Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: PRACA OPUBLIKOWANA ZA GRANICĄ
Liczba odnalezionych rekordów: 355



Przejście do opcji zmiany formatu | Wyświetlenie wyników w wersji do druku

1/355

Tytuł oryginału: Antiaggregatory activity of hypoglycaemic sulphonylureas.
Autorzy: Siluk D., Kaliszan R., Haber P., Petrusewicz J., Brzozowski Z., Sut G.
Źródło: Diabetologia 2002: 45 (7) s.1034-1037, il., tab., bibliogr. 11 poz.
Sygnatura GBL: 312,048

Hasła klasyfikacyjne GBL:
  • endokrynologia
  • farmacja

    Typ dokumentu:
  • praca kliniczna
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • in vitro

    Streszczenie angielskie: Aims/hypothesis. Vascular complications observed in diabetes are often related to altered platelet functions. The most widely used hypoglycaemic drugs for treating Type II (non-insulin-dependent) diabetes mellitus are sulphonylurea derivatives. The purposes of this study were to evaluate the inhibitory effects of hypoglycaemic agents on platelet aggregation, to measure their lipophilicity and identify their structural parameters which assess their antiaggregatory activity. Methods. An antiaggregatory test in vitro was carried out for 13 sulphonylurea derivatives. Aggregation of platelets, incubated with the agents at concentrations varying from 7.5 to 480 ćmol/l, was induced by 10 ćmol/l ADP. Drug lipophilicity parameter, log kw, was measured by gradient HPLC and the agents were subjected to molecular modelling. Results. The most pronounced inhibition of platelet aggregation was by glimepiride, gliclazide, gliquidone, glibenclamide and compound 2A. The IC25 values were 15.9, 18.6, 20.4, 28.5 and 34.7 ćmol/l, respectively. Quantitative structure-activity relationships indicate that antiaggregatory activity is mainly affected by electronic and not by lipophilic properties of the agents. Conclusion/interpretation. Glimepiride appeared to be a more potent ADP-induced platelet aggregation inhibitor in vitro than gliclazide. Antiaggregatory activity was shown for gliquidone and confirmed for glibenclamide. The QSAR analysis supports the hypothesis of a free radical mechanism of action of sulphonylurea derivatives previously suggested for gliclazide.


    2/355

    Tytuł oryginału: Medical humanities in Central and Eastern Europe : the example of Poland
    Autorzy: Szawarski Zbigniew
    Źródło: W: Integration of medical humanities into the education of health care professionals - Vilnius, 2002 s.17-23 - Włączenie humanistyki medycznej do kształcenia personelu służby zdrowia Wilno 28-29.06. 2002 - Oddzielna numeracja stron tekstu litewskiego w odwrotnym kierunkuTytuł odwróconej odkładki także w jęz. litewskim
    Sygnatura GBL: 555,550

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca przeglądowa
  • praca opublikowana za granicą
  • tytuł obcojęzyczny


    3/355

    Tytuł oryginału: The immunophenotypic and immunogenotypic B-cell differentiation arrest in bone marrow of RAG-deficient SCID patients corresponds to residual recombination activities of mutated RAG proteins.
    Autorzy: Noordzij Jeroen G., Bruin-Versteeg Sandra de, Verkaik Nicole S., Vossen Jaak M. J. J., Groot Ronald de, Bernatowska Ewa, Langerak Anton W., Gent Dik C. van, Dongen Jacques J. M. van
    Źródło: Blood 2002: 100 (6) s.2145-2152, il., tab., bibliogr. 45 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • genetyka
  • immunologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The protein products of the recombination activating genes (RAG1 and RAG2) initiate the formation of immunoglobulin (lg) and T-cell receptors, whichare essential for B- and t-cell development, respectively. Mutations in the RAG genes result in severe combined immunodeficiency disease (SCID), generally characterized by the absence of mature B and T lymphocytes, but presence of natural killer (NK) cells. Biochemically, mutations in the RAG genes result either in nonfunctional proteins or in proteins with partial recombination activity. The mutated RAG genes of 9 patients from 7 families were analyzed for their recombination activity using extrachromosomal recombination substrates, rearrangment of endogenous Ig loci in RAG gene-transfected nonlymphoid cells, or the presence of Ig gene rearrangements in bone marrow (BM). Recombination activity was virtually absent in all 6 patients with mutations in the RAG core domains, but partial activity was present in the other 3 RAG-deficient patients, 2 of them having Omenn syndrome with oligocional T lymphocytes. Using 4-color flow cytometry, we could define the exact stage at which B-cell differentiation was arrested in the BM of 5 RAG-deficient SCID patients. In 4 of 5 patients, the absence of recombination activity was associated with a complete B-cell differentiation arrest at the transition from cytoplasmic (Cy) Igć- pre -B-I cells to Cylgć+ pre -B-II cells. However, the fifth patient showed low frequencies of precursor B cells with Cylgć and surface membrane IgM...


    4/355

    Tytuł oryginału: Comparative analysis of Ig and TCR gene rearrangements at diagnosis and at relapse of childhood precursor-B-ALL provides improved strategies for selection of stable PCR targets for monitoring of minimal residual disease.
    Autorzy: Szczepański Tomasz, Willemse Marja J., Brinkhof Bas, Wering Elisabeth R. van, Burg Mirjam van der, Dongen Jacques J. M. van
    Źródło: Blood 2002: 99 (7) s.2315-2323, il., tab., bibliogr. 47 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • pediatria
  • genetyka
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • niemowlęta
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.

    Streszczenie angielskie: Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements are excellent patient-specific polymerase chain reaction (PCR) targets for detection of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL), but they might be unstable during the disease course. Therefore, we performed detailed molecular studies in 96 childhood precusor-B-ALL at diagnosis and at relapse (n = 91) or at presumably secondary acute myeloid leukemia (n = 5). Clonal Ig and TCR tatgets for MRD detection were identified in 94 patients, with 71 p.c. of these targets being preserved at relapse. The best stability was found for IGK-Kde rearrangements (90 p.c.), followed by TCRG (75 p.c.), IGH (64 p.c.), and incomplete TCRD rearrangements (63 p.c.). Combined Southern blot and PCR data for IGH, IGK-Kde, and TCRD genes showed significant differences in stability at relapse between monoclonal and oligoclonal rearrangements: 89 p.c. versus 40 p.c., respectively. In 38 p.c. of patients all MRD-PCR targets were preserved at relapse, and in 40 p.c. most of the targets (ň 50 p.c.) were preserved. In 22 p.c. of patients most targets (10 cases) or all targets (10 cases) were lost at relapse. The latter 10 cases included 4 patients with secondary acute myeloid leukemia with germiline Ig/TCR genes. In 5 other patients additional analyses proved the clonal relationship between both disease stages. Finally, in 1 patient all Ig/TCR gene rearrangements were completely different between diagnosis and relapse, which is suggestive of secondary ALL...


    5/355

    Tytuł oryginału: Human leukocyte antigens class II and tumor necrosis factor genetic polymorphisms are independent predictors of non-Hodgkin lymphoma outcome.
    Autorzy: Juszyczyński Przemysław, Kalinka Ewa, Bienvenu Jacques, Woszczek Grzegorz, Borowiec Maciej, Robak Tadeusz, Kowalski Marek, Lech-Maranda Ewa, Baseggio Lucile, Coiffier Bertrand, Salles Gilles, Warzocha Krzysztof
    Źródło: Blood 2002: 100 (8) s.3037-3040, il., tab., bibliogr. 23 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • genetyka
  • onkologia
  • hematologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie polskie: at least 2 inherited factors involved in NHL outcome.

    Streszczenie angielskie: Tumor necrosis factor (TNF) production and non-Hodgkin lymphoma (NHL) outcome was found to be related to the TNF-308 polymorphism. To explore whether this could be linked to neighboring polymorphisms, we genotyped the TNF-376,-308,-238,-163, lymphotoxin alpha (LTŕ)+252, and HLA DRB1 alleles in 204 patients with NHL and 120 controls. TNF-308A was the only allele associated with higher TNF and its p55 and p75 receptors' levels (P=.009, P=.03, and P=.007) and lower complete remission rates (P=.006). Freedom from progession (FFP) and overall survival (OS) were shorter in patients with TNF-308A (P=.009 and P=.02), null HLA DRB1*02 allele (P=.007 and P.14) or both genetic markers (P=.004 and P.005). Multivariate analysis incorporating international. Prognostic index (IPI) identified TNF-308A (P .0001, relative risk (RR) = 1.63; P .0001, RR=1.51) and null HLA DRB1*02 alleles (P=.015, RR - 1.18; P .0001, RR = 1.25) as independent factors for FFP and OS. These results indicate the existence of least 2 inherited factors involved in NHL outcome. (Blood. 2002; 100 : 3037-3040).


    6/355

    Tytuł oryginału: Modulation of the activity of calcium-activated neutral proteases (calpains) in chronic lymphocytic leukemia (B-CLL) cells.
    Autorzy: Witkowski Jacek M., Żmuda-Trzebiatowska Emilia, Świercz Jakub M., Cichorek Mirosława, Ciepluch Hanna, Lewandowski Krzysztof, Bryl Ewa, Hellmann Andrzej
    Źródło: Blood 2002: 100 (5) s.1802-1809, il., bibliogr. 57 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Decreased susceptibility to apoptosis and impaired proliferative control are throught to be responsible for prolonged life spain and accumulation of chronic lymphocytic leukaemia (B-CLL) cells. The acitivity of calpains (calcium-dependent, neutral proteases, active in the cells responding to signals inducing a rise of cytoplasmic (Ca++) is involed in the regulation of apoptosis of some cell types by interaction with caspase-3. This work verifies the hypothesis of the abnormal activity of calpains and its role in reduced apoptosis of the BCL cells. Casein zymography, reverse transcriptase-polymerase chain reaction, and Western blotting were used for identification and quantification of the activity and expression of calpains (calcium-dependent, neutral proteases, active in the cells responding to signals inducing a rise of cytoplamic (Ca++) is involved in the regulation of apoptosis of some cell types by interaction with caspase-3. This work verifies the hypothesis of the abnormal activity of calpains and its role in reduced apoptosis of the B-CLL cells. Casein zymography, reverse transcriptase-polymerase chain reaction, and Western blotting were used for identification and quantification of the activity and expression of calpains in B-CLL cells and purified B lymphocytes. The activity and expression of ć-calpain (requiring micromolar Ca++ for activation) are significantly higher in the leukemic than in nonmalignant cells. Conctrarily, the activity and expression of m-calpain (requiring milimolar Ca++) as well as the expression of calpastatin (an endogenous inhibitor of...


    7/355

    Tytuł oryginału: Outcome of adult patients with T-lymphoblastic lymphoma treated according to protocols for acute lymphoblastic leukemia.
    Autorzy: Hoelzer Dieter, G”kbuget Nicola, Digel Werner, Faak Thomas, Kneba Michael, Reutzel Regina, Romejko-Jarosińska Joanna, Zwoliński Jacek, Walewski Jan
    Źródło: Blood 2002: 99 (12) s.4379-4395, il., tab., bibliogr. 30 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • pediatria
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We treated 45 adult patients with T-lymphoblastic lymphoma (T-LBL) (age range 15-61 years) with 2 protocols designed for adult acute lymphoblastic leukemia (ALL). An encouraging cure rate of 90 p.c. was recently reported for T-LBL in children treated with a similar approach. In our study an 8-drug standard induction was administered over 8 weeks including prophylactic cranial (24 Gy) and mediastinal irradiation(24 Gy) followed by consolidation and reinduction therapy. AT diagnosis, 91 p.c. of the 45 patients showed a mediastinal tumor and 40 p.c. had pleural/pericardial effusions: 73 p.c. of the patients had stage III/IV disease. Ovarall 42 patients (93 p.c.) achieved a complete remission (CR), 2 patients (4 p.c.) achieved a partial remission, and 1 patient (2 p.c.) died during induction. In patients with stage I-III disease (n=18) the CR rate was 100 p.c. compared with 89 p.c. in stage IV (n=27). There were 15 patients who relapsed (36 p.c.) within 12-months. The majority of relapses (47 p.c.) occurred in the mediastinum (n=7) despite mediastinal irradiation with 24 Gy in 6 out of 7 patients. The estimates for overall survival, continuous CR, and disease-free survival at 7 years are 51 p.c., 65 p.c., and 62 p.c., respectively. Stage, age, lactate dehydrogenase and all other parameters had no influence on achievement of CR or outcome. This study demonstrates in a large cohort of patients with adult T-LBL that a high CR rate and a favourable outcome can be achieved with an ALL-type regimen. Mediastinal recurrence was the major obstacle and further improvement...


    8/355

    Tytuł oryginału: Complementary functions of the antiapoptotic protein A1 and serine/threonine kinase pim-1 in the BCR/ABL-mediated leukemogenesis.
    Autorzy: Nieborowska-Skorska Małgorzata, Hoser Grażyna, Kossev Plamen, Wasik Mariusz A., Skorski Tomasz
    Źródło: Blood 2002: 99 (12) s.4531-4539, il., bibliogr. 68 poz.
    Sygnatura GBL: 301,770

    Hasła klasyfikacyjne GBL:
  • onkologia
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • zwierzęta
  • myszy
  • in vitro

    Streszczenie angielskie: BCR/ABL oncogenic tyrosine kinase activates STAT5, which plays an important role in leukemogenesis. The downstream effectors of the BCR/ABL- STAT5 pathway remain poorly defined. We show here that expression of the antiapoptic protein A1, a member of the Bcl-2 family, and the serine/threonine kinase pim-1 are enhanced by CRB/ABL. This up-regulation requires activation of STAT5 by the signaling from SH3 + SH2 domains of the BCR/ABL. Enhanced expression of A1 and pim-1 played a key role in the BCR/ABL-mediated cell protection from apoptosis. In addition, pim-1 promoted proliferation of the BCR/ABL-transformed cells. Both A1 and pim-1 were required to induce interleukin 3-independent cell growth, inhibit activation of caspase 3, and stimulate cell cycle progression. Moreover, simultaneous up-regulation of both A1 and pim-1 was essential for in vitro transformation and in vivo leukemogenesis mediated by BCR/ABL. These data indicate that induction of A1 and pim-1 expression may play a critical role in the BCR/ABL - depenendent transformation.


    9/355

    Tytuł oryginału: Early detection of COPD by high-risk population spirometric screening.
    Autorzy: Zieliński J.
    Źródło: Eur. Respir. Rev. 2002: 12 (86/87) s.287-288
    Sygnatura GBL: 313,671

    Hasła klasyfikacyjne GBL:
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca związana ze zjazdem
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska


    10/355

    Tytuł oryginału: Inhibition of alkaline phosphatase activity of boar semen by pentoxifylline, caffeine, and theophylline.
    Autorzy: Głogowski Jan, Danforth Douglas R., Ciereszko Andrzej
    Źródło: J. Androl. 2002: 23 (6) s.783-792, il., bibliogr. s. 790-792
    Sygnatura GBL: 313,131

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: Methyl xanthines have been used frequently as additives to sperm suspensions in order to improve sper characteristics. The mechanism of action on spermatozoa is generally assumed to be inhibition of sperm phospodiesterase activity, resulting in elevation of complementary adenosine monophosphate levels in spermatozoa. The present study was designed to examine the effect of methyl xanthines (pentoxifylline, caffeine, and theophylline) on another important enzyme system, alkaline phosphatase, in boar seminal plasma and spermatozoa. Inhibition of sperm alkaline phosphatase could be distinguished from that of seminal plasma by a paradoxical stimulation by pentoxifylline at lower pH values in spermatozoa. Among the three methyl xanthines, theophylline exhibited the most dramatic inhibition of alkaline phosphatase activity and substrate inhibition was observed with increasing concentrations. Each methyl xanthine had a different action on alkaline phosphatase activity at lower pH; theophylline showed the highest inhibition, caffeine inhibition was not related to pH, and pentoxifylline did not inhibit alkaline phosphatase of seminal plasma and, in fact, it stimulated its activity (or that of a phosphatase with lower pH optimum) in spermatozoa. These results indicate another possible mechanism of action of methyl xanthines on sperm and are in agreement with data indicating that methyl xanthines are not specific inhibitors of sperm phosphodiesterase, because clearly, they inhibit alkaline phosphatase activity as well.


    11/355

    Tytuł oryginału: DOTS Expansion Plan to Stop TB in the WHO European Region 2002-2006
    Opracowanie edytorskie: Danzon Marc (przedm.).
    Źródło: - [Cophenhagen, World Health Organization 2002], V, 46 s. : il., tab., 30 cm.
    Sygnatura GBL: 291,084

    Hasła klasyfikacyjne GBL:
  • pulmonologia
  • choroby zakaźne

    Typ dokumentu:
  • praca epidemiologiczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny


    12/355

    Tytuł oryginału: Relationship between acute and late normal tissue injury after postoperative radiotherapy in endometrial cancer.
    Autorzy: Jereczek-Fossa Barbara A., Jassem Jacek, Badzio Andrzej
    Źródło: Int. J. Radiat. Oncol. Biol. Phys. 2002: 52 (2) s.476-482, il., tab., bibliogr. 49 poz.
    Sygnatura GBL: 312,578

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo
  • onkologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Purpose: To evaluate the relationship between acute and late normal tissue reaction in 317 consecutive endometrial cancer patients treated with surgery and adjuvant radiotherapy (RT). Methods: The data of 317 patients (staging according to the International Federation of Gynecology and Obstetrics) treated with postoperative RT were analyzed. Both low-dose-rat brachytherapy and external beam RT were applied in 247 patients (78 p.c.); brachytherapy only in 49 (15 p.c.) and external beam irradiation only in 21 (7 p.c.). The median follow-up was 7.3 years (range 4-21). The European Organization for Research and Treatment of Cancer, Radiation Therapy Oncology Group system with elements of the late effects of normal tissue, sibjective, objective, management, analytic (LENT/SOMA) scale was used to score the RT reactions. The correlation between the occurrence and severity of acute and late bowel and bladder toxicity, as well as the relationship between the severity of acute effects and time to occurrence of late reactions, were assessed using linear and logistic regression analyses. Results: Of the 317 patients, 268 (85 p.c.) experienced acute RT reactions of any grade. Severe acute bowel reactions were observed in 15 patients (5 p.c.), urinary bladder complications in 1 patient (0.5 p.c.) cutaneous in 1 patient (0.5 p.c.), and vaginal in 1 patient (0.5 p.c.). Severe acute hematologic toxicity was seen in 3 patients (1 p.c.). A total of 158 patients (51 p.c.) experienced late RT reactions of any grade. Severe late bowel reactions were obsrved in 19 patients (6 p.c.), urinary bladder in 5 (2 p.c.), vaginal in 3 (1 p.c.), and bone in 10 (4 p.c.)...


    13/355

    Tytuł oryginału: Detection of enterotoxigenic Clostridium perfringens with a duplex PCR.
    Autorzy: Augustynowicz E[wa], Gzyl A., Ślusarczyk J.
    Źródło: J. Med. Microbiol. 2002: 51 (2) s.169-172, il., tab., bibliogr. 24 poz.
    Sygnatura GBL: 305,350

    Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Two sets of primers sedigned to detect Clostridium perfringens phospholipase C (plc) and enterotoxin (cpe) genes in a single PCR reaction were applied to a collection of 64 predominantly food poisoning-related C. perfringens isolates. In-vitro enterotoxin synthesis was tested serologically after inducing sporulation. Of the 64 isolates, 26 were clearly enterotoxigenic: 16 were classified as potentially enterotoxigenic only as serological testing did not confirm enterotoxin production, Duplex PCR for diagnosis of enteroxigenic C. perfringens from vegetative cultures can be a useful tool as fresh isolates often sporulate poorly or not all, giving rise to the possibility of false negative results by serological analysis.


    14/355

    Tytuł oryginału: Free radicals-mediated induction of oxidized DNA bases and DNA-protein cross-links by nickel chloride.
    Autorzy: Woźniak Katarzyna, Błasiak Janusz
    Źródło: Mutat. Res 2002: 514 (1/2) s.233-243, il., tab., bibliogr. 50 poz.
    Sygnatura GBL: 304,952

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • genetyka

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć męska
  • in vitro

    Streszczenie angielskie: Using the comet assay, we showed that nickel chloride at 250-1000 ćM induced DNA damage in human lymphocytes, measured as the change in comet tail moment, which increased with concentration up to 500 ćM and then decreased. Observed increase might follow from the induction of strand breaks or/and alkali-labile sites (ALS) by nickel, whereas decrease from its induction of DNA-DNA and/or DNA-protein cross-links. Proteinase K caused an increase in the tail moment, suggesting that nickel chloride at 1000 ćM might cross-link DNA with nuclear proteins. Lymphocytes exposed to NiCl2 and treated with enzymes recognizing oxidized and alkylate bases: endonuclease III (Endo III), formamidopyrimidine-DNA glycosylase (Fpg) and 3-methyladenine-DNA glycosylase II (AlkA), dusplayed greated extent of DNA damege than those not treated with these enzymes, indicating the induction of oxidized and alkylated bases by nickel. The incubation of lymphocytes with spin traps, 5,5-dimethyl-pyrroline N-oxide (DMPO) and PBN decreased the extent of DNA damage which might follow from the production of free radicals by nickel. The pre-treatment with Vitamin C at 10 ćM and Vitamin E at 25 ćM decreased the tail moment of the cells exposed to CiCl2 at the concentrations of the metal causing strand breaks or/and ALS. The results obtained suggest that free radicals may be involved in the formation of strand breaks or/and ALS in DNA as well as DNA-protein cross-links induced by NiCl2. Nickel chloride can also alkulate DNA bases. Our results support thesis on multiple, free radicals-based genotoxicity pathways of nickel.


    15/355

    Tytuł oryginału: Comparative studies of osteoblast and fibroblast type I collagen in a patient with osteogenesis imperfecta type IV.
    Autorzy: Galicka Anna, Wołczyński Sławomir, Gińdzieński Andrzej
    Źródło: J. Pathol. 2002: 196 (2) s.235-237, il., bibliogr. 16 poz.
    Sygnatura GBL: 302,048

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: The expression of type I collagen has been compared in fibroblast and osteoblast cultures of a patient with moderately severe osteogenesis imperfecta (OI) type IV, with respect to control cells. Electrophoretic analysis of type I collagen showed that both OI osteoblasts and fibroblasts synthesized normal chains with delayed migration. However, the osteoblasts contained a higher proportion of abnormal chains than fibroblasts from the proband. Pulse-chase experiments showed that the trimers containing abnormal chains were cleared more rapidly from osteoblasts than fibroblasts. Moreover, the collagen secreted by OI osteoblests had thermal stability 1řC higher than collagen secreted by OI fibroblasts. These results suggest that the abnormal collagen in osteoblasts may be more resistant to intra- and extracellular degradation and my thus have better survival than in fibroblasts. This finding could have implications for understanding the clinical phenotype of OI.


    16/355

    Tytuł oryginału: Potentiating antitumor effects of a combination therapy with lovastatin and butyrate in the Lewis lung carcinoma model in mice.
    Autorzy: Giermasz Adam, Makowski Marcin, Kozłowska Ewa, Nowis Dominika, Maj Małgorzata, Jalili Ahmad, Feleszko Wojciech, Wójcik Cezary, Dąbrowska Anna, Jakóbisiak Marek, Gołąb Jakub
    Źródło: Int. J. Cancer 2002: 97 (6) s.746-750, il., bibliogr. 32 poz.
    Sygnatura GBL: 305,029

    Hasła klasyfikacyjne GBL:
  • farmacja
  • pulmonologia
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy
  • in vitro

    Streszczenie angielskie: Lovastatin, the drug used for the treatment of hypercholesterolemia, has previously been reported to exert antitumor activity in experimental murine models. Byturate and butyric acid derivatives are well known to induce differentiation and apoptosis of tumour cells and also have recently gained acceptance as potentaial anticancer agents. In this study, we examined the antitumor effects of the combination of lovastatin and butyrate or its prodrug tributyrin in vitro and in vivo against a murine Lewis lung carcinoma (3LL). This combination therapy showed synergistic antitumor activity against 3LL cells in vitro. These effects were at least in part due to apoptosis induction that occurred after 12 hr of incubation with lovastatin and butyrate and was preceded by changes in cell cycle distribution of treated cells and expression of p21, p53 and cyclin D 1. Remarkably, a systemic treatment of syngeneic mice inoculated with 3LL cells with both drugs resulted in significant tumour growth retardation.


    17/355

    Tytuł oryginału: Accuracy of transcranial color Doppler ultrasonography in the diagnosis of middle cerebral artery spasm determined by receiver operating characteristic analysis.
    Autorzy: Mariak Zenon, Krejza Jarosław, Świercz Mirosław, Kordecki Kazimierz, Lewko Janusz
    Źródło: J. Neurosurg. 2002: 96 (2) s.323-330, il., tab., bibliogr. 68 poz.
    Sygnatura GBL: 301,435

    Hasła klasyfikacyjne GBL:
  • kardiologia
  • neurologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Object. The value of transcranial Doppler ultrasonography for the detection of middle cerebral artery (MCA) spasm has been asserted. None of the published studies, however, has adequately scrutinized the overall diagnostic accuracy of this procedure. There are only sporadic reports concerning the utility of transcranial color Doppler (TCCD) ultrasonography, although this method has been proved to be more precise. In this study the authors attempted to estimate the performance of TCD ultrasonography in detecting MCA narrowing by using receiver operating characteristic (ROC) curve analysis, based on TCCD studies obtained in a relatively large, randomly selected population of patients. Methods. Transcranial color Doppler ultrasonography studies were obtained in 100 consecutive patients (54 men and 46 women ages 18-74 years, median age 50 years) routinely referred by neurosurgeons for intraarterial angiography. The M1 segment of the MCA was insonated using a 2.5-MHz probe via a temporal acoustic window, and angle-corrected flow velocities were obtained. Angiographically depicted vasospasm was graded as none, mild (ó 25 p.c. vessel caliber reduction), and moderate to severe ( 25 p.c. vessel caliber reduction). The effectiveness of TCCD ultrasonography in diagnosing MCA spasm was evaluated by calculating the areas under the ROC curves (Az). Of the 200 MCAs examined, 173 were successfully visualized with the aid of TCCD ultrasonography. Mild vasospasm was angiographically diagnosed in 15 arteries and moderate-to-severe vasospasm in 28...


    18/355

    Tytuł oryginału: Expression of FasL gene in T cells of renal allograft recipients.
    Autorzy: Kasprzycka Monika, Kłodos Konrad, Nowaczyk Maria, Wyzgał Janusz, Podobińska Iwona, Durlik Magdalena, Górski Andrzej
    Źródło: Immunol. Lett. 2002: 80 (1) s.9-13, il., tab., bibliogr. 17 poz.
    Sygnatura GBL: 312,663

    Hasła klasyfikacyjne GBL:
  • genetyka
  • nefrologia
  • transplantologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: FasL molecule expressed on activated T cells induces apoptosis in Fas-expressing cells. It is possible that apoptosis induced by FasL is involved in the process of allograft destruction brought about by infiltrating T cells. The aim of our study was to evaluate expression of FasL gene in peripheral blood T cells of renal allograft recipients (RAR). We have studied 25 patients: 16 with uneventful stable course (RAR-S) and nine during biopsy proven chronic rejection (RAR-CH). The relative expression of FasL mRNA compared with that of á-actin was established by semi-quantitive RT-PCR. We have found that FasL gene expression was significantly increased in T cells of RAR-CH compared to RAR-S (P 0.01). Our results suggest that T cells expression of FasL gene is increased during chronic rejection. Therefore, this phenomenon may pay a role in allograft injury associated with that process. Further studies are needed to unravel possible clinical consequences of observed differences in T cell expression of FasL.


    19/355

    Tytuł oryginału: Effects of oestrogen deprivation on interleukin-6 production by peripheral blood mononuclear cells of postmenopausal women.
    Autorzy: Rachoń D., Myśliwska J., Suchecka-Rachoń K., Więckiewicz J.
    Źródło: J. Endocrinol. 2002: 172 (2) s.387-395, il., tab., bibliogr. s. 393-395
    Sygnatura GBL: 310,833

    Hasła klasyfikacyjne GBL:
  • immunologia
  • endokrynologia
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Various hormones can influence the expression of interleukin-6 (IL-6) and oestrogens are the most extensively studied. There is, however, controversy about the nature of the IL-6 secreted by human cells and its regulation by 17á-oestradiol. The aim of this work was to clarify whether oestrogen deprivation after menopause may contribute to an enhanced IL-6 production by peripheral blood mononuclear cells (PBMC) in postmenopausal women. Twenty-two healthy postmenopausal women, age range 45-63 years with clinical symptoms of oestrogen deficiency were enrolled in the study. The control group consisted of 16 healthy young women, age range 22-31 years, with regular menses and who were not taking oral contraceptives. Levels of IL-6 in the sera and PBMC culture supernatants were measured by the biological B9 cell-proliferation assay and expression of the IL-6 gene in non-stiulated PBMC was detected by RT-PCR. The effect of 17á-oestradiol on spontaneous IL-6 production by the PBMC of postmenopausal women was also studied in vitro and in vivo. Seventeen out of the twenty-two postmenopausal women were given hormonal replacement therapy of 50 ćg 17á-oestradiol/day transdermally and the spontaneous production of IL-6 by the PBMC was analysed after 6 and 12 months of treatment. The postmenopausal women had significantly higher serum levels of IL-6 than the young controls. The spontaneous production of IL-6 by non-stimulated PBMC into the culture supernatants was also significantly higher in the postmenopausal women compared with the young...


    20/355

    Tytuł oryginału: Myocardial perfusion correlates with improvement of systolic function of the left ventricle after CABG. Dobutamine echocardiography and Tc-99m-MIBI SPECT study.
    Autorzy: Paluszkiewicz L., Kwinecki P., Jemielity M., Dyszkiewicz W[ojciech], Cieśliński A., Szyszko A.
    Źródło: Eur. J. Cardiothorac. Surg. 2002: 21 (1) s.32-35, tab., bibliogr. 20 poz. - 14 Doroczne Spotkanie Europejskiego Towarzystwa Chirurgii Klatki Piersiowej i Serca Frankfurt 07-11.10. 2000
    Sygnatura GBL: 312,899

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • kardiologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: The aim of the study was to assess the effect of surgical revascularization [coronary artery bypass grafting (CABG)] on systolic function and perfusion of the left ventricle using dobutamine echocardiography (DE) and Tc-99m-MIBI SPECT (SPECT). Methods: There were 32 patients mean age 52.2 ń 7.2 years in whom DE and SPECT were performed before and 3-4 months after CABG using standard protocols. Wall motion score index (WMSI) and perfusion index (PI) were calculated. Results: Significant improvement of WMSI at rest (1.44 ń 0.46 vs 1.33 ń 0.41; P = 0.03) as well as after maximal dose of dobutamine (1.49 ń 0.42 vs 1.30 ń 0.44; P = 0.02) was observed after CABG as compared to preoparative examination. Similar relation was observed during SPECT study. Perfusion index diminished significantly after revascularization during rest acquisition (2.19 ń 0.71 vs 1.93 ń 0.70; P = 0.0008) and after Dipirydamole administration (2.73 ń 0.73 vs 2.20 ń 0.69; P = 0.0001) as compared to preoperative examination. We found correlation between PI and WMSI at rest before CABG (R = 0.46; P = 0.01). PI after Dipirydamole and WMSI after maximal dose of Dobutamine before CABG (R = 0.37; P = 0.04), PI and WMSI at rest CABG (R = 0.39; P = 0.03), PI after Dipirydamole and WMSI after dobutamine after CABG (R = 0.38; P = 0.03). Conclusions: Surgical revascularization significantly improves both perfusion and contractility. Increased perfusion after CABG correlates with improvement of systolic function of the left ventricle.


    21/355

    Tytuł oryginału: A new type of mixed anhydride and its applications to the synthesis of 7-substituted 8-chloro-5,5-dioxoimidazo[1,2-b][1,4,2]benzodithiazines with in vitro antitumor activity.
    Autorzy: Brzozowski Zdzisław, Sączewski Franciszek
    Źródło: J. Med. Chem. 2002: 45 (2) s.430-437, il., tab., bibliogr. 39 poz.
    Sygnatura GBL: 312,133

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: A new series of 8-chloro-5,5-dioximidazo[1,2-b][1,4,2]benzodithiazine derivatives 8-25 with heteroaryloxycarbonyl or heteroarylcarbamoyl substituents at position 7 have been synthesized as potential antitumor agents. In this procedure a novel type of mixed anhydride 7 was prepared from 8-chloro-5,5-dioxoimidazo[1,2-b][1,4,2]benzodithiazine-7-carboxylic acid 6 and methanesulfonyl chloride, which in turn was condensed either with heteroarylamines or heteroarylhydroxy compounds. All the compounds prepared were screened at the National Cancer Institute (NCI) for their activities against a panel of 60 tumor cell lines, and relationships between structure and antitumor activity in vitro are discussed. The amides 8, 10, 12, 13, 21, and ester 25 were inactive, whereas the other compounds exhibited rather moderate activity against one or more human tumor cell lines. The prominent compound with remarkable activity (log GI50 -8) and selectivity for the leukemia HL-60(TB) cell line was 2-methyl-8quinolyl 8-chloro-5,5-dioximidazo[1,2-b][1,4,2]benzodithiazine-7-carboxylate 24.


    22/355

    Tytuł oryginału: Pulsatile blood pressure component as predictor of mortality in hypertension: a meta-analysis of clinical trial control groups.
    Autorzy: Gasowski Jerzy, Fagard Robert H., Staessen Jan A., Grodzicki Tomasz, Pocock Stuart, Boutitie Florent, Gueyffier Francois, Boissel Jean-Pierre
    Źródło: J. Hypertens. 2002: 20 (1) s.145-151, il., tab., bibliogr. 34 poz.
    Sygnatura GBL: 313,132

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca epidemiologiczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Objective. Although cirrent guidelines rest exclusively on the measurement of systolic and diastolic blood pressures, the arterial pressure wave is more precisely described as consisting of a pulsatile (pulse pressure) and a steady (mean pressure) component. This study explored the independent roles of pulse pressure and mean pressure as predictors of mortality in a wide range of patients with hypertension. Design and methods. This meta-analysis, based on individual patient data, has combined results from the control groups of seven randomized clinical trials conducted in patients with systolo-diastolic or isolated systolic hypertension. The relative hazard rates associated with pulse pressure and mean pressure were calculated using Cox's proportional hazard regression models with stratification for the seven trials and with adjustment for sex, age, smoking and the other pressure. Results. A 10 mmHg wider pulse pressure at baseline, which corresponds to approximately one-half of its standard deviation, was independently associated with an increase in risk by 6 p.c. for total mortality (P = 0.001), 7 p.c. for cardiovascular mortality (P = 0.01), and 7 p.c. for fatal coronary accidents (P = 0.03). The corresponding increase in risk of fatal stroke was similar (+ 6 p.c., P = 0.27) but there were too few strokes to reach statistical significance. In similar analyses, mean pressure was not identified as an independent predictor of these outcomes. Significant interactions of pulse pressure or mean pressure with age suggested that the prognostic power of pulse pressuer for fatal stroke was more important at higher age (P = 0.04), whereas the prognostic power of mean pressure for coronary mortality was greatest in the young (P = 0.01)...


    23/355

    Tytuł oryginału: Inhibition of N-methyl-D-aspartic acid-nitric oxide synthase in rat hippocampal slices by ethanol. Evidence for the involvement of tetrahydrobiopterin but not lipid peroxidation.
    Autorzy: Czapski Grzegorz A., Sun Grace Y., Strosznajder Joanna B.
    Źródło: J. Biomed. Sci. 2002: 9 (1) s.3-9, il., tab., bibliogr. 30 poz.
    Sygnatura GBL: 313,488

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The ionotropic glutamatergic receptor system, especially the subtype mediated by N-methyl-D-aspartic acid (NMDA), is known to exhibit special sensitivity to the effect of ethanol. This is due partly to the ability of ethanol to modulate the production of nitric oxide through the NMDA-nitric oxide synthase (NOS) pathway. In this study, we examined the effects of ethanol on basal and NMDA-stimulated NOS activity in rat hippocampal slices by measuring the conversion of [**14C]-arginine into [**14C]-citrulline in an incubation system containing the necessary cofactors. Stimulation of hippocampal slices with NMDA (100 ćM) enhanced NOS activity by 43 p.c. (n = 12). Although ethanol did not alter NOS activity when added to the incubation system during NMDA stimulation, it dose-dependently inhibited NMDA-NOS activity when added to the slices during the 90-min preincubation period. Further assay of NOS activity with brain cytosolic fraction indicated an inhibitory effect of ethanol (200 mM) when the assay was carried out in the absence of exogenous tetrahydrobiopterin (BH4), a redox-active cofactor for NOS. Incubation of brain homogenates resulted in a time-dependent increase in the levels of lipid peroxidation products, but ethanol did not further enhance these products. Taken together, these results providence evidence for the role of BH4 but not oxidative stress in the inhibitory effect of ethanol on NMDA-NOS activity in rat hippocampal slices.


    24/355

    Tytuł oryginału: Angiogenesis inhibitors specific for methionine aminopeptidase 2 as drugs for malaria and Leishmaniasis.
    Autorzy: Zhang Peng, Nicholson Diarmuid E., Bujnicki Janusz M., Su Xinzhuan, Brendle James J., Ferdig Michael, Kyle Dennis E., Milhous Wilbur K., Chiang Peter K.
    Źródło: J. Biomed. Sci. 2002: 9 (1) s.34-40, il., tab., bibliogr. 27 poz.
    Sygnatura GBL: 313,488

    Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: Methionine aminopeptidase 2(MetAP2) is responsible for the hydrolysis of the initiator methionine molecule from the majority of newly synthesized proteins. We have cloned the MetAP2 gene from the malaria parasite Plasmodium falciparum (PfMetAP2; GenBank accession number AF348320). The cloned PfMetAP2 has no intron, consists of 1,544 bp and encodes a protein of 354 amino acids with a molecular mass of 40,537 D and an overall base composition of 72.54 p.c. A + T. PfMetAP2 has 40 p.c. sequence identity with human MetAP2 and 45 p.c. identity with yeast MetAP2, and is located in chromosome 14 of P. falciparum. The three-dimensional structure of PfMetAP2 has been modeled based on the crystal structure of human MetAP2, and several amino acid side chains protruding into the binding pocket that differ between the plasmodial and human enzyme have been identified. The specific MetAP2 inhibitors, fumagilin and TNP-470, potently blocked in vitro growth of P. falciparum and Leishmania donovani, with IC50 values similar to the prototype drugs. Furthermore, in the case of P. falciparum, the chloroquine-resistant strains are equally susceptible to these two compounds.


    25/355

    Tytuł oryginału: Intrapartum cardiotocography and fetal pulse oximetry in assessing fetal hypoxia.
    Autorzy: Leszczyńska-Gorzelak B., Poniedziałek-Czajkowska E., Oleszczuk J.
    Źródło: Int. J. Gynecol. Obstet. 2002: 76 (1) s.9-14, tab., bibliogr. 20 poz.
    Sygnatura GBL: 312,925

    Hasła klasyfikacyjne GBL:
  • pediatria
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płód
  • płeć żeńska
  • ciąża

    Streszczenie angielskie: Objectives: A retrospective analysis of short-term variability (STV), a cardiotocography (CTG) parameter, in relation to fetal blood saturation values (FSpO2) obtained by fetal pulse oximetry. Methods: The study included 26 healthy pregnant women monitored continuously during delivery with both cardiotocography and fetal pulse oximetry. Results: Lower FSpO2 values were observed in the group showing STV levels ó 6.0 ms in the 1st stage of labor (44.7 ń 3.46 p.c. vs. 49.2 ń 1.8 p.c.), but this result was not statistically significant. In the 2nd stage of labor, FSpO2 values in the group with STV levels ó 6.0 ms were significantly lower than those with STV levels 6.0 ms (34.4 ń 2.9 p.c. vs. 43 ń 7.2 p.c.; P 0.001). A positive correlation was found between STV levels ó 6.0 ms and mean FSpO2 values in the 2nd stage of labor. Conclusions: 1. A significant relationship was observed between short-term variability in the cardiotocographic records and fetal blood saturation levels in the 2nd stage of labor. 2. Fetal pulse oximetry can be valuable in assessing fetal wellbeing, especially when CTG records are abnormal.


    26/355

    Tytuł oryginału: Apoptosis in the first trimester human placenta: the role in maintaining immune privilege at the maternal-foetal interface and in the trophoblast remodelling.
    Autorzy: Jerzak Małgorzata, Bischof Paul
    Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 100 (2) s.138-142, il., bibliogr. 56 poz.
    Sygnatura GBL: 305,582

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska
  • ciąża

    Streszczenie angielskie: Apoptosis has been proposed as a mechanism maintaining immune privilege. Expression of Fas ligand (FasL) by the human trophoblast has been recently accepted as a mechanism providing protection against the lytic action of activated decidual immune cells expressing Fas receptor (FasR). Therefore, the purpose of this review was determine the role of apoptosis in early pregnancy maintenance according to the latest literature. We used Medline literature search. The data suggest that apoptosis may serve as a previously unsuspected mechanism that induces tolerance of the foetal allograft against maternal immune system. Apoptosis of activated maternal immune cells occurs in the human decidua mainly through Fas-FasL or receptor for TNF-related apoptosis-inducing ligand (TRAIL-R)-TNF-related apoptosis-inducing ligand (TRAIL) signalling. This might be a defence mechanism against rejection of the foetal allograft by the maternal immune system. In addition, in this review contribution of programmed cell death to placental cell turnover and remodelling during first trimester of pregnancy is also discussed.


    27/355

    Tytuł oryginału: Reduction in fatal pulmonary embolism and venous thrombosis by perioperative administration of low molecular weight heparin. Gynecological ward retrospective analysis.
    Autorzy: Cyrkowicz Andrzej
    Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 100 (2) s.223-226, tab., bibliogr. 20 poz.
    Sygnatura GBL: 305,582

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • farmacja
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: Objectives: To compare the frequency of deep vein thrombosis (DVT) and pulmonary embolism (PE) in patients undergoing gynecological operations without low molecular weight heparin (LMWH) prophylaxis and those receiving such prophylaxis. Design: Retrospective, hospital record-based study. Material and methods: About 1785 consecutive patients without LMWH prophylaxis and 1871 ones treated with nadroparin (Fraxiparine) 7500 ICU s.c. 2 h before the operation and repeated daily for 5-7 days or until the patient was fully mobile. DVT was diagnosed on the basis of clinical symptoms and ultrasound examination, and PE clinical symptoms, gasometric data, electrocardiography and chest X-ray. Results: Among the patients without prophylaxis, four cases of PE occurred (0.22 p.c.), two fatal (0.11 p.c.), 13 cases of proximal DVT (0.72 p.c.) and 41 distal DVT complications (2.3 p.c.). In LMWH, group 3 proximal DVT (0.16 p.c.) and 18 distal DVT (0.96 p.c.) developed but there were no clinically expressed pulmonary embolism. According to the Fisher's exact test, the difference between the complications in the analyzed groups is significant P 0.05. Conclusions: The perioperative applying of LMWH to prevent DVT in the patients operated on gynecologically is effective.


    28/355

    Tytuł oryginału: Immunopharmacological potential of selective phosphodiesterase inhibition.[P.] 1: Differential regulation of lipopolysaccharide-mediated proinflammatory cytokine (interleukin-6 and tumor necrosis factor-ŕ) biosynthesis in alveolar epithelial cells.
    Autorzy: Haddad John J., Land Stephen C., Tarnow-Mordi William O., Zembala Marek, Kowalczyk Danuta, Lauterbach Ryszard
    Źródło: J. Pharmacol. Exp. Ther. 2002: 300 (2) s.559-566, il., bibliogr. s. 565-566
    Sygnatura GBL: 310,547

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • in vitro

    Streszczenie angielskie: In an attempt to elaborate in vitro on a therapeutic strategy that countracts an inflammatory signal, we previously reported a novel immunopharmacological potential of glutathione, an antioxidant thiol, in regulating inflammatory cytokines. In the present study, we investigated the hypothesis that selective regulation of phosphodiesterases (PDEs), a family of enzymes that controls intracellular cAMP/cGMP degradation, differentially regulates proinflammatory cytokines. Selective PDE1 inhibition (8-methoxymethyl-3-isobutyl-1-methylxanthine) blockaded lipopolysaccharide-endotoxin (LPS)-mediated biosynthesis of interleukin (IL)-6, but this pathway had no inhibitory effect on tumor necrosis factor-ŕ (TNF-ŕ). Furthermore, inhibition of PDE3 (amrinone) abolished the effect of LPS on IL-6, but attenuated TNF-ŕ production. Reversible competitive inhibition of PDE4 (rolipram) exhibited a potent inhibitory effect on IL-6 and a dual, biphasic (excitatory/inhibitory) effect on TNF-ŕ secretion. Blockading PDE5 (4-[3',4'-(methylenediox)benzyl]amino-6-methoxyquinazoline) showed a high potency in reducing IL-6 production, but in a manner similar to the inhibition of PDE4, exhibited a biphasic effect on TNF-ŕ biosynthesis. Simultaneous inhibition of PDE5, 6 and 9 (zaprinast), purported to specifically elevate intracellular cGMP, reduced, in a doseindependent manner, IL-6 and TNF-ŕ biosynthesis. Finally, nonselective inhibition of PDE by pentoxyfylline suppressed LPS-mediated secretion of IL-6 and TNF-ŕ. The involvement of specific PDE isoenzymes in differentially regulating LPS-mediated inflammatory cytokine biosynthesis indicates a novel approach to unravel the potential therapeutic targets that these isozymes constitute during the progression of inflammation within the respiratory epithelium.


    29/355

    Tytuł oryginału: Immunopharmacological potential of selective phosphodiesterase inhibition. [P.] 2: Evidence for the involvement of an inhibitory-kB/nuclear factor-kB-sensitive pathway in alveolar epithelial cells.
    Autorzy: Haddad John J., Land Stephen C., Tarnow-Mordi William O., Zembala Marek, Kowalczyk Danuta, Lauterbach Ryszard
    Źródło: J. Pharmacol. Exp. Ther. 2002: 300 (2) s.567-576, il., tab., bibliogr. s. 575-576
    Sygnatura GBL: 310,547

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: In this report we investigated the immunopharmacological role of selective and nonselective phosphodiesterase (PDE) inhibition in regulating the inhibitory-kB (IkB-ŕ)/nuclear factor-kB (NF-kB) signally transduction pathway. In fetal alveolar type II epithelial cells, PDE blockade at the level of the diverging cAMP/cGMP pathways differentially regulated the phosphorylation and degradation of IkB-ŕ, the major cytosolic inhibitor of NF-kB. Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kB1 (p50), RelA (p65), RelB (p68), and c-Rel (p75), selective blockade of PDE 5, 6, and 9, by the action of 4-[3',4'=(methylenedioxy)benzyl]amino)-6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS)-mediated NF-kB translocation. Pentoxifilline, a nonspecific PDE inhibitor, reversed the excitatory effect of LPS on NF-kB subunit nuclear localization, in a dosedependent manner. Furthermore, analysis of NF-kB activation under the same conditions revealed a biphasic effect mediated by LPS. PDEs 1, 3, and 4 inhibition was associated with upregulating NF-kB transcriptional activity. In contrast, blockading the activity of PDEs 5, 6, and 9 negatively attenuated LPS-mediated NF-kB activation, similar to the effect of 3,7-dihydro-3,7-dimethyl-1-(5-oxohexyl)-1H-purine-2,6-dione (pentoxifulline). These results indicate that selective and nonselective interference with the control of the dynamic equilibrium of cyclic nucleotides via PDE isoenzyme regulation represents an immunoregulatory mechanism that requires the differential, biphasic targeting of the IkB-ŕ/NF-kB pathway.


    30/355

    Tytuł oryginału: Matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1: a possible role in the pathogenesis of endometriosis.
    Autorzy: Szamatowicz J., Laudański P., Tomaszewska I.
    Źródło: Hum. Reprod. 2002: 17 (2) s.284-288, il., tab., bibliogr. s. 287-288
    Sygnatura GBL: 313,265

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.

    Streszczenie angielskie: Background: Matrix metalloproteinases (MMPs) are a family of endopeptidases which play a role in the degradation and turnover of extracellular matrix proteins. Their action is regulated by specific tissue inhibitors called tissue inhibitors of metalloproteinases (TIMPs). Methods: We measured the concentrations of total and active MMP-9 in peritoneal fluid of infertile women with mild or moderate endometriosis (n = 22) and compared them with those in a control group of infertile patients (n = 21). Results: We found that the mean (ń SD) total concentrations of MMP-9 in the peritoneal fluid of patients with endometriosis was 6.2 ń 1.8 ng/ml, in comparison with 2.9 ń 2.6 ng/ml in the control group (P = 0.001). Concenrations of active MMP-9 did not differ significantly between the groups. The concentrations of TIMP-1, after logarithmic transformation, were significantly lower (P = 0.017) in endometriotic peritoneal fluids than in peritoneal fluid of control women, 1.02 ń 0.21 ng/ml and 1.16 ń 0.18 ng/ml respectively. No correlation between stage of disease, steroid hormone concentration, MMP-9 (total and active) and TIMP-1 was found. Conclusions: These results suggest that a disturbed equilibrium exists between MMP-9 and TIMP-1 in peritoneal fluid of women with endometriosis. This may play an important role in the pathogenesis of the disease.


    31/355

    Tytuł oryginału: HA 1004, an inhibitor of serine/threonine protein kinases, restores the sensitivity of thymic lymphomas to Caý+ -mediated apoptosis through a protein kinase A-independent mechanism.
    Autorzy: Matuszyk Janusz, Cebrat Małgorzata, Kalas Wojciech, Strzadała Leon
    Źródło: Int. Immunopharmacol. 2002: 2 (4) s.435-442, il., bibliogr. 20 poz.
    Sygnatura GBL: 313,577

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Our revious reports showed that thymic lymphomas arising in TCR transgenic mice are resistant to Caý+ -mediated apoptosis. Here we show that induction of apoptosis in thymic lymphomas involves a process that is cAMP-mediated and which depends on the activation of protein kinase A (PKA) despite the lower level of PKA type I in these lymphomas compared to thymocytes. Further, we show that treatment of the lymphomas with HA1004, a serine/threonine protein kinase inhibitor, restores their susceptibility to ionomycin-induced apoptosis. Results indicate that HA1004-induced restoration of sensitivity to ionomycin proceeds through a PKA-independent mechanism. Moreover, activation of PKA instead of its inhibition induces apoptosis of lymphoma cells.


    32/355

    Tytuł oryginału: Lactoferrin immunomodulation of DTH response in mice.
    Autorzy: Actor Jeffrey K., Hwang Shen-An, Olsen Margaret, Zimecki Michał, Hunter jr Robert L., Kruzel Marian L.
    Źródło: Int. Immunopharmacol. 2002: 2 (4) s.475-486, il., tab., bibliogr. 51 poz.
    Sygnatura GBL: 313,577

    Hasła klasyfikacyjne GBL:
  • immunologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Improved nontoxic adjuvants, especially adjuvants capable of inducing cell-mediated immunity (CMI), are needed for research in immunology and for development of human and veterinary vaccines. Bovine Lactoferrin, an effector molecule shown to directly participate in host defense, was assessed at various concentrations as an adjuvant component for induction of DTH responses to sheep red blood cells (SRBC). Subcutaneous immunization with Lactoferrin enhanced delayed type hypersensitivity (DTH) in CBA mice in a dose-dependent fashion; DTH responses were most significantly increased when sensitization was accomplished using Lactoferrin at 50 ćg/dose and 250 ćg/dose. Furthermore, Lactoferrin admixed with suboptimal dose of SRBC enhanced DTH responses by over 17-fold. Peritoneal cells collected from mice intraperitoneally injected with a 100 ćg/dose of Lactoferrin demonstrated modest, but significant, production of TNF-ŕ, IL-12 and MIP-1ŕ, when cultured in vitro, compared to saline-injected controls. J774A.1 murine macrophages stimulated with Lactoferrin resulted in increased TNF-ŕ protein production, and upregulated IL-12 and IL-15 mRNA. Levels of message for chemokins MIP-1ŕ and MIP-2 were also increased in a dose-dependent way. Taken together, these results indicate that Lactoferrin as an adjuvant may stimulate macrophages to generate a local environment likely to push immune responses towards development and maintenance of CMI.


    33/355

    Tytuł oryginału: Nitric oxide and convulsions in 4-aminopyridine-treated mice.
    Autorzy: Tutka Piotr, Młynarczyk Małgorzata, Żółkowska Dorota, Kleinrok Zdzisław, Wielosz Marian, Czuczwar Stanisław J.
    Źródło: Eur. J. Pharmacol. 2002: 437 (1/2) s.47-53, il., tab., bibliogr. s. 52-53
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: We studied whether N**G-nitro-L-arginine (NNA), an inhibitor of nitric oxide (NO) synthase as well as L-arginine and molsidomine, two agents elevating NO, influenced convulsions caused by 4-aminopyridine, a K+ channel blocker in mice. NNA, in a dose known to decrease level of NO (40 mg kg -**1), enhanced the susceptibility to intraperitoneal (i.p.) and intracerebroventricular (i,c,v,) 4-aminopyride. L-arginine (500 mg kg -**1) and molsidomine (20 mg kg -**1) alone did not influence 4-aminopyridine-induced seizure activity. Surprisingly, the proconvulsant effect of NNA upon clonic and tonic seizures was potentiated by molsidomine (20 mg kg -**1). No influence of L-arginine on the proconvulsant effect of NNA was found. Taking into account the proconvulsant effect of NNA, an involvement of NO-mediated events in the mechanism of convulsive activity of 4-aminopyridine might by postulated, However, the ineffectiveness of L-arginine and molsidomine to suppress the convulsive activity of 4-aminopyridine as well as a paradoxical potentiation of the proconvulsant effect of NNA by molsidomine seem to exclude the impact of NO pathway on 4-aminopyridine-induced convulsions in mice. Our data suggest that the proconvulsant effect of NNA in this seizure model is caused by other, not related to NO, mechanisms.


    34/355

    Tytuł oryginału: Decortication in chronic pleural empyema - effect on lung function.
    Autorzy: Rzyman Witold, Skokowski Jan, Romanowicz Grzegorz, Lass Piotr, Dziadziuszko Rafał
    Źródło: Eur. J. Cardiothorac. Surg. 2002: 21 (3) s.502-507, il., bibliogr. 24 poz.
    Sygnatura GBL: 312,899

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: The aim of the present study is to evaluate the lung function before and after the lung decortication in patients with chronic pleural empyema (CPE).Methods: Twenty-six patients with diagnosis of CPE were evaluated in a prospective manner by lung perfusion scintigraphy, blood gas analysis and spirometry before and 35 weeks (ń 17) after the lung decortication. Results: Preoperative scintigraphy showed reduction of lung perfusion on the affected side to 24.5 p.c. (ń 12.6 p.c.) in 11 right side empyemas (predicted value 55 p.c.) and to 18 p.c. (ń 8 p.c.) in 15 left side empyemas (predicted value 45 p.c.). The postoperative measurements showed improvement in perfusion to 45.2 p.c. (ń 7.7 p.c.) in patients with right side empyema and 34.1 p.c. (ń 8.5 p.c.) with the left side affection. The preoperative vital capacity (VC) was reduced to 62.3 p.c. (ń 13.8 p.c.) of the predicted value and forced expiratory volume in 1 s (FEV1) to 50 p.c. (ń 15.5 p.c.) of the predicted value. Postoperatively, slight improvement was achieved to 79.8 p.c. (ń 12.9 p.c.) for VC and 69.2 p.c. (ń 12.7 p.c.) for FEV1. Blood gas analysis showed decreased value in majority of the patients before operation and significant improvement in postoperative evaluation: Conclusion: Perfusion and spirometry improves significantly in patients with CPE after the lung decortication but function of the affected lung remains impaired. There was no influence of the age, gender, side of the disease, bacteriology or duration of the empyema before operation on lung function.


    35/355

    Tytuł oryginału: 2- Chloroadenosine, a preferential agonist of adenosine A1 receptors, enhances the anticonvulsant activity of carbamazepine and clonazepam in mice.
    Autorzy: Borowicz Kinga K., Łuszczki Jarogniew, Czuczwar Stanisław J.
    Źródło: Eur. Neuropsychopharmacol. 2002: 12 (2) s.173-179, il., tab., bibliogr. [28] poz.
    Sygnatura GBL: 313,361

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: 2-Chloroadenosine (0.25 - 1 mg/kg) significantly raised the threshold for electroconvulsions in mice. This preferential adenosine A1 receptor agonist (at 0.125 mg/kg) significantly potentiated the protective activity of carbamazepine against maximal electroshock-induced seizures in mice. 2-Chloroadenosine (1 mg/kg) showed also anticonvulsive efficacy against pentylenetetrazol-evoked seizures, raising the CD50 vale for pentylenetetrazol from 77.2 to 93.7 mg/kg. The drug (at 0.5 mg/kg) significantly enhanced the protective action of clonazepam in this test, decreasing its ED50 value from 0.033 to 0.011 mg/kg. Moreover, aminophylline, a non-selective adenosine receptor antagonist (5 mg/kg), and 8-cyclopentyl-1,3-dimethylxanthine (8-CPX), a selective A1, adenosine receptor antagonist (5 mg/kg) reversed the 2-chloroadenosine (0.125 mg/kg)-induced enhancement of the protective activity of carbamazepine and clonazepam. 2-Chloroadenosine administered alone or combined with antiepileptic drugs, caused neither motor nor long-term memory impairment. Finally, the adenosine A1 agonist did not change the free plasma concentration of antiepileptic, so a pharmacokinetic factor is not probable. Summing up, 2-chloroadenosine potentiated the protective activity of both carbamazepine and clonazepam, which seems to be associated with the enhancement of purinergic transmission mediated through adenosine A1 receptors.


    36/355

    Tytuł oryginału: The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines. P. 1: Reduction of cytostatic effects of quinacrine mustard by caffeine.
    Autorzy: Kapuściński Jan, Ardelt Barbara, Piosik Jacek, Zdunek Małgorzata, Darzynkiewicz Zbigniew
    Źródło: Biochem. Pharmacol. 2002: 63 (4) s.625-634, il., tab., bibliogr. 45 poz.
    Sygnatura GBL: 304,395

    Hasła klasyfikacyjne GBL:
  • genetyka
  • farmacja

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Recently, accumulated statistical data indicate the protective effect of caffeine consumption against several types of cancer diseases. There are also reports about protective effect of caffeine and other xanthines against tumors induced by polycyclic aromatic hydrocarbons. One of the explanations is based on biological activation of such carcinogens by cytochromes that are also known for metabolism of caffeine. However, there is also numerous data indicating reverse effect on cytotoxicity of anticancer drugs that inhibit the action of topoisomerase I (e.g. Camptothecin or Topotecan) and topoisomerase II inhibitors (e.g. Doxorubicin, Mitoxantrone or mAMSA). In this work we tested the hypothesis that the caffeine protective effect is the result of sequestering of aromatic mutagens by formation of stacking (ă - ă) complex. As the models for the study we have chosen two well-known mutagens, that do not require metabolic activation: quinacrine mustard (QM, aromatic, heterocyclic nitrogen mustard) and mechlorethamine (NM2, aliphatic nitrogen mustard). The flow cytometry study of these agents' action on the cell cycle of HL-60 cells indicated that caffeine prevents the cytotoxic action of QM, but not that of NM2. The formations of stacking complexes of QM with caffeine were confirmed by light absorption, calorimetric measurements and by molecular modeling calculation. Using the statistical thermodynamics calculations we calculated the "neighborhood" association constant (KAC = 59 ń 2 M**-1) and enthalpy change (DeltaH**0' = -116 cal mol**-1); the favorable entropy change of complex formation (DeltaS**0' = 7.72 cal mol**-1 K**-1, ...


    37/355

    Tytuł oryginału: The modulation by xanthines of the DNA-damaging effect of polycyclic aromatic agents. P. 2: The stacking complexes of caffeine with doxorubicin and mitoxantrone.
    Autorzy: Piosik Jacek, Zdunek Małgorzata, Kapuściński Jan
    Źródło: Biochem. Pharmacol. 2002: 63 (4) s.635-646, il., tab., bibliogr. 45 poz.
    Sygnatura GBL: 304,395

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Recently accumulated statistical data indicate the protective effects of caffeine consumption against several types of cancer diseases. There are also reports, about protective effect of caffeine and other xanthines against tumors induced by polycyclic aromatic hydrocarbons. One of the explanations of this phenomenon is based on biological activation of such carcinogens by cytochromes that are also known for metabolism of caffeine. In the accompanying paper [Kapuścinski et al., this issue] we provide evidence (flow cytometry and the cell cycle analysis) that the cytostatic effects of caffeine (CAF) on two DNA alkylating agents, which do not require the biological activation, depend on their ability to form stacking (ă - ă) complex. In this study, we use physicochemical techniques (computer aided light absorption and microcalorimetry), and molecular modeling to examine previously published qualitative data. This is published both by our and other group's data, indicates that CAF is able to modify the cytotoxic and/or cytostatic action of the two well known antitumor drugs doxorubicin (DOX) and mitoxantrone (MIT). To obtain the quantitative results from the experimental data we used the statistical-thermodynamical model of mixed aggregation, to find the association constants KAC of the CAF-drug interaction (128 ń 10 and 356 ń 21 M**-1 for DOX-CAF and MIT-CAF complex formation, respectively). In addition, the favorable enthalpy change of CAF-MIT (DeltaH = -11.3 kcal/mol) was measured by microcalorimetry titration. The molecular modeling (semi-empirical and force field method)...


    38/355

    Tytuł oryginału: Assessment of the quality and frequency of mutations occurrence in PTEN gene in endometrial carcinomas and hyperplasias.
    Autorzy: Konopka Bożena, Paszko Zygmunt, Janiec-Jankowska Aneta, Goluda Marian
    Źródło: Cancer Lett. 2002: 178 (1) s.43-51, il., tab., bibliogr. 30 poz.
    Sygnatura GBL: 306,023

    Hasła klasyfikacyjne GBL:
  • genetyka
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: The quality and frequency of mutations in PTEN gene were assessed in 59 carcinomas and 6 hyperplasias of the endometrium in women. Screening for mutations was done in all exons of PTEN gene by the PCR-SSCP analysis and DNA sequencing. Results were correlated with histological status and clinical features of endometrial carcinomas. In 45.8 p.c. (27/59) of carcinomas, 36 somatic mutations were detected in PTEN gene. In seven carcinomas, two mutations and in one carcinoma there mutations coexisted simultaneously. Moreover in 33.3 p.c. (2/6) of hyperplasia cases mutations were shown. Most identified mutations (57.9 p.c.) were present in exons 5 and 8, less frequently in exons 2 (15.8 p.c.) and 7 (13.2 p.c.) and they were least frequent in exons 1 and 3 (5.3 p.c. each). No mutations were found in exons 4, 6 and 9. Of all identified mutations, 73.7 p.c. of these resulting in truncated protein were present due to deletions, insertions and nonsense mutations. Missense mutations accounted for 13.2 p.c. of mutations and they were present only in exon 5. One point mutation (2.5 p.c.) was in intronic splice site. The remaining 10.5 p.c.of mutations were neutral polymorphisms. No statistically significant correlation were found between the frequency of PTEN gene mutations and the clinical stage of endometrial carcinomas. However, evident statistically significant, reverse correlation were observed between the frequency of mutations and the grade of morphological differentation of the diseases (Xý = 7.2393, ŕ = 0.0071). In conclusion, our data support the view that PTEN gene mutations are frequent events involved in development of endometrial carcinomas in women.


    39/355

    Tytuł oryginału: Accuracy of linear endoscopic ultrasonography in the evaluation of patients with suspected common bile duct stones.
    Autorzy: Kohut M., Nowakowska-Duława E., Marek T., Kaczor R., Nowak A.
    Źródło: Endoscopy 2002: 34 (4) s.299-303, tab., bibliogr. 28 poz.
    Sygnatura GBL: 305,439

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background and study aims: Endoscopic ultrasonography (EUS) with radial scanning is an efficient diagnostic tool where there is suspicion of common bile duct (CBD) stones. Little is known about the use of linear EUS in this condition. The aim of this study was to evaluate the diagnostic efficiency of linear EUS in a large group of patients suspected to have bile duct stones, using endoscopic retrograde cholangiopancreatography (ERCP) with endoscopic sphincterotomy and exploration of the CBD using a Dormia basket, or surgical choledochotomy with choledochoscopy, as diagnostic "gold standards". Patients and methods: 134 patients with clinical suspecion of CBD stones were included in the study and prospectively evaluated, using EUS, and ERCP with endoscopic sphincteroctomy (127 patients), or choledochotomy with choledochoscopy where ERCP was unsuccessful (seven patients). EUS was done before ERCP using an echo endoscope (Pentax FG 32 UA; 5 - 7.5 MHz) and Hitachi EUB 405 ultrasound machine. ERCP was done using the TFJ 100 or TJ 20 Olympus duodenoscope. ERCP was carried out within a mean of 2 days after EUS. The longest time between EUS and ERCP was 3 days. The examiners were binded to the results of the other method used. Results: CBD stones were found in 91 (68 p.c.) patients at ERCP with ES or at surgery. The correct diagnosis was established by EUS in 85 patients. The remaining 43 patients without CBD stones were correctly diagnosed in 41 cases by means of EUS, giving an accuracy of 94 p.c., specificity of 93 p.c., a positive predictive value of 98 p.c., a negative predictive value of 87 p.c., and a Youden's index of 98 p.c....


    40/355

    Tytuł oryginału: The search for optimal treatment in relapsed and refractory acute myeloid leukemia.
    Autorzy: Robak Tadeusz, Wrzesień-Kuś Agata
    Źródło: Leuk. Lymphoma 2002: 43 (2) s.281-291, tab., bibliogr. 116 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • farmacja
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Despite the significant progress in the treatment of AML during the last 5-10 years, 20-40 p.c. of patients still do not achieve remission with standard induction therapy. In addition, 50-70 p.c. of patients in CR are likely to relapse. A major limitation of successful AML therapy is intrinsic or acquired drug resistance. Several pharmacological inhibitors of mechanisms inducing chemoresistance in leukemic cells have been investigated. New cytotoxic drugs, agents with novel mechanisms of action, and new treatment strategies are currently being investigated. The management of refractory or relapsed AML patients is reviewed in this study.


    41/355

    Tytuł oryginału: In vitro drug resistance profiles of adult acute lymphoblastic leukemia: possible explanation for difference in outcome to similar therapeutic regimens.
    Autorzy: Styczyński Jan, Wysocki Mariusz
    Źródło: Leuk. Lymphoma 2002: 43 (2) s.301-307, il., tab., bibliogr. 79 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • pediatria
  • farmacja

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Age has important prognostic impact in acute lymphoblastic leukemia (ALL). Adults with ALL have a worse prognosis compared to children. This may be due to different, unfavorable biology, poor treatment tolerance, drug resistance, higher expression of drug resistance related proteins. The lymphoblasts from adult ALL show an increased in vitro resistance to cytotoxic drugs, including prednisolone, dexamethasone, cytosine arabinoside, daunorubicin, L-asparaginase and methotrexate. Glucocorticoid resistance may be a fundamental difference between children, adolescents and adults with ALL, which may underlie different biological aspects and also explain the difference in prognosis. It seems that in vitro resistance to prednisolone with respect to the age might be a continuous variable in ALL patients, except infants. The greater the age, the higher the in vitro resistance to prednisolone. This may be due to induction of various defense mechanisms, such as an activation of P-glycoprotien, which develops throughout the life and protect the human against xenobiotics. Among a number of various drug resistance mechanisms, only several weak differences between adults and children with ALL have been reported including higher P-glycoprotein expression, lower methotrexate polyglutamate accumulation and possibly more often p53 gene mutations in adults. Intrinsic resistance, induction of drug resistance proteins expression during chemotherapy and co-existence of various mechanisms are common phenomena in adult ALL. It seems that age itself, more than drug resitance profile, reflects factors which have direct effect on chemotherapy response in adult ALL.


    42/355

    Tytuł oryginału: G-CSF administered in time-sequenced setting during remission induction and consolidation therapy of adult acute lymphoblastic leukemia has beneficial influence on early recovery and possibly improves long-term outcome: a randomized multicenter study.
    Autorzy: Hołowiecki Jerzy, Giebel Sebastian, Krzemień Sławomira, Krawczyk-Kuliś Małgorzata, Jagoda Krystyna, Kopera Małgorzata, Hołowiecka Beata, Grosicki Sebastian, Hellmann Andrzej, Dmoszyńska Anna, Paluszewska Monika, Robak Tadeusz, Konopka Lech, Maj Stanisław, Wojnar Jerzy, Wojciechowska Maria, Skotnicki Aleksander, Baran Wojciech, Cioch Maria
    Źródło: Leuk. Lymphoma 2002: 43 (2) s.315-325, il., tab., bibliogr. 25 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • farmacja
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Sixty-four untreated adult acute lymphoblastic leukemia (ALL) patients were randomized to receive chemotherapy alone, n = 31 or chemotherapy and granulocyte colony stimulating factor (G-CSF), n = 33. During induction patients received G-CSF for 5 days between four weekly Epirubicin + Vcradministrations, starting 36 h after each application and finishing 48 h before the next one with the intention to possibly generate a cell cycle dependent protection of normal hematopoietic progenitors and to stimulate granulopoiesis. The complete remission (CR) rate equaled 94 p.c. in the G-CSF group and 87 p.c in controls. Patients who received G-CSF, if compared to the controls, had shorter granulocytopenia during induction and consolidation, displayed a lower infection rate, completed the induction-consolidation quicker and stayed shorter in hospital during induction, p 0.001 - 0.04. Follow-up at 2 years revealed a rather higher probability of survival (59 vs. 27 p.c., p = 0.04) and a lower relapse rate (32 vs. 60 p.c.) in G-CSF arm than in controls. The beneficial influence of G-CSF administered in time-sequenced fashion on survival needs further confirmation.


    43/355

    Tytuł oryginału: Fludaraibine therapy in Waldenstr”m's macroglobulinemia patients treated previously with 2-chlorodeoxyadenosine.
    Autorzy: Lewandowski Krzysztof, Halaburda Kazimierz, Hellmann Andrzej
    Źródło: Leuk. Lymphoma 2002: 43 (2) s.361-363, il., tab., bibliogr. 10 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • farmacja
  • hematologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Six patients with Waldenstr”m's Macroglobulinemia (WM) resistant to previous 2-chlorodeoxyadenosine (2-CdA) therapy were treated with fludarabine. Both initial and subsequent therapy was administered according to the most widely used protocols. The median number of 2-CdA cycles given to the patients was 3.7 (range 2-5), while fludarabine was 1.7 (range 1-2). As a result partial remission was achieved in two patients and stabilization of the disease in the remaining four in spite of the inadequate response or progressive disease after 2-CdA therapy. Median monoclonal IgM concentration fell from 29.4 (range 11.6-37.1) to 16.4 g/l (range 3.8-29.1). At the same time hemoglobin concentration increased from median 114 (range 89-129) to 122 g/l (range 97-133). Side effects consisted of neutropenia (three patients) and thrombocytopenia (two patients). Two patients are alive with progression of the disease noted after 12 and 21 months of observation. One patients died as the result of cardiac disease, two other died of infection 5 and 16 months after cessation of therapy in the course of disease progression. The remaining patient was lost to follow-up after 10 months of observation with continuous stabilization. Our observation suggest that in WM patients resistant to 2-CdA, stabilization of the disease or even remission can be obtained with fludarabine.


    44/355

    Tytuł oryginału: Production of proangiogenic cytokines during thalidomide treatment of multiple myeloma.
    Autorzy: Dmoszyńska Anna, Bojarska-Junak Agnieszka, Domański Damian, Roliński Jacek, Hus Marek, Soroka-Wojtaszko Maria
    Źródło: Leuk. Lymphoma 2002: 43 (2) s.401-406, il., tab., bibliogr. 26 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • farmacja
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Recently a growing number of studies have suggested the efficacy of thalidomide (THAL) in the treatment of relapsed or resistant multiple myeloma. Some of these studies indicate that the thalidomide antimyeloma effect is associated with decreased vessel density. Here we first present our experience with THAL treatment and then focus on the determination of the role of proangiogenic cytokines during THAL therapy. Thirty relapsing or resistant multiple myeloma (MM) patients were treated with THAL at a median dose of 400 mg/daily. Eighteen responded to THAL therapy and 12 were resistant or intolerant to THAL. We determined the plasma level of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) as the main biological parameters associated with tumour angiogenesis. In addition I1-6 and TNFŕ levels were also assayed. Assessment of peripheral blood (PB) and bone marrow (BM) cytokine levels were done before and during THAL treatment at weeks 4 and 8 of therapy. In the responder group VEGF, bFGF I1-6 and TNFŕ concentrations were significantly decreased after four weeks of therapy both in PB and BM. In the non-responder group no significant changes in bFGF and VEGF levels were observed. However, a significant increase in IL-6 and TNF concentrations was evident. We conclude that the significant decrease of VEGF, bFGF, I1-6 and TNFŕ concentrations reflected response to THAL therapy. Also it seems that VEGF is a better marker of response to treatment than bFGF.


    45/355

    Tytuł oryginału: Influence of the alpha-1-adrenergic receptor blocker doxazosin on exercise-induced hyperkalemia in hemodialysis patients.
    Autorzy: Nowicki Michał, Waluś Tomasz, Kokot Franciszek
    Źródło: Kidney Blood Press. Res. 2002: 25 (1) s.55-60, il., tab., bibliogr. 25 poz.
    Sygnatura GBL: 313,314

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Background/Aim: Hyperkalemic responses to both physical exercise and ŕ-adreenergic stimulation are enhanced in patients with terminal renal failure. ŕ-Adrenergic blockade was found to protect against hyperkalemia during vigorous exercise in healthy men. The aim of the study was to examine the effects of the ŕ-adrenergic blocker doxazosin on exercise-induced hyperkalemia in hemodialysis patients. Methods: In a randomized, placebo-controlled, crossover design study, 15 anuric, chronic hemodialysis patietns were included. Doxazosin or placebo was given in a random order for 4 days before exercise. At the end of each phase of the study, a 30-min treadmill exercise test with a constant workload of 2 metabolic equivalents was performed followed by a 30-min recovery period. Results: The patients achieved 64 ń 3 and 62 ń 3 p.c. of maximal heart rate during the exercise test on doxazosin and placebo, respectively. The baseline plasma concentration of potassium was similar both on active treatment and on placebo (5.1 ń 0.2 mmol/l on doxazosin and 4.9 ń 0.1 mmol/l on placebo). The serum potassium concentration increased significantly and to a similar extent during the tests. The mean rates of potassium increment during exercise were 8.4 ń 1.5 ćmol/l/min on doxazosin and 6.9 ń 1.3 ćmol/l/min on placebo. During the recovery period, the serum potassium concentration significantly decreased in both arms of the study. There were no significant changes in plasma sodium, calcium, and phosphate levels during the tests. Hydrogen ion concentration in blood, serum insulin and glucose, and plasma aldosterone and renin activity were similar before the exercise tests...


    46/355

    Tytuł oryginału: Characterization of histamine H2-like receptors in duck cerebral cortical membranes by [3H]tiotidine binding.
    Autorzy: Zawilska Jolanta, Zalewska-Kaszubska Jadwiga, Przybysz Marzena, Nowak Jerzy Z.
    Źródło: Neurosci. Lett. 2002: 319 (3) s.149-152, il., bibliogr. 18 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • ptaki
  • in vitro

    Streszczenie angielskie: A selective (according to mammalian criteria) histamine (HA) H2-receptor radioligand ([3H]TIOT) was used to characterize HA receptors in duck cerebral cortex by an in vitro binding technique. The specific binding of [3H]TIOT to duck cerebral cortical membranes was found to be rapid, stable, saturable, reversible, and of high affinity. Saturation analysis resulted in a linear Scatchard plot suggesting binding to a single class of receptor binding sites with high affinity (Kd = 19.5 nM) and high capacity (Bmax - 356 fmol/mg protein). Competition studies showed the following relative rank order of potency of various HA receptor ligands to inhibit the [3H]TIOT binding: antagonists, tiotidine rantidine izolantidine ň cimetidine mepyramine thioperamide; agonists, HA ň 4-methylHA 2-methylHA dimaprit Rŕ-methylIHA. The biphasic nature of the displacement curve for HA and the effect of 5'-guanylimidodiphosphate indicate with [3H]TIOT represent either avian-specific H2-like HA receptors or a novel subtype of HA receptors, coupled to a signalling athway other than the adenylyl cyclase/cyclic adenosine monophosphate one.


    47/355

    Tytuł oryginału: New stereocontrolled synthesis and biological evaluation of 5-(1'-Hydroxyalkyl)-3-methylidenetetrahydro-2-furanones as potential cytotoxic agents.
    Autorzy: Janecki Tomasz, Błaszczyk Edyta, Studzian Kazimierz, Różalski Marek, Krajewska Urszula, Janecka Anna
    Źródło: J. Med. Chem. 2002: 45 (5) s.1142-1145, il., tab., bibliogr. 23 poz.
    Sygnatura GBL: 312,133

    Hasła klasyfikacyjne GBL:
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: A series of 3-methylidenetetrahydro-2-furanones 7 bearing various hydroxyalkyl substituents in position 5 were synthesized using novel diastereo- and enantioselective methodology. In vitro cytotoxicity data demonstrated that all prepared compounds were active against L-1210 and HL-60 tissue culture cells with 7e being the most potent (IC50 = 6.9 ćM). Also an increase in activity with an increase in lipophilicity of the substituents in the order H , alkyl phenyl was observed.


    48/355

    Tytuł oryginału: Expression of mutant thyroid hormone nuclear receptors is associated with human renal clear cell carcinoma.
    Autorzy: Kamiya Yuji, Puzianowska-Kuźnicka Monika, McPhie Peter, Nauman Janusz, Cheng Sheue-yann, Nauman Alicja
    Źródło: Carcinogenesis 2002: 23 (1) s.25-33, il., tab., bibliogr. 59 poz.
    Sygnatura GBL: 312,779

    Hasła klasyfikacyjne GBL:
  • nefrologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Thyroid hormone (T3) regulates proliferation and differentiation of cells, via its nuclear receptors (TRs). These processes have been shown to be abnormally regulated during carcinogenesis. We have previously found aberrant expression of TRŕ and TRá mRNAs in renal clear cell carcinoma (RCCC), suggesting possible involvement of TRs in the carcinogenesis of RCCC. To understand the molecular actions of TRs in RCCC, cDNAs for TRá1 and TRŕ1 were cloned from 22 RCCC tissues and 20 surrounding normal tissues. Mutations were found in seven TRá1 and three TRŕ1 cDNAs. Two TRá1 cDNAs had a single mutation, while five TRá1 and three TRŕ1 had two or three mutations. Most of the mutations were localized in the hormone-binding domain. Using the TRs prepared by in vitro transcription/translation, we found that these mutations led to a loss of T3 binding activity and/or impairment in binding to thyroid hormone response elements (TREs). Furthermore, nuclear extracts from RCCC tissues also exhibited impairment in binding to TREs. These results indicate that the normal functions of TRs in RCCC tissues were impaired. Together with the aberrant expression patterns, these mutated TRs could contribute to the carcinogenesis of RCCC.


    49/355

    Tytuł oryginału: The adipose tissue - a novel endocrine organ of interest to the nephrologist.
    Autorzy: Więcek Andrzej, Kokot Franciszek, Chudek Jerzy, Adamczak Marcin
    Źródło: Nephrol. Dial. Transplant. 2002: 17 (2) s.191-195, il., bibliogr. 39 poz.
    Sygnatura GBL: 312,872

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • nefrologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    50/355

    Tytuł oryginału: Efficacy of the new long-acting formulation of lanreotide (Lanreotide Autogel) in the management of acromegaly.
    Autorzy: Caron Ph., Beckers A., Cullen D. R., Goth M. I., Gutt B., Laurberg P., Pico A. M., Valimaki M., Zgliczyński W.
    Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (1) s.99-104, il., tab., bibliogr. 22 poz.
    Sygnatura GBL: 310,620

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • farmacja

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Lanreotide Autogel is a new long-acting aqueous preparation of lanreotide for the treatment of acromegaly and is administered by deep sc injection from a small volume, prefilled syringe. The aim of this study was to evaluate the efficacy and safety of this new ong-acting formulation in a large population of acromegalic patients previously responsive to lanreotide 30 mg, im (substained release microparticle formulation). Lanreotide Autogel was administered by deep sc injection every 28 d to 107 patients (54 males and 53 females; mean age, 54 ń 1.2 yr). All patients had been treated with lanreotide (30 mg) for at least 3 months before study entry and had a mean GH level less than 10 ng/ml after at least 4 subsequent im injections every 14 d (48 p.c.), 10 d (32 p.c.), or 7 d (20 p.c.). Treatment was switched from lanreotide 30 mg injected every 14, 10, or 7 d to 60, 90, or 120 mg lanreotide Autogel, respectively, every 28 d. After three fixed dose injections of lanreotide Autogel, mean lanreotide levels were similar to those obtained at steady state with lanreotide 30 mg. During lanreotide Autogel treatment, the control of acromegalic symptoms was comparable with that previoulsy achived during lanreotide 30 mg treatment. After 3 injections of lanreotide Autogel, mean GH (2.87 ń 0.22 ng/ml) and IGF-I (317 ń 15 ng/ml) values were comparable with those recorded at the end of lanreotide 30 mg treatment (GH, 2.82 ń 0.19 ng/ml; IGF-I, 323 ń 16 ng/ml). GH levels below 2.5 ng/ml and age-/sex-normalized IGF-I were achived in 33 p.c. and 39 p.c. of patients during lanreotide 30 mg and lanreotide Autogel treatment, respectively...


    51/355

    Tytuł oryginału: Surgical view of the treatment of patients with hepatoblastoma. Results from the First Prospective Trial of the International Society of Pediatric Oncology Liver Tumor Study Group (SIOPEL-1).
    Autorzy: Schnater J. Marco, Aronson Dani‰l C., Plaschkes Jack, Perilongo Giorgio, Brown Julia, Otte Jean-Bernard, Brugieres Laurences, Czauderna Piotr, MacKinlay Gordon, Vos Anton
    Źródło: Cancer 2002: 94 (4) s.1111-1120, il., tab., bibliogr. 20 poz.
    Sygnatura GBL: 311,015

    Hasła klasyfikacyjne GBL:
  • pediatria
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Background. Surgical resection is the cornerstone of treatment for patients with hepatoblastoma (HB). The Society of Pediatric Oncology Liver Tumor Study Group launched its first prospective trial (SIOPEL-1) with the intention to treat all patients with preoperative chemotherapy and delayed surgical resection. The objective of this article was to assess the assumed surgical advantages of primary chemotherapy. Methods. Between 1990 and 1994, 154 patients age 16 years with HB were registered on SIOPEL-1. The pretreatment extent of disease was assessed, and, after undergoing biopsy, patients were treated with cisplatin 80 mg/mý intravenously over 24 hours and doxorubicin 60 mg/mý intravenously over 48 hours by continuous infusion (PLADO). Generally, tumors were resected after four of a total of six courses of PLADO. Results. One hundred twenty eight patients underwent surgical resection (13 patients underwent primary surgery, and 115 patients underwent delayed surgery after PLADO). A pretreatment surgical biopsy was performed in 96 of 128 patients (75 p.c.). Biopsy complications occurred in 7 of 96 patients (7 p.c.). Twenty-two patients showed pulmonary metastases at the time of diagnosis, and 7 patients underwent thoracotomy. Operative morbidity and mortality were 18 p.c. and 5 p.c., respectively. Complete macroscopic surgical resection was achieved in 106 patients (92 p.c.), including 6 patients who underwent orthotopic liver transplantation. The actuarial 5-year event free survival (EFS) rate for all 154 patients in the study was 66 p.c., and the overall survival (OS) rate was (75 p.c.)...


    52/355

    Tytuł oryginału: Elevated soluble intercellular adhesion molecule-1 levels in obesity: relationship to insulin resistance and tumor necrosis factor-ŕ system activity.
    Autorzy: Strączkowski Marek, Lewczuk Piotr, Dzienis-Strączkowska Stella, Kowalska Irina, Stępień Agnieszka, Kinalska Ida
    Źródło: Metabolism 2002: 51 (1) s.75-78, il., tab., bibliogr. 29 poz.
    Sygnatura GBL: 303,583

    Hasła klasyfikacyjne GBL:
  • immunologia
  • endokrynologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Intercellular adhesion molecule-1 (ICAM-1) is 1 of the possible factors linking obesity and diabetes with cardiovascular disease, however, the mechanism of the increase in ICAM-1 concentration in obesity remains unclear. Therefore, the aim of the present study was to assess plasma soluble ICAM-1 (sICAM-1) levels on obese subjects with normal glucose tolerance and to evaluate whether those levels may be related to insulin resistance and tumor necrosis factor-ŕ (TNFŕ) system activity. The study was performed in 8 lean and 15 obese subjects. Anthropometric and biochemical parameters were measured, and insulin sensitivity was evaluated using the euglycemic hyperinsulinemic clamp technique (insulin infusion, 50 mU x kg**-1 x h**-1). Obese subjects were markedly more hyperinsulinemic and insulin resistant and had higher plasma soluble TNF receptor 2 (sTNFR2) and sICAM-1 levels. sICAM-1 was related positively to body mass index (BMI), waist-to-hip ratio (WHR), percent of body fat, glycated hemoglobin (HbA1c), plasma insulin and triglycerides (TG), TNFŕ, and sTNFR2 and negatively to insulin sensitivity. Multiple regression analysis showed that only sTNFR2 and insulin sensitivity were independent predictors of sICAM-1 concentrations and were responsible for 66 p.c. of sICAM-1 variability. We conclude that an increase in plasma sICAM-1 concentration in obesity is related to TNFŕ system activation and insulin resistance.


    53/355

    Tytuł oryginału: Enhanced T cells interactions with extracellular matrix proteins in infertile women with endometriosis.
    Autorzy: Jerzak Małgorzata, Baranowski Włodzimierz, Rechberger Tomasz, Górski Andrzej
    Źródło: Immunol. Lett. 2002: 81 (1) s.65-70, il., tab., bibliogr. 53 poz.
    Sygnatura GBL: 312,663

    Hasła klasyfikacyjne GBL:
  • immunologia
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Essential features of endometriosis involve interactions with extracellular matrix (ECM). Recent data emphasize the important role of ECM proteins in the regulation of T cell function. The aim of this study was to determine activated T cell adhesion to ECM proteins in infertile women with endometriosis. Nine women with endometriosis diagnosed by laparoscopy according to the Revised American Fertility Society classification and ten normal healthy women with a previous successful pregnancy outcome were studied. We investigated phorbol acetate myristate (PMA) or phytohemaglutinin (PHA) activated peripheral blood T cell adhesion to the following proteins of ECM: collagen IV (C-IV), elastin (E) and fibronectin (Fn). In addition, CD4, CD8, CD29, CD45RO exspression on peripheral CD3+ T cells were studied using flow cytometry. We determine that PHA-activated T cell adhesion to C-IV and Fn are significantly higher in infertile women with endometriosis when compared to normal healthy women (P 0.05). No significant differences were noted in T cell's surface antigens expression between study groups. Our data suggest the existence of disturbed T cell-ECM interactions in infertile women with endometriosis. Further studies are needed to determine the role of these abnormalities in the pathogenesis of endometriosis.


    54/355

    Tytuł oryginału: Allelic distribution of complement components BF, C4A, C4B, and C3 in Psoriasis vulgaris.
    Autorzy: Cisło Maria, Hałasa Jolanta, Wąsik Feliks, Nockowski Piotr, Prussak Magdalena, Mańczak Maria, Kuśnierczyk Piotr
    Źródło: Immunol. Lett. 2002: 80 (3) s.145-149, tab., bibliogr. 58 poz.
    Sygnatura GBL: 312,663

    Hasła klasyfikacyjne GBL:
  • genetyka
  • immunologia
  • dermatologia i wenerologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Psoriasis vulgaris is a multifactorial disease; the strongest association was established with the HLA complex. The actual disease-predisposing gene(s) has not been identified yet, but several genes from this region were examined in addition to HLA-C and -B. However, HLA-linked complement component polymorphic genes were not extensively studied. Therefore, we typed 67 psoriatic patients for alleles of the HLA-linked complement components BF, C4A and C4B. Alleles of C3, encoded on another chromosome, were established in parallel as a negative control. Frequencies in patients were compared with thise in unrelated healthy controls, 100 individuals for C4A and C4B, 890 for BF and 4719 for C3. We found no association of BF alleles with disease, similarly to C3. Among C4 alleles, C4B*3 was present in 13,4 p.c. of patients as compared with 1 p.c. of controls (OR, 15.36; 95 p.c. CI, 1.897 - 124.42; P = 0.0009), and C4A*6 was present in 19.4 p.c. of patients versus 7 p.c. of controls (OR, 3.20; 95 p.c. CI, 1.202 - 8.508; P = 0.0155). The high frequency of C4B*3 in psoriatics has not been described so far. These results suggest a contribution of C4 genes themselves or a closely linked gene to the susceptibility to psoriasis.


    55/355

    Tytuł oryginału: Endothelin in the mechanism of endothelial injury and neutrophil adhesion in the post-ischemic guinea-pig heart.
    Autorzy: Kurzelewski Michał, Czarnowska Elżbieta, Beręsewicz Andrzej
    Źródło: Eur. J. Pharmacol. 2002: 434 (1/2) s.95-107, il., tab., bibliogr. s. 105-107
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • Świnki morskie

    Streszczenie angielskie: This study addressed the hypothesis that endothelin promotes neutrophil accumulation in ischemic/reperfused myocardium, not only via its direct effect on neutrophils, but also because it mediates post-ischemic endothelial injury. Langendorff-perfussed guinea-pig hearts were subjected to 30 min ischemia/35 min reperfusion, and infusion of neutrophils between 15 and 25 min of reperfusion. The infusion of the endothelin ETA/ETB receptor antagonist, tezosentan, the endothelin ETA receptor antagonist, BQ 123 [cyclo(-D-Trp-D-Asp-Pro-D-Val-Leu-], and superoxide dismutase was terminated at reperfusions, 5 min before the start of the neutrophil infusion, to avoid the contact of the drugs with neutrophils. Coronary flow responses to acetylcholine and nitroprusside were used as measures of endothelium-dependent and -independent vascular function, respectively. Neutrophil adhesion and endothelium glycocalyx ultrastructure were assessed in histological preparations. Ischemia/reperfusion resulted in a 54 p.c.-impaired acetylcholine response, endothelium glycocalyx disruption, and enhanced neutrophil adhesion (21.6 p.c. of microvessels contained neutrophils vs. 2.6 p.c. in sham group), the latter prevented by a selectin blocker, sulfatide, 20 ćg/ml. These alterations were completely prevented by 0.5 and 5 nM, but not 0.05 nM, tezosentan, and were greatly attenuated by BQ 123, 1 and 10 nM. The glycocalyx-protective effect of these interventions preceded their effect on neutrophil adhesion. Superoxide dismutase, 150 IU/ml, reported before by us to protect post-ischemic endothelium glycocalyx, here prevented the post-ischemic endothelial dysfunction and neutrophil adhesion...


    56/355

    Tytuł oryginału: Successful use of activated recombinant factor VII to control bleeding abnormalities in a patient with a left ventricular assist device.
    Autorzy: Ziętkiewicz Mirosław, Garlicki Mirosław, Domagała Jerzy, Wierzbicki Karol, Drwiła Rafał, Pfitzner Roman, Dziatkowiak Antoni
    Źródło: J. Thorac. Cardiovasc. Surg. 2002: 123 (2) s.384-385, bibliogr. 5 poz.
    Sygnatura GBL: 310,230

    Hasła klasyfikacyjne GBL:
  • hematologia
  • kardiologia

    Typ dokumentu:
  • praca kazuistyczna
  • komunikat
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć męska


    57/355

    Tytuł oryginału: N-acetyltransferase 2 polymorphism in sporadic Parkinson's disease in a Polish population.
    Autorzy: Białecka Monika, Gawrońska-Szklarz Barbara, Droździk Marek, Honczarenko Krystyna, Stankiewicz Jan
    Źródło: Eur. J. Clin. Pharmacol. 2002: 57 (12) s.857-862, il., tab., bibliogr. 28 poz.
    Sygnatura GBL: 312,148

    Hasła klasyfikacyjne GBL:
  • genetyka
  • neurologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Objective: A genetic background of Parkinson's disease has been suggested, including genes implicated in xenobiotic metabolism. So far, many candidate genes responsible for the occurrence of the disease have been enumerated. This study was carried out to determine the presence of N-acetyltransferase 2 polymorphism in Parkinson's disease patients in a Polish population. Methods: Fifty-four patients with diagnosed sporadic Parkinson's disease and 81 healthy individuals were enrolled into the study. The N-acetyltransferase 2 alleles (*4, *5, *6 and *7) were identified using polymerase chain reaction-restriction fragment length polymorphism methods with DNA extracted from peripheral blood. Results: A preponderance of slow acetylators in patients with Parkinson's disease was demonstrated. Among 54 subjects with parkinsonism, 64.8 p.c. were homozygous for two mutated alleles responsible for the slow-acetylator phenotype. In the control group, a predominance of fast acetylators was noted. Subjects homozygous and heterozygous with genotypes determining fast acetylation constituting 53 p.c. of subjects, whereas 47 p.c. were slow acetylators. Comparison of the two groups of the study, i.e. Parkinson's disease and healthy individuals, revealed a statistically significant predominance of slow acetylators in Parkinson's disease patients (P 0.05). The risk of Parkinson's disease development was more than two times greater in slow acetylators than healthy subjects. The frequency of point mutations was similar both in patients with Parkinson's disease and the healthy cotrols. Conclusion: Slow-acetylation genotype may be an important factor of individual susceptibility to Parkinson's disease.


    58/355

    Tytuł oryginału: Serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in different histological variants of rheumatoid synovitis.
    Autorzy: Klimiuk P. A., Sierakowski S[tanisław], Latosiewicz R[obert], Cylwik B[ohdan], Skowroński J[an], Chwiecko J.
    Źródło: Rheumatology 2002: 41 (1) s.78-87, il., tab., bibliogr. 50 poz.
    Sygnatura GBL: 303,271

    Hasła klasyfikacyjne GBL:
  • reumatologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective. Rheumatoid synovitis is characterized by an invasive and tissue-destructive infiltrate of lymphocytes, macrophages and synoviocytes. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) produced by these cells are important in the remodelling of the articular tissue in rheumatoid arthritis (RA). The aim of this study was to explore whether the serum concentrations of MMPs and their inhibitors were correlated with the histological appearance of the disease. Methods. Tissue and serum samples were obtained from 37 patients with clinically active RA and 30 with osteoarthritis (OA). Morphological analysis allowed the division of RA synovial specimens into two distinct types. In 22 samples only diffuse infiltrates of mononuclear cells without further microanatomical organization were found. In 15 specimens we observed lymphocytic conglomerates with germinal centre-like structures. Serum concentrations of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), gelatinase B (MMP-9), TIMP-1 and TIMP-2 were measured with an ELISA technique. Results. Unique serum profiles of MMPs and TIMPs were identified in each of the two histological types of RA synovitis. The serum concentrations of MMP-1, MMP-3 and MMP-9 were higher in RA patients than in OA patients used as a control group (P 0.001 for all comparisons). These three MMPs dominated in the serum of RA patients with follicular synovitis compared with those diffuse synovitis (P 0.05, P 0.01 and P 0.001 respectively)...


    59/355

    Tytuł oryginału: GLP-1 as a satiety fctor in children with eating disorders.
    Autorzy: Tomasik P. J., Sztefko K., Malek A.
    Źródło: Horm. Metab. Res. 2002: 34 (2) s.77-80, il., tab., bibliogr. 24 poz.
    Sygnatura GBL: 312,226

    Hasła klasyfikacyjne GBL:
  • endokrynologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • płeć żeńska

    Streszczenie angielskie: GLP-1, with its insulinotropic properties and direct action on satiety center in the brain, may be the main hormone regulating the amount of ingested food. In this study, GLP-1 secretion was investigated in age-matched adolescent girls (14 ń 2 years): 13 with anorexia nervosa (BMI 14.8 ń 1.4 kg/mý), 13 with simple obesity (BMI 33.0 ń 3.3 kg/mý) and 10 healthy girls as a control group (BMI 21.6 ń 0.7 kg/mý). Each girl was subjected to OGTT and standard meal tests after a 12h overnight fast. Blood samples were collected before and 15, 30, 60, and 120 min after the stimulation. The mean fasted GLP-1 levels in simple obesity group (1.6 ń 0.3 pmol/l) and in anorexia nervosa group (1.7 ń 0.3 pmol/l) were significantly lower than those in the control group (2.6 ń 0.4 pmol) (p 0.05) in both cases). The highest peak concentration of GLP-1 was observed in the control group after both stimuli. In each group, the mean integrated GLP-1 outputs were almost twice as high after OGTT than after the test meal (p 0.001 in each case). In our opinion, low secretion of GLP-1 in girls with simple obesity may seriously and negatively influence the course of this disease. On the other hand, low GLP-1 levels in girls with anorexia nervosa are beneficial and promote appetite.


    60/355

    Tytuł oryginału: Increased nitric oxide bioavailability in endothelial cells contributes to the pleiotropic effect of cerivastatin.
    Autorzy: Kalinowski Leszek, Dobrucki Lawrence W., Brovkovych Viktor, Maliński Tadeusz
    Źródło: Circulation 2002: 105 (8) s.933-938, il., bibliogr. 17 poz.
    Sygnatura GBL: 311,033

    Hasła klasyfikacyjne GBL:
  • farmacja
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Although statins preserve endothelial function by reducing serum cholesterol levels, it has been suggested they may also stimulate nitric oxide (NO) synthase in endothelium with concurrent increase in superoxide (O2-) generation, leading to impairment of NO activity. Therefore, measurements of biologically active NO and O2 - in endothelium after exposure to the HMG-CoA reductase inhibitor cerivastatin were undertaken to evaluate its potential effect on NO biological activity. Methods and Results - Highly sensitive electrochemical NO and O2 - microsensors were placed near the surface of a single human umbilical vein endothelial cell, and the kinetics of NO and O2 - release were recorded in vitro. Cerivastatin demonstrated a time-dependent effect on NO release in endothelial cels. The initial release (approximately the first 3 minutes) was concentration-dependent (0.01 to 10 ćmol/L) and was similar to that observed for typical NO synthase agonists calcium ionophore or acetylcholine. Cerivastatin stimulated NO release at a favorable rate and scavenged O2-, which led to the preservation of the active concentration of NO. The sustained effect (after ÷ 6 hours) of cerivastatin on endothelium was associated with an ÷ 35 p.c. increase in NO release as compared with the initial effect. In contrast to the initial effect, the sustained effect of cerivastatin was shown at concenrations ÷ 100-fold lower and was dependent on inhibition of endothelial HMG-CoA reductase. Conclusions - These data provide direct evidence to prove that in the presence of cerivastatin, the NOS system in endothelium operates with high efficiency toward increasing NO activity by activation of NO release and by concurrent inactivation of O2-.


    61/355

    Tytuł oryginału: Juvenile idiopathic arthritis (JIA) is primarily associated with HLA-DR8 but not DQ4 on the DR8-DQ4 haplotype.
    Autorzy: Smerdel A., Ploski R., Flato B., Musiej-Nowakowska E., Thorsby E., Forre O.
    Źródło: Ann. Rheum. Dis. 2002: 61 (4) s.354-357, tab., bibliogr. 17 poz.
    Sygnatura GBL: 310,264

    Hasła klasyfikacyjne GBL:
  • immunologia
  • reumatologia
  • genetyka

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background: Juvenile idiopathic arthritis (JIA) is strongly associated with the DR8-DQ4 haplotype. The genes encoding DR8 and DQ4 are in strong linkage disequilibrium (LD) and oocur together on the same HLA haplotype in almost all patients and controls. Because of the strong LD it is not clear whether DR8, DQ4, or both, are primarily associated with JIA. Objective: To unveil the primary association of JIA - that is, with DR8 or DQ4. Methods: DRB1, DQA1, and DQB1 alleles of 585 Norwegian and 47 Polish unrelated patients with JIA (categorised as pauciarticular and rheumatoid factor negative polyarticular JIA), and of 3155 Norwegian and 158 Polish unrelated controls, were typed using a polymerase chain reaction or oligonucleotide hybridisation and sequence-specific primers method. Results: Several haplotypes which encoded DR8 (that is, carried DRB1*08) and which did not encode DQ4 (that is, did not carry DQA1*0401) were found. Such haplotypes were found in three Norwegian patients and two controls (p = 0.029). In the Polish population such haplotypes were found among four patients with JIA and two controls (p = 0.025). No haplotypes which carried DQA1*0401 and DQB1*0402 in the absence of DRB1*08 were found, either among patients with JIA (Polish and Norwegian) or among the controls (Polish). Conclusion: On the DR8-DQ4 haplotype the DRB1*08 allele is primarily associated with JIA.


    62/355

    Tytuł oryginału: Estrogen inhibits systemic T cell expression of TNF-ŕ and recruitment of TNF-ŕ+ T cells and macrophages into the CNS of mice developing experimental encephalomyelitis.
    Autorzy: Ito Atsushi, Buenafe Abigail C., Matejuk Agata, Zamora Alex, Silverman Marc, Dwyer Jami, Vandenbark Arthur A., Offner Halina
    Źródło: Clin. Immunol. 2002: 102 (3) s.275-282, il., tab., bibliogr. 40 poz.
    Sygnatura GBL: 305,911

    Hasła klasyfikacyjne GBL:
  • immunologia
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Estrogen treatment has been found to have suppressive activity in several models of autoimmunity. To investigate the mechanism of 17á-estradiol (E2) suppression of experimental autoimmune encephalomyelitis, we evaluated E2 effects on TNF-ŕ experssion in the central nervous system (CNS) and spleen of C57BL/6 mice immunized with MOG 35-55/CFA. Kinetic analysis demonstrated that E2 treatment drastically decreased the recruitment of total inflammatory cells as well as TNF-ŕ+ macrophages and T cell into the CNS at disease onset. In contrast, E2 had only moderate effects on the erlatively high constitutive TNF-ŕ expression by resident CNS microglial cells. E2 treatment also had profound inhibitory effects on expression of TNF-ŕ by splenic CD4+ T cells, including those responsive to MOG 35-55 peptide. We propose that the mechanism of E2 protection may involve both systemic inhibition of TNF-ŕ expression and local (CNS) ercruitment of inflammatory cels, with modest effects on TNF-ŕ expression by resident CNS microglial cells.


    63/355

    Tytuł oryginału: Extent of, and factors associated with, delay to hospital presentation in patients with acute coronary disease (the GRACE registry).
    Autorzy: Goldberg Robert J., Steg Philippe Gabriel, Sadiq Immad, Granger Christopher B., Jackson Elizabeth A., Budaj Andrzej, Brieger David, Avezum Alvaro, Goodman Shaun
    Źródło: Am. J. Cardiol. 2002: 89 (7) s.791-796, il., tab., bibliogr. 15 poz.
    Sygnatura GBL: 304,619

    Hasła klasyfikacyjne GBL:
  • organizacja ochrony zdrowia
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Our primary study aim was to examine extent of, and factors associated with, delay in seeking medical care in a large multinational registry of patients with acute myocardial infarction (AMI) and unstable angina pectoris. A secondary goal was to examine the relation between duration of prehospital delay and receipt and timing of coronary reperfusion strategies. Investigators from 14 countries are participating in the Global Registry of Acute Coronary Events (GRACE) project. The study sample consisted of 3,693 patients with ST-segment elevation AMI, 2,935 with non-ST-segment elevation AMI, and 3,954 patients with unstable angina hospitalized between 1999 and 2001. The average and median delay times were longest in patients with non-ST-segment elevation AMI (6.1 and 3.0 hours, respectively) followed by patients with unstable angina (5.6 and 3.0 hours) and those with ST-segment elevation AMI (4.7 and 2.3 hours). Approximately 41 p.c. of patients with ST-segment elevation AMI presented to the 94 study hospitals within 2 hours of the onset of acute coronary symptoms; the compared with approximately one third of patients with non-ST-segment elevation AMI and unstable angina. Several demographic and clinical characteristics were associated with prehospital delay. In patients with ST-segment elevation AMI, duration of prehospital delay was inversely related to the receipt of thrombolytic therapy, but was inconsistently related to the use pf percutaneous coronary interventions. The results of this study demonstrate that a large proportion of patients continue to exhibit prolonged delay in seeking medical care after the onset of acute coronary symptoms and remain in need of targeted educational efforts to reduce extent of delay.


    64/355

    Tytuł oryginału: Fine-needle aspiration biopsy of the thyroid in an area of endemic goitre: influence of restored sufficient iodine supplementation on the clinical significance of cytological results.
    Autorzy: Słowińska-Klencka Dorota, Klencki Mariusz, Sporny Stanisław, Lewiński Andrzej
    Źródło: Eur. J. Endocrinol. 2002: 146 (1) s.19-26, il., tab., bibliogr. 38 poz.
    Sygnatura GBL: 301,344

    Hasła klasyfikacyjne GBL:
  • pediatria
  • endokrynologia
  • farmacja
  • otorynolaryngologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Objective: The iodine status of the population of Poland has markedly improved over the past years. The aims of this paper were: (i) to examine the diagnostic, value of fine-needle aspiration biopsy (FNAB) of the thyroid in goitre endemic regions (in conditions of improved iodine supply), and (ii) to find whether the changes in iodine supply have already influenced the clinical interpretation of cytological results. Methods: Cytological diagnoses, based on 3782 aspirates, obtained from 3572 patients during the years 1985-1999, were verified by reference to the results of postoperative examinations. The relative occurrences of selected cytological results in 1992-1999 were also compared (patients not subjected to surgery were included). Results: We have found that the frequenty of neoplastic lesions significantly decreased throughout the examined period (P 0.02). The ratio of the follicular carcinoma frequency increased from 1.7 during 1992-1993 up to 8.0 during 1998-1999 (P 0.05). The frequency of cytologically diagnosed chronic thyroiditis increased from 1.5 p.c. in 1992 to 5.7 p.c. in 1999 (P 0.001); the percentage of cytological diagnosis of 'follicular neoplasm' decreased during the same time (P 0.001). The risk of malignancy significantly lowered in the cytological diagnoses of 'follicular neoplasm' from 15 p.c. during 1985-1993 to 6 p.c. during 1996-1999 (P 0.05). Conclusions: The diagnostic value of FNAM during the period without proper iodine prophylaxis did not differ significantly from that during the last examined period. However, the changes in iodine supply have markedly and promptly affected the clinical significance of particular cytological results.


    65/355

    Tytuł oryginału: Angiogenesis of endocrine gland tumours - new molecular targets in diagnostics and therapy.
    Autorzy: Stępień H. M., Kołomecki K., Pasieka Z., Komorowski J., Stępień T., Kuzdak K.
    Źródło: Eur. J. Endocrinol. 2002: 146 (2) s.143-151, tab., bibliogr. 96 poz.
    Sygnatura GBL: 301,344

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • onkologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Angiogenesis in one of the key stages in the development of neoplastic tumours, in which a small group of mutated cells transforms into a large malignant tumour metastasising to the neighbouring tissues and organs. The studies on the significance of neoangiogenesis in the progression of endocrine gland neoplasms have recently become one of the most rapidly evolving branches of molecular endocrinology. The induction of angiogenesis has been demonstrated to result from the imbalance between positive and negative factors which control this process. Our paper presents the results of current studies on the role of factors such as molecular markers of angiogenesis (e.g. vascular endothelial growth factor and basic fibroblast growth factor), metalloproteinases (which regulate the decomposition of the extracellular matrix) and their inhibitors, and adhesive molecules (e.g. soluble vascular cellular adhesion molecule-1 and soluble intracelular adhesion lecule-1) in the pathogenesis and diagnostics of endocrine gland tumours in humans. Also, we discuss new therapeutic strategies for inhibiting the growth of neoplasms by blocking the formation of blood vessels using angiogenesis antagonists, which inhibit various stages of angiogenesis. More and more data are being accumulated suggesting that these preparations could, in the near future, be used in the pharmacotherapy of some endocrine gland neoplasms.


    66/355

    Tytuł oryginału: O**6-Alkylguanine-DNA-alkyltransferase activity in peripheral leukocytes, smoking and risk of lung cancer.
    Autorzy: Boffetta Paolo, Nyberg Fredrik, Mukeria Anush, Benhamou Simone, Constantinescu Vali, Batura-Gabryel Halina, Brske-Hohlfeld Irene, Schmid Giovanni, Simonato Lorenzo, Pelkonen P„ivi, Hall Janet
    Źródło: Cancer Lett. 2002: 180 (1) s.33-39, tab., bibliogr. 25 poz.
    Sygnatura GBL: 306,023

    Hasła klasyfikacyjne GBL:
  • genetyka
  • pulmonologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • dorośli = 65 r.ż.

    Streszczenie angielskie: The level of activity of O**6-alkalguanine-DNA-alkytransferase (AGT), a DNA repair enzyme, in blood lymphocytes may be a marker of susceptibility to lung cancer. We measured the AGT activity level, expressed as plomes of repaired bases/mg protein, in leukocytes of 153 lung cancer cases (of whom 80 were never smokers) and 106 controls (76 never smokers) enrolled in eight centres from seven countries. Subjects were interviewed with respect to active smoking and exposure to environmental tobacco smoke (ETS). Among never smokers, the odds ratios (ORs) of lung cancer were 1.3 (95 p.c. confidence interval 0.5-3.9), 1.5 (0.6-4.1) and 1.4 (0.5-3.8) in quartiles of decreasing AGT activity level, as compared to the upper quartile (P value of test for linear trend 0.6). Corresponding ORs among smokers were 3.4 (0.9-13), 2.0 (0.5-8.3) and 0.4 (0.1-1.6) (P value of test for linear trend 0.4). No interaction was suggested between AGT activity level and either cumulative smoking or exposure to ETS. Reduced AGT activity was not clearly associated with increased lung cancer risk in either smokers or non-smokers. However, the small size of our study argues for a prudent interpretation of our results.


    67/355

    Tytuł oryginału: Contrast echocardiography and medical economics: looking into the crystal ball.
    Autorzy: Distante A., Dankowski R., Mincarone P., Leo C. G., Gianicolo E., Voci P., Morales M. A., Rovai D.
    Źródło: Eur. Heart J. 2002: 4 supl. C s.C39-C47, tab., bibliogr. 78 poz.
    Sygnatura GBL: 306,213

    Hasła klasyfikacyjne GBL:
  • ekonomika
  • kardiologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The past decade has witnessed the development of contrast echocardiography. This technique has demonstrated clinical utility in some applications and extremely promising results have been reported for others, although there are still several obstacles to its acceptance by regulatory authorities and routine users. In addition, the eficacy and cost-effectiveness must be proven against 'gold standard' techniques, which in several cases are just accepted standards. Furthermore, the competition is quite strong both between new techniques and with established ones, and all of the imaging modalities need to find their most appropriate role within this comples environment.


    68/355

    Tytuł oryginału: Plasma fibrinogen and troponin I in acute coronary syndrome and stable angina.
    Autorzy: Gil Maciej, Zarębiński Maciej, Adamus Jerzy
    Źródło: Int. J. Cardiol. 2002: 83 (1) s.43-46, il., tab., bibliogr. 10 poz.
    Sygnatura GBL: 305,693

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We aimed to determine whether there is a stratification among patients with different stages of coronary artery disease with respect to plasma fibrinogen levels, and to assess diagnostic value of plasma fibrinogen in comparison to troponin I in patients with acute coronary syndrome. Fifty-one consecutive patients presenting to our department with acute coronary syndrome within the last 24 h and 52 patients with stable angina with no episode of unstable disease within the last month were analysed. Forty-nine patients with acute coronary syndrome in which both troponin I and fibrinogen levels were prsent were further evaluated. Blood was collected on admission for routine laboratory tests. Statistical analysis was done using Student's t-test, Pearson correlation and chi-square test, P 0.05 being considered statistically significant. Plasma fibrinogen levels (g/l) were significantly higher in patients presenting with unstable than with stable angina (3.87 ń 1.2 vs. 3.26 ń 0.65 P = 0.002). We have found significant correlation between fibrinogen and troponin I levels in coexist and correlate. These results seem to confirm the role of this acute phase protein in the pathophysiology of acute coronary syndrome.


    69/355

    Tytuł oryginału: Anthrapyridones, a novel group of antitumour non-cross resistant anthraquinone analogues. Synthesis and molecular basis of the cytotoxic activity towards K562/DOX cells.
    Autorzy: Tarasiuk J., Stefańska B., Plodzich I., Tkaczyk-Gobis K., Seksek O., Martelli S., Garnier-Suillerot A., Borowski E.
    Źródło: Br. J. Pharmacol. 2002: 135 (6) s.1513-1523, il., tab., bibliogr. s. 1522-1523
    Sygnatura GBL: 301,182

    Hasła klasyfikacyjne GBL:
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: 1. Multidrug resistance (MDR) to antitumour agents, structurally dissimilar and having different intracellular targets, is the major problem in cancer therapy. MDR phenomenon is associated with the presence of membrane proteins which belong to the ATP-binding cassette family transporters responsible for the active drug efflux leading to the decreased intracellular accumulation. 2. The search of new compounds able to overcome MDR is of prime importance. 3. recently we have synthesized a new family of anthrapyridone compounds. The series contained derivatives modified with appropriate hydrophobic or hydrophylic substituents at the side chain. 4. The interaction of these derivatives with erythroleukina K562 sensitive and K562/DOX resistant (overexpressing P-glycoprotein) cell lines has been examined. The study was performed using a spectrofluorometric method which allows to continuously follow the uptake and efflux of fluorescent molecules by living cells. 5. It was demonstrated that the increase in the lipophilicity of anthrapyridones favoured the very fast cellular uptake exceeding the rate of P-gp dependent efflux out of the cell. For these derivatives, very high accumulation (the same for sensitive and resistant cells) was observed and the in vitro biological data confirmed that these compounds exhibited comparable cytotoxic activity towards sensitive and P-gp resistant cell line. In contrast, anthrapyridones modified with hydrophylic substituents exhibited relatively low kinetics of cellular uptake...


    70/355

    Tytuł oryginału: Carvedilol does not modulate moderate exercise-induced hyperkalemia in hemodialysis patients.
    Autorzy: Nowicki M., Szewczyk-Seifert G., Klimek D., Kokot F[ranciszek]
    Źródło: Clin. Nephrol. 2002: 57 (5) s.352-358, il., tab., bibliogr. s. 357-358
    Sygnatura GBL: 312,466

    Hasła klasyfikacyjne GBL:
  • farmacja
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background and aim: Non-selective á-adrenergic blockers may cause yperkalemia in patients with end-stage renal failure. In contrast, ŕ-adrenergic blockade has been found to decrease the hyperkalemic effect of physical exercise in healthy subjects, although we were unable to confirm this effect in hemodialysis patients. In a crossover design, we studied the effect of carvedilol, a non-selective á-adrenergic blocker with an additional ŕ1-blocking activity, on exercise-induced hyperkalemia in 17 anuric hemodialysis patients. Methods: All subjects were hiven either carvedilol (25 mg/day) or placebo for 2 weeks in a random order with a 2-week wash-out period. At the end of each treatment period they underwent a 30-minute exercise test on a bicycle ergometer with a fixed load of 20 W. Results: The treatment with carvedilol caused a significant decrease in blood pressure. Serum potassium before exercise tests was similar (5.37 ń 0.2 and 5.24 ń 0.2 mmol/l on carvedilol and placebo, respectively; mean ń SE). During the exercise, serum postassium oncreased significantly (p 0.001 in both tests) and subsequently decreased during 30 minutes of recovery (p 0.05). The mean rate of potassium increment during the exercise was similar (23.3 ń 3.3 ćmol/min on carvedilol and 20.0 ń 3.6 ćmol/l/min on placebo). during recovery, the mean rate of potassium decrement was 5.0 ń 3.0 ćmol/l/min and 6.7 ń 2.7 ćmol/l/min, respectively. Serum sodium, ionized calcium, insulin and plasma renin activity were similar before the exercise tests and did not change during the exercise. Conclusion: Carvedilol does not enhance the hyperkalemic effect of moderate physical exercise in anuric hemodialysis patients.


    71/355

    Tytuł oryginału: Expression of intercellular adhesion molecule-1 on the surface of peripheral blood and decidual lymhocytes of women with pregnancy-induced hypertension.
    Autorzy: Wilczyński Jacek R., Banasik Małgorzata, Tchórzewski Henryk, Głowacka Ewa, Malinowski Andrzej, Szpakowski Marian, Lewkowicz Przemysław, Wieczorek Artur, Zeman Krzysztof, Wilczyński Jan
    Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 102 (1) s.15-20, il., tab., bibliogr. 18 poz.
    Sygnatura GBL: 305,582

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska
  • ciąża
  • in vitro

    Streszczenie angielskie: Objective: If overexpression of intercelular adhesion molecule-1 (CD54) on lymphocytes exists it could have important implications for the pathophysiology of pregnancy-induced hypertension (PIH). Study design: CD54 "in vitro" expression (described as (p.c.) of CD54 + cells and CD54 mean fluorescence intensity, MFI) on the peripheral blood and decidual lymphocytes of pregnant with preeclampsia (PE) (n = 16), transient hypertension (TH) (n = 12), and controls (n = 9). Results: The percent (p.c.) of CD54 + peripheral blood lymphocytes, CD54 MFI on them and CD54 MFI on decidual lymphocytes were increased especially in PE. During PE the (p.c.) of CD54 decidual lymphocytes correlated negatively with platelet count. In TH the positive correlation between the blood pressure and the (p.c.) of CD54 peripheral blood lymphocytes as well as CD54 MFI on decidual lymphocytes were found. Conclusions: (1) PIH, especially PE, is accompanied by overexpression of intercellular adhesion molecule-1 (ICAM-1) on peripheral blood and decidual lymphocytes studied "in vitro", (2) Some of the parameters studied seem to correlate with clinical markers of PIH intensity but this fact needs further investigations using larger subject groups.


    72/355

    Tytuł oryginału: A multicentre randomized/controlled trial of a conventional versus modestly accelerated radiotherapy in the laryngeal cancer: influence of a 1 week shortening overall time.
    Autorzy: Hliniak A., Gwiazdowska B., Szutkowski Z., Kraszewska E., Kukolowicz P., Jarząbski A., Sochacka B., Mazurkiewicz M., Paprota K., Oliskiewicz W., Zadrożna O., Milecki P., Kubiak M., Czopkiewicz L., Jagas M., Góźdź S., Wieczorek A., Woytowicz A., Cisowska B., Magdziarz H., Nowakowski S., Kośniewski W., Laskosz I., Serafin A., Gradoń E.
    Źródło: Radiother. Oncol. 2002: 62 (1) s.1-10, il., tab., bibliogr. 13 poz.
    Sygnatura GBL: 312,846

    Hasła klasyfikacyjne GBL:
  • onkologia
  • otorynolaryngologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Background and purpose: To compare in a phase III study the loco-regional control, disease-free survival and overall survival induced by an accelerated regimen (AF) as compared with conventional regimen (CF) and to analyze the early and late post-radiation morbidity in both arms. Materials and methods: Patietns with ó 75, WHO 0-1, suitable for a radical course of radiotherapy T1-T3, NO, MO, stage of glottic and supraglottic laryngeal cancer were randomized to either CF: 66Gy given in 33 fractions over 45 days or AF: 66Gy given in 33 fractions over 38 days (2 fractions every Thrusday). A total of 395 patients were included from 05.1995 to 12.1998. Results. Early toxicity: At the end of radiotherapy patietns treated with AF complained for more severe reactions than patients treated with CF. In 8 weeks after treatment completion patients treated with AF complained only for more severe pain on swallowing (P = 0.027). In 4 months after treatment completion all types of toxicity except for skin teleangiectasia (P = 0.001) were similar in the two groups. Locoregional control: comparison between CF and AF showed no difference in terms of loco-regional control (P = 0.37). Conclusions: The improvement in AF in terms of loco-regional control is estimated to be 3-5 p.c. in comparison with conventional regimen and is not significant. The intensity of reactions after 4 months was similar in both arms, what suggests the possibility of further shortening of the overall time by few days or enhancing the total dose within the limits of acceptable morbidity.


    73/355

    Tytuł oryginału: The pathomechanism of posttraumatic edema of lower limbs. [P.] 1: The effect of extravasated blood, bone marrow cells, and bacterial colonization on tissues, lymphatics and lymph nodes.
    Autorzy: Szczęsny Grzegorz, Olszewski Waldemar L.
    Źródło: J. Trauma 2002: 52 (2) s.315-322, il., bibliogr. 31 poz.
    Sygnatura GBL: 305,046

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • psy
  • in vitro

    Streszczenie angielskie: Bacground: The mechanical injury of soft tissues and bones of lower extremities is frequently followed by long-lasting edema at the site of trauma and distally. The pathomechanisms of this complication remains unclear. Venous thrombosis and interruption of lymphatics are considered to be the main etiologic factors. We propose a concept that protracted healing of injured tissues and bones with involvement of the regional lymphatic system (lymphatics and nodes) is responsible for persistence of edema. The events affecting the first (scavenging) phase of healing of traumatized tissues, such as hematoma, translocation of bone marrow cells to soft tissues, and colonization by microorganisms, and in particular their effects on lymphatics and lymph nodes, were studied. Methods: Mongrel dogs weighing 15-20 kg were. Fresh blood or bone marrow cell (BMC) suspension was injected subcutaneously or intralymphatically into the paw. Strains of saprophyte bacteria residing on the skin surface were cultured and injected intradermally. Oilcontrast lymphography was performed before and after injections to evaluate the changes in lymphatics and nodes. Biopsy samples of paw skin, subcutaneous tissue, and regional lymph nodes (LN) were taken. The responsiveness of LN lymphocytes was studied in autologous mixed cultures with peripheral blood lymphocytes (PBL), BMC, and cultured bacteria. Results: The PBL from subcutaneously injected blood were evacuated by the lymphatic route at a rate of 1-3 proc./6 hr. There was no thrombosis of lymphatic vessels or obstruction of LN sinusoids...


    74/355

    Tytuł oryginału: Long-term changes in calbindin D28K immunoreactivity in the rat hippocampus after cardiac arrest.
    Autorzy: Sadowski Marcin, Lazarewicz Jerzy W., Jakubowska-Sadowska Katarzyna, Wiśniewski Henryk M., Mossakowski Mirosław J., Brown W. Ted
    Źródło: Neurosci. Lett. 2002: 321 (1/2) s.90-94, il., bibliogr. 20 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Calbindin D28K (CB) expression was analyzed in the rat hippocampus following 10-min-cardiac arrest-induced ischemia within a year after reperfusion. In rats examined 3 days after ischemia, CB immunoreactivity disappeared completely from CA1 pyramidal neurons and from most CA2 pyramids. In the stratum granulosum of the dentate gyrus, mossy fibers, and hippocampal interneurons, CB immunoreactively was preserved, although staining was somewhat paler than that in control rats. A similar pattern of CB immunoreactively was found in rats sacrificed 14 days and 1 month after cardiac arrest. From the 14th postischemic day, neuronal loss in the stratum pyramidale of CA1 but not in that of CA2 became apparent. The reappearance of CB immunoreactivity in CA1 and CA2 pyramidal neurons was noticed 6 months after ischemia, and the pattern was identical to that observed in animals sacrificed 12 months after the octus. The prolonged loss and delayed reappearance of CB immunoreactivity in the hippocampus demonstrate that ischemia may induce long-term disturbances of protein expression, which may in turn result in impairment of hippocampal functioning.


    75/355

    Tytuł oryginału: Induction of oxidative DNA damage by carcinogenic metals.
    Autorzy: Bal Wojciech, Kasprzak Kazimierz S.
    Źródło: Toxicol. Lett. 2002: 127 (1/3) s.55-62, bibliogr. s. 60-62
    Sygnatura GBL: 306,158

    Hasła klasyfikacyjne GBL:
  • farmacja
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: The metal ions carcinogenic to humans are As, Be, Cd, Cr and Ni, and the candidates also include Co, Cu, Fe and Pt. A range of molecular mechanisms was proposed for these metals, reflecting their diverse chemical properties. The oxidative concept in metal carcinogenesis proposes that some complexes of the above metals (Co, Cr, Cu, Fe, Ni) formed in vivo undergo redox cycling, yielding reactive oxygen species and/or high valence metal ions which oxidize DNA. Some of the products of oxidative DNA damage, including 8-oxoguanine and strand breaks, induce mutations, which may lead to neoplastic transformation. The establishment of metal-binding modes in the cell nucleus and of their reactivity is crucial for the understanding of molecular events in metal carcinogenesis. We have proposed the binding sites for Ni(II) and Cu(II) in core histones (H3, H2A) and sperm protamines (HP2) and, using molecular models, provided evidence for the generation of promutagenic oxidative DNA damage by the bound metals.


    76/355

    Tytuł oryginału: Novye struktury 0-cpecificheskikh polisakharidov bakterijj roda Proteus. 2: Polisakharidy, soderzhashhie O-acetilnye gruppy.
    Tytuł angielski: New structures of the O-specific polysaccharides of Proteus. [P.] 2: Polysaccharides containing O-acetyl groups.
    Autorzy: Kondakova A. N., Toukach F. V., Senchenkova S. N., Arbatsky N. P., Shashkov A. S., Knirel Y. Ju., Zych K., Torzewska A., Rożalski A., Kołodziejska K., Sidorczyk Z.
    Źródło: Biokhimija 2002: 67 (2) s.240-252, il., tab., bibliogr. 36 poz., sum.

    Hasła klasyfikacyjne GBL:
  • immunologia
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Structures of five new O-specific polysaccharides of the lipopolysaccharides of bacteria Proteus were established. Four of them, Proteus penneri 4 (072), Proteus vulgaris 63/57 (037), Proteus mirabilis TG 277 (069), and Proteus penneri 20 (017), contain O-acetyl groups in non-stoichiometric quantities, and the polysaccharide of P. penneri 1 st structuraly related to that of P. penneri 4. The structures were elucidated using NMR spectroscopy, including one-dimensional 1H nd 13C NMR, two-dimensional 1H, 1H correlation (COSY, TOCSY), 1H, 13C heteronuclear multiple-quantum coherence (HMQC) and multiple-bond correlation spectroscopy, as well as nuclear Overhauser effect spectroscopy (NOESY or ROESY), along with chemical methods. The structural data obtained are useful as the chemical basis for the creation of the classification scheme for Proteus strains.


    77/355

    Tytuł oryginału: Novye struktury O-specificheskikh polisakharidov bakterijj roda Proteus. 1: Fosfatsoderzhashhie polisakharidy.
    Tytuł angielski: New structures of the O-specific polysacchardies of Proteus. [P.] 1: Phosphate-containing polysaccharides.
    Autorzy: Toukach F. V., Kondakova A. N., Arbatsky N. P., Senchenkova S. N., Shashkov A. S., Knirel Y. A., Zych K., Rożalski A., Sidorczyk Z.
    Źródło: Biokhimija 2002: 67 (2) s.318-331, il., tab., bibliogr. 23 poz., sum.

    Hasła klasyfikacyjne GBL:
  • immunologia
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: The O-specific polysaccharide chains (O-antigens) of the lipopolysacchardides of five Proteus strains, P. vulgaris 017, P. mirabilis 016 and 033, and P. penneri 31 and 103, were found to contain phosphate groups that link the non-sugar components, namely, ethanolamine and ribitol. From them, the polysaccharides of P. mirabilis 016 and P. penneri 103 include ribitol phosphate in the main chain and thus resemble ribitol teichoic acids of Gram-positive bacteria. The structures of the polysaccharides were elucidated using NMR spectroscopy, including two-dimensional 1H, 1H correlation spectroscopy (COSY and TOCSY), nuclear Overhauser effect spectroscopy (NOESY or ROESY), and H-detected 1H, 13C, and 1H, 31P heteronuclear multiple-quantum coherence (HMQC), along with chemical methods. The structures determined are unique among the bacterial polysaccharides and, together with data obtained earlier, represent the chemical basic for classification of Proteus strains. Based on structural similarities of the O-specific polysaccharides and serological relationships between the O-antigens, we propose to add P. penneri strain 31 to serogroup 019 and to extend serogroup 017 by including P. penneri strain 16.


    78/355

    Tytuł oryginału: Effects of acamprosate and some polyamine site ligands of NMDA receptor on short-term memory in rats.
    Autorzy: Mikołajczak Przemysław, Okulicz-Kozaryn Irena, Kamińska Ewa, Niedopad Lidia, Polańska Anna, Gębka Joanna
    Źródło: Eur. J. Pharmacol. 2002: 444 (1/2) s.83-96, il., tab., bibliogr. s. 94-96
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The aim of this study was to evaluate the effect of multiple acamprosate (500.0 mg/kg, p.o.) administration on short-term memory, using te social recognition test in rats. Ifenprodil (1.0 mg/kg, i.p.), arcaine (5.0 mg/kg, i.p.) and spermidine (20.0 mg/kg, i.p.) were chosen as polyamine ligands and their action or interaction with acamprosate was also studied. The doses used did not show any sedative activity, which was assessed by measuring locomotor activity and the hypnotic effect of ethanol. The findings suggest that acute acamprosate treatment did not impair short-term memory. Multiple acamprosate and a single spermidine or arcaine administration led to better performance in the memory test, whereas no significant difference was observed in ifenprodil-treated rats. Co-administratio of a single arcaine or spermidine dose with multiple acamprosate produced worse results. This means that the effect of repeated acamprosate administration can be changed by the co-administration of other polyamine ligands, so that care should be taken in interpreting.


    79/355

    Tytuł oryginału: Repeated imipramine and electroconvulsive shock increase ŕ1A-adrenoceptor mRNA level in rat prefrontal cortex.
    Autorzy: Nalepa Irena, Kreiner Grzegorz, Kowalska Marta, Sanak Marek, Zelek-Molik Agnieszka, Vetulani Jerzy
    Źródło: Eur. J. Pharmacol. 2002: 444 (3) s.151-159, il., tab., bibliogr. s. 158-159
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: ŕ1-Adrenoceptors have been implicated in the mechanism of action of antidepressants, but their action on specific receptor subtypes was rarely reported. We compared now the action of two prototypic antidepressant treatments: repeated imipramine and electroconvulsive shock, on the expression of the ŕ1A - and ŕ1B -adrenoceptor mRNAs and on the receptor density in rats. The mRNA expression was assessed by Northern blot in the prefrontal cortex and the hippocampus, the receptor density was measured by [3H] prazosin binding in the total cerbral cortex and hippocampus. In the cortex, both treatments elevated the ŕ1A -adrenoceptor mRNA and the expression of receptor protein. The expression of ŕ1B -adrenoceptor mRNA remained unaffected. In contrast, in the hippocampus, the antidepressant treatments augmented the density of ŕ1A -adrenoceptor protein without changing the level of its mRNA expression there. The results suggest that the ŕ1A -adrenoceptor subtype is specifically involved in the mechanism of action of classical antidepressant treatments.


    80/355

    Tytuł oryginału: Antigen levels of the urokinase-type plasminogen activator and its gene polymorphisms in colorectal cancer.
    Autorzy: Przybyłowska K., Smolarczyk K., Kulig A., Romanowicz-Makowska H., Dziki A., Ulańska J., Pander B., Błasiak J.
    Źródło: Cancer Lett. 2002: 181 (1) s.23-30, il., tab., bibliogr. 18 poz.
    Sygnatura GBL: 306,023

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • onkologia
  • genetyka
  • immunologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We analysed the distribution of genotypes of two polymorphisms in the urokinas-type plasminogen activator (uPA) gene: C - T substitution in exon 6 and T - C substitution in intron 7 in 52 subjects with colorectal cancer. Genotypes were determined in tumour tissue and distant mucosa samples by allele-specific polymerase chain reaction. The antigen levels of uPA in cancer tissue were higher than in distant mucosa as measrued by enzyme-linked immunosorbent assay. The level of uPA antigens in cancer samples with the C/C genotype of C - T polymorphism in exon 6 was higher than in samples with C/T and T/T genotypes. No differences in the level of uPA antigens between the alleles of the intron 7 T - C polymorphism were found. As uPA can be involved in cancer invasion and metastasis, C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression.


    81/355

    Tytuł oryginału: Hemodynamic performance after stented vs stentless aortic valve replacement.
    Autorzy: Jasiński M. J., Hayton J., Kadziola Z., Woś S[tanisław], Sosnowski A. W.
    Źródło: J. Cardiovasc. Surg. 2002: 43 (3) s.313-317, il., tab., bibliogr. 10 poz.
    Sygnatura GBL: 311,845

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • kardiologia
  • transplantologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kliniczna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background. Stentless bioprostheses are anticipated to cause improved hemodyanmics and increased longevity over stented bioprosthesis. We have compared echocardiographic analysis of stented bioprosthesis "Freestyle" with stented "Mosaic" bioprosthesis. Because of similar technology (0 pressure fixation, anticalcification) any differences may relate to stent. Methods. Twenty-eight patients undergoing AVR were randomly assigned to receive either stented or stentless. Echocardiograms, by means of M-mode and Doppler were performed early, 3-6 months and 1 year postoperatively. Results. The peak flow velocity was significantly lower in teh stentless group, especially 1 week and 6 months after surgery. Mean transvalvular gradient dropped significantly in stentless group and did not change in stented group. EOA did not change significantly in either of groups. AoV velocity time integral was increasing in stentless group. LV mass had fallen significantly in both groups but degree of mass reduction was comparable. Conclusions. There are marked improvements of stentless valves hemodynamics. However it is not necessary equal to higher degree of LV mass reduction during 1 year follow-up.


    82/355

    Tytuł oryginału: Substance P content in the cerebrospinal fluid and fluid perfusing cerebral ventricles during elicitation and inhibition of trigemino-hypoglossal reflex in rats.
    Autorzy: Zubrzycka Maria, Janecka Anna
    Źródło: Brain Res. 2002: 941 (1/2) s.29-33, il., bibliogr. 47 poz.
    Sygnatura GBL: 312,085

    Hasła klasyfikacyjne GBL:
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The aim of this study was to establish whether tooth pulp and periaqueductal central gray (PAG) stimulation affects the release of substance P (SP) into the fluid perfusing the cerebral ventricles in rats. The content of substance P in the cerebrospinal fluid and fluid perfusing cerebral ventricles was determined during incisor pulp stimulation with electrical impulses inducing nociceptive trigeminohypoglossal reflex and then during inhibition of the reflex by stimulation of PAG. Perfusion of the cerebral ventricles was carried out using artificial cerebrospinal fluid (aCSF). SP-like immunoreactivity (SP-LI) was determined in the samples by radioimmunoassay. Samples were collected in four groups: first group - cerebrospinal fluid (CSF); second group - aCSF perfusates without stimulation; third group-aCSF perfusates during incisor pulp stimulation; fourth group-aCSF perfusates during incisor pulp stimulation and simultaneous inhibition of trigemino-hpoglossal reflex by PAG stimulation. It was shown that incisor pulp stimulation led to the increased release of SP-LI into the fluid perfusing cerebral ventricles. Stimulation of PAG reduced the release of SP-LI into the cerebro-ventricular system to the values obtained before the tooth pulp stimulation. The results indicate that PAG significantly inhibits the release of SP-LI into the rat cerebral ventricular system and may be involved in the inhibition of trigemino-hypoglossal reflex.


    83/355

    Tytuł oryginału: The beginnings of urology in Kraków: on the 70th anniversary of the founding of the Clinic of Urology.
    Autorzy: Dobrowolski Z. F., Swolkien J., Dobrowolska B., Lipczyński W., Jaszczyński J.
    Źródło: Br. J. Urol. 2002: 89 (6) s.596-598, bibliogr. 4 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • urologia

    Typ dokumentu:
  • praca historyczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • historia nowożytna
  • historia XIX wieku
  • historia XX wieku


    84/355

    Tytuł oryginału: Survival in patients with rare subtypes of renal cell carcinoma.
    Autorzy: Grabowski M[arek], Huzarski T., Lubiński J., Sikorski A[ndrzej]
    Źródło: Br. J. Urol. 2002: 89 (6) s.599-600, tab., bibliogr. 14 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Objective. To evaluate the survival of patietns with rare malignant histological subtypes of renal cancer. Pateitns and methods. The Heidelberg classification of renal cell carcinoma (RCC) divides tumours into clear cell carcinoma (CCC), papillary cancer (PC), chromophobic cancer (ChC) and collecting duct carcinoma (CDC). Sarcomatoid tumours are in a different subgroup treated as a final stage of histological progression. Between 1990 and 1997, 319 nephrectomies were undertaken because of RCC in 317 patients. In 42 patietns (13 p.c.) the patholgoical findings showed other than CCC; in 13 PC was confirmed histolgoically, in nine ChC, in 11 a mixed type of CCC and sarcomatoid type, in seven a sarcomatoid tumour and in four, CDC. Results. One patietn of the 13 with PC and two of the nine with ChC died. The worst prognosis was in those with CDC, CCC-sarcomatoid and sarcomatoid tumours, as all these patietns died. Conclusion. The histopathological differentiation of RCC into subtypes gives additional useful prognostic information.


    85/355

    Tytuł oryginału: Assessing the vascular-stromal coefficient in patients with benign prostatic hyperplasia or prostate cancer using transrectal ultrasonography and power Doppler analysis.
    Autorzy: Dobrowolski Z., Jaszczyński J., Drewniak T., Habrat W.
    Źródło: Br. J. Urol. 2002: 89 (6) s.601-603, il., tab., bibliogr. 10 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • radiologia
  • urologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska

    Streszczenie angielskie: Objectives. To assess a coefficient of theprotatic blood supply in patietns with prostate cancer or benign prostatic hyperplasia (BPH), and thus improve the diagnostic and prognostic variables needed to evaluate patients with prostate cancer. Patietns and methods. The vascular-stromal coefficient was defined as the ratio of the blood vessel surface area and the surface area of the prostate, using power Doppler functions and transrectal ultrasonography. The index was calcualted for the plane of the prostatic surface from the apex to the widest points of the peripheral zone, and for the plane across the highest density of blood vessels in the transition zone. A micobubble contrast agent was used to intensify the acoustic signals from prostatic vessels. The images were reconstructed in three dimensions. In all, 61 patietns with BPH and 32 with confirmed prostate cancer were examined, and compared with a control group of young men. Results. In the horizontal plane crossing the apex of the prostate, the vascular-stromal coefficient was 0.0054 for the controls, 0.0072 for BPH and 0.0067 for cancer. At the level of the highest density of blood vessels the respective values were 0.0083, 0.038 and 0.029. The respective values for the first plane after using the contrast agent were 0.025, 0.092 and 0.0365, and in the second level 0.04, 0.15 and 0.14. Conclusion. The vascular-stromal coefficient measured at both levels was higher in patietns with BPH than in those with cancer or than in the controls. The prostatic vascular system can be computer-reconstructed in three dimensions, allowing an evaluation of asymmetry and other vascular abnormalities...


    86/355

    Tytuł oryginału: Correlations among prostatic-specific antigen, Gleason score, staging and grading in patients after radical prostatectomy.
    Autorzy: Szymanowski J., Dudek P., Ratajski J., Wysocki M.
    Źródło: Br. J. Urol. 2002: 89 (6) s.612-613, tab., bibliogr. 6 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia
  • chirurgia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska

    Streszczenie angielskie: Objectives. To evaluate the correlation between Gleason grade, T stage, tumour grade and preoperative prostatespecific antigen (PSA) level ( or 20 ng/mL) in patietns after radical prostatectomy (RP). Patietns and methods. The serum total PSA level was measured 3 months before surgery in 87 patients who then undrewent RP between 1994 and 2000; the tumour specimens were staged and graded. Results. There were 57 (66 p.c.) patietns with a PSA level of 20 ng/mL; in this group only four (7 p.c.) patients had a Gleason grade of 7 and eight (14 p.c.) had G3 tumours. Thirty (34 p.c.) patients had a PSA level of 20 ng/mL; in this group only two (7 p.c.) patietns had a Gleason grade of 7 and 26 (87 p.c.) had G1 and G2 tumours. Conclusions. These results confirm our previous observations that the PSA level cannot be use as the only factor to indicate RP in patients with prostate cancer, but further evaluation on more patietns is needed.


    87/355

    Tytuł oryginału: Vascular angiographic asymmetry on three-dimensional transrectal power Doppler ultrasonography in patients with organ-confined prostate cancer.
    Autorzy: Dobrowolski Z. F., Jaszczyński J., Drewniak T., Habrat W., Kusionowicz J.
    Źródło: Br. J. Urol. 2002: 89 (6) s.614-615, bibliogr. 7 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • radiologia
  • urologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska

    Streszczenie angielskie: Objective. To assess whether organ-confined prostate cancer not detected on routine transrectal ultrasonography (TRUS) can be detected using threedimensional (3-D) power Doppler methods. Patients and methods. A preliminary trial of the use of TRUS with 3-D power Doppler image reconstruction was conducted in 116 patients being evaluated for lower urinary tract symptoms. Using a microbubble enhancing agent, a adequate horizontal plane allowed the simultaneous visualization and assessment of the symmetry of echogenicity and vascularization of the peripheral area of both prostate lobes, which is not possible in standard TRUS. The peripheral zone vascular asymmetry in the horizontal plane was also assessed retrospectively n those patients with pT2a,b prostate carcinoma. Results. Stage pT2a,b prostate carcinoma was confirmed in 26 patients; in this group, two independent investigators confirmed the vascular asymmetry between the left and right peripheral zone, in the horizontal plane, in 22 patients, giving a sensitivity of 85 p.c. Conclusions. Vascular asymmetry allows guided biopsy in lesions that are invisible using standard TRUS. Microbubble agents are an effective method of increasing the acoustic signals from small vessels of the prostate.


    88/355

    Tytuł oryginału: Evaluation of the influence of systemic neoadjuvant chemotherapy on the survival of patietns treated for invasive bladder cancer.
    Autorzy: Kolaczyk W., Dembowski J[anusz], Lorenz J[erzy], Dudek K.
    Źródło: Br. J. Urol. 2002: 89 (6) s.616-619, il., bibliogr. 13 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia
  • farmacja

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Objective. To assess the influence of neoadjuvant systemic chemotherapy using a modified methotrexate, vinblastine, doxorubicin and cisplatin (MVAC) scheme in patietns with invasive bladder cancer. Patients and methods. Two groups of patients were reviewed retrospectively: group 1 included 51 who received chemotherapy before cystectomy and group 2 included 62 who were treated only with surgery. The mean (range) duration of follow-up was 3.2 (0.25 - 10.25) years. The patients in group 1 were divided into two subgroups: those with tumour confined to the bladder (T1, T2 and T3a) and the remaining patients with tumour beyond the bladder (T3b, T4a,b). The chemotherapy was administered as routine MVAC, except vinblastine and methotrexate were given at 15 and 22 days during the cycle. A mean of three cycles were administered. Results. The 5-year survival rate in group 1 and 2 was 66 p.c. and 58 p.c., respectively (P 0.3); after 8 years of follow-up the survival rates were 58 p.c. and 33 p.c., respectively, and significantly different (P 0.01). Conclusion. Systemic chemotherapy using the modified MVAC scheme in patients subsequently undergoing radical cystectomy improved the survival rate after 8 years of follow-up.


    89/355

    Tytuł oryginału: Treatment of high-risk superficial bladder cancer with maintenance bacille Calmette-Gu‚rin therapy: preliminary results.
    Autorzy: Kołodziej A., Dembowski J[anusz], Zdrojowy R[omuald], Woźniak P., Lorenz J[erzy]
    Źródło: Br. J. Urol. 2002: 89 (6) s.620-622, tab., bibliogr. 6 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia
  • toksykologia
  • immunologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective. To evaluate, in a prospective study, the effects and results of maintenance therapy with bacille Calmette-Gu‚rin (BCG) in treating patietns with high-risk superficial bladder cancer. Patients and methods. In all, 155 patietns were enrolled in a randomized study of transurethral resection alone (53) or combined with intravesical BCG (102) as a treatmetn for superficial bladder cancer. BCG was administered for six consecutive weeks followed by three weekly instillations in months 3, 6, 12, 18, 24, 30 and 36 after resection. Recurrence, progression, prognostic factors and side-effects were assessed and analysed. Results. After a median (range) followup of 23 (6-42) months, 83 of the 102 patietns treated with BCG (81 p.c.) were disease-free, compared with 24 of the 53 treated with resection alone (45 p.c.). There was also a significant difference in tumour progression and time to progression between the trial arms. The disease progressed in eight patients (8 p.c.) treated with BCG and in 12 (23 p.c.) of those treated by resection alone. Independent risk factors for progression were DNA ploidy status and stage. Only the completion of treatment was predictive of outcome (risk of recurrence) for patietns treated with BCG. Conclusion. Maintenance BCG therapy was better than resection alone in reducing the incidence of recurrence and progression in patietns with high-risk superficial bladder cancer.


    90/355

    Tytuł oryginału: Superficial bladder cancer T1G3: the choice of treatment.
    Autorzy: Borkowski A[ndrzej]
    Źródło: Br. J. Urol. 2002: 89 (6) s.623-627, tab., bibliogr. 41 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie


    91/355

    Tytuł oryginału: T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia - the three-color flow cytometry analysis.
    Autorzy: Podhorecka Monika, Dmoszyńska Anna, Roliński Jacek, Wąsik Ewa
    Źródło: Leuk. Res. 2002: 26 (7) s.657-660, il., bibliogr. 8 poz.
    Sygnatura GBL: 306,151

    Hasła klasyfikacyjne GBL:
  • immunologia
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The impairments in immune cell functions as well as changes in cytokine network are the cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic limphocytic leukemia (B-CLL). To broaden the knowledge of immune system in B-CLL we tried to evaluate the Th1/Th2 and Tc2 balance in B-CLL patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNç expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells. The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression.


    92/355

    Tytuł oryginału: Morphine attenuates pain and prevents inflammation in experimental peritonitis.
    Autorzy: Płytycz Barbara, Natorska Joanna
    Źródło: Trends Immunol. 2002: 23 (7) s.345-346, il., bibliogr. 13 poz.
    Sygnatura GBL: 312,774

    Hasła klasyfikacyjne GBL:
  • farmacja
  • gastroenterologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy


    93/355

    Tytuł oryginału: Primenenie mediastinoskopii w diagnostike khirurgicheskikh zabolevanij organov grudnoj polosti.
    Tytuł angielski: Application of mediastinoscopy in diagnosis of surgical diseases of the thoracic cavity organs.
    Autorzy: Pieluchowski S. W.
    Źródło: Klin. Khir. 2002 (1) s.58-59, bibliogr. 5 poz.
    Sygnatura GBL: 303,753

    Hasła klasyfikacyjne GBL:
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • komunikat
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska


    94/355

    Tytuł oryginału: Oslozhnenija posle operacij s primeneniem videotorakoskopii i ikh lechenie.
    Tytuł angielski: Complications after operation with videothoracoscopy application and their treatment.
    Autorzy: Pieluchowski S. W., Piwkowskij C., Kasprzak P.
    Źródło: Klin. Khir. 2002 (2) s.30-31, bibliogr. 6 poz., sum.
    Sygnatura GBL: 303,753

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Of 4715 patient, who were present in 1995-2000 yrs in the clinic, in 708 (15 p.c.) operation, using videothoracoscopy method, was performed. In 539 (76.1 p.c.) of patietns the method was applied for diagnosis and in 169 (23.9 p.c.) - for treatment. After the operation 365 patients were observed, complications have occurred in 14, including the early - in 10 (2.7 p.c.) and the late - in 4 (1.1 p.c.). All the patients have survived.


    95/355

    Tytuł oryginału: Propranolol and metoprolol enhance the anticonvulsant action of valproate and diazepam against maximal electroshock.
    Autorzy: Luchowska Elżbieta, Luchowski Piotr, Wielosz Marian, Kleinrok Zdzisław, Czuczwar Stanisław J., Urbańska Ewa M.
    Źródło: Pharmacol. Biochem. Behav. 2002: 71 (1/2) s.223-231, il., tab., bibliogr. [40] poz.
    Sygnatura GBL: 312,498

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: The anticoconvulsive potential of classical antiepileptics co-administered with á-adrenergic receptor antagonists against generalized tonic-clonic seizures was evaluated in the model of maximal electroshock (MES)-induced convulsions. Propranolol, acebutolol, metoprolol and atenolol were tested in the doses not affecting the electroconvulsive threshold. Propranolol and metoprolol lowered the ED50 of valproate and diazepam. Acebutolol reduced valproate's but not diazepam's ED50 value. In contrast, hydrophilic atenolol, not penetrating via blood-brain barrier, affected neither the action of valproate nor diazepam. None of the studied drugs changed the protective activity of carbamazepine and phenytoin against MES. á-blokers per se did not alter the motor performance of mice. Moreover, propranolol and metoprolol did not influence diazepam-evoked impairment of locomotor activity. The free plasma and brain levels of antiepileptic drugs were not affected by á-blokers. In conclusion, the use of certain á-adrenoceptor antagonists, such as propranolol and metroprolol, might improve the antiepileptic potential of valproate and diazepam.


    96/355

    Tytuł oryginału: Implication of immunological abnormalities after adenotonsillotomy.
    Autorzy: Zielnik-Jurkiewicz Beata, Jurkiewicz Dariusz
    Źródło: Int. J. Pediatr. Otorhinolaryngol. 2002: 64 (2) s.127-132, tab., bibliogr. 20 poz.
    Sygnatura GBL: 306,189

    Hasła klasyfikacyjne GBL:
  • pediatria
  • immunologia
  • chirurgia
  • otorynolaryngologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca kliniczna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: The adenoids and tonsils are thought to be essential parts of the system protecting organism against pathogens invading the upper respiratory tracts. Human adenoids and tonsils are known to be immunologically reactive lymphoid organs, which manifest specific antibodies and B and T cells activity in response to variety of antigens carrying out the functions of humoral and cellular immunity. The purpose of the study was to observe the changes in systemic immunity in children with hypertrophy of adenoids and tonsils treated in Department of Laryngology, Children's Hospital in Warsaw in period 1994-1999. The study comprised 80 patients (33 girls and 47 boys, aged from 3 to 14 years, mean age 6.8 years) with diagnosed hypertropphy of adenoids and tonsils. The diagnosis of hypertrophy of adenoids and tonsils was based on characteristic history and laryngological examination. All patients were scheduled for adenotonsillotomy. The control group comprised 40 people (14 girls and 26 boys, aged from 3 to 15 years, mean age 7.6 years) without hstory of the recurrent upper tract infections. In all patients we carried out following examinations: serum levels of immunoglobulins A, G, M (humoral immunity); percentage of T lymphocytes (CD3); percentage of T helper (CD4) and T cytotoxic (CD8) lymphocytes (cellular immunity) and delayed cutaneous hypersensitivity - Multitest CMI (cell mediated immunity). Our study demonstrate that in children with hypertrophy of adenoids and tonsils exist changes in the immunological parameters...


    97/355

    Tytuł oryginału: Ultrasound and biochemical evaluation of liver regeneration process after resections for primary and metastatic tumors.
    Autorzy: Kulig Jan, Nowak Wojciech, Popiela Tadeusz, Sobocki Jacek, Zarębski Bartosz
    Źródło: Hepatogastroenterology 2002: 49 (44) s.349-353, il., tab., bibliogr. 20 poz.
    Sygnatura GBL: 304,355

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: background/Aims: The liver regeneration process after resective procedures is an important clinical problem especially in view of the growing population of patients requiring liver transplantations or undergoing resective procedures as well as those with toxic or viral liver injures. The aim of the study was to analyze liver regeneration potential using ultrasound examination, and liver function biochemical tests in the patients after liver resections. Methodology: Twenty-one patients with primary or metastatic liver cancer were included in the study (17 males, 4 females, mean age: 59.8, SD 10:3). The patients were undergoing gastrointestinal tract primary tumor resections with extended lymphandenectomy and liver metastatic tumor excisions. Liver volume was measured postoperatively in the follow-up ultrasound examinations and biochemical tests were performed before and after the procedure. Liver function and activity of p-450 cytochrome was tested with IRIS 13C stable isotope breath analyzer. Results: The mean time of follow-up was 12.6 months (SD 7.8) during which volume of liver increased on average in the first five months to 105.7, 112.1, 125.7, 131.2, and 138.4 p.c. of the immediate postoperative value. Biochemical parameters returned to normal after 2-3 weeks. Four patients in whom liver failed to regenerate died due to cancer spread in the mean time of 6.5 months. Conclusions: Liver has tremendous regeneration potential. In spite of this fact, extended hepatectomies affect largely the functionaln status of residual parenchyma which may lead to a fatal outcome.


    98/355

    Tytuł oryginału: Chemokine growth-regulated-ŕ: a possible role in the pathogenesis of endometriosis.
    Autorzy: Szamatowicz J[acek], Laudański P., Tomaszewska I., Szamatowicz M[arian]
    Źródło: Gynecol. Endocrinol. 2002: 16 (2) s.137-141, il., tab., bibliogr. 15 poz.
    Sygnatura GBL: 313,072

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Endometriosis is a common disease in women at reproductive age. We investigated the concentration of neutrophil-activating factor (growth-regulated gene-ŕ, GRO-ŕ) (a member of the chemokine family), in peritoneal fluid of infertile women with or without endometriosis. Peritoneal fluid was obtained laparoscopically from 22 women with and 21 without visible endometriotic lesions. GRO-ŕ concentration was measured by the use of an ELISA kit. Median concentration of GRO-ŕ was 87.65 ń 56.19 pg/ml in the study group and 60.72 ń 11.98 pg/ml in the control group. The distribution of data differed from normal, therefore logarithmic transformation of data was performed. Concentration of GRO-ŕ was significantly higher in the peritoneal fluid of women with endometriosis when compared with controls (p = 0.05). No correlation between concentration of GRO-ŕ, stage of endometriosis, duration of infertility or sex steroid hormone levels was found. The study shows that GRO-ŕ may play a role in the pathogenesis of endometriosis, possibly by chemoattraction and activation of neutrophils present in higher numbers in the peritoneal fluid of women with endometriosis. It is also feasible that the angiogenic properties of GRO-ŕ might prompt the progression of endometriotic lesions.


    99/355

    Tytuł oryginału: Clinical, electrophysiological and immunological remissions after thymectomy in myasthenia gravis.
    Autorzy: Kostera-Pruszczyk A[nna], Emeryk-Szajewska B., Świtalska J., Strugalska-Cynowska H., Rowińska-Marcińska K[atarzyna], Nowak-Michalska T., Szyluk B.
    Źródło: Clin. Neurophysiol. 2002: 113 (4) s.615-619, il., tab., bibliogr. s. 618-619
    Sygnatura GBL: 311,330

    Hasła klasyfikacyjne GBL:
  • pediatria
  • chirurgia
  • neurologia
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Objectives: Approximately 50 p.c. of patients treated with thymectomy have a chance for symptom-free life. However, immunological and neurophysiological abnormalities may be detected in patients with clinical remission. Although improvement usually parallels decrease in acetylcholine receptor antibody (AChRAb) levels and jitter values, there is a question what factors influence immunological and electrophysiological remission in a population of myasthenia gravis (MG) patients. Methods: We analyzed retrospectively clinical data of 32 MG patients operated for generalized MG, followed-up at our department for 17.2 (4-31) years. They were in clinical remission for 12.8 (2-25) years. All of them had single fiber electromyography (SFEMG) of extensor digitorum communis muscle (EDC) muscle and estimation of AChRAb level at the end of follow-up. Their age at onset of MG was 17 years (6-48) and at thymectomy 19 (6.4-58) years. Tensilon test was positive in 30, repetitive nerve stimulation in 29 cases. Results: Clinical remission was reached on average 4.2 years after thymectomy. SFEMG jitter value normalized in 60 p.c. of cases, AChRAb were negative only in 34 p.c. of patients. Jitter values correlated with AChRAb levels (P = 0.006,, r = 0.5) but were not related to clinical factors. Only time to thymectomy correlated with time from thymectomy to clinical remission (P = 0.001, r = 0.5). Conclusions: Clinical remission is not always accompanied by normalization of SFEMG and AChRAb. Although normalization of neuromuscular transmission in patients with remission of MG is individual, short duration of MG before thymectomy increases the chance of early remission.


    100/355

    Tytuł oryginału: Asymmetry of frameshift mutagenesis during leading and lagging-strand replication in Escherichia coli.
    Autorzy: Gaweł Damian, Jończyk Piotr, Białoskórska Małgorzata, Schaaper Roel M., Fijałkowska Iwona J.
    Źródło: Mutat. Res 2002: 501 (1/2) s.129-136, il., tab., bibliogr. 33 poz.
    Sygnatura GBL: 304,952

    Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Mutations in DNA, including frameshifts, may arise during DNA replication as a result of mistakes made by the DNA polymerase in copying the DNA template strands. In our efforts to better understand the factors that contribute to the accuracy of DNA replication, we have investigated whether frameshift mutations on the Escherichia coli chromosome occur differentially within the leading and lagging-strands of replication. The experimental system involves measurement of the reversion frequency for several defined lac frameshift alleles in pairs of strains in which the lac target is oriented in the two possible directions relative to the origin of chromosomal replication. Within these pairs any defined lac sequence will be subject to leading-strand replication in one orientation and to lagging-strand replication in the other. Fidelity differences between the two modes of replication can be observed as a differential lac reversion between the two strains. Our results, obtained with a series of lac alleles in a mismatch-repair-defective background, indicate that for at least some of the alleles there is indeed a difference in the fidelity of replication between the two modes of replication.


    101/355

    Tytuł oryginału: Decreased apoptosis and distinct profile of infiltrating cells in the nasal polyps of patients with aspirin hypersensitivity.
    Autorzy: Kowalski M. L., Grzegorczyk J., Pawliczak R., Kornatowski T., Wagrowska-Danilewicz M., Danilewicz M.
    Źródło: Allergy 2002: 57 (6) s.493-500, il., tab., bibliogr. 49 poz.
    Sygnatura GBL: 302,302

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • immunologia
  • otorynolaryngologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Background: Patients with aspirin-hypersensitive rhinosinusitis/asthma suffer from a severe form of hyperplastic rhinosinusitis with recurrent polyposis. We aimed to assess the presence of apoptotic cells in nasal polyps from aspirin-hypersensitive (AH) and aspirin-tolerant (AT) patients with rginosinusitis as related to the characteristis of local inflammation. Methods: Nasal polyps obtained from 16 AH patients and 36 AT patients (17 atopic and 19 nonatopic) were stained for eosinophils and metachromatic cells, and in parallel immunocytochemistry was performed to detect CD45RO+, HLA-DR+, CD8+ and CD68+ positive cells. Apoptotic cells were detected by a nick-end labelling technique, TUNEL. Results: The density of apoptotic cells in AH polyps (5.5 + 1.5 cells/mmý) was significantly lower as compared to both atopic (18.7 + 3.8 cells/mmý; P 0.02;) and nonatopic (21.3 + 5.2 cells/mmý; P 0.01) AT polyps. The number of eosinophils, mast cells, and CD45RO+ cells weer significantly increased in AH compared to AT polyps () 0.001), and the density of HLA-DR+ cells in AH patients was higher than in nonatopic (P 0.02), but not in atopic AT patients. While in AH patients the duration of rhinosinusitis correlated inversely with the number of apoptotic cells (r = - 0.67; P 0.04), in contrast, in AT atopic patients the duration of rhinosinusitis showed positive correlation with apoptosis (r = 0.89; P 0.003). Conclusions: We conclude, that decreased apoptosis of inflammatory cells in nasal polyps from ASA-hypersensitive patients, reflects a distinct mechanism of local inflammation and may be related to persistence and severity of the disease in these patients.


    102/355

    Tytuł oryginału: Eotaxin but not MCP - 3 induces eosinophil influx into nasal fluid in allergic patients.
    Autorzy: Górski P., Wittczak T., Walusiak J., Pałczyński C., Ruta U., Kuna P., Alam R.
    Źródło: Allergy 2002: 57 (6) s.519-528, il., bibliogr. 36 poz.
    Sygnatura GBL: 302,302

    Hasła klasyfikacyjne GBL:
  • immunologia
  • otorynolaryngologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Bacground: Eotaxin and MCP-3 (CC chemokines), owing to their preferential action on eosinophils, seem to be the very importance in the patophysiology of allergic rhinitis and asthma. The purpose of this study was to examine the effect of intranasally administered eotaxin and MCP-3 after specific allergen priming on the influx of inflammatory cells and their soluble mediators into the nasal mucosa. Methods: Eotaxin and MCP-3 have been applied intranasally at the increasing doses of 1, 5 and 10 ćg to allergic patients after priming. The 'nasal pool' technique was used. The cell count and biochemical parameters in nasal lavage were evaluated before 30 min, and 4 and 24 h after the challenge with chemokines. Results: Both eotaxin and MCP-3 induced the increase in clinical 'score' lasting till 24 h. Eosinophil influx into nasal mucosa after provocation with eotaxin was also obsrved. The challenge with MCP-3 did not induce any significant changes in nasal lavage fluid. Conclusions: Eotaxin is likely to play an important role in the pathogenesis of allergic conditions in humans. MCP-3 did not induce inflammatory cell influx into nasal mucosa. The role of this chemokine in the pathogenesis of allergic inflammation is difficult to assess and requires further research.


    103/355

    Tytuł oryginału: Oral cavity as a potential source of gastric reinfection by Helicobacter pylori.
    Autorzy: Karczewska Elżbieta, Konturek Joanna E., Konturek Peter C., Cześnikiewicz Marta, Sito Edward, Bielański Władysław, Kwiecień Nina, Obtułowicz Wojciech, Ziemniak Witold, Majka Jolanta, Hahn Eckhart G., Konturek Stanisław J.
    Źródło: Dig. Dis. Sci. 2002: 47 (5) s.978-986, il., tab., bibliogr. 24 poz.
    Sygnatura GBL: 310,037

    Hasła klasyfikacyjne GBL:
  • stomatologia
  • mikrobiologia
  • gastroenterologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Helicobacter pylori (Hp) is a common pathogen colonizing the a gastric mucosa, but some reports indicated that it may also be found in the oral cavity, which could serve as a reservoir of the bacteria and a source of gastric reinfection. Accordingly, we aimed to study whether the oral cavity, particularly gingival pockets, are colonized by Hp and whether it could be the source of gastric reinfection. We studied 329 patients with dyspeptic symptoms (257 with chronic gastritis, 15 with gastric ulcer. and 57 with duodenal ulcer). The [**13C]urea breath test (UBT), gastroscopy, and Hp culture from gastric biopsies were carried out, and material was collected from the oral cavity (gingival pocket) for bacteriological culture and genomic DNA studies. The serum was obtained for anti-Hp IgG and anti-CagA assays and saliva for anti-Hp IgA determination using the ELISA technique. bacteria in material from gingival pockets and biopsies from the corpus and antrum of stomach of 30 DU patients before and after Hp eradication were also examined by PCR technique, using primers specific for 16S rNA. All Hp-positive patients (276) were subjected to one week of triple therapy (omeprazole 2 x 20 mg twice a day, clarithromycin 2 = 500 mg twice a day, and metronidazole 2 x 500 mg twice a day). The measurements descrived above were then repeated at four weeks and six months. Bacterkilogical culture showed the prsence of Hp in the material from oral cavity in about 50 p.c. of patients, whereas UBT, used as a gold standard, revealed gastric Hp infection in about 84 p.c. of these patients...


    104/355

    Tytuł oryginału: Functionally impaired TR mutants are present in thyroid papillary cancer.
    Autorzy: Puzianowska-Kuźnicka Monika, Krystyniak Agnieszka, Madej Agnieszka, Cheng Sheue-Yann, Nauman Janusz
    Źródło: J. Clin. Endocrinol. Metab. 2002: 87 (3) s.1120-1128, il., tab., bibliogr. 52 poz.
    Sygnatura GBL: 310,620

    Hasła klasyfikacyjne GBL:
  • genetyka
  • endokrynologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: TRs are transcription factors that regulate cell proliferation, differentiation, and apoptosis. They are cellular homologs of the transcriptionally inactive viral oncogene v-erbA. We tested the hypothesis that the functions of TRs could be impaired in cancer tissues as a result of aberrant expression and/or somatic mutations. As a model system, we selected human thyroid papillary cancer, in which the most common abnormalities, RET/papillary thyroid cancer rearrangements (fusion of RET kinase domain to the activating domains of other genes), were found in 40-45 p.c. of cases. We found that the mean expression levels of TRá mRNA and TRŕ mRNA were significantly lower, whereas the protein levels of TRá1 and TRŕ1 were in cancer tissues than in healthy thyroid. Sequencing of TRá1 and TRŕ1 cDNAs, cloned from 16 papillary cancers, revealed that mutations affected receptor amino acid sequences in 93.75 p.c. and 62.5 p.c. of cases, respectively. In contrast, no mutations were found in healthy thyroid controls, and only 11.11 p.c. and 22.22 p.c. of thyroid adenomas had such TRá1 or TRŕ1 mutations, respectively. The majority of the mutated TRs lost their trans-activation function and exhibited dominant negative activity. These findings suggest a possible role for mutated thyroid hormone receptors in the tumorigenesis of human papillary thyroid carcinoma.


    105/355

    Tytuł oryginału: Salvage hemipelvis radiotherapy with fertility preservation in an adolescent with recurrent vulvar carcinoma.
    Autorzy: Serkies Krystyna, Wysocka Barbara, Emerich Janusz, Hrabowska Maria, Jassem Jacek
    Źródło: Gynecol. Oncol. 2002: 85 (2) s.381-383, bibliogr. 10 poz.
    Sygnatura GBL: 305,822

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo
  • onkologia
  • psychiatria i psychologia

    Typ dokumentu:
  • praca kazuistyczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Background. To preserve fertility, hemi-pelvis irradiation was chosen for locoregional groin recurrence of vulvar cancer in an adolescent. Case. A case of squamous cell vulvar carcinoma in uncommonly young patient is presented. First surgical management (local excision of T1N0M0 tumor of the right labia minora) was performed at the age of 16.5 years. Further therapy included wide local resection of recurrent local lesion, two subsequent ipsilateral groin dissections of nodal metastases (with extranodal spread at the first instance), and ipsilateral pelvic lymphadenectomy. The patient was administered external beam irradiation of 45 Gy to the hemipelvis followed by 10.8 Gy boost to the right inguinal region. After 6.5 years from the completion of radiotherapy the patients is free of disease. She managed to conceive but the labor was premature and the infant died in its 7th week pf life. Conclusion. In this case salvage hemi-pelvic irradiation for groin metastases of vulvar cancer has proved to be an effective treatment, allowing preservation of hormonal and obstetric functions. Partial post-irradiation damage of the uterus might have caused premature labor. Thus, special obstetric care is advisable in such situations.


    106/355

    Tytuł oryginału: Expression of the multidrug resistance-associated protein (mrp) gene in chronic lymphocytic leukemia.
    Autorzy: Juszczyński Przemysław, Niewiarowski Wojciech, Krykowski Euzebiusz, Robak Tadeusz, Warzocha Krzysztof
    Źródło: Leuk. Lymphoma 2002: 43 (1) s.153-158, il., tab., bibliogr. 37 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • genetyka
  • farmacja
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: In order to define more accurately the role of multidrug resistance (MDR)-related protein (mrp) gene in chronic lymphocytic leukemia (CLL), we addressed the question of its expression pattern in isolated peripheral blood B lymphocytes in seven healthy donors and 28 patients with CLL, with respect to some laboratory and clinical parameters of the disease. For this purpose, we used a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), based on the coamplification of an internal standard not homologous to the DNA target to quantify the mrp transcription level in each studied sample. We report that the level of constitutive mrp gene's expression in peripheral blood lymphocytes is higher in CLL patients than in healthy controls. We found increased mrp gene expression levels in patients with higher white blood cells (WBC) and lymphocytes' counts as well as in more advanced disease stages according to Rai or Binet scale. Finally, mrp gene's expression was higher in patients with progressive CLL, especially in cases refractory to chemotherapy salvage. The results of the present study suggest that expression of mrp gene might be relevant in the pathogenesis of the MDR phenotype in CLL.


    107/355

    Tytuł oryginału: The role of nucleoside analogues in the treatment of chronic lymphocytic leukemia-lessons learned from prospective randomized trials.
    Autorzy: Robak Tadeusz
    Źródło: Leuk. Lymphoma 2002: 43 (3) s.537-548, il., tab., bibliogr. 78 poz.
    Sygnatura GBL: 312,939

    Hasła klasyfikacyjne GBL:
  • farmacja
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The newer purine nucleoside analogues (PNA), fludarabine (FAMP) and cladribine (2-chlorodeox-yadenosine, 2-CdA) have been synthesized recently and introduced into the treatment of chronic lymphocytic leukemia (CLL). The results of large phase II studies indicate that FAMP and 2-CdA are similarly active in CLL. Unfortunately, no prospective randomized study comparing the results of the treatment of CLL patients with FAMP and 2-CdA has been published so far. Significantly higher overall response (OR) and complete remission (CR) in patients treated initially with PNA than with chlorambucil or cyclophosphamide based combination regimens has been recently confirmed in five prospective multicentre randomized trials. These studies have also shown longer response duration in patients treated with PAN than with conventional chemotherapy. Overall survival progression free and events free survival were similar in patients treated with ONA and with chlorambucil or other alkylating agent based regimens. However, the majority of randomized trials were designed as cross studies and most patients, treated with conventional chemotherapy were given PNA when refractory or in early relapse, which may influence the survival time. The results of a randomized study have shown a higher incidence of neutropenia and infections in patients treated with PNA than with chlorambucil. However, the frequency of autoimmune hemolytic anemia, pure red cell aplasia, secondary neoplasms and Richter's syndrome seems to be similar in patients treated with PNA and standard alkylating agents based chemotherapy...


    108/355

    Tytuł oryginału: Intercalation of imidazoacridinones to DNA and its relevance to cytotoxic and antitumor activity.
    Autorzy: Dzięgielewski Jarosław, Ślusarski Bartłomiej, Konitz Antoni, Składanowski Andrzej, Konopa Jerzy
    Źródło: Biochem. Pharmacol. 2002: 63 (9) s.1653-1662, il., tab., bibliogr. 33 poz.
    Sygnatura GBL: 304,395

    Hasła klasyfikacyjne GBL:
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Imidazoacridinones (IA) are a class of antitumor agents which includes C-1311, an interesting drug in clinical trials. This study investigated the mechanism of IA binding to DNA for a series of 13 analogs that differ in their cytotoxic potency. Using C-1311 as a model compound, crystallographic, spectroscopic and biochemical techniques were employed to characterize drug-DNA interactions. X-ray crystallographic analysis revealed a planar structure of imidazoacridinone core that is capable of intercalative DNA bibding. Accordingly, C-1311 binding to DNA followede "classical" pattern observed for intercalation, as proved by tha DNA topoisomerase I - unwinding experiments, with relatively weak binding affinity (Ki = 1.2 x 10**5 M-1), and the binding site size of 2.4 bp. Other IA also bound to DNA with the binding affinity in the range of 10**5 M-1 and binding site size of 2-3 bp, suggesting a prevalence of the intercalative mechanism, similar to C-1311. Considerable DNA binding affinity was displayed by all highly cytotoxic derivatives. However, none of the analyzed drug-DNA binding parameters was significantly correlated with IA biological activities such as cell growth. DNA and RNA synthesis inhibition, or tumor growth inhibition, which suggests that the IA ability to non-covalently bind to DNA is not crucial for their biological activity. These results show that the ability to intercalate into DNA is a prominent attribute of IA, although factors other intercalative binding seem to be required for the biological activities of IA drugs.


    109/355

    Tytuł oryginału: Inhibition of gluconeogenesis by vanadium and metformin in kidney-cortex tubules isolated from control and diabetic rabbits.
    Autorzy: Kiersztan Anna, Modzelewska Aleksandra, Jarzyna Robert, Jagielska Elżbieta, Bryła Jadwiga
    Źródło: Biochem. Pharmacol. 2002: 63 (7) s.1371-1382, il., tab., bibliogr. 75 poz.
    Sygnatura GBL: 304,395

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • farmacja
  • nefrologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • króliki

    Streszczenie angielskie: Effect of vanadyl acetylacetonate (VAc) and metformin on gluconeogenesis has been studied in isolated hepatocytes and kidney-cortex tubules of rabbit. Glucose formation from alanine + glycerol + octanoate, pyruvate or dihydroxyacetone was inhibited by 50-80 p.c. by 100 ćM VAc or 500 ćM metformin in renal tubules of control and alloxan-diabetic animals, while the inhibitory action of these compounds in hepatocytes was less pronounced (by about 20-30 p.c.). In contrast to VAc, metaformin increased the rate of lactate formation by about 2-fold in renal tubules incubatet with alanine + glycerol + octanoate. In view of VAc-induced changes in intracellular gluconeogenic intermediates and gluconeogenic enzyme activities, it is likely taht this compound may decrease fluxes through pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase. In contrast to VAc, metformin-induced decrease in renal gluconeogenesis may result from a decline of cytosolic oxaloacetate level and consequently PEPCK activity. Following 6 days of VAc administration (1.275 mg V kg-1 body weight daily) the blood glucose level in alloxan-diabetic rabbits was normalised while blood glucose changes in control animals were not observed. On the contrary, in diabetic animals treated for 6 days with metformin (200 mg kg-1 body weight day -1) a high blood glucose level was maintained. Unfortunately, VAc-treated control and diabetic rabbits exhibited elevated serum urea and creatinine levels. In VAc-treated animals vanadium was accumulated in kidney=cortex up to 7.6 ń 0.6 ćg V g-1 dry weight. In view of a potential vanadium nephrotoxicity...


    110/355

    Tytuł oryginału: Testing for aspirin hypersensitivity.
    Autorzy: Bochenek G., Niżankowska E[wa], Szczeklik A[ndrzej]
    Źródło: Allergy 2002: 57 (7) s.562-565, bibliogr. 33 poz.
    Sygnatura GBL: 302,302

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • immunologia
  • otorynolaryngologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    111/355

    Tytuł oryginału: The immunophenotype of patients with recurrent pregnancy loss.
    Autorzy: Darmochwał-Kolarz Dorota, Leszczyńska-Gorzelak Bożena, Roliński Jacek, Oleszczuk Jan
    Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 103 (1) s.53-57, tab., bibliogr. 17 poz.
    Sygnatura GBL: 305,582

    Hasła klasyfikacyjne GBL:
  • immunologia
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Objective: The aim of the study was to estimate the alterations in the phenotype of lymphocytes of women with unexplained pregnancy failures in comparison with healthy women. Materials and methods: Fourten women with unexplained habitual miscarriages and 18 healthy, fertile women with the history of successful pregnancies were included in the study. The lymphocytes were isolated from peripheral blood and stained with monoclonal antibodies. The expression of selected surface molecules was estimated using the flow cytometric method. Results: We found that the percentage of T CD4+ lymphocytes, CD3-16/56+ cells, and T CD8+11b- cells was significantly higher in patients with recurrent pregnancy loss in comparison with healthy women. The peercentage of B-1 CD19+5+ lymphocytes was also significantly higher in women with unexplained habitual miscarriages in comparison with healthy women. Furthermore, we found higher expression of CD25 molecule on T CD3+ and T CD4+ lymphocytes in the study group, when compared to controls. Moreover, the percentages of B CD19+ and T suppressor CD8+11b+ lymphocytes were lower in women with pregnancy failures in comparison with the control group. The percentage of T CD3+ lymphocytes and T CD8+ cells did not differ in both studied groups. Similarly, the expression of CD25 antigen and HLA-DR molecule on T CD8+ did not differ in the study group, when compared to controls. Concvlusion: Our results can suggest that immunological alterations may be involved in the etiopathogenesis of unexplained recurrent pregnancy loss.


    112/355

    Tytuł oryginału: Direct antimicrobial properties of substance P.
    Autorzy: Kowalska Katarzyna, Carr Daniel B., Lipkowski Andrzej W.
    Źródło: Life Sci. 2002: 71 (7) s.747-750, il., tab., buibliogr. 14 poz.
    Sygnatura GBL: 304,948

    Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: The structural similarity between substance P (SP, Arg-Pro-Lys-Pro-Gln-Gln-Phe-Gly-Leu-Met-NH2) and Arg/Pro rich bactericidal peptides suggests a possible direct effect of SP on invasive microbes. We now present evidence that substance P possesses direct antimicrobial activity, highest against S. aureus. A substance P antagonist allso possess such activity but while less potent than substance P agonist S. aureus, is more potent than substance P against C. albicans. Our data also show that the endogenous peptides bradykinin and neurotensin, that also play role in modulation of the host-defense system in situ, have antimicrobial properties but are less potent than substance P.


    113/355

    Tytuł oryginału: New renin inhibitors with pseudodipeptidic units in P1-P1' and P2'-P3' positions.
    Autorzy: Paruszewski Ryszard, Jaworski Paweł, Winiecka Iwona, Tautt Jadwiga, Dudkiewicz Jadwiga
    Źródło: Chem. Pharm. Bull. (Tokyo) 2002: 50 (6) s.850-853, tab., bibliogr. 21 poz.
    Sygnatura GBL: 304,601

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: A series of four new potential renin inhibitors has been synthesized. The structure of the compounds was designed in such a way as to produce agents resistant to enzymatic degradation, metabolically stable, possibly potent and with improved oral absorption. All positions of the 8-13 fragment of the human angiotensinogen were occupied by unnatural units (two unnatural amino acids in position P3 and P2 and two pseudodipeptides in positions P1-P1, and P2-P3,). Both N- and C-terminal functions of the inhibitors were blocked with tert-Boc and ethyl ester groups. Their hydrophobicity evaluated as a log P value, calculated by a computer method, was 6.57 and 6.08 respectively. All peptides were obtained by the carbodiimide method in solution and purified by chromatography on the SiO2 column. Their resistance to enzymatic degradation was assayed by determination of stability against chymotrypsin activity. The potency was measured in vitro by a spectrofluorimetric method (assay of Leu-Val-Tyr-Ser released from the N-acetyltreadecapeptide substrate by renin in the presence of the inhibitor). All inhibitors were stable to chymotrypsin. Their IC50 (M/I) values were: 9.6 x 10**-4 (12), 1.6 x 10**-5 (17), 1.0 X 10**-5 (22) and 1.0 x 10**-5 (23) respectively.


    114/355

    Tytuł oryginału: Myocardial ischemia possibly mediated by cysteinyl leukotrienes.
    Autorzy: Szczeklik Andrew, Niżankowska Ewa, Mastalerz Lucyna, Bochenek Grażyna
    Źródło: J. Allergy Clin. Immunol. 2002: 109 (3) s.572-573, tab., bibliogr. 8 poz.
    Sygnatura GBL: 301,408

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kazuistyczna
  • praca opublikowana za granicą
  • list i notatka
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć męska


    115/355

    Tytuł oryginału: PRESTO (a mammoth trial that could have been prevented), HPS and REMATCH.
    Autorzy: Doehner Wolfram, Ponikowski Piotr, Anker Stefan D.
    Źródło: Int. J. Cardiol. 2002: 83 (2) s.99-102, bibliogr. 17 poz. - 74 Doroczne Spotkanie Towarzystwa Chorób Serca Anaheim 11. 2001
    Sygnatura GBL: 305,693

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    116/355

    Tytuł oryginału: Myocardial ischemia and autonomic activity in dippers and non-dippers with coronary artery disease: assessment of normotensive and hypertensive patients.
    Autorzy: Kurpesa Małgorzata, Trzos Ewa, Drożdż Jarosław, Bednarkiewicz Zbigniew, Krzemińska-Pakuła Maria
    Źródło: Int. J. Cardiol. 2002: 83 (2) s.133-142, il., tab., bibliogr. 36 poz.
    Sygnatura GBL: 305,693

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: The aim of this study was to assess the relations between the circadian variations of blood pressure (BP) and the pattern of ischemia and autonomic activity in normotensive and hypertensive patients with coronary artery disease (CAD). Patients and methods: On the basis of the results of ambulatory BP monitoring, 115 patients with stable CAD were divided into Group 1 (with arterial hypertension) and Group 2 (normotensives). Groups were subdivided into dippers and non-dippers. Holter monitoring was performed to assess the occurrence and circadian pattern of ischemic episodes. Time domain and frequency domain HRV analyses were performed to evaluate the autonomic activity. Results: The total number of ischemic episodes was similar in dippers and non-dippers. Non-dippers had a greater number of silent episodes and a different circadian pattern of ischemia with more night episodes. Among the time-domain HRV parametres, only SDNN was similar in dippers and non-dippers. Non-dippers had lower pNN50 and rMSSD - the parameters expressing parasympathetic activity. Differences between diurnal and nocturnal results of spectral HRV analysis were observed in dipper patients only. They presented an elevation of HF power and a decline of LF power at night. All differences between dippers and non-dippers were of similar significance in both hypertensives and normotensives. Conclusions: A lack of a nocturnal fall in BP is present in normotensive and hypertensive patients with CAD. Non-dippers with CAD had silent and nightime ischemia more often. They also had an abnormal pattern of autonomic activity with higher sympathetic and lower parasympathetic modulation.


    117/355

    Tytuł oryginału: Serum levels of selected adhesion molecules in patients with coronary artery disease.
    Autorzy: Mizia-Stec Katarzyna, Zahorska-Markiewicz Barbara, Mandecki Tadeusz, Janowska Joanna, Szulc Andrzej, Jastrzębska-Maj Ewa
    Źródło: Int. J. Cardiol. 2002: 83 (2) s.143-150, tab., bibliogr. 32 poz.
    Sygnatura GBL: 305,693

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • badanie porównawcze
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Background: Adhesion molecules have been suggested as mediators of atherosclerotic inflammatory process. They may contribute to the pathogenesis of stable and unstable angina. Methods: The study group consisted of 59 patients with coronary artery disease: 27 patients with stable exertional angina (group A), 32 patients with unstable angina (group B). 20 healthy persons acted as controls (group C). Serum levels of soluble Intercellular Adhesion Molecule 1 (sICAM-1), Vascular Cell Adhesion Molecule 1 (sVCAM-1), sE-selectin, sP-selectin were measured both before and after the treadmill ECG stress test in groups A and C. In group B the measurements were carried out at 6, 24, and 48 hours following an episode of chest pain. Results: There were no differences between the baseline serum levels of adhesion molecules as determined in groups A and C. In patients with stable angina, the post-exercise concentrations of sE-selectin were significantly higher (68.8 ń 29 ng/ml) in comparison to both baseline- (38.7 ń 15 ng/ml), and group C-values (pre-exercise: 35.1 ń 16; post-exercise: 49.9 ń 15 ng/ml). In unstable patients, serum sP-selectin (190.1 ń 99 ng/ml) and sVCAM-1 levels (1359 ń 299 ng/ml) were higher when compared to those found in groups A (142.3 ń 24; 962 ń352 ng/ml, respectively) and C (136.4 ń 33; 851 ń 168 ng/ml, respectively). Conclusions: Serum levels of soluble adhesion molecules in patients with stable angina are comparable to those of healthy persons. Stress test-induced increase of sE-selectin concentration may reflect endothelial response to exercise. Unstable angina is characterized by significant elevation of sP-selectin and sVCAM-1 serum levels which seems to be related to enhanced platelets and leukocytes activation.


    118/355

    Tytuł oryginału: Atrial natriuretic peptide level after cardioversion of chronic atrial fibrillation.
    Autorzy: Wozakowska-Kapłon Beata, Opolski Grzegorz
    Źródło: Int. J. Cardiol. 2002: 83 (2) s.159-165, il., tab., bibliogr. 28 poz.
    Sygnatura GBL: 305,693

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Heart endocrine studies concerning patients with chronic atrial fibrillation (AF) have become increasingly important. Atrial natriuretic peptide (ANP) is released from atrial myocytes. The increased level of ANP in patients with AF is probably caused by the hemodynamic effect of the arrhythmia. The aim of this study was to explore plasma ANP levels in patients with chronic AF and to describe plasma ANP concentration changes following sinus rhythm (SR) restoration. The study group was comprised of 42 patients, aged between 43 and 76 years with chronic ASF (more than 1 month) and a relatively controlled ventricular response (85.8 ń 11.3 beats/min). Plasma ANP levels were measured before and 24 h after AF cardioversion. The control group comprised of 11 subjects. All had normal SR without history of AF and were compatible in age, sex and concomitant diseases with the examined group. ANP level values were expressed as mean ń standard deviation. The mean plasma ANP level in the AF group was significantly higher than in the control group (59.5 ń 16.6 vs. 34.3 ń 10.2 pg/ml, P 0,001). Electrical or pharmacological cardioversion was performed in 42 patients. SR was succesfully restored in 35 patients. Plasma ANP concentrations decreased significantly from baseline values (from 59.4 ń 16.6 to 31.4 ń 15.0 pg/ml, P 0.001) 24 h after cardioversion in the successful group, while they remained unchanged (60.2 ń 10.7 to 59.4 ń 10.4 pg/ml, NS) in patients with an unsuccessful cardioversion. Conclusion: The mean concentration of ANP in patients with chronic AF was nearly two-times higher than in the control group with sinus rhythm. Conversion to SR was associated with a significant decrease and normalization in plasma ANP concentrations.


    119/355

    Tytuł oryginału: Inhibitory effect of acetylsalicylic acid on metalloproteinase activity in human lung adenocarcinoma at different stages of differentiation.
    Autorzy: Karna Ewa, Pałka Jerzy A.
    Źródło: Eur. J. Pharmacol. 2002: 443 (1/3) s.1-6, il., tab., bibliogr. s. 5-6
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja
  • pulmonologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: The mechanism underlying the anticancer effect of nonsteroidal anti-inflammatory drugs (NSAIDs) is not clear. We adressed the question whether the alterations in collagen content in lung adenocarcinomas reported in previous studies result from dysregulation of gelationylytic activity and whether the activity is altered by acetylsalicylic acid in vitro. Human lung adenocarcinomas were divided into three groups: well-differentiated (G1), moderately differentiated (G2) and poorly differentiated (G3) tumors. Each group was compared with normal lung tissue with respect to tissue collagean and collagen degradation product content (hydroxyproline assay), gelatinolytic activity (zymography) and the expression of matrix metalloproteinases, MMP-2 and MMP-9 (Western immunoblot). Moreover, in the studied tissues, the effect of acetylsalicylic acaid on gelatinolytic activity was measured. The lung adenocarcinoma G1 had a similar collagen content as normal lung tissue but increased amounts of collagen degradation products and free hydroxyproline. These phenomena were accompanied by a marked increase in gelatinolytic activity (NMP-2 and MMP-9) in the G1 tumor. In adenocarcinoma G2, the free hydroxyproline content and gelatinolytic activity were increased, while the collagen and collagen degradation product contents were not markedly altered, compared to control. In contrast, adenocarcinoma G3 had an increased tissue collagen content (by about 60 p.c.), decreased percentage of collagen degradation products and similar gelatinolytic activity, compared to normal lung. Acetylsalicylic acid...


    120/355

    Tytuł oryginału: Imipramine but not 5-HT1A receptor agonists or neuroleptics induces adaptive changes in hippocampal 5-HT1A and 5-HT4 receptors.
    Autorzy: Zahorodna Agnieszka, Tokarski Krzysztof, Bijak Maria
    Źródło: Eur. J. Pharmacol. 2002: 443 (1/3) s.51-57, il., bibliogr. s. 56-57
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • badanie porównawcze
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: It has been reported that the treatment with a tricyclic antidepressant imipramine induces an increase in the sensitivity of 5-HT1A receptors and a decrease in the sensitivity of 5-HT4 receptors in the rat hippocampus. 5-HT1A receptor agonists and neuroleptics also affect 5-HT1A receptors in different brain areas; therefore, it was of interest to compare their effects on hippocampal 5-HT receptors with the influence of the well-established antidepressant imipramine. We studied the effects of repeated treatment with imipramine, the 5-HT1A receptor agonists 8-hydroxy-2(di-n-propylamino)tetralin (8-OH-DPAT) and buspirone, and the neuroleptics haloperidol and clozapine on the sensitivity of rat hippocampal CA1 neurons to 5-HT1A - and 5-HT4 receptor activation. Imipramine was administered for 21 days (10 mg/kg p.o., twice daily), 8-OH-DPAT for 7 days (1 mg/kg s.c., twice daily) and buspirone for 21 days (5 mg/kg s.c., twice daily). The rats received haloperidol (1 mg/kg) and clozapine (30 mg/kg) for 6 weeks in drinking water. Hippocampal slices were prepared 2 days after the last treatment with imipramien, 8-OH-DPAT or buspirone, and 5 days after the last treatment with the neuroleptics. Using an extracelllar in vitro recording, we studied changes in the amplitude of stimulation-evoked population spikes, induced by 5-HT, 8-OH-DPAT and the 5-HT4 receptor agonist zacopride. Activation of 5-HT1A receptors decreased, while activation of 5-HT4 receptors increased the amplitude of population spikes. Imipramine significatly enhanced the inhibitory effects of 5-HT and 8-OH-DPAT, and attenuated...


    121/355

    Tytuł oryginału: Circulating E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in men with coronary artery disease assessed by angiography and disturbances of carbohydrate metabolism.
    Autorzy: Kowalska Irina, Strączkowski Marek, Szelachowska Małgorzata, Kinalska Ida, Prokop Jolanta, Bachórzewska-Gajewska Hanna, Stępień Agnieszka
    Źródło: Metabolism 2002: 51 (6) s.733-736, tab., bibliogr. 22 poz.
    Sygnatura GBL: 303,583

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć męska

    Streszczenie angielskie: It is hypothesized that adhesion molecules could be an early predictor of coronary artery diseases. Therefore we investigated the relationship between the concentrations of soluble forms of adhesion molecules and disturbances of glucose metabololism in 78 men referred for coronary angiography but with no previous history of diabetes. The group consisted of 78 men (mean age, 47.6 ń 7.0 years; mean body mass index [BMI], 28.4 ń 3.24 with the symptoms of angina pectoris and positive exercise test. All subjects were given a standard oral glucose tolerance test (OGTT) with glucose and insulin estimations. Fasting plasma concentrations of the soluble (s) forms of E-selectin, intercelular adhesion cell molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), and HbA1c were also measured. According to the OGTT, 10.2 p.c. of the patients (n = 8) fulfilled the criteria for type 2 diabetes mellitus and 44.9 p.c. (n = 35) for impaired glucose tolerance (IGT). The highest concentrations of sE-selectin were observed in patients with type 2 diabetes mellitus and were significantly higher in comparison to the group with normal glucose tolerance and IGT. The concentration of sVCAM-1 increased with the progression of disturbances of glucose metabolism and remained the highest in type 2 diabetic patients. sICAM-1 concentration was not significantly different. sE-selectin concentration correlated significantly with fasting glucose (r = 0.23, P = .041), postload glucose (r = 0.39, P = .001), and postload...


    122/355

    Tytuł oryginału: Increased rate of cholesterologenesis - a possible cause of hypercholesterolemia in experimental chronic renal failure in rats.
    Autorzy: Szołkiewicz M[arek], Sucajtys E., Chmielewski M., Wołyniec W., Rutkowski P., Bogusławski W., Świerczyński J., Rutkowski B[olesław]
    Źródło: Horm. Metab. Res. 2002: 34 (5) s.234-237, il., tab., bibliogr. 19 poz.
    Sygnatura GBL: 312,226

    Hasła klasyfikacyjne GBL:
  • genetyka
  • nefrologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć męska

    Streszczenie angielskie: Hipercholesterolemia plays an important role in the lipid abnormalities in chronic renal failure (CRF). It is thourght to contribute to both a progression of renal failure and atherosclerosis. Despite intensive research, the etiopathogenesis of hypercholesterolemia in CRF patients is still obscure. The present sudy was designed to evaluate the possible role of cholsterol overproduction in the development of hypercholesterolemia associated with experimental CRF. We found that plasma total cholesterol and cholesterol distributed in VLDL, LDL and HDL concentrations were significantly enhanced in CRF rats. Simultaneously, the rate of liver cholesterol biosynthesis in vivo (measured by determining the incorporation of tritium from tritiated water intraperiotoneally injected into cholesterol), liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity and liver HMG-CoA reductase mRNA presence were elevated. Significant increases in activity of liver malic enzyme, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase NADPH-producing enzyme (required for cholesterol synthesis) have also been observed in CRF rats. In conclusion, the increased rate of liver cholesterol biosynthesis due to increase of HMG-CoA reductase and NADPH-producing enzyme gene expression could be one of the possible causes of hypercholesterolemia in CRF animals.


    123/355

    Tytuł oryginału: Knockdown of spinal opioid receptors by antisense targeting á-arrestin reduces morphine tolerance and allodynia in rat.
    Autorzy: Przewłocka Barbara, Sieja Agnieszka, Starowicz Katarzyna, Maj Marcin, Bilecki Wiktor, Przewłocki Ryszard
    Źródło: Neurosci. Lett. 2002: 325 (2) s.107-110, il., bibliogr. 13 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • badanie porównawcze
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The development of morphine tolerance and sciatic nerve injury-induced allodynia after functional knockdown of spinal opioid receptors using antisense oligonucleotides targetin á-arrestin was investigated. Ineffectiveness of morphine in neuropathic pain suggests an implication of the same mechanism in these two processes. The development of morphine tolerance (10 ćg intrathecally (i.th.), every 12 h) was significantly inhibited in rats, which received i.th. á-arrestin antisenses (2 nM). Acute and chronic (6 days) i.th. administration of antisenses antagonized the allodynia in the rat model of neuropathic pain. Our results demonstrated that i.th. administration of á-arrestin antisenses delayed development of tolerance to morphine and nerve injury-induced cold allodynia, which suggest that both of the investigated phenomena may be mediated by a similar mechanism, e.g. receptor desensitization. Moreover, the antisense oligonucleotides targeting á-arrestin may constitute a new approach to the therapy of neuropathic pain.


    124/355

    Tytuł oryginału: A modified neutral comet assay: elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody.
    Autorzy: Wojewódzka Maria, Buraczewska Iwona, Kruszewski Marcin
    Źródło: Mutat. Res 2002: 518 (1) s.9-20, il., bibliogr. 43 poz.
    Sygnatura GBL: 304,952

    Hasła klasyfikacyjne GBL:
  • genetyka

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Comet assay under neutral conditions allows the detection of DNA double-strand breaks (DSB), considered to be the biologically relevant radiation-induced lesion. In this report, we describe modifications of the neutral comet method, which simplify and facilitate its use for estimation of DNA DSB in X-irradiation mammalian cells in centure. The analysis carried out according to this protocol takes less time than those most often applied. Also, the use of lysis at 50ř C is avoided; this is important in view of the presence of heat-labile sites in the chromatin of irradiated cells, recently reported by Rydberg [Radiation-induced heat-labile sites that convert into DNA double-strtand breaks, Radsaiation Research 153 (2000) 805-812]. The comets have well-defined, sharp limits, suitable for image analysis. The chromatin of the hydrogen peroxide-treated or UV-C-irradiated cell remains condensed similarly to that of the control cells. We checked the neutral comets for the presence of single-stranded DNA by means of a specific antibody. The results point to a satisfactory sensitivity of the modified neutral comet assay and its specificity for DSB. The minimum detection level of the modified neutral comet assay is about 5 Gy.


    125/355

    Tytuł oryginału: Countrywide spread of CTX-M-3 extended-spectrum á-lactamase-producing microorganisms of the family Enterobacteriaceae in Poland.
    Autorzy: Baraniak Anna, Fiett Janusz, Sulikowska Agnieszka, Hryniewicz Waleria, Gniadkowski Marek
    Źródło: Antimicrob. Agents Chemother. 2002: 46 (1) s.151-159, tab., bibliogr. 42 poz.
    Sygnatura GBL: 305,849

    Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Eighty-four clinical isolates of the family Enterobacteriaceae from 1998 to 2000 in 15 hospitals in 10 polish cites, were analyzed. All the isolates produced á-lactamases with pIs of 8.4 and 5.4, and the pI 8.4 enzymes were demonstrated to hydrolyze cefotaxime but not ceftazidime in the in vitro bioassay. PCR analysis and DNA sequencing have revealed that in all cases the pI 8.4 á-lactamase was probably the CTX-M-3 extended-spectrum á-lactamase (ESBL) variant, which was originally identified in 1996 in Praski Hospital in Warsaw. In the majority of isolates, blaCTX-M-3 genes resided large conjugative plasmids with similar fingerprints, which, in the contex of the high degree of diversity of the randomly amplified polymorphic DNA types of the isolates, suggest that horizontal transfer of plasmids was likely the main mechanism of CTX-M-3 spread. The dissemination of plasmids was probably preceded by the center-to-center transmission of several strains, as indicated by the identification by pulsed-field gel electrophoresis of closely related or possibly related Klebsiella pneumoniae, Eschrichia coli, and Citrobacter freundii isolates in five different hospitals. CTX-M-3-producing organisms revealed a very high degree of diversity in á-lactam resistance levels and patterns. This was attributed to several factors, such as the production of other á-lactamases including additional ESBLs, possible quantitative variations in CTX-M-3 expression, segregation of AmpC derepressed mutants, and permeability alterations.


    126/355

    Tytuł oryginału: A randomized, double-blind trial of the effect of treatment with montelukast on bronchial hyperresponsiveness and serum eosinophilic cationic protein (ECP), soluble interleukin 2 receptor (sIL-2R), IL-4, and soluble intercellular adhesion molecule 1 (sICAM-1) in children with asthma.
    Autorzy: Stelmach Iwona, Jerzyńska Joanna, Kuna Piotr
    Źródło: J. Allergy Clin. Immunol. 2002: 109 (2) s.257-263, il., tab., bibliogr. 43 poz.
    Sygnatura GBL: 301,408

    Hasła klasyfikacyjne GBL:
  • pediatria
  • farmacja
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.

    Streszczenie angielskie: Background: Anti-inflammatory properties of leukotriene modifiers and their on bronchial hyperresponsiveness have npt been studied in children with asthma. Objective: The primary objective of this study was to determine the changes in serum levels of inflammatory mediators, clinical efficacy, and bronchial hyperresponsiveness after treatment with montelukast. Methods: In this double-blind, randomized, placebo-controlled trial, 39 children with mild-to-moderate atopic asthma were randomly allocated to receive montelukast or placebo or 6 weeks. Main outcome measures were changes in serum concentrations of soluble interleukin 2 receptor (sIL-2R), IL-4, and soluble intercellular adhesion molecule 1 (sICAM-1); peripheral blood eosinophil count; and eosinophilic cationic protein (ECP). Asthma severity score, FEV1, and bronchial hyperreactivity (BHR) for histamine were secondary end points. Results: Compared to placebo, serum concentrations of IL-4 sICAM-1, and ECP and eosinophil blood counts significantly decreased after 6 weeks of treatment with montelukast. Montelukast significantly improved asthma control and FEV1. Montelukast resulted in within-group significant decrease in levels of serum sIL-2R (611 vs 483 pg/mL), IL-4 (0.123 vs 0.102 pg/mL), sICAM-1 (280 vs 244 ng/mL), and ECP (74 vs 59 ćg/mL) and in eosinophil blood counts (349 vs 310 cells/mm3). Mean FEV1 value changed from 85 p.c. of predicted to 95 p.c. (P .001) and for histamine (PC20H) from 2.8 mg/L to 3.8 mg/mL (P .001) after treatment with montelukast...


    127/355

    Tytuł oryginału: Lovastatin potentiates antitumor activity of doxorubicin in murine melanoma via an apoptosis-dependent mechanism.
    Autorzy: Feleszko Wojciech, Młynarczuk Izabela, Olszewska Dominika, Jalili Ahmad, Grzela Tomasz, Lasek Witold, Hoser Grażyna, Korczak-Kowalska Grażyna, Jakóbisiak Marek
    Źródło: Int. J. Cancer 2002: 100 (1) s.111-118, il., tab., bibliogr. 37 poz.
    Sygnatura GBL: 305,029

    Hasła klasyfikacyjne GBL:
  • farmacja
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • zwierzęta

    Streszczenie angielskie: Lovastatin, a drug successfully used in the clinic to prevent and to treat coronary heart disease, has recently been reported to decrease the incidence of melanoma in lovastatintreated patients. Lovastatin has also been proved to potentiate antitumor effects of both cisplatin and TNF-ŕ in murine melanoma models. Recently, an augmented therapeutic effect of lovastatin and doxorubicin has been reported in 3 tumor models in mice. In our preliminary study lovastatin caused retardation of melanoma growth in mice treated with doxorubicin (Feleszko et al. J. Nati Cancer Inst 1998;90:247-8). In the present report, we supplement our preliminary observations and demonstrate in 2 murine and 2 human melanoma cell lines that lovastatin effectively potentiates the cytostatic/cytotoxic activity of doxorubicin in vitro via an augmentation of apoptosis (estimated with PARP-cleavage assay, annexin V assay and TUNEL). The combined antiproliferative activity of lovastatin and doxorubicin was evaluated using the combination index (CI) method of Chou and Talalay, revealing synergistic interactions in melanoma cells exposed to lovastatin and doxorubicin. In BI6FI0 murine melanoma model in vivo, we have demonstrate significantly increased sensitivity to the combined treatment with both lovastatin (5 mg/kg for 14 days) and doxorubicin (4 x I mg/kg) as compared with either agent acting alone. Lovastatin treatment resulted also in significant reduction of the number of experimental metastasis in doxorubicin-treated mice The results of our studies suggest that lovastatin may enhance the effectiveness of chemotherapeutic agents in the treatment of malignant melanomas.


    128/355

    Tytuł oryginału: Adsorption characteristics of human plasma fibronectin in relationship to cell adhesion.
    Autorzy: Kowalczyńska Hanna M., Nowak-Wyrzykowska Małgorzata, Dobkowski Jacek, Kołos Robert, Kamiński Jarosław, Makowska-Cynka Alicja, Marciniak Ewa
    Źródło: J. Biomed. Mater. Res. 2002: 61 (2) s.260-269, il., tab., bibliogr. 57 poz.
    Sygnatura GBL: 305,818

    Hasła klasyfikacyjne GBL:
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy
  • in vitro

    Streszczenie angielskie: Adsorption of human plasma fibronectin (FN) on nonsulfonated and sulfonated polymer surfaces was studied, by using a polyclonal antiserum to FN and the ELISA method. ELISA signal was recorded as a function of FN concentration in solutions. The concentration dependence of FN binding shows the saturation effect in the range 5-10 ćg/mL. ELISA data are discussed in the terms of a self-assembled monolayer and different conformations of the FN molecule. The early adhesion of L1210 cells to polymer surfaces after prior adsorption of FN on these surfaces was studied under static conditions. In the case of FN adsorbed on sulfonated surfaces, the relative number of adhering cells increased with the increase of the interfacial surface tension (i.e., the cell adhesion depends on the surface density of sulfonic groups). However, in the case of FN adsorbed on nonsulfonated surfaces, the relative number of adhering cells was low and independent on the interfacial surface tension. The ŕ5á1-integrin blocking by a monoclonal antibody resulted on sulfonated polymer surfaces. This indicates that cell adhesion to FN adsorbed on these surfaces is mostly mediated by the ŕ5á1-integrin. In contrast, in the case of FN adsorbed on nonsulfonated surfaces the cell adhesion was not inhibited by the ŕ5á1-integrin blocking.


    129/355

    Tytuł oryginału: Antagonism of picrotoxin-induced changes in dopamine and serotonin metabolism by allopregnanolone and midazolam.
    Autorzy: Maciejak Piotr, Krząścik Paweł, Członkowska Agnieszka I., Szyndler Janusz, Bidziński Andrzej, Walkowiak Jerzy, Kostowski Wojciech, Plaznik Adam
    Źródło: Pharmacol. Biochem. Behav. 2002: 72 (4) s.987-991, il., tab., bibliogr. [11] poz.
    Sygnatura GBL: 312,498

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca doświadczalna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: The effects of allopregnanolone and midazolam, given intracerebroventricularly, on the behavioral and biochemical effects of picrotoxin, were examined in a model of neurotoxin-induced seizures, in mice. After acute injections, midazolam (ED50 = 39.8 nmol) and allopregnanolone (ED50 = 11.0 nmol) produced similar and dose-dependent protection against picrotoxin-induced seizures. Picrotoxin given intraperitoneally at the ED85 dose decreased significantly the concentration of serotonin (5-HT), dopamine (DA), homovanilic acid (HVA) and 3,4-dihydroxyindolacetic acid (DOPAC), in the mouse striatum and the frontal cortex, in the period of time immediately preceding the onset of seizures. A single injection of allopregnanolone more potently, in comparison to midazolam, antagonized the biochemical action of picrotoxin, abolishing its effects on DA, HVA and 5-HT concentration, in the mouse striatum and the frontal cortex. These results for the first time provide a direct argument for an involvement of central dopaminergic and serotonergic systems in the seizure development. The present data add also to the accumulating evidence suggesting a favorable pharmacological profile for some neurosteroids currently considered to have a future role in the management of epilepsy.


    130/355

    Tytuł oryginału: The influence of luteinizing hormone-releasing hormone analog on serum leptin and body composition in women with solitary uterine myoma.
    Autorzy: Nowicki Michał, Adamkiewicz Grażyna, Bryc Władysław, Kokot Franciszek
    Źródło: Am. J. Obstet. Gynecol. 2002: 186 (3) s.340-344, il., tab., bibliogr. 25 poz.
    Sygnatura GBL: 300,265

    Hasła klasyfikacyjne GBL:
  • farmacja
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć żeńska

    Streszczenie angielskie: Objective: The purpose of this study was to examine the influence of luteinizing hormone-releasing hormone analog goserelin on serum leptin and body composition in women with solitary uterine myoma. Study design: Fifteen women who were regularly menstruating and not obese were included. In all subjects, serum concentrations of leptin, insulin, testosterone, progesterone, and estradiol and body mass index and waist-to-hip ratio were measured before and after 4, 8, and 12 weeks of treatment with goserelin (3.6 mg every 4 weeks). Fat mass and lean body mass were measured by dual energy radiographic densitometry at baseline and after 12 weeks of therapy. Data were analyzed by multiple way analysis of variance and both simple and multiple regression. Results: The treatment caused a significant regression of myoma. Body weight, fat, ane lean mass were unchanged. No changes in plasma leptin (even after correction for fat mass) were noted during the treatment. Plasma estradiol decreased below castrate levels. Plasma progesterone decreased significantly, and testosterone tended to decline during the study. At baseline a highly significant positive correlation was found between serum leptin and fat mass. In a multiple regression analysis, neither the change in fat mass nor any of the hormonal parameters explained the significant portion of variance of plasma leptin during the treatment. Conclusion: Pharmacologic gonadectomy does not influence plasma leptin concentrations in women if body fat mass is unchanged.


    131/355

    Tytuł oryginału: Journal of Pharmacy and Pharmacology
    Tytuł polski: Pharmacological properties and SAR of new 1,4-disubstituted piperazine derivatives with hypnotic-sedative activity.
    Autorzy: Chilmonczyk Zdzisław, Mazgajska Maria, Iskra-Jopa Joanna, Chojnacka-Wójcik Ewa, Tatarczyńska Ewa, Kłodzińska Aleksandra, Nowak Jerzy Z.
    Źródło: J. Pharm. Pharmacol. 2002: 54 (5) s.689-698, il., tab., bibliogr. s. 696-698
    Sygnatura GBL: 300,961

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Preparation, pharmacological properties and structure-activity relationships of new pyrimidylpiperazine derivatives, exhibiting sedative and hypnotic activity in mice, are reported. The hypnotic activity of the compounds was comparable with that of zopiclone (the known hypnotic-sedative agent), their interaction with ethanol, however, being much lower. The obtained results suggested that zopiclone and pyrimidylpiperazines 2, 4 and 5 exerted their pharmacological activity through a different mechanism - zopiclone through the interaction with benzodiazepine receptors and compounds 2, 4 and 5 through an unidentified molecular target. The pharmacological properties of compound 3 could be the result of a mixed mechanism of action, combining the properties of zopiclone and those of compounds 2, 4 and 5. A common feature of zopiclone and compounds 2 and 3 was that, after their systemic administration, independently of mechanism of action, together with the hypnotic effect a reduction of the 5-HT turnover in the mouse brain was observed. Minimum structural requirements for the hypnotic activity were formulated. Structural considerations have shown that removing the ŕ-carbonyl group did not influence the drug's ability to inhibit the locomotor activity. However, it did influence its ability to disturb motor coordination or abolish the righting reflex within non-lethal doses.


    132/355

    Tytuł oryginału: Is diminished pubocervical fascia collagen content a risk factor for failure of surgical management of genuine stress urinary incontinence in women?
    Autorzy: Skorupski Paweł, Rechberger Tomasz, Postawski Krzysztof, Woessner jr J. Frederick, Jakowicki Jerzy A.
    Źródło: Eur. J. Obstet. Gynecol. Reprod. Biol. 2002: 102 (2) s.195-198, tab., bibliogr. 19 poz.
    Sygnatura GBL: 305,582

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: Objective: The assessment of relationship between pubocervical collagen content and clinical results of surgical treatment of genuine stress urinary incontinence (GSUI) in women. Methods: Twenty-four women treated for genuine stress urinary inontinence were included into the study. All women underwent the same surgical procedure. The samples of pubocervical fasia were taken at the time of surgery. The contents of acid soluble, pepsin soluble, insoluble fraction of collagen, total collagen and collagen crosslinks were measured. The study of pubocervical fascia collagen metabolism included also estimation of collagen activity. At follow-up done 5 years following surgery, 20 patients reported symptoms of GSUI (study group). Four women were still without symptoms of urine leakage (control group). The biochemical parameters of pubocervical fascia did not show, statistically significant differences between cimpared groups. Conclusions: The pubocervical fascia collagen metabolism does not have impact on the results of anti-incontinence surgery.


    133/355

    Tytuł oryginału: Homozygous combination of calpain 10 gene haplotypes is associated with type 2 diabetes mellitus in a Polish population.
    Autorzy: Malecki Maciej T., Moczulski Dariusz K., Klupa Tomasz, Wanic Krzysztof, Cyganek Katarzyna, Frey Jakub, Sieradzki Jacek
    Źródło: Eur. J. Endocrinol. 2002: 146 (5) s.695-699, tab., bibliogr. 19 poz.
    Sygnatura GBL: 301,344

    Hasła klasyfikacyjne GBL:
  • genetyka
  • endokrynologia

    Typ dokumentu:
  • praca epidemiologiczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: The polymorphisms of two genes have recently been associated with complex forms of type 2 diabetes mellitus (T2DM): calpain 10 and peroxisome proliferator-actvated receptor-ç (PPARç). Calpain 10 is a member of a large family of intracellular protease. It was shown in Mexican-Americans and other populations that variants of three single nucleotide polymorphisms (SNPs), -43, -19, and -63, of this ubiquitously expressed protein influence susceptibility to T2DM. However, substantial differences were shown between ethnic groups in at risk alleles and haplotypes as well as in their attributable risk. Thus, it is important to determine the role of calpain 10 in various populations. Aim: To examine the role of calpain 10 SNPs -43, -19, and -63 in genetic susceptibility to T2DM in a Polish population. Methods: Overall, 377 individuals were examined: 229 T2DM patients and 148 control individuals. The groups were genotyped for calpain 10 SNP-43, SNP-19, and SNP-63. SNP-19 was examined by electrophoresis of the PCR product on agarose gel by size, while the restriction fragment length polymorphism (RFLP) method was used for the two other markers. Difference in allele, genotype, haplotype, and haplotype combination distribution between the groups were examined by Xý test. Results: Distributions of alleles, genotypes, and haplotypes at three loci defined by examined SNPs were not significantly different between the groups. However, the homozygote combination of 121 haplotype was more prevalent in the T2DM group than in the controls (17.9 p.c. vs 10.1 p.c., P = 0.039)...


    134/355

    Tytuł oryginału: Prognostic value of low-dose dobutamine echocardiography in patients with idiopathic dilated cardiomyopathy.
    Autorzy: Drożdż Jarosław, Krzemińska-Pakuła Maria, Plewka Michał, Ciesielczyk Michał, Kasprzak Jarosław D.
    Źródło: Chest 2002: 121 (4) s.1216-1222, il., tab., bibliogr. 30 poz.
    Sygnatura GBL: 300,494

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Study objectives: Dobutamine echocardiography is widely used for the evaluation of myocardial contractile reserve. The purpose of the study was to determine the prognostic value of low-dose dobutamine echocardiography in patients with idiopathic dilated cardiomyopathy (IDCM). Patients: The study group consisted of 77 consecutive patients with recently diagnosed IDCM (mean [ń SD] age, 49 ń 9 years; men, 82 p.c.) and left ventricular (LV) ejection fractions of 40 p.c. Interventions: Two-dimensional and Doppler echocardiographic variables were measured before and after the infusion of dobutamine at the rate of 10 ćg/kg/min for 5 min. Measurements and results: During a mean follow-up period of 63 ń 7 months (range, 49 to 75 months) 30 patients (39 p.c.) died and five patients (6 p.c.) underwent successful heart transplantations. Using multivariate regression analysis, the only significant factors related to fatal outcome or the need for cardiac transpantation were the following: (1) LV end-systolic volume of 150 mL after low-dose dobutamine infusion (odds ratio [OR], 2.2; confidence interval [CI], 1.2 to 4.1; p = 0.011); (2) no decrease of LV end-diastolic volume after dobutamine infusion (OR, 1.9; CI, 1.1 to 3.4; p = 0.031); (3) atrial fibrillation (OR, 2.7; CI, 1.4 to 5.3; p = 0.003); and (4) male gender (OR, 2.6; CI, 1.2 to 5.5; p = 0.017). A scoring system was proposed with one point assigned for each of the above-mentioned factors. The mortality rates for total scores of 0, 1, 2, 3, and 4 were 0 p.c., 19 p.c., 48 p.c., 83 p.c., and 100 p.c., respectively. Conclusion: The response of the LV to low-dose dobutamine infusion adds clinically valuable prognostic information to the evaluation of the patient with IDCM.


    135/355

    Tytuł oryginału: Stimulatory effects of pituitary adenylate cyclase-activating polypeptide on inositol phosphates accumulation in avian cerebral cortex and hypothalamus.
    Autorzy: Nowak Jerzy Z., Pigulowska Aleksandra, Kuba Katarzyna, Zawilska Jolanta B.
    Źródło: Neurosci. Lett. 2002: 323 (3) s.179-182, il., bibliogr. 22 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • ptaki

    Streszczenie angielskie: This study has demonstrated that the short and long form of the pituitary adenylate cyclase-activating polypeptide (PACAP), i.e. PACAP27 and PACAP38, moderately but significantly, and in a concenration (0.5-5 ćM)-dependent manner, stimulated inositol phosphates (IPs) accumulation in myo-[3H]inositol-prelabeled cerebral cortical and hypothalamal slices of chick and duck, and in slices of rat cerebral cortex; both peptides had no effect on IPs formation in rat hypothalamus. Vasoactive intestinal peptide (VIP; 0.5-5 ćM) weakly enhanced IPs accumulation in chick hypothalamus, had no significant action in chick cerebral cortex (in fact there was a tendency to attenuate the IPs response in this tissue), and slighty, but significantly, inhibite the IPs accumulation in rat cerebral cortex. VIP showed no activity in rat hypothalamus. It is cincluded that tha stimulatory action of PACAP on phosphoinositide metabolism in avian cerebral cortex, similar to rat cerebral cortex, is mediated via phospholipase C-linked PAC1 type receptors. In chick hypothalamus, however, there may be a component of VPAC type receptors stimulating IPs formation.


    136/355

    Tytuł oryginału: Long-term therapy with recombinant human erythropoietin decreases percentage of CD152+ lymphocytes in primary glomerulonephritis haemodialysis patients.
    Autorzy: Trzonkowski Piotr, Myśliwska Jolanta, Dębska-Ślizień Alicja, Bryl Ewa, Rachoń Dominik, Myśliwski Andrzej, Rutkowski Bolesław
    Źródło: Nephrol. Dial. Transplant. 2002: 17 (6) s.1070-1080, il., tab., bibliogr. 25 poz.
    Sygnatura GBL: 312,872

    Hasła klasyfikacyjne GBL:
  • immunologia
  • farmacja
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Bacground. Recombinant human erythropoietin (rHuEpo) may affect the human immune system. The aim of the study was to examine changes in CD4+ and CD8+ T-cell subpopulations, the expression of the inhibitory molecule, CD152 on T lymphocytes and the levels of interleukins (IL), 2, 6, 10, 12 and tumour necrosis factor ŕ )TNFŕ) in primary glomerulonephritis chronic haemodialysis (HD) patients before and under rHuEpo treatment. Methods. Expression of T-cell surface molecules was measured in 14 HD patients ex vivo by flow cytometry of lymphocytes sampled from peripheral blood and in viutro using whole blood cell cultures stimulated either with phytohaemagglutinin (PHA) or with physiological as well as non-physiological does or rHuEpo. The concenrations of the cytokines were measured in the supernatans from non-or PHA-stimulated cultures using bioassays (IL2, IL6, TNFŕ) or ELISA test (IL10, IL12). Results. Compared with findings before the start of rHuEpotherapy the CD4+/CD8+ ratio increased after 1 year of follow-up, whereas the percentage of CD152+ peripheral blood lymphocytes decreased. The increase of the CD4+/CD8+ rtio was dependent on a decrease of the percentage of CD8+ cells. The decrease of CD152+ population affected mainly CD8+ CD152+ cells. All these effects became apparent after 6 months of rHuEpo treatment. In vitro stimulation of whole blood cultures revealed that the addition of PHA up-regulated the percentage of CD152+ lymphocytes, while physiological concentrations of rHuEpo decreased the percentage of CD8+ 152+ T cells...


    137/355

    Tytuł oryginału: Carp erythrocyte lipids as a potential target for the toxic action of zinc ions.
    Autorzy: Gabryelak Teresa, Akahori Anna, Przybylska Maria, Jóźwiak Zofia, Brichon Gerard
    Źródło: Toxicol. Lett. 2002: 132 (1) s.57-64, il., tab., bibliogr. s. 63-64
    Sygnatura GBL: 306,158

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta

    Streszczenie angielskie: The effects of zinc on the lipid components of carp erythrocytes (Cyprinus carpio L.) were studied on red blood cells exposed to ZnSO4 (0.01 - 1 mM) for 20 h. The temperatures of the incubation medium were 5 and 20řC for carp collected in winter and summer, respectively. It was observed that increase in the concentration of metal ions in the medium led to significant changes in the content of the main phospholipid classes. Zinc induced the reduction of phosphatidylcholine as well as the increase in the content of phosphatidylserine and phosphatidylinositol. A marked increase in the levels of malondialdehyde and conjugated dienes was also found both in the cells and the incubation medium. Moreover, elevated Zn concentrations caused alterations in the erythrocyte plasma membrane fluidity, estimated by measuring the fluorescence of 1-[4-trimethylaminophenyl]-6-phenyl-1,3,5-hexatriene and 12-[9-anthroyloxy]stearic acid, located on the surface and in the hydrophobic regions of the lipid bilayer. This study in vitro confirmed that higher concentrations of zinc ions induce marked changes in the composition and structure of membrane lipids in carp erythrocytes.


    138/355

    Tytuł oryginału: Tellurium-induced cognitive deficits in rats are related to neuropathological changes in the central nervous system.
    Autorzy: Widy-Tyszkiewicz Ewa, Piechal Agnieszka, Gajkowska Barbara, Śmiałek Mieczysław
    Źródło: Toxicol. Lett. 2002: 131 (3) s.203-214, il., tab., bibliogr. s. 213-214
    Sygnatura GBL: 306,158

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • psychiatria i psychologia
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć męska

    Streszczenie angielskie: The effects of sodium tellurite 0.1 and 0.4 mg/kg were assessed in the Morris water maze. Two days after treatment rats were tested for acquisition (posttreatment days 3-6) and on seventh day on a spatial retention task. Tellurium treatment was found to cause significant impairment in retention of the spatial learning task. Locomotor disturbances were not the cause of the observed effects. Ultrastructural observations showed neuropathological changes in hippocampus subfields and prefrontal cortex with swelling of synapses, astrocytes and astrocytic processes around the vessels in the cerebral cortex neuropil. Severity of the observed changes in glial-neuronal unit was in correlation with the extent of learning impairment. A direct injury of Schwann cells with the secondary myoclasis was noted in the sciatic nerve. Our results indicate that acute treatment with sodium tellurite results in impairment of learning and spatial memory.


    139/355

    Tytuł oryginału: Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production.
    Autorzy: Tada Hiroyuki, Sugawara Shunji, Nemoto Eiji, Takahashi Nobuhiro, Imamura Takahisa, Potempa Jan, Travis James, Shimauchi Hidetoshi, Takada Haruhiko
    Źródło: Infect. Immun. 2002: 70 (6) s.3304-3307, il., tab., bibliogr. 39 poz.
    Sygnatura GBL: 305,564

    Hasła klasyfikacyjne GBL:
  • stomatologia
  • immunologia
  • mikrobiologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.


    140/355

    Tytuł oryginału: Fold-recognition analysis predicts that the Tag protein family shares a common domain with the helix-hairpin-helix DNA glycosylases.
    Autorzy: Bujnicki Janusz M., Rychlewski Leszek
    Źródło: DNA Repair 2002: 1 (5) s.391-395, il., bibliogr. 30 poz.
    Sygnatura GBL: 304,952

    Hasła klasyfikacyjne GBL:
  • genetyka
  • mikrobiologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: The Escherichia coli protein Tag is traditionally regarded as an archetype of one of four classes of N-alkylpurine DNA glycosylases. However, its structure and phylogenetic relationship to other glycosylases emains a mystery. Fold-recognition and sequence profile analyses suggest that Tag shares the catalytic domain with helix-hairpin-helix (HhH) glycosylases such as MutY, AlkA and EndoIII, but its N- and C-termini together form a unique His2Cys2 cluster. The findings presented in this paper provide insight into sequence-structure-function relationships in the Tag family and should aid in a more precise definition of the common core of the HhH superfamily of glycosylases involved in DNA repair.


    141/355

    Tytuł oryginału: International Journal of Cardiology
    Tytuł angielski: Verapamil improves the pacing-induced vasodilatation in symptomatic patients with hypertrophic cardiomyopathy.
    Autorzy: Dimitrow Paweł Petkow, Krzanowski Marek, Grodecki Janusz, Małecka Barbara, Lelakowski Jacek, Kawecka-Jaszcz Kalina, Szczeklik Andrzej, Dubiel Jacek S.
    Źródło: Int. J. Cardiol. 2002: 83 (3) s.239-247, tab., bibliogr. 52 poz.
    Sygnatura GBL: 305,693

    Hasła klasyfikacyjne GBL:
  • farmacja
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The purpose of the study was to assess the effect of varapamil on the response of diastolic coronary flow velocity and coronary vascular resistance to pacing in symptomatic patients with hypertrophic cardiomyopathy. In 14 patients with hypertrophic cardiomyopathy, the coronary flow velocity was detected in the left anterior descending coronary artery using transthoracic Doppler echocardiography. The peak diastolic coronary flow velocity and coronary and coronary vascular resistance was measured at baseline and during pacing. Changes of these parameters induced by the pacing (expressed as the percentage of baseline values) were compared on varapamil treatment and after verapamil withdrawal. The same measurements were obtained in ten control subjects. The results show that, in hypertrophic cardiomyopathy patients, increase in coronary flow velocity during pacing was significantly higher on than off varapamil therapy (64.8 ń 32.5 vs. 41.1 ń 21.3 p.c., P 0.05). In control subjects, pacing-induced increase in coronary flow velocity was comparable to changes in coronary flow velocity in hypertrophic cardiomyopathy patients receiving verapamil (80.2 ń 18.4 vs. 64.8 ń 32.5 p.c., P 0.05). After verapamil withdrawal in hypertrophic cardiomyopathy patients, coronary flow velocity increase during pacing was significantly lower than in control subjects (41.1 ń 21.3 vs. 80.2 ń 18.4 p.c., P 0.05). During pacing the coronary vascular resistance decreased more on verapamil than after drug withdrawal (-34.7 ń 11.7 vs. -24.6 ń 12.9 p.c., P 0.05). In control subjects the coronary...


    142/355

    Tytuł oryginału: Niguldipine impairs the protective activity of carbamazepine and phenobarbital in amygdala-kindled seizures in rats.
    Autorzy: Borowicz Kinga K., Kleinrok Zdzisław, Czuczwar Stanisław J.
    Źródło: Eur. Neuropsychopharmacol. 2002: 12 (3) s.225-233, il., tab., bibliogr. s. 232-233
    Sygnatura GBL: 313,361

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: There is evidence that some calcium (Ca2+) channel inhibitors enhance the protective activity of antiepileptic drugs. Since clinical trials have not provided consistent data on this issue, the objective of this study was to evaluate the interaction of a dihydropyridine, niguldipine, with conventional antiepileptics in amygdala-kindled rats. Niguldipine (at 7.5 but not at 5 mg/kg) displayed a significant anticonvulsant effect, as regards seizure and afterdisharge durations in amygdala-kindled convulsions in rats, a model of complex partial seizures. No protective effect was observed when niguldipine (5 mg/kg) was combined with antiepileptics at subeffective doses, i.e. valproate (75 mg/kg), diphenylhydantoin (40 mg/kg), or clonazepam (0.003 mg/kg). Unexpectedly, the combined treatment of niguldipine (5 mg/kg) with carbamazepine (20 mg/kg) or phenobarbital (20 mg/kg) resulted in a proconvulsive action. BAY k-8644 (an L-type Ca2+ channel activator) did not modify the protective activity of niguldipine (7.5 mg/kg) or the opposite action of this dihydropyridine (5 mg/kg) in combinations with carbamazepine or phenobarbital. A pharmacokinetic interaction is not probable since niguldipine did not affect the free plasma levels of the antiepileptics. These data indicate that the opposite actions of niguldipine alone or combined with carbamazepine (or phenobarbital) were not associated with Ca2+ channel blockade. The present results may argue against the use of niguldipine as an adjuvant antiepileptic or for cardiovascular reasons in patients with complex partial seizures.


    143/355

    Tytuł oryginału: Toxicology Letters
    Tytuł angielski: Peripheral blood lymphocytes and selected serum interleukins in workers operating X-ray equipment.
    Autorzy: Hrycek Antoni, Czernecka-Micińska Aleksandra, Kłuciński Piotr, Badowski Roman
    Źródło: Toxicol. Lett. 2002: 132 (2) s.101-107, tab., bibliogr. s. 106-107
    Sygnatura GBL: 306,158

    Hasła klasyfikacyjne GBL:
  • medycyna pracy
  • radiologia

    Typ dokumentu:
  • praca epidemiologiczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: The objective of these studies was to determine the effect of low X-ray doses on peripheral blood lymphocytes in the workers operating X-ray equipment. In 30 workers and 18 persons who constituted the control group the absolute number of peripheral blood lymphocytes, the number of T CD4+ and T CD8+ lymphocytes, the ratio CD4+/CD8+ and the concentration of serum interleukins (IL-2, IL-4, IL-6) were tested. Subgroups with respect to sex and employment period were selected. A significant increase in IL-2 concentration in workers as compared to controls was observed and significantly decreased IL-4 level in operating X-ray equipment was revealed. The lowest levels of IL-4 were noted in the women subgroup. A high positive, statistically significant correlations between the number of lymphocytes and the concentration of IL-2 and the level of IL-4 were observed. Moreover, our studies revealed that lymphocyte number and interleukin concentrations in serum of X-ray operators do not depend on the assumed ranges of the length of employment period.


    144/355

    Tytuł oryginału: Melanin potentiates gentamicin-induced inhibition of collagen biosynthesis in human skin fibroblasts.
    Autorzy: Wrześniok Dorota, Buszman Ewa, Karna Ewa, Nawrat Piotr, Pałka Jerzy
    Źródło: Eur. J. Pharmacol. 2002: 446 (1/3) s.7-13, il., bibliogr. s. 12-13
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja
  • toksykologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: One of the recognized side effects of gentamicin is ototoxicity. The mechanism underlying the organ specificity of this side effect of gentamicin has not been fully established. In view of the fact that a number of pharmacologic agents are known to from complexes with melanin and melanin is an abundant constituent of the inner ear tissues, we determined whether gentamicin interacts with melanin and how this process affects the biosynthesis of collagen in cultured human skin fibroblasts. Our results indicate that gentamicin forms comples with melanin. The amount of gentamicin bound to melanin increases with increasing of initial drug concentration. The Scatchard plot analysis of drug binding to melanin showed that at least two classes of independent binding sites are implicated in gentamicin-melanin complex formation: one class with an association constant K1 ~ 4 x 10**3 M**-1, and the second class with an association constant K2 ~ 3 x 10**2 M**-1. The number of total binding sites (n1 + n2) was calculated as about 1.36 ćmol gentamicin per 1 mg melanin. We have suggested that prolidase, an enzyme involved in collagen metabolism, may be one of the targets for gentamicin-induced inhibition of collagen biosynthesis. We found that gentamycin-induced inhibition of prolidase activity (IC50 ~ 100 ćM) and collagen biosynthesis (IC50 ~ 100 ćM). At this concentration of gentamicin, DNA biosynthesis in human skin fibroblasts was inhibited only by about 30 p.c. Melanin at 100 ćg/ml produced about 25 p.c. inhibition of DNA synthesis and about 30 p.c. inhibition of prolidase activity, ...


    145/355

    Tytuł oryginału: Synthesis and effects on chloroquine susceptibility in Plasmodium falciparum of a series of new dihydroanthracene derivatives.
    Autorzy: Alibert Sandrine, Santelli-Rouvier Christiane, Pradines Bruno, Houdoin Carole, Parzy Daniel, Karolak-Wojciechowska Janina, Barbe Jacques
    Źródło: J. Med. Chem. 2002: 45 (15) s.3195-3209, il.,tab., bibliogr. 47 poz.
    Sygnatura GBL: 312,133

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca doświadczalna

    Streszczenie angielskie: To suggest a mechanism of action for drugs capable to reverse the chloroquine resistance, a new set of 9,10-dihydro-9,10-ethano and ethenoanthracene derivatives was synthesized and compounds were tested with the aim to assess their effect on chloroquine susceptibility in Plasmodium falciparum resistant strains. With respect to this, reversal of resistance and change in drug accumulation were compared. Structure - activity relationship and molecular modeling studies made it possible to define a pharmacophoric moiety for reversal agents and to propose a putative model of interaction with some selected amino acids.


    146/355

    Tytuł oryginału: Ethanol-induced neurotoxicity is counterbalanced by increased cell proliferation in mouse dentate gyrus.
    Autorzy: Pawlak Robert, Skrzypiec Anna, Sulkowski Stanisław, Buczko Włodzimierz
    Źródło: Neurosci. Lett. 2002: 327 (2) s.83-86, il., bibliogr. 19 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • neurologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca doświadczalna

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: Chronic ethanol abuse leads to degenerative changes in the hippocampus, which may result in subsequent cognitive impairment. Since the hippocampus retains the ability to produce neurons throught adulthood, in the present study we examined if ethanol-induced neuronal loss could be counterbalanced by cell proliferation in mouse dentate gyrus (DG). A total of 14 days of ethanol administration resulted in marked increase in cells positive for TdT-mediated dUTP nick-end labeling in all hippocampal regions studied, indicating that neurons die throughout the hippocampus by apoptotic mechanism. However, cresyl violet staining revealed approximately 20 p.c. neuronal loss following ethanol administration in CA1 and CA2 fields (P 0.01 and P 0.05, respectively), but not in DG. At the same time ethanol caused 2-fold increase in the number of proliferating cells in subgranular zone of DG. Thus, long-term ethanol intoxication causes permanent damage to CA1 and CA2, but not to DG which can be counterbalanced by ongoing neurogenesis.


    147/355

    Tytuł oryginału: Immune reconstitution after haematopoietic cell transplantation in children: immunophenotype analysis with regard to factors affecting the speed of recovery.
    Autorzy: Kalwak Krzysztof, Gorczyńska Ewa, Toporski Jacek, Turkiewicz Dominik, Słociak Małgorzata, Ussowicz Marek, Latos-Grażyńska Elżbieta, Król Marzena, Bogusławska-Jaworska Janina, Chybicka Alicja
    Źródło: Br. J. Haematol. 2002: 118 (1) s.74-89, il., tab., bibliogr. s. 87-89
    Sygnatura GBL: 303,964

    Hasła klasyfikacyjne GBL:
  • pediatria
  • immunologia
  • transplantologia
  • hematologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Immune reconstitution was studied prospectively in 66 children who underwent 77 haematopoietic cell transplantations (HCT): 46 autologous HCTs in 39 patients and 31 allogeneic HCTs in 27 patients. We studied the dynamic analysis of immune recovery with regard to potential factors affecting its speed, including age, type of HCT, diagnosis, graft-versus-host disease (GvHD) and cytomegalovirus (CMV) infection reactivation. Absolute counts of different lymphocyte subsets and immunoglobulin serum levels were determined in peripheral blood of patients on d -7 and +16, and then at various intervals up to 24 months post transplant. Common patterns of immune recovery after both allogeneic and autologous HCT were identified: (I) CD4+CD45RO+ peripheral T-cell expansion on d +16; (II) inverted CD4+:CD8+ ratio from D+30 onwards; (III) rapid natural killer (NK) cell (CD16 ń CD56+) count normalization. We observed prolonged T-cell lymphopenia (CD3+, CD3+CD4+, CD4+CD45RA+) until 24 months after autologous HCT, whereas in the allogeneic setting CD3+CD4+ cells, including naive CD45RA+ cells, returned to normal values at 9 months post transplant. Age 10 years and coexistence of GvHD and CMV reactivation were associated with a substantial delay in T- (CD4+, including CD45RA+) and B-cell recovery after allogeneic HCT. Multidrug GvHD prophylaxis resulted in impaired T-(CD4+, CD4+CD45RA+) and B-cell reconstitution only in the early phase after allogeneic HCT (up to 4 months). Our results demonstrated that T-cell recovery was severely impaired in children after autologous HCT...


    148/355

    Tytuł oryginału: Correlation of blood lymphocyte CTLA-4 (CD152) induction in Hodgkin's disease with proliferative activity, interleukin 2 and interferon-gamma production.
    Autorzy: Kosmaczewksa Agata, Frydecka Irena, Boćko Dorota, Ciszak Lidia, Teodorowska Renata
    Źródło: Br. J. Haematol. 2002: 118 (1) s.202-209, il., tab., bibliogr. s. 207-209
    Sygnatura GBL: 303,964

    Hasła klasyfikacyjne GBL:
  • immunologia
  • hematologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Expression of the downregulatory CTLA-4 molecule was determined on unstimulated and anti-CD3+ recombinant interleukin 2 (rIL-2)-stimulated peripheral blood T cells in Hodgkin's disease (HD) and correlated with the T-cells' proliferative activity, IL-2 and interferon (IFN)-gamma production. There was a negligible percentage of CTLA-4+/CD3+ cells before culture. The mean percentage of CTLA-4+/CD3+ lymphocytes increased gradually, peaked after 72 h of stimulation and returned to basal values after 96 h of stimulation. The mean proportion of CTLA-4+/CD3+ cells from untreated patietns was significantly higer after 24, 48 and 72 h of stimulation comppared with controls. The mean percentage of CTLA-4+/CD3+ cells from patietns in clinical remission (CR) was lower than that of untreated patients, but remained significantly higher compared with controls. Lymphocytes from untreated HD patients showed impaired proliferative activity, IL-2 and IFN-gamma production compared with controls. The proliferative activity of the lymphocytes, IL-2 and IFN-gamma production remained significantly lower in CR compared with controls. The proportion of CTLA-4+/CD3+ cells negatively correlated with proliferative activity, IL-2 and IFN-gamma production in HD patients and controls. However, some untreated patients as well as patients in CR with normal mean fluorescence intensity values of CTLA-4 showed unimpaired T-cell function tests. Our study provides the first evidence of an increased expression of downregulatory CTLA-4 molecule on stimulated T-cells in HD, which could be one of the mechanisms of immune deficiency in this disease.


    149/355

    Tytuł oryginału: Photoreactivity of aged human RPE melanosomes: a comparison with lipofuscin.
    Autorzy: Różanowska Małgorzata, Korytowski Witold, Różanowski Bartosz, Skumatz Christine, Boulton Mike E., Burke Janice M., Sarna Tadeusz
    Źródło: Invest. Ophthalmol. Vis. Sci. 2002: 43 (7) s.2088-2096, il., bibliogr. 57 poz.
    Sygnatura GBL: 311,976

    Hasła klasyfikacyjne GBL:
  • okulistyka

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Purpose. To determine whether aging is accompanied by changes in aerobic photoreactivity of retinal pigment epithelial (RPE) melanosomes isolated from human donors of different ages, and to compare the photoreactivity of aged melanosomes with that of RPE lipofuscin. Methods. Human RPE pigment granules were isolated from RPE cells pooled into groups according to the age of the donors. Photoreactivity was determined by blue-light-induced oxygen uptake and photogeneration of reactive oxygen species. Short-lived radical intermediates were detected by spintrapping, hydrogen peroxide by an oxidase electrode, singlet oxygen by cholesterol assay, and lipid hydroperoxides by iodometric assay. Results. Blue-light photoexcitation of melanosomes resulted in age-related increases in both oxygen uptake and the accumulation of superoxide anion spin adducts. The efficiencies of these processes, however, were still significantly lower than that induced by photoexcited lipofuscin. During irradiation of melanosomes, a substantial amount of oxygen was converted into hydrogen peroxide, whereas for lipofuscin, hydrogen peroxide accounted for not more than 3 p.c. of oxygen consumed. In contrast to lipofuscin, photoexcited melanosomes did not substantially increase the rate of oxidative reactions in the presence of polyunsaturated lipids of albumin. However, oxygen uptake was significantly elevated in the presence of ascorbate. Thus, the rate of photo-induced oxygen uptake in samples containing both ascorbate and melanosomes approached that observed in lipofuscin samples...


    150/355

    Tytuł oryginału: Human papillomavirus and oncogenesis: critical evaluation of recent findings.
    Autorzy: Majewski Sławomir, Jabłonska Stefania
    Źródło: Int. J. Dermatol. 2002: 41 (6) s.319-320, bibliogr. 16 poz.
    Sygnatura GBL: 305,645

    Hasła klasyfikacyjne GBL:
  • onkologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie


    151/355

    Tytuł oryginału: Association of asthma and total IgE levels with human leucocyte antigen-DR in patients with grass allergy.
    Autorzy: Woszczek G[rzeogrz], Kowalski M[arek] L., Borowiec M[aciej]
    Źródło: Eur. Respir. J. 2002: 20 (1) s.79-85, il., tab., bibliogr. 28 poz.
    Sygnatura GBL: 312,824

    Hasła klasyfikacyjne GBL:
  • immunologia
  • pulmonologia
  • otorynolaryngologia

    Typ dokumentu:
  • tytuł obcojęzyczny
  • praca opublikowana za granicą
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Exposure to grass pollens during the pollen season, reveals in sensitive patients symptoms of allergic rhinitis, conjunctivitis and/or bronchial asthma. It is not well understood why, in some patients, only symptoms of rhinits occur while in others similar exposure causes symptoms of asthma and rhinitis. An association study is reported here, where the possible contribution of human leucocyte antigen (HLA)-DR gene polymorphisms to differential phenotypic expression of symptoms in patients with grass-pollen allergy was determined. HLA-DR genotyping was performed by the polymerase chain reaction with the sequence-specific primers method in 82 patients with symptoms of seasonal allergic rhinitis and/or bronchial asthma and 52 healthy nonatopic control subjects. A significant association was found between HLA-DRB1*02, B5* haploytpe and asthma phenotype in patients with grass-pollen allergy when compared to patients with rhinitis only. Significantly higher total serum immunoglobulin E levels were observed in patients with HLA-DRB1*01 alleles in comparison to patients without these alleles. The data in this study suggest that human leucocyte antigen-DR locus, or other genes in linkage disequilibrium, may play an important role in asthma phenotype expression in patients with grass-pollen allergy as well as in determining total immunoglobulin E levels in these patients.


    152/355

    Tytuł oryginału: Functional and clinical characterization of KCNJ2 mutations associated with LQT7 (Andersen syndrome).
    Autorzy: Tristani-Firouzi Martin, Jensen Judy L., Donaldson Matthew R., Sansone Valeria, Meola Giovanni, Hahn Angelika, Bendahhou Said, Kwieciński Hubert, Fidziańska Anna, Plaster Nikki, Fu Ying-Hui, Ptacek Louis J., Tawil Rabi
    Źródło: J. Clin. Invest. 2002: 110 (3) s.381-388, il., tab., bibliogr. 38 poz.
    Sygnatura GBL: 310,487

    Hasła klasyfikacyjne GBL:
  • pediatria
  • genetyka
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • noworodki
  • niemowlęta
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.

    Streszczenie angielskie: Andersen syndrome (AS) is a rare, inherited disorder characterized by periodic paralysis, long QT (LQT) with ventricular arrhythmias, and skeletal developmental abnormalitise. We recently established that AS is caused by mutations in KCNJ2, which encodes the inward rectifier K+ channel Kir2.1. In this report, we characterized the functional consequences of three novel and seven previously described KCNJ2 mutations using a two-microelectrode voltage-clamp technique and correlated the findings with the clinical phenotype. All mutations resulted in loss of function and dominant-negative suppression of Kir2.1 channel function. In mutation carriers, the frequency of periodick paralysis was 64 p.c. and dysmorphic features 78 p.c. LQT was the primary cardiac manifestation, present in 71 p.c. of KCNJ2 mutation carriers, with ventricular arrhythmias present in 64 p.c. While arrhythmias were common, none of our subjects suffered sudden cardiac death. To gain insight into the mechanismof arrhythmia susceptibility, we simulated the effect of reducdd Kir2.1 using a ventricular myocyte model. A reduction in Kir2.1 prolonged the termkinal phase of the cardiac action potential, and n the setting of reduced extracellular K+, nduced Na+/Ca2+ exchanger-dependent delayed afterdepolarizations and spontaneous arrhythmias. These findings suggest that the substrate for arrhythmia susceptibility in AS is distinct from the other forms of inherited LQT syndrome.


    153/355

    Tytuł oryginału: 1- methyl-4-phenylpyridinium and 3-nitropropionic acid diminish cortical synthesis of kynurenic acid via interference with kynurenine aminotransferases in rats.
    Autorzy: Luchowski Piotr, Luchowska Elżbieta, Turski Waldemar A., Urbańska Ewa M.
    Źródło: Neurosci. Lett. 2002: 330 (1) s.49-52, il., bibliogr. 22 poz.
    Sygnatura GBL: 305,936

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The aim of the present study was to evaluate the effect of mitochondrial inhibitors, 1-methyl-4-phenylpyridinium (MPP+) and 3-nitropropionic acid (3-NPA), on the brain production of endogenous glutamate antagonist, kynurenic acid (KYNA). MPP+ and 3-NPA dose-dependently impaired the synthesis of KYNA in rat cortical slices. Enzymatic studies revealed the MPP+ inhibits in a concentration-dependent manner the activity of kynurenine aminotransferase II (KAT II), but not the activity of kynurenine aminotransferase I (KAT I). 3-NPA impaired the activity of both enzymes, KAT I and KAT II. Thus, MPP+ - and 3-NPA-evoked neurotoxicity may be at least partially associated with the depletion of KYNA.


    154/355

    Tytuł oryginału: Cardiac arrest related to coronary spasm in patients with variant angina: a three-case study.
    Autorzy: Seniuk W., Mularek-Kubzdela T[atiana], Grygier M[arek], Grajek S., Cieśliński A[ndrzej]
    Źródło: J. Intern. Med. 2002: 252 (4) s.368-376, il., bibliogr. 33 poz.
    Sygnatura GBL: 300,763

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kazuistyczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We present three patients with variant angina pectoris and episodes of cardiac arrest. All of them had typical clinical symptoms, ST-segment changes in electrocardiogram, and coronary artery spasm confirmed by arteriography. They were treated with high doses of calcium antagonists and nitrates. An automatic cardioverter-defibrillator was implanted in the patient who developed ventricular fibrillation despite therapy with calcium antagonists. In another patient a DDD pacemaker was implanted becasue of high-degree atrioventricular blok.


    155/355

    Tytuł oryginału: Porihvnjalnijj analihz sistem opodatkuvannja zarobihtnoji płati pracihvnikihv aptek v Ukrajinih hi Polshhih.
    Tytuł angielski: The comparative analysis of systems of the taxation of wages of pharmacies workers i Ukraine and Poland.
    Autorzy: Tereshhuk S. Ih., Moska Dionizy, Moska Dariusz, Tereshhuk T. O., Labus O.
    Źródło: Farm. Zh. 2002 (3) s.27-32, il., tab., bibliogr. 9 poz., sum., rez.
    Sygnatura GBL: 304,380

    Hasła klasyfikacyjne GBL:
  • ekonomika
  • farmacja

    Typ dokumentu:
  • praca epidemiologiczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The compareative analysis of taxacion systems of private individuals and ownershipers's wages in Ukraine and Poland has been carried out. It is established, that more flexible and transparent system of the taxes is used in Poland, according to which the base for the taxation decreases on living expenses of the citizens.


    156/355

    Tytuł oryginału: Group II mGlu receptor agonists inhibit behavioural and electrophysiological effects of DOI in mice.
    Autorzy: Kłodzińska Aleksandra, Bijak Maria, Tokarski Krzysztof, Pilc Andrzej
    Źródło: Pharmacol. Biochem. Behav. 2002: 73 (2) s.327-332, il., bibliogr. s. 331-332
    Sygnatura GBL: 312,498

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: It has been suggested that metabotropic glutamate (mGlu) receptor agonists selective for Group II mGlu receptors may have antipsychotic action. Therefore, we studied whether the effects, which could be related to psychotomimetic action of hallucinogenic drugs, are inhibited by Group II mGlu receptor agonists. The selective mGlu2/3 agonist LY354740 and LY379268 inhibited (ń)1-(2,5-dimethoxy-4-iodophenyl)-2-aminoproprane (DOI)-induced head twitches in mice in a dose-dependent manner. Furthermore, LY379268 suppressed an increase in the frequency of sponataneous excitatory synaptic potentials induced by bath-applied DOI in layer V pyramidal cells recorded in the murine medial frontal cortex. The data indicate that Group II mGlu receptor agonist may counteract the effects of hallucinogenic drugs.


    157/355

    Tytuł oryginału: Sex hormone-binding globulin polymorphisms in familial and sporadic breast cancer.
    Autorzy: F”rsti Asta, Jin Quinren, Grzybowska Ewa, S”derberg Magnus, Zientek Helena, Siemińska Marzena, Rozgozińska-Szczepka Jadwiga, Chmielik Ewa, Utracka-Hutka Beata, Hemminki Kari
    Źródło: Carcinogenesis 2002: 23 (8) s.1315-1320, il., tab., bibliogr. 37 poz.
    Sygnatura GBL: 312,779

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: Ovarian steroids are one of the strongest risk factors for breast cancer. Sex hormone-binding globulin (SHBG) binds and transports sex steroids in the blood, regulating their bioavailable fraction and access to target cells. It can also inhibit the estradiol-induced proliferation of breast cancer cells through its membrane receptor. Three coding-region polymorphisms, which oead to an amino acid change, have been reported. We studied the influence of these three polymorphisms on breast cancer risk in three different populations: polish familial breast cancer cases, 27 p.c. of them carrying a BRCA1/BRCA2 mutation, Nordic familial and sporadic breast cancer cases. The reported G to A polymorphism in exon 1 was not found in the 423 analyzed samples. Instead, we found a C to T transition causing an arg to cys amino acid change within the same codon in one polish breast cancer patient and her daughter. Both of them were heterozygotes for the exon 8 to A polymorphism as well. They were diagnosed for bilateral breast cancer and carried a BRCA1 mutation (5382insC). Analysis of the tumor samples showed that they had lost the wild-type allele both at exons 1 and 8 of the SHBG gene. Analysis of the other Polish samples showed no correlation of the exon 8 polymorphism to breast cancer, bilateral breast cancer, BRCA1/BRCA2 mutations or age at diagnosis. No association of the exon 8 polymorphism with breast cancer in the Nordic familial or sporadic cases was found. The C to T polymorphism located in exon 4 was rare in all the studied populations (overal allele frequency 0.011)...


    158/355

    Tytuł oryginału: Meta- and pooled analyses of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk.
    Autorzy: Benhamou Simone, Lee Won Jin, Alexandrie Anna-Karin, Boffetta Paolo, Bouchardy Christine, Butkiewicz Dorota, Brockm”ller Jurgen, Clapper Margie L., Daly Ann, Dolzan Vita, Ford Jean, Gaspari Laura, Haugen Aage, Hirvonen Ari, Husgafvel-Pursiainen Kirsti, Ingelman-Sundberg Magnus, Kalina Ivan, Kihara Masahiro, Kremers Pierre, Le Marchand Loic, London Stephanie J., Nazar-Stewart Valle, Onon-Kihara Masako, Rannug Agneta, Romkes Marjorie, Ryberg David, Seidegard Janeric, Shields Peter, Strange Richard C., Stcker Isabelle, To-Figueras Jordi, Brennan Paul, Taioli Emanuela
    Źródło: Carcinogenesis 2002: 23 (8) s.1343-1350, il., tab., bibliogr. 54 poz.
    Sygnatura GBL: 312,779

    Hasła klasyfikacyjne GBL:
  • pulmonologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Susceptibility to lung cancer may in part be attributable to inter-individual variability in metabolic activation or detopxification of tobacco carcinogens. The glutathione S-transferase M1 (GSTM1) genetic polymorphism has been extensively studied in this context; two recent meta-analyses of case-control studies suggested an association between GSTM1 deletion and lung cancer. At least 15 studies have been published after these overviews. We undertook a new meta-analysis to summarize the results of 43 published case-control studies including 18000 individuals. A slight excess of risk of lung cancer for individuals with the GSTM1 null genotype was found (odds ratio (OR) = 1.17, 95 p.c. confidence interval (CI) 1.07 - 1.27). No evidence of publication bias was found (P = 0.4), however, it is not easy to estimate the extent of such bias and we cannot rule out some degree of publications bias in our rsults. A pooled analysis of the original dsata of about 9500 subjects involved in 21 case-control studies from the international Collaborative Study on genetic Dusceptibility to Environmental Carcinogens (GSEC) data set was performed to assess the role of gsTM1 genotype as a modifier of the effect of smoking on lung cancer risk with adequate power. Analyses revealed no evidence of increased risk of lung cancer among carriers of the GSTM1 null genotype (age-, gender- and center-adjusted OR = 1.08, 95 p.c. CI 0.98 - 1.18) and no evidence of interaction between GSTM1 genotype and either smoking status or cumulative tobacco consumption.


    159/355

    Tytuł oryginału: Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells.
    Autorzy: Baj-Krzyworzeka Monika, Majka Marcin, Pratico Domenico, Ratajczak Janina, Vilaire Gaston, Kijowski Jacek, Reca Ryan, Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
    Źródło: Exp. Hematol. 2002: 30 (5) s.450-459, il., tab., bibliogr. 39 poz.
    Sygnatura GBL: 305,749

    Hasła klasyfikacyjne GBL:
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: objective. peripheral blood plateled-derived microparticles (PMPs) circulate in blood and may interact directly with target cells affecting their various biological functions. methods. To investigate the effect of human PMPs on hematopoiesis, we first phenotyped them for expression of various surface molecules and subsequently studied various biological responses of normal stem/progenitor (CD34+) and more differentiated precursor cells as well as several leukemic cell lines to PMPs. Results. We found that, in addition to platelet-endothelium attachment receptors (CD41, CD61 and CD62), PMPs express G-protein-coupled seven transmembrane-span receptors such as CXCR4 and PAR-1; cytokine receptors including TNF-RI, TNF-RII, and CD95; and ligands such as CD40L and PF-4. Moreover, we found that several of these receptors could be transferred by PMPs to the membranes of normal as well as malignant cells and observed that PMPs: 1) chemoattract these cells, 2) increase their adhesion, proliferation, and survival, and 3) activate in these cells various intracellular signaling cascades including MAPK p42/44, PI-3K-AKT, and STAT proteins. The biological effects of PMPs were only partly reduced by heat inactivation or trypsin digest, indicating that, in addition to the protein components of PMPs, lipid components are also responsible for their biological activity. conclusion. We conclude that PMPs modulate biological functions of hematopoietic cells and postulate that they play an important but as yet not fully understood role in intracellular cross-talk in hematopoiesis...


    160/355

    Tytuł oryginału: The effect of verapamil on the antioxidant defence system in diabetic kidney.
    Autorzy: Kędziora-Kornatowska K., Szram S., Kornatowski T., Szadujkis-Szadurski L., Kędziora J., Bartosz G.
    Źródło: Clin. Chim. Acta 2002: 322 (1/2) s.105-112, tab., bibliogr. 44 poz.
    Sygnatura GBL: 303,881

    Hasła klasyfikacyjne GBL:
  • farmacja
  • nefrologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Bacground: The aim of the study was to examine the effect of verapamil (VP) on lipid peroxidation and activities of key antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px); as well as on glomerular basement membrane (GBM) thickness in streptozotocin-induced diabetic kidney in rats. Methods: Wistar male rats were divided into three groups, 12 rats each: the control (C), diabetic rats (DR), and DR receiving VP, 7 mg/kg body weight in drinking water (DR + VP). Blood glucose (BG) and HbA1c levels, 24-h urinary albumin excretion (UAE) and body weight (BW) were measured every week (0-12 weeks). After 6 and 12 weeks, the animals were sacrificed and malondialdehyde (MDA) content activatives of SOD, CAT and GSH-Px were determined in the kidney homogenate supernatans. Electron micrographs of the glomeruli were scanned and porhphometric investigations were performed by means of a computer image analysis system to compare the glomerular basement basal membrane (GBM) thickness. results: The levels of BG, HbA1c and UAE in DR were significantly higher than in the C group. A progressive increase in the MDA level and a decrease in the SOD, CAT and GSH-Px activaties in the kidney of DR were observed after 6 and 12 weeks. VP administration did not affect BW changes, BG and HbA1 levels in DR. VP decreased lipid peroxidation and augmented the activities of antioxidant enzymes studied in the kidneys of DR as well as decreased kidney weight, GBM thickness and albuminuria in DR. Conclusions: These results confirm the role of oxidative stress in the development of diabetic nephropathy and point to the possible antioxidative mechanism of the nephroprotective action of VP.


    161/355

    Tytuł oryginału: Is the predictive power of previous fractures for new spine and non-spine fractures associated with biochemical evidence of altered bone remodelling? The EPOS study.
    Autorzy: Vergnaud P., Lunt M., Scheidt-Nave C., Poor G., Gennari C., Hoszowski K., Lopez Vaz A., Reid D. M., Benevolenskaya L., Grazio S., Weber K., Miazgowski T., Stepan J. J., Masaryk P., Galan F., Bruges Armas J., Lorenc R., Havelka S., Perez Cano R., Seibel M., Armbrecht G., Kaptoge S., O'Neill T. W., Silman A. J., Felsenberg D., Reeve J., Delmas P. D.
    Źródło: Clin. Chim. Acta 2002: 322 (1/2) s.121-132, tab., bibliogr. 49 poz.
    Sygnatura GBL: 303,881

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Bacground: In the European Prospective Osteoporosis Study (EPOS), a past spine fracture increased risk of an incident fracture 3.6 - 12-fold even after adjusting for BMD. We examined the possibility that biochemical marker levels were associated with this unexplained BMD-independent element of fracture risk. Each of 182 cases in EPOS of spine or non-spine fracture that occurred in 3.8 years of follow-up was matched by age, sex and study centre with two randomly assigned never-fractured controls and one case of past fracture. Analysis measured blind were: osteocalcin, bone-specific alkaline phosphatase, total alkaline phosphatase, serum creatinine, calcium, phosphatase and albumin, together with the collagen cross-links degradation products serum CTS and urine CTX. Most subjects also had bone density neasured by DXA. Results: Cases who had recent fractures did not differ in marlker levels from cases who had their had last fracture more than 3 years previously. No statistically significant effect of recent fracture was found for any marker except osteocalcin, which was 17.6 p.c. lower in recent peripheral cases compared to unfractured controls (p 0.05) and this was independent of BMD. Conclusion: Past fracture as a risk indicator for future fracture is not strongly mediated through increased bone turnover.


    162/355

    Tytuł oryginału: Persistent oxidative stress in colorectal carcinoma patients.
    Autorzy: Gackowski Daniel, Banaszkiewicz Zbigniew, Rozalski Rafał, Jawień Arkadiusz, Oliński Ryszard
    Źródło: Int. J. Cancer 2002: 101 (4) s.395-397, tab., bibliogr. 34 poz.
    Sygnatura GBL: 305,029

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: We examine whether the level of 8-oxo-2'-deoxyguanosine (8-oxodGuo) in lymphocytes DNA is higher in colon cancer when compared to the control group. Factors that may influence oxidative stress such as antioxidant vitamins and uric acid were also determined. Blood samples were obtained from a control group of 55 healthy persons and 43 colon cancers. 8-OxodGuo level and the vitamins concentraction were measured by high-performance liquid chromatography. The levels of 8-oxodGuo were significantly higher whereas the concentractions of the vitamins and uric acid were significantly lower in colon cancer patients than in control group. Therefore, the decreased concentration of antioxidant vitamins together with lower amount of uric acid may be responsible for the formation of pro-oxidative environment in blood of colorectal carcinoma patients.


    163/355

    Tytuł oryginału: Effects of pentylenetetrazol-induced kindling of seizures on rat emotional behavior and brain monoaminergic systems.
    Autorzy: Szyndler Janusz, Rok Paulina, Maciejak Piotr, Walkowiak Jerzy, Członkowska Agnieszka I., Sienkiewicz-Jarosz Halina, Wisłowska Aleksandra, Zienowicz Małgorzata, Lehner Małgorzata, Bidziński Andrzej, Kostowski Wojciech, Płaźnik Adam
    Źródło: Pharmacol. Biochem. Behav. 2002: 73 (4) s.851-861, il., tab., bibliogr. [23] poz.
    Sygnatura GBL: 312,498

    Hasła klasyfikacyjne GBL:
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The influence of pentylenetetrazol (PTZ)-induced kindling of seizures on the rat emotional behaviour, the brain monoamine turnover rate measured in vitro, and correlation between behavioral and biochemical parameters, were examined in rats. The repeated administration of PTZ (35 mg/kg, ip) evoked kindled seizures in rats (Stage 4 or 5 of clonic - tonic convulsions - maximum). PTZ kindling caused selective changes in the rat emotional behavior, present in some models of anxiety only (a decreased freezing time in the conditioned freezing test and a decreased spontaneous and aversively conditioned ultrasonic vocalization). Simultaneously, PTZ kindling decreased the concentration of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the prefrontal cortex, decreased the DA (HVA/DA ratio) turnover rate in the striatum, and inhibited the serotonin (5-HT) metabolism (5-HIAA/5-HT ratio) in the hippocampus and the prefrontal cortex. Correlations between dopamine (DA) or 5-HT regional metabolic rats in brain structures and animal behavior were either abolished or reversed in PTZ-kindled animals. It is concluded that both DA and 5-HT systems contribute to the emotional effects of PTZ-induced kindling of sizures. The hypothesis is put forward that PTZ kindling-induced inhibition of the serotonergic innervation may lead to the compensatory increase in 5-HT1A receptors in the dentate gyrus of the hippocampus, thus evoking the anxiolytic-like changes in animal behavior.


    164/355

    Tytuł oryginału: Temperature-sensitive ovarian carcinoma cell line (OvBH-1).
    Autorzy: Bar Julia K., Harłozińska Antonina, Kartarius Sabine, Montenarh Mathias, Wyrodek Ewa, Parkitna Rodriquez Jan M., Kochman Marian, Ożyhar Andrzej
    Źródło: Jpn. J. Cancer Res. 2002: 93 (9) s.976-985, il., bibliogr. 65 poz.
    Sygnatura GBL: 301,570

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca kazuistyczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: OvBH-1 cells from a patient with ovarian clear cell carcinoma were established and their biochemical status was analyzed. Cells grown at 37řC exhibited normal cell cycle distribution, whereas the cells shifted to 31řC were arrested in the G2/M phase of the cell cycle. Immunochemical analysis using anti-p53 antibodies (DO-1, PAb240, PAb421, and PAb1620) revealed that only the DO-1 antibody reacted with p53 with a high and similar percentage at both temperatures. PAb240 reacted with a low percentage of cells at 37řC and no reaction was observed at 31řC. PAb421 antibody stained a significantly lower percentage of cells at 37řC than at 31řC. Cells were not stained with PAb1620 antibody and were negative for antibodies against p21**WAF1 and MDM2 proteins independently of the temperature. Sequencing of all coding exons of the p53 gene demonstrated only a neutral genetic polymorphism, i.e. a G-to-A substitution (GAG to GAA) at nucleotide position 13 432. Thus, the observed temperature sensitivity of OvBH-1 cells cannot be ascribed to a p53 primary structure mutation. Based upon immunochemical analyses, we consider, however, that p53 in nuclei of OvBH-1 cells is in a highly unstable conformation. Furthermore, the N-terminal portion of the p53 protein at Ser20 has not been modified, and Lys373 and/or Ser 378 of the C-terminus is acetylated and/or phosphorylated. The nuclear location signal of p53 is preserved. Induction of MDM2 protein is uncoupled from the cell regulatory machinery and the induction of p21**WAF1 by p53 is impaired in OvBH-1 cells.


    165/355

    Tytuł oryginału: Effect of chronic imipramine or electroconvulsive shock on the expression of mGluR1a and mGluR5a immunoreactivity in rat brain hippocampus.
    Autorzy: Śmiałowska M., Szewczyk B., Brański P., Wierońska J. M., Pałucha A., Bajkowska M., Pilc A.
    Źródło: Neuropharmacology 2002: 42 (8) s.1016-1023, il., bibliogr. s. 1022-1023
    Sygnatura GBL: 305,141

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Previous studied showed that chronic electroconvulsive shock (ECS) or impiramine treatment induced a subsensitivity of group I metabotropic glutamate receptors (mGluR) in hippocampus. In the present study effects of antidepressant treatment on the expression og mGluR1a and mGluR5a, belonging to the group I mGluR, were investigated in rat brain hippocampus using immunohistochemical and Western blot methods, respectively. Male Wistar were treated singly or chronically for 21 days with imipramine, 10 mg/kg, twice daily; with ECS (90 mA, 50 Hz, 0.5 s) every second day; or with haloperidol, 1.2 mg/kg, once daily. Appropriate controls were injected with saline. Rats were sacrificed 24 h after last treatment and their hippocampi were taken out for analysis. It was found that the mGluR1a-immunoreactivity expression increased significantly in Ammon's horn (CA) regions after chronic ECS. The most pronounced effect was observed in the CA3. No significant effects were found after single treatment or after haloperidol. The expression of mGluR5a increased significantly after chronic impiramine in the CA1 and after chronic ECS in the CA3 region. The results obtained indicate an influence of antidepressant treatment on group I mGluR. This increase in the receptor protein level may be a compensatory mechanism developing after chronic treatment.


    166/355

    Tytuł oryginału: Neuropharmacology
    Tytuł angielski: Synergistic effect of uncompetitive NMDA receptor antagonists and antidepressant drugs in the forced swimming test in rats.
    Autorzy: Rogóż Zofia, Skuza Grażyna, Maj Jerzy, Danysz Wojciech
    Źródło: Neuropharmacology 2002: 42 (8) s.1024-1030, il., bibliogr. s. 1029-1030
    Sygnatura GBL: 305,141

    Hasła klasyfikacyjne GBL:
  • psychiatria i psychologia
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • zwierzęta
  • szczury

    Streszczenie angielskie: In spite of intensive research, the problem of treating antidepressant-resistant depressive patients has not yet been solved. The authors previously reported that combined administration of imipramine and ther uncomperative NMDA receptor antagonist amantadine reduced immobility time in the swimming test in rats to a much greater extent than treatment alone. The present paper investigates the possibility of synergistic interactions between there antidepressants (imipramine, venlafaxine, fluoxetine) with three uncompetitive NMDA receptor antagonists (imatadine, memantine and neramexane). Most combinations resulted in synergistic (hyperadditive) antidepressive-like effects in the forced swim test. Most interesing was the observation that fluoxetine, which was inactive when given alone, showed a positive effect when combined with amantadine (10 and 20 mg/kg), memantine (2.5 and 5 mg/kg) or neramexane (2.5 and 5 mg/kg). The specificyty of these observations is supported by control open field studies, which demonstrated no significant increase, or even a decrease in general locomotion after coadministration of the compounds. The present results suggest that the combination of traditional antidepressant drugs and NMDA receptor antagonists may produce enhanced antidepressive effects, and this is of particular relevance for antidepressant-resistant patients.


    167/355

    Tytuł oryginału: Renal and ureteric trauma: diagnosis and management in Poland.
    Autorzy: Dobrowolski Z[ygmunt], Kusionowicz J., Drewniak T., Habrat W., Lipczyński W., Jakubik P., Węglarz W.
    Źródło: Br. J. Urol. 2002: 89 (7) s.748-751, tab., bibliogr. 14 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia
  • urologia
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective. To analyse retrospectively kidney and ureteric injuries (the former often associated with multipleorgan trauma) and thus optimize diagnostic and treatment methods. Patients and methods: the records and details of kidney and ureteric injures between 1995 and 1999 in 61 urological departments in Poland were analysed. Results. In all, 887 kidney injuries were analysed: blunt trauma comprised 97 p.c., with most injuries classified as renal contusion and minor parenchymal damage (687 cases). Intravenous urography was used in 80 p.c. of the patients and computed tomography in only 20 p.c. In all, 234 patients (26 p.c.) underwent surgery; nephrectomy was the most common surgical treatment, in 170 patients (73 p.c. of those undergoing surgery). Complications occured in 9 p.c. of patients after conservative treatment and in 5 p.c. after surgery. Of the 452 ureteric injuries, 340 (75 p.c.) were iatrogenic, 81 (18 p.c.) blunt injuries and 31 (7 p.c.) open injuries. Of the iatrogenic injuries 73 p.c.occurred during gynaecological procedures, 14 p.c. in general surgery and 14 p.c. in urological procedures. The most frequent diagnostic method was intravenous urography (244 cases), with retrograde pyelography (98) and ureteric catheterization in 125. The diagnosis was estabilished immediately during surgery in 104 patients. The most frequent surgical treatment was uretero-neocystostomy (213, 47 p.c.), the others being a Boari flap (113, 25 p.c.), end-to-end anastomosis (92, 20 p.c.), reconstruction with an ileal loop (30, 7 p.c.) and autotransplantation (four, 1 p.c.)...


    168/355

    Tytuł oryginału: Treatment of posterior and anterior urethral trauma.
    Autorzy: Dobrowolski Z[ygmunt] F., Węglarz W., Jakubik P., Lipczyński W., Dobrowolska B.
    Źródło: Br. J. Urol. 2002: 89 (7) s.752-754, bibliogr. 9 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective. To assess the occurence and treatment of posterior uretheral (most often caused by traffic accidents and comprising half of all cases of urinary tract traumas) and anterior uretheral injury (usually iatrogenic during catheterization or cystoscopy, or caused by blunt trauma, e.g. straddle injury or penetrating trauma). Patients and methods: The records and details of patients with posterior and anterior urethral trauma were analysed from 61 urological depatrments in Poland between 1995 and 1999. Results: During the 5-year period there were 268 cases of posterior and 255 of anterior uretheral injury; for the former, most occurred during traffic accidents, and of the latter 206 were iatrogenic (during catheterization or cystoscopy), of which 48 were accompanied by perineal injury. The posterior injuries were isoplated or involved many organs, mainly pelvic bones. Both types of injuries were diagnosed using ascending urethrography, voiding cysto-urethrography and urethroscopy/endoscopy. Treatment for posterior injuries included cystostomy alone or cystostomy with abdominal drainage or perineal drainage; some patients also had their urethra reconstructed. The treatment for anterior injuries was conservative in 193 patients and surgical in 62. Conclusion: The early detection and appropriate surgical treatment of posterior and anterior urethral injury is crucial for the recovery of urethral function, and avoids many complications.


    169/355

    Tytuł oryginału: External and iatrogenic trauma of the urinary bladder: a survey in Poland.
    Autorzy: Dobrowolski Z[ygmunt] F., Lipczyński W., Drewniak T., Jakubik P., Kusionowicz J.
    Źródło: Br. J. Urol. 2002: 89 (7) s.755-756, bibliogr. 3 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: To assess incidence of urinary bladder injuries (frequently associated with pelvic trauma and often iatrogenic) in Poland. Materials and methods: The records and details of urinary bladder injuries treated between 1995 and 1999 were analysed for 61 urological departments in Poland. Results: During the 5-year period 512 patients had urinry bladder injuries: in 210 (41 p.c.) the injury was caused by a road traffic accident, in eight (2 p.c.) by compression (crushing injury) within the limits of the pelvic bones, 40 (8 p.c.) by a fall from a height, in three by a gunshot wound and in teh remaining 251 (49 p.c.) the injury was iatrogenic. Among the 261 bladder injuries that were not iatrogenic, 41 (16 p.c.) were associated with pelvic bone trauma. In 36 patients there was simultaneous injury of the urinary bladder and posterior urethra, constituting 14 p.c. of such injuries and 7 p.c. of all trauma cases. The iatrogenic injuries were in 98 patients (39 p.c.) in urological depatrments, in 130 (52 p.c.) women in gynaecological departments and in 23 (9 p.c.) on surgical wards. The injury was open in 102 patients (20 p.c.) and closed in 372 (73 p.c.0; there was bladder contusion in 38 patients (7 p.c.). The injuries were intraperitoneal in 225 patients (44 p.c.) and extraperitoneal in 287 (56 p.c.). For diagnosis, abdominal ultasonography was used in 455 (89 p.c.) patients, intravenous pyelography in 266 (52 p.c.), cystography in 338 (76 p.c.) and comuted tomography in 15 (3 p.c.). The delay between trauma and diagnosis was ...


    170/355

    Tytuł oryginału: Comparison of the bladder tumour antigen test with photodynamic diagnosis in patients with pathologically confirmed recurrent superficial urinary bladder tumours.
    Autorzy: Lipiński M., Jeromin L.
    Źródło: Br. J. Urol. 2002: 89 (7) s.757-759, bibliogr. 20 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Objective: To verify the sensitivity of the bladder tumour antigen (BTAstat, Bard Urological, Convington, GA) test against the sensitive procedure of photodynamic diagnosis (PDD), in which 5-aminolaevulinic acid (5-ALA, a precursor of fluorescent porphyrins) is absorbed by the tumour and detected by ultraviolet cystoscopy, in the early diagnosis of urinary bladder tumours. Patients and methods: Forty-three patients (31 men and 12 women, age range 21-87 years) were assessed after transurethral resection of their bladder tumour using the BTAstat test and PDD. Sixty-nine biopsies from suspect areas of bladder mucosa were taken during cystoscopy under ultraviolet light and all suspect lesions electrocoagulated. Results: Thirty-five patients (81 p.c.) had a positive BTAstat test; in these patients PDD detected malignant lesions (17 Ta1G1-2, two T1G2, two T1G3 and 14 Tis). In eight patients (19 p.c.) the BTAstat was negative but PDD detected three malignant lesions (two Tis and one TaG1). Conclusions: PDD is valuable for detecting bladder malignancy and can identify small lesions not detected by the BTAstat test.


    171/355

    Tytuł oryginału: Transurethral microwave thermotherapy: first experiences in Poland.
    Autorzy: Jakubczyk T., Stokłosa A[ndrzej], Borkowski A[ndrzej], Habrat W., Dobrowolski Z[ygmunt F.]
    Źródło: Br. J. Urol. 2002: 89 (7) s.767-770, tab., bibiliogr. 21 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • onkologia
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: Objective: To present early experiences in Poland with high-energy transurethral microwave thermotherapy (HeTUMT), considered as a serious alternative to surgery in managing benign prostatic hypertrophy (BPH). Patients and methods: Sixty-one patients were treated in two centres using a commercial TUMT system. The patients were assessed at 5-6 weeks, 3 and 6 months after treatment, evaluating subjective complaints, the International Prostate Symptom Score (IPSS), urinary flow rate and postvoid residual volume. Results: A complete follow-up was available in 44 patients; most had symptomatic improvement 3 months after HeTUMT but it was slightly less pronounced after 6 months. The improvement in objective variables increased up to 6 months after HeTUMT. There was ň 50 p.c. improvement in subjective complaints in 43 p.c. of the patients, in urinary flow rate in 25 p.c. and in residual urine in half. Two patients required re-treatment for a urethral stricture and ineffective HeTUMT. Conclusions: HeTUMT may be a useful treatment for BPH but the outcome for an individual patient seems to be unpredictable. The indications for TUMT should be reviewed individually for each patient.


    172/355

    Tytuł oryginału: The efficacy of terazosin for treating benign prostatic hyperplasia: a multicentre clinical trial.
    Autorzy: Miękoś E[ugeniusz], Trzepizur Z., Różański W., Boniecki R., Kuśnierz J., Jabłonowski Z., Zydek C.
    Źródło: Br. J. Urol. 2002: 89 (7) s.771-772, tab., bibliogr. 7 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • urologia
  • toksykologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska


    173/355

    Tytuł oryginału: The therapy of benign prostatic hyperplasia using less-invasive procedures: the current situation.
    Autorzy: Pomer S., Dobrowolski Z[ygmunt] F.
    Źródło: Br. J. Urol. 2002: 89 (7) s.773-775, bibliogr. 14 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • urologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    174/355

    Tytuł oryginału: Microangiographic imaging of the prostate.
    Autorzy: Słojewski M[arcin], Czerwiński F[lorian], Sikorski A[ndrzej]
    Źródło: Br. J. Urol. 2002: 89 (7) s.776-778, il., bibliogr. 11 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: To present the results of microangiography in visualizing prostatic microvascularization. Materials and methiods: A contarst medium was injected into the vessels of prostate specimens obtained during surgery, to fill small vessels; nine specimens were obtained after cystoprostatectomy and two after radical prostatectomy. Branches of prostatic or seminal vesicle arteries were used for the injection, the specimens then fixed in formalin, sectioned and X-rayed. Results: In five samples the quality of the images was sufficient to visualize small arteries; the remaining vessels were partially filled. The vascular map was compared with previous results from historadiographic studies. Conclusions: Microangiographic imaging of prostate specimens provides a unique view of the angioarchitecture of the prostate and correlates closely with historadiographic studies. The correlation with other imaging methods, e.g. Doppler ultrasonography, needs further evaluation.


    175/355

    Tytuł oryginału: Usefulness of power Doppler ultarsonography in evaluating erectile dysfunction.
    Autorzy: Golubiński A. J., Sikorski A[ndrzej]
    Źródło: Br. J. Urol. 2002: 89 (7) s.779-782, il., bibliogr. 16 poz.
    Sygnatura GBL: 300,816

    Hasła klasyfikacyjne GBL:
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska

    Streszczenie angielskie: Objective: To evaluate deep penile arterial flow after an intracavernosal injection with papaverine in patients with erectile dysfunction (ED). Patients and methods: Twenty patients with ED were evaluated using power Doppler ultrasonography with a linear probe (8 MHz). Diagnostic tests were undertaken after an intracavernosal injection with 40 mg papaverine. The peak systolic velocity (PSV), enddiastolic velocity (EDV) and resistive index (RI) were analysed. Results: After injecting papaverine, seven patients had a normal erection and appropriate waveform patterns; their mean PSV was 30.7 cm/s, the EDV 4.42 cm/s and the RI 0.85. There was tumescence and elongation of the penis with no rigidity in eight patients; their mean PSV was 23.9 cm/s, the EDV 7.34 cm/s and the RI 0.72. There was no erection in five patients. The abnormal flow values showed insufficient arterial vessels in a quarter of the men, venous leakage in 15 p.c. and mixed ED in 20 p.c. Conclusion: The power Doppler technique allows the accurate location and evaluation of deep penile arteries. Vascular pathology may be differentiated after an intracavernosal injection with a vasomotor agent. Recognising the pathological pattern assists in choosing the best method of treatment.


    176/355

    Tytuł oryginału: Brain Research
    Tytuł angielski: Expression and kinetic properties of Na+ currents in rat cardiac dorsal root ganglion neurons.
    Autorzy: Rola Rafał, Szulczyk Bartłomiej, Szulczyk Paweł, Witkowski Grzegorz
    Źródło: Brain Res. 2002: 947 (1) s.67-77, il., tab., bibliogr. 36 poz.
    Sygnatura GBL: 312,085

    Hasła klasyfikacyjne GBL:
  • kardiologia
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The expression and properties of voltage-gated Na+ currents in cardiac dorsal root ganglion (DRG) neurons were assessed in this study. Cardiac DRG neurons were labelled by injecting the Fast Blue fluorescent tracer into the pericardium. Recordings were performed from 138 cells. Voltage-dependent Na+ currents were found in 115 neurons. There were 109 neurons in which both tetrodotoxin-sensitive (TTX-S, blocked by 1 ćM of TTX) and tetrodotoxin-resistant (TTX-R, insensitive to 1 ćM of TTX) Na+ currents were present. Five cells expressed TTX-R current only and one cell only the TTX-S current. The kinetic properties of Na+ currents and action potential waveform parameters were measured in neurons with cell membrane capacitance ranging from 15 to 75 pF. The densities of TTX-R (110.0 pA/pF) and TTX-S (126.1 pA/pF) currents were not significantly different. Current threshold was significantly higher for TTX-R (-34 mV) than for TTX-S (-40.4 mV) currents. V1/2 of activation for TTX-S current (-19.6 mV) was significantly more negative than for TTX-R current (-9.2 mV), but k factors did not differ significantly. V1/2 and the k constant for inactivation for TTX-S currents were -35.6 and -5.7 mV, respectively. These values were significantly lower than those recorded for TTX-R current for which V1/2 and k were -62.3 and -7.7 mV, respectively. The action potential threshold was lower, the 10-90 p.c. rise time and potential with were shorter before than after the application of TTX. Based on this we drew the conclusion that action potential recorded before adding tetrodotoxin was mainly...


    177/355

    Tytuł oryginału: Midfacial fractures in children.
    Autorzy: Kos M., Łuczak K., Godziński J., Rapala M., Klempous J.
    Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.218-225, il., tab., bibliogr. 20 poz., r‚s., res., Zsfg
    Sygnatura GBL: 312,942

    Hasła klasyfikacyjne GBL:
  • pediatria
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The purpose of the study was to present a modern classification and discuss the treatment of midfacial fractures in children. From the beginning of 1 January 1998 to 31 October 2000, 147 children were treated for different craniofacial fractures. Among them 28 patients (19 p.c.) had extensive midfacial fractures complicated by impaired vision and/or CNS dysfunction and were treated surgically. The fractures were divided into: zygomaticoorbital (1 pt) and zygomatico-orbito-maxillary (5 pts), isolated orbital wall fractures (14 pts) and naso-orbital dislocations (2 pts), upper facial portion dislocations (2 pts) and fronto-naso-orbital (2 pts) or cranio-orbital fractures (2 pts). Clinical examination revealed mainly dysfunction of facial morphology and aesthetics (enophthalmos, telecanthus), CSF leakage, impairment of vision and restricted eyeball movements. Ophthalmological, neurological and radiological (Waters view, CT, NMR) examinations were performed in all cases. The treatment consisted of bone repositioning, reconstruction with autologus bone grafts and three-dimensional stabilisation using titanium plates. Twenty-three pts were operated on before and 5 pts after 7th day after trauma. The restoration of normal facial morphology was achieved in 26 patients. In 2 cases it was imposisible because of the destruction of soft tissue and an extremely extensive bony trauma. Better reposition of bony fragments, prompt healing and better final results were achieved in patients operated before day 7 after trauma. Achieving good results in midfacial fractures requires prompt...


    178/355

    Tytuł oryginału: Minimally invasive repair of pectus excavatum - the nuss procedure. A European Multicentre Experience.
    Autorzy: Hosie S., Sitkiewicz T., Petersen C., G”bel P., Schaarschmidt K., Till H., Noatnick M., Winiker H., Hagl C., Schmedding A., Waag K.-L.
    Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.235-238, il., tab., bibliogr. 5 poz., r‚s., res., Zsfg
    Sygnatura GBL: 312,942

    Hasła klasyfikacyjne GBL:
  • chirurgia
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny
  • praca opublikowana za granicą

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Since the first description in 1998, the minimally invasive reapir of pectus excavatum has gained increasing acceptance. The aim of this survery is to report on the experiences of eight European centres with this technique. 172 patients with a mean age of 15.1 (ń4.6) years were treated and evaluated, 35.5 p.c. were sympomatic. 45.3 p.c. of the patients had an asymmetric configuration of the chest, 74.3 p.c. had a CT index above 3.25. Mean duration of the operative procedure was 76 minutes. Major complications, including dislocation of the bar or stabiliser, pneumonia, atelectasis, local infection, pleural and pericardial effusion and liver injury occurred in 11.1 p.c. of the patients. Minor complications, such as self-resolving pneumothorax, atelectasis and subcutaneous emphysema were reported in another 8.1 p.c. Early cosmetic results were excellent or good in 81.5 p.c. Although the surgical procedure is simple, blood-sparing and short, consideration of some important technical details, proper patient selection and knowledge of the limitations is of vital importance. Long-term results are still lacking.


    179/355

    Tytuł oryginału: Perspetives of clinical application of bone morphogenetic proteins in children.
    Autorzy: Kos M., Łuczak K., Matusiewicz M., Krzystek-Korpacka M., Rapala M.
    Źródło: Eur. J. Pediatr. Surg. 2002: 12 (4) s.272-277, il., bibliogr. 20 poz., r‚s., res., Zsfg
    Sygnatura GBL: 312,942

    Hasła klasyfikacyjne GBL:
  • traumatologia i ortopedia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • bydło
  • szczury

    Streszczenie angielskie: The purpose of the study was to modify the method of Bone Morphogenetic Protein (BMP) extraction from bovine bones, estimate the osteoinductive potential of the extracted protein fractions in relation to the degree of protein purity and to test collagen formed in porous discs as a carrier. It was required before BMP application in reconstructive surgery in children. Material and method: BMP preparation from bovine bones was conducted according to a modification of Luyten's method. Three fractions of different degree of purity - demineralised bone matrix "M", extract "E", and fraction after hydroxyapatite column "HP" - were tested for their osteoinductive potential. They were implanted on a collagen carrier into the right rat femoral muscle pouch. Controls were implanted in the same way in the left muscle pouch. The discs were removed after 3 and 6 weeks and tested for alkaline phosphatase activity, a marker of new bone formation. Results: Out of the 10 animals used for testing the osteoinductive potential of matrix fraction, 7 results were positive and 3 negative. In the case of the extract fraction, the analysis was conducted for 3 weeks on 9 rats and 6 weeks on 13 rats. Six and 11 positive results were obtained, respectively. In the HP group 5 animals were observed for 3 weeks (3 positive, 2 negative) and 10 animals for 6 weeks (7 positive, 3 negative). In the case of the extract, the differences in alkaline phosphatase activity after 6 weeks were statistically significant (p ó 0.05) compared to controls. In the other groups, a positive tendency was observed...


    180/355

    Tytuł oryginału: Effect of NADH on the redox state of human hemoglobin.
    Autorzy: Olek Robert A., Antosiewicz Jędrzej, Caulini Gian Carlo, Falcioni Giancarlo
    Źródło: Clin. Chim. Acta 2002: 324 (1/2) s.129-134, il., bibliogr. 15 poz.
    Sygnatura GBL: 303,881

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismuatase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced, in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involvews free radical species.


    181/355

    Tytuł oryginału: Effect of Helicobacter pylori on delay in ulcer healing induced by aspirin in rats.
    Autorzy: Konturek Peter C., Brzozowski Tomasz, Kwiecień Sławomir, Drozdowicz Danuta, Harsch Igor A., Meixner Holger, Stachura Jerzy, Hahn Eckhart G., Konturek Stanisław J.
    Źródło: Eur. J. Pharmacol. 2002: 451 (2) s.191-202, il., tab., bibliogr [37] poz.
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • mikrobiologia
  • gastroenterologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs) are major pathogenic factors in peptic ulcer disease but whether these two factors exert synergistic or antagonistic effects on ulcer healing has been a subject of controversy. We compared the effect of aspirin alonne with that of aspirin combined with H. pylori on gastric acid secretion and healing of acetic gastric ulcers in rats. The H. pylori colonization of gastric mucosa was determined by viable H. pylori culture, histology and assessment of bacterial DNA using polymerase chain reaction (PCR). The area of ulcers, gastric blood flow, mucosal generation of prostaglandin E2 and plasma gastrin levels and expression of cyclooxygenase-1, cyclooxygenase-2 and growth factors was determined. Aspirin delayed significantly the healing of chronic gastric ulcers, decreased the gastric blood flow at the ulcer margin and gastric mucosal prostaglandin E2 heneration being without significant influence on gastric acid output H. pylori acquisition that produced moderate gastric inflammation at the ulcer margin delayed significantly the healing of gastric ulcers, decreased significantly both the gastric blood flow at the ulcer margin and the gastric secretion while raising significantly the gastric mucosal prostaglandin E2 generation and plasma gastrin levels. H. pylori infection attenuated the aspirin-induced inhibition of ulcer healing and accompanying fall in the gastric blood flow. Both aspirin and H. pylori up-regulated significantly cyclooxygenase-2 messenger RNA (mRNA)...


    182/355

    Tytuł oryginału: Progastrin and cyclooxygenase-2 in colorectal cancer.
    Autorzy: Konturek Peter C., Bielański Władysław, Konturek Stanisław J., Hartwich Artur, Pierzchalski Piotr, Gonciarz Maciej, Marlicz Krzysztof, Starzyńska Teresa, Zuchowicz Monika, Darasz Zbigniew, G”tze Jens P., Rehfeld Jens F., Hahn Eckhart G.
    Źródło: Dig. Dis. Sci. 2002: 47 (9) s.1984-1991, il., tab., bibliogr. 41 poz.
    Sygnatura GBL: 310,037

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Colorectal cancers (CRCs) are one of the most common forms of cancer in Poland and one of the leading causes of dearh. The tumors have been attributed to genetic, dietary, and other environmemtal factors, but recently factors such as gastrin have also been implicated in the carcinogenesis. The relationship between plasma amidated and nonamidated gastrin in CRCs is controversial. This study was designed to determine the plasma levels of progastrin and amidated gastrin in 50 CRC patients before and 3-6 months after removal of the tumor, to determine the tumor concentrations of these gastrin peptides and the level of expression for gastrin mRNA and gastrin/CCKb receptor mRNA, to examine the expression of cyclooxygenase COX-1 and COX-2 in mRNA in CRC tissue, and to compare the prevalence of Hp and its cytotoxic protein, CagA, and cytokines (TNFŕ, IL-1á, and IL-8) in CRCs, before and after removal of tumor. It was found that thwe CRC, its resection margin, and the plasma contained severalfold higher levels of progastrin than of amidated gastrins and that the removal of the CRC tumor resulted in a marked reduction in plasma progastrin level without a significant alteration in plasma levels of amidated gastrins. Both gastrin and CCKb-R mRNA were detected in the cancer tissue and resection margin by RT-PCR, and similary, COX-1 and COX-2 mRNA were expressed in these tissues of moast CRCs. The seroprevalence of Hp, especially that expressing CagA, and levels of IL-1á, but not other cytokines, were significantly higher in CRC patients than in 100 age-...


    183/355

    Tytuł oryginału: Multiple MPEP administrations evoke anxiolytic- and antidepressant-like effects in rats.
    Autorzy: Pilc A., Kłodzińska A., Brański P., Nowak G., Pałucha A., Szewczyk B., Tatarczyńska E., Chojnacka-Wójcik E., Wierońska J. M.
    Źródło: Neuropharmacology 2002: 43 (2) s.181-187, il., tab., bibliogr. s. 186-187
    Sygnatura GBL: 305,141

    Hasła klasyfikacyjne GBL:
  • psychiatria i psychologia
  • farmacja
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć męska

    Streszczenie angielskie: Several lines of evidence suggest a crucial involvement of glutamate in the mechanism of action of anxiolytic and antidepressant drugs. The involvement of group I mGLu receptors in anxiety and depression has also been proposed. In view of the recent discovery of anxiolytic- or antidepressant-like effects of acute injections of 2-methyl-6-(phenylethyl)-pyridine (MPEP), a selective and brain penetrable mGlu5 receptor antagonist, we designed the present study to examine anxiolytic- and/or antidepressant-like effects of multiple administration of this drug. The anxiolytic-like effects of MPEP were evaluated in rats using the conflict drinking test. The antidepressant-like effect was estimated using the rat olfactory bulbectomy model of depression. Seven subsequent injections of MPEP (1 mg/kg) significantly (by 320 p.c.) increased the number of shock accepted during the experimental session in the Vogel test. MPEP given once daily at a dose of 10 mg/kg, restored the learning deficit of bulbectomized rats after 14 days of treatment, remaining without any effect in the sham-operated animals. N-methyl-D-aspartic acid (NMDA)-induced convulsions in mice were not affected by a single injection of MPEP (30 mg/kg) indicating that at this dose MPEP did not block NMDA receptors. The results indicate that the prolonged blockade of mGlu5 receptors exert anxiolytic- and antidepressant-like effects in rats. No tolerance to anxiolytic-like action occurs. The previously mentioned results further indicate that antagonists of group I mGlu receptors may play a role in the therapy of both anxiety and depression.


    184/355

    Tytuł oryginału: Papilomavirusines infekcijos paplitimas tarp ligoniu, serganciu gerklu papilomatoze, ir kai kuriu rizikos veiksniu itaka papilomavirusu persistavimui virsutiniuose kvepavimo takuose.
    Tytuł angielski: Prevalence of human papillomavirus infection among patients with laryngeal papillomatosis and the influence of some risk factors on the persistence of papillomaviruses in the upper respiratory tract.
    Autorzy: Velyvyte Daiva, Laiskonis Alvydas, Uloza Virgilijus, Goździcka-Józefiak Anna
    Źródło: Medicina 2002: 38 (5) s.499-504, tab., bibliogr. 19 poz., sum.
    Sygnatura GBL: 313,016

    Hasła klasyfikacyjne GBL:
  • pulmonologia
  • onkologia
  • otorynolaryngologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Infection with high-risk human papillomaviruses is a significant risk factor of various benign and malignant human lesions in the upper respiratory tract, skin and the genital tract. The identification of particular human papillomaviruses types is important fir identifying patients with premalignant lesions who are at risk of progression to malignancy. Our aim was to establish the prevalence of human papillomaviruses infection in the upper respiratory tract of patients with laryngeal papillomatosis, to identify viral types, to evaluate the relationship between some risk factors and persistence of human papillomviruses in the upper respiratory tract and to determine the pattern of human papilomaviruses infection. Material and methods. The gorup fo 36 patients with laryngeal papillomatosis and control group of 108 persons without any complains of respiratory system was examined. Epidemiologic characteristics and objective data were analyzed and routine laryngological examination was performed. Pharyngeal swabs of all persons and laryngeal biopsies of 17 patients were taken and analyzed for the presence fo human papillomaviruses DNA. Viral typing using the polymerase chain reaction was performed. Results. Human papillomaviruses DNA was detected in all except one case of laryngeal papillomatosis; then only 23.15 p.c. of persons without complaints of respiratory system were found human papillomaviruses positive. Human papillomaviruses 6, 11 types were predominant (in 88.9 p.c. of patients adn 19.4 p.c. of persons from control gorup). High-risk human papillomaviruses were detected in 52.78 p.c. of laryngeal papillomatosis cases and in 9.26e p.c. of control cases. Risk factors were noted statistically significantly more often in human papillomaviruses positive cases...


    185/355

    Tytuł oryginału: Emotional changes related to age in rats - a behavioral analysis.
    Autorzy: Boguszewski Paweł, Zagrodzka Jolanta
    Źródło: Behav. Brain Res. 2002: 133 (2) s.323-332, il., tab., bibliogr. 39 poz.
    Sygnatura GBL: 312,662

    Hasła klasyfikacyjne GBL:
  • neurologia
  • psychiatria i psychologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The present study investigated age-related differences in the emotional behavior of rats using factor analysis to identify motivational factors influencing spontaneous behavior in open field with illuminated center (OF), plus maze (EPM) and social interactions test. Animals of the same strain, bred under the same conditions, formed two experimental groups: yound adults (YA, N - 20) tested at the age of 4 months and old rats (OA N = 16) tested at the age of 24 months. The comuter video based tracking system Etho Vision was used for automated acquisition and analysis of data. The reslults of each test were analyzed separately for YA and OA by factor analysis. Two main independent factors emerged from the analysis of OF measures - factor 1, which appeared to reflect motor activity, and factor 2, reflecting anxiety. The measures best reflecting motor activity (distance moved in the peripheral zone) and anxiety (time spent in central zone) decreased significantly with age. Factor analysis for EPM measures revealed, in both groups, three independent factors. In YA, factor 1 reflected motor activity, factor 2 - anxiety, in OA measures of anxiety loaded on factor 1, measures of activity on factor 2. Factor 3 in both groups appeared to represent a decision making process. The number of entries to the closed arms declined significantly in OA, showing an age related decrease of motor activity. Also, the ratio of open arms entries in relation to the total number of entries decreased in OA, indicating a higher anxiety level...


    186/355

    Tytuł oryginału: Obesity-related hypertension: relevance of vascular responses to mental stress.
    Autorzy: Narkiewicz Krzysztof
    Źródło: J. Hypertens. 2002: 20 (7) s.1277-1278, bibliogr. 21 poz.
    Sygnatura GBL: 313,132

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • kardiologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    187/355

    Tytuł oryginału: Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea.
    Autorzy: Paciorek Jarosław
    Źródło: J. Med. Microbiol. 2002: 51 (7) s.548-556, il., tab., bibliogr. 58 poz.
    Sygnatura GBL: 305,350

    Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • gastroenterologia
  • pediatria

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny
  • praca kliniczna

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Four hundred and twetny-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enteroheamolysin, the genes encoding intimin (eae), bundle-forming pili (bfp), Shiga toxins I and II (stxI, stxII) and cytotoxic necrotising factor types 1 and 2 (cnf1, cnf2) was investigated by PCR. Adhesion to HEp-2 cell monolayers was also tested and selected strains were investigated for the presence of P-fimbriae and haemolytic activity. Typical enteropathogenic E. coli isolates (typical EPEC, strains possessing eae and bfp, but not stx) were not found. The particular classes of E. coli among 427 isolates from ill children were: atypical EPEC (eae**+ bfp, stx**-), 21.3 p.c.; Shiga toxin-producing E. coli (STEC), 0.7 p.c.; diffsely adherent E. coli (DAEC), 4 p.c.; enteroaggreagative E. coli (EAEC), 16.9 p.c.; necrotoxic E. coli type 1 (NTEC1), 0.2 p.c.; and cell-detaching E. coli (CDEC), 29 p.c. With the exception of STEC, all the above classes of E. coli were found among the isolated from healthy children which comprised: atypical EPEC 8.0 p.c., DAEC 6.7 p.c.; EAEC 17.3 p.c., NTEC1 14.0 p.c. and CDEC 40.0 p.c. Cell detachment (CD) was significantly associated with 3-h haemolytic activity. There was also strong correlation between haemolytic activity (Hly) and the presence of p-fimbriae. No correlation was found between the presence of te cnf1 gene and CD, Hly or P-fimbriae.


    188/355

    Tytuł oryginału: Cytokine serum levels in soft tissue sarcoma patients: correlations with clinico-pathological features and prognosis.
    Autorzy: Rutkowski Piotr, Kamińska Janina, Kowalska Maria, Ruka Włodzimierz, Steffen Jan
    Źródło: Int. J. Cancer 2002: 100 (4) s.463-471, il., tab., bibliogr. 85 poz.
    Sygnatura GBL: 305,029

    Hasła klasyfikacyjne GBL:
  • onkologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny
  • praca kliniczna

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.

    Streszczenie angielskie: We investigated the correlations between serum levels of selected proinflammatory, hematopoietic and angiogenic cytokines and soluble cytokine receptors with the clnicopathological features and prognosis in soft tissue sarcom patients. Serum levels of 9 cytokines (TNFŕ, IL-Ira, IL-6, IL-8, IL-10, M-CSF, G-CSF, VEGF, bFGF) and 4 free cytokine receptors (sIL-2Rŕ, sIL-6R, TNFRI TNFRII) were measured by means of an enzyme-linked immunoadsorbent assay kit in 156 soft tissue sarcoma patients before the treatment and in 50 healthy controls. Serum levels of 10 cytokines and cytokine receptors were also assayed during patients' follow-up after the treatment. Significantly elevated pretreatment serum levels of 11/13 cytokines and cytokine receptors were found in sarcoma patients, as compared to healthy controls. In 40.4 p.c. of patients 6 or more cytokines and cytokine receptors (most frequently: TNF RI, IL-6, IL-8) were elevated in parallel. Serum levels of IL-6, sIL-2R, VEGF, M-CSF and TNF RI correlated significantly with tumor size and serum levels of IL-8 and IL-6 were significantly higher in patients with Grade 2/3 vs. Grade I tumors. We did not observe any significant differences in cytokine serum levels between patients with primary and recurrent tumors and patients with and without distant metastases. Using univariate analysis, overall survival (OS) in all patients was affected by tumor size ( 5 cm vs. 5-10 cm vs. 10 cm), tumor grade (GI vs. G2/3), presence of metastases, pretreatment serum levels of 8 cytokines...


    189/355

    Tytuł oryginału: Ultrastructural observations of intermediate cells in chronic pancreatitis.
    Autorzy: Szynaka Beata, Zimnoch Lech, Puchalski Zbigniew
    Źródło: Hepatogastroenterology 2002: 49 (46) s.1120-1123, il., bibliogr. 28 poz.
    Sygnatura GBL: 304,355

    Hasła klasyfikacyjne GBL:
  • gastroenterologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Background/Aims: The intermediate cells exibiting both exo- and endocrine features occur in small numbers in the normal and pathologic pancreas of many animal species. In human pathology their presence has ben reported in children with hyperinsulinemia, in hypoglycemia, and chronic hypergastrinemia. Methodology: Fragments of the pancreas collected from 27 patients operated on for chronic pancreatitis of various intensity fibrosis (1ř - IVř) was subjected to ultrastructural analysis. Results: All chronic pancreatitis cases revealed the presence of intermediate cells that were found outside Langerhans islets, separately or in small groups. They were more common in IIIř and IVř chronic pancreatitis. All forms of chronic pancreatitis with Iř - IVř fibrosis showed, apart from undamage cells, numerous intermediate cells with features of destruction. Some intermediate cells contained fibrilate cytoplasm inclusion bodies. Intermediate cells took part in the formation of acini and were also found among ductural cells. Conclusions: The presence of intermediate cells both within the acinar texture and among proliferating ductular cells conforms the common origin of these cellular elements of the pancreas. In chronic pancreatitis intermediate cells appear during pancreatic texture regeneration, being rather abnormal cell forms produced in this process than an intermediate stage in precursor cell diferentiation.


    190/355

    Tytuł oryginału: Mast cells and pancreatic stellate cells in chronic pancreatitis with differently intensified fibrosis.
    Autorzy: Zimnoch Lech, Szynaka Beata, Puchalski Zbigniew
    Źródło: Hepatogastroenterology 2002: 49 (46) s.1135-1138, il., tab., bibliogr. 15 poz.
    Sygnatura GBL: 304,355

    Hasła klasyfikacyjne GBL:
  • gastroenterologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Background/Aims: The pathogenesis of pancreatic fibrosis is unknown. Pathogenic analyses take into account the effect of oxidant stress and the increase in free radicals leading to degranulation of mast cells, which may cause inflammation and activate fibrosis. Activated pancreatic stellate cells produce abnormal components of intercellular substance and are responsible for pancreatic fibrogenesis. The aim of the study was to determine the correlation between the presence and state of mast cells and pancreatic stellate cells activation in chronic pancreatitis with varied intensity fibrosis. Methodology: We studied 27 patients with chronic pancreatitis of varied fibrosis intensity. Immunohistochemical reactions for mast cells (anti-human mast cell tryptase) and ultrastructural analyses of mast cells and pancreatic stellate cells were performed. The number of degranulated mast in the ultrastructural picture and the number of pancreatic stellate cells stained positive for vimentin and ŕ-smooth muscle actin were counted. Results: A significant increase was revealed in the number of degranulated mast cells - to 35.6 p.c. in chronic pancreatitis Iř fibrosis, 68.3 p.c. in IIř, 75.1 p.c. in IIIř and IVř (control 10.2 p.c.) and a parallel increase in the number of activated pancreatic stellate cells (stained positive for ŕ-smooth muscle actin) to 328 ń 29/mmý in Iř fibrosis, to 978 ń 67/mmý in IIř and 2355 ń 331/mmý in IIIř and IVř (control 38.8 ń 9/mmý). Conclusions: The increase in the number of activated pancreatic stellate cells parallel to the increase in degranulated...


    191/355

    Tytuł oryginału: Plasmin/alpha2-antiplasmin complexes (PAP) in lung cancer patients: effects of radiotherapy.
    Autorzy: Uszyński Mieczysław, Ziółkowska Ewa, Żekanowska Ewa, Uszyński Waldemar
    Źródło: Thromb. Haemost. 2002: 88 (1) s.169-170, il., bibliogr. 2 poz.
    Sygnatura GBL: 304,333

    Hasła klasyfikacyjne GBL:
  • pulmonologia
  • onkologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • list i notatka
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska


    192/355

    Tytuł oryginału: The inhibitory effect of the fungicides captan and captafol on eukaryotic topoisomerases in vitro and lack of recombinagenic activity in the wing spot test of Drosophila melanogaster.
    Autorzy: Rahden-Staroń Iwonna
    Źródło: Mutat. Res 2002: 518 (1/2) s.205-213, il., tab., bibliogr. 53 poz.
    Sygnatura GBL: 304,952

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • in vitro

    Streszczenie angielskie: In studies on the mechanisms of mutagenic and carcinogenic action of captan and captafol-related chloroalkylthiocarboximide fungicides, two effects were tested: (i) the effect of both compounds on the activity of eukaryotic topoisomerase I and II in vitro, and (ii) their mutagenic and recombinagenic activity in the somatic mutation and recombination test (SMART) in wing cells of Drosophila melanogaster. Only captofal inhibited the activity of topoisomerase I (10-20 p.c. inhibition of activity in the range of 10-100 ćM). In contrast, both chemical decreased the activity of topoisomerase II already at 1 ćM concentration (50 and 20 p.c. inhibition of activity by captafol and captan, respectively). Genotoxicity was tested in vivo by administrating both compounds by acute (3 h) and chronic feeding (48 h) of 3-day-old exposure concentration range. Both chemical were inconclusive for large single spots, as well as for twin spots. In chronic treatment, captan showed positive results only for small single and total spots at 2.5 and 5 mM concentrations. Captafol gave inconclusive results over all concentrations tested. The results of the acute treatment experiments which have been performed at very high doses (50 p.c. toxicity at higher doses) indicate very weak overall mutagenic activity of both test fungicides.


    193/355

    Tytuł oryginału: Urinary tract infections caused by endemic multi-resistant Enterobacter cloacae in a dialysis and transplantation unit.
    Autorzy: Kamińska W., Patzer J., Dzierżanowska D.
    Źródło: J. Hosp. Infect. 2002: 51 (3) s.215-220, il., tab., bibliogr. 18 poz.
    Sygnatura GBL: 306,296

    Hasła klasyfikacyjne GBL:
  • pediatria
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.

    Streszczenie angielskie: In the period 1994-1998, 42 multi-resistant Eneterobacter cloacae isolates responsible for urinary tract infections (UTIs) were obtained from children hospitalized in our dialysis and transplantation unit. E. cloacae isolates obtained between 1994 and 1996 were susceptible to aminoglycosides, carbapenems and fluoroquinolones, whereas E. cloacae isolates obtained between 1997 and 1998 were susceptible to carbapenems and fluoroquinolones, only. All isolates were characterized by use of the polymerase chain reaction-based random amplification of polymorphic DNA and pulsed-field gel electrophoresis. It was found that the majority of multiresistant E. cloacae isolates obtained during 1997-1998, and all isolates obtained during 1994-1996, belonged to predominant clones A or B. These strains were responsible for gastrointestinal tract colonization and UTI of renal transplant recipients for several years, and persisted as endemic E. cloacae strains in the dialysis and transplantation unit from 1994 to 1998.


    194/355

    Tytuł oryginału: The relationship between gastric cancer cells circulating in the blood and microsatellite instability positive gastric carcinomas.
    Autorzy: Czopek J., Bialas M., Rudzki Z., Zazula M., Pituch-Noworolska A., Zembala M., Popiela T., Kulig J., Kołodziejczyk P., Stachura J.
    Źródło: Aliment. Pharmacol. Ther. 2002: 16 suppl. 2 s.128-136, il., tab., bibliogr. 31 poz. - 9 Międzynarodowe Sympozjum nt. gastroenterologii Shimoda 26-27.04. 2001
    Sygnatura GBL: 306,346

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Bacground: Cancers characterized by microsatellite instability may be biologically different from their counterparts with stable microsatellite sequences. Circulating cancers cell present in blood prior to surgery may constitute an adverse prognostic finding. Aim: To correlate these two phenomena with morphological features and survival in advanced gastric cancer. Methods: We examined 76 cases of resected sporadic, advanced gastric cancer by means of routine morphology and a panel of microsatellite markers. Sixty-six cases were screened for presence of cancer cells circulating in blood prior to the surgery using combined morphological and immunocytochemical approach. Results: Twenry-one (27.6 p.c.) cases demonstrated microsatellite instability in at least one locus. Among them 11 (14.5 p.c. showed microsatellite instability in more than 30 p.c. (4/12) examined loci, and they were therefore designated as replication error positive (RER+). Circulating cancer cells were detected in 2/19 microsatellite instability and in 11/47 remaining cases (difference not significant). The survival of the microsatellite instability cases was significantly better. The presence of circulating cancer cells did not correlate with survival. Conclusion: It is possible that the microsatellite instability status, but not circulating cancer cells, constitutes a prognostic predictive factor in advanced gastric carcinoma. Confirmation of this hypothesis requires continuation of patient follow-up.


    195/355

    Tytuł oryginału: Enrichment of normal progenitors in counter-flow centrifugal elutriation (CCE) fractions of fresh chronic myeloid leukemia leukapheresis products.
    Autorzy: Dłubek Dorota, Dybko Jarosław, Wysoczańska Barbara, Laba Anna, Klimczak Aleksandra, Kryczek Ilona, Konopka Lech, Lange Andrzej
    Źródło: Eur. J. Haematol. 2002: 68 (5) s.281-288, il., tab., bibliogr. 20 poz.
    Sygnatura GBL: 304,912

    Hasła klasyfikacyjne GBL:
  • hematologia
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.

    Streszczenie angielskie: Objectives: The aim of this study was to assess the suitability of a technique based on counter-flow centrifugal elutriation (CCE), which should allow one to enrich chronic myeloid leukemia (CML) patients' unstimalated native leukapheresis product (nLP) in CD34+ HLADR cells and BCR-ABL negative cells. Methods: Six newly diagnosed CML patients were subjected to leukapheresis, and the products were subfractionated with the use of CCE. nLP and all fractions were studied for the presence of CD34+ cells and a proportion of BCR-ABL fluorescence in situ hybridization (FISH)+ cells. Results: CCE fractions with a high flow rate contained the highest proportion of CD34+ cells [mean (SEM) 6.89 p.c. (3.88)]. However, CD34+ cells present in low-rate CCE fractions showed a higher proportion of HLADR- [49.6 p.c. (13.5 in 70 mL min -1) and 21.5 p.c. (11.6 in 110 mL min-1)] than those in 170 mL min-1 [3.2 p.c.(2.5)] and 'rotor off'[3.4 p.c. (1.9)]. This was associated with lower proportions of BCR-ABL FISH+ [8.1 p.c. (4.8) and 1.9 (1.7)] and smaller BCR-ABL to ABL transcript ratios [0.58 (17) and 0.26 (0.08) in 70 and 110 mL min-1] fractions as compared to 140 and 170 mL min-1 fractions [21.6 p.c. (5.2) and 31.6 p.c. (15.3) for BCR-ABL FISH+ cells and 0.75 (0.16) and 0.90 (0.24) for BCR-ABL/ABL]. Fractions with the lowest proportions of BCR-ABL-positive cells and the lowest BCR-ABL/ABL transcript ratios (110 mL min-1) contained from 1.3 x 10**6 to 82.7 x 10**6 (median: 3.97 x 10**6) CD34+ cells. Conclusions: In the present study we have shown that CCE may be used effectively to obtain nLP fractions enriched in normal hematopoietic progrenitors.


    196/355

    Tytuł oryginału: Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets.
    Autorzy: Majka Marcin, Ratajczak Janina, Villaire Gaston, Kubiczek Krystyna, Marquez Leah A., Janowska-Wieczorek Anna, Ratajczak Mariusz Z.
    Źródło: Exp. Hematol. 2002: 30 (7) s.751-760, il., tab., bibliogr. 51 poz.
    Sygnatura GBL: 305,749

    Hasła klasyfikacyjne GBL:
  • hematologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: The development of megakaryocytes is regulated by thrombopoietin (TPO), which binds to the c-mpl receptor, and by several other cytokines such as interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), cilliary neurotropic factor (CNTF), and oncostatin (OSM), which bind to gp130 protein-coupled receptors. We attempted to identify signal transduction pathways activated by these factors in normal human megakaryocytes. Materials and methods: To better understand the role of these factors in normal human megakaryopoiesis we studied their effect on 1) purified human bone marrow-derived CD34+ cells, 2) human ŕIIbá3+ cells (shown by immunophenotypical and morphological criteria to be megakaryoblasts), which had been expanded ex vivo from CD34+ cells in chemically defined artificial serum, and 3) gel-filtered human peripheral blood platelets. Further, in an attempt to correlate the influence of these factors on cell proliferation and survival activation of signal transduction pathways, we evaluated their effect on the phosphorylation of MAPK p42/44 and activation of PI-3K-AKT and JAK-STAT proteins in these various cell types. Results: Using serum-free liquid cultures, we found that only TPO and IL-6 protected CD34+ cells and megakaryocytes from undergoing apoptosis (decrease in annexin-V binding, PARP cleavage, and activation of caspase-3). Moreover, only TPO when used alone and IL-6 only when used in combination with TPO, stimulated the growth of human colony-forming unitmegakarocytes (CFU-Meg) in semisolid serum-free medium...


    197/355

    Tytuł oryginału: Synthesis, DNA-binding activity and cytotoxicity of carbamate derivatives of Hoechst 33258 in breast cancer MCF-7 cells.
    Autorzy: Bielawski Krzysztof, Bielawska Anna, Wołczyński Sławomir
    Źródło: Biol. Pharm. Bull. 2002: 25 (7) s.916-919, il., tab., bibliogr. 18 poz.
    Sygnatura GBL: 313,043

    Hasła klasyfikacyjne GBL:
  • farmacja
  • ginekologia i położnictwo
  • onkologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: A series of carbamate derivatives of Hoechst 33258 was prepared as potential anticancer agents. These new compounds (1-4) were readily prepared in good yields by addition of chloroethyl, bromoethyl, chloropropyl or 4-(chloromethyl)phenyl isocyanates to Hoechst 33258. Their cytotoxic activity was evaluated on human breast cancer MCF-7. Compounds 1-4 were more cytotoxic than Hoechst 33258. In particular derivatives 4, the most active of the series, is up to 3 times more potent than Hoechst 33258. The DNA-binding ability of these compounds was evaluated by an ultrafiltration method using calf thymus DNA. These data show that in broad terms the cytotoxic potency of 1-4 in cultured breast cancer MCF-7 cells incerases, in accord with their increases in DNA affinity, as shown by the binding constant values.


    198/355

    Tytuł oryginału: The effect of ascorbic acid and ethanol on the level of thiobarbituric acid reactive substances (TBARS) in selected tissues of albino BALB/c mice.
    Autorzy: Woźniak Alina, Woźniak Bartosz, Drewa Gerard, Kasprzak Heliodor Adam
    Źródło: Biol. Pharm. Bull. 2002: 25 (7) s.943-944, tab., bibliogr. 14 poz.
    Sygnatura GBL: 313,043

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: The aim of the paper was to study the effect of ascorbic acid (vitamin C) and ethanol on the level of thiobarbituric acid reactive substances (TBARS) in the brain, lungs and blood serum of inbred albino BALB/c mice. Sixty male mice divided into 4 groups were used for the study; the mice in each group were injected intraperitoneally (.p.): Group 1 - control, saline; Group 2 - H202 and FeSO4; Group 3 - ascorbic acid (AA); Group 4 - AA and ethanol. The level of TBARS in the investigated tissues of the mice in all of the groups was statistically significant higher than than in the control group. The highest content of TBARS in blood serum, brain, liver and lung tissue was observed in mice which were given ascorbic acid and ethanol.


    199/355

    Tytuł oryginału: Impaired DNA damage-induced nuclear Rad51 foci formation uniquely characterizes Fanconi anemia group D1.
    Autorzy: Godthelp Barbara C., Artwert Fr‚, Joenje Hans, Zdzienicka Małgorzta Z.
    Źródło: Oncogene 2002: 21 (32) s.5002-5005, il., tab., bibliogr. [29] poz.
    Sygnatura GBL: 312,857

    Hasła klasyfikacyjne GBL:
  • hematologia

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Fanconi anemia is a hereditary cancer susceptibility disorder chracterized at the cellular level by spontaneous chromosomal instability and specific hypersensitivity to DNA cross-linking agents such as mitomycin C. This phenotype suggests a possible role for the Fanconi anemia proteins in the repair of DNA lesions induced by these agents, but the molecular mechanism underlying the defect in this disorder has not yet been identified. Here, we show that amongst eight so far identified complementation groups of Fanconi anemia, only fibroblasts derived from group D1 are defective in the formation of nuclear Rad51 foci after X-ray irradiation or mitomycin C treatment. This indicates that the FANCD1 gene product is uniquely involved in the assembly and/or stabilization of the Rad51 complex. Since DNA dmage-induced Rad51 nuclear foci are thought to reflect of DNA double-strand breaks by homologous recombination, our results suggest that FANCD1 is likely to be involved in homologous recombination-dependent repair.


    200/355

    Tytuł oryginału: Endothelium-dependent production and liberation of kynurenic acid by rat aortic rings exposed to L-kynurenine.
    Autorzy: Stążka Janusz, Luchowski Piotr, Wielosz Marian, Kleinrok Zdzisław, Urbańska Ewa M.
    Źródło: Eur. J. Pharmacol. 2002: 448 (2/3) s.133-137, il., bibliogr. [19] poz.
    Sygnatura GBL: 312,088

    Hasła klasyfikacyjne GBL:
  • farmacja
  • kardiologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • in vitro

    Streszczenie angielskie: Rat aortic slices produced and liberated the endogenous antagonist of glutamate receptors, kynurenic acid, upon exposure to L-kynurenine. Endothelium-denuded slices did not synthesize any measurable amount of kynurenic acid, indicating its endothelial origin. Aortic kynurenic acid production was diminished by modification of the ionic milieu, hypoxia and hypoglycemia, as well as by L-glutamate and L-aspartate, endogenous glutamate receptor agonists, and aminooxyacetic acid, a non-selective inhibitor of aminotransferase and mitochondrial respiration. These data pave the way for future research aimed to clarify to the role of kynurenic acid in the physiology and pathology of the endothelium and vasculature.


    201/355

    Tytuł oryginału: Low-dose ritonavir moderately enhances nelfinavir exposure.
    Autorzy: Kurowski Michael, Kaeser Benoite, Sawyer Anthony, Popescu Matei, Mrozikiewicz Alexander
    Źródło: Clin. Pharmacol. Ther. 2002: 72 (2) s.123-132, il., tab., bibliogr. 37 poz.
    Sygnatura GBL: 311,916

    Hasła klasyfikacyjne GBL:
  • aIDS
  • farmacja
  • choroby zakaźne

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.

    Streszczenie angielskie: Bacground: The protease inhibitor ritonavir is increasingly administered at subtherapeutic doses in highly acyive antiretroviral treatment, to utilize its potential for drug interactions and to enhance the plasma concentrations of other concomitantly prescribed protease inhibitors. The addition of low doses of ritonavir to nelfinvir as investigated to describe the extent of pharmacokinetic interaction. Methods: In this randomized, open-label, one-sequence crossover study, nelfinavir 1250 mg a day was dosed for 17 days, followed by 14 days of nelfinvar 1250 mg twice a day plus low doses of ritonavir of either 100 mg or 200 mg orally. Twenty-four healthy volunteers were evaluated for pharmacokinetics of nelfinavir, its metabolite M8, and ritonavir. Plasma concentrations were measured up 12 hours after moring and evening dosing, respectively, on days 14 and 31. Results: Ritonavir increased the area under the plasma concentration-time curve (AUC) of nelfinvir by 20 p.c. (P = .024) and 39 p.c. (P = .001) after moring and evening administration, respectively. The AUC of nelfinavir metabolite M8 was increased by 74 p.c. and 86 p.c. after moring and evening dosing (P .001 for both). Conclusion: During ritonavir combination therapy a clear although minor drug effect on nelfinavir pharmacokinetics was demonstrated but no dose effect was shown.


    202/355

    Tytuł oryginału: Jan Mikulicz-Radecki (1850-1905) - the creator of modern European surgery.
    Autorzy: Gorecki Piotr, Gorecki Wojciech
    Źródło: Dig. Surg. 2002: 19 (4) s.313-318, il., bibliogr. 26 poz.
    Sygnatura GBL: 313,013

    Hasła klasyfikacyjne GBL:
  • chirurgia

    Typ dokumentu:
  • praca biograficzna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Temat osobowy:
  • Mikulicz-Radecki
  • Jan 1850-1905


    203/355

    Tytuł oryginału: Atherosclerosis
    Tytuł angielski: Inverse relationship between total testosterone and anti-oxidized low density lipoprotein antibody levels in ageing males.
    Autorzy: Barud Wojciech, Palusiński Robert, Bełtowski Jerzy, Wójcicka Grażyna
    Źródło: Atherosclerosis 2002: 164 (2) s.283-288, il., tab., bibliogr. 49 poz.
    Sygnatura GBL: 304,815

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • płeć męska

    Streszczenie angielskie: Accumulating evidence indicates the involvement of sex hormones in atherogenesis. Endogenous testosterone is inversely related to the majority of risk factors for atherosclerosis and is known to be a potent immunomodulator. Recently, autoantibodies to oxidized LDL (anti-oxLDL Ab) were shown to predict carotid and coronary atherosclerosis. The aim of this study was to investigate the relationship between these antibodies and testosterone level in ageing males. The study group comprised 65 males over 50 years old (42 with coronary artery disease). Serum anti-oxLDL Ab titer was measured by enzyme-linked immunoassay and total serum testosterone by radioimmunoassay. A significant inverse correlation was found between serum anti-oxLDL Ab titer and testosterone concentration (r = -0.346, P = 0.0047). Alteration in serum anti-oxLDL Ab titers showed no correlation to classical cardiovascular risk factors, e.g. body mass index, waist/hip ratio, smoking, total cholesterol, triglicerides, HDL-cholesterol, LDL-cholesterol. In multiple regression analysis only testosterone level was independently associated with anti-oxLDL Ab. These data suggest that a fall of testosterone concentration in ageing men can influence either oxidative modification of LDL or the immune response to these lipoproteins which may be important in the pathogenesis of atherosclerosis.


    204/355

    Tytuł oryginału: Azapeptides structurally based upon inhibitory sites of cystatins as potent and selective inhibitors of cysteine proteases.
    Autorzy: Wieczerzak Ewa, Drabik Piotr, Łankiewicz Leszek, Ołdziej Stanisław, Grzonka Zbigniew, Abrahamson Magnus, Grubb Anders, Br”mme Dieter
    Źródło: J. Med. Chem. 2002: 45 (19) s.4202-4211, il., tab., bibliogr. 69 poz.
    Sygnatura GBL: 312,133

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: A series of azapeptides as potential inhibitors of cysteine protease were synthesized. Their structures, based on the binding center of cystatins, contain an azaglycine residue (Agly) in place of the evolutionary conserved glycine residue in the N-terminal part of the enzyme binding region of cystatins. Incorporation of Agly should lead to deactivation of the acyl-enzyme complex formed against nucleophilic attack by water molecules in the final step of peptide bond hydrolysis. The majority of synthesized azapeptides shows high inhibitory potency toward the investigated cysteine proteases, papain, cathepsin B, and cathepsin K. One of them, Z-Arg-Leu-Val-Agly-Ile-Val-OMe (compound 17), which contains in its sequence the amino acid residues from the N-terminal binding segment as well as the hydrophobic residues from the first binding loop of human cystain C, proved to be a highly potent an selective inhibitor of cathepsin B. It inhibits cathepsin B with a Ki value of 0.088 nM. To investigate the influence of the structure of compound 17 for its inhibitory properties, we determined its conformation by means of NMR studies and theoretical calculations. The Z-Arg-Leu-Val-Agly fragment covalenty linked to Cys29 of cathepsin B, was also developed and modeled, in the catalytic pocket of the enzyme, through a molecular dynamics approach, to analyze ligand - protein interactions in detail. Analysis of the simulation trajectories generated using the AMBER force field provided us with atomic-level understanding of the conformational variability of this inhibitor, which is discussed in the context of other experimental and theoretical data.


    205/355

    Tytuł oryginału: Global motion detection is impaired in cats deprived early of pattern vision.
    Autorzy: Burnat Kalina, Vandenbussche Erik, Żernicki Bogusław
    Źródło: Behav. Brain Res. 2002: 134 (1/2) s.59-65, il., bibliogr. 45 poz.
    Sygnatura GBL: 312,662

    Hasła klasyfikacyjne GBL:
  • neurologia
  • okulistyka

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • koty

    Streszczenie angielskie: We investigated global motion detection in binocularly deprived cats (BD cats) and control cats (C cats). The cats were trained in the two-choice free running apparatus for a food reward. The positive stimulus was a moving random-dot pattern with all dots moving in one direction, the negative stimulus was the same random-dot pattern but stationary. The BD cars were severly impaired in detection of global motion stimulus as compared with the C cats. In contrast, their level of performance in a simple relative motion detection task (one square) did not differ from that in the C cats. However, in more complex relative motion detection task (two squares) the performance of the BD cats was impaired. The deficit in the detection of global motion in BD cats may be due to impairments of their Y-pathway.


    206/355

    Tytuł oryginału: Septal vasopressin induced preservation of social recognition in rats was abolished by pinealectomy.
    Autorzy: Appenrodt Edgar, Juszczak Marlena, Schwarzberg Helmut
    Źródło: Behav. Brain Res. 2002: 134 (1/2) s.67-73, il., tab., bibliogr. 47 poz.
    Sygnatura GBL: 312,662

    Hasła klasyfikacyjne GBL:
  • neurologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The role of intraseptal vasopressin (AVP) and the pineal gland in the modulation of social memory was investigated. For social recognition, male pinealectomised (Px) and sham-operated (SO) rats were confronted with juveniles for 4 min, and injected with either 200 pg AVP or 5 ng of its V1 receptor antagonist d(CH2)5Tyr(Me)AVP(AAVP) into the mediolateral septum. Re-exposure to the same and a different juvenile took place after 30 or 120 min. In SO rats, the social memory was facilitated after injection of AVP (120 min) and impaired after AAV application (30 min). In Px rats, however, neither AVP nor AAVP administration influenced the social memory. Comparison between SO and Px control groups treated with artificial cerebrospinal fluid did not reveal any differences as to social recognition responses. After subcutaneous administration of 250 ćg melatonin (substitution for pinealectomy) the social discrimination responses in Px rats after peptide application were influenced in a manner identical to that noted in SO animals. These finding suggest that the modulation of social memory by intraseptal AVP is dependent on an intact pineal function.


    207/355

    Tytuł oryginału: Protein Z in young survivors of ischemic stroke.
    Autorzy: Łopaciuk Stanisław, Bykowska Ksenia, Kwieciński Hubert, Członkowska Anna, Kuczyńska-Zardzewiały Arletta
    Źródło: Thromb. Haemost. 2002: 88 (3) s.536, il., bibliogr. 5 poz.
    Sygnatura GBL: 304,333

    Hasła klasyfikacyjne GBL:
  • pediatria
  • neurologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • list i notatka
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska


    208/355

    Tytuł oryginału: Fibrous-long spacing collagen in post-partum rat cervix uteri.
    Autorzy: Mackiewicz Zygmunt, Stepaniuk Mirosława, Mackevicius Zigmuntas jr
    Źródło: Acta Med. Lituan. 2002: 9 (2) s.126-129, il., bibliogr. 20 poz., santr.
    Sygnatura GBL: 313,255

    Hasła klasyfikacyjne GBL:
  • ginekologia i położnictwo

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć żeńska

    Streszczenie angielskie: Fibrous-long spacings (FLS) are one of atypical or artificial forms of collagen fibrils. We analyzed occurrence of this form of interstitial collagens of cervix uteri in pregnant, nonpregnant and post-partum white Wistar rats. Light and electron microscopy were used. Extensive collagenolysis in rat cervical stroma on the eve of labour and in close post-partum period was found. Extravasated activated eosinophils participated in this process. In the solid milieu of massive collagenolysis FLS were found. FLS were not found in nonpregnant cervix ureri. Administration of bleomycin had no influence on collagen degradation rate or on FLS formation.


    209/355

    Tytuł oryginału: Synthesis, antiarrhythmic, and antihypertensive effect of novel 1-substituted pyrrolidin-2-one and pyrrolidine derivatives with adrenolytic activity.
    Autorzy: Malawska Barbara, Kulig Katarzyna, Filipek Barbara, Sapa Jacek, Maciąg Dorota, Zygmunt Małgorzata, Antkiewicz-Michaluk Lucyna
    Źródło: Eur. J. Med. Chem. 2002: 37 (3) s.183-195, il., tab., bibliogr. 23 poz.
    Sygnatura GBL: 312,629

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: A series of 1-substituted pyrrolidin-2-one and pyrrolidine derivatives were syntheised and tested for electrocardiographic, antiarrhythmic, and antihypertensive activity as well as for ŕ1- and ŕ2-adrenoceptors binding affinities. Among the newly synthesised derivatives several compounds with 3-(4-arylpiperazin-1-yl)propyl moiety displayed strong antiarrhythmic (7a-12a) and antihypertensive (7a-11a) activities. Compound 11a, 1-[2-acetoxy-3-[4-(2-methoxyphenyl)piperazin-1-yl]propyl]pyrrolidin-2-one, was the most potent in this series. The pharmacological results and binding studies that their antiarrhythmic and hypotensive effects may be related to their ŕ-adrenolytic properties, and that those properties depend on the presence of the 1-phenylpiperazine moiety with a methoxy- or chloro- substituent in the ortho position in the phenyl ring.


    210/355

    Tytuł oryginału: The efficacy and safety of the tension-free vaginal tape procedure do not depend on he method of analgesia.
    Autorzy: Adamiak Aneta, Milart Paweł, Skorupski Paweł, Kuchnicka Katarzyna, Nestorowicz Andrzej, Jakowicki Jerzy, Rechberger Tomasz
    Źródło: Eur. Urol. 2002: 42 (1) s.29-33, il., tab., bibliogr. 17 poz.
    Sygnatura GBL: 312,572

    Hasła klasyfikacyjne GBL:
  • urologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • płeć żeńska

    Streszczenie angielskie: Objective: The oryginal tension-free vaginal tape (TVT) method, described by Ulmsten et al., routinely uses local anaesthesia during the procedure. Since the anaesthetic effect after local application of lidocaine hydrochloride was not always satisfactory we decided to introduce the spinal anaesthesia during this operation. The aim of the present study was to compare local and spinal anaesthesia with respect to their efficacy and safety in the TVT procedure. Methods: 103 women, with objectively confirmed stress urinary incontinence, were randomised into the study. Sixty-seven women were anaesthetised locally and 36 patients spinally. All TVT procedures were performed as originally described. Objective assessment of the influence of anaesthesia on intra-abdominal pressure at rest and during the cough test was done using a rectal catheter and a central venous pressure manometer. The efficacy of the TVT procedure was based on a gynaecological examination with a cough test and a three-degree subjective scale: complete cure, improvement or failure. Results: The success of the TVT procedure performed under local anaesthesia is a comparable with hat achieved under spinal analgesia (p = 0.42). The number of complications that occurred in the two groups does not differ significantly (p = 0.57). Conclusions: Spinal anaesthesia impairs the ability to cough effectively during the TVT procedure. However, the efficacy and safety of the operations performed under this type of anaesthesia are comparable with the efficacy and safety of operatins done under local anaesthesia.


    211/355

    Tytuł oryginału: Cockroach allergy and exposure to cockroach allergen in Polish children with asthma.
    Autorzy: Stelmach I., Jerzyńska J., Stelmach W., Majak P., Chew G., Górski P., Kuna P.
    Źródło: Allergy 2002: 57 (8) s.701-705, il., tab., bibliogr. 25 poz.
    Sygnatura GBL: 302,302

    Hasła klasyfikacyjne GBL:
  • pediatria
  • immunologia
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Bacgriund: Asthma morbidity increases every year, especially among children, and exposure to high levels of indoor allergens is a very important factor. We evaluated the prevalence and exposure to cockroach (CR) allergen in asthmatic children in Poland, and also tested the hypothesis that asthma with allergy to CR is more severe than with allergy to other antigens. Methods: One hundred and sixty children with asthma were examined, had skin prick tests (SPT) with common and CR allergens, underwent spirometry, and provocation tests to histamine. Children with positive SPT to CR had measured specific IgE levels to this antigen and Bla g 2 concentrations were measured in their homes. Results: The most common allergen, was dust mite 51.3 p.c., followed by pollen 48.8 p.c. and CR allergen 24.3 p.c. In children with CR sensitivity, 13 p.c. had mild ashma, 26 p.c. moderate and 61 p.c. had severe asthma. Their levels forced expiratory volume in one second (FEV1), and te provocative concentration of histamine that caused a 20 p.c. fall in FEV1 (PC20), were statistically lower than in the group of children with other than CR allergies. Bla g 2 antigen was detected in 55.13 p.c. samples. The highest levels of Bla g 2 were found in old houses, without central heating, and in houses with lower income. Conclusion: In Polish children, CR allergen is a very important factor of sensitivity. Concentratins of Bla g 2 in homes are higher than previously reported in other European countries, and are strongly related to the houses' characteristics. Also, children with CR hypersensitivity have severe asthma more often than children with other allergies.


    212/355

    Tytuł oryginału: Urinary leukotriene levels are increased during exacerebation of atopic eczema/dermatitis syndrome. Relation to clinical status.
    Autorzy: Adamek-Guzik T., Guzik T. J., Czerniawska-Mysik G., Korpanty G., Mastalerz L., Radwan J., Szczeklik A.
    Źródło: Allergy 2002: 57 (8) s.732-736, il., tab., bibliogr. 24 poz.
    Sygnatura GBL: 302,302

    Hasła klasyfikacyjne GBL:
  • dermatologia i wenerologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background: Leukotrienes are potent mediators of allergic inflammation and their role in the pathogenesis of allergic disorders, particularly asthma, is well established. Their importance in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS) is still unclear. We aimed to compare urinary cysteinyl leukotriene (Cys-LT) levels during exacerbation and remission of AEDS in relation to clinical status, IgE levels, and eosinophil counts. Methods: Urinary Cys-LTs were measured by direct enzyme immunoassay in 17 adult patients with AEDS and in 17 healthy controls in whom atopy had been excluded. Cys-LTs were compared during exacerbation and remission of AEDS in relation to the clinical status measured by SCORAD. Total IgE levels were measured by enzyme-linked immunoassay (ELISA). Results: Mean clinical score during the exacerbation was 64.3 ń 3.1 and during remission 22.4 ń 4 (P 0.01). Cys-LTs were significantly higher during the exacerbation of AEDS than in the control group (230.9 ń 20.8 vs 123.2 ń 9.9 pg/mg creatinine; P 0.005). During the remission, the difference between AEDS patients and the control group was not significant (96.3 ń 8.7 vs 123.2 ń 9.9 pg/mg creatinine; P = 0.8). During AEDS exacerbation Cys-LTs levels were significantly correlated with the clinical status (rs = 0.73, P 0.01) and with eosinophil counts (r = 0.86; P 0.01) but not with the duration of the disease, age of patients, or IgE levels. Conclusions: Our results point to enhanced biosynthesis of Cys-LTs during the AEDS exacerbations. Inflammatory cells, e.g. eosinophilis are the most...


    213/355

    Tytuł oryginału: Activity of class I, II, III, and IV alcohol dehydrogenase isoenzymes in human gastric mucosa.
    Autorzy: Jelski Wojciech, Chrostek Lech, Szmitkowski Maciej, Laszewicz Wiktor
    Źródło: Dig. Dis. Sci. 2002: 47 (7) s.1554-1557, tab., bibliogr. 19 poz.
    Sygnatura GBL: 310,037

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: The gastric alcohol dehydrogenase (ADH) plays an important role in the "first pass" metabolism of ethanol. Human ADH exists in multiple forms, grouped into six classes and located in different tissues. In present study we investigated the activity of our four classes (I, II, III, and IV) of alcohol dehydrogenase isoenzymes in the different parts of stomach (corpus and antrum) in patients with suspected gastric ulcer. The aim of the study was assess the particular role of different classes of ADH in the gastric dehydrogenase actvity. For the measurement of the activity of class I and class II isoenzymes, we employed new fluorometric methods with specific substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method with n-octanol and class IV with m-nitrobenzaldehyde as a substrate, respectively. All biopsy specimens were taken from less changed areas of the antrum and body of the stomach pof 68 patients suspected of having gastric ulcer. It was found that ADH IV (gastric) activity was the highest (14.76 ń 0.68 in the corpus of the stomach in men; and 7.61 ń 0.68 in women, respectively). The activity of the ADH III isoenzyme was lower than that of ADH IV. The activities of class I and II ADH isoenzymes were barely detectable. All tested classes of ADH had higher activity in the corpus than in the antrum and in males than in females. In conclusion, the most important form of gastric ADH is isoenzyme of class IV, less important is the isoenzyme of class III. ADH classes I and II seem to have no role in the stomach.


    214/355

    Tytuł oryginału: Corticotropin-releasing hormone affects cytokine production in human HaCaT keratinocytes.
    Autorzy: Zbytek Błażej, Myśliwski Andrzej, Słomiński Andrzej, Wortsman Jacobo, Wei Edward T., Myśliwska Jolanta
    Źródło: Life Sci. 2002: 70 (9) s.1013-1021, il., bibliogr. 31 poz.
    Sygnatura GBL: 304,948

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: CRH cutaneous expression is significantly enhanced after exposure to various stimuli (Physiol Rev 2000,80;979-1020). We evaluated the effect of CRH on cytokine production in HaCaT keratinocytes, a cell line shown to express CRH receptors coupled to cAMP activation and calcium-dependent transmission pathways. It is demonstrated for the first time that exogenously added CRH stimulates production of IL-6 and IL-11. It also inhibits production of IL-1beta and does not affect TNF-alpha production. Our results indicate that CRH functioh(s) during cutaneous stress may be mediated by differential effects on cytokine production.


    215/355

    Tytuł oryginału: Indomethacin protects against neurodegeneration caused by MPTP intoxication in mice.
    Autorzy: Kurkowska-Jastrzębska I., Babiuch M., Joniec I., Przybyłkowski A., Członkowski A., Członkowska A.
    Źródło: Int. Immunopharmacol. 2002: 2 (8) s.1213-1218, il., tab., bibliogr. 12 poz.
    Sygnatura GBL: 313,577

    Hasła klasyfikacyjne GBL:
  • farmacja
  • neurologia
  • toksykologia

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • myszy

    Streszczenie angielskie: The anti-inflammatory agents are postulated to be effective in treating neurodegenerative disorders. In this study, we showed that indomethacin (IND) in the dose of 1 mg/kg protected neurons against toxic damage caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice model of Parkinson's diseases. IND also diminished microglial activation and lymphocytic infiltration in the injured areas. These observations suggest that anti-inflammatory properties of IND may play a role in the neuron's protection in this model. However, diminished inflammatory reaction may be secondary to less neuronal damage.


    216/355

    Tytuł oryginału: Concentration and composition of free ceramides in human plasma.
    Autorzy: Górska M[aria], Dobrzyń A., Żendzian-Piotrowska M., Namiot Z.
    Źródło: Horm. Metab. Res. 2002: 34 (8) s.466-468, il., tab., bibliogr. 10 poz.
    Sygnatura GBL: 312,226

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie


    217/355

    Tytuł oryginału: Biological properties of a new fluorescent biphalin fragment analogue.
    Autorzy: Lipkowski Andrzej W., Misicka Aleksandra, Kosson Dariusz, Kosson Piotr, Lachwa-From Magdalena, Brodzik-Bieńkowska Agnieszka, Hruby Victor J.
    Źródło: Life Sci. 2002: 70 (8) s.893-897, il., tab., bibliogr. 16 poz.
    Sygnatura GBL: 304,948

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Previous studies of stsurcture-activity of biphalin defined fragments which expressed the full biological potency of the parent compound. The most simple fragment was Tyr-D-Ala-Gly-Phe-NH-HH - X, where X = Phe, but it also could be other hydrophobic amino acids. This paper presents data that replacement of the phenylalanine with a dansyl (X = DNA) groups gives an analogue (AA20116) that fully preserves the high affinity of the initial analogue for both ć and ë opiioid receptors. In the tail flick test in rats, intrathecal injection of the compound produces strong antinociception, comparable to the parent biphalin. Cecause AA2016 contains a strong fluorescent group, it can be a very useful tool for prospective studies in vivo, including biological barrier permeability, tissue distribution, metabolism and receptor-ligand complex formation.


    218/355

    Tytuł oryginału: Superficialization of the wrist native arteriovenous fistula for effective hemodialysis vascular access construction.
    Autorzy: Weyde Wacław, Krajewska Magdalena, Letachowicz Waldemar, Klinger Marian
    Źródło: Kidney Int. 2002: 61 (3) s.1170-1173, il., bibliogr. 10 poz.
    Sygnatura GBL: 312,423

    Hasła klasyfikacyjne GBL:
  • nefrologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dzieci 13-18 r.ż.
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background. It is commonly known that the best type of vascular access for chronic hemodialysis, defined as the one with the lowest number of complications and longest use, is the primary (Brescia-Cimino) radiocephalic fistula. One reason for the impossibility of its creation is that the vein vessel on the forearm is located too deeply, which is primarily caused by the patients' obesity. Methods. In 24 obese patients the superficialization procedure of the venous part of fistula under the skin was performed. Results. In 23 patients of this group, an easy and uncomplicated fistula puncturing was achieved. One patient had the complication of fistula clotting. Conclusion. Subcutaneous transposition of the arterialized median antebrachial vein is a simple and effective method of an adequate distal native fistula creation in patients with deeply located forearm veins.


    219/355

    Tytuł oryginału: Fibroblast-like synoviocytes from rheumatoid arthritis patients express functional IL-15 receptor complex: endogenous IL-15 in autocrine faschion enhances cell proliferation and expression of Bcl-xL and Bcl-2.
    Autorzy: Kurowska Mariola, Rudnicka Weronika, Kontny Ewa, Janicka Iwona, Chorazy Magdalena, Kowalczewski Jacek, Ziółkowska Maria, Ferrari-Lacraz Sylvie, Strom Terry B., Maśliński Włodzimierz
    Źródło: J. Immunol. 2002: 169 (4) s.1760-1767, il., bibliogr. 49 poz.
    Sygnatura GBL: 300,982

    Hasła klasyfikacyjne GBL:
  • reumatologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The hallmarks of rheumatoid arthritis (RA) are leukocytic infiltratin of the synovium and expansiveness of fibroblast-like synovicytes (FLS). The abnormal proliferation of FLS and their resistance to apoptosis is mediated, at least in part, bay present in RA joints proinflammatory cytokines and growth factors. Because IL-15 exerts properties of antiapoptotic and growth facators, and is produced by RA FLS, we hypothesized that IL-15 participates in RA FLA activation. To test this hypothesis, we first examined whether RA FLS express chains required for high affinity functional IL-15R. Indeed, RA FLS express IL-15Rŕ at mRNA and protein levels. Moreover, we confirmed the presence of IL-2Rá and common ç-chains. Interstingly, TNF-ŕ or IL-1á triggered significant elevation of IL-15Rŕ chanin at mRNA and protein levels. Next, we investigated the effects of exogenous or endogenous IL-15 on Bcl-2 andBcl-XL expression, FLS proliferation, and apoptosis. Exogenous IL-15 enhanced RA FLS proliferation and increased the level of mRNA-encoding Bcl-XL. To test the role of endogenous IL-15 in the activation of RA FLS, an IL-15 mutant/Fcç2a protein exerting properties of specific antagonist to the IL-15Rŕ chain was used. We found that blocking IL-15 biological activities using this protein substantially reduced endogenous expression of Bcl-2 and Bcl-XL, and RA FLA proliferation that was reflected by increased apoptosis. Thus, we havae demonstrated that a distinctive phenotype of RA FLS, i.e., persistent activation, proliferation, and resistance to apoptosis, is related to the autocrine activation of IL-15Rs by FLS-derived IL-15.


    220/355

    Tytuł oryginału: Disposition and metabolism of 1,6-dimethylnaphthalene in rats.
    Autorzy: Kilanowicz Anna, Sapota Andrzej, Czerski Bogdan
    Źródło: Toxicol. Lett. 2002: 134 (1/3) s.227-243, il., tab., bibliogr. [18] poz.
    Sygnatura GBL: 306,158

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: The distribution, excretion and metabolism of 1,6-dimethylnaphthalene following i.p. administration of a single dose of 20 mg/kg to rats, was investigated using radiotracer [3H] and a gas chromatography - mass spectrometry technique (GC-MS). After 72 h, about 94 p.c. of the given dose was excreted in urine and feces. In organs and tissues, the highest concentration during the first hours after administration was detected in fat, liver, spleen and kidneys. Then gradual decline of tritium was noticed in all examined tissues. In urine, the following substanceswere identified and quantified by GC peak areas: unchanged 1,6-dimethylnaphthalene, 1-methyl-hydroxynaphthalenes, 1-hydroxymethyl-6-methylnaphthalene, 1,6-dimenthyl-thionaphthalene, 6-methyl-1-naphthoic aldehyde, 6-methyl-1-naphthoic acid, 1,6-dimethyl-thionaphthalene and 1,6-dimethyl-methylthionaphthalene.


    221/355

    Tytuł oryginału: Acute and subacute nephrotoxicity of 2-bromophenol in rats.
    Autorzy: Bruchajzer Elżbieta, Szymańska Jadwiga A., Piotrowski Jerzy K.
    Źródło: Toxicol. Lett. 2002: 134 (1/3) s.245-252, il., tab., bibliogr. [24] poz.
    Sygnatura GBL: 306,158

    Hasła klasyfikacyjne GBL:
  • toksykologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury
  • płeć żeńska

    Streszczenie angielskie: The present report aims at providing broader information on the acute nephrotoxicity of 2-bromophenol (2-BP) (a bromobenzene (BB) metabolite), due to its action on the kidneys, after repeated administration. Investigations were performed on female rats. Following a single dose, the most pronounced changes involved: concentrations and rates of excretion of proteins in urinwe, the number of epithelial cells excreted in urine, creatinine and urea clearance and reduced gluthione in renal tissue. Immediate effects could be ascribed to both renal tubules and glomeruli, mirrored in the level of urinary proteins and intensified excretion of renal epithelial cells. Less pronounced changes of the indicator values were noted under repeated dosing of 2-BP. The results obtained in a single exposure study confirm earlier on the mild nephrotoxicity of 2-BP following exposure to high doses. However, the transition from single to repeated expopsure does not result in enhanced nephrotoxicity.


    222/355

    Tytuł oryginału: Relationship between hyperinsulinemia and angiographically defined coronary atherosclerosis in non-diabetic men.
    Autorzy: Kruszelnicka-Kwiatkowska O., Surdacki A., Goldsztajn P., Matysek J., Piwowarska W., Golay A.
    Źródło: Diabetes Metab. 2002: 28 (4 c. 1) s.305-309, tab., bibliogr. 24 poz.
    Sygnatura GBL: 313,123

    Hasła klasyfikacyjne GBL:
  • endokrynologia
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • płeć męska

    Streszczenie angielskie: Background. Our aim was estimate the relationship between hyperinsulinemia and angiographically validated coronary atherosclerosis. Methods: 102 consecutive non-diabetic male subjects (age 48.9 ń 9.6 years) with a body mass index 25.9 ń 2.8 kg/mý referred to coronary angiography in our centre were studied. A 75-g oral glucose tolerance test (OGTT) was performed and the areas under the curve (AUC) of serum insulin and glucose were calculated. Results: Discriminant analysis with stepwise forward variable selection revealed that in younger patients ( 50 years), the following parameters affected positively the number of significant coronary lumen reductions: age (F = 8.5, p = 0.005). InAUCinsulin (F = 5.8, p = 0.02), low HDL cholesterol (F = 4.4, p =n 0.04), the smoking habit (F = 4.1, p = 0.05). In those 50 years of age, exclusively age (F = 5.8, p = 0.02) and hyperuricemia (F = 3.8, p = 0.06) entered of the final model. Conclusion: Our results indicate that in non-diabetic male subjects the association of hyperinsulinemia with the severity of coronary arterosclerosis is only seen in younger patients.


    223/355

    Tytuł oryginału: In situ activation of caspases and serine proteases during apoptosis detected by affinity labeling their enzyme active centers with fluorochrome-tagged inhibitors.
    Autorzy: Grabarek Jerzy, Darzynkiewicz Zbigniew
    Źródło: Exp. Hematol. 2002: 30 (9) s.982-989, il., bibliogr. 60 poz.
    Sygnatura GBL: 305,749

    Typ dokumentu:
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Streszczenie angielskie: Activation of caspases is the key event of apoptosis. To detect this event in situ we applied fluorochrome-labeled inhibitors of caspases (FLICA) as affinity of activity centers of these enzymes. The FLICA are fluoroscein- or sulforhodamine-conjugated peptide-fluoromethyl ketones that covalently, with 1:1 stoichiometry, bind to enzymatic centers of caspases; the specifity is provided by the peptide sequence of amino acids. Similarly, we applied fluorescent inhibitors of serine proteases (FLISP) to detected activite sites of the latter enzymes. Exposure of live cells to FLICA of FLISP led to uptake of these ligands and their binding to activated caspases or active sites og serine proteases; the unbound reagents were removed by cell rinse. Only cells undergoing apoptosis were labeled with FLISP or FLICA. Intracellular binding sites of FLICA are consistent with known localization of caspases. Covalent binding of FLICA or FLIPPS allowed us to identify the labeled proteins by immunoblotting: the proteins that bound individual FLICAs had molecular weight between 17 and 22 kDa, which corresponds to large subunits of the caspases; two proteins reacting with FLISP were about 57 and 60 kDa, which suggests that they are novel enzymes. Detection of caspases or serine proteases activation can be combined with other markers of apoptosis or cell cycle for multiparametric analysis by flow or laser ascanning cytometry. Being caspases inhibitors, FLICA arrest the process of apoptosis and prevent cell distintegration. The stathmo-apoptotic assay was developed...


    224/355

    Tytuł oryginału: Integrated minimally invasive direct coronary artery bypass grafting and angioplasty for coronary artery revascularization.
    Autorzy: Cisowski Marek, Morawski Włodzimierz, Drzewiecki Janusz, Kruczak Wojciech, Toczek Krzysztof, Bis Jarosław, Bochenek Andrzej
    Źródło: Eur. J. Cardiothorac. Surg. 2002: 22 (2) s.261-265, tab., bibliogr. 20 poz.
    Sygnatura GBL: 312,899

    Hasła klasyfikacyjne GBL:
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Objective: Minimally invasive direct coronary artery bypass (MIDCAB) through the anterolateral minithoractomy has become a promising therapeutic option in patients with lesion in left anterior descending artery (LAD), especially in multimorbid, elderly and reoperated patients with type C or B lesions. To expand the benefits of MIDCAB concept to patients with multivessel disease, a hybrid myocardial revascularization procedure (HMR) combining surgery of the LAD with interventional proedures for additional coronary lesions has recently been introduced. Methods: Between January 1999 and September 2001, 50 patients (37 male, 13 female, mean age 54.8 ń 20.1 years) underwent an HMR procedure. MIDCAB with endoscopic left internal thoracic artery (LITA) harvesting, followed by percutaneous coronary intervention (PCI) for additional coronary lesions and percutaneous transluminal coronary angioplasty (PTCA), was performed in 11 patients (22 p.c.) and stenting in 39 patients (78 p.c.). Angiographic assessment of graft patency was performed in all patients during the PCI procedure. The clinical follow-up period was 3-32 months. Results: There were no early and late deaths. Baseline Canadian Cardiology Society (CCS) class was 2.8 ń 0.7 versus 1.1 ń 0.9 (P 0.001) 30 days after HMR procedure. There were no major acute inhospital cardiac events. Angiographic studies showed patent LIMA-LAD graft in 50 patients (100 p.c.). We showed quality of anastomosis in 49 patients (98 p.c.). There was a moderate graft stenosis in one ...


    225/355

    Tytuł oryginału: Soluble adhesion molecules (ICAM-1, VCAM-1, and E-selectin) and vascular endothelial growth factor (VEGF) in patients with distinct variants of rheumatoid synovitis.
    Autorzy: Klimiuk P. A., Sierakowski S., Latosiewicz R., Cylwik J. P., Cylwik B., Skowroński J., Chwiecko J.
    Źródło: Ann. Rheum. Dis. 2002: 61 (9) s.804-809, il., tab., bibliogr. 46 poz.
    Sygnatura GBL: 310,264

    Hasła klasyfikacyjne GBL:
  • reumatologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Background: Cell adhesion molecules and endothelial growth factors have an important role in the infiltrating of rheumatoid synovium with mononuclear cells, leading to the initiation and progression of the disease. Objective: To investigate whether the serum profile of soluble adhesion molecules and of vascular endothelial growth factor (VEGF) is associated with the histological apperance of rheumatoid arthritis (RA). Methods; Serum levels of soluble intercellular adhesion molecule-1 (slCAM-1), vascular cell adhesion molecule-1 (sVCAM-1), E-selectin (sE-selectin), and VEGF were assessed by enzyme linked immunosorbent assay (ELISA) in 40 patients with RA and 32 patients with osteoarthritis (OA). Results: Histological analysis of synovium specimens distinguished two types of rheumatoid synovitis. Twenty four RA samples presented diffuse infiltrates of mononuclear cells without any further microanatomical organisation, whereas in the remaining 16 samples lymphocytic follicles with germinal centrelike structures were identified. In comparison with patients with OA, constituting a control group, higher serum concentrations of slCAM-1 (p 0.001), sE-selectin (p,0.01), and VEGF (p0.001) were detected in patients with RA. Raised concentrations of slCAM-1, and VEGF dominated in the serum of patients with RA with follicular synovitis compared with those with diffuse synovitis (p 0.01 for all comparisons). The serum concentrations of slCAM-1, sVCAM-1, and VEGF correlated with markers of disease activity such as the erytchrocyte sedimentation rate and C reactive ...


    226/355

    Tytuł oryginału: Disturbed right ventricular ejection pattern as a new Doppler echocardiographic sign of acute pulmonary embolism.
    Autorzy: Kurzyna Marcin, Torbicki Adam, Pruszczyk Piotr, Burakowska Barbara, Fijałkowska Anna, Kober Jarosław, Oniszh Karina, Kuca Paweł, Tomkowski Witold, Burakowski Janusz, Wawrzyńska Liliana
    Źródło: Am. J. Cardiol. 2002: 90 (5) s.507-511, il., tab., bibliogr. 23 poz.
    Sygnatura GBL: 304,619

    Hasła klasyfikacyjne GBL:
  • kardiologia
  • pulmonologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 19-44 r.ż.
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Transthoracic echokardiography (TTE) is frequently performed in patients with suspected acute pulmonary embolism (APE) to search for right ventricular (RV) pressure overload. We prospectively assessed the diagnostic value on the disturbed RV ejection pattern ("60/60 sign") and compared its diagnostic performances with that of the presence of RV pressure overload, as well as with "McConnell sign" based on RV regional wall motion abnormalities. We assessed 100 consecutive patients with clinical suspicion of APE, including those with previous cardiorespiratory disease. After TTE, all of the patients underwent reference diagnostic tests for APE. The 60/60 sign required RV occeleration time of ó 60 ms in presence of tricuspid insufficiency pressure gradient ó 60 mm Hg. APE was ultimately confirmed in 67 of 100 patients. In 17 of 67 patients, 60/60 sign correctly suggested APE, whereas 2 false-positive results were noted: in pulmonary fibrosis and in acute respiratory distress syndrome following lung resection. McConnell sign was fully specific but was noted in only 13 of 67 patients. Sensitivity and specificity were 81 p.c. and 45 p.c. for "RV-pressure overload" signs, 25 p.c. and 94 p.c. for 60/60 sign, and 19 p.c. and 100 p.c. for McConnell sign. When combined, the 2 latter signs were 94 p.c. specific and 36 p.c. sensitive in diagnosing APE. Thus, RV overload at echocardiography is not specific for APE. The 60/60 and McConnell signs are insensitive, but are reliabe and helpful in bedside diagnosis of APE when direct visualization of the pulmonary arteries is impossible...


    227/355

    Tytuł oryginału: Intravascular ultrasonic evaluation of the magnitude of stent expansion and the mechanisms of lumen enlargement after direct stenting and after conventional stenting with balloon predilatation.
    Autorzy: Dudek Dariusz, Legutko Jacek, Kałuża Grzegorz L., Żmudka Krzysztof, Dubiel Jacek S.
    Źródło: Am. J. Cardiol. 2002: 90 (6) s.639-641, il., tab., bibliogr. 9 poz.
    Sygnatura GBL: 304,619

    Hasła klasyfikacyjne GBL:
  • kardiologia
  • radiologia

    Typ dokumentu:
  • praca kliniczna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • dorośli 45-64 r.ż.
  • dorośli = 65 r.ż.
  • płeć męska
  • płeć żeńska

    Streszczenie angielskie: Currently, a growing number of stents are deployed in a coronary artery without, a preceding ballon dilation (direct stenting [DS]). The mechanisms of lumen enlargement after conventional stent implantation have been recently studied by intravascular ultrasound (IVUS) and encompass: (1) vessel expansion, (2) axial redistribution of plaque, and (3) plaque compression or embolization. In contrast, the impact of direct stent implantation on the plaque and vessel segment undergoing intervention has not been charcterized. The present study investigates the mechanisms of lumen enlargement after coronary DS.


    228/355

    Tytuł oryginału: Tissue-specific association of the human tyrosine hydroxylase gene with the nuclear matrix.
    Autorzy: Lenartowski Robert, Goc Anna
    Źródło: Neurosci. Lett. 2002: 330 (2) s.151-154, il., bibliogr. 20 poz.
    Sygnatura GBL: 305,936

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą