce the number of higher-risk endoscopic interventions. The technique should be the method ...

999/2523

Tytuł oryginału: Aberrant left subclavian artery.
Autorzy: Jakubowicz Marian, Ratajczak Wojciech, Nowik Marek
Źródło: Folia Morphol. 2002: 61 (1) s.53-56, il., bibliogr. 20 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: This paper describes a rare case in which the left subclavian artery originates from a common stem arising from the aortic arch and splits into a brachiocephalic trunk and a left subclavian artery. The course of other large vessels of the aortic arch in this case are typical.

1000/2523

Tytuł oryginału: Neurological symptoms as the result of enlarged dimensions and non-typical course of inferior superficial temporal vein.
Autorzy: Maciejewski Ryszard, Drop Andrzej
Źródło: Folia Morphol. 2002: 61 (1) s.57-60, il., bibliogr. 20 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Neurological symptoms as the result of non-typical course of superficial cerebral veins are described in available literature very rarely. The case described below indicates that in some circumstances the compression symptoms derived from the cerebral cortex may be incredibly more serious than their anatomical reasons. In our observation a young woman was described complaining of paroxysmal numbness of the left upper limb with paraesthesiae of the left side of the face, the left eye and left half of the tongue. The patient said that in childhood she used to have paroxysmal itching of the left hand. She also said that CT of the head made a few years ago after a car accident was without pathological changes. Neurological examination, x-ray of the skull and EEG test performed during first visit proved normal. After one year of the disease, Jackson-type epilepsy, combined with loss of sensation of the left half of the face for the firszt time, was present. Neurological andx ophthalmological examination of the bottom of the eye proved normal. Skull x-ray was normal. Then disturbances of the vision in the left half of the field appeared. EEG was still in norm. The MRI the showed the asymmetry in the course and dilated superficial vein between teh basis of the right temporal lobe and the tentorium of the cerebellum. The diameter of this vein was 2.5 mm, but there were no vascular malformations. Bottom of the eye normal, but in the field of vision the white and red colours were dominated.

1001/2523

Tytuł oryginału: Professor Władysław Kubik (1923-2000).
Autorzy: Narkiewicz Olgierd, Grzybiak Marek
Źródło: Folia Morphol. 2002: 61 (1) s.61-62, il.
Sygnatura GBL: 301,720

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Kubik

Władysław 1923-2000

1002/2523

Tytuł oryginału: Visualisation of diverticula of the upper part of the alimentary tract; comparison of roentgenologic and endoscopic techniques.
Autorzy: Burdan Franciszek, Różyło-Kalinowska Ingrid, Juśkiewicz Wit, Maciejewski Ryszard, Wallner Grzegorz, Zgodziński Witold, Zinkiewicz Krzysztof, Złomaniec Janusz, Dąbrowski Andrzej
Źródło: Folia Morphol. 2002: 61 (1) s.297-301, il., bibliogr. 37 poz.
Sygnatura GBL: 301,720

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Diverticula of the upper part of the alimentary tract, irrespcetive of their etiology, are frequently observed benign changes of the pharynx, oesophagus, stomach and duodenum. In the present work, patients of the II General Surgery Department of the Medical University of Lublin, with radiologiaclly or endoscopically proved diverticula of the upper part of the alimentary tract, were examined. The presence of diverticula of such localalisation was an indication for supplementary endoscopic or radiological examination. The localisation, size, diameter of the opening, mucosal relief of diverticula and its contiguity were checked and analysed. Our data suggest that both medical procedures are complementary to each other. All previously observed changes in diverticula of the thoracic part of the oesophagus and the infradiaphgram part of the alimentary tract were fully proved. The radiological examination gave a better view of Zenker's diverticulum, especially in short and obese patients. Sampling and better visualisation of the diverticula opening testify to the unquestionable superiority of endosopy. However, precise evaluation by radiological process fully completes the diagnostic protocol. Both diagnostic procedures are usually supplemented by manometric examination of the oesophagus and superior and inferior oesophageal sphincters. This enables teh accurate diverticulaetiology to be stated.

1003/2523

Tytuł oryginału: Professor Stanisław Misztal, (1927 - 2002).
Autorzy: Przegaliński Edmund
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.211-213, il.
Sygnatura GBL: 313,156

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Misztal

Stanisław 1927-2002

1004/2523

Tytuł oryginału: Beneficial and harmful effects of thiols.
Autorzy: Włodek Lidia
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.215-223, il., bibliogr. 47 poz.
Sygnatura GBL: 313,156

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Biothiols are extraordinarily efficient antioxidants protecting the cells against conseuences of damage induced by free radical, due to their ability to react with the latter. In such antioxidant reactions, thiols undergo oneelectron oxidation with the formation of thiyl radicals. For this reason, attention has been focused mostly on antioxidant properties of thiols. Considerably less attention has been paid to thiyl radicals (RS) formed simultaneously in these reactions. However, protective and repairing efficacy of thiols depends not only on their capacity to detoxify free radicals but also on chemical character and reactivity of the formed thiyl radical. Furthermore, quick and efficient removal of RS radical leads to disturbance in balanced state of antioxidant reaction, which effectively increases repairing capacity. Dangerous thiyl radicals, which can cause peroxidative injury, should immediately undergo regenerative reduction to thiols. Under physiological conditions, thiyl radicals can react with thiolate anion yielding disulfide radical anion (RSSR) as an intermediate and finally disulfides and superoxide radical anion (O2), which is next inactivated in teh reaction catalyzed by superoxide dismutase (SOD). Thiyl radicals can also be reduced to thiols by reacting with ascorbate with the formation of low-activity ascorbyl radical, that subsequently enters disproportiation reaction.

1005/2523

Tytuł oryginału: Neurotransmitter levels and [3H]muscimol binding sites in the brain of rats selectively bred for alcohol preference and non-preference.
Autorzy: Dyr Wanda, Siemiątkowski Marek, Krząścik Paweł, Bidziński Andrzej, Płaźnik Adam, Kostowski Wojciech
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.225-230, tab., bibliogr. 29 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Alcohol-naive high- (Warsaw High Preferring; WHP) and low- (Warsaw Low Preferring; WLP) preferring lines of rat were studied to determine the distribution and density of [3H]muscimol binding sites in the brain using quantitative autoradiography. The results have shown no difference in the density of [3H]muscimol binding sites in the cingulate and frontal cortex, dorsal and ventral striatum, lateral and medial septum, caduate-putamen, nucleus accumbens in the both lines of animals. In the separate experiments, the levels of neurotransmitters were measured in the frontal cortex, hippocampus and striatum of the WHP and WLP rats. The content of dopamine was significantly lower in the striatum of the WHP rats as compared to the WLP animals. The levels of serotonin and noradrenaline were without any important differences in the examined structures of both lines of rats.

1006/2523

Tytuł oryginału: Effects of ŕ-ketoglutarate on lipid peroxidation and antioxidant status during chronic ethanol administration in Wistar rats.
Autorzy: Velvizhi Selvaraj, Nagalashmi Thiyagarajan, Essa Mustafa Mohamed, Dakshayani Kadiyala Babu, Subramanian Perumal
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.231-236, tab., bibliogr. 35 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The effects of ŕ-ketoglutarate (ŕ-KG) on ethanol-induced hepatotoxicity were studied in biochemiacal experiments in rats. The levels of serum transaminases and thiobarbituric acid reactive substances were significantly increased in ethanol-treated rats. These levels were significantly decreased in ŕ-KG- and ethanol-treated rats. Further, non-enzymatic (vitamins C and E) and enzymatic (superoxide dismutase and catalase) antioxidants were significantly decreased in ethanol-treated rats, and were increased in ŕ-KG- and ethanol-treated rats. The biochemical alterations during ŕ-KG treatment could be due to (i) teh participation of ŕ-KG in the non-enzymatic oxidative decarboxylation in the hydrogen peroxide decomposition process and (ii) enhancing the proper metabolism of fats which could suppress oxygen radical generation and thus prevent the lipid peroxidative damages in rats.

1007/2523

Tytuł oryginału: Influence of SKF 91488, histamine n-methyltransferase inhibitor, on the central cardiovascular regulation during controlled, stepwise hemorrhagic hypotension in rats.
Autorzy: Jochem Jerzy, Żwirska-Korczala Krystyna, Rybus-Kalinowska Barbara, Jagodzińska Julia, Korzonek-Szlacheta Ilona
Źródło: Pol. J. Pharmacol. 2002: 54 (3) s.237-244, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The histamine system influences various activities of the central nervous system, including cardiovascular regulation. Histamine administered intracerebroventricularly (icv) in anesthetized rats produces the increase in mean arterial pressure (MAP) and xdheart rate (HR), however, in contrast to normotensive animals, histamine-induced rises in MAP an dHR in critically hypotensive animals are significantly higher. Similarly to exogenous histamine, inhibition of the central histamine N-methyltransfer (HNMT) activity (the enzyme catabolizing histamine in the central nervous system) resulting in the increase in endogenous histamine concentration, also leads to the pressor effect in normotensive rats. The present study was designed to determine the role of endogenous centarl histamine in cardiovascular regulation in a rat model of blood volume-blood pressure controlled hemorrhagic hypotension. In normotensive animals, HNMT inhibitor SKF 91488 produced dose-dependent (20-100 ćg icv) pressor effect accompanied by tachycardia, similarly as exogenous histamine (0.5-5 ćg icv) did. The subpressor dose of SKF 91488 (10 ćg) evoked the increased in blood volumes necessary to induce hypotension of 40 and 20 mmHg and the action was accompanied by the rise in histamine concentrations in the hypothalamus (5.18 ń 0.45 vs 4.23 ń 0.41 nmol/g; p 0.5) and medulla oblongata (0.41 ń 0.05 vs 0.30 ń 0.06 nmol/g; p 0.05), with no changes in the cortical histamine concentrations (0.84 ń 0.18 vs 0.75 ń 0.17 nmol/g), compared to the conrol icv saline-treated group...

1008/2523

Tytuł oryginału: The effect of poneratoxin on neuromuscular transmission in the rat diaphragm.
Autorzy: Hendrich Andrzej B., Mozrzymas Jerzy W., Konopińska Danuta, Scuka Maria
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.195-202, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The effect of the ant venom neuropeptide - poneratoxin (PoTX) - on neuromuscular transmission in rat diaphragm tissue was studied by means of intracellular recordings of spontaneous miniature endoplate potentials (MEPPs)and of nerve evoked endplate potentials (EPPs). A 2 ćM concentration of PoTX caused a pronounced but transient increase in MEPPs frequency. Moreover, within the first few minutes of PoTX administration, the area, rise time and half decay time of MEPPs gradually decreased, reaching steady values after 15-20 min. The alteration of the area, rise time and half decay time of EPPs after PoTX application was similar to that observed for MEPPs. We conclude that PoTX affects neuromuscular transmission in rat tissue, and suggest that PoTX could exert both pre- and postsynaptic effects.

1009/2523

Tytuł oryginału: W. Mejbaum - Katzenellenbogen's Molecular Biology Seminars. 9. Liposomes. From models to applications: abstracts.
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.213-305, il., tab., bibliogr. [przy streszcz.] - Seminarium pt. Liposomy od modeli do zastosowań Wrocław - Szklarska Poręba - Piechowice 26-29.05. 2002
Sygnatura GBL: 306,513

Typ dokumentu:

1010/2523

Tytuł oryginału: Hip biomechanics in orthopaedic clinical practice.
Autorzy: Antolic Vane, Kralj-Iglic Veronika, Iglic Ales, Pompe Borut
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.311-315, il., tab., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Mantuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Radiographic and clinical studies, cupled with biomechanical assessment of the hip, are important tools for predicting the development of hip osteoarthitis of the hip. In order to better understand the treatment of hip dysplasia, it is necessary to determine the contact stress in the hip joint. In this study, a three-dimensional mathematical model was used to determine hip joint contact stress. Because of the discrepancy in the results of analyses of different radiographic indicators of hip dysplasia, the calculation of hip joint contact stress is proposed for a more accurate assessment of the severity of hip dysplasia.

1011/2523

Tytuł oryginału: Ionic mechanisms involved in the nodal swelling of myelinated axons caused by marine toxins.
Autorzy: Benoit Evelyne, Mattei Cesar, Ouanounou Gilles, Meunier Frederica A., Suput Dusan, Le Gall Frederic, Marquais Michel, Dechraoui Marie Y., Molgo Jordi
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.317-321, bibliogr. 15 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

Żaby

Streszczenie angielskie: This review describes the ionic mechanisms involved in the nodal swelling of frog myelinated axons caused by specific marine neurotoxins (ciguatoxins, brevetoxins, Conus consors toxin and equinatoxin-II), analysed using confocal laser scanning microscopy. We have focussed on toxins that either target neuronal voltage-dependent Na+ channels, or that form cationselective pores and indirectly affect the functioning of the Na+-Ca++ exchanger.

1012/2523

Tytuł oryginału: Requirements for the localization of p130 Cas to Z-lines in cardiac myocytes.
Autorzy: Kovacic-Milivojevic Branka, Damsky Caroline C., Gardner David G., Ilić Dusko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.323-329, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The vertebrate heart responds to hemodynamic load with the enlargement of postmitotic, terminally differentiated cardiac myocytes. Such hypertrophic changers are characterized by alternations in sarcomeric organization and gene expression. Previously, we estabilished a role for a nonreceptor tyrosine kinase, focal adhesion kinase, in signaling the changes in cytoskeletal organization associated with hypertrophy. Here, we report on data suporting a key role for p130Cas in this process,. In neonatal cardiac myocytes FAK, Cas and paxillin are located in sarcomeric Z-lines, suggesting that the Z-line is an important signaling locus in these cells. The expression of different Cas mutants results in a nearly complete loss of sarcomeric organization in these myocytes. Moreover, expression of the C-terminal focal adhesion-targeting domain of FAK both disrupted sarcomeric organization and interfered with the localization of endogenous Cas to Z-lines. These findings suggest that the association of FAK and Cas and the preservation of multiple protein-interaction motifs of Cas are required for the correct assembly of sarcomeres in cardiac myocytes.

1013/2523

Tytuł oryginału: A paradigm for quantifying ventricular contraction.
Autorzy: Palladino Joseph L., Noordergraaf Abraham
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.331-335, il., tab., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The left ventricle may be described as a time, volume and flow dependent pressure generator. First, isovolumic pressure is measured at various and-diastolic volumes. Next, pressure is adjusted to account for small changes accompanying ejection, denoted the ejection effect. The resulting analytical function can describe pressure generation and ventricular outflow of the ventricle under a wide range of contractile and vascular conditions. This paradigm is unique in separating isvolumic from ejecting ventricular properties, as well as ventricular from vascular conditions.

1014/2523

Tytuł oryginału: A dosimetric method of total body irradiation.
Autorzy: Vrtar Mladen
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.337-340, il., bibliogr. 6 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The determination of the dose delivered to the body during Total Body Irradiation (TBI) is not easy, as direct measurements are impossible. This article presents the intention to develop a systematic and simple method for absorbed dose determination in 60Co TBI using mid plane dose factors (MDF) and external measurements, i.e. entrance and exit dose readings by semiconductor detectors. In order to perform the radiation field analysis prior to TBI treatment, a special silicon n-type detector with increased sensitivity (1200 nC/Gy) was used. The dependence of the calibration factor of this detector exposed to different dose rates in standard and TBI conditions was investigated. A theoretical model of anthropomorphic phantoms of cylindrical ellipsoid shape was estabilished. The software made it possible to generalize the applicability of TBI dosimetry to any individual case of real patient data. A quality assurance analysis of dosimetric results of 350 patients, who underwent TBI during a 17-year period, was performed.

1015/2523

Tytuł oryginału: Cell markers in the recognition of acute myeloblastic leukaemia subtypes.
Autorzy: Andoljsek Dusan, Preloznik Zupan Irena, Zontar Darja, Cernelc Peter, Mlakar Uros, Modic Mojca, Pretnar Joze, Zver Samo
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.343-345, tab., bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The diagnosis of acute myeloblastic leukaemia (AML) is based on cell morphology, cytogenetic and molecular changes, cell markers and clinical data. Our aim was to estabilish whether morphology and cell markers are comparable in the evaluation of AML. Bone marrow smears were analysed, and flow cytometry and monoclonal antibodies were used to determine cell type and maturity. Morphology and cell markers correlated differently in different AML subtypes.

1016/2523

Tytuł oryginału: Modelling the state of bone cysts by statistical analysis of intensities of X-ray images.
Autorzy: Brecelj Janez, Bole Velimir
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.347-349, il., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The subject of this article is the state of a benign bone disease. The principle aim of this study is the construction of a statistical model for estimating the otherwise unobservable variable for the state of the disease. The distribution of intensity of X-ray images of the affected part and the healthy part of the bone are evaluated. Quantiles of both distributions are used in the estimation of a multinomial logit model by which the variable for the state of the disease is quantified.

1017/2523

Tytuł oryginału: Equinatoxin II-induced lysis of the cultured endothelial cell line ECV-304.
Autorzy: Bunc Matjaz, Rozman Janez, Starc Radovan, Macek Peter, Suput Dusan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.351-353, il., tab., bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Equinatoxin II (EqT II) is a pore-forming actinoporin. Its lethality in rat tissue is due to cardio-respiratory effects. The toxin contracts the vascular smooth muscle only in the presence of intact endothelium. In our study, its effects on the endothelial cell culture ECV-304 were tested. The EqT II effects were dose-dependent and were influenced by calcium ions and sucrose. The obtained results support the conclusion that calcium ions are the intracellular messengers of the EqT II effects on the isolated endothelial cells.

1018/2523

Tytuł oryginału: Nicardipine diminished equinatoxin II-induced decrease of coronary flow in isolated rat and pig hearts.
Autorzy: Bunc Matjaz, Rozman Janez, Vidmar Alenka, Rakovec Peter, Drevensek Gorazd, Budihna Metka V., Suput Dusan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.355-357, bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Equination II (EqT II) is a basic, cardiotoxic polypeptide. The vasoconstrictory effect of the toxin on isolated porcine coronary arteries was diminished by nicardipine, an L-type calcium channel antagonist. A comparison was made of the effects of EqT II alone and EqT II in the presence of nicardipine on the coronary flow in porcine and rat hearts isolated according to Langendorff's method. In both models EqT II decreased coronary flow in a dose-dependent manner and there were no statistically significant differences between the two models (p 0.05). However, 1ćM nicardipine diminished the effects of EqT II on coronary flow in isolated porcine hearts more than in isolated rat hearts (p 0.05). The results suggest that the activation of L-type calcium channels is one of the mechanisms involved in the lowering of coronary flow induced by EqT II.

1019/2523

Tytuł oryginału: The importance of HLA DQB1*0602 typing in Slovene patinets with narcolepsy.
Autorzy: Dolenc-Grosel Leja, Vodusek David B.
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.359-360, tab., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The HLA class II region genes DQB1*0602 and DQA1*0102 are currently the best genetic predictors for narcolepsy in humans. The aim of this study was to identify the HLA DQ alleles (DQB1*0602 and DQA1*0102) in Slovene sporadic narcoleptic patients. 11 patients who fulfilled ICSD criteria for narcolepsy entered the study. DRB1*1501 DQB1*0602 was present in all the patients while DQA1*0102 was absent in 2 patients. We propose that DQB1*0602 typing is important in diagnosing narcolepsy in Slovene patients.

1020/2523

Tytuł oryginału: The outcome of autologous chondrocyte transplantation treatment of cartilage lesions in the knee.
Autorzy: Drobnic Matej, Kregar-Velikonja Nevenka, Radosavljevic Damjan, Gorensek Miro, Koritnik Branko, Malicev Elvira, Wozniak Gordana, Jeras Matjaz, Knezevic Miomir
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.361-363, il., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Recent results of the clinical outcome of autologous chondrocyte transplantation (ACT) treatment in a group of 28 patients with focal femoral condyle cartilage lesions revealed a correlation trend with the quality of the in vitro cell culture matrix-protein synthesis. No impact of the patients' age and chondrocyte cryopreservation prior to implantation was observed. Further studies are needed to confirm the preliminary results.

1021/2523

Tytuł oryginału: Biomechanical analysis of Chiari osteotomy.
Autorzy: Herman Simon, Kralj-Iglic Veronika, Iglic Ales, Antolic Vane, Herman Srecko
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.365-366, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

chirurgia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The decrease in contact hip joint stress after Chiari osteotomy is studied using a mathematical model. In the model, additional coverage of the femoral head by the ala ossis ilei segment is taken into account. It is shown that this additional coverage significantly decreases stress, mostly by the indirect effect caused by the shift of the stress pole.

1022/2523

Tytuł oryginału: The muscle adaptation process as a result of pathological changes or specific training procedures.
Autorzy: Kersevan Katarina, Valencic Vojko, Djordjevic Srdjan, Simunic Bostjan
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.367-369, il., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

medycyna sportowa

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Muscle responses to tetanic electrical stimulation were detected by the non-invasive tensiomyographic (TMG) measuring method. The main objective of this study was to find out whether the TMG measuring method is suitable for monitoring the unfused tetanus (stimulation frequencies ranging from 1 Hz to the fusion frequency (ff) - the frequency at which tetanus occurs), and whether this monitoring provides any information on skeletal muscles, structural or functional changes. The muscle adaptation process was observed in damped unfused tetanus (DUT). The measured results in the clinical environment as well as on the sports field indicate that DUT is caused by a type II muscle fibres fatigue process. Separate observation of type II muscle fibres enables more efficient treatment and observation of pathological changes, and helps professional athletes and their trainers to better understand the influence of training stimulii on the training process.

1023/2523

Tytuł oryginału: Echocardiographic evaluation of regional left atrial function after rapid atrial pacing.
Autorzy: Kozelj Mirta, Zupan Igor, Butinar Janos, Seliskar Alenka, Rakovec Peter
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.371-373, tab., bibliogr. 6 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

psy

Streszczenie angielskie: Rapid regular atrial pacing (RAP) produces changes in atrial function similar to those caused by atrial fibrillation in animal models. Left atrial appendage (LAA) function represents regional atrial function. The aim of our study was to investigate the influence of RAP on left atrial regional function and to evaluate the reversibility of changes after terminationtermination of pacing in a canine model. Eight dogs were subjected to RAP (400 bpm) for 16 days. Transesophageal echocardiography was performed at baseline, immediately after RAP and 4 weeks after the terminationtermination of RAP. The LAA peak late emptying velocity (LAA-E) and filling wave (LAA-f) were measured. LAA-E velocities were significantly reduced and filling wave velocities (LAA-f) were significantly less negative after RAP compared with the baselline values. Four weeks after terminationtermination of pacing, the LAA-E and LAA-f velocities were normal. RAP results in impaired regional atrial systolic and diastolic function. The changes were completely reversible 4 weeks after terminationtermination of pacing. These results suggest that the LAA is mechanically stunned after RAP.

1024/2523

Tytuł oryginału: Differences in the oxygenation of the forearm muscle during isometric contraction in trained and untrained subjects.
Autorzy: Usaj Anton
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.375-377, il., bibliogr. 2 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

medycyna sportowa

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of this study was to test a hypothesis that a longer duration of isometric contraction is related to an increased oxygenation status of the muscle. A group of trained rock climbers and untrained subjects performed 15 kp sustained isometric contraction until fatigue set in. The oxygenation status was assessed using near infrared spectroscopy (ISS, USA). The results support the hypothesis. The concentration of relative oxygenated hemoglobin was higher in the rock climbers than in the untrained subjects. The relative total hemoglobin did not differentiate the two groups enough to show that blood volume also strongly influences contraction time.

1025/2523

Tytuł oryginału: The effect of proprioceptive training in patients with recurrent dislocation of the patella.
Autorzy: Vengust Rok, Strojnik Vojko, Pavlovicic Vinko, Antolic Vane, Zupanc Oskar
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.379-380, il., bibliogr. 3 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Eleven patients with recurrent dislocation of the patella were subjected to knee proprioceptive training. Patients exhibited a gain in their Lysholm and Activity scores (p 0.03 and 0.009). No patient needed operative procedure.

1026/2523

Tytuł oryginału: Hazard identification for 3,5-dichlorophenol in the aquatic environment.
Autorzy: Zagorc-Koncan Jana, Zgajnar Gotvajn Andreja, Tisler Tatjana
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.381-382, bibliogr. 5 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

ochrona środowiska

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The risk assessment of substances in various environmental compartments is essential for their proper, safe and environmentally friendly production, handling, use and final deposition or destruction. Hazard identification is an important part of risk assessment. The aim of our research was to present a methodology for the hazard identifuication of substances dangerous to the aquatic environment according to the 93/21/EEC Directive relating to the classification, packaging and labelling of dangerous substances, from the adverse effect assessment of chemicals in European Union. A battery of toxicity tests and biodegradability studies with 3,5-dichlorophenol were performed. The substance was classified as toxic to aquatic organisms with possible long-term adverse effects. The presented methodology assures reliable data for the classification and labelling of substances according to their harmful effect on aquatic biota, suitable for the competent authorities at the national and EU level.

1027/2523

Tytuł oryginału: Impaired sinus node function and global atrial conduction time after high rate atrial pacing in dogs.
Autorzy: Zupan Igor, Kozelj Mirta, Butinar Janos, Rakovec Peter
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (2) s.383-384, tab., bibliogr. 4 poz. - Międzynarodowa konferencja pt. Nauki przyrodnicze Gozd Martuljek 22-26.09. 2001 - Cz. 1 mater. konferencyjnych zamieszczono w nr 1/2002
Sygnatura GBL: 306,513

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

psy

Streszczenie angielskie: In animal and human studies, it has been shown that atrial fibrillation shortens the atrial refractory period and impairs its rate adaptation. The objective of this study was to evaluate the effects of high-rate pacing on sinus node function and intra-atrial conduction. Eight dogs were subjected to rapid atrial pacing (AP) at 400 bpm for 16 days. Sinus node recovery time (SNRT) and P-wave duration were measured at baseline, immediately after AP and four weeks after the termination of AP. SNRT immediately after AP was significantly prolonged at all pacing rates compared to the baseline values. P-wave duration was significantly longer after AP relative to the baseline values. All the variables were completely reversible four after the termination of pacing. Rapid AP induces sinus node dysfunction and prolongs the intra-atrial conduction time. IT is possible that the electrical remodelling extends to the sinus node as well.

1028/2523

Tytuł oryginału: Neuronal basis of neuroleptic-induced extrapyramidal side effects.
Autorzy: Ossowska Krystyna
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.299-312, il., bibliogr. 80 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The article reviews presently commonly accepted concepts of neuronal basis of neuroleptic-induced extrapyramidal side effects. The data obtained both, in humans and laboratory animals, point to the blockade of a large number of the striatal dopamine D2 receptors by neuroleptics as a primary cause of these disturbances. This phenomenon leads to the appearance of parkinsonian symptoms shortly after therapy commencement. On the other hand, chronic administration of neuroleptics evokes supersensitivity to dopamine connected with the increased number of D2 receptors and supersensitivity of D1 receptors, which can be significant for the development of tardive dyskinesia. Primary and secondary changes in the function of dopamine receptors lead to partially opposite, pathological changes in the activity of neuronal pathways connecting the basal ganglia. Besides functional changes, neuroleptic-induced lesions of the striatal neurons and genetic predispositions can also play a role in tardive dyskinesia.

1029/2523

Tytuł oryginału: Neuroprotective role of adenosine in the CNS.
Autorzy: Wardas Jadwiga
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.313-326, il., tab., bibliogr. 117 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: It is well estabilished that in the CNS, endogenous adenosine plays a pivotal role in neurodegeneration. A low, nanomolar concentration of adenosine is normally present in the extracellular fluid, but it increases dramatically during enhanced nerve activity, hypoxia or ischemia. In these pathological conditions, adenosinergic transmission-potentiating agents, which elevate adenosine level by either inhibiting its degradation (adenosine deaminase and kinase inhibitors) or preventing its transport, offer protection against ischemic or excitotoxic neuronal damage. The directly acting adenosine A1 receptor agonists are known to mediate neuroprotection, mostly by the blockade of Ca2+ influx, which results in the inhibition of glutamate release and reduction of its excitatory effects at a postsynaptic level. More recent data have shown that antagonists of adenosine A2A receptors markedly reduce cerebral ischemic damage in animal models of focal and global ischemia. Moreover, these compounds attenuate the neuronal loss induced by excitatory amino acids (EAA). A neuroprotective effect of adenosine A2A receptor antagonists was also shown in animal models of Parkinson's disease (MPTP, 6-OHDA, methamphetamine). Hence, it might be suggested that adenosine A2A receptor antagonists may represent a novel strategy in the therapeutic approach to pathologies characterized by acute or chronic neurodegenerative events, since they not only reverse motor impairment but can act as neuroprotective compounds by promoting cell survival.

1030/2523

Tytuł oryginału: In the search for selective ligands of 5-HT5, 5-HT6 and 5-HT7 serotonin receptors.
Autorzy: Wesołowska Anna
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.327-341, il., tab., bibliogr. 65 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: In recent years much attention has been focused on the functional importance of 5-HT5, and 5-HT6 and 5-HT7 receptors in the pathogenesis of neuropsychiatric and other diseases. In this connection, intensive studies with ligands of these receptors are currently in progress. Recognition of the structural characteristics responsible for the bilding of a ligand molecule to an appropriate receptor, and development of an active complex have reached an advanced stage in the research for selective compounds. This review was undertaken to summarize the results of structure-activity relationship studies with ligands of 5-HT5, 5HT6 and 5-HT7 receptors. Additionally, some data on localization, pharmacological properties and the functional role of those receptors were reported.

1031/2523

Tytuł oryginału: Effect of antidepressant drugs on the hypothalamic-pituitary-adrenal axis activity and glucocorticoid receptor function.
Autorzy: Budziszewska Bogusława
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.343-349, bibliogr. 50 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Hyperactivity of hypothalamic-pituitary-adrenal (HPA) axis is the main biochemical change, besides disturbed monoaminergic neurotransmission, observed in the patients suffering from a major depression. High incidence of depression in Cushing's syndrome as well as antidepressant effects of adrenocortical enzyme inhibitors in major depression [17, 20, 29, 42] support hypothesis that hyperactivity of HPA axis may be involved in pathogenesis of depression.

1032/2523

Tytuł oryginału: Effect of intra-tegmental microinjections of 5-HT1B receptor ligands on the amphetamine-induced locomotor hyperactivity in rats.
Autorzy: Papla Iwona, Filip Małgorzata, Przegaliński Edmund
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.351-357, il., bibliogr. 35 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Previous research demonstrated that the mesoaccumbens dopamine (DA) pathway played a critical role in the behavioral effects of amphetamine in rodents. Nonetheless, recent findings have also indicated involvement of 5-hydroxytryptamine (5-HT, serotonin) transmission in these effects. In the present study, we investigated the role of 5-HT1B receptors located in the ventral tegmental area (VTA) in the amphetamine-induced locomotor hyperactivity in rats. Male Wistar rats, implanted bilaterally with cannulae in the VTA were infused with saline (0.2 ćl/side), GR 55562 (an antagonist of 5-HT1B receptors; 0.1 - 1 ćg/side) or CP 93129 (an agonist of 5-HT1B receptors; 0.003 - 0.03 ćg/side) immediately prior to the injection of saline (1 ml/kg, ip) or amphetamine (0.5 mg/kg, ip). The monitoring of locomotor activity in photobeam chambers began at once and proceeded for 60 min. Neither GR 55562 nor CP 93129 affected basal locomotor activity. Pretreatment with GR 55562 (0.1 - 1 ćg/side) did not affect the locomotor hyperactivity evoked by amphetamine. On the other hand, microinjections of CP 93129 (0.01 - 0.03 ćg/side) enhanced the amphetamine-induced hyperlocomotor activity. GR 55562 (1 ćg/side) markedly reduced the enhancing effects of CP 93129 (0.01 ćg/side) on the amphetamine-induced hyperactivity. These findings indicate that 5-HT1B receptors located in the VTA do not play a major role in the hyperlocomotion elicited by amphetamine, whereas their activation may modulate the behavioral response to the psychostimulant.

1033/2523

Tytuł oryginału: Cocaine-induced hyperactivity is more influenced by adenosine receptor agonists than amphetamine-induced hyperactivity.
Autorzy: Poleszak Ewa, Malec Danuta
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.359-366, il., bibliogr. 43 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The influence of adenosine receptor agonists and antagonists on cocaine and amphetamine-induced hyperactivity was examined in mice. All adenosine receptor agonists significantly decreased the locomotor activity in mice, and the effects were dose-dependent. It seems that adenosine A1 and A2 receptors might be involved in this reaction. Moreover, all adenosine receptor agonists: 2-p-(2-carboxyethyl)phenethylamino-5'-N-ethylcarboxamidoadenosine (CGS 21680), A2A receptor agonist, N6-cyclopentyladenosine (CPA), A1 receptor agonist, and 5'-N-ethylcarboxamidoadenosine (NECA), A2/A1 receptor agonist significantly and dose-dependently decreased cocaine-induced locomotor activity. CPA reduced cocaine action at the doses which, given alone, did not influence motility, while CGS 21680 and NECA decreased the action of cocaine at the doses which, given alone, decreased locomotor activity in animals. These results suggest the involvement of both adenosine receptors in the action of cocaine although agonists of A1 receptors seem to have stronger influence on it. The selective blockade of A2 adenosine receptor by DMPX (3,7-dimethyl-1-propargylxanthine) significantly enhanced cocaine-induced locomotor activity of animals. Caffeine had similar action but the effect was not significant. CPT (8-cyclopentyltheophylline) - A1 receptor antagonist, did not show any influence in this test. Similarly, all adenosine receptor agonists decreased amphetamine-induced hyperactivity, but at the higher doses than those which were active in cocaine-induced hyperactivity. The selective blockade of A2....

1034/2523

Tytuł oryginału: Effect of cocaine and amphetamine on biosynthesis of proenkephalin and prodynorphin in some regions of the rat limbic system.
Autorzy: Turchan Jadwiga, Maj Marcin, Przewłocka Barbara, Przewłocki Ryszard
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.367-372, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: A vast body of evidence points to the role of the limbic system in the mechanism of drug dependence. Opioid peptides localized in the limbic system may play a role in central effects of substances of abuse. The goal of the present study was to investigate the influence of acutely and chronically administered drugs of abuse, cocaine and amphetamine on biosynthesis of prodynorphin and proenkephalin in the rat amygdala, the structure involved in the mechanism of drug addiction. Acute injection of cocaine (20 mg/kg ip every hour for 3 h) or amphetamine (2.5 mg/kg) did not changed or decreased the level of proenkephalin mRNA in the central nucleus of the amygdala. In contrast, the level of prodynorphin mRNA was significantly increased in this structure after cocaine. Repeated cocaine administration (20 mg/kg ip every hour for 3h, for 5 days) had no effect on the proenkephalin and prodynorphin mRNA in the central nucleus of the amygdala. Chronic amphetamine (2.5 mg/kg twice daily for 5 days) administration decreased proenkephalin and increased prodynorphin mRNA level in the central nucleus of the amygdala (at 24 and 48 h). Moreover, significant increase in prodynorphin mRNA level was observed in the hippocampal dentate gyrus after acute (cocaine) and chronic (cocaine, amphetamine) administration of the psychostimulants. The observed adaptive changes in the activity of two opioid systems in two structures of the limbic system, central nucleus of amygdala and hippocampus, may contribute to the neurochemical mechanism of drug addiction after psychostimulants. These studies also....

1035/2523

Tytuł oryginału: Repeated treatment with selective serotonin reuptake inhibitors but not anxiolytics prevents the stress-induced deficit of fighting behavior.
Autorzy: Ossowska Grażyna, Żebrowska-Łupina Iwona, Danilczuk Zofia, Klenk-Majewska Bożena
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.373-380, il., tab., bibliogr. 46 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Several animal models of "depression" have been examined. One of them is chronic unpredictable stress (CUS)-induced deficit of fighting behavior in rats. In the present study, we compared the effects of two antidepressants (fluoxetine or fluvoxamine) and three anxiolytics (buspirone, lorazepam or oxazepam) on the electric footshock-induced fighting behavior in the pairs of male Wistar rats exposed to CUS procedure (16-day application of various unpredictable stressors). It was found that, in chronically stressed rats, the number of fighting attacks was significantly reduced (by about 70 p.c.). Prolonged (for 14 days) treatment of rats with fluoxetine or fluvoxamine (both at the dose of 10 mg/kg/day) counteracted the deficit of aggression induced by the chronic stress,. On the contrary, the anxiolytics: lorazepam (0.5 mg/kg/day), oxazepam (5 mg/kg/day) or buspirone (0.2 mg/kg/day) administered for 14 days, did not modify the deficit of fighting induced by CUS procedure. It must be underlined that prolonged treatment with all used drugs did not change the intensity of fighting in normal (unstressed) rats. In conclusion, prolonged tratment with antidepressant drugs prevents the CUS-induced deficit of fighting behavior, whereas no beneficial effect of anxiolytic agents was found.

1036/2523

Tytuł oryginału: Effect of repeated treatment with mirtazapine on the central dopaminergic D2/D3 receptors.
Autorzy: Rogóż Zofia, Wróbel Andrzej, Dlaboga Daniel, Dziedzicka-Wasylewska Marta
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.381-389, tab., bibliogr. 42 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Mirtazapine (MIR) is an antidepressant drug which enhances noradrenergic and serotonergic 5-HT1A neurotransmission via antagonistic action at central ŕ2-adrenergic autoreceptors and heteroreceptors. We reported earlier that tricyclic antidepressants administered repeatedly induced adaptive changes in the central dopaminergic D2/D3 receptors. Therefore, we designed our present study to determine whether repeated MIR treatment could evoke similar effect. The experiments were carried out on male Wistar rats. MIR was administered at a dose of 10 mg/kg once or repeatedly (twice daily for 14 days). The obtained results showed that MIR administered repeatedly potentiated the hyperlocomotion induced by D-amphetamine but not by quinpirole or 7-OH-DPAT. Biochemical study showed that MIR administered repeatedly decreased the binding of [3H]quinpirole (a D2/D3 receptor agonist) in the shell part of the nucleus accumbens septi and in the islands of Calleja but did not change the binding in the nucleus caudatus (medial or lateral). On the other hand, both acute and repeated drug treatment did not change the [3H]7-OH-DPAT (a D3 receptor agonist) binding sites in the islands of Calleja as well as in the shell part of nucleus accumbens septi. In addition, MIR did not alter the level of mRNA encoding dopamine D2 receptors, not only after repeated but also after acute treatment. The above results indicate that repeated MIR administration did not induce any adaptive change (behavioral and biochemical changes) in the dopaminergic D2/D3 system.

1037/2523

Tytuł oryginału: Pharmacological analysis of the hypothermic effects of NAN-190 and its analogs, postsynaptic 5-HT1A receptor antagonists, in mice.
Autorzy: Wesołowska Anna, Borycz Jolanta, Paluchowska Maria H., Chojnacka-Wójcik Ewa
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.391-399, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: In this study, we examined the role of 5-hydroxytryptamine (5-HT)1A and ŕ1-adrenergic receptors in the hypothermia induced by 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]-piperazine (NAN-190) and its analogs, 1-(2-methoxyphenyl)-4-[(4-succinimido)butyl]piperazine (MM77) and trans-1(2-methoxyphenyl)-4-[4-(2-phthalimido)cyclohexyl]piperazine (MP245), which - like NAN-190 - showed a high affinity for 5-HT1A and ŕ1-adrenoceptors. Administration of NAN-190 (a partial agonist of presynaptic and an antagonist of postsynaptic 5-HT1A receptors and ŕ1-adrenoceptors), MM77 and MP245 (antagonists of postsynaptic 5-HT1A receptors), as well as 8-OH-DPAT (a 5-HT1A agonist) and prazosin (an ŕ1-adrenoceptor antagonist) induced a dose-dependent hypothermia in mice. The silent antagonist of 5-HT1A receptors, WAY 100635, which abolished the hypothermic effect of 8-OH-DPAT, inhibited the hypothermia induced by NAN-190 administered at a dose of 1 mg/kg (but not 2 mg/kg) and by MP245 (0.5 and 1 mg/kg), but failed to change the MM77 (1 and 4 mg/kg)-induced decrease in body temperature in mice. The ŕ1-adrenoceptor agonist St587, which reduced the hypothermic effect of prazosin, inhibited the decrease in body temperature evoked by NAN-190 at the higher dose and by MP245at both the doses used, but did not affect the MM77-induced hypothermia in mice. The obtained results suggest that the hypothermia in mice induced by NAN-190 and its constrained analog MP245 is connected with stimulation of 5-HT1A receptors and with blockade of ŕ1-adrenoceptors, participation of these receptors not being equivalent, though. The origin of the hypothermia evoked by MM77 is still unknown.

1038/2523

Tytuł oryginału: Using reverse transcription and a competitive polymerase chain reaction for quantification of ŕ1B-adrenoceptor mRNA.
Autorzy: Kreiner Grzegorz, Sanak Marek, Zelek-Molik Agnieszka, Nalepa Irena
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.401-405, il., bibliogr. 10 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Molecular cloning studies have revealed the existence of three subtypes of ŕ1-adrenergic receptor (ŕ1-AR), namely ŕ1A, ŕ1B and ŕ1D. They are encoded by separate genes and have distinct pharmacological profiles. In rats' brain, the expression of mRNA for subtypes of an ŕ1-AR is partially structure-dependent. Our previous studies employing Northerm blot analysis of mRNA have shown that in the hippocapmus, where ŕ1A predominates, the ŕ1B receptor (ŕ1B-AR) was almost undetectable. The goal of the present study was to estabilish the method of reverse transcription and competitive polymerase chain reaction (RT-cPCR) to quantify a steady state level of ŕ1B-AR mRNA in the hippocampus, prefrontal cortex and thalamus, and to compare the ŕ1B-AR' pattern of expression with that revealed by Northern blot analysis. Our results have shown that ŕ1B-AR is similarly represented in the thalamus and prefrontal cortex. In the hippocampus, ten times lower expression of ŕ1B mRNA has been demonstrated with RT-cPCR, which was below a detection limit of Northern blot hybridization technique.

1039/2523

Tytuł oryginału: Perazine as a potent inhibitor of human CYP1A2 but not CYP3A4.
Autorzy: Wójcikowski Jacek, Pichard-Garcia Lydiane, Maurel Patrick, Daniel Władysława A.
Źródło: Pol. J. Pharmacol. 2002: 54 (4) s.407-410, il., bibliogr. 10 poz.
Sygnatura GBL: 313,156

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The effects of perazine on the activities of CYP1A2 and CYP3A4 in a primary culture of human hepatocytes of one patient were studied in vitro. The CYPs activities were assessed by measuring the rate of acetanilide 4-hydroxylation (CYP1A2) and cyclosporine A oxidation (CYP3A4) after treatment with TCDD (a CYP1A subfamily inducer) or rifampicin (mainly a CYP3A4 inducer). The amounts of the metabolities formed in hepatocytes were assayed in the extracellular medium using the HPLC method. TCDD and rifampicin induced the formation of 4-hydroxyacetanilide and cyclosporine A metabolities (monohydroxycyclosporine A, dihydroxycyclosporine A, N-desmethylcyclosporine A), respectively. The formation of 4-hydroxyacetanilide was strongly inhibited by three different concentrations of perazine (10, 25 and 50 ćM) reaching 8,3 and 2 p.c. of the control value, respectively. In the case of CYP3A4 activity, no such an effect of perazine was observed. Perazine showed only a week inhibition of the activity of cyclosporine A oxidase (to 96-86 p.c. of the control value). The obtained results suggest a strong inhibitory effect of perazine on human CYP1A2 activity with predicted Ki value similar to those of the known for CYP1A2 inhibitors, such as furafylline and fluvoxamine.

1040/2523

Tytuł oryginału: Beware the wolves are about.
Autorzy: McLellan I.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.42-44 - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1041/2523

Tytuł oryginału: Operating room air pollution - clinical experience from Germany.
Autorzy: Taeger K., Harth M.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.44-46, tab., bibliogr. 3 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

1042/2523

Tytuł oryginału: Anaesthesia and risk.
Autorzy: Aitkenhead A. R.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.49-53, tab., bibliogr. 17 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1043/2523

Tytuł oryginału: Some anesthetic aspects in vascular neurosurgery.
Autorzy: Lewelt W.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.53-55, bibliogr. 8 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1044/2523

Tytuł oryginału: Head trauma, cerebral vascular damage and secondary brain ischemia.
Autorzy: DeWitt D. S., Prough D. S.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.55-61, tab., bibliogr. 96 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1045/2523

Tytuł oryginału: The open lung management in anesthesia and intensive care.
Autorzy: Lachmann B., Haitsma J. J.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.61-64, il., bibliogr. 20 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1046/2523

Tytuł oryginału: Atelectasis during anaesthesia and methods to prevent it.
Autorzy: Hedenstierna G.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.67-69, il., bibliogr. 18 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1047/2523

Tytuł oryginału: Apneic ventilation, experimental and clinical research.
Autorzy: Babinski M. F.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.69-71 - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1048/2523

Tytuł oryginału: Non obstetric emergencies in pregnant women.
Autorzy: May A. E.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.93-94, bibliogr.7 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

1049/2523

Tytuł oryginału: Interventional neuroradiology and anesthesia.
Autorzy: Lewelt W.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.94-97, bibliogr. 5 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

radiologia

Typ dokumentu:

1050/2523

Tytuł oryginału: Are the problems of premedication solved in paediatric anaesthesia?
Autorzy: Holzki J.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.100-102, il., bibliogr. 10 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1051/2523

Tytuł oryginału: Low flow anaesthesia in infants and children.
Autorzy: Meakin G. H.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.102-105, bibliogr. 26 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

anestezjologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

niemowlęta

1052/2523

Tytuł oryginału: Significant back problems and regional anaesthesia.
Autorzy: May A. E.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.108-110, bibliogr. 12 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1053/2523

Tytuł oryginału: The history of modern CPR - in my time.
Autorzy: Baskett P. J. F.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.120-122 - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia po r.1945

1054/2523

Tytuł oryginału: Controversies in emergency medicine.
Autorzy: Dick W. F.
Źródło: Anestezjol. Intens. Ter. 2002: 34 supl. 1: XIV Międzynarodowy Zjazd Polskiego Towarzystwa Anestezjologii i Intensywnej Terapii : wykłady i streszczenia s.124-128, il., tab., bibliogr. 28 poz. - 14 Międzynarodowy Zjazd Polskiegio Towarzystwa Anestezjologii i Intensywnej Terapii Lublin 11-14.09. 2002
Sygnatura GBL: 305,376

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1055/2523

Tytuł oryginału: Age-adjustment of HRV measures and its prognostic value for risk assessment in patients late after myocardial infarction.
Autorzy: Sosnowski Maciej, MacFarlane Peter W., Czyż Zbigniew, Skrzypek-Wańha Janina, Boczkowska-Gaik Ewa, Tendera Michał
Źródło: Int. J. Cardiol. 2002: 86 (2/3) s.249-258, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aims of our study were: (1) to establish the normal limit of the heart rate variability (HRV) indices in a healthy population and in its fourage-related subgroups, including a new HRV index, HRV fraction; and (2) to analyse the frequency and predictive value of abnormally low HRV in a population of post-infarction patients in respect to the cut-points chosen (raw or age-adjusted). Methods: Normal population of 296 healthy subjects (81 f, 215 m, aged 47 ń 10 years) and post-infarction population of 298 patients ( 3 months after acute MI, 65 f, 233 m, aged 56 ń 10) were examined. The normal population was divided into 4 subsets based on age at entry: 35, 35-44, 45-54 and 54 years. Based on a 24 h ECG the standard HRV analysis was performed to obtain the following indices: mean RR interval, SDNN and SDANN. A new index of HRV, HRV fraction (HRVF, p.c.) was calculated based on a numercial processing of the RR intervals return map. All patients were followed for 24 months. The endpoints of the study were death (of any reason) and cardiac death. Results: Means and normal limits for SDNN, SDANN and HRVF were: 147 ń 36 ms [95 p.c. CI 89-220], 136 ń 36 ms [79-212] and 53 ń 9 p.c. [35-68]. The HRV values below the lower normal limit (LNL) were observed in 20-25 p.c. of post-MI patients. During a 2 year follow-up there were 36 deaths (total mortality 12.1 p.c.), while cardiac mortality was 9.1 p.c. (27 cases). The prognostic value of the analysed indices was similar (sens 53-61 p.c., spec 79-84 p.c., PPV 2-26 p.c., NPV 93-94 p.c.)...

1056/2523

Tytuł oryginału: Unexpected eosinophilic mycoarditis in a young woman with rapidly progressive dilated cardiomyopathy.
Autorzy: Bilińska Zofia T., Bilińska Maria, Grzybowski Jacek, Przyłuski Jakub, Michalak Ewa, Walczak Ewa, Wagner Teresa, Rużyłło Witold
Źródło: Int. J. Cardiol. 2002: 86 (2/3) s.295-297, ml., bibliogr. 5 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We present the case of 23-year-old woman with good living conditions, one year history of ventricular arrhythmia and 6 months history of decreased exercise tolerance, who was found to have dilated cardiomyopathy after aborted sudden death. Endomyocardial biopsy did not show specific findings. Within 3 months she develooped profound bradycardia requiring pacemaker implantation and refractory heart failure, treated with heart transplantation. Intense eosinophilic myocarditis was found in the explanted heart. Retrospective analysis of the patient's blood count revealed mild eosinophilia (eosinophil count: 0.86 x 109/1) on one examination only. Following heart transplantation the patient had persistent eisinophilia (eosinophil count: 0.62 x 109/1). Although there was no proven parasitic infestation, based on positive family history of Enterobius vermicularis infestation she was treated with broad-spectrum antiparasitic agent: albendazole and her eosinophil count returned to normal values. This case shows that active eosinophilic myocarditis may present clinically as progressive dilated cardiomyopathy with severe involvement of conduction system. Massive myocardial tissue eosinophilia occurred in the setting of mild and transient blood eosinophilia. Favourable outcome following antiparasitic treatment suggests a potential parasitic infestation as a cause of the disease.

1057/2523

Tytuł oryginału: Effect of angiotensin II on ANP-dependent guanylyl cyclase activity in cultured mouse and rat podocytes.
Autorzy: Golos Magdalena, Lewko Barbara, Bryl Ewa, Witkowski Jacek M., Dubaniewicz Anna, Olszewska Agnieszka, Latawiec Elżbieta, Angielski Stefan, Stepiński Jan
Źródło: Kidney Blood Press. Res. 2002: 25 (5) s.296-302, il., tab., bibliogr. 37 poz., sum.
Sygnatura GBL: 313,314

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The presence of a well-developed contractile apparatus is the feature determining mojor roles of podocytes in the renal glomeruli. Receptors for a variety of a variety of vasoactive hormones are expressed in these cells; however, most of the signaling pathways are still unknown and remain to be elucidated. Angiotensin II (Ang II) and atrial natriuretic peptide (ANP), due to their opposite action, are the major modulators of glomerular filtration. In podocytes, Ang II induces rise in intracellular calcium concentration, whereas ANP stimulates generation of cGMP. The present study was designed to check whether ANP-stimulated cGMP synthesis in podocytes might be affected by Ang II. Cultured rat (RP) and mouse (MP) podocytes were stimulated with ANP, in the absence or presence of Ang II and cyclic GMP was determined by RIA method. Co-incubation of podocytes with ANP and Ang II caused significant (p 0.01) suppression of ANP-dependent cGMP generation. The effect was prevented by saralasin, an inhibitor of angiotensin receptors. Phorbol-12-myristate-13-acetate (PMA) mimicked, whereas chelerythrine reversed inhibitory effect of Ang II. In conclusion, angiotensin II counteracts ANP-stimulated cGMP synthesis in cultured podocytes. It seems likely that the protein kinase C pathway is involved in this effect.

1058/2523

Tytuł oryginału: The nephrological issues raised during the clinical sessions in the Old Order (Jewish) Hospital of Warsaw.
Autorzy: Rybus-Potępa Elżbieta, Marczewski Krzysztof
Źródło: Nephrol. Dial. Transplant. 2002: 17 (12) s.2084-2086, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 312,872

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XIX wieku

1059/2523

Tytuł oryginału: Discrepancies in creatinine clearance in centenarians when calculated by two different mathematical formulas.
Autorzy: Wieczorowska-Tobis Katarzyna, Mossakowska Małgorzata, Niemir Zofia, Bręborowicz Andrzej, Oreopoulos Dimitrios G.
Źródło: Nephrol. Dial. Transplant. 2002: 17 (12) s.2274-2275, bibliogr. 4 poz.
Sygnatura GBL: 312,872

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1060/2523

Tytuł oryginału: Cell cycle in sporadic melanoma.
Autorzy: Czajkowski Rafał, Drewa Tomasz, Woźniak Alina, Krzyżyńska-Malinowska Ewa
Źródło: Int. J. Dermatol. 2002: 41 (9) s.550-556, il., bibliogr. 53 poz.
Sygnatura GBL: 305,645

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Sporadic melanoma is a neoplasm whose etiology has been fully investigated. Contemporary achievements in molecular biology have made it possible to localize the genes whose damage can contribute to the initiation of neoplastic transformation of malanocytes and lead to a progression of the disease. The majority of these genes are responsible for the correct progression of phase G1 of the cell cycle. Phase G1 of the cell cycle is subject to control by many protooncogenes and antioncogenes, wihich constitute the pRb or p53 pathway, damage to which can lead to the development of malignant melanoma. The present paper discusses disorders inthe control of phase G1 of the cell cycle in sporadic melanoma.

1061/2523

Tytuł oryginału: Oxidative stress and neutrophil activation - the two keystones of ischemia/reperfusion injury.
Autorzy: Kamiński Karol A., Bonda Tomasz A., Korecki Janusz, Musiał Włodzimierz
Źródło: Int. J. Cardiol. 2002: 86 (1) s.41-59, il., bibliogr. 212 poz.
Sygnatura GBL: 305,693

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The widespread introduction of fibrinolytics and recently also PTCA in the treatment of myocardial infarction has changed the picture of modern cardiology. But this therapy also raises new problems and challenges. One of them is the occurrence of ectensive tissue injury caused by reperfusion. Reinstitution of oxegen to the ischemia tissues initiates various processes leading to generation of reactive oxygen species (ROSs). Acting on the plasma membrane ROS damage its organization and release various proinflammatory agents. Different proteins, including receptors, ionic channels, transporters or components of transduction pathways are substrates of oxidation by ROSs. Theis changed structure results in altered functioning and disruption of vital cellular processes. Another key factor of reperfusion injury is activation and infiltration of infarcted are by polymorphonuclear leukocytes (PMNs). Multiple studies identified stages of PMN activation and substances being involved in it. Main interest lies in cellular adhesion molecules, particularly selectins and á2 integrins, as their atagonists were repeatedly found to diminish neutrophil activation and infarct size. Nevertheless new publications strike at the foundations of the established order and confront the realtion between neutrophil infiltration and infarct size. PMNs are linked by close ties to other cells involved in inflammatory response. Seemingly also in cardiac ischemia-reperfusion injury, the activity of neutrophils is modulated by lymphocytes and macrophages. The article describes mutual interactions between different factors involved in the reperfusion injury that may enable preparing new treatments, hopefully as effective and successful as reperfusion therapy.

1062/2523

Tytuł oryginału: Effect of acute exercise on the content of free sphinganine and sphingosine in different skeletal muscle types of the rat.
Autorzy: Dobrzyń A., Górski J.
Źródło: Horm. Metab. Res. 2002: 34 (9) s.523-529, il., tab., bibliogr. 31 poz.
Sygnatura GBL: 312,226

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie polskie: It has previously been shown that prolonged exercise of moderate intensity reduces the content of ceramide in each type of skeletal muscle. This was accompanied by a reduction in the activity of neutral, Mg++-dependent sphingomyelinase (the major enzyme responsible for ceramide formation from sphingomyelin) in the soleus and red gastrocnemius, but not in the white gastrocnemius (A. Dobrzyń and J. Górski, Am. J. Physiol.: Endocrinol. Metab. 282: E277 - E285, 2002). No other data on regulation of ceramide metabolism in contracting muscles are available. The aim of the present study was to examine the content of sphinganine (a key precursor of ceramide on the de novo synthesis route) and the content of sphingosine (the main product of ceramide catabolism) in different skeletal muscle types after two kinds of acute exercise. The experiments were carried out on 30 male Wistar rats, 250-280 g of body weight. The rats were divided equally into three groups: 1 - control, 2 - run until exhaustion (1200 m/h, + 10ř incline), 3 - a group in which the sciatic nerve was stimulated 10 min with tetanic pulses (60 pulses/min). Samples were taken of the soleus and of the red and white section of the gastrocnemius. These muscles are composed mostly of the slow-twitch oxidative, fast-twitchoxidative-glycolytic and fast-twitch glycolytic fibers, respectively. Lipids were extracted with chloroform/methanol. Sphinganine and sphingosine were quantified by high-performance liquid chromatography. Atrest, the content of sphinganine in ...

1063/2523

Tytuł oryginału: Expression of very late antigen-4 and lymphocyte function-associated antigen-1 on peripheral blood lymphocytes from patients with graves disease.
Autorzy: Bossowski Artur, Urban Mirosława, Stasiak-Barmuta Anna, Turowski Dariusz
Źródło: Pediatr. Res. 2002: 52 (4) s.533-537, il., bibliogr. 31 poz.
Sygnatura GBL: 300,504

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the syudy was to estimate the expression of á1 integrin CD49d (very antigen-4), á2 integrin CD11a (lymphocyte function-associated antigen 1 alpha), l-selectin (CD62L), and intercellular adhesion molecule-1 [ICAM-1 (CD54)] on peripheral blood mononuclear cells in patients with Graves disease (GD0 (n = 45, mean age 15.9 ń 5.9 y), in patients with nontoxic nodular goiter (NTNG) (n = 25, mean age 15.2 ń 5.7 y), and in sex- and age-matched healthy control subjects (n = 25, mean age 15.9 ń 2.4 y). The expression of the lymphocyte adhesion molecules was analyzed by three-color flow cytometry. decreased percentages of CD49d+ and CD11a+ lymphocytes were observed in untreated Graves' patients in comparison to healthy controls (p 0.007, p 0.036) and non-toxic nodular goiter patients (p 0.006, p 0.019). The analysis of CD62L+ and CD54+ antigen expression on peripheral blood lymphocytes revealed increased percentages of L-selectin- and ICAM-1-positive cells in patients with GD (p 0.005 for CD62L, p 0.008 for ICAM-1) before methimazole therapy, compared with healthy controls. These abnormalities were absent in children with NTNG. In patients with unterated GD, a positive correlation was found between serum levels of free thyroxine and the percentage of CD54+ lymphocytes (r = 0.47, p 0.036). Statistically significant negative correlations existed between free tyroxine concentration in blood serum and the percentage of CD11a+ lymphocytes (r = -0.39,p 0.033). No such correlation was detected between the percentage of lymphocyte markers and serum levels of antithyroid antibodies...

1064/2523

Tytuł oryginału: Changes in the volume of temporal lobe structures related to Alzheimer's type dementia.
Autorzy: Moryś Janusz, Bobek-Billewicz Barbara, Dziewiątkowski Jerzy, Bidzan Leszek, Ussorowska Dorota, Narkiewicz Olgierd
Źródło: Folia Neuropathol. 2002: 40 (2) s.47-56, il., tab., bibliogr. 59 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We compared the volumes of the superior temporal gyrus, basolateral temporal area, parahippocampal gyrus, hippocampal head, amygdaloid body and the inferior horn of the lateral ventricle in 29 patients with dementia of Alzheimer's type (DAT) and in 14 cognitively normal controls. Measurements were performed on coronal MR images perpendicular to the long axis of the hippocampus then the raw data were normalised for intracranial volume. The volumes of all studied structures were significantly msaller in patients with DAT than in age-matched control group. The differences in the volume between DAT and age-matched control group were the largest for the amygdaloid body and the basolateral temporal area. The discriminant analysis including the volume of both the basolateral area and the left amygdaloid body allows for correct classification of 100 p.c. DAT patients and 93 p.c. controls. We conclude that the estimation of the volume of the amygdaloid body and basolateral temporal area seems to be the most important factor for DAT diagnosis.

1065/2523

Tytuł oryginału: An attempt at evaluating borderline conditions of Parkinson's disease and its preclinical stage on the basis of clincial and morphological correlation.
Autorzy: Krygowska-Wajs Anna, Adamek Dariusz, Szczudlik Andrzej, Kałuża Józef
Źródło: Folia Neuropathol. 2002: 40 (2) s.57-66, il., tab., bibliogr. 19 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the investigations was to find to what extent neurodegenerative changes develop in the brains of patients with no clinical symptoms of dementia, parkinsonism and other neurodegenerative diseases. It has been found that neurodegenerative pathology, as evaluated using immunohistochemical methods with monoclonal antibodies (Mab) against ubiquitin, tau protein, ŕ-synuclein, and á-amyloid, occurs more frequently than the presence of Lewy bodies. The degenerative changes involved the neurones of cerebral and cerebellar cortex, basal ganglia and medulla oblongata, where neurofibrillary tangles were found. Mab positive materials have been found in the cytoplasm of the cell body and the cell processes (axons) of the neurones and glial cells. Senile plaques, á-amyloid positive, were frequently noted.

1066/2523

Tytuł oryginału: Abnormal nerve conduction velocity as a marker of immaturity in childhood muscle spinal atrophy.
Autorzy: Krajewska Grażyna, Hausmanowa-Petrusewicz Irena
Źródło: Folia Neuropathol. 2002: 40 (2) s.67-74, il., tab., bibliogr. 28 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Motor conduction velocity (CV) was tested in 117 infants and children with proximal spinal muscular atrophy (SMA), aged 1-53 months, and in 99 age-adjusted healthy controls. The children were classified into SMA forms 1 and 2 according to SMA International Consortium Criteria. In all children CV was tested in four nerves and the following parameters were taken into account: distal latency, conduction velocity, amplitude of muscle response. The electromyography of muscles revealed neurogenic changes. In all cases of severe form 1 motor CV was markedly slowed, with significantly prolonged distal latency adn reduced amplitude of M response. In surviving children CV, although increasing with age, was always below the values of normal age-adjusted children. The slowing was particularly pronounced in the femoral nerve. In SMA2 children the values of all tested parameters wre closer to the values of age-adjusted healthy children, nevertheless they were always below the edge of normal values, also the rate of maturation was much slower than in normal children. Sensory CV was normal children. Sensory CV was normal in all tested groups. The findings are suggestive of delayed nerve maturation in childhood SMA.

1067/2523

Tytuł oryginału: Suspensional reaggregates of human foetal neocortex and tegmentum as objects of neurotransplantation.
Autorzy: Bystron Irina Pavlovna, Smirnov Eugene Borisovich, Otellin Vladimir Alexandrovich, Wierzba-Bobrowicz Teresa, Dymecki Jerzy
Źródło: Folia Neuropathol. 2002: 40 (2) s.75-85, il., bibliogr. 25 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Suspensional reaggregates were obtained from human neocortical and tegmental anlagen (7 weeks of gestation), using 0.1 p.c. tripsin solution, and cultivated in Medium 199. Suspensional reaggregates, formed after 2 days in vitro, were grafted into the Wistar rat striatum. Incipient stages of histogenesis in the reaggregates and their interaction with host brain were investigated using light and electron microscopy, with antibodies against vimentin, glial fibrillary acidic protein (GFAP), proliferating cell nuclear antigen (PCNA), ferritin, as well as lectin ricinus communis agglutinin (RCA). The reaggregates showed a low level of tissue organistaion. An intermediate condition between suspension and the true tissue could be observed in them. These reaggregates had two evident features: a rather irregular cell arrangement (without parallel bundles of radial glia), and the presence of special intercellular junctions. Some cells made up fragments of neuroepithelial sheet in the form of true rosettes. The one-week-old grafts were integrated with the host brain as well as dissociated and contained host astrocytes. Degenerated cells and detritus appeared rarely. The data of this work let us conclude that the suspensional aggregate grafting combines some advantages of suspensional and solid grafting methods.

1068/2523

Tytuł oryginału: Ultrastructure of synaptic junctions in the cerebellar cortex in experimental valproate encephalopathy and after terminating chronic application of the antiepileptic.
Autorzy: Sobaniec-Łotowska Maria E.
Źródło: Folia Neuropathol. 2002: 40 (2) s.87-96, il., bibliogr. 44 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of the study was to analyse in TEM the evolution of changes in structural elements of synaptic junctions of the cerebellar cortex in rats in valproate encephalopathy induced by chronic 12-month administration of sodium valproate - VPA (once daily intragastrically, in a dose of 200 mg/kg b.w.) and after withdrawal of this antiepileptic for 1 and 3 months. After 9 and 12 months of the experiment, synaptic endings of both the symmetrical and asymmetrical synapses in the neuropil of the cerebellar cortex, especially in the molecular layer, showed signs of sever damage (mainly swelling) and even disintegration. They were mostly observed in axodendritic endings and axospinal endings on the dendritic spines of Purkinje cells, being manifested in teh presence of large vacuolar structures, electron lucent areas and swollen mitochondria within the cytoplasm. A reduced number of axonal synaptic vesicles (with more type F vesicles preserved) could be seen. One and 3 months after the end of chronic application of VPA, the synaptic junctions did not show morphological exponents of the repair processes. The alterations observed in the synaptic junctions of the cerebellar cortex may suggest disorders in neurotransmission processes, such as exhaustion and damage caused by ischaemia due to damage to the blood-brain barrier induced by VPA and/or its toxic metabolites.

1069/2523

Tytuł oryginału: Studies on the pharmacokinetics of total and free valproate in mono- and bitherapy with carbamazepine in epileptic children and adolescents.
Autorzy: Steinborn Barbara, Galas-Zgorzalewicz Bożena
Źródło: Folia Neuropathol. 2002: 40 (2) s.97-100, tab., bibliogr. 25 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: The aim of this study was to obtain a pharmacokinetic calculation for valproic acid (VPA) and its free fraction in 50 children and adolescents (4-18 years) treated for epilepsy in VPA monotherapy and bitherapy with carbamazepine (CBZ). The diurnal fluctuation of serum concentration of total and free VPA during monotherpay was observed. The additional antiepileptic medication of VPA and CBZ was connected with prominent diurnal free VPA serum fluctuations. Pharamcokinetic parameters of total and free VPA in monotherapy were significantly different. The change of free and total VPA pharamcokinetics during bitherapy with CBZ was observed, too. No changes in half-life time of VPA in mono- and bitherpay were noticed. THe variability of pharmacokinteic parameters of free VPA suggests the need for monitoring unbound VPA plasma concentrations during bitherpay with CBZ.

1070/2523

Tytuł oryginału: Application of organotypic hippocampal culture for study of selective neuronal death.
Autorzy: Sarnowska Anna
Źródło: Folia Neuropathol. 2002: 40 (2) s.101-106, il., bibliogr. 13 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: The organotypic hippocampal culture model was established according to the Stoppini method. THe transversal hippocampal slices from 7-day-old Wistar rats were cultured 14 days in vitro then subjected to acute metaboilc stress induced either by glucose deprivation or by additional treatment with a chemical respiratory chain inhibitor-cyanide in 3 mM concentration. The rate of cell death was monitored up to several hours post-treatment in living tissue by propidium iodide staining or by a trypan-blue exclusion test. No signs of selective vulnerability of any particular hippocampal region or cellular layers to applied metabolicinsults were found. Slices demonstrated total resistance even to prolonged (24 h) abscence of glucose in the culture medium, whereas the cyanide caused death which was almost immediate and unspecific to any region cells. In contrast, induction of oxidative stress by hydrogen peroxide treatment resulted in concentration and time-dependent neuronal death preferentially observed in CA1 sector of pyramidal layer. Both clinical and experimental studies confirm that the hippocampus, and particularly its CA1 sub-field, is exceptionally vunerable to ischaemic and other brain injuries. The causative role of free radical generation and the resulting oxidative stress have been widely discussed in these pathologies. Our results imply that CA1 pyramidal cells do not differe from the other hippocampal neurones in their reaction to metabolic stress. On the contrary, and from mainly unknown reasons, these CA1 neurones are extremely sensitive to the reactive oxygen-triggered damage.

1071/2523

Tytuł oryginału: Coexistence of diffuse large B-cell lymphoma within pontocerebellar angle schwannoma.
Autorzy: Kolasa Paweł, Kordek Radzisław
Źródło: Folia Neuropathol. 2002: 40 (2) s.107-110, il., bibliogr. 16 poz.
Sygnatura GBL: 304,961

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We present an unusual case of diffuse large B-cell lymphoma within pontocerebellar angle shwannoma in 62-year-old woman. The patient suffered for 5 months with V, VII and VIII nerves paresis and with cerebellar ataxia. CT scan demonstrated large hyperdensive mass in cerebellopontine angle translocating cerebellar hemisphere and cerebral trunk. The patient was subjected to surgery and the tumour was removed totally by suboccipital retromastoidal right craniectomy approach. Histopathological examination revealed schwannoma infiltrated with high grade B-cell lymphoma. The patient did well following surgery without any other lymphoma manifestations, and she died from a heart attack 20 months later. Solitary lymphoma of pontocerebellar angle coexisting with schwannoma is an unusual finding, thus our case is the first report.

1072/2523

Tytuł oryginału: Eleven years intraoperative ATG bolus. A list of successes.
Autorzy: Kaden Jrgen
Źródło: Ann. Transplant. 2002: 7 (3) s.4-10, il., tab., bibliogr. 43 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Infection therapy for organ transplantation using monoclonall antibodies has been in recent years reevaluated. Results from various centers indicate the value of such therapy, especially in sensitized patients undergoing kidney transplantation as well as in simultaneous kidney - pancreas transplant patients. Author presents the experience of eleven years of intraoperative ATG bolus administration in the Berlin - Friedrichshain Kidney Transplantation Center.

1073/2523

Tytuł oryginału: Mechanisms of ischemic preconditioning and its application in transplantation.
Autorzy: Kosieradzki Maciej
Źródło: Ann. Transplant. 2002: 7 (3) s.12-20, il., bibliogr. 96 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Ischemic preconditioning is a phenomenon of increased tissue resistance to ischemic injury evoked by brirf intermittent periods of ischemia. Since its discovery in 1986, extensive studies were undertaken to explain its mechanisms and induce protection pharmacologically. There is convincing evidence on adenosine receptors., ATP-dependent potassium channels, nitric oxide and tyrosine kinases pathways involvement in the process. Universal ability of various tissues to adopt protection from short-time ischemia and reperfusion makes clinical applications of the phenomenon, including field of transplantation medicine, highly appealing. This review summarizes known physiological pathways of ischemic preconditioning. Trials on protection against cold ischemia and other transplant-related issues of preconditioning are also presented.

1074/2523

Tytuł oryginału: Experience with ATG short course high dose induction therapy in a series of 112 enteric drained pancreatic transplants.
Autorzy: Bonatti H., Berger N, Kafka R., Tabbi M. G., Knigsrainer A., Margreiter R., Steurer W.
Źródło: Ann. Transplant. 2002: 7 (3) s.22-27, il., bibliogr. 13 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: New immunosuppressive protocols and advanced surgical technique resulted in a major improvement in the outcome of pancreatic transplantation. Patients and methods: 112 enteric drained whole pancreas transplants (PTx) performed at the Insbruck University Hospital between 3.1997 and 10.2001 were retrospectively analysed. Prophylactic immunosuppression consisted of FK506, MMF and steroids. A short course of high dose ATG induction was given additionally. Perioperative antimicrobial prophylaxis consisted of Amoxicllin/Clavulanic (32 PTx), Pipercillin/Tazobactam (68 PTx), quinolones (10 PTx) or macrolide (2 PTx). 64 patients additionally received fluconazole. Results: Actuarial patient, pancreas and kidney graft survival at one year were 96.4 p.c., 86.7 p.c. and 95.3 p.c., surgical complication rate was 28 p.c., rejection rate 40 p.c.. Eight grafts were lost due to intraabdominal infection, seven due to rejection. Median perioperative observation days (OD) were 29 (range 14-125), patients were on antibiotics for 68 p.c. of OD, and developed fever on 33 p.c. of OD. Incidence of CMV infection was 42 p.c. (bat only five patients developed CMV disease), HSV 24 p.c., intraabdominal infection 22 p.c., UTI 11 p.c., wound infection 9 p.c. and pneumonia: 5 p.c. Conclusion: ATG short course induction is well tolerated after enteric drained PTX. Infection represents a frequent and at least for IA sepsis serious complication after PTx with enteric drainage.

1075/2523

Tytuł oryginału: Freedom from rejection and stable kidney function are excellent criteria for steroid withdrawal in tacrolimus-treated kidney transplant recipients.
Autorzy: Włodarczyk Z., Wałaszewski J., Perner F., Vitko S., Ostrowski M., Bachleda P., Kokot F[ranciszek], Klinger M., Szenohradsky P., Studenik P., Navratil P., Asztalos L., Rutkowski B., Nagy K. Karmar, Hickey D.
Źródło: Ann. Transplant. 2002: 7 (3) s.28-31, il., tab., bibliogr. 5 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Objectives: This prospective, randomized, multicentre study investigated the efficacy and safety of two tacrolimus-based regimens and their potential to withdraw steroids. Methods: In total 489 patients were randomised to receive either tacrolimus and MMF (n=243) or tacrolimus and azathioprine (n=246) concomitantly with steroids in both treatment groups. The initial oral dose of tacrolimus was 0.2 mg/kg/day, MMF dose was 1 g/day, azathioprine was administered at 1-2 mg/day. Steroids were tapered from 20 mg/day to 5 mg/day. From month 3 onwards, steroids were withdrawn in patients who were free from steroid-resistant rejection and who had serum creatinine concentrations 160 ćmol/L. Study duration was 6 months. Results: Patient survival at month 6 was 98.3 p.c. (Tac/MMF/S) and 98.4 p.c. (Tac/AzaS), graft survival at 6 month was 95.0 p.c. (Tac/MMF/S) and 93.5 p.c. (Tac/Aza/S). The 6-month incidences of biopdy-proven acute rejection were 18.9 p.c. (Tac/MMF/S compared with 28.8 p.c. (Tac/Aza/S), p=0.038. The 6-month incidence of steroid-resistant acute rejection were 2.1 p.c. (Tac/MMF/S) and 4.9 p.c. (Tac/Aza/S), p=ns. At the end of month 3, steroid withdrawal was performed in 60.5 p.c. (Tac/MMF/S) and 48.8 p.c. (Tac/Aza/S) of patients, p 0.01. During months 4-6, 2.7 p.c. of patients in the Tac/MMF group had a biopsy-confirmed acute rejection compared with 0.8 p.c. of patients in the Tac/Aza group. In patients who continued to receive steroids, the incidences of biopsy-proven acute rejection during months 4-6 were 3.5 p.c. (Tac/MMF/S) and 132,8 ćmol/L (Tac/Aza/S). Conclusion: Both tacrolimus regimens are efficacious and safe. The combination of Tacrolimus and MMF achieved a lower rejection rate and permitted a higher proportion of steroid-free patients. Ther overall incidence of acute rejection was low and kidney function was good.

1076/2523

Tytuł oryginału: DNA from rejecting donor organs can be detected in recipient lymphoid and non-lymphoid tissues.
Autorzy: Interewicz B., Olszewski W. L., Maksymowicz M., Stanisławska J., Szyper E.
Źródło: Ann. Transplant. 2002: 7 (3) s.32-35, il., bibliogr. 11 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Rejecting tissue and organ grafts shed cellular debris from damaged cells. Cellular debris contains fragments of nuclei with their genetic material. The question arises what is the fate of donor. DNA in recipient fluids and tissues. Is it enzymatically disintegrated and becomes a waste product or it is utilized by recipient cells. We have shown, using sex-mismatched transplants and the Sry-gene fragment PCR detection method, that at the time of rejection recipient tissue contain donor DNA. The concentration of donor DNA did not paralled the concentration of live donor passenger cells. There were differences in donor DNA concentration depending on whether heart, skin or BMC were transplanted. Moreover, there was more donor DNA in recipient tissues than in control syngeneic transplants. Interestingly, a relatively, a high donor DNA concentration was detected in syngeneic recipients reflecting the extent of non-immune pre-transplantation ischemic damage of the graft.

1077/2523

Tytuł oryginału: Serum cytokine concentration after liver lobe harvesting for transplantation.
Autorzy: Słotwiński R., Olszewski W. L., Paluszkiewicz R., Zieniewicz K., Hevelke P., Zaleska M., Krawczyk M., Krasnodębski I. W.
Źródło: Ann. Transplant. 2002: 7 (3) s.36-39, il., bibliogr. 11 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: To investigate cytokine concentration after liver lobe harvesting for transplantation in order to prove that it is a relatively safe surgical procedure with limited tissue injury. Material and methods: Study was performed in 17 healthy liver donors and 6 patients with benign liver tumors. Serum levels of IL-6, IL-Ira and sTNFRI were measured before surgery and on the days 1, 3 and 7 thereafter, by immunoassay (ELISA). Results: There a significant increase in serum concentrations of IL-6 on day 1-7 (from 2.4 pg/ml to 315.1ń526, 47.3ń48 and 15.3ń15pg/ml; p=0.0002, p=0.0006, p=0.003), for IL-Ira on day 1-3 (from 472.5ń436 pg/ml to 2072.6ń3511 and 715.5ń268 pg/ml; p=0.001, p=0.004), and for sTNFRI on day 1-3 (from 1075.7ń338.0 pg/ml to 1601.4ń317.0 and 1528.9ń402.0 pg/ml; p=0.0006, p=0.003), following liver harvesting. No significant difference was observed between pre and postoperative IL--6 serum concentration, whereas IL-Ira and sTNFRI were elevated only on day 3 after liver resection (p=0.02, p=0.04. A significantly higher level of sTNFRI was seen in patients after liver resection, as compared to liver donors on day 1 (p=0.01), 3 (p=0.03) and 7 (p=0.0006) after surgery, whereas on day 3 (p=0.03) and 7 (p=0.0) when IL-Ira was measured. Conclusion: The short period of elevated concentrations of IL6, sTNFRI and ILIra after harvesting of lobes for transplantation and relatively low serum level of these cytokines indicate that this type of procedure does not evoke any major postoperative SIRS-type reaction as seen not infrequently after resection of liver for primary or secondary liver pathology.

1078/2523

Tytuł oryginału: Induction of long term peripheral microchimerism in non-human primates in a model of xenogeneic peripheral tolerance induction.
Autorzy: Simon A. R., Schrder C., Martin U., Tessmann R., Templin C., Laaf G., Wiebe K., Steinhoff G., Lapin B., Chikobava M., Haverich A.
Źródło: Ann. Transplant. 2002: 7 (3) s.40-45, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

małpy

Świnie

Streszczenie angielskie: Xenotransplantation represents a promising solution to the ever increasing shortage of donor organs in allotransplantation. However, due to different and stronger models of rejection, successful xenotransplantation will require different organ-protective regimes from those used in allogenic transplantation today. Since one can simply increase the dosage of the drugs used, immunomodulation or tolerance induction of the recipient would be the most desirable approach. Transfusion of donor leukocytes has been shown to downregulate recipient responses or even induce peripheral tolerance in small animal models. Since the infusion of donor cells represents a relatively simple approach, as one can purify and compose the inoculum exactly before infusion, we studied whether this approach can be successfully employed in a preclinical swine to nonhuman primate model of peripheral tolerance induction/immunomodulation. In our model, baboons underwent sequential column adsorption and complement blockade. The animals received only initial immunosuppression with cyclophosphamide. No further immunosuppression was given. Subsequently all animals received 1-3x10**10 porcine splenocytes i.v. Development and maintainance of chimerism was analyzed by sequential flow cytometric and PCR analyses. Other parameters studied included effects of the preparatory induction protocol. We could show that a low level of chimerism is maintained in these animals for up to 1,5 years, despite the fact that they received no additional immunosuppression after the initial one. At no time of the experiment did any animal...

1079/2523

Tytuł oryginału: High duodeno-jejunal anastomosis as a safe method of enteric drainage in simultaneous pancreas and kidney transplantation.
Autorzy: Ziaja Jacek, Wullstein Christoph, Woeste Guido, Bechstein Wolf Otto
Źródło: Ann. Transplant. 2002: 7 (3) s.46-49, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: On the key issues in successful pancreas transplantation is uncomplicated drainage of pancreas exocrine secretion. Objective: The aim of the study is to present results of side-to-side high duodeno-jejunal anastomasis as routine method of enteric drainage in simultaneous pancreas kidney transplantation (SPK). Methods: 30 diabetic patients underwent SPK at the Department of Surgery, Ruhr University Bochum in 2001. The pancreas was drained using a portion of duodenal segment anastomosed to the first loop of jejunum about 20-40 cm distal to the Treitz ligament. Results: Early relaparotomy was required in 20 p.c. patients. The mean time of the first relaparotomy was 5,5 (range 1-110 days after transplantation. In 10 p.c. cases graft pancreatectomy was necessary. Perioperative mortality was 3,3 p.c. Currently 83,3 p.c. patients are insulin-free and 86,6 p.c. patients are free of dialysis. Conclusions: These data suggest, that side-to-side high duodeno- jejunal anastomosis is a safe method of drainage of pancreas exocrine secretion in SPK.

1080/2523

Tytuł oryginału: Application of recombinant activated factor VII for treatment of impaired haemostasis during liver transplantation in recipients with Wilson's disease - a report of two cases.
Autorzy: Jureczko L[idia], Trzebicki J., Zawadzki A., Pacholczyk M., Łągiewska B., Kołacz M., Szyszko G., Mayzner-Zawadzka E.
Źródło: Ann. Transplant. 2002: 7 (3) s.52-54, tab., bibliogr. 6 poz.
Sygnatura GBL: 313,259

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Recombinant activated factor VII (rFVIIa, NovoSeven, Novo Nordisk A/S, Denmark) is a treatment used to prevent and arrest intra- and postoperative bleeding in patients with haemophilia A or B complicated by circulating anticoagulants (inhibitors of FVII and FIX) and in patients without haemophilia who spontaneously develop inhibitors of FVIII, i.e. in acquired haemophilia. Patients who qualify for liver transplantation due to liver dysfunction may have varying degrees of coagulation impairment and thus carry an elevated risk of massive bleeding and have worse prognosis. The authors administered recombinant activated factor VII to two patients with coagulation abnormalities in the couse of Wilsons's disease during liver transplantation.

1081/2523

Tytuł oryginału: Distribution of actin in etoposide-induced human leukemia cell line K-562 using fluorescence and immunoelectron microscopy technique.
Autorzy: Grzanka Alina, Grzanka Dariusz
Źródło: Pol. J. Pathol. 2002: 53 (2) s.43-50, il., bibliogr. 26 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Localization of actin was studied in erythroleukemic cell line K-562 after treatment with etoposide for 72 hours in a range of concentration 0.02 - 200 ćM/L. Actin was visualised by fluorescence microscopy and streptavidingold method. These findings indicate that changes in actin after treatment with etoposide were dose-dependent. Significant changes in the cellular distribution of F-actin in K-562 cells were obtained after treatment with 20 and 200ćM/L etoposide. In comparison with control cells, the number of the cells decreased and cells were larger especially at 200ćM/L. F-actin was diffusely distributed throught the cell at 20ćM/L. Treatment of cells with 200ćM/L etoposide showed F-actin diffusely distributed throughout the cell with local actin assemblies and also at the cell periphery. Immunogold labelling of actin was observed in cells treated with all doses of etoposide and control cells. Labelling was found in the nucleus and also in the cytoplasm. At the ultrastructural level, cells treated with 200ćM/L etoposide showed protrusions at the surface, in which increase of actin was often observed. Etoposide causes changes in actin distribution of K-562 cells, and the changes in expression of actin were not only restricted to cell with features of apoptosis.

1082/2523

Tytuł oryginału: Pattern of renal á1(ŕ1 - ŕ6) integrins distribution in IgA nephropathy and Henoch-Schoenlein nephritis.
Autorzy: Wągrowska-Danilewicz Małgorzata, Danilewicz Marian
Źródło: Pol. J. Pathol. 2002: 53 (2) s.51-57, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Immunoperoxidase staining was carried out using monoclonal antibodies against integrins ŕ1á1, ŕ2á1, ŕ3á1, ŕ4á1 and ŕ6á1 on renal biopsy specimens from patients with IgA nephropathy (IgN, n = 15) and Henoch-Schoenlein nephritis (HSN, n = 10). The basic pattern of glomerular and tubulointerstitial (ŕ1 - á6)á1 integrin distribution was similar in both studied groups, however increase in mesangial ŕ1á1 integrin immunoexpression in biopsies from HSN patients as compared to biopsies from IgAN patients was observed. There were no statistical differences in the intensity of tubulointerstitial (ŕ1 - ŕ6)á1 integrins immunolabelling in renal tissue between IgAN and HSN patients. The similar pattern of distribution of á1 integrins in renal tissue in IgAN and HSN patients may support the hypothesis of common pathogenesis of IgAN and HSN. Upregulation of ŕ1á1 integrin on mesangial regions in biopsy specimens in patients with HSN may be connected to the much more florid glomerular changes in renal tissue in this type of glomerulonephritis than in IgAN.

1083/2523

Tytuł oryginału: Effect of melatonin and all-trans retinoic acid on the proliferation and induction of the apoptotic pathway in the culture of human breast cancer cell line MCF-7.
Autorzy: Czeczuga-Semeniuk Ewa, Wołczyński Sławomir, Anchim Tomasz, Dzięcioł Janusz, Dąbrowska Milena, Pietruczuk Mirosława
Źródło: Pol. J. Pathol. 2002: 53 (2) s.59-65, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Melatonin in the in vitro conditions inhibits cell growth and proliferation of estrogen sensitive (ER+) cell line MCF-7 in culture. In the present study, during a 48-hour incubation melatonin at a concentration of 10-**5M inhibited [3H]thymidine incorporation into cancer cells at the level of 69.52 p.c. ń 10.99 p.c. Melatonin had no inhibitory effect on the physiological stimulatory action of estradiol. Tamoxifen added to the medium modulated the melatonin action only when the latter was added 24 hours after tamoxifen (46.45 p.c. ń 4.40, p 0.05). Tretinoin added to the culture caused a statistically significant reduction in [3H]thymidine incorporation into the cancer cells, compared to he melatonin and tretinoin groups, when treatment with retinoid was synergistic (39.05 p.c. ń 5.44, p 0.05) or sequential (tretinoin and after 24h melatonin) (39.96 p.c. ń 1.55, p 0.05). This was confirmed by immunocytochemical investigations, which showed a statistically significant reduction in the percentage of PCNA- and Ki67-positive cells. Apart from the inhibitory effect on MCF-7 cell proliferation retinoids induce the apoptotic pathway in a dose-dependent manner. Melatonin added to the culture enhances this effect, which may indicate the potential for the use of both substances in the treatment of breast cancer in women.

1084/2523

Tytuł oryginału: Estrogen and progesterone receptors in neoplastic and non-neoplastic thyroid lesions.
Autorzy: Lewy-Trenda Iwona
Źródło: Pol. J. Pathol. 2002: 53 (2) s.67-72, tab., bibliogr. 42 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The pathogenesis of thyroid cancer is complex. Iodium deficiency, genetic factors, sex, older age, irradiation in childhood, thyroid growth stimulating antibodies and epithelial growth factor possibly affect its development. Recent reports, also epidemiological, have shown that differentiated thyroid cancer (papillary, follicular, oxyphillic) is dependent on sex hormones, especially estrogens. This has prompted research into the presence of estrogens and progesterone, as well as androgen receptors (ER, PR and AR) in normal and neoplastic thyreocytes and estradiol content in thyroid tissue. The results of these investigations imply that thyroid cancer are estrogen-dependent. There is, however, no agreement in reports about correlation between tumor malignancy and ER, PR and AR expression. In this study 72 thyroid glands have been examined using immunohistochemical assays with ERs and PRs antibodies. Positive staining was demonstrated mainly in differentiated thyroid cancer nuclei (17 ERs positive and 2 PRs positive). There was no expression of antibodies in non-neoplastic cells or in adjacent tissues. The nuclei of 3 oxyphillic and 4 follicular adenomas were also ERs positive. On the other hand nuclei of 5 fokkicular adenomas were PRs positive. These results are in agreement with most available data. It seems, however, that the material so far examined by the investigators is too small. Furthermore, because of using various methods the results cannot be compared. Further studies are necessary to reveal if there is any true influence of sex hormones on the development of thyroid lesions and if the detection of sex hormone receptors may help in choosing adequate therapy.

1085/2523

Tytuł oryginału: Indocyanine green angiography in the diagnosis of small choroidal tumours.
Autorzy: Kubicka-Trząska Agnieszka, Starzycka Maria, Romanowska-Dixon Bożena
Źródło: Ophthalmologica 2002: 216 (5) s.316-319, il., tab., bibliogr. 10 poz.
Sygnatura GBL: 300,913

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

ludzie

Streszczenie angielskie: To evaluate indocyanine green (ICG) angiography in the diagnosis of small choroidal tumours. Methods: We studied 40 patients with small chorodial tumours, 22 males and 18 females. The tumour thickness on ultrasonography ranged from 2.2 to 4.3 mm. Results: Nineteen pigmented tumours showed early and late diffuse hypofluorescence, consistent with naevi. Eight non-pigmented tumours showed normal choroidal vessels in calibre, configuraiton, and branching, typical of non-pigmented naevi. The early frame of ICG angiography showed in 13 cases pathological, intrinsic vascularization, suggestive of melanomas. Conclusion: This preliminary study suggests that ICG angiography may be helpful in differentiating choroidal naevi from small melanomas.

1086/2523

Tytuł oryginału: Treatment of neuropathic urinary and faecal incontinence.
Autorzy: Skobejko-Włodarksa L.
Źródło: Eur. J. Pediatr. Surg. 2002: 12 (5) s.318-321, il., tab., bibliogr. 10 poz., Zsfg, rs., res.
Sygnatura GBL: 312,942

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 13-18 r.ż.

Streszczenie angielskie: The author presents 14 children after meningomyelocoele repair with faecal and urinary incontinence, aged from 6 to 17 years who have undergone the MACE procedure. MACE with synchromous Mitrofanoff continent stoma creation was performed in one child with severe steniosis of the urethra. MACE with simultaneous bladder augmentation was used in 10 patients. 5 of them underwent colocystoplasty, 3 had ileocystoplasty and 2 ureterocystoplasty. Of these 10 children, three have additionally undergone the Mitrofanoff procedure. patients' follow-up ranged from six months to two years. Three children had trouble with the MACE stoma because of its stenosis. One of them required revision of the stoma. All patients became clean and dry, and this operation improved not only their quality of life, but also their independence.

1087/2523

Tytuł oryginału: Transabdominal ultrasound for visualizing gastric submucosal tumors diagnosed by endosonography: can surveillance be simplified?
Autorzy: Polkowski M., Palucki J., Butruk E.
Źródło: Endoscopy 2002: 34 (12) s.979-983, il., tab., bibliogr. 23 poz.
Sygnatura GBL: 305,439

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background an study aims: Management options for gastric submucosal tumors (SMTs) include tumor removal or surveilance. If the latter is chosen, it requires repeated measurements of the tumor diameter. Although this can be achieved using endoscopy or endosonography (EUS), a less invasive and cheaper method would be welcome. THe aim of this prospective study was to assess the proportion of gastric SMTs that can be visualized using transabdominal ultrasound of the water-filled stomach. Patients and methods: Fifty-one consecutive patients with endosonographically diagnosed gastric SMTs underwent transabdominal ultrasound examinations of the water-filled stomach performed immediately after EUS; both procedures were carried out by the same investigator. Transabdominal ultrasound was considered positive only if: firstly, the tumor was visualized unequivocally; secondly, its dimensions could be measured; and thirdly, photographic documentation could be recorded. In each case, a positive result had to be confirmed by an independent in vestigator, who reviewed the photographic documentation. Results: The median size of SMTs on EUS was 25 mm (range 4-55 mm). Twelve tumors were located in the antrum, 25 in the gastric body, and 14 in the gastric fundus or cardia. Transabdominal ultrasound demonstrated the tumor in 35 of 51 patients (69 p.c.). For tumors ó 30 mm, the visualization rate was 61 p.c. (22 of 36). The location of the tumor and its EUS features did not significantly affect the sensitivity of transbdominal ultrasound. Conclusions:...

1088/2523

Tytuł oryginału: Primenenie videotorakoskopii v khirurgicheskom lechenii miastenii.
Tytuł angielski: Application of videothoracoscopy in surgical treatment of myasthenia.
Autorzy: Peljukhovskij S. V.
Źródło: Klin. Khir. 2002 (8) s.23-24, bibliogr. 11 poz., sum.
Sygnatura GBL: 303,753

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

praca opublikowana za granicą

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: In 39 patients with various forms of myasthenia operation thymectomy was performed, including those 9 (20.5 p.c.), to whom it was done using method of videothoracoscopy. There were no postoperative complications. Good cosmetic effect was noted, the duration of hospital treatment of the patietns had reduced more than twice, causing economic effect.

1089/2523

Tytuł oryginału: B cell-dependent T cell responses: IgM antibodies are required to elicit contact sensitivity.
Autorzy: Tsuji Ryohei F., Szczepanik Marian, Kawikova Ivana, Paliwal Vipin, Campos Regis A., Itakura Atsuko, Akahira-Azuma Moe, Baumgarth Nicole, Herzenberg Leonore A., Askenase Philip W.
Źródło: J. Exp. Med. 2002: 196 (10) s.1277-1290, il., bibliogr. 59 poz.
Sygnatura GBL: 310,177

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Contact sensitivity (CS) is a classic example of in vivo T cell immunity in which skin sensitization with reactive hapten leads to immunized T cells, which are then recruited locally to mediate antigen-specific inflammation after subsequent skin challenge. We have previously shown that T cell recruitment in CS is triggered by local activation of complement, which generates C5a that triggers C5a receptors most likely on mast cells. Here, we show that B-1 cell-derived antihapten IgM antibodies generated within 1 day (d) immunization combine with local challenge antigen to activate complement to recruit the T cells. These findins overturn three widely accepted immune response paradigms by showing that (a) specific IgM antibodies are required to initiate CS, which is a classical model of T cell immunity thought exlusively due to T cells, (b) CS priming induces production of specific IgM antibodies within 1 d, although primary antibody responses typically begin by day 4, and (c) B-1 cells produce the 1-d IgM response to CS priming, although these cells generally are thought to be nonresponsive to antigenic stimulation. Coupled with previous evidence, our findings indicate that the elicitation of CS is initiated by rapidly formed IgM antibodies. The IgM and challenge antigen likely form local complexes that activite complement, generating C5a, leading to local vascular activation to recruit the antigen-primed effector T cells that mediate the CS response.

1090/2523

Tytuł oryginału: Amifostine differentially modulates DNA damage evoked by idarubicin in normal and leukemic cells.
Autorzy: Błasiak Janusz, Gloc Ewa, Młynarski Wojciech, Drzewoski Józef, Skórski Tomasz
Źródło: Leuk. Res. 2002: 26 (12) s.1093-1096, il., bibliogr. 8 poz.
Sygnatura GBL: 306,151

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Human lymphocytes, p53 protein-deficient acute promyelocytic leukemia cell line HL-60, murine pro-B lymphoid cell line BaF3 and its TEL/ABL-transformed clone cells were exposed to idarubicin with and without pre-treatment with amifostine. Idarubicin at 0.5 - 5ćM evoked DNA damage measured by the Comet assay. Amifostine at 14 mM decreased DNA-damaging effect of idarubicin in human lymphocytes and BaF3 cells, but increased the effect in TEL/ABL-transformed cells. Amifostine had no influence on the action of idarubicin in HL-60 cells. Our results suggest that the reaction of the cell to DNA damage may contribute to its diverse response to amifostine combined with anticancer drugs and that p53 and fusion tyrosine kkinases may be involved in this diveristy.

1091/2523

Tytuł oryginału: European Society of Cardiology Congress 31 August - 4 September 2001, Berlin - Germany: abstracts.
Źródło: Eur. Heart J. 2002: 23 supl. s.I-XVI, 1-791, il., tab. - Kongres Europejskiego Towarzystwa Kardiologicznego Berlin 31.08-04.09. 2002
Sygnatura GBL: 306,213

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Temat korporatywny:

Europejskie Towarzystwo Kardiologiczne

1092/2523

Tytuł oryginału: Karol Marcinkowski University of Medical Science, Poznań Faculty of Medicine Division of Dentistry : course credit transfer system : information package 2001/2002
Opracowanie edytorskie: Przystanowicz Aleksander (ed.), Rębała Leszek (ed.), Shaw Geoffrey (ed.).
Źródło: - Poznań, Dział Wydaw. Uczelnianych AM 2002, 73, [1] s., [3] k. tabl. : il., tab., 31 cm.
Sygnatura GBL: 802,193

Hasła klasyfikacyjne GBL:

stomatologia

Typ dokumentu:

informator

tytuł obcojęzyczny

Temat korporatywny:

Akademia Medyczna w Poznaniu

1093/2523

Tytuł oryginału: Ultrastructural studies of experimental scrapie and Creutzfeldt-Jakob disease in hamsters. [P.] 1: Alterations of myelinated axons.
Autorzy: Liberski Paweł P., Bratosiewicz-Wąsik Jolanta, Gajdusek D. Carleton, Brown Paul
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.121-129, il., bibliogr. s. 127-129
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

Streszczenie angielskie: Classical and ultrastructural neuropathology of prion diseases are generally well described. Here we report that alternations of myelinated fibres in hamsters infected either with polioencephalopathic strains of scrapie or panencephalopathic strains of CJD (Echigo-1) are virtually identical and differ only quantitatively. In contrast, mice infected with the panencephalopathic Fujisaki strain of CJD exhibited much more elaborate changes of myelinated fibres.

1094/2523

Tytuł oryginału: Ultrastructural studies of experimental scrapie and Creutzfeldt-Jakob disease in hamsters. [P.] 2: Astrocytic and macrophage reaction towards axonal destruction.
Autorzy: Liberski Paweł P., Bratosiewicz-Wąsik Jolanta, Gajdusek D. Carleton, Brown Paul
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.131-139, il., bibliogr. s. 137-139
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

Świnki morskie

Streszczenie angielskie: We report hare the microglial (macrophage) and astrocytic reaction in several models of transmissible spongiform encephalopathies (TSEs) or prion diseases. With the low power electron microscopy it was readily apparent that myelinated vacuoles were surrounded by cells and their processes. The latter belonged either to hyperplastic reactive astrocytes or to macrophages. Typically, reactive astrocytes exhibited cytoplasm filled with innumerable glial filaments and, occasionally, other organelles (like cilia) and abundant tortuous intercellular junctions of adhesive plaque junction type. Desmosome-like junctions connecting astrocytic elements were also seen. As described earlier, astrocytic process were occasionally interdigitated with oligodendroglial cells and their processes. Two types of macrophages were readily described. The majority of them exhibited electron-dense cytoplasm and numerous "empty" vacuoles (digestive chambers) containing cellular debris. Occasional vacuoles were surrounded by a thin collar reminiscent of "lyre-like inclusions" of the second type of macrophages. Several mylinated fibres were clearly engulfed by the cytoplasm of a macrophage containing unusual annulate lamellae.

1095/2523

Tytuł oryginału: How do neurons degenerate in prion diseases or transmissible spongiform encephalopathies (TSEs): neuronal autophagy revisited.
Autorzy: Liberski Paweł P., Gajdusek D. Carleton, Brown Paul
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.141-147, il., bibliogr. s. 145-147
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

chomiki

Streszczenie angielskie: As in other neurodegenerative diseases such as Alzheimer's disease, neurons in prion diseases or transmisible spongiform encephalopathies (TSEs) die via programmed cell death of which the apoptotic process is relatively well characterized. A subcellular alteration linked to apoptosis is the formation of autophagic vacuoles, which we and others demonstrated in CJD- and scrapie-affected rodent brains. Autophagy may co-exist with apoptosis or may precede it and the process may be induced by apoptosis stimuli. Here, we extend these observations using different model of scrapie and CJD. Both scrapie models (the 265K and 22C-H) demonstrated autophagic vacuoles with the same frequency; hence, they will be described together. While the following changes had been observed simultaneously in different areas of the same sample, this description is organised as if it followed a sequence of events. First, a part of the neuronal cytoplasm was sequestrated by concentric arrays of membrane; that part of the cytoplasm closed by membranes appeared relatively normal but its density often appeared increased. Next, electron density of the central dramatically increased. Then, membranes proliferated within the cytoplasm in a labyrinth-like manner and an area sequestrated by these membranes enlarged and became more complex structure consisting of vacuoles, electron-dense area and areas of normally-looking cytoplasm connected with convoluted membranes. Finally, a large area of the cytoplasm was transformed into a collection of autophagic vascuoles of different sizes. Virtually identical alternations, albeit with much lower frequency, were seen in terminally ill CJD-affected hansters.

1096/2523

Tytuł oryginału: Codon 219 in Creutzfeld-Jakob disease in Poland.
Autorzy: Bratosiewicz-Wąsik Jolanta, Wąsik Tomasz J., Liberski Paweł P.
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.149-151, il., bibliogr. s. 151
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

komunikat

Wskaźnik treści:

in vitro

Streszczenie angielskie: Prion diseases are a group of etiologically heterogenous diseases. In addition to familial cases linked to mutations of PRNP open reading frame they include also cases of unknown etiology. One of the susceptibility factors to sporadic as well as iatrogenic prion diseases are PRNP polymorphisms. In the present study, we analyzed sequences of the PRNP gene codon 219 of 16 Polish CJD cases and we found heterozygous GAG to GAT changes on the sense strand and only wild type sequences on an antisense strand. The RFLP technique was used to verify this divergence and only wild type sequences were revealed.

1097/2523

Tytuł oryginału: Molecular biology of prions.
Autorzy: Weissmann Charles, Enari Masato, Klhn Peter Christian, Rossi Daniela, Flechsig Eckhard
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.153-166, il., tab., bibliogr. [111] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The "protein only" hypothesis holds that the infectious agent causing transmissible spongiform encephalopathies is a conformational isomer of PrP, a host protein predominantly expressed in brain and is strongly supported by many lines of evidence. Prion diseases are so far unique among conformational diseases in they are transmissible, not only experimentally but also by natural routes, mainly by ingestion. The pathway of prions to the brain has been elucidated in outline. A striking feature of prions is their extraordinary resistence to conventional sterilisation procedures, and their capacity to bind to surfaces of metal and plastic without losing infectivity. This property, first observed in a clinical setting, is now being investigated in experimental settings, both in animals in cell culture.

1098/2523

Tytuł oryginału: Variant Creutzfeldt-Jakob disease.
Autorzy: Will Robert George
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.167-173, il., tab., bibliogr. [18] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Variant Crutzfeldt-Jakob disease is caused by the transmission of bovine spongiform encephalopathy to humans. The clinical and investigative features of variant CJD are relatively distinct from sporadic CDJ. The number of cases of vCJD are increasing with time in the UK, but the total future number of cases of vCJD is uncertain.

1099/2523

Tytuł oryginału: Neuropathology of variant Creutzfeldt-Jakob disease.
Autorzy: Ironside James W., Head Mark W., McCardle Linda, Knight Richard
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.175-182, il., tab., bibliogr. s. 181-182
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The clinical, neuropathological genetic and biochemical features of variant Creutzfeldt-Jakob disease (vCJD) are compared to the 926 other cases of suspected CJD referred to the National CJD Surveillance Unit laboratory from 1999 - 2001. Histological studies of the central nervous system, lymphoid tissues and other organs were accompanied by immunocytochemistry for prion protein (PrP); Western blot analysis of PrP**RES was performed on frozen brain tissue. The pathology of vCJD showed relatively uniform morphological and immunocytochemical characteristics, with PrP accumulation in lymphoid tissues, but not in other non-neural tissues. PrP**RES accumulation in vCJD showed a uniform glycotype pattern distinct from sporadic CJD. All cases of vCJD were methionine homozygotes at codon 129 of the PrP gene. In view of the spread of bovine spongiform encephalopathy in Europe and Japan, continuing surveillance is required for all forms of CJD, with histological and biochemical analysis of suspected cases to allow an accurate laboratory diagnosis.

1100/2523

Tytuł oryginału: Bovine spongiform encephalopathy. Update.
Autorzy: Bradley Ray
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.183-195, il., tab., bibliogr. [24] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

weterynaria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

koty

Streszczenie angielskie: Bovine spongiform encephalopathy (BSE) is a zoonosis being the origin of variant Creutzfeldt-Jakob disease and an important cattle in its own right. Countries have been slow to learn the importance of protecting, not only their cattle populations, but also their human populations. Since 2000, several additional European countries have reported BSE in native-born stock and this has led to a concern about the BSE status of countries that have imported cattle and cattle products from infected countries. Extensive feed and offal bans and application of newly-developed, 'Rapid' tests for prion protein in central nervous tissue of targeted, high-risk animals and slaughter cattle over 30 months old now provides the tools whereby the public are fully protected and BSE can be eradicated.

1101/2523

Tytuł oryginału: Prion diseases: a dual view of the prion hypothesis as seen from a distance.
Autorzy: Liberski Paweł P., Jaskólski Mariusz
Źródło: Acta Neurobiol. Exp. 2002: 62 (3) s.197-226, il., tab., bibliogr. [269] poz.
Sygnatura GBL: 302,090

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: We review the historical background and principles of the prion theory in its current shape. We showed that most of data may be still interpreted dually according to the protein only hypothesis and according to the theory in which additional component is necessary to comprise the infectivity. The enormous impact of structural biological studies is also stressed.

1102/2523

Tytuł oryginału: Plasma tissue inhibitor of metalloproteinases-1 and transforming growth factor á1 - possible non-invasive biomarkers of hepatic fibrosis in patients with chronic B and C hepatitis.
Autorzy: Flisiak Robert, Maxwell Paul, Prokopowicz Danuta, Timms Peter M., Panasiuk Anatol
Źródło: Hepatogastroenterology 2002: 49 (47) s.1369-1372, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 304,355

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

badanie porównawcze

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background/Aims: The role of transforming growth factor-á1 (TGF-á1) in liver fibrosis is in part related to impairment of extracellular matrix breakdown by stimulation of tissue inhibitor of metalloproteinases-1 (TIMP-1) gene. The aim of he study was to evaluate association between TGF-á1 and TIMP-1 in relation to liver injury in chronic viral hepatitis N and C. Methodology: Association between plasma TGF-á1 and TIMP-1 was evaluated in 28 consecutive patients undergoing kiver biopsy for chronic viral hepatitis B and C (CH-B, CH-C) and these tests were correlated with hepatic fibrosis, inflammation and liver function tests. Moreover carboxyterminal cross-linked telopeptide of type 1 procollagen (ICTP) and carboxyterminal propeptide of type 1 collagen (PICP) were also measured for assessment of extracellular matrix breakdown or synthesis, respectively. Results: Chronic viral hepatitis B and C resulted in a significant increase in plasma TIMP-1 levels but not TGF-á1. Among biochemical markers of liver injury, significant correlation with TGF-á1 and TIMP-1 was demonstrated in respect to aminotransferase activities in both groups. TIMP-1 showed significant correlation with ICTP levels in both CH-B (r = 0.59) and CH-C (r = 0.62), whereas TGF-á1 was correlated with ICTP only in CH-C patients (r = 0.75). PICP did not demonstrate any corelation with either TGF-á1 or TIMP-1. Hepatic fibrosis, but not inflammation, correlated significantly with TGF-á1 (CH-B: r = 0.73; CH-C: r = 0.79) and TIMP-1 (CH-B: r = 0.66; CH-C r = 0.71) in both groups and there was a significant...

1103/2523

Tytuł oryginału: Complexed prostate-specific antigen, complexed prostate-specific antigen of total and transition zone, complexed/total prostate-specific antigen ratio, free-to-total prostate-specific antigen ratio, density of total and transition zone prostate-specific antigen: results of the prospective multicenter european trial.
Autorzy: Djavan Bob, Remzi Mesut, Zlotta Alexandre R., Ravery Vincent, Hammerer Peter, Reissigl Andreas, Dobroński Piotr, Kaisary Amir, Marberger Michael
Źródło: Urology 2002: 60 (4a) suppl. s.4-9, tab., bibliogr. 30 poz.
Sygnatura GBL: 312,634

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: This prospective, multicenter European Prostate Cancer Detection study evaluated the value and performance of the molecular forms of prostate-specific antigen (PSA) and their derivatives in combinantion with prostate gland and transition zone volumes in early detection of prostate cancer in patients with PSA levels between 4 and 10 ng/mL. Of 750 men enrolled at 7 different European urology centers into the study between November 2001 and March 2002, 340 (45.3 p.c.) had a total PSA (tPSA) between 4 and 10 ng/mL (age range, 46 to 87 years). In all patients, the ratio of complexed PSA (cPSA) to tPSA (c/tPSA), cPSA density (cPSAD), cPSAD of the transition zone, PSA, free (fPSA), ratio of fPSA to tPSA (f/tPSA), tPSA density (PSAD), and PSAD of the transition zone were measured and collected 5 to 10 minutes before the sextant biopsy with 2 additional transition zone cores. Measurements of tPSA and fPSA were done with the AxSYM test, whereas cPSA was measured with the ACS 180 cPSA assay. All patients had a transrectal ultrasound-guided sextant prostate biopsy, and 2 additional transition zone bipsies and total and transition zone volumes were measured at the time of biopsy. Histopathologic findings revealed benign in 237 patients and prostate cancer in 103 patients (69.7 p.c. and 30.3 p.c., respectively). Statistically significant differences included larger total volumes, larger transition zone volumes, and f/tPSA in patients with benign disease (P = 0.0009, P 0.0001, P 0.0001, respectively). At 90 p.c. and 95 p.c. sensitivity, specificity of cPSA was significantly...

1104/2523

Tytuł oryginału: DNA microarrays - a methodological breakthrough in genetics.
Autorzy: Kamiński Stanisław
Źródło: J. Appl. Genet. 2002: 43 (2) s.123-130, il., bibliogr. [18] poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

1105/2523

Tytuł oryginału: Journal of Applied Genetics
Tytuł angielski: Novel linkage mapping approach using DNA pooling in human and animal genetics. [P.] 1: Detection of complex disease loci.
Autorzy: Pareek Chandra S., Pareek Ravi S., Walawski Krzysztof
Źródło: J. Appl. Genet. 2002: 43 (2) s.175-192, il., tab., bibliogr. [48] poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: DNA pooling is a potential methodology for genetic loci with small effect contributing rapid preliminary screening of the genome for the allelic association with the most common class of polymorphic short tandem repeart markers. The methodology assumes as a common founder for the linked disease locus of interest and searches for a region of a chromosome sheared between affected individuals. The general theory of DNA pooling basically relies on the observed differences in the alletic distribution between pools from affected and unaffected individuals, including a reduction in the number of alleles in the affected pool, which indicate the sharing of a chromosomal region. The power od statistic for associated linkage mapping can be determined using two recently developed strategies, firstly, by measuring the differences of allelic image patterns produced by two DNA pools of extreme character and secondly, by measuring total allele content differences by comparing between two pools containing large numbers of DNA samples. These strategies have effectively been utilized to identify the shared chromosomal regions for linkage studies and to investigate the candidate disease locci for fine structure gene mapping using allelic association. This paper outlines the utilization of DNA pooling as a patential tool to locate the complex disease loci, statistical methods for accurate estimates of allelic frequencies from DNA pools, its advantagess, drawbacks and significance in associate linkage mapping using pooled DNA samples.

1106/2523

Tytuł oryginału: Clinical features, treatment and genetic background of Treacher Collins syndrome.
Autorzy: Marszałek Bożena, Wójcicki Piotr, Kobus Kazimierz, Trzeciak Wiesław H.
Źródło: J. Appl. Genet. 2002: 43 (2) s.223-233, il., tab., bibliogr. [24] poz.
Sygnatura GBL: 305,055

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Treacher Collins syndrome (TCS) is an autosomal dominant disorder of craniofacial development. The major features of the disease include midface hypoplasia, micrognathia, microtia, conductive hearing loss and cleft palate. Current procedures of surgical treatment of TCS are discussed and novel findings concerning the genetic backgroung of TCS are described. The TCS locus has been mapped to chromosome 5q31.3-32. The TCOFI gene contains 26 exons and encodes a 1411 amino acid protein named treacle. In the TCOFI gene 51 mutations have been identified. Most of these mutations are insertions or deletions, which result in an introduction of a premature terminantion codon into the reading frame. Mutational spectra support the hypothesis that TCS results from haploinsufficiency of treacle.

1107/2523

Tytuł oryginału: Fluctuactions of gastrin. Motilin and total bile acid levels in porcine blood before and after feeding.
Tytuł polski: Zmienność poziomu gastryny, motyliny i kwasów żółciowych w surowicy świń przed i po karmieniu.
Autorzy: Romański Krzysztof W., Salomon Ewa, Borodulin-Nadzieja Ludmiła, Janocha Anna
Źródło: Folia Med. Crac. 2002: 43 (1/2) s.5-12, tab., bibliogr. 42 poz., streszcz.
Sygnatura GBL: 304,697

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

Świnie

Streszczenie polskie: U czterech młodych, zdrowych świń wykonywano radioimmunologiczne badania poziomu gastryny w surowicy, motyliny w osoczu oraz poziomu sumy kwasów żółciowych w surowicy krwi w ciągu czterech godzin okresu międzytrawiennego oraz w okresie do jednej godziny po zastoswaniu standardowej procedury karmienia. U zwierząt głodzonych 24 godziny, stężenia gastryny, motyliny i kwasów żółciowych wykazywały oscylacje i czas trwania okresów oscylacji był podobny do czasu trwania cyklu motorycznego w okresie międzytrawiennym. Jednakże tylko piki poziomu motyliny były czasowo skorelowane z pikami poziomu kwasów żółciowych. Karmienie nie znosiło oscylacji poziomu motyliny i kwasów żółciowych oraz istotnie obniżało poziom kwasów żółciowych w surowicy. Zatem współzależność pomiędzy poziomami gastryny, motyliny i kwasów żółćiowych różnią się częściowo u świń od tych u człowieka i psa; ponadto pozostaje szereg niejasności odnośnie regulacyjnej roli obu badanych hormonów, zwłaszcza w okresie poposiłkowym.

Streszczenie angielskie: In four yount healthy pigs, the radioimmunological determinations of serum gastrin, plasma motilin and total serum bile acids were performed during four hours of the interdigestive state and up to one hour after standard feeding procedure. In 24-hr fasted animals, gastrin, motilin and bile acid concentrations fluctuated and duration of oscillation periods was similar to the length of the interdigestive motor cycle. However only motilin peaks were temporally correlated with bile acid peaks. Feeding did not abolish motilin and bile acid fluctuations and significantly decreased serum bile acid level. It is concluded that the relationships among gastrin, motilin and bile acids are in part different in pigs from those in man and dog and still some controversy exists as to the regulatory role of both hormones mainly in the postprandial state.

1108/2523

Tytuł oryginału: Interdigestive contractions of the gallbladder in rabbits: the real time ultrasonographical studies.
Tytuł polski: Skurcze międzytrawienne pęcherzyka żółciowego u królików. Badanie USG.
Autorzy: Romański Krzysztof W., Woźniak-Stolarska Barbara
Źródło: Folia Med. Crac. 2002: 43 (1/2) s.13-20, tab., bibliogr. 32 poz., streszcz.
Sygnatura GBL: 304,697

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie polskie: Brak doniesień dotyczących ultrasonograficznego badania kurczliwości pęcherzyka żółciowego u królika. Zatem celem obecnych badań była ocena dynamiki skurczów pęcherzyka żółciowego u 7 królików. Zwierzęta karmiono 24 godziny przed doświadczeniami, które trwały 3-5 godzin. Badania ultrasonograficzne przeprowadzano co 10 minut używając aparatu typu USG 220 SLC. Sektorową sondę 3,5 MHz przykładano w rejonie prawego podżebrza. Uzyskiwano maksymalne podłużne obrazy pęcherzyka żółciowego i szacowano objętość pęcherzyka żółciowego stosując 3 metody. Najwyższe wartości otrzymano dzięki użyciu emtody przybliżającej kształt pęcherzyka żółciowego do elipsoidy. Stosując metodę przybliżania kształtu pęcherzyka żółciowego do beczki otrzymano wyniki pośrednie, a najniższe wartości otrzymano metodą sumy cylindrów. Wartość średnia wszystkich objętości pęcherzyka żółciowego uzyskanych ostatnią metodą wynosiła 1,34 ń 0,10 ml. Wartości szczytowe i międzyszczytowe wynosiły odpowiednio 2,23 ń 0,33 i 0,99 ń 0,18 ml. Średnia odległość pomiędzy pikami wynosiła 69,6 ń 7,4 min pozostając w przybliżeniu zgodną z opisaną w piśmiennictwie długością cykli MMC u królika. Wyniki sugerują, że model ultrasonografii pęcherzyka żółciowego królika może być ogólnie użyteczny w badaniach dynamiki pęcherzyka żółciowego.

Streszczenie angielskie: There are no reports concerning the ultrasonographical examination of gallbladder contractility in rabbits. Thus, the aim of this study was to assess the dynamics of gallbladder contractions in 7 rabbits. Animals were fasted 24 h before the experiments lasting 3-5 h. Ultrasonographical examinations were performed every 10 min using USG 220 SLC machine. The sectorial ultrasound 3.5 MHz was placed in the right subcostal region. The maximal longitudinal gallbladder scans were obtained and the gallbladder volume was estimated using 3 methods. The highest values were obtained with the method approximating the gallbladder shape to ellipsoid. The second method approximating gallbladder shape to the barrel provided intermediate results and the lowest results were obtained with the sum-of-cylinders method. The overall mean of interdigestive gallbladder volume using the latter method was 1.34 ń 0.10 ml. Peak and interpeak values were 2.23 ń 0.33 and 0.99 ń 0.18 ml, respectively, p 0.01. The mean peak distance was 69.6 ń 7.4 min approximately remaining in concert with the reported MMC intervals in rabbits. It is concluded that the rabbit model for gallbladder ultrasonography may be useful for the study of gallbladder dynamics.

1109/2523

Tytuł oryginału: The usefulness of low-pressure pneumoperitoneum in laparoscopic surgery.
Tytuł polski: Przydatność techniki odmy niskociśnieniowej w chirurgii laparoskopowej.
Autorzy: Barczyński Marcin, Herman Roman Maria
Źródło: Folia Med. Crac. 2002: 43 (1/2) s.43-50, tab., bibliogr. 50 poz., streszcz.
Sygnatura GBL: 304,697

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Celem pracy była ocena przydatności techniki odmy niskociśnieniowej (ON) w chirurgii laparoskopowej. Grupę kolejnych 152 chorych z objawową niepowikłaną kamicą pęcherzyka żółciowego zakwalifikowanych do zabiegu cholecystektomii laparoskopowej (LC) przeanalizowano co do możliwości wykonania zabiegu w technice ON. Zastosowano następujące kryteria wyłączenia: BMI 27 oraz laparotomia w wywiadzie. Spośród 152 chorych jedynie 82 (53,9 proc.) chorych spełniających kryteria włączenia do bdania zakwalifikowano do zabiegu w technice ON (73 kobiety i 9 mężczyzn). BMI wynosił średnio 25,23 ń 1,4; średni wiek wynosił 46 ń 7,8 lat. Odmę otrzewnową wytwarzano za pomocą igły Veressa do ciśnienia 12 mmHg, a pierwszy troakar TERNAMIAN-EndoTip zakładano od kontrolą optyki. Po wkłuciu pozostałych troakarów roboczych, ciśnienie odmy otrzewnowej obniżano do 7 mmHg. W czasie zabiegu operacyjengo monitorowano chorych kapnograficnzie. Spośród 82 chorych włączonych do badania LC w technice ON wykonano z powodzeniem u 73 chorych (89 proc.). U 8 (9,75 proc.) chorych (wszyscy byli płci męskiej, a 3 miało ponad 55 lat) ciśnienie odmy otrzewnowej zwiększono do 15 mmHg z uwagi na długość pęcherzyka żółciowego przekraczającą 12 cm i niewystarczającą ekspozycję okolicy trójkąta Calota. u 1 chorego (1.22 proc.) wykonano konwersję do laparotomii z powodu trudności w identyfikacji struktur anatomicznych w obrębie trójkąta Calota. LC może być wykonana w technice ON u większości pacjentów z BMI 27, ...

Streszczenie angielskie: The aim of the study was to assess the usefulness of low-pressure pneumopertoneum (LP) in laparoscopic surgery. A consecutive group of 152 patietns qualified for laparoscopic cholecystectomy (LC) was assessed as to the possibility of performing the procedure with LP 7 mmHg. The following exclusion criteria were used: Body Mas Index (BMI) 27 and laparotomy in medical history. The subsequent stage of the trial included 82 (53.9 p.c.) patietns (73 females, 9 males) meeting the inclusion criteria. The mean BMI was 25.23 ń 1.4; mean age was 46 ń 7.8 years. All the patietns suffered from symptomatic uncomplicated gallstones confirmed in ultrasound. The pneumoperitoenum was created in a blind way with a Veress needle up to pressure of 12 mmHg and the first 10mm TERNAMIAN-EndoTip trocar was introduced under direct visual control. After the placement of other trocars, the working pressure was reduced from 12mmHg to 7mmHg. The pCO2 concentration was monitored intraoperatively by a capnometer. Among 82 patients included into the study the LPLC was successfully performed in 73 (89 p.c.) patietns. In 8 (9.75 p.c.) patietns the working pressure had to be increased to 15 mmHg due to the gallbladder exceeding 12cm in length and difficulties in its proper mobilization required to visualize the neck of the gallbladder. All these patietns were male and 3 of them were 55 years of age or more. In 1 (1.22 p.c.) patient conversion was necessary because of difficulties in identification ...

1110/2523

Tytuł oryginału: Influence of different pressures of pneumoperitoneum on the autonomic system function during laparoscopy.
Tytuł polski: Wpływ ciśnienia odmy otrzewnowej na czynność autonomicznego układu nerwowego w trakcie laparoskopii.
Autorzy: Barczyński Marcin, Herman Roman Maria
Źródło: Folia Med. Crac. 2002: 43 (1/2) s.51-58, tab., bibliogr. 16 poz., streszcz.
Sygnatura GBL: 304,697

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Wpływ ciśnienia odmy otrzewnowej na aktywność autonomicznego układu nerwowego (AUN) nie była dotychczas szczegółowo analizowana. Do prospektywnego badania włączono 20 chorych zakwalifikowanych do cholecystektomiii laparoskopowej. Zrandomizowano ich do dwóch 10-osobowych grup: odmy niskociśnieniowej (LP = 7 mmHg) i odmy standardowej (SP = 12 mmHg). Wprowadzenie do znieczulenia ogólnego uzyskiwano fentanylem i thiopenthalem, które podtrzymywano isofluranem. Wentylację kontrolowano mechanicznie (18 oddechów na minutę, końcowowydechowa prężność CO2 30-35 mmHg, ciągłe monitorowanie kapnograficzne). Ciśnienie odmy otrzewnowej utrzymywano za pomocą automatycznego insuflatora Co2. Pacjenci byli utrzymywani w pozycji leżącej w czasie całego badania. Czynność AUN oceniano za pomocą analizy zmienności rytmu serca (HRV), wykonując śródoperacyjnie 3 pomiary po 5 minut: przed wprowadzeniem do znieczulenia, po wprowadzeniu i po wytworzeniu odmy otrzewnowej. W ocenie HRV wykorzystano analizę widmową niskich (LF = 0,05 - 0,15 Hz) i wysokich (HF = 0,15 - 0,45 Hz) komponent częstotliwości za pomocą szybkiej transformacji Fourierowskiej. Przeprowadzono anlizę statystyczną uzyskanych wyników. Składowa LF uległa obniżeniu po wprowadzeniu do znieczulenia a zwiększyła się po wytworzeniu odmy w obu grupach (p 0,01). LP w mniejszym stopniu aktywowała AUN niż SP (LF: 68,12 ń 6,56 nu vs. 78,26 ń 5,43 nu, p 0,01; HF: 24 ń 3,48 vs. 15.56 ń 2,54 nu, p 0,05). Reasumując, wytworzenie odmy ...

Streszczenie angielskie: The effects of different pressure values of pneumoperitoneum on the activity of the autonomic nervous system (ANS) have not been investigated in detail. In this prospective study, 20 patietns qualified for laparoscopic cholecystectomy were randomised to either low-pressure (LP = 7 mmHg) or standard-pressure (SP = 12 mmHg) pneumoperitoneum groups, 10 patients each. The anesthesia was induced with fentanyl and thiopental and maintained with isoflurane. Ventilation was mechanically controlled (18 breaths per minute, end-tidal CO2 30-35 mmHg, instant capnometer monitoring). Intraabdominal pressure was maintained automatically by a CO2 insufflator. Patient were kept in a horizontal position. The ANS was evaluated by heart rate variability (HRV) analysis (POSTER ECG 2002 System) measured intraoperatively in three recordings of 5 minutes each: in awake patient, after induction of general anesthesia, and after creation of pneumoperitoneum. Power spectral analysis of HRV was applied, the low-frequency (LF = 0.05 - 0.15 Hz) and high frequency (HF - 0.15 - 0.45 Hz) spectral density of the HRV were analysed using the Fast Forier Transform algorithm. The appropriate statistical analysis of data was performed. LF density decreased at the anesthetized stage and increased at the insufflated stage in both groups (p 0.01). LP resulted in lower sympathetic activation than SP pneumoperitoneum (LF: 68.12 ń 6.56 nu vs. 78.26 ń 5.43 nu, p 0.01; HF: 24 ń 3.48 nu vs. 15.56 ń 2.54 nu, p ...

1111/2523

Tytuł oryginału: Various patterns of oral mucosa candidiasis treatment in HIV patients.
Tytuł polski: Sposoby leczenia kandydozy błony śluzowej jamy ustnej u chorych zakażonych HIV.
Autorzy: Macura Anna B., Bort Artur, Postawa-Kłosińska Barbara, Mach Tomasz
Źródło: Folia Med. Crac. 2002: 43 (1/2) s.69-77, il., tab., bibliogr. 19 poz., sum.
Sygnatura GBL: 304,697

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Jama ustna jest miejscem występowani różnych objawów u chorych zakażonych wirusem HIV. Jednym z najczęstszych jest kandydoza jamy ustnej. Może ona być pierwszym objawem choroby podstawowej. Długotrwała obserwacja wykazała, że leczenie antyretrowirusowe jest korzystne również w odniesieniu do tego oportunistycznego zakażenia, ponieważ zmniejsza zarówno liczbę nawrotów, jak i ich nasilenie. Jednakże przedłużenie czasu przeżycia pacjentów stwarza konieczność również przedłużenia leczenia przeciwgrzybiczego a także obserwacji jego skuteczności i stosowanych metod. Celem pracy była ocena wpływu leczenia antyretrowirusowego co najmniej trzema lekami na występowanie kandydozy jamy ustnej u chorych leczonych w oddziale szpitalnym lub Regionalnej Przychodni HIV/AIDS przy Katedrze i Klinice Chorób Zakaźnych Collegium Medicum UJ w Krakowie. Badaniem objęto 75 pacjentów z potwierdzonym zakażeniem HIV. Wykazano, obniżenie liczby grzybów w jamie ustnej u pacjentów poddanych leczeniu antyretrowirusowemu oraz zwiększenie wrażliwości grzybów na flukonazol.

Streszczenie angielskie: Oral cavity is the site of numerous HIV infection manifestations. Oral mucosa candidiasis is the most common one. It may be the earliest sign of the underlying disease. Long lasting observations give evidence that antiretroviral therapy is beneficial also in the cases of this opportunistic infection because it reduces both the number and severity of relapses, however, the prolongation of the patients' survival time creates the need of antifungal therapy prolongation, and thorough observation of its effectiveness and methods. We decided to analyze the influence of antiretroviral therapy with at least three drugs on the development of oral mucosa candidiasis in the out- and inpatietns of the Jagiellonian University Medical College Clinic of Infectious Diseases (or Outpatient Clinic) in Cracow. The study was carried out in 75 patietns with confirmed HIV infection. We have shown a decrease in the number of fungi present in the oral cavity in patients under antiretroviral treatment as well as higher susceptibiltiy to fluconazole.

1112/2523

Tytuł oryginału: Smooth-muscle progenitor cells of bone marrow origin contribute to the development of neointimal thickenings in rat aortic allografts and injured rat carotid arteries.
Autorzy: Religa Piotr, Bojakowski Krzysztof, Maksymowicz Michał, Bojakowska Maria, Sirsjo Allan, Gaciong Zbigniew, Olszewski Waldemar, Hedin Ulf, Thyberg Johan
Źródło: Transplantation 2002: 74 (9) s.1310-1315, il., tab., bibliogr. 30 poz.
Sygnatura GBL: 304,909

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: This study indicates that circulating progenitors of bone marrow orgin give to cells with smooth muscle-like properties during formation of neointimal thickenings in the arterial wall after allotransplantation and after balloon injury. A segment of abdominal aorta was transplanted from female F344 to male LEW rats, and the grafts were analyzed for male cells by using the SRY gene a marker. Immunostaining demonstrated that CD45-positive leukocytes made up 35-45 p.c. of the neointimal cells during the 8-week period eamined. Concurrently, up to 70 p.c. of the neointimal cells were of host origin, as shown by real-time polymerase chain reaction for the SRY gene (Y chromosome). This suggests that te neointima contained host cells also of noniflammatory character. Accordingly, many cells positive for smooth-muscle ŕ-actin were detected in this layer. To explore the possible bone marrow origin of allograft cells, female LEW rats were irradiated and substituted with bone marrow from male LEW rats. Subsequently, the animals received an aortic transplant from female F344 rats or were exposed to a balloon injury of the carotid artery. Immunostaining and real-time polymerase chain reaction confirmed the above findings, but the fractions of leukocytes and SRY-positive cells were lower in the carotids than in the allografts. Combined primed in situ labelin and immunostaining verified that not only inflammatory but also smooth muscle-like cells of male origin appeared in the vessel wall in both situations. These observations suggest that the smooth-muscle cells that participate in the development of neointimal lesions during vascular disease may, in part, originate from circulating progenitors.

1113/2523

Tytuł oryginału: Some aspects of hemostasis in CAPD patients treated with erythropoietin.
Autorzy: Małyszko Jolanta, Suchowierska Ewa, Małyszko Jacek S., Myśliwiec Michał
Źródło: Kidney Blood Press. Res. 2002: 25 (4) s.240-244, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,314

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Bleeding diathesis and simultaneous thrombotic complications may be seen in dialyzed patients. Erythropoietin may shift the precarious balance of the hemostatic system towards thrombosis. Thrombin activatable fibrinolysis inhibitor (TAFI) is a key protein linking coagulation and fibrinolysis. Methods: The effects of 3-month erythropoietin treatment on some hemostatic parameters - TAFI, fibrinolytic activity index (FAI), markers of ongoing coagulation (thrombin-antithrombin complexes and prothrombin fragments 1 + 2), marker of ongoing fibrinolysis (plasmin-antiplasmin complexes) and marker of endothelial cell injury (thrombomodulin) - were studied in 17 patients on continuous ambulatory peritoneal dialysis (CAPD). Patients on CAPD not treated with rHuEPO were also studied. Healthy volunteers served as a control group. 2,000 U erythropoietin was given subcutaneously three times a week. Commercially available kits were used to determine hemostatic parameters. Results: All the hemostatic parameters studied were significantly higher in CAPD patients when compared to the control group. All these hemostatic parameters except the plasmin-antiplasmin complexes did not differ significantly between patients before rHuEPO therapy and patients without rHuEPO. Erythropoietin therapy resulted in a significant decrease in plasmin-antiplasmin complexes, a significant rise in FAI after 3 months of drug administration, and a tendency to decrease the TAFI concentration and activity (after 1 month, p = 0.11 and p = 0.10, respectively; after 3 months p = 0.07 and p = 0.06, respectively)...

1114/2523

Tytuł oryginału: Lymphocytes in the structure of non-neoplastic goitre.
Autorzy: Biczysko Maciej, Stajgis Paweł, Żeromski Jan
Źródło: Pol. J. Pathol. 2002: 53 (2) s.73-81, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Mononuclear cells infiltrating the thyroid in various pathological states including those of the autoimmune etiology are common. The role of mononuclear onfiltrates in Hashimoto's goiter or Graves-Basedow disease is well understood. The present study was undertaken to analyze the immunophenotype of lymphocytes and the occurrence of antigen presenting cells in Graves-Basedow disease, toxic nodular goiter or simple goiter to compare these disease entitis with respect to their role in autoimmunization with thyroid antigens. Ninety-four thyroid specimens obtained from goiter patients undergoing surgery were studied. Cryostat sections were investigated with a panel of antibodies against antigens of lymphocyte differentiation using immunohistochemistry by APAAP. Semiquantitative technique was used to count the cells with positive reactivity in the visual field magnified 400 times under a light microscope. The structure of goiter varying in etiology contained all basic lymphocyte subpopulations and antigen presenting cells. T lymphocytes, especially CD3+, CD4+ cells were predominant, however the differences did not significantly correlate with any of the above mentioned patient group. There were also the signs of activation of infiltrate cells confirmed by a positive reaction with antibodies against class II MHC antigens. However, the presence of these cells did not correlate significantly with any of the analyzed groups. Furthermore, the expression of HLA class II antigens on epithelial calls of the thyroid was found in all groups, however much frequently in Graves-Basedow disease and toxic nodular goiter. This indicates a possibility of autoimmunization...

1115/2523

Tytuł oryginału: Degenerated lumbar intervertebral disc. A morphological study.
Autorzy: Lebkowski Wojciech J., Dzięcioł Janusz
Źródło: Pol. J. Pathol. 2002: 53 (2) s.83-86, il., bibliogr. 8 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli 45-64 r.ż.

Streszczenie angielskie: Acording to many investigators, lumbar disc herniation is formed by the nucleus pulposus displaced to the vertebral canal through the cracks in the annulus fibrosus as a final result of disc degeneration. Specimens obtained during 187 disc surgeries were studied to identify the morphological nature of various of disc herniation, especially protrusion and prolapse. In 29 p.c. of cases the herniated part was annulus fibrosus and in 21.5 p.c. cases the annulus fibrosus was seriously involved. Simultaneously 308 autopsy specimens were studied. On gross examination the signs of degeneration were advanced in 82, and strongly pronounced in 42. In 15 specimens, aged 40 years or older, fibre bundles werer reversed to normal convex orientation producing annulus fibrosus bulge. Microscopic examination showed pronounced myxomatous degeneration within the annulus fibrosus. In these circumstances, we belive that general opinion must be verified. Because in some herniations the nuclei pulposes are not involved and herniations are solety prolapses of annulus fibrosus. So, the lumbar intervertebral disc herniation should be classified on the basis of histological examinations as a herniation of nucleus pulposus, herniation of annulus fibrosus or as a mixed type herniation where both disc elements are involved.

1116/2523

Tytuł oryginału: Is it possible to identify siblings by studying bone marrow under a microscope? Two unusual cases of Gaucher disease.
Autorzy: Papla Bolesław, Machaczka Maciej, Skotnicki Aleksander B.
Źródło: Pol. J. Pathol. 2002: 53 (2) s.87-90, il., bibliogr. 8 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Two cases of Gaucher disease have been reported in siblings, in whom an unusual similar histological pattern permitted the pathologist (BP) to identify family relation of patients bearing different names.

1117/2523

Tytuł oryginału: Nephrogenic adenoma of the urinary bladder - a case report and review of the literature.
Autorzy: Chosia Maria, Świtała Jerzy, Domagała Wenancjusz
Źródło: Pol. J. Pathol. 2002: 53 (2) s.91-95, il., bibliogr. 26 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Nephrogenic adenoma is a rare benign of the urinary tract involving mainly the urinary bladder. The clinical manifestations, endoscopic signs and histopathological pattern of NA should be differentiated from a cancer, which may be a source of misinterpretation. Here we report a case of a 70-year--old man previously treated for papillary urothelial carcinoma. Six months later he developed a tumour, giving rise to a suspicion of recurrence. Histopathologically the tumour was diagnosed as nephrogenic adenoma. It is the first case of nephrogenic adenoma in Polish literature.

1118/2523

Tytuł oryginału: Rhabdomyosarcomatous (myoblastic ?) phentype of metastatic malignant melanoma. A case report.
Autorzy: Pasz-Walczak Grażyna, Jesionek-Kupnicka Dorota, Kubiak Robert, Biernat Wojciech, Sygut Jacek, Kordek Radzisław
Źródło: Pol. J. Pathol. 2002: 53 (2) s.97-100, il., bibliogr. 12 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli 19-44 r.ż.

Streszczenie angielskie: Melanoma may present a great variety of cell morphology and mimic virtually almost all other neoplasms, and metastatic lesions pose major diagnostic diffimulties. A typical immunoreactive profile of melanoma is: S-100p- and HMB-45-positivity, but these markers are not always present. Other new antigents for melanoma are NKIC3 and Anti-Melan-A (MART-1). This tumor may also present axpression of unspecific markers, as cytokeratin, CEA, EMA, desmin, NFP, GFAP, CD68 and actins. We present here unusual featres of two metastatic melanomas, showing the anaplastic pattern, imunopositive for desmin.

1119/2523

Tytuł oryginału: Reliability of endosonography in evaluation of anal fistulae and abscesses.
Autorzy: Sudoł-Szopińska I[wona], Gęśla J., Jakubowski W[iesław], Noszczyk W[ojciech], Szczepkowski M., Sarti D.
Źródło: Acta Radiol. 2002: 43 (6) s.599-602, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 301,623

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Objective: To assess the reliability of anal endosonography (AES) in the diagnosis of anal fistulae and abscesses. Material and Methods: 86 patients with different types of anal fistulae and abscesses were prospectively examined with a 7.0 MHz transducer. Type of anal fistulae, differentiation between simple and complex tracts, and location of their internal openings were defined. In 66 cases with permeable external opening hydrogen peroxide solution was introduced into the fistula tract. Reliability of AES was defined after surgical treatment of all cases. Results: 74 anal fistulae, including 43 transsphincteric, 11 intersphincteric, 6 suprasphincteric, 3 superficial, and 11 ano-vaginal were found on AES. 27 fistulae were complex, and 47 simple fistulae. In 10 patients a coexisting abscess was found; the remaining 12 abscesses were without any fistula. Surgery confirmed the type of anal fistula in patients (86.5 p.c.), and location of internal openings in 60 cases (81.1 p.c.). All abscesses were confirmed. Conclusion: AES showed high accuracy in diagnosing anal fistulae and abscesses.

1120/2523

Tytuł oryginału: Quercetin, apoptosis, heat shock.
Autorzy: Jakubowicz-Gil Joanna, Rzymowska Jolanta, Gawron Antoni
Źródło: Biochem. Pharmacol. 2002: 64 (11) s.1591-1595, il., tab., bibliogr. 33 poz.
Sygnatura GBL: 304,395

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The present study was designed to investigate the correlation between the expression level of Hsp27 and Hsp72 and induction of apoptosis in HeLa cells in response to quercetin treatment. Treatment of HeLa cells with quercetin or with 1 hr period of hyperthermia (42ř) increased the number of apoptotic cells. Inhibition of the expression of Hsp72 and Hsp27in tumour cells by anti-sense oligonucleotides, enhanced the induction of apoptosis by quercetin. Heat shock itself had little effect on apoptotic cell death in these cells, but when combinedwith quercetin treatment, caused a significant increase in the number of apoptotic cells. These results suggest that the reduction of Hsps expression in the HeLa cell line promotes the induction of apoptosis by quercetin.

1121/2523

Tytuł oryginału: Primary mediastinal large B-cell lymphoma is an important differential among mediastinal tumours.
Autorzy: Maryniak Renata K., Roszkowska-Purska Katarzyna
Źródło: Pol. J. Pathol. 2002: 53 (3) s.139-144, il., bibliogr. 29 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The study involved 51 patietns with histopathologically verified diagnoses of primary mediastinal large B-cell lymphoma (PMBL). Clinically they presented as the anterior mediastinal tumours, frequently with the superior vena cava syndrome (21 patietns) and usually (in 42 out of 51) without involvement of other locations. Morphologically the tumours were very pleomorphic. Histopathologically they were composed of germinal center cells, clear cells or polymorphic cells of various size and shape. However, there were also PMBLs composed of cells resembling Reed-Sternberg and Hodgkin cells and small cells. Various types of stromal sclerosis were evident in most biopsies. Due to lymphoma patterns mimicking other tumours with primary or secondary mediastinal location it was necessary to use a panel of immunocytochemical stains. CD20 staining proved to be indispensable for distinguishing PMBL. The patients were treated with CHOP or MEVA (V - VIII courses) followed by radiotherapy of the mediastinum (3600 - 4200cGy/t). Complete remission was obtained in 21 patients with overall survival ranging from 36 to 99 months. A total of 24 patients are alive, 3 of them live with persisting disease. One patient was lost to follow up, 26 died despite the treatment due to persistnece of mediastinal tumour or dissemination to distant organs.

1122/2523

Tytuł oryginału: Molecular aspects of bone healing and remodeling.
Autorzy: Szczęsny Grzegorz
Źródło: Pol. J. Pathol. 2002: 53 (3) s.145-153, tab., bibliogr. 136 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The process of fracture healing involves a complex series of coordinated cellular and molecular processes leading to removal of material contaminating the wound, angiogenesis validating restoration of disrupted microcirculation, and restoration of bone continuity by activation, proliferation and chemotaxis of bone progenitors from surrounding periosteum and endosterum. The regulation of various cell populations that orchestrate in this rocess depends on the biological effect evoked by cytokines and growth factors. The paper presents the state-of-the-art knowledge of the influence of growth factors and cytokines, namely TGF-á, BMP, IGF, PDGF, FGF, NO, IL-1, IL-6, IL-11 on the process of fracture healing.

1123/2523

Tytuł oryginału: A correlation between immunoexpression of CD44, ŕ-SMA and CD68+ cells in IgA-nephropathy and in mesangial proliferative IgA-negative glomerulonephritis.
Autorzy: Wągrowska-Danilewicz Małgorzata, Danilewicz Marian
Źródło: Pol. J. Pathol. 2002: 53 (3) s.155-162, il., tab., bibligor. 37 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Immunoperoxidase staining was carried out using monoclonal antibodies against CD44, ŕ-smooth muscle actin and CD68 on renal biopsy specimens from patients with IgA nephropathy (IgAN, n=16) and mesangial proliferative IgA-negative glomerulonephritis (MesProGN, n=15). As a control 10 biopsy specimens of the kidneys removed because of trauma were used. The results showed increase in glomerular CD44+ cells and CD68+ cells in IgAN biopsies as compared with controls and MesProGN. The intensity of glomerular ŕ-SMA was increased in IgAN and MesProGN as compared with controls, but no statistical differences were shown in glomerular expression of ŕ-SMA between IgAN and MesProGN. In both study groups there were significant positive correlations between glomerular CD44+ cells and CD68+ cells, as well as the intensity of ŕ-SMA immunostaining. The interstitial expression of CD44 and ŕ-SMA in IgAN patietns was significantly higher as compared with controls and MesProGN group, whereas the mean values of interstitial CD68+ cells did not differsignificantly in these glomerulopatheis. in both IgAN and MesProGN there were significant positive correlations between interstitial expression of CD44 and ŕ-SMA, between intensity of CD44 immunostaining and 68+ cells as well as interstitial volume. In conclusion, our study suggests possible role of CD44 interaction in macrophage recruitment and the development of interstitial fibrosis and glomerular sclerosis in both glomerulopathies.

1124/2523

Tytuł oryginału: Rudolf Virchow and presentation of his scientific achievement in Polish medical magazines in the 19th century and the beginning of 20th century.
Autorzy: Sabat Daniel, Dziembała Anna, Panasiewicz Małgorzata
Źródło: Pol. J. Pathol. 2002: 53 (3) s.163-168, il., bibliogr. 26 poz.
Sygnatura GBL: 301,852

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XIX wieku

Temat osobowy:

Virchow

Rudolf 1821-1902

Streszczenie angielskie: The development of pathological anatomy in the second half of the 19th century was mainly associated with the scientific activity of Rudolf Virchow. last year we celebrated the 180th birth anniversary and this year the 100th death anniversary of this outstanding man. The paper presents the scientific achievements of Rudolf Virchow as it was presented in Polish medical magazines in the 19th century and the beginning of 20th century. In 1858, his theory of cellular pathology became a basis for the understanding and fight against pathologic processes in living organisms. The theory also contributed to an increased interest in pathologic anatomy, especially histopathology and Virchow himself. in 1859 an extensive summary of the article, issued a year before, entitled "Cellular pathology based on physiolgoical and pathological tehroy of tissues" appeared in the subsequent issues of "Tygodnik Lekarski' ("Medical Weekly"). Also a translation of the XI chapter of "Cellular pathology..." devoted to nerve tissue appeared in the same magazine. this is the only treatise in Polish onteh most important work in the history of pathological anatomy of the 19th century. Three years later, in 1862, Włodzimierz Brodowski delivered to the students of Medical and Surgery Academy in Warsaw a lecture "Introduction to the lecture on pathological anatomy" which was later printed in "Pamiętnik Towarzystwa Lekarskiego Warszawskiego" ("diary of Warsaw Medical Association"). He presented the development of pathological anatomy along iwht the achievements of Virchow...

1125/2523

Tytuł oryginału: Silicoproteinosis of the lung in a 49-year-old man.
Autorzy: Papla Bolesław, Dubiel-Bigaj Magdalena, Malinowski Eugeniusz
Źródło: Pol. J. Pathol. 2002: 53 (3) s.169-171, il., bibliogr. 6 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: We report a rare case of silicosis, histologically corresponding to silicoproteinosis and tuberculosis, in a man working consecutively as a miner, blacksmith and founder. A microscopic study revealed deposits in alveoli, in which immunohistochemistry did not reveal surfactant (SP-A), that was present in the alveolar fluid in alveolar lipoproteinosis.

1126/2523

Tytuł oryginału: Ectopic thyroid tissue as a tumour of the heart - case report and review of the literature.
Autorzy: Chosia Maria, Waligórski Szymon, Listewnik Maria H., Wiechowski Seweryn
Źródło: Pol. J. Pathol. 2002: 53 (3) s.173-175, il., bibliogr. 14 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Here we report a case of a 48-year-old woman with ectopic thyroid tissue in the right ventricle. The tumour involved 1/3 of the ventricle and adhered to the myocardium. The lesion was removed completely and a bioprosthesis was placed due to tricuspid insufficiency, and then a pacemaker was implanted. The diagnosis was made upon examination of the postoperative tissue. The paper discusses embryological implications of the development of thyroid tissue in the heart. This case is first in the polish literature.

1127/2523

Tytuł oryginału: Primary undifferentiated small cell carcinoma (Merkel cell carcinoma) in a patient after heart transplantation - case report.
Autorzy: Maksymiuk Beata, Wygoda Andrzej, Zakliczyński Michał, Paleń Piotr
Źródło: Pol. J. Pathol. 2002: 53 (3) s.177-180, il., bibliogr. 17 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Here we report a case of a 48-year-old man after heart transplantation in whom two years after the procedure an appendage germ tumour was diagnosed and a year after its removal in the same area a primary undifferentiated small cell carcinoma (Merkel cell carcinoma) with metastases to the axillary lymph nodes was detected. The diagnosis of Merkel cell carcinoma was confirmed by immunohistochemical staining showing a typical perinuclea expression of cytokeratin 20 with the absence of reactivity with wide spectrum cytokeratin and the presence of neuroendocrine markers of neurone-specific enolase and chromogranin. Primary undifferentiated small cell carcinoma occurs more frequently in patients receiving immunosuppressive treatment as compared with a general population and occurs much more frequently in those below 50 years of age.

1128/2523

Tytuł oryginału: Mice and humans: chromosome engineering and its application to functional genomics.
Autorzy: Klysik Jan
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.553-569, il., bibliogr. [93] poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Functional modelling of human genes and diseases requires suitable mammalian model organisms. For its genetic malleability, the mouse is likely to continue to play a major role in defining basic genetic traits and complex pathological disorders. Recently, gene targeting tecyniques have been extended towards developing new engineering strategies for generating extensive lsions and rearrangements in mouse chromosomes. While these advances create new opportunities to address similar aberrations observed in human diseases, they also open new ways of scaling-up mutagensis projects that try to catalogue and annotate cellular functions of mammalian genes.

1129/2523

Tytuł oryginału: Molecular basis of inherited predispositions for tumors.
Autorzy: Lubiński Jan, Górski Bohdan, Kurzawski Grzegorz, Jakubowska Anna, Cybulski Cezary, Suchy Janina, Dębniak Tadeusz, Grabowska Ewa, Lener Marcin, Nej Katarzyna
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.571-581, il., tab., bibliogr. [63] poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: On the basis of literature data and own experience the authors review the current knowledge about the molecular basis of inherited predispositions for tumors. They hpothesize that in the near perspective 5-10 years studies using existing registry data/metarial and the latest novel tehcnology will allow the identification of the molecular background for the majority of hereditay cancers which will have normous practical consequences especially for the prevention of malignancies.

1130/2523

Tytuł oryginału: Recognition and repair of DNA-cisplatin adducts.
Autorzy: Woźniak Katarzyna, Błasiak Janusz
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.583-596, il., tab., bibliogr. [100] poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Anticancer activity of cisplatin (cis-diamminedichloroplatinum) is believed to result from its interaction with DNA. The drug reacts with nucleophilic sites in DNA forming monoadducts as well as intr-and interstrand crosslinks. DNA-cisplatin adducts are specifically recognized by several proteins. They can be divided into two classes. One constitutes proteins which recognize DNA damage as an initial step of the nucleotide excision and mismatch repari pathways. The other class contains proteins stabilizing cellular DNA-protein and protein-protein complexes, including non-histone proteins stabilizing cellular DNA-protein and protein-protein complexes, including non-histone proteins from the HMG (high-mibility-group) family. They specifically recognize 1,2-interstrand d(GpG0 and d(ApG) crosslinks of DNA-cisplatin adducts and inhibit their repair. Many HMG-domain proteins can function as transcription factors, e.g. UBF, an RNA polymerase I transcription factor, the mamalian testis-determining factor SRY and the human mitochondrial transcription factor mtTFA. Moreover, it seems that some proteins, which probably recognize DNA-cisplatin adducts non-specifically, e.g. actin and other nuclear matrix proteins, can disturb the the structural and functional organization of the nucleus and whole cell. The formation of complexes between DNA and proteins in the presence of cisplatin and the changes in the cell architecture may account for the drug cytotoxicity.

1131/2523

Tytuł oryginału: NTPase/helicase of Flaviviridae: inhibitors and inhibition of the enzyme.
Autorzy: Borowski Peter, Niebuhr Andreas, Schmitz Herbert, Hosmane Ramachandra S., Bretner Maria, Siwecka Maria A., Kulikowski Tadeusz
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.597-614, il., tab., bibliogr. [82] poz.
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are invovled in transcription and replication of viral single-stranded RNA genomes. The enzymatic acitivities of a NTPase/helicase were also detected in the carboxyl-terminal non-structural protein 3 (NS3) of members of the Flaviviridae family. The crucial role of the enzyme for the virus life cycle was demonstrated in knock out experiments and by using NTPase/helicase specific inhibitors. This makes the enzyme and attractive target for development of FLaviviridae-specific antiviral therapies. This review will summarize our knowledge about the function and structure of the enzyme, eupdate the spectrum of inhibitors of the enzymatic activities of the NTPase/helicase anddescribe the different mechanisms by which the compouneds act. Some of the compounds reviewed herein could show potential utility as antiviral agents atgainst Flaviviridae viruses.

1132/2523

Tytuł oryginału: Increased expression of ribosomal protein S2 in liver tumors, posthepactomized livers, and proliferating hepatocytes in vitro.
Autorzy: Kowalczyk Piotr, Woszczyński Marek, Ostrowski Jerzy
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.615-624, il., bibliogr. s. 622-624
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The ribosomal protein S2 (RPS2) is encoded by a gene from the highly conserved mammalian repetitive gene family LLRep3. It participates in aminoacyl-transfer RNA binding to ribosome, potentially affecting the fidelity of mRNA translation. These studies were designed to measure the expressiono f RPS2 during increased cell proliferation. Using Western and Northern blot analyses, we found that the levels of RPS2 protein and its corresponding mRNA were higher in mouse hepatocellular carcinoma, in mouse livers after one-third partial hepatectomy, and in serum-starved cultured hepatocytes following serum treatment. Our study shows that the increased expression of RPS2 correlated with increased cell proliferation. However, whether the altered expression of this protein reflects its involvement in cellular proliferationor represents an associated phenomena is still a key question that needs to be explored.

1133/2523

Tytuł oryginału: Optimisation of transfection conditions of CD34+ hematopoietic cells derived from human umbilical cord blood.
Autorzy: Ołdak Tomasz, Kruszewski Marcin, Machaj Eugeniusz Krzysztof, Gajkowska Agnieszka, Pojda Zygmunt
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.625-632, il., tab., bibliogr. s. 630-632
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

noworodki

Streszczenie angielskie: Human umbilical cord blood is frequently used as a source of transplantable hematopoietic cells and more recently as a target of gene therapy - a new approach for treatment of various disorders. The aim of our study was optimisation of the transfection conditions of cord blood-derived CD34+ hematopoietic cells. Mononuclear cells fraction was isolated from cord blood samples by density gradient centrifugation. Subsequently, CD34+ hematopoietic cells were separated on immunomagnetic MiniMACS columns. Pure population of CD34+ cells was incubated in a serum free medium supplemented with thrombopoietin, stem cell factor and Flt-3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene. We studied the influence of various pulse settings and DNA concentrations on teh transfection efficiency, measured by flow cytometry as the fluorescence of target cells due to the expression of EGFP. The optimal settings were as follows: 4 mm cuvette, 1600 ćF, 550 V/cm, and 10 ćg of DNA per 500 ć1. With these settings we obtained a high transfection frequency (41.2 p.c.) without a marked decrease of cell viability. An increase of the pulse capacitance and/or of DNA concentration resulted in a greater electroporation efficiency, but also in a decrease of cell viability. In conclusion, the results described here allow one to recommend electroporation as an efficient method of gene delivery into CD34+ hematopoietic cells derived from human umbilical cord blood.

1134/2523

Tytuł oryginału: Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.
Autorzy: Waszkowiak Aneta, Frąckowiak Danuta, Wiktorowicz Krzysztof, Miyake Jun
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.633-641, il., bibliogr. s. 640-641
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. this suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. the absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establishe the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.

1135/2523

Tytuł oryginału: Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.
Autorzy: Lityńska Anna, Przybyło Małgorzata, Pocheć Ewa, Laidler Piotr
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.643-650, il., bibliogr. s. 649-650
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the ŕ2, ŕ5 and á1 integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. in contrast, adhesion of cancer cells was not inhibited by specific antibodies to the á1 integrin subunit. Antiboedies to ŕ3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that ŕ3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.

1136/2523

Tytuł oryginału: The effect of Arg209 to Lys mutation in mouse thymidylate synthase.
Autorzy: Cieśla Joanna, Gołos Barbara, Wałajtys-Rode Elżbieta, Jagielska Elżbieta, Płucienniczak Andrzej, Rode Wojciech
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.651-658, il., tab., bibliogr. s. 657-658
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Mouse thymidylate synthase R209K (a mutation corresponding to R218K in Lactobacillus casei), overexpressed in thymidylate synthase-deficient Escherichia coli strain, was poorly soluble and with only feeble enzyme activity. The mutated protein, incubated with FdUMP and N5,10 -methylenetetrahydrofolate, did not form a complex stable under conditions of SDS/polyacrylamide gel electrophoresis. The reaction catalyzed by the R209K enzyme (studied in a crude extract), compared to that catalyzed by purified wild-type recombinant mouse thymidylate synthase, showed the Km value for dUMP 571-fold higher and Vmax value over 50-fold (assuming that the mutated enzyme constituted 20 p.c. of total crude extract protein) lower. Thus the ratios kcat, R209K/kcat, 'wild' and (kcat, R209K/Km, R209K**dUMP)/(kcat, 'wild'/Km, 'wild'**dUMP) were 0.019 and 0.000032, respectively, documenting that mouse thymidylate synthase R209, similar to the corresponding L. casei R218, is essential for both dUMP binding and enzyme reaction.

1137/2523

Tytuł oryginału: Effect of An tracts within the UP element proximal subsite of a model promoter on kinetics of open complex formation by Escherichia coli RNA polymerase.
Autorzy: Kolasa Iwona K., Łoziński Tomasz, Wierzchowski Kazimierz L.
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.659-669, il., tab., bibliogr. s. 667-669
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: In the open transcription complex (RPo), Escherichia coli RNA polymerase sigma70 and ŕ subunits are known to be in contact with each other and with the promoter region overlapping the -35 hexamer and the proximal part of the UP element. To probe the effect of AnDNA bending tracts in this region on initiation of transcription, kinetics of the formation of RPo by Escherichia coli RNA polymerase at two groups of synthetic consensus-like promoters bearing single DNA bending tracts (i) A5 within the proximal subsite region of the UP element (promoters Pk and Pl) and (ii) A5 (Pg) or A8 (Pm) in the region including the downstream end of the proximal UP subsite and the -35 consensus hexamer was studied in vitro using the fluorescence-detected abortive initiation assay. The kinetic data obtained demonstrate that the overall second-order rate constant Ka of RPo formation is: (i) by almost one order of magnitude larger at Pk and Pl, relative to that at a control unbent promoter, and mainly due to a higher value of the equilibrium constant, k1, of the initial closed complex; and (ii) several-fold smaller at Pg and Pm owing to a strongly decreased value fo k1. For Pm, the latter parameter was found to be dependent exponentially on four Mgý+ ions, as compared with the seven ions remaining in equilibrium with the initial closed complex at the parent Papromoter. This indicates that promoter region bearing a stiff A8úT8 fragment of B'-DNA forms a smaller number of ionic contacts with the ŕ subunit. These findings provide a new insight to and supprot the present model of interactions between RNA polymerase ŕ and sigma70 subunits with the proximal UP subsite and the -35 region of promoters.

1138/2523

Tytuł oryginału: Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues.
Autorzy: Stachelska Alicja, Wieczorek Zbigniew, Ruszczyńska Katarzyna, Stolarski Ryszard, Pietrzak Monika, Lamphear Barry J., Rhoads Robert E., Darżynkiewicz Edward, Jankowska-Anyszka Marzena
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.671-682, il., tab., bibliogr. s. 679-682
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Translation initiation factor eIF4E binds the m**7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70 p.c. of genes express mRNAs with an unusual cap structure containing m3ý,ý,**7 G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m**7 GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m**7 G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m**7 G- and m3ý,ý,**7 G-containing caps (Kas 2x10**6 M**-1 to 7x10**6 M**-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of M**7 G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 ph units than that of eIF4E from human erythrocytes. these results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.

1139/2523

Tytuł oryginału: Functional reconstitution of Ral-binding GTPase activating protein, RLIP76, in proteoliposomes catalyzing ATP-dependent transport of glutathione conjugate of 4-hydroxynonenal.
Autorzy: Sharma Rajendra, Sharma Abha, Yang Yusong, Awasthi Sanjay, Singhal Sharad S., Zimniak Piotr, Awasthi Yogesh C.
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.693-701, il., bibliogr. s. 700-701
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Earlier studies from our laboratories have shown that RLIP76, a previously described Ral-binding GTpase activating protein (Jullien-Flores et al., 1995, J. Biol. Chem. 270: 22473), is identical with the xenobiotic transporter DNP-SG ATPase, adn can catalyze ATP-dependent transport of glutathione-conjugates as well as doxorubin (Awasthi et al., 2000, Biochemistry, 39: 9327). We have now reconstituted purified bacterially expressed RLIP76 in proteoliposomes, and have studied ATP-dependent uptake of the glutathione conjugate of 4-hydroxynonenal (GS-HNE) by these vesicles. Results of these studies show that RLIP76 reconstituted in proteoliposomes catalyzes ATP-dependent transport of GS-HNE against a concentration gradient. The transport of GS-HNE is saturable with respect to ATP as well as GS-HNE with Km values of 1.4 mM and 2.5 ćM, respectively. These studies demonstrate that RLIP76 mediates active transport of GS-HNE, adn are consistent with our previous work showing that RLIP76-mediated efflux of GS-HNE regulates the intracellular concentration of 4-HNE and thereby affects 4-HNE mediated signaling.

1140/2523

Tytuł oryginału: The effects of the interaction of myosin essential light chain isoforms with actin in skeletal muscles.
Autorzy: Nieznańska Hanna, Nieznański Krzysztof, Stępkowski Dariusz
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.709-719, il., tab., bibliogr. 717-719
Sygnatura GBL: 303,116

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

króliki

Streszczenie angielskie: In order to compare the ability of different isoforms of myosin essential light chain to interact with actin, the effect of the latter protein on the proteolytic susceptibility of myosin light chains (MLC-1S and MLC-1V - slow specific and same as ventricular isoform) from slow skeletal muscle was examined. Actin protects both slow muscle essential light chain isoforms from papain digestion, similarly as observed for fast skeletal muscle myosin (Nieznańska et al., 1998, Biochim. Biophys. Acta 1383: 71). The effect of actin decreases as ionic strength rises above physiological values for both fast and slow skeletal myosin, confirming the ionic character of the actin - essential light chain interaction. To better understand the role of this interaction, we examined the effect of synthetic peptide spanning the 10-amino-acid n-terminal sequences of myosin light chain 1 from fast skeletal muscle (MLC-1F0 (MLCFpep: KKDVKKPAAA), MLC-1S (MLCSpep: KKDVPVKKPA) and MLC-1V (MLCVpep: KPEPKKDDAK) on the myorfibrillar ATPase of fast and slow skeletal muscle. In the presence of MLCFpep, we observed an about 19 p.c. increase, and in the presence of MLCSpepabout 36 p.c. increase, in the myofibrillar ATPase activity of fast muscle. On the other hand, in myofibrillar preparations from slow skeletal muscle, MLCSpep as well as MLCVpep caused a lowering of the ATPase activity by about 36 p.c. The above results suggest that MLCSpep induces opposite effects on ATPase activity, depending on the type of myofibrils, but not through its specific N-terminal sequence - which differs from other MLC N-terminal peptides...

1141/2523

Tytuł oryginału: Comparison of serological specificity of anti-endotoxin sera directed against whole bacterial cells and core oligosaccharide of Escherichia coli J5-tetanus toxoid conjugate.
Autorzy: Łukasiewicz Jolanta, Jachymek Wojciech, Niedziela Tomasz, Malik-Gębicka Małgorzata, Dzieciątkowska Monika, Lugowski Czesław
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.721-734, il., bibliogr. s. 732-734
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The rough mutants of Gram-negative bacteria are widely used to induce protective antisera but the nature of the target epitope for such antibodies is not precisely defined. Endotoxin is one of several antigens present on the surface of bacterial cells, which are able to elicit specific antibodies. We studied the specificity of antibodies produced against a conjugate of E. coli J5 endotoxin core oligosaccharide with tetanus toxoid. The use of chemically defined antigen for immunisation excludes the possibility of production of antibodies against otehr cell surface antigens. A comparison of this monospecific anti-endotoxin serum whith antiserum against E. coli J5 whole cells was performed in order to distinguish the role that endotoxin core oligosaccharide plays in the itneraction with humoral host defences form that of other potentially important Gram-negative bacterial surface antigens. The reactivity of both sera with smooth and rough lipopolysaccharides was determined in ELISA, immunoblotting and by flow cytometry. BOth antisera reacted with similar specificity with most lipopolysaccharides of identical or related core type. Less distnct reactions with endotoxins of antibacterial serum in comparison with the anti-conjugate serum were found in all serological tests. LPS of E. coli O100 that showed the strongest reactions with both sera was used to stimulate IL-6, TNFŕ and nitric oxide production by the J-774A.1 cell line. BOth sera were used to inhibit that stimulation and no inhibitroy effects of the examined sera in comparison with non-immune serum were observed.

1142/2523

Tytuł oryginału: Steroid signal transduction activated at the cell membrane: from plants to animals.
Autorzy: Marcinkowska Ewa, Więdłocha Antoni
Źródło: Acta Bioch. Pol. 2002: 49 (3) s.735-745, il., tab., bibliogr. s. 742-745
Sygnatura GBL: 303,116

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Steroid hormones in plants and in animals are very important for physiological and developmental regulation. In animals steroid hormones are recognized by nuclear receptors, which transcriptionally regulate specific target genes following binding of the ligand. In addition, numerous rapid effects generated by steroids appear to be mediated by a mechanism not depending on the activation of nuclear receptors. Although the existence of separate membrane receptors was postulated many years ago and hundreds of reports supporting this hypothesis have been published, no animal membrane steroid recepotr has been cloned to date. Meanwhile, a plant steroid receptor from Arabidopsis thaliana has been identified and cloned. It is a transmembrane protein which specifically recognizes plant steroids (brassinosteroids) at the cell surface and has a serine/threonine protein kinase activity. It seems that plants ahve no intracellular steroid receptors, since there are nog enes homologus to the family of animal nuclear steroid receptors in the genome of A. thaliana. Since the reason of the rapid responses to steroid hormones in animal cells still remains obscure we show in this article two possible explanations of this phenomenon. Using 1,25-dihydroxyvitamin D3 as an example of animal steroid hormone, we review results of our and of otehr groups concerdatn with the hypothesis of membrane steroid receptors. We also review the results of experiemnts performed with ovarian hormones, that led their authors to the hypothesis explaining rapid steroid actions without distinct membrane steroid receptors...

1143/2523

Tytuł oryginału: Proteus syndrome: a case with clonal chromosome aberration.
Autorzy: Gieruszczak-Białek D[orota], Illiszko M[ariola], Mikołajczyk W[iesław], Skórka A[gata], Korniszewski L[ech]
Źródło: Genet. Couns. 2002: 13 (4) s.427-432, il., bibliogr. 19 poz.
Sygnatura GBL: 313,584

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Proteus syndrome is a disorder characterized by overgrowth of multiple tissues, connective tissue nevi, epidermal nevi and hyperostoses with asymmetric involvement. The clinical expression of the disorder is extremal variable. Molecular pathogenesis of the syndrome is unknown but it is hypothesized that it resulted from a somatic alternation of a gene leading to mosaic effects that would be lethal if the mutation was carried in nonmosaic fashion, and this may explain the variability among patients. We report a new case who presented at birth with asymmetric hypertrophy of the bones and soft tissues of fingers and a tumor of the chest. Cytogenetic analysis of the excised tumor revealed clonal chromosome aberration: mos46, XY, add(9) (p13) [5]/46,XY[30]. During follow up tumors of the rectum and urinary bladder were diagnosed.

1144/2523

Tytuł oryginału: Modulation of melanocortin-induced changes in spinal nociception by ć-opioid receptor agonist and antagonist in neuropathic rats.
Autorzy: Starowicz Katarzyna, Przewłocki Ryszard, Gispen Willem Hendrik, Przewłocka Barbara
Źródło: Neuroreport 2002: 13 (18) s.2447-2452, il., tab., bibliogr. 25 poz.
Sygnatura GBL: 313,357

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Co-localization of opioid and melanocortin receptor expression, especially at the spinal cord level in the dorsal horn and in the gray matter surrounding the central canal led to the suggestion that melanocortins might play a role in nociceptive processes. In the present studies, we aimed to determine the effects of melanocortins, administered intrathecally, on allodynia, and to ascertain whether there is an interaction between opioid and melanocortin systems at the spinal cord level. Neuropathic pain was induced by chronic constriction injury (CCI) of the right sciatic nerve in rats. Tactile allodynia was assessed using von Frey filaments, while thermal hyperlagesia wa evaluated in cold water allodynia test. In the present experiments, melanocortin receptor antagonist, SHU9119 was much more potent than ć-opioid receptor agonist, morphine after their intrathecal (i.th.) administration in neuropathic rats. SHU9119 alleviated allodynia in a comparable manner to DAMGO, a selective and potent ć-opioid receptor agonist. Administration of melanocortin receptor agonist, melanotan-II (MTII) increased the sensitivity to tactile and cold stimulation. Moreover, we demonstrated that the selective blockade of ć-opioid receptor by cyprodime (CP) enhanced antiallodynic effect of SHU9119 as well as pronociceptive action of MTII, whereas the combined administration of ć receptor agonist (DAMGO) and SHU9119 significantly reduced the analgesic effect of those ligands. DAMGO also reversed the proallodynic effect of melanocortin receptor agonist...

1145/2523

Tytuł oryginału: Luminance and chromatic contrast sensitivity in dyslexia: the magnocellular deficit hypothesis revisited.
Autorzy: Bednarek Dorota B., Grabowska Anna
Źródło: Neuroreport 2002: 13 (18) s.2521-2525, il., bibliogr. 29 poz.
Sygnatura GBL: 313,357

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The hypothesis of a magnocellular channel deficit in dyslexia was tested. Subjects were 10-year-old dyslexics and normal readers. Psychophysical thresholds for luminance and chromatic contrasts were estimated using black and white and red and green sinusoidal gratings of various spatial frequencies, presented in static and dynamic conditions (drift and reversal). Significant group differences were found for luminance contrast, with a higher sensitivity in dyslexics. No group differences were obtained for chromatic contrast. High luminance sensitivity correlated with low reading and writing skills. The typical finding of an increase contrast sensitivity to low spatial frequency gratings, due to their dynamic presentations, was absent in dyslexics. The results provide support for the magnocellular deficit hypothesis. The pattern of this deficit, however, is much more complex than that emerging from previous research.

1146/2523

Tytuł oryginału: Exercise increases mRNA levels for adhesion molecules N-CAM and LI correlating with BDNF response.
Autorzy: Macias Matylda, Fehr Susanne, Dwornik Anna, Sulejczak Dorota, Wiater Maciej, Czarkowska-Bauch Julita, Skup Małgorzata, Schachner Melitta
Źródło: Neuroreport 2002: 13 (18) s.2527-2530, il., bibliogr. 27 poz.
Sygnatura GBL: 313,357

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: In situ hybridization was used to evaluate whether long-term moderate locomotor exercise, which up-regulates BDNF and TrkB levels in the spinal gray matter of the adult rat, similarly influences the expression of the cell adhesion molecules N-CAM and LI. Exercise doubled the level of N-CAM mRNA hybridization signal in the lumbar spinal gray. The increase in LI mRNA was less consistent. N-CAM mRNA levels slightly increased in the white matter. BDNF mRNA levels also increased in cells of the ventral horn and the white matter due to the exercise. Theses results suggest that exercise-induced rearrangements of the spinal network involve N-CAM, LI and BDNF, crucial in different aspectcs of synaptic plasticity and synapse formation.

1147/2523

Tytuł oryginału: Un cas de rcupration partielle de I'audition aprs I'ablation d'un neurinome de I'angle pontocrbelleux.
Tytuł angielski: Partial hearing recovery after cerebello-pontine angle neurinoma removal.
Autorzy: Bochenek W., Hatliński G., Kukwa A[ndrzej], Krajewski R.
Źródło: Rev. Laryngol. Otol. Rhinol. 2002: 123 (3) s.199-201, il., bibliogr. 5 poz., sum.
Sygnatura GBL: 300,240

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: A cause of a 44 year-old women farmer with a total deafness and lack of vestibular function on the left side operated upon the cerebello-pontine cystic neuroma 2.5 x 1.5 cm large via the retromastoidal craniectomy is presented. Despite of the negative audiological tests' results the cochlear nerve flatten on the tumor was cautiously separated. Some time after surgery, she observed "a partial recovery of hearing" in the operated ear confirmed by the tonal (mean 0.5-2 kHz 36 dBHL) vocal SRT 70 dB), BERA and EOAEs tests. The paralysis of the left vestibular function did not recover. In consecutive audiogram a notch at 6 kHz 15 dB deep appeared in the operated ear and one can presume an increased vulnerability of this ear to the noise of the agricultural machines used by then by the patient.

1148/2523

Tytuł oryginału: Influence du lanrotide sur la concentration sreique de prolactine chez les patients atteints de tumeurs somato-lactotropes.
Tytuł angielski: Effect of lanreotide on prolactin level in patients with pituitary mixed tumors.
Autorzy: Wasko R., Sawicka J., Stachowiak C., Kozak W., Junik R., Sowiński J.
Źródło: Ann. Endocrinol. 2002: 63 (6 cz. 1) s.532-535, il., bibliogr. 12 poz., sum.
Sygnatura GBL: 301,225

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Acromegaly is a disease caused by a pituitary tumor (somatotropinoma) or by ectopic secretion of GH or IGF-1. About 15 p.c. of tumors secrete not only GH but PRL as well. Last time a lanreotide and an octreotide (the somatostatine analogues) are useful in the therapy of acromegaly. We observed the influence of the lanreotide on GH and prolactin. We noticed that the lanreotide caused not only serum level reduction of a growth hormone but also prolactine in patients with mixed pituitary tumors.

1149/2523

Tytuł oryginału: The role of solar ultraviolet irradiation in zoster.
Autorzy: Żak-Prelich M., Borkowski J. L., Alexander F., Norval M.
Źródło: Epidemiol. Infect. 2002: 129 (3) s.593-597, il., tab., bibliogr. 16 poz.
Sygnatura GBL: 313,292

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Ultraviolet radiation (UVR) suppresses many aspects of cell-mediated immunity but it is uncertain whether solar UV exposure alters resistance to human infectious diseases. Varicella-zoster virus (VZV) causes varicella (chickenpox and zoster in a defined Polish population over 2 years was recorded and ground level solar UV was measured daily. There was a significant seaonality of UVR. Evidence of seasonal variation was found for all zoster cases and for zoster in males, with the lowest number of cases in the winter. The number of zoster cases with lesions occurring on exposed body sites (the face) demonstrated highly significant seasonality with a peak in July/August. Seasonal models for UVR and zoster cases was found in Marh and the minimum in August/ September, probably explained by the respiratory spread of VZV. It is tempting to speculate that the increase in solar UVR in the summer could induce suppression of cellular immunity, thus contributing to the corresponding rise in the incidence of zoster.

1150/2523

Tytuł oryginału: Lichtenstein repair for incarcerated groin hernias.
Autorzy: Wysocki Andrzej, Poźniczek Marek, Krzywoń Jerzy, Strzałka Marcin
Źródło: Eur. J. Surg. 2002: 168 (8/9) s.452-454, bibliogr. 25 poz.
Sygnatura GBL: 300,370

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Objective: To evaluate the use of Lichtenstein tension-free repair for incarcerated groin hernias. Design: Prospective consecutive series. Setting: Tertiary academic surgery department, Poland. Subjects: 27 patients whose incarcerated groin hernias were operated on between November 1997 and June 2001. Intervention: Tension-free hernia repair by the Lichtenstein technique. Main outcome measures: Morbidity, recurrence rates and mortality. Results: There were 2 postoperative deaths unrelated to implantation of the mesh (myocardial and cerebral infarction). In 1 patients a subcutaneous fluid collection was noted and was auccessfully treated by evacuation of the fluid. The remaining 24 patients had no complications during follow-up (mean 18 months, range 2-42 months). Conclusion: Monofilament polypropylene mesh can be successfully used not only in elective operations, but also in emergency operations for incarcerated inguinal hernias.

1151/2523

Tytuł oryginału: What are GPs in need of for approaching alcohol-related problems?
Autorzy: Rodriguez-Martos Dauer Alicia
Źródło: Probl. Med. Rodz. 2002: 4 (1) s.6-11, tab., bibliogr. 13 poz.
Sygnatura GBL: 313,528

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: To face alcohol-related problems at a Primary Care setting isn't easy because of many barriers. Among them, an insufficient training negatively influences the perceived self-efficacy and the therapeutic commitment. This article recalls the WHO recommendations for its adoption by faculties for general practitioners and suggests the key components (knowledge, skills and attitudes) and strategies (didactic lectures, practical training and promotion of attitudes) for training programmes aimed at general practitioners. Some support materials and the promotion of an easy "4-A plan" (ask, assess, advice, assistanticipate) are suggested as contents of the training programme, whereas the learner-centred principle and the "training trainers" model are strongly recommended for the programme's design.

1152/2523

Tytuł oryginału: Suitability of selected markers for indentification of elements of the Intestinal Nervous System (INS).
Autorzy: Dzienis-Koronkiewicz E[wa], Dębek W[ojciech], Sulkowska M[ariola], Chyczewski L[ech]
Źródło: Eur. J. Pediatr. Surg. 2002: 12 (6) s.397-401, il., bibliogr. 19 poz., rs., res., Zsfg
Sygnatura GBL: 312,942

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dzieci 6-12 r.ż.

Streszczenie angielskie: The possibility of identifying and characterising elements of the enteric nervous system based on their contents of cathepsin D, chromogranin A, neuronal specific enolase and S-100 protein was studied in colorectal specimens (operative full-thickness, seromuscular and mucosomuscular biopsies) obtained from 15 children, aged 2 days to 10 years. Nine patients suffered from Hirschsprung's disease, and two from chronic constipation. Four neonates with imperforate anus or meconium ileus composed the control group. All markers were identified immunohistochemically by antibodies against human antigens with appropriate detection methods. Chromogranin A staining was not always adequate to identify all neuronal cell bodies and other nervous elements. However, it proved superior to the other methods in the depiction of neuroendocrine cells in the intestinal mucosa. Cathepsin D antibodies stained normal and abnormal neural cells with different intensity; nerve fibres were not stained. This marker did not allow an unequivocal differentiation of ganglion cells from macrophages within the submucosa; the latter exhibited exceptionally strong marking and in some cases represented the predominant elements in this area. Neuronal specific enolase was distinctly expressed in nerve cells and fibres of the intestinal wall. Atrophic and hypoplastic features could be identified, suggesting that this method may give some insight into functional aspects. Continuous connections between ganglions were also observed. S-100 protein antibodies resulted in a negative image of unstained ganglion cells surrounded...

1153/2523

Tytuł oryginału: Synthesis and pharmacological activity of new carbonyl derivatives of 1-aryl-2-iminoimidazolidine. P. 3: Synthesis and pharmacological activity of 1-aryl-5,6(1H)dioxo-2,3-dihydroimidazol[1,2-a]imidazoles.
Autorzy: Matosiuk Dariusz, Fidecka Sylwia, Antkiewicz-Michaluk Lucyna, Dybala Izabela, Kozioł Anna E.
Źródło: Eur. J. Med. Chem. 2002: 37 (10) s.845-853, il., tab., bibliogr. 20 poz.
Sygnatura GBL: 312,629

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Synthesis and pharmacological activity of 1-aryl-5,6(1 H)dioxo-2,3-dihydroimidazo[1,2-a]imidazoles (D) are presented. The title compounds were obtained from 1-aryl-2-iminoimidazolidines (1) by cyclization reaction with oxalic acid derivatives-ethyl ester (2) or chloride (3). They were tested for pharmacological activity in animal and binding assay tests. With moderate acute toxicity (LD50 ~ 200 mg kg**-1, i.p.), they exhibited significant and serotonergic activities as results of the `writhing' and the `hot plate' tests indicated, and reduced number of `head twitch' episodes after 5-HTP (5-hydroxytryptophan) administration. Reversion of the antinociception produced in the `writhing' test by small dose of naloxon (5 mg kg**-1) can suggest an opioid-like mechanism of their analgesic activity. The probable receptor inhibition mechanism of their analgesic and serotonergic activity was confirmed in the binding assay tests (by radioligand displacement) toward the opioid ć and serotonin 5-HT2 receptors. Additionally, they exhibited affinity toward the benzodiazepine (BZD) receptor as well, although in behavioral tests compounds did not produce any clear depressive effect on the central nervous system (CNS) of mice. Simple chemical structure of the title compounds, in comparison to other carbonyl derivatives of 1-aryl-2-iminoimidazolidine presented in this series of papers, underline very important role both of a hydrophobic moiety (aromatic ring) and polar groups (hydrogen-bond acceptors) in the serotonin receptor interaction. The coexistence of opioid-like, serotonergic and BZD receptor inhibition activity can be very interesting and can lead to creation of the novel group of antidepressants.

1154/2523

Tytuł oryginału: Synthesis, antiprotozoal and anticancer activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles.
Autorzy: Andrzejewska Mariola, Ypez-Mulia Lilian, Cedillo-Rivera Roberto, Tapia Amparo, Vilpo Leena, Vilpo Juhani, Kazimierczuk Zygmunt
Źródło: Eur. J. Med. Chem. 2002: 37 (12) s.973-978, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 312,629

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The synthesis of several halogenated benzimidazoles substituted in position 2 with trifluoromethyl, pentafluoroethyl and 2-thioethylaminodimethyl group is reported. Antiprotozoal and anticancer activity of series of newly synthesized and previously obtained compounds was studied. All of tested bezimidazoles showed remarkable antiprotozoal activity against Giardia intestinalis, Entamoeba histolytica and Trichomonas vaginalis. Of the studied collection of halogenated benzimidazoles the most anticanceractive was the 5,6-dichloro-2-pentafluoroethyl compound, particularly against breast and prostate cancer cell lines.

1155/2523

Tytuł oryginału: Is acute hepatitis B still a nosocomially-acquired infection in Poland? A case-controlled study of transmission routes in a hospital sample.
Autorzy: Chlabicz Sławomir, Grzeszczuk Anna, Pytel Barbara, Prokopowicz Danuta
Źródło: Scand. J. Infect. Dis. 2002: 34 (11) s.808-810, tab., bibliogr. 9 poz.
Sygnatura GBL: 305,538

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of this study was to evaluate the role of medically linked risk factors for acute HBV infection in the Podlasie region of North-Eastern Poland. The records of 107 adult patients and 214 age- and sex-matched controls hospitalized in the Department of Infectious Diseases, Białystok, Podlasie Region during the period 1997-2000 with the diagnosis of acute hepatitis B were reviewed. On multivariate analysis, household contact with hepatitis B [odds ratio (OR) = 18.0, 95 p.c. confidence interval (CI) 2.0 - 165.5] and a history of injections (OR = 2.7, 95 p.c. CI 1.5 - 4.7) in the preceding 6 months were independently associated with acute HBV infection. In conclusion, our data indicate that despite a significant decline in the incidence of hepatitis B, injections are still involved in the spread of HBV in Poland.

1156/2523

Tytuł oryginału: Synthesis and antitumor activity of novel 2-amino-4-(3,5,5-trimethyl-2-pyrazolino)-1,3,5-triazine derivatives.
Autorzy: Brzozowski Z., Sączewski F.
Źródło: Eur. J. Med. Chem. 2002: 37 (9) s.709-720, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 312,629

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: The syntheses and antitumor activities of novel 2-amino-4-(3,5,5-trimethyl-2-pyrazolino)-1,3,5-triazine derivatives 4-38 are described. All the comppounds prepared were screened at the National Cancer Institute (NCI) for their activities against a panel of 60 tumor cell lines and relationships between structure and antitumor in vitro are discussed. The triazines 11, 16, 20, 23, 23 and 34-38 exhibited modesr or fairly high activity against one or more human tumor cell lines. Prominent compound with remarkable activity (log GI50, -80- - 5.00) to all investigated cell lines highly potent (log GI50 -8.00- - 7.64) against some cell lines of Laukemia (CCRF-CEM, K-562, RPMI-8226, SR), CNS Cancer (SF-539) and breast Cancer (T-47D) was 2-[2-amino-4-(3,5,5-trimethyl-2-pyrazolino)-1,3,5-triazin-6-yl]-3-(5-nitro-2-thienyl)acrylonitrile (25).

1157/2523

Tytuł oryginału: Synthesis and pharmacological activity of new carbonyl derivatives of 1-aryl-2-iminoimidazolidine. P. 2: Synthesis and pharmacological activity of 1,6-diaryl-5,7(1H)dioxo-2,3-dihydroimidazo[1,2-ŕ][1,3,5]triazines.
Autorzy: Matosiuk Dariusz, Fidecka Sylwia, Antkiewicz-Michaluk Lucyna, Lipkowski Janusz, Dybala Izabela, Koziol Anna E.
Źródło: Eur. J. Med. Chem. 2002: 37 (9) s.761-772, il., tab., bibliogr. 12 poz.
Sygnatura GBL: 312,629

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: Synthesis and pharmacological activity of 1,6-diaryl-5,7 (1H)dioxo-2,3-dihydroimidazo-[1,2-ŕ][1,3,5]triazines (C) are presented. The title compounds were obtained from 1-aryloimidazolinurea derivatives in cyclization reaction with difunctional carbonyl reagents - phosgene (method I) or carbonyldiimidazole (CDI) (method II). Their molecular structures were confirmed by the X-ray analysis of 1-phenyl-6-(4-chlorophenyl)-5,7(1H)-dioxo-2,3-dihydroimidazo[1,2-ŕ][1,3,5]triazine C2) crystals. Compounds C exhibited significant depressive action on the central nervous system (CNS) of the laboratory animals, correlated with very low acute toxicity (LD50 2000 mg kg**-1 i.p.), and showed antinociceptive activity in behavioural models. Reversion of this effect by small dose of naloxone (5 mg kg**-1) can suggest opioid-like mechanism of antinociception produced by these and other carbonyl derivatives of 1-aryl-2-iminoimidazolidine. Additionally, an effect on the serotonin neurotransmission pathway was also observed. The receptor mechanism of activity for investigated compounds was confirmed only for the opioid ć receptor in binding affinity assay test. Same tests performed for the serotonin 5-HT2 and benzodiazepine BZD receptors showed no affinity for tested compounds. The opioid-like and serotonergic activities are similar to these described earlier for chain carbonyl 1-aryl-2-iminoimidazolidine derivatives containing urea moiety, mainly due to similar chemical structure, although compounds C are not able to adopt any of the higher energy confirmations of urea derivatives...

1158/2523

Tytuł oryginału: Low-affinity kainate receptor-mediated events reduce the protective activity of phenobarbital and diphenylhydantoin against maximal electroshock in mice.
Autorzy: Borowicz K. K., Zadrożniak M., Czuczwar S[tanisław] J.
Źródło: Neuropharmacology 2002: 43 (7) s.1082-1086, tab., bibliogr. [28] poz.
Sygnatura GBL: 305,141

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: (2S, 2R)-4-Methylglutamic acid (SYM 2081), a potent selective agonist of GluR5 and GluR6 kainate receptor subtypes, applied at the dose of 15.5 mg/kg, equal to its CD16 value (i.e., a dose required to induce convulsions in 16 p.c. of mice), significantly decreased the electroconvulsive threshold from 7.0 to 5.8 mA. When administered at the dose of 11.5 mg/kg, equal to 75 p.c. of its CD16, it markedly attenuated the protective activity of phenobarbital and diphenylhydantoin, but not that valproate, carbamazepine, or diazepam against maximal electroshock-induced seizures in mice. The respective ED50 values were increased from 18.5 to 23.8 mg/kg for phenobarbital, and from 11.7 to 14.7 mg/kg for diphenylhydantoin. Since the free plasma levels of tboth antiepileptic drugs were not influenced by SYM 2081, the pharmacokinetic interaction does not seem to be involved in the observed results. In conclusion, low-affinity kainate receptor-mediated events might be a factor reducing the protective efficacy of some anitepileptic drugs. Furthermore, the activation of GLuR5 and GluR6 kainate receptor subtypes by endogenous glutamate during seizures may be associated with the drug-resistance phenomenon.

1159/2523

Tytuł oryginału: Current concepts for peritoneal dialysis solutions.
Tytuł polski: Współczesne poglądy na płyny do dializy otrzewnowej.
Autorzy: Grzegorzewska Alicja E., Leander Magdalena
Źródło: Adv. Clin. Exp. Med. 2002: 11 (4) s.419-426, bibliogr. 56 poz., streszcz. - 8 Polsko-Niemiecko-Czeskie Sympozjum pt. Nowoczesne aspekty nefrologii i nadciśnienia Wrocław 23-25.05. 2002
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: W pracy przedstawiono zalety i wady stosowania niestandardowych płynów do dializy otrzewnowej: płynu zawierającego polimer glukozy (PD-PG), aminokwasy (PD-AA) oraz płynu zawierającego bufor wodorowęglanowy lub wodorowęglanowo-mleczanowy. PD-PG poprawia ultrafiltrację podczas długich wymian płynu dzięki procesowi osmozy koloidalnej. Inne korzyści stosowania PD-PG obejmują: wyższe wskaźniki adekwatności dializy, lepszy profil lipidowy surowicy, brak niekorzystnego wpływu na łaknienie i lepszą biozgodność, w porównaniu do standardowych płynów zawierających glukozę. Cechy PD-PG czynią go idealnym płynem do wczesnego rozpoczynania leczenia dializą otrzewnową, do stosowania podczas długich wymian płynu w ciągłej ambulatoryjnej lub ciągłej cyklicznej dializie otrzewnowej oraz podczas zapalenia otrzewnej. PD-AA został zaprojektowany w celu uzupełnienia przezotrzewnowych strat aminokwasów i białek, a tym samym do poprawy stanu odżywienia chorych. PD-AA nie zawiera glukozy, co jest korzystne u chorych na cukrzycę i/lub chorych otyłych. Objawy uboczne PD-AA obejmują zwiększone tworzenie się mocznika i kwasu moczowego, wzrost stężenia homocysteiny w osoczu, obniżenie stężenia wodorowęglanów w surowicy. Uważa się, że receptura PD-AA może być jeszcze ulepszona. Płyny do dializy otrzewnowej zawierające bufor wodorowęglanowy zaprojektowano w celu uniknięcia cytotoksyczności połączenia niskiego pH z mleczanami. Płyny te są wskazane u chorych cierpiących na bóle brzucha podczas wpuszczania płynu dializacyjnego, u dzieci z zespołem Leigha mających mocznicę, i ogólnie - aby poprawić jakość leczenia dializą otrzewnową...

1160/2523

Tytuł oryginału: Insulin-like growth factor-I receptor and its relationships to receptors for oestrogen, progesterone and epidermal growth factor in primary breast carcinoma in women.
Tytuł polski: Receptor insulinopodobnego czynnika wzrostu-I i jego współzależności z receptorami estrogenów, progesteronu i czynnika wzrostu nabłonków w pierwotnych rakach sutka kobiet.
Autorzy: Kluska Anna, Skasko Elżbieta, Paszko Zygmunt
Źródło: Adv. Clin. Exp. Med. 2002: 11 (4) s.429-435, tab., bibliogr. 19 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wprowadzenie. Receptory hormonów steroidowych są znanymi predykcyjnymi i prognostycznymi wskaźnikami przebiegu raka sutka u kobiet. Receptory czynników wzrostu: insulinopodobnego czynnika wzrostu-I (IGF-I-R) i czynnika wzrostu nabłonków (EGF-R) mogą być również ważnymi czynnikami prognostycznymi. Cel pracy. Celem naszych badań była ocena stężenia receptora IGF-I w pierwotnych rakach sutka u kobiet oraz określenie jego korelacji z receptorami estrogenów, progesteronu i czynnika wzrostu nabłonków. Materiał i metody. W 524 pierwotnych rakach sutka kobiet oznaczono stężenie receptora insulinopodobnego czynnika wzrostu-I (IGF-I-R) oraz określono jego współzależność z receptorami estrogenów (ER), progesteronu (PR) i czynnika wzrostu nabłonków (EGF-R). Stężenia receptorów czynników wzrostu i hormonów steroidowych oznaczano techniką radioligandową. Wyniki. W badanej grupie raków sutka średnie stężenie receptora IGF-I wynosi 17,6 ń 0,9 fmol/mg białka błon. Średnie stężenie IGF-I-R w rakach kobiet miesiączkujących i po menopauzie nie różnią się między sobą znamiennie. Między stężeniami receptorów IGF-I, estrogenów i progesteronu występują dodatnie statystycznie znamienne korelacje. Zależności te, charakteryzujące się wysoką znamiennością, występują w całej badanej grupie raków, zarówno u kobiet miesiączkujących, jak i po menopauzie. Zależności między IGF-I-R i EGF-R są znamienne jedynie w grupie raków ER- i PR-ujemnych (chiý = 3,9124; p = 0,0479; Q = (+) 0,42). Wnioski. Analiza stężeń receptora IGF-I w grupach raków utworzonych ze względu na współwystępowanie w nich receptorów estrogenów i progesteronu sugeruje, że estrtogeny wywierają znacznie większy wpływ na ekspresję IGF-I-R niż progesteron.

1161/2523

Tytuł oryginału: Evaluation of types of cervical anastomoses in oesophageal reconstructive surgery.
Tytuł polski: Ocena typów zespoleń szyjnych w chirurgii wytwórczej przełyku.
Autorzy: Strutyńska-Karpińska Marta, Ciesielska Agnieszka, Błaszczuk Jerzy, Wierzbicki Jarosław, Zielony Andrzej, Taboła Renata
Źródło: Adv. Clin. Exp. Med. 2002: 11 (4) s.445-450, il., bibliogr. 17 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wprowadzenie. Wśród wielu czynników mających wpływ na funkcję przełyku zastępczego, właściwa średnica zespolenia szyjnego wydaje się jednym z najważniejszych. Materiał i metody. Artykuł przedstawia wyniki oceny klinicznej 2 typów zespoleń szyjnych: "koniec do końca" i "końcowo-boczno-bocznego". Analizie poddano 25 chorych obojga płci: 15 mężczyzn i 10 kobiet w wieku 24-71 lat po wykonanych operacjach wytwórczych przełyku z różnych uszypułowanych odcinków jelit. Wskazaniami do operacji były: pooparzeniowe bliznowe zwężenia w 7 i rak piersiowego odcinka przełyku w 18 przypadkach. Analiza była oparta na ocenie klinicznej, badaniach radiologicznych oraz endoskopowych. Wyniki. Funkcję zastępczego przełyku oceniono jako bardzo dobrą u 14 chorych z zespoleniem szyjnym typu "końcowo-boczno-bocznym". Średnica tego typu zespoleń wynosiła 13-16 mm. U 10 chorych, z zespoleniem szyjnym "koniec do końca" średnica zespolenia wynosiła 8-10 mm. Chorzy ci mieli niewielkie trudności w połykaniu pokarmów stałych i funkcję tego typu zespoleń oceniono jako satysfakcjonującą. U 80 proc. z nich w okresie pooperacyjnym wystąpiła przetoka zespolenia szyjnego, w wyniku której doszło do zwężenia zespolenia. U 1 chorego z zespoleniem typu "koniec do końca' na skutek pooperacyjnej przetoki zespolenia szyjnego, powstało pełne zwężenie zespolenia szyjnego co całkowicie uniemożliwiło odżywianie się przez usta. Wnioski. W naszej opinii, opartej na prezentowanym materiale chorych, zespolenie typu ...

Streszczenie angielskie: Background. Among various factors having an influence on normal function of artificial oesophagus proper cervical anastomosis diameter seems to be one of essentials. Material and methods. The paper presents results of clinical evaluation of two types of cervical anastomoses: "end to end" and "end-side to side". We examined 25 patients of both sexes: 15 men and 10 women aged between 24 and 71 after oesophagoplaties created of various pedicle bowel segments. Indications for the operation were: postburning cicatrical stenosis in 7 cases and carcinoma of the thoracic part of oesophagus in 18 cases. Our analysis was based on clinical review, radiological and endoscopic examinations. Results. We evaluated function of the created oesophagus as very good in14 patients with "end-side to side" cervical or pharyngeal anastomoses. Diameter of these kinds of anastomoses measured between 13 and 16 mm in radiological examination. In 10 patients with "end to end" cervical anastomosis the diameter was estimated between 8 and 10 mm. These patients had little difficulties in swallowing solid food and we found the function of this kind of anastomosis satisfactory. Of them 80 p.c. developed leakage of cervical anastomosis after operation what, in our opinion, resulted in stenosis of the junction. One patient because of leakage in "end to end" anastomosis developed its complete stricture and it made oral nutrition impossible. Conclusions. In our opinion, based on presented patients, the ...

1162/2523

Tytuł oryginału: Breast tubular adenoma - case report.
Tytuł polski: Gruczolak cewkowy sutka - opis przypadku.
Autorzy: Osiecka Beata J., Bronowicz Andrzej, Ziółkowski Piotr, Rabczyński Jerzy
Źródło: Adv. Clin. Exp. Med. 2002: 11 (4) s.513-515, il., bibliogr. 10 poz., streszcz.
Sygnatura GBL: 313,451

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: W pracy przedstawiono opis przypadku gruczolaka cewkowego sutka. Nowotwór został rozpoznany u 83-letniej kobiety jako guz w lewym sutku i usunięty chirurgicznie. Gruczolak cewkowy sutka jest bardzo rzadkim nowtworem i stwarza trudności w przedoperacyjnej diagnostyce. Rozpoznanie jest oparte na badaniu histopatologicznym poprzedzanym oceną śródoperacyjną guza.

1163/2523

Tytuł oryginału: Tick-borne encephalitis in North-Eastern Poland in 1997 - 2001: a retrospective study.
Autorzy: Grygorczuk Sambor, Mierzyńska Dorota, Zdrodowska Agnieszka, Zajkowska Joanna, Pancewicz Sławomir, Kondrusik Maciej, Świerzbińska Renata, Pryszmont Justyna, Hermanowska-Szpakowicz Teresa
Źródło: Scand. J. Infect. Dis. 2002: 34 (12) s.904-909, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 305,538

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of this study was to characterize the epidemiology and clinical features of tick-borne encephalitis in North-Eastern Poland. Clinical and epidemiological data were analysed of patients hospitalized with the diagnosis in the Department of the Infectious Diseases and Neuroinfections of the Medical University in Białystok in 1997-2001. Tick-borne encephalitis was diagnosed in 152 patients: 51 (34 p.c.) presented with meningitis, 89 (59 p.c.) with meningoencephatitis and 12 (8 p.c.) with meningoencephalomyelitis. Headache (84 p.c.) and fevwer (81 p.c.) were the most common symptoms. Meningeal signs were present in 137 patients (90 p.c.). Most common neurological abnormalities were: Oppenheim and Babinski signs (74 patients, 49 p.c.), ataxia (37, 24 p.c.), impaired consciousness (37, 24 p.c.) and pareses (16, 10 p.c.). Of patients examined, 146 (96 p.c.) had raised pleiocytosis, frequently accompanied by high cerebrospinal fluid protein concentration (90 p.c.), raised erythrocyte sedimentation rate (65 p.c.), peripheral blood leucocytosis (26 p.c.) and increased aminotransferase activity (16 p.c.). There was only 1 forest worker among the patients. Tick-borne encephalitis remains common in North-Eastern Poland but, possibly because of effective vaccination, it has virtually disappeared among forest employees. The diagnosis appears difficult in some cases, as meningeal signs may not be present and laboratory findings may not be suggestive of a viral infection.

1164/2523

Tytuł oryginału: High levels of osteoprotegerin and soluble receptor activator of nuclear factor kappaB ligand in serum of rheumatoid arthritis patients and their normalization after anti-tumor necrosis factor ŕ treatment.
Autorzy: Ziółkowska Maria, Kurowska Mariola, Radzikowska Anna, Łuszczykiewicz Grażyna, Wiland Piotr, Dziewczopolski Wojciech, Filipowicz-Sosnowska Anna, Pazdur Jacek, Szechiński Jacek, Kowalczewski Jacek, Rell-Bakalarska Maria, Maśliński Włodzimierz
Źródło: Arthritis Rheum. 2002: 46 (7) s.1744-1753, il., tab., bibliogr. 32 poz.
Sygnatura GBL: 305,037

Hasła klasyfikacyjne GBL:

reumatologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Objective. To test the hypotheses that 1) proinflammatory cytokines affect osteoprotegerin (OPG) and soluble receptor activator of nuclear factor kappaB ligand (sRANKL) production and therefore the OPG and sRANKL levels differ in rheumatoid arthritis (RA) patients in comparison with healthy individuals; and 2) anti-tumor necrosis factor ŕ (anti-TNFŕ) therapy influences OPG and sRNAKL levels. Methods. Sera were obtained from healthy individuals or RA patients receiving the combination of infliximab and methotrexate. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were isolated from RA patients. Fibroblast-like synoviocytes (FLS) were isolated from synovial tissue obtained at total knee replacement in RA patients. Supernatants from cells stimulated with cytokines were collected after culture in vitro. Concentrations of OPG and sRANKL were determined by enzymelinked immunosorbent assays. Results. A strong positive correlation between OPG concentration and age was observed in healthy individuals but not in RA patients. The OPG and sRANKL levels were higher in RA patients than in healthy controls. Cultured FLS spontaneously secreted much higher amounts of OPG than PBMCs or SFMCs. Proinflammatory cytokines enhanced OPG production. Anti-TNFŕ treatment resulted in the normalization of serum OPG and sRANKL levels in RA patients without influencing the OPG:sRANKL ratio. Conclusion. Although higher serum levels of OPG and sRANKL are present in RA patients than in healthy individuals, the ratio of OPG:sRANKL is similar. There is an age-dependent increase of OPG but not sRANKL levels in healthy subjects. Anti-TNFŕ treatment results in the normalization of elevated levels of OPG and sRANKL in RA patients.

1165/2523

Tytuł oryginału: Tetrabromobenzene induced hepatotoxicity in rats following repeated exposure.
Tytuł polski: Ocena hepatotoksyczności tetrabromobenzenu u szczurów w warunkach ekspozycji powtarzanej.
Autorzy: Frydrych Barbara, Czerski Jakub, Szymańska Jadwiga A.
Źródło: Bromatol. Chem. Toksykol. 2002: 35 (3) s.231-239, il., tab., bibliogr. 17 poz., sum.
Sygnatura GBL: 305,377

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Polibromowane benzeny są znajdowane w środowisku i w tkankach ludzkich. Ich obecność w środowisku może być wynikiem używania heksabromobenzenu jako związku uniepalniającego oraz procesów debrominacji HBB. Celem niniejszego badania było zbadanie toksyczności podostrej jednego z metabolitów środowiskowych tetrabromobenzenu (TBB) oraz sprawdzenie jego wpływu na syntezę hemu. Zaobserwowano wzrost względnej masy wątroby oraz zmiany w poziomach sumy cytochromów. Nie zaobserwowano istotnych statystycznie zmian dla innych wskaźników toksyczności (MDA, GSH, ALAT, ç-GT). Zaobserwowano wzrost aktywności ALA-S. Nie stwierdzono natomiast silnego działania porfirogennego tego związku.

1166/2523

2002
Tytuł angielski: Role of PTEN mutation in pathogenesis of pheochromocytoma : doctorate thesis
Autorzy: Puc Janusz
Opracowanie edytorskie: Gaciong Zbigniew (supervisor).; Akademia Medyczna Katedra i Klinika Chorób Wewnętrznych i Nadciśnienia Tętniczego w Warszawie
Źródło: 2002, 99 k. : il., tab., bibliogr. k. 86-99, sum., maszyn.
Sygnatura GBL: 45/19945

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1167/2523

Tytuł oryginału: Healthy start in life : a comparison between health in Poland and Sweden with the first four WHO Health for All Targets as a starting point : doctorate thesis
Autorzy: Pellmer-Wramner Kristina
Opracowanie edytorskie: Drygas Wojciech (supervis.).; Medical University Department of Preventive and Social Medicine in Łódź
Źródło: 2002, [12], 182, [5] s., [34] s. tabl. : bibliogr. s. 162-182, sum., maszyn.
Sygnatura GBL: 45/19746

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1168/2523

Tytuł oryginału: RET proto-oncogene germline mutation in pheochromocytoma patients - incidence and clinical consequences.
Tytuł polski: Częstość występowania i znaczenie kliniczne mutacji protoonkogenu RET u chorych z guzem chromochłonnym.
Autorzy: Pęczkowska Mariola, Januszewicz Andrzej, Franke Gerlind, Hoegerle Stefan, Szmigielski Cezary, Łoń Izabella, Cieśla Magdalena, Janas Jadwiga, Januszewicz Magdalena, Cybulska Idalia, Wocial Bożenna, Łapiński Mariusz, Cieśla Witold, Szostek Mieczysław, Januszewicz Włodzimierz, Neumann Hartmut P. H.
Źródło: Nadciśn. Tętn. 2002: 6 (4) s.279-284, tab., bibliogr. 22 poz., streszcz.
Sygnatura GBL: 313,470

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Wstęp: Dotychczasowe badania wskazują, że częstość zespołu mnogiej gruczolakowatości (MEN 2), w skład którego wchodzi guz chromochłonny, jest większa niż dotychczas sądzono. Zespół ten dziedziczony jest w sposób autosomalny dominujący i wywołuje go mutacja protoonkogenu RET. Celem pracy jest ocena częstości występowania oraz kliniczne znaczenie mutacji protoonkogenu RET u chorych z guzem chromochłonnym. Materiał i metody: Badania genetyczne w kierunku mutacji protoonkogenu RET przeprowadzono u 106 chorcyh (średni wiek: 49 ń 14,1 roku 26M, 80K) z rozpoznanym i potwierdzonym histopatologicznie guzem chromochłonnym. Pacjenci ci byli uprzednio hospitalizowani i leczeni w Klinice Chorób Wewnętrznych i Nadciśnienia Tętniczego Akademii Med. w Warszawie w latach 1957 - 1998 oraz w Klinice Nadciśnienia Tętniczego Instytutu Kardiologii w Warszawie od roku 1980 do 2001. Oceniano również stężenie kalcytoniny (CT), zarówno w warunkach podstawowych, jak i po stymulacji pentagastryną, oraz stężenie parathormonu. Wyniki: Obecność mutacji protoonkogenu RET wykazano u 8 chorych (7,4 proc.) - w eksonie 11, w kodonie 634,TGC na CGC u 5 chorych, u pozostałych 3 odpowiednio - w eksonie 11, kodonie 634, TGC na GGC, w eksonie 11, kodonie 634, TGC na TGG oraz w eksonie 13, kodonie 791, TAT na CGC. Nadczynność komórek C potwierdzona testem pentagastrynowym stwierdzono u 5 nosicieli, u 2 chorych wynik testu był wątpliwy, jedynie u 1 chorego stężenie kalcytoniny było prawidłowe. Prawidłowe stężenie CT obserwowano u chorego z mutacją w eksonie ...

1169/2523

Tytuł oryginału: Georgia.
Autorzy: Gamkrelidze Amiran, Atun Rifat, Gotsadze George, MacLehose Laura
Opracowanie edytorskie: MacLehose Laura (ed.), McKee Martin (ed.).
Źródło: Healt Care Syst. Transit. 2002: 4 (2) 2002 s.I-VIII, 1-72, il., tab., bibliogr. 52 poz.
Sygnatura GBL: 554,698

Hasła klasyfikacyjne GBL:

ekonomika

Typ dokumentu:

1170/2523

Tytuł oryginału: Slovenia.
Autorzy: Albreht Tit, Cesen Marjan, Hindle Don, Jakubowski Elke, Kramberger Boris, Petric Vesna Kerstin, Premik Marjan, Toth Martin
Opracowanie edytorskie: Jakubowski Elke (ed.).
Źródło: Healt Care Syst. Transit. 2002: 4 (3) s.I-VIII], 1-85, [1-4], il., tab., bibliogr. [7] poz.
Sygnatura GBL: 554,698

Hasła klasyfikacyjne GBL:

ekonomika

Typ dokumentu:

1171/2523

Tytuł oryginału: World Health Organization : Regional Committee for Europe : report of the fifty-second session
World Health Organization Regional Committee for Europe
Źródło: - Copenhagen, WHO 2002, [4], 72 s., 30 cm. - Sesja Regionalnego Komitetu Europejskiego Światowej Organizacji Zdrowia Kopenhaga 16-19.09. 2002
Sygnatura GBL: 805,601

Hasła klasyfikacyjne GBL:

organizacja ochrony zdrowia

Typ dokumentu:

1172/2523

Tytuł oryginału: Assuring availability of opioid analgesics for palliative care : report on a WHO workshop
World Health Organization
Źródło: - Budapest, WHO 2002, [6], 139 s. : il., 30 cm. - Warsztaty pt. Zapewnienie dostępności środków przeciwbólowych opioidowych w opiece paliatywnej Budapeszt 25-27.02. 2002
Sygnatura GBL: 805,608

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

1173/2523

Tytuł oryginału: LDL-cholesterol and coronary artery disease: where we've been, where we're going.
Autorzy: Sumner Andrew D.
Źródło: Folia Cardiol. 2002: 9 (3) s.185-192, tab., bibliogr. 42 poz.
Sygnatura GBL: 313,196

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1174/2523

Tytuł oryginału: The polymorphism of the HERG gene responsible for the autosomal dominant long-QT syndrome.
Autorzy: Herbert Ernest, Trusz-Gluza Maria, Moric Ewa, Śmiłowska-Dzielicka Ewa, Mazurek Urszula, Wilczok Tadeusz
Źródło: Folia Cardiol. 2002: 9 (3) s.193-202, il., tab., bibliogr. 64 poz.
Sygnatura GBL: 313,196

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1175/2523

Tytuł oryginału: The Six Annual Meeting of the ESC Working Group on Echocardiography. In collaboration with the Working Group on Echocardiography of the German Society of Cardiology: [abstracts].
Źródło: Eur. J. Echocardiogr. 2002: 3 suppl. 1 s.[9], S1-S131, il., tab. - 6 Doroczne Spotkanie Grupy Roboczej Echokardiografii Europejskiego Towarzystwa Kardiologicznego we współpracy z Grupą Roboczą Echokardiografii Niemieckiego Towarzystwa Kardiologicznego Monachium 04-07.12. 2002
Sygnatura GBL: 313,590

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Temat korporatywny:

Europejskie Towarzystwo Kardiologiczne

1176/2523

Tytuł oryginału: IX Instructional Course Lectures of EFORT: [abstracts].
Autorzy: Czerwiński Edward
Źródło: J. Bone Joint Surg. Br. 2002: 84-B suppl. 3 s.349-378 - 9 Instruktażowy Kurs Wykładów EFORT [Europejska Federacja Towarzystw Ortopedycznych] Kraków 24-26.10. 2002
Sygnatura GBL: 310,919

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1177/2523

Tytuł oryginału: Reactive oxygen intermediates production and mannan binding lectin concentration in early pregnancy complicated by type 1 diabetes mellitus - preliminary report.
Tytuł polski: Ocena produkcji reaktywnych form tlenu i stężenia białka wiążącego mannozę we wczesnej ciąży powikłanej cukrzycą typu 1 - doniesienie wstępne.
Autorzy: Pertyńska-Marczewska Małgorzata, Tchórzewski Henryk, Cedzyński Maciej, Sobczak Małgorzata, Lewkowicz Przemysław, Cypryk Katarzyna, Podciechowski Leszek, Wilczyński Jan
Źródło: Diabetol. Pol. 2002: 9 (4) s.181-185, il., tab., bibliogr. 31 poz., sum. - 3 Kongres Polskiego Towarzystwa Medycyny Perinatalnej Łódź 25-27.09. 2002
Sygnatura GBL: 313,197

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie polskie: Układ dopełniacza odgrywa ważną rolę we wrodzonej i nabytej odpornosci. Może być on aktywowany na trzech drogach: klasycznej, alternatywnej i lektynowej. MBL (mannan-binding lectin) odgrywą ważną rolę w odporności wrodzonej. Wiele doniesień wskazuje na to, że u chorych na cukrzycę obserwuje się wzmożoną produkcję reaktywnych form tlenu (RFT) przez neutrofile krwi obwodowej. Wiadomo także, że składowe wrodzonej aktywności są aktywowane w czasie ciąży. Cel pracy. Ocena produkcji RFT oraz stęzenia MBL we wczesnej ciąży matek z cukrzycą oraz zdrowych ciężarnych. Materiał: W ocenie stężenia MBL badaną grupę stanowiło: 7 ciężarnych chorych na cukrzycę, z dobrym wyrównaniem metabolicznym między 7. a 13. tygodniem pierwszej ciąży oraz 10 zdrowych ciężarnych kobiet między 7. a 13. tygodniem pierwszej ciąży. Grupami kontrolnymi było 30 zdrowych, nieciężarnych kobiet oraz 9 nieciężarnych kobiet chorujących na cukrzycę typu 1. W celu oceny produkcji RFT przebadano 12 ciężarnych chorych na cukrzycę, pomiędzy 7-13 tygodniem ciąży. 14 zdrowych nieciężarnych, 14 chorych na cukrzycę nieciężarnych kobiet; oraz 14 zdrowych ciężarnych pomiędzy 7-13 tygodniem ciąży. W celu oceny stężenia MBL zastosowano zmodyfikowana mwetodę Aittonniemiego. Do analizy statystycznej użyto testu U Manna-Whitney'a. Produkcja ROI została oceniono metodą chemiluminescencji, przy użyciu stymulatorów fMLP, PNA, OZ oraz po 15 min. primingu TNF-ŕ. Zastosowano MLX Microtier Plate Luminometr DYNEX, USA. Wraz z programem komputerowym Revelation 97. Wyniki: 1. Zjawisko primingu...

1178/2523

Tytuł oryginału: The expressions of intracellular cytokines in the lymphocytes of preeclamptic patients.
Autorzy: Darmochwał-Kolarz Dorota, Roliński Jacek, Leszczyńska-Gorzelak Bożena, Oleszczuk Jan
Źródło: Am. J. Reprod. Immunol. 2002: 48 (6) s.381-386, il., tab., bibliogr. 45 poz.
Sygnatura GBL: 313,270

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Purpose: The objective of the study was to investigate the intracellular expressions of T helper 1 (Th1) and Th2 cytokines in peripheral blood T lymphocytes and natural killer (NK) cells of patients with preeclampsia and women with uncomplicated pregnancy. Method of study: Blood samples were taken from 20 patients with preeclampsia and 16 healthy pregnant women. Mononuclear cells were isolated from peripheral blood and stimulated for 5 hr at 37řC and 5 p.c. CO2. Next, the cells were stained with antibodies against surface markers of T-cell subsets and NK cells. After fixation and permeabilization processes, the cells were stained with antibodies against intracellular cytokines - interleukin-2 (Il-2) and interferon-ç (IFN-ç) as well as Il-10 and Il-4. The intracellular expressions of Th1 and Th2 cytokines were determined using the flow cytometric method. Statistical analysis was performed using Mann-Whitney U-test. Results: We found that in patients with preeclampsia the expressions of Il-2 were significantly higher when compared with women with uncomplicated pregnancy. Furthermore, in the group of patients with preeclampsia the expressions of Il-2 were higher in T CD 8+ lymphocytes than in T CD4+ cells. The expressions of IFN-ç did not differ in CD4+ cells and CD8+ cells in both studied groups but they were higher in NK cells in the study group. The expressions of Il-10 were lower in lymphocytes of preeclamptic patients when compared with controls. The expressions of Il-4 did not differ in both studied groups. Conclusions: These results suggest that in patients with preeclampsia there in Th1/Th2 imbalance, with predominant Th1 immunity.

1179/2523

Tytuł oryginału: Exercise decreases plasma total homocysteine in overweight young women with polycystic ovary syndrome.
Autorzy: Randeva Harpal S., Lewandowski Krzysztof C., Drzewoski Józef, Brooke-Wavell Katherine, O'Callaghan Chris, Czupryniak Leszek, Hillhouse Edward W., Prelevic Gordana M.
Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (10) s.4496-4501, il., tab., bibliogr. 57 poz.
Sygnatura GBL: 310,620

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Women with polycystic ovary syndrome (PCOS) have a clustering of cardiovascular risk factors, such as obesity, lipid abnormalities, impaired glucose tolerance, insulin resistance, and hypertension. Exercise is reported to lower the incidence of cardiac events. The effect of exercise on plasma homocysteine concentrations, an independent cardiovascular risk factor, has not been previously reported in women with PCOS. We examined the effects of exercise on plasma total homocysteine concentrations in young overweight or obese PCOS women [age (mean ń SD), 30.6 ń 6,6 yr; body mass index, 35.49 ń 7.57 kg/mý]. Twenty-one women consented to a 6-month exercise program; 12 women (exercisers) adhered to the program, whereas 9 (nonexercisers) did not. In both groups of women, the following parameters were recorded at baseline and 6 months: body mass index, waist-to-hip ratio, and aerobic capacity (maximal oxygen consumption); blood samples were taken after an overnight fast for plasma total homocysteine, insulin, and other biochemical parameters. A significant decrease in plasma total homocysteine concentrations (P 0.001) and waist-to-hip ratio (P = 0.041) and a significant increase in maximal oxygen consumption (P = 0.019) were recorded at 6 months, compared with baseline in the exercise group. This decrease in homocysteine was not explained by changes in anthropometric or biochemical parameters. In contrast, no significant changes in any of the variables were observed in the nonexercise group. Our study has provided the first evidence that regular exercise ignificantly lowers...

1180/2523

Tytuł oryginału: Plasma interleukin-8 concentrations are increased in obese subjects and related to fat mass and tumor necrosis factor-ŕ system.
Autorzy: Strączkowski Marek, Dzienis-Strączkowska Stella, Stępień Agnieszka, Kowalska Irina, Szelachowska Małgorzata, Kinalska Ida
Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (10) s.4602-4606, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 310,620

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Obesity is associated with the increased risk of cardiovascular disease; however, mechanisms responsible for such an increase are not fully understood. IL-8 is a cytokine that might have atherogenic properties. Recent in vitro studies revealed that IL-8 is produced and secreted by human adipocytes. The aim of the present study was to evaluate plasma IL-8 concentrations in obese subjects and the realationships between circulating IL-8 and anthropometric and biochemical parameters and TNF-ŕ system. A total of 75 subjects with normal glucose tolerance, 35 lean and 40 obese, were recruited for this study. Plasma IL-8 levels were measured in fasting state, after an oral glucose tolerance test and after the euglycemic hyperinsulinemic clamp. A significant increase in plasma IL-8 was observed in the obese group. In simple regression analysis, performed for the initial evaluation of relationships, plasma IL-8 was related to body mass index, percentage of boody fat, fat mass (FM), and soluble TNF-ŕ receptor 2(sTNFR2) in the obese. In multiple regression analysis, FM, waist-to-hip ratio, gender, sTNFR2, and low density lipoprotein cholesterol were responsible for 44 p.c. of IL-8 variability. During oral glucose tolerance testing, mean plasma Il-8 concentrations increased in booth groups, whereas clamp resulted in a significant increase in plasma Il-8 only in the obese. We conclude that plasma IL-8 levels are increased in obese subjects, and are related to FM and TNF-ŕ system. Increase in circulating IL-8 might be one of the factors linking obesity with greater cardiovascular risk.

1181/2523

Tytuł oryginału: Schistosoma haematobium: Cytochemistry of the Mehlis gland and of the ootype wall.
Autorzy: Moczoń Tadeusz, Świderski Zdzisław
Źródło: Acta Parasitol. 2002: 47 (4) s.280-287, il., bibliogr. [28] poz.
Sygnatura GBL: 303,018

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Secretory granules produced by the Mehlis' gland and by the wall of the distal ootype in Schistosomia haematobium females, were examined at the ultrastructural level by means of several cytochemical methods. Strong staining, due to a relatively high percentage of carbohydrates in the granules, was observed in polymethacrylate sections treated with a phosphotungstic acidhydrochloric acid mixture at pH = 0.5. Staining with ferric chloride-mixed-diamine mixtures (the LID and the HID reaction) showed no detectable level of oligosaccharide carboxyl and sulphate groups. A periodic acid-thiocarbohydrazide-silver proteinate reaction which was performed on epoxy sections, revealed a relatively high level of glycopyranosidic 1,2-diol groups in the granules. These results as well as the sensitivity of the secretory granules to the proteolytic action of papain indicated that the secretions produced by the Mehlis' gland and by the ootype wall were neutral glycoproteins.

1182/2523

Tytuł oryginału: Flow cytometric analysis of multiple myeloma cells in patients treated with high dose chemotherapy and hematopoietic stem cell transplantation : doctorate thesis
Autorzy: Abdulnaser Ali Awedan
Opracowanie edytorskie: Jędrzejczak Wiesław W. (superv.).; The Medical University Department of Hematology, Oncology and Internal Medicine in Warsaw
Źródło: 2002, III, 86 k. : il., tab., bibliogr. 109 poz., sum., maszyn.
Sygnatura GBL: 45/19975

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

1183/2523

Tytuł oryginału: Spondylo-epiphyseal dysplasia congenita Spranger-Wiedemann - a case report.
Tytuł polski: Wrodzona dysplazja kręgów i nasad kości Sprangera-Wiedemanna.
Autorzy: Beighton Peter, Kozłowski Kazimierz
Źródło: Pol. J. Radiol. 2002: 67 (4) s.81-84, il., bibliogr. 5 poz.
Sygnatura GBL: 301,279

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: A case of a 6-years old boy with spondylo-epiphyseal dysplasia congenital Spranger-Wiedemann type was assessed. The major symptom was bilateral coax vara. This is the first report of this disorder in the Polish literature. In children with bilateral coxa vara the differential diagnosis includes heritable bone dysplasias, notably spondylo-metaphyseal dysplasis Sutcliffe type (comer fracture type) and sponydlo-epiphyseal dysplasia congenita Spranger-Wiedemann type. Diagnostic precision is essential for the clinical and genetic management.

1184/2523

Tytuł oryginału: Ketogenic diet: does acetone stop seizures?
Autorzy: Likhodii Sergei S., Burnham W. McIntyre
Źródło: Med. Sci. Monitor 2002: 8 (8) s.HY19-HY24, il., tab., bibliogr. 40 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Background: The mechanism of action of the ketogenic diet, a therapy for refractory epilepsy, is unknown. Our hypothesis is that acetone, one of three ketones elevated by the ketogenic diet, is directly responsible for the diet's anticonvulsant effects. This study examined the basic concepts of this hypothesis. Material/Methods: Rats were acutely injected with acetone intraperitoneally at doses of 1 or 10 mmol/kg, or received acetone chronically in drinking water (1 p.c. v/v) for 10 days before being injected with 1 mmol/kg dose of acetone. Controls consumed regular water and were injected with vehicle. A pentylenetetrazole seizure test was administered 15 min after the injections. Following the test, acetone was measured in the cerebrospinal fluid. Results: A 10 mmol/kg injection of acetone suppressed seizures in 60 p.c. of rats (P 0.05). A chronic adiministration of acetone followed by a 1 mmol/kg injection suppressed seizures in 47 p.c. of rats (P 0.05). The acetone concentrations in these rats were 10.3 ń 2.3 and 1.0 ń 0.2 mmol/L, respectively. The effect of the acute 1 mmol/g injection (without acetone pretreatment) was not statistically significant. This dose elevated acetone to 1.1 ń 0.1 mmol/L in the cerebrospinal fluid. Conclusion: Our findings suggest that acetone is an anticonvulsant and that chronic administration may enhance its action. Linking acetone to the effects of the ketogenic diet requires further research. In particular, it will be important to confirm that the ketogenic diet generates relevang concentrations of acetone.

1185/2523

Tytuł oryginału: Elevated plasma gamma-aminobutyric acid (GABA) levels in autistic youngsters: stimulus for a GABA hypothesis of autism.
Autorzy: Dhossche Dirk, Applegate Heather, Abraham Ann, Maertens Paul, Bland Lorna, Bencsath Aladar, Martinez Jos
Źródło: Med. Sci. Monitor 2002: 8 (8) s.PR1-PR6, il., bibliogr. 59 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Autistic Disorder is an early-onset developmental disorder with severe lifelong impact on social functioning, communication, and behavior. There is currently no marker or cure. The pathophysiology and etiology are obscure. Evidence for abnormal gamma-aminobutyric acid (GABA) function in Autistic Disorders is limited. A few case-reports and small studies have reported differences in GABA levels in plasma, platelets, and urine, compared to controls. Further studies on abnormalities of GABA function in Autistic Disorder are warranted. Material/methods: Plasma GABA levels were measured using a new and sensitive technique, based on gas chromatography/mass spectrometry, in a small group of youngsters with Autistic Disorder and Attention-Deficit/Hyperactivity Disorder. Participants were outpatients between ages 5-15, satisfying modern criteria for these disorders. Results: Elevated plasma GABA levels were found in youngsters with Autistic Disorder. Psychotropic medications did not seem to affect plasma GABA levels in this study. Plasma GABA levels decreased with age. Conclusions: Elevated plasma GABA levels may be a biochemical marker of Autistic Disorder. This study supports the hypothesis that GABAergic mechansms play a role in the etiology or pathophysiology of Autistic Disorder. However, the hypothesis remains unspecified owing to lack of research. Future studies on the clinical associations of seizure disorders, mood disorders, and catatonia in autistic people may provide the necessary data to formulate a coherent theory of GABA dysfunction in Autistic Disorder. More trials of medication with known or suspected effects on GABA function are qarranted.

1186/2523

Tytuł oryginału: Recognition of substrate and Skp1 by the Homologue of Slimb (HOS) ubiquitin ligase receptor - role of the F-box.
Autorzy: Herter Jason R., Fuchs Serge Y.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR283-BR288, il., bibliogr. 28 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: SCFHOS-Rocl E3 ubiquitin ligase is an enzymatic complex, which mediates ubiquitination and subsequent proteasome-dependent degradation of phosphorylated inhibitor of NF-kappaB (IkappaB)and á-catenin. HOS is a WD40 repeats/F-box-containing protein that actually associates with the substrates and binds to Skpl1 via the F-box. Material/Methods: Here, we have studied the structural determinants of teh substrate recognition and ligase recruitment by HOS. The binding (pull-down and immunoprecipitation assays) and ubiquitination assays were perforemed in vitro with purified or partially purified recombinant proteins obtained via expression in bacteria or mammalian cells or by in vitro translation. Results: We identified specific amino acid residues (1143 and L152)) within the F-box of HOS that play a critical role in maintaining the hydrophobic interface of HOS-Skp1 interaction and found substantial similarity between interaction of Skp1 with HOS and with another F-box protein Skp2. Binding of Skp1 augments the ability of HOS to recognize the phosphorylated IkappaBŕ. Conclusions: These observations indicate teh role of teh F-box of HOS in both recruitment of ubiquitin ligase activity and substrate recognition as well as identify the structural elements that are important for both functions of HOS F-box domain. Annbreviations: HOS - Homologue of Slimb; IkappaB - inhibitor of NF-kappaB; á-TrCP - beta-transducin repeats-containing protein; SCF - Skp1-Cullin1-F-box protein complex, NMR - nuclear magnetic resonance.

1187/2523

Tytuł oryginału: Use of a non-contact radiant heat bandage on ischemic dermal infections in an ovine model.
Autorzy: d'Audiffrent Alexandre, Roethle Scott, Tretinyak Alexander, Santilli Steven
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR289-BR292, il., bibliogr. 14 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Background: Chronic non-healing foot wounds are common complication in the diabetic population. Local radiant heat bandage has recently been proposed as an effective adjuvant. The purpose of this study was to evaluate the efficacy of such bandage in controlling infection in an ovine ischemic wound model. Material/Methods: Bilateral flank ischemic wounds were created in a total of 42 sheep. 14 sheep were challenged with Pseudomonas aeruginosa (PA), 13 with Escherichia Coli (EC), and 15 with Methicillin resistant Staphylococcus aureus (MRSA). The left flank was designated the treatment side and the right the control side. The radiant heat bandage was applied for a total of 10 days. The animal were then euthanized and the wounds harvested for bacterial quantification. Results: 39 sheep completed the study. Mean bacterial counts were has follows: for MRSA, control 7.6 x 10**5 CFU/gm vs. heated 2.0 x 10**5 CFU/gm (p = 0.16); for EC, control 1.1 x 10**6 CFU/gm vs. heated 2.7 x 10**5 CFU/gm (p = 0.006); PA, control 1.7 x 10**6 CFU/gm vs. heated 3.9 x 10**9 CFU/gm (p = 0.001). Conclusion: Non-contact radiant bandages controls bacterial growth in ischemic wounds infected with MRSA or EC and may potentially improve wound healing. Wounds infected with PA should no be submitted to such treatment.

1188/2523

Tytuł oryginału: Differential expression of the mannose 6-phosphate/insulin-like growth factor-II receptor in human breast cancer cell lines of different invasive potential.
Autorzy: Xie Suqing, Kang Jing X.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR293-BR300, il., bibliogr. 28 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Hypersecretion of the precursor of the lysosomal protease cathepsin D (procatherpsin D) has been implicated in the invasive phenotype of human breast cancer. However, the mechanism of the abnormal secretion of procathepsin D remains unclear. Since the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) plays a central role in the intracellular transport and endocytosis of M6P-containing lysosomal enzymes, a deficiency in functional M6P/IGF2R may underline the hypersecretion of procathepsin D in invasive tumors. Materal/Methds: In the present study, we compared the profiles of cathepsin D and the M6P/IGF2R between the highly invasive MDA-MB-231 and the non-invasive MCF-7 cell lines. Results: MDA-MB-231 cells were confirmed to secrete a much larger proportion of procathepsin D into the medium than MCF-7 cells. Addition of M6P to the culture medium significantly altered the secretion of procathepsin D by MCF-7 cells, but had little effect on cathepsin D distribution in MDA-MB-23 cells. Both the M6P-binding capacity and the endocytosis of exogenous M6P-bearing proteins in MDA-MB-231 cells were far less than those in MCF-7 cells. mRNA analysis indicated that the levels of the M6P/IGF2R mRNA in MDA-MB-231 cells were not lower but were even higher than that in MCF-7 cells. Sequence analysis indicated a difference in the 3'-untranslated region of M6P/IGF2R between the two cell lines, but no mutation in the M6P-binding domain of the receptor. Conclusions: The results suggest that a potential defect in a post-transcriptional process (e.g., translation) may exist during synthesis of the m6P/IGF2R n MDA-MB-231 cells, leading to failure to express sufficient functional M6P/IGF2R and thereby resulting in the hypersecretion of procathepsin D...

1189/2523

Tytuł oryginału: Structural criteria of biologically active RGD-sites for analysis of protein cellular function - a bioinformatics study.
Autorzy: Torshin Ivan Y.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR301-BR312, il., tab., bibliogr. 73 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Background: Cell adhesion involves interactions of integrins and extracellular proteins, often facilitated by the RGD motif. Only presence of the RGD in a sequence of a protein may be not sufficient for the biological activity (binding to an integrin) and additional biochemical and/or structural studies are essential. Material/Methods: Structural criteria that would allow identification biologically active RGD-sites on the base of a spatial structure may assist analysis of function of a protein in the cell. For the first time, computational analysis of RGD-sites in a large non-redundant set of protein structures was done. Results: out of 3819 protein chains sequences of about 100 contained RGDs. Analysis of the structures of the RGD-'native' proteins has allowed establishing main determinants of the biologically active conformations of the RGD sites: surface accessibility of the whole RGD-sequence and the secondary structure. The criteria, applied to the reamining proteins of the set, identify 23 proteins (~ 25 p.c.) with potentially active RGD-sites. The results strongly suggest that RGD has a high propensity for being involved in protein-protein interactions and this may explain occurrence of RGDs in intracellular proteins. Results of the analysis suggest (in some cases, confirm) novel integrin-related activities for 7 membrane/extracellular proteins, as well as confirm RGD-facilitated cell attachment for 5 viral proteins. Conclusions: Only presence of RGD in a sequence is not sufficient to propose biological activity of this site. The results also suggest that the method can be used on large scale: for example, for identifying potential integrin-interacting proteins in an animal genome.

1190/2523

Tytuł oryginału: Activation of human polymorphonuclear cells induces formation of functional gap junctions and expression of connexins.
Autorzy: Branes Maria C., Contreras Jorge E., Saez Juan C.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR313-BR323, il., tab., bibliogr. 58 poz.
Sygnatura GBL: 313,278

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Background: During inflammatory responses activated polymorphonuclear cells (PMNs) adhere to each other and form clusters within the vasculature or injured tissues. We hypothesized that conditions that partially mimic the chemical environment of inflammatory foci induce the expression of functional gap junctions (GJs) between cultures PMNs. Material/Methods: Human PMNs were treated with bacterial lipopolysaccharide (LPS), TNF-ŕ, LPS plus medium conditioned by LPS-treated endothelial cells (ECs) or TNF-ŕ plus ECs conditioned medium. Gap junctional communication was evaluated with the dye coupling technique using a permeant and an impermeant GJ fluorescent dye and GJ lockers. The expression of connexins, GJ protein subunits, was evaluated by immunocytochemistry and immunoblotting. Cytochalasin-D and nocodazole were used to evalaute the involvement of cytoskeleton in the induction of dye coupling. Results: Treatment with LPS or TNF-ŕ induced the formation of PMN aggregates, but cells were not dye coupled. If the latter protocols occurred in medium conditioned by LPS-treated ECs or resting ECs, respectively, intercellular transfere only of the GJ permeant molecule was observed in most clustered cells. Dye coupling was reversibly inhibited by GJ blockers and prevented by cytochalasin-D, a microfilament disrupter, but not by noncodazole, a microtubule disrupter. Treatments that induced dye coupling also induced connexin43 and connexin40, but not connexin32 immunoreactivity. None of these connexins was detected in circulating cells. Conclusion: EC-derived factor(s) and micorofilament integrity are required for dye coupling between LPS- ane TNF-ŕ-treaated PMNs. GJ formation between PMNs is correlated with the presence of connexins43 and 40, but not 32 and requires intact microfilaments.

1191/2523

Tytuł oryginału: Levels of F1+2 prothrombin fragments and thrombin - antithrombin III (TAT) complexes in patients with dermatitis herpetiformis.
Autorzy: Wankiewicz Anna, Iwan-Ziętek Iza, Gwieździński Zenon, Kotschy Maria
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR324-BR327, tab., bibliogr. 14 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Dermatitis herpetiformis (DH) is a rare bullous disease of autoimmune etiology. It is an intestinocutaneous syndrome, in which vesicopapular cutaneous lesions are accompanied by gluten-dependent enteropathy. The diagnosis of DH is based on immunopathological investigation of unaffected skin fragment (granular IgA deposits present at the tops of dermal papillae and IgA - EMA present in the serum). Material/Methods: The studied group consisted of 33 patients with dermatitis herpetiformis - 23 males and 10 females aged 22-78 (mean age 44.7). In all the patients, the following parameters of blood coagulation systems were determined in the plasma levels of prothrombin fragments 1+2 (F1+2), levels of thrombin - antithrombin III (TAT) complexes, antithrombin III activity (AT III). Results: In patients with dermatitis herpetiformis, the main level of F1+2 fragments amounted to 9.08 nmol/l, as compared with 1.14 nmol/l in the control group (the difference statistically significant, p 0.0001). In DH patients, the levels of TAT complexes were over twice higher than among the controls and that difference also reached statistical significance (p 0.0001). Mean antithrombin III activity (AT III) in patients with DH reached 102.60 p.c. and was similar to that observed in the control group (104.68 p.c.) Conclusions: In patients with dermatitis herpetiformis, increased levels of F1+2 prothrombin fragments and TAT complexes indicate enhanced in vivo thrombinogenesis. No statistically significant differences of F1+2 TAT and AT III levels in DH patients related to their sex, age and duration of the disease were observed.

1192/2523

Tytuł oryginału: Neuroprotective effect of garlic compounds in amyloid-á peptide-induced apoptosis in vitro.
Autorzy: Peng Qiaoling, Buz'Zard Amber R., Lau Benjamin H. S.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR328-BR337, il., bibliogr. 60 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Neuronal apoptosis is one of the pathoglogical features of Alzheimer's disease (AD) and is associated with senile plaques containing amyloid-á peptide (Aá). Reactive oxygen species (ROS)are proposed to be involved in the apoptotic mechanism of Aá-mediated neurotoxicity. The purpose of this study was to determine the effects of aged garlic extract (AGE) and S-allyl cysteine (SAC) on Aá25-35-induced apoptosis and ROS generation in a rat pheochromocytoma (PC12) cell line. Material/Methods: PC12 cells were grown in RPMI 1640 medium containing 5 p.c. fetal calf serum and 10 p.c. horse serum. Cells were incubated wtih AGE or SAC for 24 h prior to exposure to Aá25-35 for various times. Cell viability, DNA fragmentation, number of apoptotic cells, caspase activity and generation of ROS were determined. Results: Aá25-35-induced apoptosis in PC12 cells was demonstrated by: 1) A dose-dependent loss of cell viability; 2) A time- and dose-dependent increase in apoptotic cells; 3) An induction of DNA fragmentation; and 4) An increase in caspase-3 activity and cleavage of poly (ADP-ribose) polymerase (PARP). After exposing PC12 cells to Aá25-35, a significant increase in ROS preeceded apoptotic events. AGE and SAC not only suppressed the generation of ROS but also attenuated caspase-3 activation, DNA fragmentation, PARP cleavage and eventually protected against Aá-induced apoptosis. Conclusions: Our data suggest that ROS may be involved in Aá-induced apoptosis in PC12 cells. They further suggest that garlic compounds can reduce apoptosis, possibly by enhancing the endogeous antioxidant defenses.

1193/2523

Tytuł oryginału: Activity of superoxide dismutase and catalase and the level of lipid peroxidation products reactive with TBA in patients with psoriasis.
Autorzy: Drewa Gerard, Krzyżyńska-Malinowska Ewa, Woźniak Alina, Protas-Drozd Franciszka, Mila-Kierzenkowska Celestyna, Rozwodowska Małgorzata, Kowaliszyn Bogna, Czajkowski Rafał
Źródło: Med. Sci. Monitor 2002: 8 (8) s.BR338-BR343, tab., bibliogr. 34 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The etiology of psoriasis, one of the most common chronic dermatoses, has not been elucidated yet. Involvement of antioxidant enzymes is suspected. The study aimed to determine the concetrations of lipid pereoxidation products reactive with thiobarbituric acid and antioxidant enzymes activity. Material/Methods: 67 patients of the Chair and Clinic of Dermatology (22 females and 45 males) with psoriasis vulgaris of 3 to 34 years' duration were examined. All were treated topically with preparations for external use. Smooth skin was treated with salicyl ointment, cignoline and tar, while salicyl oil and betamethasone diproproionide with salicylic acid was used on the scalp. Lipid peroxidation products reactive with TBA, expressed as malondialdehyde (MDA) concentration, were determined in erythrocytes using Buege and Aust method. Activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were determined in erythrocytes according to Misra and Fridovich, and Beers adn Sizer, respectively. Results: In halthy subjects, SOD and CAT activity was 1049.2 ń 261.9 U/gHb and 41.4 ń 13.7 x 10**4 IU/gHb, respectively, and MDA concentration 35.8 ń 10.5 nM/gHb. MDA concentrations in erythrocytes of psoriatic patients before treatment were 44 p.c. higher and SOD and CAT activities 20 p.c. and 27 p.c. lower, respectively, than those observed in the controls. Topical treatment improved the patients' clinical condition, resulting in slow increase of enzyme activity to values comparable with controls. Conclusions: The results confirm the involvement of antioxidant enzymes in the pathogenesis of psoriasis. It is, however, questionable whether the observed abnormalities are responsible for the onset of psoriasis, or resultant from ongoing pathologic process.

1194/2523

Tytuł oryginału: Patient, community and clinician perceptions of the quality of life associated with diabetes mellitus.
Autorzy: Landy Jennifer, Stein Joshua D., Brown Melissa M., Brown Gary C., Sharma Sanjay
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR543-CR548, tab., bibliogr. 27 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: A study was undertaken to assess the quality of life of patients with type 1 and type 2 diabetes mellitus and to ascertain whether clinicians and non-diabetic respondents from the general public have similar views of the impact of diabetes upon health-related quality of life. Material/Methods: Time trade off utility values were generated from a standardized time-tradeoff questionnaire. Three hundred and fifty-two individuals with type 1 or type 2 diabetes mellitus, 157 non-diabetic participants from the general public (community), and 61 healt care clinicians participated in the study. Results: The mean utility score for diabetic patients was 0.889 and the median utility score was 1.000. The mean utility score for clinicians was 0.861, with a median value of 0.894, while the respective mean and median scores for the general public were 0.919 and 0.953. There was a significant difference between the disribution of the means of socres of clinicians and patients as well as between clinicians and the general public. There was no significant difference between the utility scores of patients and the general public. Within the group of diabetic patients, there was no significant difference in utility scores between type 1 and type 2 diabetics. Conclusions: Clinicians tended to over emphasize the impact that diabetes mellitus has on health related quality of life, while the non-diabetic publics' utility values are more closely correlated with those of diabetics themselves. We conclude that there is a significant difference in how clinicians, diabetics and the general public perceive the effect diabetes has upon quality of life.

1195/2523

Tytuł oryginału: Development of a site sampling form for evaluation of ED overcrowding.
Autorzy: Weiss Steven J., Arndahl Jeanine, Ernst Amy A., Derlet Robert, Richards John, Nick Todd G.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR549-CR553, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

organizacja ochrony zdrowia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: ED overcrowding is fast becoming a national crisis although no definition exists. The purpose of this study is to develop and pilot a sampling form that accurately reflects the concept of ED Overcrowding. Material/Methods: A 26-question site-sampling form was designed based on input from academic physicians at 11 medical schools nationwide. The study was conducted at an inner city Academic medical center. Site-samplings were conducted at 20 times over a one-week period by an independent observer. These times ranged from very slow to severely overcrowded. Information was obtained by counting patients in the waiting room, ED rooms, ED halls and registration/triage, ancillary services, the charge nurse, and the attending physicians. The charge nurse, and ED physicians rated the degree of overcrowding and the ED physician rated the feeling of being rushed. A 'combined outcome variable'was created which consisted of the average responses of nurses and physician's opinion of ED overcrowding and physician's feeling of being rushed. All other data was compared to this outcome variable. Results: Sever questions were significantly correlated with the combined outcoe variable. Tese were the number of people in the waiting room, patients awaiting triage, patients awaiting registration, full patient rooms, hallway patient, patients awaiting beds, and total registred patients. Accoridng to this scale our ED was overcrowded 20 p.c. of the time. Conclusions: This analysis clarifies the definition of overcrowding, helps indicate the variance among responses to overcrowding questions, and provides the foundation for prospective analysis of overcrowding in multiple EDs.

1196/2523

Tytuł oryginału: Incidence of complications of peptic ulcers in patients with Helicobacter pylori (Hp) infection and/or NSAID use in the era of Hp eradication.
Autorzy: Bobrzyński Andrzej, Bęben Piotr, Budzyński Andrzej, Bielański Władysław, Płonka Małgorzata, Konturek Stanisław J.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR554-CR557, il., bibliogr. 16 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Hp and NSAID are considered as major pathogens in peptic ulcerations and their complications but little is konwn about the incidence of ulcaers and their complications following widespread use of Hp eradication. The aim of the study were: 1) to analyze incidence of ulcers and their complications, bleeding and perforations at time when the HP eradicaton has been used in ulcer therapy, and 2) to assess the impact of Hp infection and NSAID use on the incidence of ulcers and complications. Material/Methods: From 1996 to 2001, 381 patients with complications of peptic ulcers were admitted to the emergency surgery, including 273 patients with bleeding ulcers and 108 with perforations out of a sample of 6515 dyspeptic patients examined with upper endoscopy and 13C-urea breath test (UBT). Results: The rate of ulcer bleeding and perforations, remained relatively constant throughout the study period. NSAID use in that group increased form 15.8 p.c. in 1999 to 19.3 p.c. in 2001. The incidence of HP in patients with complications assessd by UBT or CLO was 76.7 p.c., while the incidence of Hp in 7920 patients ranged form 72.8 p.c. in 1996 to 53.8 p.c. in 2001. There were 1940 (29.7 p.c.) patients with duodenal and/or gastric ulcer diagnosed by upper gastroscopy. The decline in the prevalence of peptic ulcer from about 44 p.c. to 8 p.c. occurred over the same time. A slight increase in the number of ulcer resulting from NSAID use was observed so was the number of ulcers without Hp or NSAID (idiopathic). Conclusions: Despite decreased Hp prevalence, the incidence of ulcers complications remained unchanged probably due to increased use of NSAID and the appearance of idiopathic ulcers.

1197/2523

Tytuł oryginału: Meta-analysis of 20 case-control studies on the N-acetyltransferase 2 acetylation status and colorectal cancer risk.
Autorzy: Ye Zheng, Parry James M.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR558-CR565, il., tab., bibliogr. 47 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Rapid NAT2 acetylation has been considered as a risk factor for developing colon cancer in a number of studies, however the overall results of such studies are inconsistent. To clarify the influence of NAT2 rapid acetylation status on colon cancer risk, we have performed a metaanalysis of 20 published case-control studies (4431 cases, 4547 controls). Material/Methods: Odd ration was employed to evaluate the risk of colon cancer and NAT2 rapid acetylation status. To take into account the possibility of heterogeneity across the studies a statistical test for heterogeneity across the studies was performed. The summary odds ratios were assessed by calculating a weighted average of odds ratios for all of the studies. Results: The pooling of studies based on phenotyping methods indicated that the overall odds ratio of colon cancer risk associated wtih rapid acetylator was 1.51 (95 p.c. Cl: 1.07 - 2.12). However, the risk of colon cancer associated with rapid acetylator from the studies based on genotyping method was lower with a calculated overall odds ratio of 1.06 (95 p.c. Cl: 0.97 - 1.15). Pooling studies were also conducted on specific tumour sites and ethnic groups. The results show that effect of rapid acetylator on colon cancer risk was not obviously different. Conslusions: The results of our meta-analyses do not support the hypothesis that NAT2 alone is an important risk factor for colon cancer and suggests that NAT2 rapid acetylation status has no specific effect on the risk of developing colon cancer.

1198/2523

Tytuł oryginału: Angiotensin I-converting enzyme insertion/deletion polymorphism and adrenergic response to exercise in hypertensive patients.
Autorzy: Jalil Jorge E., Córdova Samuel, Ocaranza Maria Paz, Schumacher Erwin, Braun Sandra, Chamorro Gastón, Fardella Carlos, Lavandero Sergio
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR566-CR571, il., tab., bibliogr. 39 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: The inserton/deletion ACE polymorphism (ACE I/D) regulates different levels of circulating and tissue ACE activities, which may induce divrse adrenergic responses to physiological stimuli. The aim of this study was to evaluate the influence of the ACE I/D polymorphism on the adrenergic response to isotonic exercise in middle-aged hypertensive patients. Material/Methods: Submaximal exercise (on a treadmill, using the Naughton protocol at 75 p.c. of maximal heart rate) was performed in 34 patients homozygous for the ACE I/D polymorphism (ACE II and ACE DD) with untreated essential hypertension (II = 19, DD = 15). Plasma venous adrenaline and noradrenaline were measured at rest and at submaximal exercise. Results: Plasma ACE activity was significantly higher inthe hypertensive patients carrying the ACE DD genotype compared with the ACE II group. Left atrium size, as well as LV dimensions, mass, and function, were similar in both gorups. Total exercise time, baseline and 75 p.c. maxmal heart rate (MHR) and blood pressure were similar in both groups. Baseline plasma adrenaline and noradrenaline levels were similar in both gorups and increased significantly (p 0.05) by ca 300 p.c. at submaximal exercise without differences between groups. Conclusions: The presence of the D allele on the ACE gene in middle-aged hypertensive pateints determines higher circulating ACE activity but not increased sympathetic activity in response to submaximal exercise.

1199/2523

Tytuł oryginału: Epidemiological surveillance of pediatric Hodgkin's disease in southern Iran.
Autorzy: Karimi Mehran, Yarmohammadi Hooman, Ghavanini Ahmad A., Kumar Perikala V.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR572-CR575, tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: To study the epidemiological pattern of Hodgkin's disease (HD) in children of Southern Iran, a total of 120 cases of biopsy-proven Hodgkin's disease, less than 18 years old, submitted for histological diagnosis during 1987 - 1997 were classified, using the Rye's Classification. Material/Methods: Data on age, sex, number in households, socio-economic status, clinical presentation, signs and symptoms, para-clinical data at the time of diagnosis, treatment plan, recurrence and the presence of metastases were derived from the patients' history, and analyzed. Results: The largest subgroups were those of Mixed celularity (66.7 p.c.), followed by Nodular sclerosis (23.3 p.c.), Lypmpocytic predominance (8.3 p.c.) and Lymphocytic depletion (1.7 p.c.). The male-to-remale ratio was 2.87. Patients with Nodular sclerosis were generally older than those in other groups. The mean age in all histopathological classes was higher in females. Conclusion: The epidemiological pattern of the HD in southern Iran is similar to that of other developing countries and the northern part of Iran, in which mixed callularity is the most common subtype. However, this pattern differs from the pattern seen in the United State and Europe, since Nodular sclerosis is the most common subtype in these regions.

1200/2523

Tytuł oryginału: Role of semantic control of action in the comprehensive rehabilitation of patients recovering from prolonged post-traumatic coma.
Autorzy: Pąchalska Maria, Talar Jan, MacQueen Bruce Duncan
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR576-CR586, il., tab., bibliogr. 44 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Apraxia, broadly understood as the acquired inability to perform learned, skilled movements not caused by paresis or incomprehension, occurs with greater frequency among patients' recovering from prolonged post-trauamtic coma than has previously been reported. The present article prevents an evaluation of the effectiveness of a rehabilitation program called Semantic Priming, aimed at rebuilding praxis in the patients. Material/Methods: Out of a consective series of 51 patients with post-traumatic coma lasting longer than 4 weeks treated at the centers represented by the authors, 24 were found to have pathological scores on the Boston Test of Praxis. These patients were divided into two matched groups, of wom the experimental group received the Semantic Priming program (involving the systematic use of verbal cues to prime movement activation patterns), and the control group did not. Progress was measured by the Boston Test of Praxis and a test developed by the authors on the basis of Luria's procedure for testing praxis. Results: After 5 weeks of therapy the patients in the experimental gorup showed significantly greater improvement in all measured parameters (p 0.001). Conclusions: Apraxia in the broader sense is a more common problem among post-coma patients than heretofore recognized, and causes concrete problems in rehabilitation and adjestment to daily living. Our program based on the use of semantic priming of activation proved to be highly succesful in remediating apraxia in these patients.

1201/2523

Tytuł oryginału: Incidence of childhood cancers in Poland in 1995-1999.
Autorzy: Kowalczyk Jerzy R., Dudkiewicz Ewa, Balwierz Walentyna, Bogusławska-Jaworska Janina, Rokicka-Milewska Roma
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR587-CR590, tab., bibliogr. 19 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: The annual rate for childhood cancers in developed countries amonunts to 105 - 130 new cases per 1 milion children. The Polish population aged 0 - 17 years is estimatd at approximately 10 milion children and adolescents, thus ca. 1100 - 1300 new cases can be expected every years. In 1995, we started a national childhood cancer registry. Material/Methods: information on the new diagnoses of childhood cancers was collected in 11 regional centers and submitted to the national center in Lublin. All data were verified carefully and standardized incidence rates were calculated. Results: In 1995, we registserd 1028 newly diagnosed malignant neoplasms, in 1996 and 1997 - 1036 cases, in 1998 - 1007, and in 1999 - 1158 new cases. The estimated incidence rates were: 102.3; 109.5; 111.9; 111.6 and 118.3 per 1 milion children, respectively. The most frequent childhood cancers include feukemia, which accounts for 28 p.c. of cancer cases, lymphoma (14.3 p.c.) and C. N. S. tumors (16.3 p.c.). Conclusions: Neoplasms of the hematopoietic system (leukemias and lymphomas) account for about 42 p.c. of all childhood cancers. Malignant lymphomas, bone tumors and germinal tumors are more frequently diagnosed in Poland, but the incidence of central nervous system tumors is lower than in other countries.

1202/2523

Tytuł oryginału: Clinical and epidemiological assessment of hepatitis C virus infection among voluntary blood donors.
Autorzy: Łabędzka Hanna, Simon Krzysztof, Gładysz Andrzej
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR591-CR596, tab., bibliogr. 30 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: This study analyzes the clinical and epidemiological characteristics of patients infected with hepatitis C virus (HCV), with emphasis on differences between voluntary multiple blood donors, first time blood donors, and non-donating patients with chronic or acute hepatitis C. Material/Methods: Our epidemiological and clinical assessment covered 119 voluntary blood donors, as well as 92 HCV patients and were seen in the same hosptal. Results: A variety of potential HCV transmission routes variety was demonstrated in both examined groups. The main infection routes were associated with diagnostic and therapeutic procedures performed in medical service units. Substantial time divergence was found between probable HCV infection and onset of first symptoms suggesting liver pathology or HCV antibody detection. No subjects from either groups showed specific symptoms pointing univocally at liver disease. Hepatomegaly was the most common symptom observed on physicial examination. No pathology of other organs which could possibly be associated with HCV infection was found in any patient. Increased aminotransferase activity was found in over 40 p.c of the donors, and over 90 p.c. of the non-donating patients on first examination. Liver biopsy histological assessment delivered the most reliable information concerning the degeree of liver pathology degree, which was slight in most cases. Conclusions: Multiple voluntary blood donation does not influence the clinical picture and sourse of HVC infection, not does it not increase the risk of HCV infection.

1203/2523

Tytuł oryginału: G-protein á3 subunit gene C825T polymorphism is associated with arterial hypertension in Polish patients with type 2 diabetes mellitus.
Autorzy: Dzida Grzegorz, Golon-Siekierska Patrycja, Puźniak Andrzej, Sobstyl Jacek, Biłan Andrzej, Mosiewicz Jerzy, Hanzlik Janusz
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR597-CR602, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Background: Diabetes mellitus type 2 results from a complex of hreditary and environmental factors. Genes encoding elements of the G-protein system are candidate genes in hypertension and obesity. Since insulin uses G-sensitive mechanisms to enhance tissue glucose uptake and vasodilatation, the GNB3 gene may be a candidate gene in type 2 diabetes. The goal of our research was to determine if the C825T polymorphism at the G-protein á3 subunit gene locus is associated wtih type 2 diabetes. Material/Methods: The study population consisted of 172 Polish patients with type 2 diabetes and 172 healthy, age- and sex-matched controls. The C825T polymorphism was detected by PCR and RFLP. Results: A higher frequency of genotypes containing the mutation (CT + TT) was observed among the diabetics than in the controls. The T825 variant occurred more often among hypertensive daibeatics (71 p.c.) than among diabetics with normal blood pressure (42.5 p.c.). The OR for hypertension in diabetic subjects bearing CT + TT genotypes was higher than in patients with the CC genotype. Overweight and obesity were not associated with teh T825 variant in either the experimental subjects or the controls. Conclusions: In this population, the T825 variant of the GNB3 gene was not associated withtype 2 diabetes itself, nor with overweight and obesity, but was associated with diabaetic hypertension. Upon confirmation of our results this variant may be useful as a genetic marker of susceptibility to hypertension and vascular complications in type 2 diabetes.

1204/2523

Tytuł oryginału: Angiotensin I-converting enzyme and chymase gene polymorphisms - relationship to left ventricular mass in type 2 diabetes patients.
Autorzy: Gumprecht Janusz, Zychma Marcin, Grzeszczak Władysław, Łącka Beata, Burak Wacław, Mosur Mariusz, Kaczmarski Jacek, Otulski Ireneusz, Stokłosa Tomasz, Czank Piotr
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR603-CR606, tab., bibliogr. 31 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Background: Type 2 diabetic patients are at increased risk for cardiovascular morbidity and mortality. Increased left ventricular mass also predicts a higher incidence of cardiovascular events. Angiotensin II is a potent mediator of myocardial growth, and angiotensin II can be produced in the heart by angiotensin I-convertig enzyme (ACE) and heart chymase (CMA). The aim of this study was to establish the role of ACE gene insertion/deletion (I/D) and CMA gene CMA/B polymorphisms in determining left ventricular mass in type 2 dibetic patients. Material/Methods: Echocardiographic measurements, ACE gene I/D and CMA/B genotypes were determined in 154 type 2 daibetic patients. Results: Mean LVMI was higher among DD homozygotes compared to heterozygotes and II homozygotes (128.9 g/mý vs. 120.5 g/mý and 120.4 g/mý, respectively), but the difference was not statistically significant (ANOVA P = 0.12). A similar effect was observed for the CMA/B polymorphism, where mean LVMI were 126.6 g/mý, 122.1 g/mý adn 118.2 g/mý, for carriers of AA, AG and GG genotype, respectively (not statistically significant, P = 0.33). ACE I/D adn CMA/B polymorphism were also analyzed jointly, and carriers of both DD and AA genotypes were found to have significantly higher LVMI values (P = 0.05) than non-carriers (133.0 g/mý and 121.2 g/mý, for 21 DD and AA carriers vs. 133 non-carriers). In multivariate analysis, the presence of DD and AA genotpyes was independently associated with LVMI (P = 0.04). Conclusion: Our results may suggest the additive effect of ACE and CMA gene polymorphisms on the increase in left ventricualr mass in NIDDM patients.

1205/2523

Tytuł oryginału: T-helper (1) cytokines increase during early pregnancy in women with a history of recurrent spontaneous abortion.
Autorzy: Rezaei Abbas, Dabbagh Ali
Źródło: Med. Sci. Monitor 2002: 8 (8) s.CR607-CR610, tab., bibliogr. 17 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ciąża

Streszczenie angielskie: Background: The measurement of various cytokines may provide different tools for evaluation of the immune system. The objective of this study was to access the role of the Th1 cytokines (IL-2, IFN-ç, TNF-ŕ, TNF-á) in this phenomenon, and detection of Th1 cytokines in women with RSA. Material/Methods: The control group consisted of 40 women with no history of pregnancy loss. The abortion group comprised of 92 women having at least 3 pregnancy losses. Blood samples from the control group and women with RSA were taken at the end of first trimester of gestational age and at the time of abortion, respectively. Sera were separated and lymphocytes were chalenged with PHA. All samples were tested for concnetration of Th1 cytokines using ELISA methods. Results: It was found that sera from women with RSA produce higher concentration of TNF-ŕ, TNF-á, and IL-2 in comparison with sera from normal pregnant women (NPW) (P 0.001). We were not able to detect IL-2 in (NPW) whereas in 31 p.c. sera from RSA group. IL-2 was detectable (P 0.001). However, there was no significant differences between IFN-ç, concentration in sera from patients and control group (P 0.182). Tissue culture supernatant from women with RSA also produced higher concentration of RNF-ŕ, IFN-çm TBF-á and IL2, than control group. Conclusions: These data may explain the increase NK-cells cytolytic activity during early pregnancy in RSA group. It may also provide a diagnostic tool to predict the outcome of pregnancy.

1206/2523

Tytuł oryginału: Fractal characteristics of brain vessel microangioarchitecture during the fetal period.
Autorzy: Rybaczuk Marek, Kędzia Alicja, Paradowski Leszek
Źródło: Med. Sci. Monitor 2002: 8 (8) s.MT145-MT152, il., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

noworodki

Streszczenie angielskie: Background: Studies of human telencephalon during the fetal period are rare. Recent technologies, such as the Pickworth method, ultraviolet, infrared, and computer image systems, now enable the observation of such barely accessible structures. The present study constitutes an evaluation of the microangioarchitecture of brain vessels during the fetal period and the creation of a fractal model to describe it. Studies formulated in the language of fractals have never previously been applied in modeling brain vessels. Material/Methods: We studied 100 brains in the 4th to 86h month of fetal life, using the Pickworth method, computer image analysis under three different systems, the POV-RAY program, and fractal analysis. Results: The POV-RAY program was used to generate 3-dimensional, tree-like structures. This requires three scaling parameters: length, vessel diameter, and the angle between the branches and the central axis. The vessel structure was modeled by fractals with condensation point. Moreover, these tree-like fractals have different one-sided dimensions. In making fractal models we treated these one-sided dimensions as numerically evaluted parameters of the theory. Of course, the accuracy of the proposed models depends upon the accurate prediction by a fractal of the examined structure. Conclusions: Brain vessels can be modeled with the help of fractals with condensation points, which have differentiated one-sided dimensions.

1207/2523

Tytuł oryginału: Immune processes in the pathogenesis of Parkinson's disease - a potential role for microglia and nitric oxide.
Autorzy: Członkowska Anna, Kurkowska-Jastrzębska Iwona, Członkowski Andrzej, Peter Doris, Stefano George B.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.RA165-RA177, il., bibliogr. 174 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: It has been known for many years that immune system alterations occur in Parkinson's disease (PD). Changes in lymphocyte populations cerebrospinal fluid and blood, immunoglobulin synthesis, and cytokine and acute phase protein production have been observed in patients with PD. In this regard, PD patients exhibit a lower frequency of infections and cancer, suggesting that immune system stimulation may occur. This hypothesis is further supported by the observation of T-cell activation leading to the production of interferon ç in PD. As in other CNS degenerataive diseases, in damaged regions in the brains of PD patients, there is evidence of inflammation, characterized by glial reaction (especially microglia), as well as increased expression of HLA-DR antigens, cytokines, and components of complement. These observations suggest that immune system mechanisms are involved in the pathogenesis of neuronal damage in PD. The cellular mechanisms of primary injury in PD have not been clarified, however, but it is likely that mitochondrial mutations, oxidative stress and apoptosis play a role. Furthermore, inflammation initiated by neuronal damage in the striatum and the substantia nigra in PD may aggravate the course of the disease. These observations suggest that treatment with anti-inflammatory drugs may act to slow progression of PD.

1208/2523

Tytuł oryginału: Nitric oxide and pulmonary circulation.
Autorzy: Clini Enrico, Ambrosino Nicolino
Źródło: Med. Sci. Monitor 2002: 8 (8) s.RA178-RA182, bibliogr. 54 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of this review is to outline the characteristics of pulmonary circulation in health and disease and to define the value of exhaled NO (eNO) as a means to assess the involvement of pulmonary cirulation in pathology. The discovery of the endocrine role of the endothelium has generated great interest in its potential role in regulating the vascular tone of the pulmonary vascular bed. Nitric oxide (NO)-mediated, endothelium-dependent relaxation has been demonstrated in the pulmonary arteries of animals and humans. Changes in the NO pathway in pulmonary hypertension are not completely understood. It is clear that NO has an important role in modulating the response to acute hypoxia, increased flow, and shear stress. The amount of exhaled NO (eNO) in different species may be easily measured, reflecting the overall NO metabolism from the lung (thus including epithelial, endothelial and other cell activity). The development of pulmonary hypertension secondary to systemic (systemic sclerosis, chronic heart failure) or pulmonary (COPD) diseases appears to be associated with a decrease in NO production both at rest and during exercise. Chronic inhalation of NO appears to protect against pulmonary hypertension in animal models. Exhaled no is attracting interest for its in vivo ability to represent the features of pulmonary circulation in pathology.

1209/2523

Tytuł oryginału: Three-dimensional structure and molecular mechanism of novel enzymes of spore-forming bacteria.
Autorzy: Jedrzejas Mark J.
Źródło: Med. Sci. Monitor 2002: 8 (8) s.RA183-RA190, il., bibliogr. 38 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Bacillus and Clostridium species are spore-forming bacilli that cause serious diseases in livestock and in humans. An important pathogen in this group of organisms is Bacillus anthracis is which is the causative agent of anthrax. The biochemical properties, structure, function, and mechanism of catalysis of the novel spore germination protease (GPR) that degrades small, acid soluble proteins (SASP) protecting spore's DNA against damage and a novel, cofactor independent phosphoglycerate mutase (iPGM) are described. A coat created from SASP synthesized during sporulation protects DNA in spores and later in the germination this protective coat is removal by a novel GPR protease. GPR does not resemble in its structure any other known protein and functionally likely belongs to a novel class of proteases utilizing glutamic acid residue in its catalysis in order to degrade SASP. This process provides amino acids necessary for synthesis of other proteins or enzymes necessary in vegetative state. In addition during the spore creation process energy is stored in the form of 3-phosphoglycerate by downregulating a novel, diphosphoglycerate independent iPGM. These depots of energy are utilized later by the germinating spore in addition to the above source of amin acids. The two above enzymes are inherently involved in the processes of sporulation, spore germination and outgrowth. A combination of structural investigations and site-directed mutagenesis studies that were used to characterize the functional properties and the active sites of these novel proteins will be discussed. The understanding of...

1210/2523

Tytuł oryginału: Critical appraisal of facet joints injecitons for chronic whiplash.
Autorzy: Kwan Oliver, Friel Jon
Źródło: Med. Sci. Monitor 2002: 8 (8) s.RA191-RA194, bibliogr. 18 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Whiplash has been defined as an injury mechanism, an injury, a medicolegal and social dilemma, and a complex chronic pain syndrome. The chronic whiplash syndrome has long been a source of controversy, in large part because most patients do not have any readily identifable injury in either the acute or chronic stage. Many sites of patholog have been postulated over the years, but all have thus far been unsubstantiated by objective research evidence or have been shown in controlled studies to be no more prevalent in injured than in uninjured subjects. Recent promise has been afforded to the possibility of acute facet joing injury in the cervical spine and chronic pain arising from the facet joint. Despite the great appeal of this notion, however, there are considerable methodological and epidemiological barriers to its acceptance, and thre remains a paucity of research to date to advance this theory. In this review, we examine the clinical studies which have identified facet joint pain in whiplash patients and have examined the diagnosis of the same via facet joint blocks and treatment via radiofrequency neurotomy. We conclude that this therapy should not be used until more credible and scientific evidence arises from further study.

1211/2523

Tytuł oryginału: Ludwik Hirszfeld: a life in serology.
Autorzy: Schneider William H.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.355-359, il.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Hirszfeld

Ludwik 1884-1954

1212/2523

Tytuł oryginału: Ludwik Hirszfeld (1884 - 1954): a personal memoir.
Autorzy: Balińska Marta Aleksandra
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.361-353
Sygnatura GBL: 304,223

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Hirszfeld

Ludwik 1884-1954

1213/2523

Tytuł oryginału: Predicting outcome in hematological stem cell transplantation.
Autorzy: Dickinson Anne M., Cavet James, Cullup Hannah, Wang Xiao N., Jarvis Mark, Sviland Lisbet, Middleton Peter G.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.371-378, il., tab., bibliogr. 62 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

transplantologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

myszy

Streszczenie angielskie: This review summarizes recent results investigating the role of certain cytokine gene polymorphisms, including those of TNF-ŕ, IFN-ç, IL-6, IL-10 and IL-1 receptor antagonist (IL-1Ra), in allogeneic stem cell trasplantation. It discusses their role in predicting outcome and the development of a genetic risk index for graft versus host disease (GvHD) in HLA-matched sibling transplants. By the comparative use of an in vitro human skin explant model, initial results suggest than certain cytokine gene polymorphisms may be associated with more severe disease.

1214/2523

Tytuł oryginału: Structures and serology of the O-specific polysaccharides of bacteria of the genus Citrobacter.
Autorzy: Knirel Yuriy A., Kocharova Nina A., Bystrova Olga V., Katzenellenbogen Ewa, Gamian Andrzej
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.379-391, il., tab., bibliogr. 81 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The review presents the structures of the O-spcific polysaccharides (O-antigens) of the lipopolysaccharides isolated from over 25 Citrobacter strains, which represent different species and serogroups. The correlation between O-antigen structure and immunospecificity as well as numerous cross-reactions between Citrobacter and other enterobacterial species are discussed.

1215/2523

Tytuł oryginału: Streptomyces and Escherichia coli, model organisms for the analysis of the initiation of bacterial chromosome replication.
Autorzy: Messer Walter, Zakrzewska-Czerwińska Jolanta
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.393-398, il., bibliogr. 37 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

mikrobiologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Streptomyces coelicolor A3(2) and Escherichia coli are quite different in their life-style and the structures of their genomes. Streptomyces exhibit complex multicellular development including formation of multigenomic hyphae during growth. These organisms possess a large linear (8.7 Mb) and GC-rich (~72 p.c.) chromosome. The genome sequence of S. coelicolor has just been completed. The difference between E. coli and Streptomyces making them an excellent model organisms for a comparison of their replication modes. In this review, we compare initiation of chromosome replication in both organisms. Their replication origins are different in size, but both have DnaA boxes - a binding motifs for initiator DnaA protein. The two DnaA proteins have practically the same biochemical properties. Many aspects of the control of initiation seem to be similar. A comparison of the two systems thus allows us to define those aspects of replication initiation that are universally used in the eubacterial kingdom.

1216/2523

Tytuł oryginału: Lactoferrin and immunologic dissonance: clinical implications.
Autorzy: Kruzel Marian L., Zimecki Michał
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.399-410, il., tab., bibliogr. 84 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Homeostasis is the maintenance of equilibrium in a biological system by means of positive and negative feedback control mechanisms that counteract influences tending toward physiological dissonance. At the molecular level, homeostasis is controlled by the network of the neuro-endocrine-immune system, in which lactoferrin (LF) plays a central role. The purpose of this review is to provide a comprehensive summary of a collaborative study established between the Hirszfeld Institute of Immunology and Experimental Therapy (Wrocław, Poland) and the University of Texas Helth Science Center (Huston USA) regarding LF and its role in homeostasis. In our studies we focused on the immunoregulatory functions of LF, both in vitro and in vivo. We investigated the immune status of individuals subjected to different isults, including experimental endotoxemia in mice and surgery in humans. We also studied a LF-dependent delayed type hypersensitivity (DTH) response to evaluate some of the mechanisms by which LF can effectively substitute an adjuvant in vaccine.

1217/2523

Tytuł oryginału: Experimental therapies for psoriasis.
Autorzy: Asadullah Khusru, Volk Hans-Dieter, Friedrich Markus, Sterry Wolfram
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.411-420, il., tab., bibliogr. 46 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

dermatologia i wenerologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: There is a high medical need for better therapies for psoriasis. Based on new insight into the pathophysiology of this frequent immune disease, a number of novel systemic immunomodulatory therapies are currently in clinical developmet. These include approaches targeting antigen presentation and costimulation, T cell activation and leukocyte adhesion, action of proinflammatory mediators, and modulating the cytokine balance. Although mainly only preliminary data are available so far, these trials contribute to a further understanding of the disease and will eventually lead to new therapeutic options for psoriasis. Moreover, since psoriasis can be considered as a visible model disease for T cell-mediated disorders characterized by a type 1 cytokine pattern in general, such approaches may have impact for other immune disorders as well. Here we review the rationale and the initial clinical data of these important recent experimental therapies.

1218/2523

Tytuł oryginału: Diminished expression of the type II receptor for TGFá (TGFáRII) in T lymphocytes from patients with sezary syndrome is not due to mutations in the receptor's poly-A tract: limitations of the standard RT-PCR in cDNA sequence analysis of homopolymeric base stretches.
Autorzy: Zhang Qian, Capocasale Renold J., Fox Floyd E., Bedian Vahe, Vonderheid Eric C., Rook Alain, Moore Jonni S., Nowell Peter C., Haines Dale S., Wasik Mariusz A.
Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.421-429, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 304,223

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Perihperal blood lymphocytes from patients with Sezary syndrome (SzS) frequently demonstrate decreased surface expression of transforming growth factor á receptor II (TGFáRII). The mechanism of this low TGFáRII expression remains unknown. Because mutations within the poly-A tract of the TGFáRII sequence (nucleotides 709 - 718) were shown to result in diminished TGFáRII expression in other types of malignant tumors, we examined the sequence of the TGFáRII poly-A tract in two SzS-derived cell lines and in peripheral blood SzS cells from 17 SzS patients and 4 control, healthy individuals using DNA sequencing and single-stranded conformation polymorphism (SSCP) analysis. A standard bidirectional, automated sequence analysis of the RT-PCR-generated cDNA TGFáRII fragment showed a heterogenous population of the normal length, 10-, with admixed, shortened, 9-base poly-A stretches. Surprisingly, this mixture was present not only in the cells from 5 SzS patients and 2 SzS cell lines, but also in cells from 2 healthy control individuals. Importantly, the proportion of the shortened, 9-base fragments was markedly reduced or practically eliminated when the procedure was modified by usage of high-fidelity DNA polymerase, labeled primers and/or cloned RT-PCR products, which indicates that the presence of the shortened, 9-base fragments represented a procedural phenomenon rather than a true deletional mutation within an allele of the TGFáRII gene. Accordingly, SSCP analysis of genomic DNA did not reveal any mutations within the poly-A tract-containing region. These results indicate ...

1219/2523

Tytuł oryginału: In vivo changes in the intestinal lipid peroxidation and the crypt response to whole-body gamma irradiation in diethylnitrosamine-treated mice.
Tytuł polski: Zmiany in vivo w peroksydacji lipidów oraz odpowiedzi krypt jelitowych myszy napromieniowanych na całe ciało promieniowaniem gamma po zatruciu nitrosodietyloaminą.
Autorzy: Grudziński Ireneusz P.
Źródło: Rocz. Państ. Zakł. Hig. 2002: 53 (4) s.333-340, il., bibliogr. 33 poz., streszcz.
Sygnatura GBL: 301,906

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

myszy

Streszczenie polskie: Przeprowadzono badania w celu analizy peroksydacji lipidów oraz przeżycia krypt w błonie śluzowej jelita cienkiego myszy zatruwanych per os N-nitrozodietyloaminą (DENA) (o.01, 0.1, or 1.0 mg kg/m.c) przez okres 21 dni oraz napromieniowanych na całe ciało (2.5, 5.0, 7,5, or 10.0 Gy) promieniowaniem gamma po ekspozycji an DENA. Myszy otrzymujące per os fizjologiczny roztwór chlorku sodowego (0.9 proc. NaCl) stanowiły kontrolę w doświadczeniu. Rezultaty wykazały, że DENA obniża letalną dawkę promieniowania gamma dla komórek krypty jelitowej oraz zwiększa wrażliwość krypt na promieniowanie gamma. Obecne wyniki sugerują, że pro-oksydacyjne oddziaływanie DENA i/lub promieniowania gamma jest czynnikiem ryzyka dla przeżywalności krypt jelita cienkiego.

1220/2523

Tytuł oryginału: NoiseChem: an European Commission research project on the effects of exposure to noise and industrial chemicals on hearing and balance.
Autorzy: Prasher Deepak, Morata Thais, Campo Pierre, Fechter Lawrence, Johnson Ann-Christin, Lund Soren Peter, Pawlas Krystyna, Starck Jukka, Sułkowski Wiesław, Śliwińska-Kowalska Mariola
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.5-11, il., tab., bibliogr. 11 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Exposure to multiple physical and chemical agents is common in occupational environments but workplace hazards and occupational safety critieria for combined exposures are lacking. NoiseCHem is an European Commision research project examining the effects of exposure to noise and chemicals on hearing and balance. Partners in Sweden, Finland, France, Denmark, UK and Poland with expert guidance from partners in the USA will examine workrers and study the mechanisms of action in animals to determine the levels of risk associated with joint exposure to noise and solvents. This paper biriefly outlines the project details.

1221/2523

Tytuł oryginału: Identification and assessment of work-related nervous system lesions.
Autorzy: Langauer-Lewowicka Henryka
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.13-17, bibliogr. 22 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Short historical data on occupational neurotoxicology, as well as on the pathogenic mechanisms of the nervous system impairments due to occupational factors are descirbed. The main difficulties in the assessment of peripheral and central nervous system lesions are stressed.

1222/2523

Tytuł oryginału: F2-isoprostanes biomarkers of lipid peroxidation: their utility in evaluation of oxidative stress induced by toxic agents.
Autorzy: Piłacik Bożena, Wrońska-Nofer Teresa, Wąsowicz Wojciech
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.19-27, il., bibliogr. 74 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

szczury

Streszczenie angielskie: Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of polyunsaturated fatty acids. There is a direct evidence showing that F2-isoprostanes can be utilized as a marker of lipid peroxidation due to the mechanism of their foramtion (nonenzymatic exidation of arachidonic acid), chemical stability, sensitive and non-invasive methods of their estiamtion. An altered generation of F2-isoprostanes has been found in a variety of pathological syndromes associated with oxidative stress. Their quantification allows to elucidate the role of free radicals in oxidative injury. This paper reviews briefly the recent data on isoprostanes: biochemical mechanisms of their formation, methods of their measurement, and the possibilities of their utilization as a quantitative/qualitative marker of oxidative stress in vivo.

1223/2523

Tytuł oryginału: Theoretical assumptions and psychometric characteristics of the sense of personal control at work questionnaire.
Autorzy: Dudek Bohdan, Merecz Dorota, Makowska Zofia
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.29-36, il., tab., bibliogr. 18 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

psychiatria i psychologia

Typ dokumentu:

praca epidemiologiczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Personal control is a very important determinant of stress experiennced at work. This variable is taken int account in different models of stress, and its role in the relationship between stress and stress effects has been studied in many research projects. But there is still lack of in-depth theoretical considerations on various definitions of control, which would allow to develop precise operationalization of this phenomenon. The following issues are discussed in this paper: 1) theoretical assumptions important for proper construction of the Sense of Personal Control at Work (SPCW); 2) the origins of SPCW Questionnaire; 3) the results of empirical study on psychometric charactieristics of the SPCW questionnaire. A group of 160 subjects participated in the study; the SPCW Questionnaire was administserd twice with a 2-week interval; at the first stage, it was used together with other instruments, such as Maslach Burnout Inventory, Stress at Work Questionnaire, Work Satisfaction Scale, general Health Questionnaire-28. Reliaility coefficients: cronbach a = 0.81; rtt = 0.69. Validity: correlation coeffcients between personal control and work satisfaction r = 0.44; stress at work r = -0.33; emotional exhaustion r = -0.28; personal accomplishment r = 0.25; depersonalisation r - -0.26; mental health r = -0.19. Explaratory factors analysis have not confirmed theoretical structure of the SPCW Questionnaire.

1224/2523

Tytuł oryginału: Toxicokinetics and metabolism of pseudocumene (1,2,4-trimethylbenzene) after inhalation exposure in rats.
Autorzy: Świercz Radosław, Rydzyński Konrad, Wąsowicz Wojciech, Majcherek Wanda, Wesołowski Wiktor
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.37-42, il., tab., bibliogr. 8 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

toksykologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The objective of this study was to evaluate the toxicokinetics and metabolism of pseudocumene after inhalation exposure. Male Wistar rats were exposed to pseudocumene vapors at nominal concentrations of 25, 100 or 250 ppm in the dynamic inhalation chambers for 6 h. Blood samples were collected during (between 1st and 6th h) and after exposure (between 6th min and 6th h). Blood concentrations of pseudocumene were estiamted by gas chromatography using the headspace technique. During a six-hour exposure, the concentration of pseudocumene in blood increased rapidly within the first 2 h reaching then a plateau. The elimination of pseudocumene from blood followed an open two-compartment model. Urine samples were collected from the exposed animals, and metabolites were analyzed by gas chromatography with a flame ionization detector. Three metabolites were measured in the rat uring after hydrolysis: 3,4-dimethylbenzoic acid (3,4-DMBA), 2,4-dimethylbenzoid acid (2,4-DMBA) and 2,5-dimethylbenzoic acid (2,5-DMBA). A significant linear correlation was found between the level of exposure and the concentration of dimethylbenzoic acids. The enzyme kinetics of pseudocumene biotransformation was calculated by Lineweaver-Burk equation. Metabolic constants, Km (mg/l) and Vmax (mg/h/kg), the parameters for pseudocumene biotransformation by rats were estiamted (3,4-DMBA-Km = 28, Vmax = 96; 2,4-DMBA - Km = 7, Vmax = 25; 2,5-DMBA - Km = 7, Vmax = 23).

1225/2523

Tytuł oryginału: Risk for early ischemic event after acute myocardial infarction in working males.
Autorzy: Froom Paul, Gofer Dafna, Boyko Valentina, Goldbourt Uri
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.43-48, il., tab., bibliogr. 15 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: We attempt to predict ischemic events in the weeks following the hospital discharge after acute myocaridal infarction (AMI) in order to aid occupational physicians in taking return-to-work decisions. Included in the study were 1299 AMI patients from 14 coronary care units in Israel who had been employed before hospitalization and were seen after discharge 1,2,4,6, 9 and 12 months after the first AMI. Cardiovascular accidnts included death from cardiovascular disease, recurrent infarction or hospitalization due to congestive heart failure or unstable angina. The rate of a first recurrent cardiovascular accident decreased rapidly over the first few weeks. The risk in the third week was still 51 per 1000 person-months, 13.4 (95 p.c. Cl: 9.2 - 19.5) times higher than that after 10 weeks when the incidence reached a low steady state. The major predictors in a logistic regression model for an event were: older age, past history of a cerebrovascular event, and congestive heart failure during the course of hospitalization, but the area under the receiver-operator curve was only 64.4 p.c. We conclude that after acute myocardial infarction, the major risk for a recurrent event is a lapse of tiem from discharge but not other clinical variables. This should be taken into account when considering a proper timing of return-to-work after an acute myocardial infarction.

1226/2523

Tytuł oryginału: Work-related unjuries among firefighters: sites and circumstances of their occurrence.
Autorzy: Szubert Zuzanna, Sobala Wojciech
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.49-55, il., tab., bibliogr. 21 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The aim of the present study was to determine the injury ratio, causes and duration of temporal work disability from on-duty injuries among firefighters, taking into acount the site and circumstances of their occurrence. The study was performed on a representative sample of 1503 firefighters from 29 fire stations who were employed between 1994 and 1997. Subject to investigation were data on the numer of days and cases of work disability due to on-duty injury, personal data (age, work duration) and data on the circumstances of injury during emergency operations, taking part in compulsory physical training, performing maintenance and repair works, on duty at the fire station, and when commuting to or from work. The analysis of work-related causes and cirucmstance of injuries among fireflighters revealed that the majority of injuries (40 p.c.) occurred during compulsory physical training, being responsible for 41 p.c. of post-injury absence at work. The workers employed for less than one year were at highest risk of injury. Injuries during emergency operations made 25 p.c. of all injuries and accounted for 24 p.c. of post-injury absence. The analysis of data showed that the frequency of injuries was not significantly aged-dependent, however, the duration of work disability was found to increase by 20 p.c. with increasing age og workers. The results indicated the need for undertaking preventive intervetions to minimize occupational hazards to those involved in firefighting.

1227/2523

Tytuł oryginału: Evaluation of airborne dust and endotoxin in corn storage and processing facilities in Colorado.
Autorzy: Buchan Roy M., Rijal Pabitra, Sandfort Delvin, Keefe Tom
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.57-64, il., tab., bibliogr. 32 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

pulmonologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: The main objectives of this research were to determine what aerosols were present by taking total dust (TD) samples and thoracic particulate mass samples (TPM) on farms and in grain elevators. Cascade impactors were used to characterize size distributions of dust and endotoxing at each site. Total dust concentrations on farms had a genometric mean 3.4 mg/m3 adn 3.3 mg/m3 in elevators. The geometric mean (GM) concentrations for the TPM were 2.4 mg/m3 on farms and 1.0 mg/m3 in elevators. Endotoxin concentrations as geometric means were alarming at 3175 EU/m3 total dust and 983 EU/m3 by TPM on farms. In elevators, the GM concentrations for endotoxins were 2534 EU/m3 total dust, and 526 EU/m3 by TPM. The mass median aerodynamic diameter (MMAD) for endotoxins on farms was 8.0 ćm and 6.5 ćm in elevators. The paired t-test was applied to the log ratios of endotoxin concentrations (EU/m3) and dust concentrations (mg/m3), for paired samples of the TD and TPM. A higher content of endotoxings was associated with TPM for farms but not elevators. It was concluded that although the TPM fraction (dust) may represent a small part of the total mass, the aerosol size is optimum for deposition in the lung's tubular airways, and might cause airway inflamation due to the endotoxins. The TPM fraction of corn dust represents the best measure of exposure with regard to the potential development of long-term airways inflammation, and the potential of chronic obstructive pulmonary disease among chronically exposed workers. All endotoxin concentrations were well above recommendd exposure levels of several researchers familiar with endotoxin health effects.

1228/2523

Tytuł oryginału: Asbestos in water sources of the Bazhenovskoye chrysotile asbestos deposit.
Autorzy: Kashansky Sergey V., Slyshkina Tatiana V.
Źródło: Int. J. Occup. Med. Environ. Health 2002: 15 (1) s.65-68, tab., bibliogr. 13 poz.
Sygnatura GBL: 306,313

Hasła klasyfikacyjne GBL:

ochrona środowiska

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: The paper provides measurements of asbestos fiber levels in water sources from the area of the Bazhenovskoye chrysotile asbestos deposit. All study water samples contained asestos fibers at concentrations one to three orders below the values standardized in the USA (7ú10**6 fibers/liter). All the identified fibers belonged to chrysotile asbestos and no amphibole asbestos, such as tremolite asbestos, has been identified. The anthropogenic load of asbestos fibers in Asbest City's environment is increasing in the volume of 5.770ú10**f14 fibers/liter or 10.2 kg of chrysotile asbestos. The authors consider it advisable to continue studies to measure asbestos levels in the water sources in the areas located in the vicinity of oter Russian asbestos deposits.

1229/2523

Tytuł oryginału: Alfred Grotjahn ber rzte als Patienten.
Tytuł polski: Alfred Grotjahn o lekarzach jako pacjentach.
Autorzy: Goerke Heinz
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.141-149, bibliogr. [7] poz., streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia nowożytna

Temat osobowy:

Grotjahn

Alfred 1869-1931

Streszczenie polskie: Twórca higieny społecznej jako dyscypliny akademickiej w Niemczech, Alfred Grotjahn (1869-1931), opublikował w roku 1929 zbiór historii choroby, jakie o swoich chorobach sporządzili sami lekarze. Raporty te, Grotjahn zaczerpnął z trzech różnych źródeł: 1) z literatury autobiograficznej, 2) z publikacji kazuistycznych i 3) z badań własnych przeprowadzonych w formie ankiety. Do przeprowadzenia tych studiów skłoniło go przekonanie, iż z reguły brak jest wystarczającego psychologicznego zrozumienia osób wykonujących zawód lekarski dla subiektywnych przeżyć chorych w stosunku do ich choroby, ale także w relacjach z lekarzami i podejmowanymi przez nich działaniami diagnostycznymi i terapeutycznymi. Za celowe uważał uzyskanie wiedzy na temat odczuć własnych i obserwacji samych lekarzy jako chorych, dlatego iż raczej to w ich przypadku, a nie w przypadku osób nie posiadających fachowej wiedzy medycznej należało spodziewać się zarówno trzeźwego jak i obejmującego szczegóły opisu. Jego lekarskie samoobserwacje, jakie udało się pozyskać z literatury autobiograficznej i kazuistycznej, nie przyniosły jednak zbyt wielu informacji. Grotjahn przypuszczał, iż wynikało to ze swego rodzaju nieśmiałości w udzielaniu dokładniejszych informacji dotyczących bardziej intymnych spraw własnej osoby, ale chyba również i z tego, iż niechętnie przechowywano we wspomnieniach choroby jako nieprzyjemne zaburzenia w wykonywaniu zawodu. Inaczej jednak przedstawiały się wyniki rozesłanych przez niego ankiet. Gwarantowały one anonimowość; przedłożono raporty oceniane przez Grotjahna jako szczególnie cenne...

1230/2523

Tytuł oryginału: Konflikte zwischen Patienten und rzten in wrttembergischen Lungenheilanstalten Anfang des 20. Jahrhunderts.
Tytuł polski: Konflikty pomiędzy pacjentami a lekarzami w wirtemberskich zakładach leczenia chorób płuc na początku 20-tego stulecia.
Autorzy: Hhner-Rombach Sylvelyn
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.151-161, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie polskie: Zakłady leczenia chorób płuc borykały się od początku 20-tego stulecia z różnorodnymi problemami ze swymi męskimi pacjentami, dla których trzymiesięczny pobyt ze ściśle określonymi normami zachowania był przyczyną wielu niesnasek. U niektórych pacjentów doprowadziły one do przedwczesnego przerwania postępowania leczniczego, do opuszczenia zakładów bez zezwolenia i do innych wykroczeń w stosunku do reguł zakładów. Próbując utrzymać dyscyplinę medyczną kierownictwo zakładów sięgało po środek polegający na zwolnieniu. Przyczyny takiego zachowania i reakcji na nie zostaną przedstawione na przykładzie zakładów leczenia chorób płuc w Wirtembergii na początku 20-tego stulecia. W centrum zainteresowania pozostaną pacjenci posiadający ubezpieczenie społeczne, których pobyt w zakładach leczniczych opłacany był przez Krajowy Zakład Ubezpieczeń w Wirtembergii. Pod koniec 19-tego i na początku 20-tego stulecia punktem spornym między pacjentami zakładów leczniczych, a lekarzami było w kwestiach terapeutycznych i diagnostycznych stosowanie tuberkuliny. Zaledwie kilka lat po tak zwanej "euforii tuberkulinowej", pacjenci odmawiali jej przyjmowania lub mieli do niej nieufny stosunek. Pacjenci wywodzący się w komplecie z warstwy robotniczej, okazali się być w sytuacjach konfliktowych z lekarzami jednostkami świadomymi wiedzącymi przynajmniej dokładnie, czego nie chcą.

1231/2523

Tytuł oryginału: Literaten des 19. Jahrhunderts und ihre Sicht auf Zahnheilkundliches und den Zahnarzt.
Tytuł polski: Literatura 19-stego stulecia i jej spojrzenie na leczenie stomatologiczne i lekarzy stomatologów.
Autorzy: Heidel Caris-Petra, Peikert Sylvia
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.163-178, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Hasła klasyfikacyjne GBL:

stomatologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XIX wieku

Streszczenie polskie: Zdrowie i choroba, czynności lekarskie i wreszcie lekarz jako wiodące wątki w literaturze pięknej stanowiły - nie tylko w najnowszych czasach - przedmiot badań zarówno historyczno-literackich i historyczno-społecznych jak i dotyczących historii medycyny. Mimo to jednak poważni autorzy dochodzą do przekonania, iż na polu literatury na możliwe zbadanie w kontekście historyczno-medycznym czeka jeszcze bardzo obszerny materiał źródłowy. O wiele bardziej krytycznie należy w związku z tym potraktować sytuację dotyczącą opracowania tematu leczenia stomatologicznego w literaturze pięknej. Pominąwszy fakt, iż znanych jest stosunkowo niewiele dzieł literackich podejmujących tematykę leczenia stomatologicznego lub lekarzy stomatologów lub też fakt, iż prawie nie ma dostępnych badań historycznych w tym zakresie, to dotychczas w szczególności nie poddano systematycznej analizie wpływu osobistych doświadczeń literatów ze stomatologią i lekarzem stomatologiem na ich dzieła. Po analizie dzienników, notatek o charakterze osobistym, listów i wspomnień przede wszystkim literatów 19-tego i początku 20-tego stulecia takich jak Heinrich Heine, Friedrich Hebbel i Thomas Mann można wyciągnąć w tej kwestii jednak dość jednoznaczne wnioski. Przeżyte w bardzo różnych uwarunkowaniach i okolicznościach spotkania wymienionych wyżej literatów z lekarzami stomatologami i częściowo długotrwałe i bolesne doświadczenia ze schorzeniami zębów i leczeniem stomatologicznym, znalazły w końcu także swój wyraz w ich dziele literackim. Dowodem tego są zarówno stosunkowo dokładne opisy praktyk stomatologicznych jak i stosunkowo różne prezentacje osoby i roli lekarza stomatologa przedstawiane w różnym czasie przez jednego i tego samego autora.

1232/2523

Tytuł oryginału: Philipp Melanchthon (1497-1560) und die akademische Medizin in Wittenberg.
Tytuł polski: Philipp Melanchthon (1497-1560) i medycyna akademicka w Wittenberdze.
Autorzy: Helm Jrgen
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.179-196, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XVI wieku

Temat osobowy:

Melanchthon

Philipp 1497-1560

Streszczenie polskie: Stosunek Philippa Melanchthona do medycyny akademickiej jego epoki wielokrotnie stanowił w minionych latach przedmiot studiów medyczno-historycznych. Wykład podsumowuje najistotniejsze wyniki tych prac i pokazuje, czy i w jaki sposób propgram odnowy medycyny Melanchthona wywarł wpływ na zajęcia medyczne na Leucorea. Ponadto w oparciu o porównanie Uniwersytetu w Wittenberdze i katolickiego Uniwersytetu w Ingolstadt, poddaje analizie przypuszczenie wyrażane w nowszych pracach, niż w medycynie akademickiej 16-tego stulecia (również wskutek wpływu Melanchthona) różnice uwarunkowane wyznaniem. Prezentacja ta w formie uroczystego wykładu wypełnia ramy przypadającego na rok 2002 jubileuszu 500-lecia utworzenia Uniwersytetu w Wittenberdze.

1233/2523

Tytuł oryginału: Selektion und Attraktion. Patienten im klinischen Versuch im frhen 19. Jahrhundert.
Tytuł polski: Dobór i atrakcja. Pacjenci w eksperymentach klinicznych w początkach 19-tego stulecia.
Autorzy: Hess Volker, Thoms Ulrike
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.197-207, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XIX wieku

Streszczenie polskie: Swobodny dostęp do pacjentów uważany jest w współczesnym piśmiennictwie historii społecznej za istotny warunek powstania nowoczesnej medycyny szpitalnej. Dotyczy to w szczególności rozwoju eksperymentu klinicznego stanowiącego dzisiaj metodyczny standard nauk klinicznych. Podczas gdy wielokrotnie w ostatnich latach podejmowano kwestię dowolności i uzyskania przyzwolenia chorego w okresie po roku 1850, to wyraźnie brak jest jeszcze studiów empirycznych dotyczących doboru i dostępności pacjentów w początkach 19-tego stulecia. Wgląd w praktykę takich eksperymentów dają źródła archiwalne o eksperymentach klinicznych przeprowadzonych w Charit w Berlinie w okresie od 1800 roku do roku 1850. Zawierają one również petycje osób proszących pilnie o dopuszczenie do eksperymentów. W tym kontekście wykład ten próbuje rzucić światło na wzajemne relacje wyznaczone z jednej strony kryteriami doboru medycznego i z drugiej pragnieniem uczestnictwa w eksperymentach, a być może uzyskania jakiś korzyści dla siebie samego. W oparciu o jakie kryteria medyczne dokonywano wyboru pacjentów? Czy w grupie wybranych osobników badanych skonstatować można jakieś cechy wspólne i jakie one miały charakter? Co skłaniało ludzi do błagania o przyjęcie do szeregu uczestników eksperymentu?

1234/2523

Tytuł oryginału: Leprakranke im Spiegel sptmittelalterlich-frhneuzeitlicher Schauprotokolle und Selbstzeugnisse.
Tytuł polski: Trędowaci w świetle późnośredniowiecznych i wczesnonowożytnych dokumentów i w świadectwach własnych.
Autorzy: Jankrift Kay Peter
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.209-217, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Hasła klasyfikacyjne GBL:

choroby zakaźne

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia średniowiecza

Streszczenie polskie: Żadna inna choroba nie poddawała ludzi z epoki późnego średniowiecza i wczesnych czasów nowożytnych takiemu zakresowi decydowania o ich losie jak "trąd" - choroba tak określana przez współczesnych. Do okresu późnego średniowiecza na terenie Rzeszy Niemieckiej wytworzyły się różne centra - oględzin, których pozytywne orzeczenie powodowało radykalne konsekwencje dla dalszego losu osoby podejrzanej o trąd: utratę dotychczasowego otoczenia i przystąpienie do wspólnoty "żyjących" zmarłych w jakimś leprozorium. Informacje o traktowaniu osób podejrzanych o trąd i chorych na trąd ukazane są w świetle dokumentów i raportów oględzinowych poszczególnych zakładów dla trędowatych. Niuanse spowodowane są rodzajem badań wykonywanych z jednej strony przez samych trędowatych mistrzów, a z drugiej strony od 15-tego stulecia na niemieckim obszarze językowym w coraz znaczniejszym stopniu przez lekarzy i chirurgów. Szeroki wgląd w środowisko społeczne badanych, tło podejrzenia o występowanie trądu i orzeczenia umożliwiają zachowane w znacznej ilości dokumenty Wydziału Medycznego Uniwersytetu w Kolonii, uzupełnione w ramach niniejszego wystąpienia kolejnymi świadectwami, przykładowo z Lttich. Światło na samoodbiór trędowatych i podejrzanych o trąd rzucają podania o przyjęcie, jak na przykład Josta Heerde, przyjętego do lepozorium w Mnstwer na początku 17-go stulecia, a także wypowiedzi mieszkańców zakładu dla trędowatych przy okazji wizytacji lekarskich, na przykład z Paderborn w Westfalii.

1235/2523

Tytuł oryginału: Arzt und Patient im Lichte preussischer Medizinal-Edikte fr Schlesien.
Tytuł polski: Lekarz a pacjent w świetle pruskich edyktów medycznych dla Śląska.
Autorzy: Jonecko Antoni
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.251-279, il., bibliogr. 43 poz., streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Hasła klasyfikacyjne GBL:

prawo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XVIII wieku

historia XIX wieku

Temat korporatywny:

Śląsk

Streszczenie polskie: Po zajęciu Śląska przez Prusy w XVIII wieku, ustanowiono zrazu "Komisariat Wojenny". Następnie utworzono "Izby dla Spraw Wojny i Domen". Przy izbach istniało "Kolegium Medyczne", ktore zajmowało sie polityką zdrowotną, szkoleniem i rozstrzygało spory pomiędzy lekarzami, chirurgami i aptekarzami. Należy odróżnić: 1) ustrój Śląska staro-pruski, który trwał od zajęcia Śląska w 1742 roku, aż do lat 1806-1812, - oraz 2) ustrój nowo-pruski Śląska, wieku XIX. Przedstawiona praca zajmuje się pruskimi sprawami medycznymi głównie pierwszego okresu. Władze pruskie Śląska wydały drukiem bardzo wiele edyktów, regulaminów i patentów o treści medycznej i socjalnej. Drukowano je w różnych oficynach i różnych miastach. Rozporządzenia te były wydawane w języku niemieckim i również w języku polskim. Dokumenty te pozwalają na określenie stosunku lekarza do pacjenta i stosunku absolutystycznego państwa do ludności. Pruska polityka zdrowotna zajmowała się głównie epidemiami, wakcynacją, modnym wówczas zagadnieniem śmierci pozornej i reanimacji, problemami osób kalekich, ubogich, tudzież weteranami, sierotami i wdowami wojennymi, zbiegami oraz tzw. "Domami Poprawy". Największym zainteresowaniem urzędowym cieszyły się rzemieślniczo uprawiane zawody chirurgów, położnych i aptekarzy. Władze pruskie dobrze rozpoznały dużą przydatność i wydolność tych profesji i przyczyniły się do doskonalenia i awansu tych zawodów. Edykty dla Śląska, dla częściowo autonomicznego "Księstwa Śląskiego i Hrabstwa Kłodzkiego" były sygnowane przez króla Fryderyka II, Fryderyka Wilhelma I, Fryderyka Wilhelma II lub Fryderyka Wilhelma III...

1236/2523

Tytuł oryginału: Der Chemiker Ragnar Berg (1873-1956) und sein Beitrag zur Ernhrungsforschung.
Tytuł polski: Chemik Ragnar Berg (1873-1956) i jego wkład do badań nad odżywianiem.
Autorzy: Lienert Marina, Rummel Christian
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.311-313, bibliogr. 1 poz. streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XX wieku

Temat osobowy:

Berg

Ragnar 1873-1956

Streszczenie polskie: Doniesienie zajmuje się zwięzłym przedstawianiem osiągnięć badawczych szwedzkiego chemika Ragnara Berga. Jest on znany w związku z jego teorią o odżywianiu nadmiernie zasadowym i odżywianiu nadmiernie kwasorodnym. W zakresie praktyki żywieniowej Berg stwierdził, iż gotowanie jarzyn doprowadza do nadmuiernych strat cennych składników mineralnych. Dlatego zalecał i wprowadził technikę parowania, zamiast niekorzystnego gotowania. Po ukazaniu się monografii Kazimierza Funka, o witaminach w roku 1914, Ranger Berg opublikował drugą z kolei monografię na temat witamin. Berg upamiętnił się w historii techniki żywieniowej poprzez swoje niezwykle ważne i pracochłonne badania nad pierwiastkami śladowymi.

1237/2523

Tytuł oryginału: ltere Patienten in der Medizinischen Klinik der Universitt Tbingen 1880-1914.
Tytuł polski: Starsi pacjenci hospitalizowani w Klinice Medycznej Uniwersytetu w Tybindze w latach 1880-1914.
Autorzy: Moses Simone
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.315-326, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: W wykładzie zostaną zaprezentowane wyniki analizy ilościowej pacjentów powyżej 55 roku życia w podanym przedziale czasowym. Wykorzystane źródła wyjaśniają kwestie związane z łączną liczbą pacjentów rocznie, ich płcią, wiekiem, zawodem, miejscem zamieszkania i wyznaniem, terminem przyjęcia do szpitala. Wskazują też na to, czy pacjent zmarł w szpitalu. Stwierdzić można, kto płacił za pobyt w klinice i czy ten sam pacjent znalazł się w szpitalu wielokrotnie. Częściowo znaleść też można informacje o zdiagnozowanych chorobach. Istotnym czynnikiem zwiększenia liczby pacjentów starszych w szpitalach było wprowadzenie w latach 1880/90 ustawowego ubezpieczenia zdrowotnego, wypadkowego i na wypadek inwalidztwa. W badanym okresie udział bezpłatnie przyjmowanych do szpitala biednych chorych malał na korzyść pacjentów dysponujących teraz ubezpieczeniem społecznym. Klinika uniwersytecka cieszyła się również dobrą opinią wśród bardziej zamożnych pacjentów, jako że ich udział wśród chorych kliniki medycznej był zawsze stosunkowo wysoki i wzrósł nawet po przełomie wieków. Chociaż starsi pacjenci stawiali wysokie wymagania instytucji medycznej, to spadające wskaźniki umieralności podczas badanego okresu wskazują na coraz lepsze możliwości leczenia.

1238/2523

Tytuł oryginału: Therapeutische Effizienz und subjektive Krankheitserfahrung im 18. Jahrhundert.
Tytuł polski: Terapeutyczna efektywność a subiektywne doświadczenie chorobowe w 18-tym stuleciu.
Autorzy: Stolberg Michael
Źródło: Arch. Hist. Filoz. Med. 2002: 65 (2/3) : VIII Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny "Chory między samostanowieniem a paternalizmem" s.375-381, streszcz. - 8 Wspólna Konferencja Polsko-Niemieckiego Towarzystwa Historii Medycyny pt. Chory między samostanowieniem a paternalizmem Wittenberga 06-08.09. 2001
Sygnatura GBL: 301,366

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

historia XVIII wieku

Streszczenie polskie: Lekarze i historycy medycyny ze zdumieniem coraz częściej konstatują dominującą rolę i dużą siłę bezwladności w odniesieniu do upustu krwi, środków przeczyszczających oraz innych środków i metod stosowanych w medycynie minionych epok. Są to bowiem rozwiązania terapeutyczne, które wedle dzisiejszego stanu wiedzy były nieskuteczne lub nawet szkodliwe. W moim wykładzie chciałbym pominąć kwestię "rzeczywistej" skuteczności z dzisiejszej perspektywy. Chciałbym skoncentrować się głównie na zbadaniu, jak laicy medyczni 18-tego stulecia oceniali i odbierali skuteczność leczenia przez lekarzy. Za źródło posłużą mi setki listów pacjentów, a mówiąc dokładniej francuskich i niemieckich konsultacji listownych przeprowadzonych przez laików. Badam je obecnie w ramach zakrojonego na szerszą skalę projektu badawczego. Pacjenci lub członkowie ich rodzin lub też ich znajomi obszernie informują o sukcesach przepisów lekarskich, zarówno z perspektywy dotychczasowej historii jaki i w odniesieniu do wcześniejszych zaleceń terapeutycznych lekarza, który przeprowadził listowną konsultację. Nie pozostawjają przy tym jakichkolwiek wątpliwości, że wspomniane środki i sposoby leczenia uważali za skuteczne. Liczni pacjenci prezentują bardzo konkretną poprawę ich stanu fizycznego po zastosowaniu upustu krwi, środków przeczyszczających lub tylko kąpieli. Ze swej strony domagali się zastosowania odpowiedniego leczenia lub ośwadczali o rezygnacji z niego z uwagi na jego zły przebieg. Listy dokumentują jednak również liczne przypadki niepowodzeń terapeutycznych i jednocześnie wskazują na różne wyjaśnienia, jakie laicy medycznmi znajdowali dla wytłumaczenia tych niepowodzeń, nie poddając w wątpliwość ważności i skuteczności terapii zwolenników teorii humoralnej.

1239/2523

Tytuł oryginału: The results of postoperative irradiation for locally advanced carcinoma of the larynx.
Tytuł polski: Ocena wyników pooperacyjnego napromieniania chorych na miejscowo zaawansowanego raka krtani.
Autorzy: Wieczorek Andrzej, Fijuth Jacek, Michalski Wojciech
Źródło: Nowotwory 2002: 52 (4) s.287-294, il., tab., bibliogr. 38 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie polskie: Wstęp. Celem pracy był retrospektywna anlaiza wyników pooperacyjnej radioterapii u chorych na zaawansowanego raka krtani oraz ocena wpływu czynników prognostycznych klinicznych i terapeutycznych na rokowanie. Materiał. Materiał kliniczny stanowiła grupa 165 chorych na miejscowo zaawansowanego raka krtani, leczonych w latach 1985-1996, u których przeprowadzono uzupełniające pooperacyjne napromienianie. Wszyscy chorzy mieli wcześniej wykonany zabieg operacyjny, polegający na całkowitym usunięciu krtani, połączonym w 91 przypadkach z jednostronną lub obustronną operacją węzłów chłonnych szyi. Czas od operacji do rozpczęcia naporomieniania po operacji wyniósł od 15 do 162 dni (średnia 53). Dawka całkowita wahała się od 50 Gy do 70 Gy przy dawce frakcyjnej 2 Gy. Analizie poddano czas przeżycia oraz czas do miejscowego nawrotu. Prawdopodobieństwo przeżycia obliczano metodą Kaplana-Meiera. Zbadano wpływ: płci, wieku, stopnia, sprawności, stopni zaawansowania T i N, lokalizacji nowotworu, poziomu hemoglobiny, stopnia zróżnicowania histologicznego, zajęcia usuniętych węzłów chłonnych, przejścia nacieku poza torebkę węzła, stopnia doszczętności zabiegu operacyjnego, obecności niekorzystnych cech rokowniczych, związanych z rozrostem guza pierwotnego (zejście pod głośnię, przejście nacieku poza granice anatomiczne krtani, naciekanie chrząstek lub kombinacja powyższych cech) oraz czas od operacji do rozpoczęcia radioterapii na czas przeżycia oraz czas do wystąpienia miejscowego nawrotu, posługując się modelem proporcjonalneg ryzyka D. R. Cox'a...

1240/2523

Tytuł oryginału: Anastomosis site - a risk factor for anastomotic leakage after elective anterior resection of rectal cancer.
Tytuł polski: Wysokość zespolenia jako czynnik ryzyka nieszczelności u chorych po elektywnej resekcji przedniej z powodu raka odbytnicy.
Autorzy: Rutkowski Andrzej, Nowacki Marek P., Olędzki Janusz, Bujko Krzysztof
Źródło: Nowotwory 2002: 52 (4) s.295-299, tab., bibliogr. 24 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Celem pracy było ustalenie zależności pomiędzy wysokością zespolenia okrężniczo-odbytniczego, a ryzykiem nieszczelności i reoperacji. Badano również zależność pomiędzy odległością guza od brzegu odbytu, a szansą na wykonanie operacji oszczędzającej zwieracze. Metoda. Oceniono retrospektywnie 301 chorych, u których wykonano elektywną resekcję przednią z powodu raka odbytnicy. Z badania wyłączono pacjentów, u których występowały naglące wskazania do operacji oraz będących w złym stanie ogólnym, z niedokrwistością lub cukrzycą insulinozależną, o ponad 10-letnim przebiegu. Oceniono ryzyko występowania objawowej nieszczelności. Nieszczelność zespolenia podejrzewano w przypadku objawów rozlanego zapalenia otrzewnej, ropnia w miednicy, obecności gazu lub ropy w drenie. Podejrzenie to weryfikowano badaniem per rectum, ednoskopowo, badaniami obrazowymi oraz oceniano śródoperacyjnie, jeżeli stan chorego wymagał reoperacji. Analizy statystycznej dokonano przy pomocy testu chi-kwadrat przy założonym progu znamienności p 0,05. Wyniki. Wczesne powikłania pooperacyjne (do 30 dni od zabiegu) wystąpiły u 94 chorych (31,2 proc.). Zmarło 2 pacjentów (0,7 proc.). Odsetek nieszczelności zespoleń wyniósł 13,6 proc. Największe ryzyko nieszczelności stwierdzono wówczas gdy dolna granica guza znajdowała się w odległości od 5 cm (26,5 proc.). Przy takim umiejscowieni, odsetek operacji oszczędzających zwieracze był mały (33,1 proc.). Gdy dolna granica guza znajdowałą się w środkowej części odbytnicy, ale poniżej załąmka otrzewnej (powyżej 5 cm do 7-8 cm od brzegu odbytu), ryzyko nieszczelności zespolenia wynosiło 17,3 proc...

1241/2523

Tytuł oryginału: Results of treatment with concurrent radiotherapy and cisplatin-based chemotherapy for cancer of the uterine cervix - a preliminary assessment.
Tytuł polski: Wstępna ocena wyników leczenia raka szyjki macicy napromienianiem skojarzonym z chemioterapią opartą na cisplatynie.
Autorzy: Rusiecka Małgorzata, Ziemba Beata, Dryl Jacek, Bojarowska Katarzyna, Słocka Beata
Źródło: Nowotwory 2002: 52 (4) s.301-304, tab., bibliogr. 18 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Cel. Badanie podjęto w celu wykazania różnicy w wynikach leczenia radiochemioterapią i radioterapią, oraz wskazania metody skuteczniejszej w leczeniu raka szyjki macicy. Materiał i metoda. Badaniem objęto 53 chore na płaskonabłonkowego raka szyjki macicy, poddane radiochemioterapii w okresie od maja 2000 r. do czerwca 2001 r. z powodu nowotworu zaawansowanego miejscowo: stadia kliniczne od IB-2 do IVA - grupa C. Grupę kontrolną stanowiło 50 kobiet z rozpoznanym rakiem szyjki macicy, w stadiach zaawansowania klinicznego od IB-1 do IVA, leczonych wyłącznie radioterapią w 1997 roku - grupa R. Obie grupy były porównywalne pod względem wieku, warunków napromieniania, rodzaju brachyterapii. W grupie C znalazło się więcej chorych z zaawansowanym nowotworem (bulky, oraz przewaga liczbena wyższych stadiów klinicznych). W grupie tej zastosowano wlewy cisplatyny 1 raz w tygodniu w dawce 40 mg/mý, lecz nie więcej niż 70 mg na 1 wlew. Z powodu masywnego krwawienia z dróg rodnych 6 chorych napromieniono metodą hiperfracji: 2 x 1,2 Gy z przerwą 8 h, aby nie stosować hipofrakcjonacji hemostatycznej. Wyniki. U wszystkich chorych grupy C zaobserwowano pozytywny efekt terapii, w tym ponad 50 proc. całkowitych remisji. U ponad 70 proc. kobiet stosowano sterydy z powodu leukopenii, a 4 chore nie otrzymały pełnej chemioterapii - 6 kursów cisplatyny, z powodu trombocytopenii, które wydaje się być największym problemem leczenia skojarzonego. Ilość odczynów poprowmiennych wzrosła, lecz nie wydłużyła się w czasie, ani nie osiągnęła większego natężenia. Podsumowanie. Wczesne wyniki skojarzonego leczenia radiochemioterapią płąskonabłonkowego raka szyjki macicy są bardzo obiecujące i rekomendują powyższy schemat jako standard terapeutyczny w tym schorzeniu.

1242/2523

Tytuł oryginału: Magnetic resonance imaging (MRI) in cervical carcinoma - assessment of diagnostic value of tumour volume.
Tytuł polski: Badanie metodą rezonansu magnetycznego raka szyjki macicy - ocena wartości diagnostycznej objętości guza.
Autorzy: Tacikowska Małgorzata, Grzesiakowska Urszula, Sobiczewski Piotr, Tacikowski Tadeusz
Źródło: Nowotwory 2002: 52 (4) s.305-308, tab., bibliogr. 10 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Badanie metodą rezonansu magnetycznego (MR) odgrywa kluczową rolę w diagnostyce raka szyjki macicy. Jedną z ważnych części badania MR jest pomiar objętości guza. Cel. 1) ocena zgodności pomiaru objętości w badaniu MR z pooperacyjnym badaniem histopatologicznym; 2) ocena korelacji objętości i największego wymiaru raka szyjki macicy (ocenianych w badaniu MR) z następującymi parametrami: a) stopniem zaawansowania według klasyfikacji FIGO; b) naciekaniem przymacicza; c) przerzutami do węzłów chłonnych. Materiał i metoda. Materiał stanowią badania MR miednicy, wykonane aparatem firmy Elscint 2T, u 21 chorych na raka szyjki macicy. Wiek chorych od 37 do 73 lat. U 15 pacjentek po badaniu MR przeprowadzono operację usunięcia macicy, w pozostałych przypadkach rozpoznanie postawiono na podstawie biopsji. U 10 chorych operowanych dokonano oceny porównawczej objętości guza, ocenianej w badaniach MR i histopatologicznym. U 16 chorych analizowano korelację objętości guza ze stopniem zaawansowania, naciekanim przymacicza i powiększeniem węzłów chłonnych. Wnioski. 1) stwierdzono wysoką zgodnośc badania MR i histopatologicznego (po operacji) w pomiarze objętości guza, p = 0.764; 2) badanie MR z pomiarem objętości guza u chorych z rakiem szyjki macicy odznacza się 100 proc. czułością i specyficznością w rozgraniczeniu stopnia I od pozostałych; 3) ocena objętośći guza lepiej koreluje ze stopniem zaawansowania I i II wg FIGO niż ocena największego wymiaru zmiany; 4) stwierdzono korelację pomiędzy objętością guza i naciekaniem przymacicza; 4) nie stwierdzono korelacji objętości guza z przerzutami do węzłów chłonnych.

1243/2523

Tytuł oryginału: Successful use of activated recombinant factor FVIIa in the management of intra abdominal haemorrhage after cytoreductive surgery for advanced carcinoma of the ovary - a case report.
Tytuł polski: Zastosowanie rekombinowanego czynnika krzepnięcia FVIIa w leczeniu ciężkiego krwotoku dootrzewnowego u chorej po rozległej operacji cytoredukcyjnej z powodu zaawansowanego raka jajnika - opis przypadku.
Autorzy: Panek Grzegorz, Derlatka Paweł, Bidziński Mariusz, Stachurska Elżbieta, Krynicki Ryszard
Źródło: Nowotwory 2002: 52 (4) s.309-311, tab., bibliogr. 9 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć żeńska

Streszczenie polskie: Chirurgia cytoredukcyjna stanowi podstawę leczenia zaawansowanego raka jajnika. Towarzysząca tego typu operacjom utrata krwi oraz rozległe uszkodzenia tkanek mogą wyzwolić patologiczne procesy wykrzepiania i fibrynolizy, które w niektórych sytuacjach mogą stanowić bezpośrednie zagrożenie życia chorej. Przedstawiono szczegółowy opis przypadku skutecznego zastosowania rekombinowanego czynnika VIIa u chorej z ciężkim krwawieniem dootrzewnowym, po operacji cytoredukcyjnej, wyknywanej z powodu zaawansowanego raka jajnika. U chorej bez pierwotnych zaburzeń układu krzepnięcia krwi zastosowano dwukrotnie preparat NovoSeven w dawce 20 ćg/kg, uzyskując zatrzymanie krwawienia zagrażającego życiu. Korzystny efekt działania tego preparatu wskazuje na możliwość rozważenia jego użycia w przypadku ciężkich krwawień, niemożliwych do opanowania klasycznymi metodami.

1244/2523

Tytuł oryginału: Chondrosarcoma of the larynx: a case report and review of the literature.
Tytuł polski: Chrzęstniakomięsak krtani; opis przypadku i przegląd literatury.
Autorzy: Jackiewicz Piotr, Kawecki Andrzej, Nasierowska-Guttmejer Anna, Krajewski Romuald
Źródło: Nowotwory 2002: 52 (4) s.312-315, il., bibliogr. 14 poz., streszcz.
Sygnatura GBL: 313,490

Hasła klasyfikacyjne GBL:

otorynolaryngologia

Typ dokumentu:

praca kazuistyczna

Wskaźnik treści:

płeć męska

Streszczenie polskie: Chrzęstniakomięsak (chondrosarcoma) jest rzadko występującym nowotworem krtani. Do tej pory w literaturze opiasno około 250 przypadków chrzęstniakomięsaka w tej lokalizacji. W niniejszej pracy przedstawiono przypadek 46-letniego mężczyzny, u którego w oparciu o badanie kliniczne i histiopatologiczne rozpoznano wysokodojrzałego chrzęstniakomięsaka krtani. Przeprowadzono radykalne leczenie operacyjne, polegające na laryngektomii. Badanie mikroskopowe materiału operacyjnego potwierdziło rozpoznanie chrzęstniakomięsaka o budowie mieszanej, złożonej z chrzęstniaka i chrzęstniakomięsaka, rozwijającego się w chrząstce pierścieniowatej. Ocena marginesów chirurgicznych wykazała całkowite wycięcie guza. W oparciu o przegląd literatury uzasadniono konieczność wykonania radykalnej laryngektomii w przypadku zaawansowanego chrzęstniakomięsaka krtani. Omówiono także problem związany z diagnozą guza oraz próbami wykonywania operacji oszczędzających. Takie czynniki jak: rzadkie występowanie choroby, powolny i skryty wzrost guza oraz obraz histopatologiczny przypominający chrzęstniaka utrudniają postawienie właściwego rozpoznania. Operacje oszczędzające, polegające na częściowej laryngektomii, powinny być zawsze brane pod uwagę. Nie ma jednak jeszcze wypracowanych kryteriów, które pozwoliłyby na zastosowanie leczenia, gwarantującego najlepsze rezultaty funkcjonalne i onkologiczne. Kryteria te powinny opierać się na stopniu zaawansowania klinicznego i stopniu dojrzałości histopatologciznej. W celu opracowania optymalnego leczenia konieczne są dalsze badania kliniczne.

1245/2523

Tytuł oryginału: Long-chain PUFA supplementation improves PUFA profile in infants with cholestasis.
Autorzy: Socha Piotr, Koletzko Berthold, Jankowska Irena, Pawłowska Joanna, Demmelmair Hans, Stolarczyk Anna, Świątkowska Elżbieta, Socha Jerzy
Źródło: Lipids 2002: 37 (10) s.953-957, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 305,815

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

niemowlęta

Streszczenie angielskie: Long-chaim PUFA (LCP) deficiency is a frequent complication in cholestic infants. We investigated the effects of LCP-supplemented formula on EFA status in infants with cholestasis. Twenty-three infants with cholestasis (biliary atresia after surgery, 8; intrahepatic cholestasis, 15) aged 1.9 to 4.9 mon (median 3.1 mon) were randomized to receive commercial infant formulas either without LCP or with LCP from egg phospholipids for 1 mon. Liver tests, nutrient intakes, and plasma phospholipid FA (p.c. w/w) were determined at baseline and after interavention. At baseline, patietns had high serum direct bilirubin levels (5.9 ń 3.0 mg/dL; mean ń SD), they were malnourished (body fat mass: 40 ń 13 p.c. of normal) and presented with PUFA deficiency [plasma phospholipid PUFA: 28.43 p.c. w/w (26.56 - 30.53) in patients vs. 37.02 p.c. w/w (34.53 - 39.58) in controls; median (f1st - 3rd quartile)] with elevated Mead acid and palmitoleic acid. LCP-supplemented (n = 11) and -nonsupplemented groups (n = 12) did not differ in age, indicators of liver function, and EFA status at baseline. After the intervention, LCP-supplemented infants had higher levels of arachidonic acid [7.2 (5.9 - 8.8) vs. 4.2 (f3.0 - 5.3) p.c. w/w; P 0.001] and DHA [2.8 (2.2 - 3.2) vs. 1.6 (1.0 - 2.1) p.c. w/w; P 0.05], accompanied by increased TBARS concentration: 1.9 (1.4 - 2.2) vs 1.3 (1.1 - 1.6) nmol/mL; P 0.05]. We concluded that LCP-supplemented formulae improve LCP status of infants with severe cholestasis but may enhance lipid peroxidation.

1246/2523

Tytuł oryginału: Long-term results of lower limb lengthening by physeal distraction.
Autorzy: Zarzycki Daniel, Tesiorowski Maciej, Zarzycka Maja, Kacki Wojciech, Jasiewicz Barbara
Źródło: J. Pediatr. Orthop. 2002: 22 (3) s.367-370, il., tab., bibligr. 15 poz.
Sygnatura GBL: 313,480

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Of the many methods of limb elongation, lengthening by physeal distraction is among the newest. The goal of this study was to estimate the long-term results of femoral and tibial lengthening by epiphyseal distraction. The authors examined 40 patients who underwent 24 femoral and 16 tibial lengthenings. The average follow-up period was 9.6 years. An average of 4.6 cm femoral lengthening and 4.7 cm of tibial lengthening was achieved. The femoral healing index was 38.1 days/cm and the tibial healing index was 35.7 days/cm. After lengthening the epiphyseal cartilage started to functin in all patients, and premature growth cartilage fusion was not observed during follow-up. Complications occurred in eight patients during femoral lengthening and in four during tibial lengthening. The authors suggest that physeal distraction is a good method for moderate and simple limb lengthening. Particular care should be applied to knee function, especially during femoral lengthening.

1247/2523

Tytuł oryginału: Long-term results of lower limb lengthening by the Wagner method.
Autorzy: Zarzycki Daniel, Tesiorowski Maciej, Zarzycka Maja, Kacki Wojciech, Jasiewicz Barbara
Źródło: J. Pediatr. Orthop. 2002: 22 (3) s.371-374, il., tab., bibligr. 19 poz.
Sygnatura GBL: 313,480

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Methods of limb lengthening have been continously modified. The construction of an apparatus for gradual bone distraction was a great improvement. The aim of this study was to evaluate the long-term results of lower limb elongation using the Wagner technique. Between 1983 and 1989, 45 patients underwent surgery in the authors' institution. There were 31 femoral and 15 tibial lengthenings. The operation and lengthening procedure were performed according to the rules published by the method's author. The intended amount of lengthening was achieved in 80 p.c. of patients during tibial elongation and in only 52 p.c. during femoral elongation because of many problems, obstacles, and complications during treatment. A significant percentage of improper (deficient or irregular) new bone formation led to femoral malunin in three patients and to tibial and femoral delayed bone consolidation in six patients. Fast distraction produced great forces on the surrounding joint surfaces, resulting in a limited range of motion and joint stiffness. The Wagner technique does not seem to be effective and is associated with a long duration of treatment and some complications impairing the function of the limb.

1248/2523

Tytuł oryginału: Education, science and quality assurance in radiotherapy in Europe.
Autorzy: Heeren Germaine
Źródło: Rep. Pract. Oncol. Radiother. 2002: 7 (1) s.7-9
Sygnatura GBL: 313,406

Hasła klasyfikacyjne GBL:

radiologia

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

1249/2523

Tytuł oryginału: Assessment of early toxicity of concomitant radio-chemotherapy in the treatment of locally advanced cervical cancer.
Autorzy: Wieczorek Andrzej, Kędzierawski Piotr, Smok-Kalwat Jolanta, Banatkiewicz Paweł
Źródło: Rep. Pract. Oncol. Radiother. 2002: 7 (1) s.11-14, il., bibliogr. 10 poz. - Konferencja pt. Postępy w radioterapii onkologicznej - diagnostyka i leczenie, planowanie klinicznego rokowania Poznań 19-21.04. 2001
Sygnatura GBL: 313,406

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Introduction: The aim of this paper was to assess early toxicity and feasibility of concomitant radiochemotherapy in the treatment of locally advanced cervical cancer in daily routine clinical practise. Material and methods: Between 1st of May 2000 and 15th of November 2000, twenty one patients suffering from stage IIB and IIIB (FIGO classification) cervical carcinoma were treated in the Radiotherapy Department of the Holycross Cancer Centre by concomitant radio-chemotherapy. Early toxicity of the skin, the lower gastrointestinal system and the genitourinary system was assessed according to acute radiation morbidity scoring criteria EORTC/RTOG. Leukopenia, thrombocytopenia, anemia, nausea, vomiting and nephrotoxicity were scored according to the WHO scale. Results: There were no treatment-related deaths and no treatment breaks in radiotherapy due to acute toxicity. Only 48 p.c. of patients completed all 6 courses of cis-platin. The main causes of withdrawals from consecutive cycles of chemotherapy were: leucopenia - grade 2 or 3, thrombocytopenia - grade 3 and patient refusal. One patient developed acute renal failure. Other adverse effects did not exceed grade 2. Conclusions: Concomitant radio-chemotherapy in the treatment of locally advanced cervical cancer was feasible and featured acceptable toxicity. Haematologic adverse effects were the main reasons of discontinued chemotherapy. Radiotherapy was well tolerated and all patients completed the whole course.

1250/2523

Tytuł oryginału: Postmastectomy radiotherapy.
Autorzy: Korzeniowski Stanisław
Źródło: Rep. Pract. Oncol. Radiother. 2002: 7 (1) s.15-25, tab., bibliogr. 42 poz. - Konferencja pt. Postępy w radioterapii onkologicznej - diagnoza i leczenie, planowanie klinicznego rokowania Poznań 19-21.04. 2001
Sygnatura GBL: 313,406

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

1251/2523

Tytuł oryginału: The effect of altered fractionation schedule on the spinal cord response: radiobiological considerations.
Autorzy: Supe Sanjay S., Ganesh K. M., Velmurugan J., Rana B. S., Sankar B. N.
Źródło: Rep. Pract. Oncol. Radiother. 2002: 7 (1) s.27-35, tab., bibliogr. 18 poz.
Sygnatura GBL: 313,406

Hasła klasyfikacyjne GBL:

neurologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: Increased fractionation spares late reacting normal tissued more than acute reacting normal tissues. A linear quadratic model is valid from large dose per fraction down to dose per fraction of 2 Gy. Experimental studies on animals and clinical studies on the spinal cord tolerance have shown incidences of myelopathy at doses lower than 50 Gy. The ŕ/á value of the linear quadratic model have been lower for low doses per fraction, indicating a sparing effect of altered fractionation for spinal cord myelitis. Animal data, clinical and radiobiological xplanations suggest of the radiobiological models. Further data suggest that one must not assume the spinal cord to have a greater tolerance at doses per fraction below the conventional dose per fraction of 2 Gy.

1252/2523

Tytuł oryginału: Tumors and the danger model.
Autorzy: Kowalczyk Dariusz W.
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.295-302, il., bibliogr. [26] poz. - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: This article reviews the evidence for the danger model inthe context of immune response to tumors and the insufficiency of the immune system to eliminate tumor growth. Despite their potential immunogenicity tumors do not induce significant immune responses which could destroy malignant cells. According to the danger model, the immune surveillance system fails to detecttumor antigens because transformed cells do not send any danger signals which could activate dendritic cells and initiate an immune response. Instead, tumor cells or antigen presenting cells turn of the responding T cells and induce tolerance. The studies reviewed herein based on model tumor antigens, recombinant viral vectors and detection of tumor specific T cells by MHC/peptide tetramers underscore the critical role of tumor antigen presentation and the context in which it occurs. They indicate that antigen presentation only by activated but not by caner or resting dendritic cells is necessary for the induction of immune responses to tumor antigens. It becomes apparent that the inability of dendiritic cells to become activated provides a biological niche for tumor escape from immune destruction and seems to be a principal mechanisms for the failure of tumor immune surveillance.

1253/2523

Tytuł oryginału: Sialyl Lewis-a: a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells.
Autorzy: Ugorski Maciej, Laskowska Anna
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.303-311, il., bibliogr. s. 308-311 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

tytuł obcojęzyczny

Streszczenie angielskie: Neoplastic transformation is often associated with characteristic changes in the expressionof the sialyl Lewis**a and sialyl Lewis**x antigens, representing typical turmor-associated carbohydrate antigens. High amounts of sialyl Lewis**a are present in human adenocarcinomas of the colon, pancreas and stomach. A growing amount of data suggests that this carbohydrate structure is theligand for E-selectin. Sialylated Lewis structures present on the surface of tumor cells are carried by the carbohydrate chains of glycoproteins and glycolipids. There are several lines of evidence showing that sialyl Lewis**a is responsible for the adhesion of human cancer cells to endothelium. E-selectin present on endothelial cells mediates these interactions. Selectins and their carbohydrate ligands can thus play an important role in the selective homing of tumor cells during metastasis. However, the presence of sialyl Lewis**a antigen on the surface of tumor cells and their adhesion to E-selectin-expressing cells in in vitro adhesion assay by itself can not be directly related to metastatic properties of all cancer cells.

1254/2523

Tytuł oryginału: Antiangiogenic gene therapy in inhibition of metastasis.
Autorzy: Szala Stanisław, Szary Jarosław, Cichoń Tomasz, Sochanik Aleksander
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.313-321, il., tab., bibliogr. s. 319-321 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: This short review attempts to demonstrate the usefulness of antiangiogenic gene therapy in achieving inhibition of growth in experimentally-induced metastases. Certain normal tissues (for example skeletal muscle) may be used in vivo, after genetic modification, as a "bioreactor", able to produce and secrete into the bloodstream rpoteins known to exert antiangiogenic effects. By inhibiting neoangiogenesis these proteins would thus prevent the development of metastases. The review discusses also the perspectives of antimetastatic therapy based on certain types of allogenic cells (for example myoblasts and fibroblasts) that had been genetically modified and then microencapsulated. The strategy of encapsulation is aimed at protecting the modified cells secreting antiangiogenic factors from being eliminated by the immune system. Secretion antiangiogenic factors from being eliminated by the immune system. Secretion of antiangiogenic proteins by these microencapsulated cells can be controlled with inducible promoters. Antiangiogenic genes remaining under the transcriptional control of such promoters may be switched on and off using antibiotics, such as tetracycline derivatives, or steroid hormones.

1255/2523

Tytuł oryginału: Metabolic acitvation of adriamycin by NADPH-cytochrome P450 reductase; overview of its biological and biochemical effects.
Autorzy: Bartoszek Agnieszka
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.323-331, il., tab., bibliogr. 330-331 - 8 Międzynarodowe Sympozjum nt. molekularnych aspektów chemioterapii Gdańsk 05-07.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca związana ze zjazdem

Streszczenie angielskie: NADPH-cytochrome P450 reductase (P450 reductase) is one of the enzymes implicated in the metabolism of adriamycin, a very important clinically used antitumour drug. However, apart from the enzyme involvement, so far little was known about the chemical route and biochemical effects of this process. We demonstrated that the application of P450 reductase simultaneously with adriamycin to tumour cells in culture significantly increased cytotoxicity of the drug. Under tissue culture conditions, we noticed also that, in the presence of P450 reductase, adriamycin metabolite(s), displaying an altered spectrum within the visible light range were formed. This observation was taken adavantage of to study the metabolism of adriamycin in cell-free systems, using initially the enzyme isolated from rat liver and the recently obtained recombinant human P450 reductase. The reductive conversion of the drug turned out to be a multi-stage process, which occurred only under aerobic conditions and was accompanied by excessive NADPH consumption. Further research carried out iwht the aid of radical scavengers and rafdiolabelled adriamycin revealed that the enhancement of biological acitivity of adriamycin by P450 reductase stemmed from the formation of alkylating metabolite(s) rather than from the promotion of redox cycling known to be nduced in the presence of anthracyclines.

1256/2523

Tytuł oryginału: Expression of PiT1 and PiT2 retroviral receptors and transduction efficiency of tumor cells.
Autorzy: Grabarczyk Piotr, Wysocki Piotr J., Gryska Katarzyna, Mackiewicz Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.333-339, il., bibliogr. [10] poz. - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Recombinant retroviral vectors are still the most common gene delivery vehicles for gene therapy purposes, especially for construction of genetically modified tumor vaccines (GMTV). However, theses vehicles are characterized by relatively low titre and in the case of many tumor cell lines, low transduction efficiency. We constructed bicistronic retroviral vector pseudotypes of amphotropic murine leukemia virus (A-MuLV) and gibbon ape elukemia virus (GaLV), encoding enhanced green fluorescent protein (EGFP) as a rapid and easily detectable reporter gene. Transduction of five different human melanoma and four renal carcinoma cell lines by these two virus pseudotypes revealed differences in transduction efficiency, which wase markedly lower for the renal carcinoma cell lines. Stimulation of retroviral receptor expression (PiT1 and PiT2) by phosphate depletion induced a limited increase of receptor mRNA levels, but did not improve the gene transfer efficiency. In contrast, simultaneous transduction with both vector psuedotypes markedly increased the transduction efficiency, compared to GaLV or A-MuLV alone. The same effect could be achieved by several repeated exposures of target cells to fresh vector preparation. Overexpression of GaLV receptor (PiTq) in target cells significantly increased the transduction rate and enabled retrovirus mediated gene transfer into the cells which normally are not transducible by GaLV pseudotypes. We demonstrated that, using different transduction strategies, the relatively inefficient, widely used retroviral vector systems could be significantly improved.

1257/2523

Tytuł oryginału: Abnormal FHIT gene transcript and c-myc and c-erbB2 amplification in breast cancer.
Autorzy: Kowara Renata, Gołębiowski Filip, Chrzan Paweł, Skokowski Jarosław, Karmolinski Andrzej, Pawełczyk Tadeusz
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.341-350, il., tab., bibliogr. [32] poz. - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca związana ze zjazdem

Wskaźnik treści:

ludzie

Streszczenie angielskie: Searching for ways to improve the characterization of breast cancer we examined the realtionship between the status of teh FHIT gene transcript and amplification of c-myc and the c-erbB2 oncogene. Abnormal FHIT transcript was detected in 32 of 79 cancers examined. The presence of Fhit protein estimated by Western blots was evident only in cancers exhibiting a normal-sized FHIT transcript. This indicates that abnormal FHIT transcripts observed in our study did not encode any Fhit protein or the amount of such protien was very low. There was no association between the presence of aberrant FHIT gene transcirpt with age, tumor size, estrogen and progesterone receptor status, local metastases and histological grading. However, the abnormalities in FHIT gene transcripts were observed with different frequency depending on the histopathological type of the tumor. The aberrant FHIT transcript was detected in 60 p.c. of lobular cancers and only in 28 p.c. of ductal cancers. Analyzing the occurrence of c-myc and c-erbB2 amplification and the presence of aberrant FHIT gene transcripts we found that the aberrant FHIT transcirpt more frequently occurred in tissues with c-myc amplification. There was a significant (P 0.05) correlation between the occurrence of the aberrant FHIT gene transcript with accompanying c-mcy amplification and positive lymph node status. However, in order to evaluate the predictive value of these findings in breast cancer, an extended clincal follow up will be necessary.

1258/2523

Tytuł oryginału: Germline mutations in the BRCA1 gene predisposing to breast and ovarian cancers in Upper Silesia population.
Autorzy: Grzybowska Ewa, Siemińska Marzena, Zientek Helena, Kalinowska Ewa, Michalska Jadwiga, Utracka-Hutka Beata, Rogozińska-Szczepka Jadwiga, Kaźmierczak-Maciejewska Maria
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.351-356, tab., bibliogr. s. 354-356 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca epidemiologiczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Germline mutations in the BRCA1 or BRCA2 genes predispose their cariers to breast or/and ovary cancers during their lifetime. The most frequent mutations: 5382insC, 185delAG, C61G and 4153delA in BRCA1, and 6174delT and 9631delC in BRCA2 were studied in a group of 148 probands admitted for genetic counseling, using allele-specific amplification (ASA) PCR test. Fifteen carriers of three different mutations: 5382insC, 185delAG and C61G in BRCA1 were found. Two families carried the 185delAG mutationand additional two C61G in BRCA1. Nobody carried the mutation 4153delA in BRCA1 nor 617delT or 9631delC in BRCA2. Most of the carriers of a germline mutation were observed among the patients who developed bilateral breast cancer (17 p.c.). The lowest frequency of the germline mutations was found inthe healthy persons who had two or more relatives affected with breast or ovarian cancer.

1259/2523

Tytuł oryginału: Comparative analysis of prostatic acid phosphatase and prostat-specific antigen mRNA levels in hyperplastic prostate stimulated with steroid hormones and growth factors.
Autorzy: Dulińska Joanna, Laidler Piotr, Łabędź Maria
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.357-368, il., tab., bibliogr. s. 366-368 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) are the markers of human prostatic gland. However, it is still not completely understood if and how, steroid hormones and growth factors affect their expression and metabolism in the respect to the major pahtologies of the gland. Appropriate studies were carried out on histopathologically diagnosed benign prostatic hyperplasia - BPH (n - 42) using tissue slices and cells derived from them. They were incubated with steroid hormones: 5-ŕ-dihydrotestosterone (DHT), estradiol (E) and growth factors: epidermal growth factor (EGF), basic fibroblastic growth factor (bFGF) under culture conditiions for up to 24 hours. 32P-labelled specific oligonucleotide probes were used to analyze total RNA isolated from each sample for the presence of PAP and PSA mRNAs. DHT, E, bFGF, EGF or both DHT + bFGF and DHT + EGF increased PAP and PSA mRNA levels in a time- and dose-dependent manner. The highest and statistically significant increase (p 0.001) for PAP mRNA was observed when DHT + bFGF were present in the medium while for PSA mRNA if DHT + EGF were added to the medium.

1260/2523

Tytuł oryginału: Analysis of mutations in the p16/CDKN2A gene in sporadic and familial melanoma in the Polish population.
Autorzy: Lamperska Katarzyna, Karczewska Aldona, Kwiatkowska Eliza
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.369-376, il., tab., bibliogr. s. 374-376 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca epidemiologiczna

Streszczenie angielskie: Mutations in CDKN2A have been found in sporadic cutaneous malignant (CMM), in familial CMM and in other syndromes associated with melanoma. In this study DNA was obtained from 207 individuals and five cell lines. There were 157 CMM patients and 50 healthy members of melanoma patients families. The CMM group included patients with one or two melanoma cases in the family, families with dysplastic nevus syndrom (DNS) and patients with a spectrum of other types of cancers in the family. PCR-SSCP analysis and sequencing identified: six substitutions in codon 58 CGA/TGA (Arg/Stop), 16 substitutions GAC/GAT in codon 84 (Asp/Asp), six substitutions CGA/TGA in codon 148 (Arg/Thr), 14 substitutions G/C in 3'UTR and 4 double changes (two in codon 84 and 3'UTR; two in codon 148 and 3'UTR). THe mutation identified in codon 58 was found in tissue only. Other substitutions were polymorphisms found in DNA from tissue and blood samples. Most of them were identified in sporadic CMM (six in codon 148 Ala/Thr, 12 in codon 84 Asp/Asp and six in 3'UTR). The frequency of the polymorphisms was also high in DNS and CMM/DNS families (four in codon 84 Asp/Asp and six in 3'UTR). No mutations or polymorphisms were found in CMM patients with one or two melanoma cases and CMM patients, with other cancers in family history. The analysis of the CDKN2A gene muataions in the Polish population demonstrated: (i) no germline mutations; (ii) a relatively high number of genetic changes in sporadic melanoma; (iii) a high number of polymorphisms in DNS and CMM/DNS families.

1261/2523

Tytuł oryginału: Expression of p16 in sporadic primary uveal melanoma.
Autorzy: Lamperska Katarzyna, Mackiewicz Krystyna, Kaczmarek Aldona, Kwiatkowska Eliza, Starzycka Maria, Romanowska Bożena, Heizman Janina, Stachura Jerzy, Mackiewicz Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.377-385, il., tab., bibliogr. s. 383-385 - 37 Spotkanie Polskiego Towarzystwa Biochemicznego Toruń 10-14.09. 2001
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

okulistyka

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

dorośli = 65 r.ż.

Streszczenie angielskie: Expression of p16 protein, intragenic mutations of CDKN1A and hypermethylation of CDKN2A promoter region in 41 sporadic primary uveal melanomas were studied. There were 2 cases of spindle cell B histological type, 11 of A + B and 28 of mixed type. All melanomas infiltrated sclera but in 28 cases infiltration was superficial while in 13 profound. In 7 cases the tumor infiltrated the optic nerve. Expression of p16 was studied by immunohistochemistry and recorded by assessment of the proportion of positive tumor cells and staining intensity. Results were expressed as staining index (RS). Intragenic mutations were studied by PCR-SSCP followed by sequencing, while hypermethylation of the promoter region by CpG methylation assay. In 15 p.c. of cases less than 10 p.c. of melanoma cells were p16 positive, in 70 p.c. of cases less than 50 p.c. of cells, while in 7 p.c. more than 80 p.c. of cells stained for p16 (mean IRS for all cases was 4.87 ń 2.43). In B type the IRS was 8.5 ń 0.7, in A + B type 6.0 ń 2.1 and in the mixed type 4.17 ń 2.43). In B type the IRS was 8.5 ń 0.7, in A + B type 6.0 ń 2.1 and in the mixed type 4.17 ń 2.43 (differences statistically significant). In melanomas profoundly infiltrating sclera mean (IRS was 4.16, while in those infiltrating optic nerve 3.71 (statistically not significant). Analysis of the intragenic mutations revealed in two patients a GAC/GAT substitution in codon 84 - a silent mutation. No hypermethylation of the CpG island of the p16 promoter region was found. In conclusion, we found that the degree of p16 expression is related to the histological type of tumor but not to the histological indicators of tumor invasiveness and that intragenic mutations and promoter hypermethylation are not major mechanisms of p16 inactivation in sporadic uveal melanoma.

1262/2523

Tytuł oryginału: Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.
Autorzy: Urbańska Krystyna, Romanowska-Dixon Bożena, Matuszak Zenon, Oszajca Janusz, Nowak-Śliwińska Patrycja, Stochel Grażyna
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.387-391, il., bibliogr. [9] poz. - 5 Sympozjum nt. wolnych rodników w biologii i medycynie Łódź 07-10.06. 2000
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect of photodynamic therapy (PDT) were evaluated by following the growth of human (SKMEL 188) and mouse (S91) melanoma cells. The surviving fraction of the cells irradiated (lambda ex = 830nm) vs non-irradiated, treated with the same dose of ICG, is significantly decreased (5- to 10-fold). These results show that ICG is a very promising dye for photodynamic therapy of melanomas.

1263/2523

Tytuł oryginału: Side-chain modified vitamin D analogs require activation of both PI 3-K and erk 1,2 signal transduction pathways to induce differentiation of human promyelocytic leukemia cells.
Autorzy: Marcinkowska Ewa, Kutner Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.393-406, il., tab., bibliogr. s. 404-406
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

hematologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: Synthetic analogs of vitamin D for potential use in differentiation therapy should selectively regulate genes necessary for differentiation without inducing any perturbations in calcium homeostasis. PRI-1906, an analog of vitamin D2, and PRI-2191, an analog of vitamin D3 bind nuclear vitamin D receptor (nVDR) with substantially lower affinity than 1,25-dihydroxyvitamin D3 (1,25-D3), but have higher differentiation-inducing activity as estimated in HL-60 leukemia cell model. To examine how their increased differentiation-inducing activity is regulated we tested the hypothesis that membrane-mediated events, unrelated to nVDR, take part in the differentiation in response to PRI-1906 and PRI-2191. The induction of leukemia cell differentiation in response to the analogs of vitamin D was inhibited by LY294002 (phosphatidylinositol 3-kinase inhibitor), PD 98059 (inhibitor of MEK1,2, an upstream regulator of extracellular-signal regulated kinase) and rapamycin (p70S6K inhibitor) pointing out that activation of signal transduction pathways unrelated to nVDR is necessary for differentiation. On the other hand, inhibition of cytosoloic phospholipase A2 accelerated the differentiation of HL-60 cells induced by either 1,25-D3 or by the vitamin D analogs suggesting possible existence of a feedback loop between extracellular-signal regulated kinases and phospholipase A2.

1264/2523

Tytuł oryginału: Sulfamide antifolates inhibiting thymidylate synthase: synthesis, enzyme inhibition and cytotoxicity.
Autorzy: Pawełczak Krzysztof, Makowski Maciej, Kempny Michał, Dzik Jolanta M., Gołos Barbara, Rode Wojciech, Rzeszotarska Barbara
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.407-420, il., tab., bibliogr. s. 418-420 - 1046 Międzynarodowe Sympozjum nt. biologii i chemii pochodnych pterydyny i folionówMiędzynarodowe Sympozjum nt. molekularnych aspektów chemioterapiiMiędzynarodowy Kongres nt. leczenia przeciwnowotworowego Orange BeachGdańskParyż 199319931996
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

onkologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Synthesis and biological evaluation are described of seven new analogues (309) of two potent thymidylate synthase inhibitors, 10-propargyl-5,8-dideazafolate (1) and its 2-methyll-2-deamino congener ICI 198583 (2). While the new compounds 3 and 4 were analogues of 1 and 2, respectively, containing a p-aminobenzenesulfonyl residue in place of the p-aminobenzoic acid residue, the remaining 5 new compounds were analogues of 4 with the L-glutamic acid residue replaced by glycine (5), L-valine (6), L-alanine (7), L-phenylglycine (8) or L-norvaline (9). The new analogues were tested as inhibitors of thymidylate synthases isolated from tumour (Ehrilich carcinoma), parasite (Hymenolepis diminuta) and normal tissue (regenerating ratliver) and found to ve weaker inhibitors than the parent 10-propargyl-5,8-dideazafolic acid. Selected new analogues, tested as inihbitors of growth of mouse leukemia L 5178Y cells, were less potent than the parent 10-propargyl-5,8-dideazafolic acid. Substitution of the glutamyl residue in compound 4 with L-norvaline (9) resulted in only a 5-fold stronger thymidylate synthase inhibitor, but a 40-fold weaker cell growth inhibitor.

1265/2523

Tytuł oryginału: Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesorcricetus auratus) with the use of universal primers.
Autorzy: Dobrzańska Zuzanna, Więckiewicz Joanna, Bigda Jacek
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.427-431, il., bibliogr. [15] poz. - 6 Spotkanie Młodych Immunologów Sekcji Polskiego Towarzystwa Immunologii Doświadczalnej i Klinicznej Gdańsk 2000
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

immunologia

Typ dokumentu:

komunikat

Wskaźnik treści:

chomiki

płeć męska

Streszczenie angielskie: In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.

1266/2523

Tytuł oryginału: Mutations in type I collagen genes resulting in osteogenesis imperfecta in humans.
Autorzy: Gajko-Galicka Anna
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.433-441, il., tab., bibliogr. s. 439-441
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

traumatologia i ortopedia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Osteogeneis imperfecta (OI), commonly known as "brittle bone disease", is a dominant autosomal disorder characetrized by bone fragility and abnormalities of connective tissue. Biochemical and molecular genetic studies have shown that the vast majority of affected individuals have mutations in either the COL1A1 or COL1A2 genes that encode the chains of type I procollagen. OI is associated with a wide spectrum of phenotypes varying from mild to severe and lethal conditions. The mild forms are usually caused by mutations which inactivate one allele of COL1A1 gene and result in a reduced amount of normal type I collagen, while the severe and lethal forms result from dominant negative mutations in COL1A1 or COL1A2 which produce structural defects in the collagen molecule. The most common mutations are substitutions of glycine residues, which are crucial to formation and function of the collagen triple helix, by larger amino acids. Although type I collagen is the major structural protein of both bone and skin, the mutations in type I collagen genes cause a bone disease. Some reports showed that the mutant collagen can be expressed differently in bone and in skin. Since most mutations identified in OI are dominant negative, the gene therapy requires a fundamentally different approach from that used for genetic-recessive disorders. The antisense therapy, by reducing the expression of mutant genes, is able to change a structural mutation into a null mutation, and thus convert severe forms of the disease into mild OI type I.

1267/2523

Tytuł oryginału: A novel Gly to Arg substitution at position 388 of the ŕ1 chain of type I collagen in lethal form of osteogenesis imperfecta.
Autorzy: [Gajko]-Galicka Anna, Wołczyński Sławomir, Leśniewicz Ryszard, Chyczewski Lech, Gindzieński Andrzej
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.443-450, il., bibliogr. s. 449-450
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

praca epidemiologiczna

Wskaźnik treści:

ludzie

Streszczenie angielskie: Cultured skin fibroblasts from a proband with a lethal form of osteogenesis imperfecta produce two forms of type I collagen chains, with normal and delayed electrophoretic migration; collagen of the proband's motehr was normal. Peptide mapping experiments localized the structural defect in the proband to ŕ1(I) CB8 peptide in which residues 123 to 402 are spaned. Direct sequencing of amplified cDNA covering this region revealed a G to A single base change in one allele of the ŕ1(I) chain, that converted glycine 388 to arginine. Restriction enzyme digestion of the RT-PCR product was consistent with a heterozygous COL1A1 mutation. The novel mutation conforms to the linear gradient of clinical severity for the ŕ1(I) chain and results in reduced thermal stability by 3řC and intracellular retention of abnormal molecules.

1268/2523

Tytuł oryginału: Collagen of umbilical cord vein and its alterations in pre-eclampsia.
Autorzy: Romanowicz Lech, Jaworski Stefan
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.451-458, il., bibliogr. s. 456-458
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

ginekologia i położnictwo

Typ dokumentu:

praca kliniczna

Wskaźnik treści:

ciąża

Streszczenie angielskie: The state of the vascular system of the mother and of placenta is known to exert a great influence on intrauterinal development of the fetus. Pre-eclampsia is the most common pathological syndrome connected with pregnancy. Since collagen is one of the main constituents of the vessel wall a comparison was made with collagen content and its molecular polymorphism in umbilical cord veins of newborns from healthy and pre-eclamptic mothers. It was found that umbilical cord veins of newborns from mothers with pre-eclampsia contained 18 p.c. less collagen than those of the newborns from normal pregnancies. This decrease was accompanied by a sligh decrease of collagen solubility, but all its types (I, III, IV, V and VI) were present. However, the umbilical vein wall of newborns from mothers with pre-eclampsia contained relatively less of type I and more of type III collagen than the normal umbilical cord. These differences may be connected with a disturbance of blood flow in fetus of a woman with pre-eclampsia.

1269/2523

Tytuł oryginału: Insight into the kinetics and the mode of the interaction between smooth muscle calponin and F-actin.
Autorzy: Kołakowski Janusz, Dąbrowska Renata
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.471-479, il., tab., bibliogr. s. 478-479
Sygnatura GBL: 303,116

Typ dokumentu:

praca doświadczalna

Streszczenie angielskie: Kinetics of the smooth muscle calponin-F-actin interaction was studied by stopped-flow measurements of light scattering and fluorescence intensity of pyrene-labelled F-actin. The intensity and character of the changes in light scattering, and thus the mode of calponin binding to actin filaments leading to changes in their shape and bundling, depend on the molar ration of the two proteins. Parallel measurements of pyrene-fluorescence quenching upon calponin binding revealed that intrinsic conformational changes in actin filaments are delayed relative to the binding process and are not markedly influenced by the mode of calponin binding. Bundling of actin filaments by calponin was not correlated with fluorescence changes and thus with alterations in the structure of actin filaments.

1270/2523

Tytuł oryginału: Interaction of glycophorin A with lectins as measured by surface plasmon resonance (SPR).
Autorzy: Krotkiewska Bożena, Pasek Marta, Krotkiewski Hubert
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.481-490, il., tab., bibliogr. s. 489-490
Sygnatura GBL: 303,116

Typ dokumentu:

praca doświadczalna

Streszczenie angielskie: Glycophorin A (GPA), the major sialoglycoprotien of the human erythrocyte membrane, was isolated from erythrocytes of healthy individuals of blood groups A, B and O using phenol-water extraction of erythrocyte membranes. interaction of individual GPA samples with three lectins (Psathyrella velutina lectin, PVL; Triticum vulgaris lectin, WGA and Sambucus nigra I agglutinin SNA-I) was analyzed using a BIAcore**TM biosensor equipped with a surface plasmon resonance (SPR) detector. The experiments showed no substantial differences in the interaction between native and desialylated GPA samples originating from erythrocytes of either blood group and each of the lectins. Desialylated samples reacted weaker than the native ones with all three lectins. PVL reacted about 50-fold more srongly than wGA which, similar to PVL, recognized GlcNAc and Neu5Ac residues. SNA-I lectin, recognizing ŕ2-6 linked Neu5Ac residues, showed relatively weak reaction with native and only residual reaction with desialylated GPA samples. The data obtained show that SPR is a valuable method to determine interaction of glycoproteins with lectins, which potentially can be used to detect differences in the carbohydrate moiety of individual glycoprotein samples.

1271/2523

Tytuł oryginału: The structure of the oligosaccharides of ŕ3á1 integrin from human ureter epithelium (HCV29) cell line.
Autorzy: Lityńska Anna, Pocheć Ewa, Hoja-Łukowicz Dorota, Kremser Elżbieta, Laidler Piotr, Amoresano Angela, Monti Chiara
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.491-500, il., tab., bibliogr. s. 499-500
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

urologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

in vitro

Streszczenie angielskie: There is a growing line of evidence that glycosylation of ŕ and á subunits is important for the function of integrins. Integrin ŕ3á1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin ŕ3á1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi-tri- and tetraantennary structures were the most common, while hig-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while tha predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.

1272/2523

Tytuł oryginału: The ç-glutamyltransferase activity and non-protein sulfhydryl compounds levels in rat kidney of different age groups.
Autorzy: Włodek Przemysław, Sokołowska Maria, Smoleński Olgierd, Włodek Lidia
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.501-507, il., bibliogr. s. 506-507
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The present work was aimed to obtain information about age-dependent changes of ç-glutamyltransferase (GGT) activity and the levels of non-protein sulfhydryl compounds (NPSH) in rat kidneys. In addition, protein-bound cysteine (PB-Cys), sulfane sulfur compounds and reactive oxygen species (ROS) were estimated. The results indicate that the activity of GGT and NPSH levels in the kidneys are reduced with age. At the same time, a significant increase in the level of protein-bound cysteine was observed. Simultaneously, the content of sulfane sulfur compounds was increased in the group of the oldest animals. These findings indicate that the capacity for extracellular glutathione degradation adn, in consequence, the availability of cysteine for intracellular glutathione biosynthesis may be impaired. The increased PB-Cys level indicates potentiation of the thiolation reaction, i.e. development of protein-mixed disulfides. These results reveal age dependent disturbances in the thiol-disulfide equilibrium in the dineys which leads to an imbalance between pro- and antioxidatory processes.

1273/2523

Tytuł oryginału: Spectrophotometric method for the determination of renal ouabain-sensitive H+, K+ -ATPase activity.
Autorzy: Bełtowski Jerzy, Wójcicka Grażyna
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.515-527, il., tab., bibliogr. s. 526-527
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: The aim of this work was to develop a method for renal H+, K+ -ATPase measurement based on the previously used Na+, K+ -ATPase assay (Bełtowski et al.: J Physiol Pharmacol.; 1998, 49: 625-37). ATPase activity was assessed by measuring the amount of inorganic phosphate liberated from ATP by isolated microsomal fraction. Both ouabain-sensitive and ouabain-reistant K+ - stimulated and Na+ -independent ATPase activity was detected in the renal cortex and medulla. These activities were blocked by 0.2 mM imidazolpyridine derivative, Sch 28080. The method for ouabainsensitive H+, K+ -ATPase assay is characterized by good reproducibility, linearity and recovery. In contrast, the assay for ouabain-resistant H+, K+ -ATPase was unsatisfactory, probably due to low activity of this enzyme. Ouabain-sensitive H+, K+ -ATPase was stimulated by K+ with Km of 0.26 ń 0.04 mM and 0.69 ń 0.11 mM in cortex and medulla, respectively, and was inhibited by ouabain (Ki of 2.9 ń 0.3 ćM in the renal cortex and 1.9 ń 0.4 ćM in the renal medulla) and by Sch 28080 (Ki of 1.8 ń 0.5 ćM and 2.5 ń 0.9 ćM in cortex and medulla, respectively). We found that ouabain-sensitive H+, K_ -ATPase accounted for about 12 p.c. of total ouabain-sensitive activity in the Na+, K+ -ATPase assay. Therefore, we suggest to use Sch 28080 during Na+, K+ -ATPase measurement to block H+, K+ -ATPase and improve the assay specificity. leptin administered intraperitoneally (1 mg/kg) decreased renal medullary Na+, K+ -ATPase activity by 32.1 p.c. at 1 h after ijection but had no effect on H+, K+ -ATPase activity suggesting that the two renal ouabain-sensitive ATPases are separately regulated.

1274/2523

Tytuł oryginału: Changes in antioxidant status of heart muscle tissue in experimental diabetes in rabbits.
Autorzy: Gumieniczek Anna, Hopkała Hanna, Wójtowicz Zbigniew, Nikołajuk Justyna
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.529-535, tab., bibliogr. s. 534-535
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

kardiologia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

zwierzęta

Streszczenie angielskie: The present study was designed to evaluate the oxidative stress-related parameters in alloxan-induced diabetes in rabbits. After 3, 6, 12 and 24 weeks of hyperglycaemia the enzymatic and non-enzymatic factors were measured in heart tissue fo diabetic and control groups. Superoxide dismutase and glutathione peroxidase activities and the contents of total sulfhydryl compounds signficantly increased at all time intervals. Catalase activity increased initially (after 3 and 6 weeks), decreased after 12 weeks and increased again at the 24th week of the experiment. Glutathione reductase activity increased initially (at 3rd week), decreased below control level after 6 and 12 weeks, then increased again. Ascorbic acid concentration decreased after 3 and 6 weeks, and increased at the 12th and 24th weeks. The level of lipid peroxidation products was reduced after 3, 6 and 12 weeks of the experiment. After 24 weeks it was significantly elevated. These data suggest that hyperglycaemia induces oxidative stress in the heart but the defense mechanisms in the heart tissue are fairly efficacious against oxidative injury.

1275/2523

Tytuł oryginału: 10th anniversary of the UNESCO/Polish Academy of Sciences Cellular and Molecular Biology Network.
Autorzy: Rędowicz M. Jolanta
Źródło: Acta Bioch. Pol. 2002: 49 (2) s.547-549
Sygnatura GBL: 303,116

Hasła klasyfikacyjne GBL:

genetyka

Typ dokumentu:

informator

1276/2523

Tytuł oryginału: Upregulation of fatty acid synthase gene expression in experimental chronic renal failure.
Autorzy: Szołkiewicz Marek, Niewęgłowski Tomasz, Korczyńska Justyna, Sucajtys Elżbieta, Stelmańska Ewa, Goyke Elżbieta, Świerczyński Julian, Rutkowski Bolesław
Źródło: Metabolism 2002: 51 (12) s.1605-1610, il., tab., bibliogr. 36 poz.
Sygnatura GBL: 303,583

Hasła klasyfikacyjne GBL:

nefrologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć męska

Streszczenie angielskie: Hypertriglyceridemia associated with chronic renal failure (CRF) and elevated plasma concentration of very-low-density lipoprotein (VLDL) are thought to be a consequence of the depressed lipoprotein lipase and hepatic lipase activities and impaired clearance of lipoproteins. However, ther is some evidence that the lipoproteins overproduction might also contribute to hypertriglyceridemia in CRF. This study was performed to test the hypothesis that the increased rate of lipogenesis consequent to upregulation of fatty acid synthase (FAS), a key lipogenic enzyme, gene expression could contribute to overproduction of triacylglycerols and to hypertriglyceridemia in CRF. FAS activity, FAS protein mass (Western nlot analysis), and FAS mRNA level (Northern blot analysis) in liver and epididymal white adipose tissue (WAT) were measured in male Wistar rats 6 weeks after subtotal (5 of 6) nephrectomy or shan operation. Moreover, the rate of lipogenesis in WAT was determined. The CRF group showed significant increase in FAS gene expression (measured as activity, mRNA, and protein abudance) in both liver and WAT. This was associated with the increase in the lipogenesis rate and with the increase in plasma triacylglycerol and VLDL concentrations. Our results suggest that not only decreased removal, but also an increase of triacylglycerol production could contribute, in part, to the CRF-associated hyperlipidemia. Upregulation of FAS gene expression, shown in this report for the first time, reveals another factor involved in disturbed lipid metabolism in CRF....

1277/2523

Tytuł oryginału: Morphological lesions detected by light and electron microscopies in chronic type B hepatitis.
Autorzy: Kasprzak Aldona, Biczysko Wiesława, Adamek Agnieszka, Zabel Maciej
Źródło: Pol. J. Pathol. 2002: 53 (3) s.103-115, il., tab., bibliogr. 90 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

gastroenterologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

ludzie

Streszczenie angielskie: Apart from serologicla daignosis of chronic type B hepatitis, of high importance is specific morphological diagnosis, based on evaluation of liver biopsies. In the evaluation, the techniques are employed, which directly visualize the virus in the cells (electron microscopy) as well as the techniques of cell biology which demonstrate the presence of viral genetic material and viral proteins in situ. This paper reviews the available data on the diagnosis of liver pathomorphology using the above mentioned techniques in chronic HBV infections in adults. The data have been compared with the results of our own studies, performed in children. In chronic type B hepatitis and more frequently in children than in adults, slight or moderate inflammation (grade 1 to 2) and insignificantly advanced fibrosis (stage 1 to 2) are noted in the liver. Both in children and in adults, lesions in hepatocyte nuclei represent the common morphological denominator in the patterns of light and electron microscopy. The cell nuclei are of variable size, irregular shape, they stain irregularly, manifest an altered outline of nuclear envelope, frequently exhibit numerous and enlarged cell nucleoli and chromatin dissociation (the so called empty cell nuclei). In ultrastructural studies, hepatocyte cytoplasm contains Dane's bodies and tubular forms of HBsAg while virus-resembling particles are noted in cell nuclei. Molecular biology techniques (immunocytochemistry, in situ hybiridisation) reveals nuclear and/or cytoplasmic location of HBcAg, cytoplasmic location of HBsAg and a similar location of HBV DNA. THe data permit us to determine precisely the stage of infection and to make appropriate therapeutic decisions.

1278/2523

Tytuł oryginału: Morphological and immunological features of liver inflammatory infiltrate in children with chronic hepatitis C.
Autorzy: Woźniakowska-Gęsicka Teresa, Wiśniewska-Ligier Małgorzata, Kałużyński Andrzej, Turant Maria
Źródło: Pol. J. Pathol. 2002: 53 (3) s.117-122, il., tab., bibliogr. 22 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

pediatria

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: The aim of the study was to assess the correlation between morphological and immunological features of lesions in the liver in children with chronic hepatitis C. In the study 11 children with chronic hepatitis C were enrolled. Histopathological and immunomorphological investigations included antigen marking CD45, CD45RA, CD45RO, CD20, CD3, CD8 in liver biopsy specimens. The immunomorphological assessment of the inflammatory infiltrate cells showed the presence of CD45 antigen on the vast majority of leukocytes in the portal tracts and in the lobular parenchyma in all the studied children. One fourth of the inflammatory cells were characterised by CD20 phenotype, 1/2 - by CD3 phenotype, 1/3 - by CD8phenotype, 1/3 - by CD45RA phenotype and 2/3 - by CD45RO phenotype. Conclusions: T and B lymphocytes were predominant in hepatic inflammatory infiltrates (T lymphocytes are twice as numerous as B lymphocytes). Among T lymphocytes, cytotoxic and suppressor cells were prevailing. Most T lymphocytes were characterized by CD45RO phenotype, which shows their activation. A positive correlation between most lymphoid cell markers, staging and grading suggests the role of these cells in liver injury.

1279/2523

Tytuł oryginału: Expression of vascular endothelial growth factor (VEGF) in human thyroid tumors.
Autorzy: Lewy-Trenda Iwona, Wierzchniewska-Ławska Agnieszka
Źródło: Pol. J. Pathol. 2002: 53 (3) s.129-132, tab., bibliogr. 45 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

endokrynologia

Typ dokumentu:

tytuł obcojęzyczny

Wskaźnik treści:

płeć żeńska

Streszczenie angielskie: Vascular endothelial growth factor (VEGF) is one of the factors related to angiogenesis of tumors and physiological and pathological proliferative processes. Location of VEGF in human thyroid tissue with various thyroid disorders was studied in order to explore its possible involvement in proliferative processes. Immunohistochemical examination was performed on 69 formalin-fixed, paraffin-embedded thyroid tissue specimens using the labelled streptoavidin biotin peroxidase complex detection system. VEGF was not identified in normal thyroid follicular cells. Some but not all tumor thyreocytes expressed VEGF in cytoplasm. VEGF positive expression was found in 6/15 patients with papillary carcinoma, 4/9 with follicular carcinoma, 14/28 with follicular adenoma and 2/17 with adenomatous goiter. In benign follicular adenoma and adenomatous goiter the weak expression of VEGF was found in small areas of the tumor tissues, whereas in malignant tumors it was found strongly in many cells. VEGF probably functions as a hypoxia-inducible angiogenic factor, and the expression of VEGF is stronger in malignant tumors, which need more oxygen supply to proliferate. An intensive VEGF production by differentiated thyroid carcinomas could be a promising marker of tumor aggressiveness and may also be useful as a predictor of metastatic potential and extension of tumor mass.

1280/2523

Tytuł oryginału: The effect of cisplatin, etoposide and quercetin on Hsp72 expression.
Autorzy: Jakubowicz-Gil Joanna, Paduch Roman, Gawron Antoni, Kandefer-Szerszeń Martyna
Źródło: Pol. J. Pathol. 2002: 53 (3) s.133-137, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 301,852

Hasła klasyfikacyjne GBL:

farmacja

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

in vitro

Streszczenie angielskie: Heat shock protein 72 (Hsp72) belongs to a group of proteins referred to as molecular chaperones that protect normal and tumoru cells against many stressors such as hyperthermia, some commonly used chemotherapeutics and other apoptotic stimuli. Our study was designed to determine whether heat shock and drugs like cisplatin, etoposide and quercetin influence the expression of heat shock protein 72 in tumour cells: HeLa (cervical cancer), Hep-2 (larynx cancer), A549 (lung cancer) and normal human skin fibroblasts (HSF). Our results indicated that Hsp72 expression was drug and cell-type specific. Cisplatin and etoposide did not induce Hsp72 expression in any tumour cells but cisplatin alone induced Hsp72 expression in normal human fibroblasts. Quercetin inhibited heat shock protein expression in Hep-2 cells but induced in HSF. In thermally shocked tumour cells, the expression of Hsp72 was not altered by cisplatin and etoposide, but in normal HSF cells enhanced expression in normal and tumour cells.

1281/2523

Tytuł oryginału: M. D. Program Catalogue and Student Guide
Opracowanie edytorskie: Chyczewska Barbara (ed.), Shaw John Geoffrey (ed.), Tandyrak Mirosława (ed.).
Źródło: - Poznań, University Publishing Office Karol Marcinkowski University of Medical Sciences 2002, 87, [1] s. : il., 22 cm.
Sygnatura GBL: 735,681

Typ dokumentu:

informator

Temat korporatywny:

Akademia Medyczna w Poznaniu

1282/2523

Tytuł oryginału: General physics a brief guide
Autorzy: Kubisz Leszek, Tuliszka Marek
Opracowanie edytorskie: Limanowska-Shaw Honorata (przedm.).
Źródło: - Poznań, Dział Wydaw. Uczelnianych AM 2002, 64 s. : il., 24 cm.
Sygnatura GBL: 735,685

Typ dokumentu:

1283/2523

Tytuł oryginału: Origins of biomechanics : factors of regress and progress
Opracowanie edytorskie: Kornecki Stefan (ed.).
Academy of Physical Education in Wrocław, Polish Society of Biomechanics
Źródło: - Wrocław, Wydaw. AWF 2002, 95, [2] s. : il., tab., bibliogr. [przy ref.], 24 cm. - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Seria: Studia i Monografie/AWF we Wrocławiu 63
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

Typ dokumentu:

1284/2523

Tytuł oryginału: Cases in musculoskeletal biomechanics : research, answers to scientific questions and solutions to clinical problems
Autorzy: Perre Georges Van der
Źródło: W: Origins of biomechanics : factors of regress and progress - Wrocław, 2002 s.7-13, bibliogr. s. 12-13 - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

traumatologia i ortopedia

Typ dokumentu:

1285/2523

Tytuł oryginału: Myofsacial force transmission as a factor of regress and progress
Autorzy: Huijing Peter A.
Źródło: W: Origins of biomechanics : factors of regress and progress - Wrocław, 2002 s.15-27, il., bibliogr. s. 24-27 - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

traumatologia i ortopedia

Typ dokumentu:

praca doświadczalna

Wskaźnik treści:

1286/2523

Tytuł oryginału: Milestones of progress in research methods in biomechanics
Autorzy: Morawski Janusz M.
Źródło: W: Origins of biomechanics : factors of regress and progress - Wrocław, 2002 s.29-42, il., bibliogr. s. 41-42 - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

Typ dokumentu:

1287/2523

Tytuł oryginału: An attempt at creating a mathematical and an artificial neural network models of a simple upper extremity movement
Autorzy: Wit Andrzej, Wychowański Michał
Źródło: W: Origins of biomechanics : factors of regress and progress - Wrocław, 2002 s.43-54, il., tab., bibliogr. s. 53-54 - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

Typ dokumentu:

praca przeglądowa

Wskaźnik treści:

1288/2523

Tytuł oryginału: Matching the force requirements in human spine modelling
Autorzy: Acar B. Serpil, Grilli Susannah L.
Źródło: W: Origins of biomechanics : factors of regress and progress - Wrocław, 2002 s.55-61, il., bibliogr. s. 60-61 - 13 Międzynarodowe Seminarium pt. Podstawy biomechaniki - czynniki cofania się i postępu Wrocław 05-07.10. 2001
Sygnatura GBL: 735,614

Hasła klasyfikacyjne GBL:

medycyna sportowa

traumatologia i ortopedia

Typ dokumentu:

praca przeglądowa

Wskaźnik treści: