Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

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Tytuł oryginału: Angiogenesis inhibitors specific for methionine aminopeptidase 2 as drugs for malaria and Leishmaniasis.
Autorzy: Zhang Peng, Nicholson Diarmuid E., Bujnicki Janusz M., Su Xinzhuan, Brendle James J., Ferdig Michael, Kyle Dennis E., Milhous Wilbur K., Chiang Peter K.
Źródło: J. Biomed. Sci. 2002: 9 (1) s.34-40, il., tab., bibliogr. 27 poz.
Sygnatura GBL: 313,488

Hasła klasyfikacyjne GBL:
  • mikrobiologia
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • praca opublikowana za granicą
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • in vitro

    Streszczenie angielskie: Methionine aminopeptidase 2(MetAP2) is responsible for the hydrolysis of the initiator methionine molecule from the majority of newly synthesized proteins. We have cloned the MetAP2 gene from the malaria parasite Plasmodium falciparum (PfMetAP2; GenBank accession number AF348320). The cloned PfMetAP2 has no intron, consists of 1,544 bp and encodes a protein of 354 amino acids with a molecular mass of 40,537 D and an overall base composition of 72.54 p.c. A + T. PfMetAP2 has 40 p.c. sequence identity with human MetAP2 and 45 p.c. identity with yeast MetAP2, and is located in chromosome 14 of P. falciparum. The three-dimensional structure of PfMetAP2 has been modeled based on the crystal structure of human MetAP2, and several amino acid side chains protruding into the binding pocket that differ between the plasmodial and human enzyme have been identified. The specific MetAP2 inhibitors, fumagilin and TNP-470, potently blocked in vitro growth of P. falciparum and Leishmania donovani, with IC50 values similar to the prototype drugs. Furthermore, in the case of P. falciparum, the chloroquine-resistant strains are equally susceptible to these two compounds.

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    Tytuł oryginału: Hepatic cirrhosis increases sensitivity of kidney to endotoxin in rats.
    Autorzy: Liu Jian-Jun, Wang Ji-Yao, Zhang Chi, Nilsson Ake, Duan Rui-Dong
    Źródło: Med. Sci. Monitor 2002: 8 (2) s.BR56-BR60, il., bibliogr. 31 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • gastroenterologia
  • nefrologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • szczury

    Streszczenie angielskie: Background: Renal failure in cirrhotic patients is a severe complication and endotoxemia might be involved. We investigated the effect of endotoxin on renal function of cirrhotic rats and the potential protective role of N-acetylcysteine (NAC). Material/Methods: Hepatic cirrhosis was generated in a rat model by carbon tetrachloride. Both cirrhotic and normal rats were insulted by endotoxin intravenously, while another cirrhotic group was pretreated with NAC. Blood urea nitrogen (BUN) and creatinine were assayed eight hours later. The changes in serum tumor necrosis factor-ŕ (TNF-ŕ) were assayed by ELISA. The histological changes in the kidney were observed after hematoxylinand eosin staining. Results: Endotoxin increased the BUN and creatinine levels in both normal and cirrhotic rats, with a much higher elevation in the latter group. TNF-ŕ concentration was also increase by endotoxin; the changes are positively correlated with BUN and creatinine. NAC pretreatment sigificantly attenuates the effects of endotoxin on BUN, creatnine and TNF-ŕ levels in cirrhotic rats with no improvement in systemic toxicity symptoms. There were no obvious histological changes in the kidney of these animals. Conclusion: Hepatic cirrhosis increased the sensitivity of renal function to endotoxemia, which may be protected by NAC.

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    Tytuł oryginału: Diminished expression of the type II receptor for TGFá (TGFáRII) in T lymphocytes from patients with sezary syndrome is not due to mutations in the receptor's poly-A tract: limitations of the standard RT-PCR in cDNA sequence analysis of homopolymeric base stretches.
    Autorzy: Zhang Qian, Capocasale Renold J., Fox Floyd E., Bedian Vahe, Vonderheid Eric C., Rook Alain, Moore Jonni S., Nowell Peter C., Haines Dale S., Wasik Mariusz A.
    Źródło: Arch. Immunol. Ther. Exp. 2002: 50 (6) s.421-429, il., tab., bibliogr. 26 poz.
    Sygnatura GBL: 304,223

    Hasła klasyfikacyjne GBL:
  • genetyka
  • hematologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Perihperal blood lymphocytes from patients with Sezary syndrome (SzS) frequently demonstrate decreased surface expression of transforming growth factor á receptor II (TGFáRII). The mechanism of this low TGFáRII expression remains unknown. Because mutations within the poly-A tract of the TGFáRII sequence (nucleotides 709 - 718) were shown to result in diminished TGFáRII expression in other types of malignant tumors, we examined the sequence of the TGFáRII poly-A tract in two SzS-derived cell lines and in peripheral blood SzS cells from 17 SzS patients and 4 control, healthy individuals using DNA sequencing and single-stranded conformation polymorphism (SSCP) analysis. A standard bidirectional, automated sequence analysis of the RT-PCR-generated cDNA TGFáRII fragment showed a heterogenous population of the normal length, 10-, with admixed, shortened, 9-base poly-A stretches. Surprisingly, this mixture was present not only in the cells from 5 SzS patients and 2 SzS cell lines, but also in cells from 2 healthy control individuals. Importantly, the proportion of the shortened, 9-base fragments was markedly reduced or practically eliminated when the procedure was modified by usage of high-fidelity DNA polymerase, labeled primers and/or cloned RT-PCR products, which indicates that the presence of the shortened, 9-base fragments represented a procedural phenomenon rather than a true deletional mutation within an allele of the TGFáRII gene. Accordingly, SSCP analysis of genomic DNA did not reveal any mutations within the poly-A tract-containing region. These results indicate ...

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    Tytuł oryginału: The influence of sex hormones on circulating nitric oxide (NOx) levels in Rhesus monkeys (Macaca Mulatta).
    Autorzy: Khorram Omid, Colman Ricki J., Kemnitz Joseph W., Magness Ronald R., Zhang Jian, Yao Zhi, Keller Evan T.
    Źródło: Med. Sci. Monitor 2002: 8 (12) s.BR489-BR495, il., tab., bibliogr. 36 poz.
    Sygnatura GBL: 313,278

    Hasła klasyfikacyjne GBL:
  • endokrynologia

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • zwierzęta
  • małpy
  • płeć żeńska

    Streszczenie angielskie: Background: The effects of long-term estrogen (E2) replacement therapy (ERT) on circulating and bone marrow total plasma nitric oxide (NOx) levels were determined in rhesus monkeys. In addition changes in circulating NOx during the menstrual cycle were measured. We measured Nox levels in 20 ovariectomized animals and 27 cycling animals. In the first group monkeys were ovariectomized (OVX; n=10) and treated with estrogen implants for 21 months. The control group (n=10) was OVX and received vehicle implants only. After 21 months systemic NOx status was measured. In a second group of cycling animals NOx was measured during the follicular and luteal phases, and correlated whit body comparison parameters determined by dual energy X-ray absorptiometry (DXA) analysis. Results: Replacement of estrogen in OVX monkeys resulted in reduced circulating but not bone marrow plasma NOx compared to OVX monkeys. Circulating NOx levels in these animals correlated negatively (rý= -0.35; P=0.03) with estradiol levels. In cycling monkeys luteal levels of NOx were found to be significantly higher than follicular levels and correlated negatively (rý=-0.4; P=0.036) with estradiol but not progesterone levels. Negative correlations between NOx levels and body weight, fat and lean mass were also found. Conclusion: Long-term ERT influences systemic NOx levels in negative fashion without effects on bone marrow plasma NOx. Circulating NOx was higher during the luteal phase. In both ERT treated and cycling monkeys estradiol co\related negatively with NOx suggesting the existence of a negative...

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