Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: SZEMRAJ
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Tytuł oryginału: The isoform- and location-dependence of the functioning of the plasma membrane calcium pump.
Autorzy: Żylińska Ludmiła, Kawecka Iwona, Lachowicz Lilla, Szemraj Janusz
Źródło: Cell. Mol. Biol. Lett. 2002: 7 (4) s.1037-1045, il., tab., bibliogr. 26 poz.
Sygnatura GBL: 306,513

Typ dokumentu:
  • tytuł obcojęzyczny

    Streszczenie angielskie: The plasma membrane is a specialised multi-component structure with inter- and intracellular signalling functions. Ca**2+ plays a crucial role in cellular physiology, and an ATP-driven plasma membrane calcium pump (PMCA) plays the greatest role in the maintenance of a low free Ca**2+ concentration in the cytoplasm. The enzyme is coded by four separate genes (PMCA 1-4), and, due to alternative splicing, more than 20 variants can exist. PMCA 1 and 4 isoforms are present in almost all tissues, whereas PMCA 2 and 3 are found in more specialised cell types. The variants differ primarily in their regulatory regions, thus the modulation of calcium pump activity strongly depends on teh isoform and the membrane omposition. The unique function of PMCA isoforms was confirmed using the practical experimental models - a rat pheochromocytoma cell line, a human neuroblastoma cell line, or, more recently knockout mice. In addition, based on the finding that PMCA could interact with several specific signaling proteins, it was concluded that its location in defined sites of the cell membrane could be a prerequisite for efficient intercellular communication.

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    Tytuł oryginału: Reactive oxygen species upregulate expression of PAI-1 in endothelial cells.
    Autorzy: Świątkowska Maria, Szemraj Janusz, Al-Nedawi Khalid N.I., Pawłowska Zofia
    Źródło: Cell. Mol. Biol. Lett. 2002: 7 (4) s.1065-1071, il., bibliogr. 18 poz.
    Sygnatura GBL: 306,513

    Typ dokumentu:
  • tytuł obcojęzyczny
  • komunikat
  • praca doświadczalna

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Second messenger involved in the signal transduction pathway leading to induction of the plasminogen activator inhibitor (PAI-1) have not yet benn wellharacterized. This study focuses on the mechanisms of regulation of PAI-1 expression by reactive oxygen species (ROS) in human endothelial cells. Inhibition of the tumor necrosis factor ŕ (TNFŕ)-induced expression of PAI-1by antioxidant N-acetyl-L-cysteine (NAC) indicated redox - sensitive mechanisms involved in the signalling pathway. Because TNFŕ induces PAI-1 production in endothelial cells, and NAC attenuated this response, we attempted to investigate the possible involvement of ROS in the activation of PAI-1 by TNFŕ. Upregulation of PAI-1 expression in endothelial cells by the stimulation with TNFŕ (50ng/ml) or H2O2 (10-200ćM), observed by measurement of the antigen and mRNA levels, was reversed in the presence of NAC (20 mM). The stimulatory effect of ROS was detected also at the level of the PAI-1 promoter in endothelial cells transfected with plasmid p800 LUC containing a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was increased in the presence of ROS, and was suppressed by up to 75 p.c. in the presence of antioxidants. [1]. On the basis of this study we can conclude that reactive oxygen species play an important role in a cytokine-induced activation of PAI-1 expression, and may act as a signal transduction messenger.

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    Tytuł oryginału: Natriuretic peptides reduce plasminogen activator inhibitor-1 expression in human endothelial cells.
    Autorzy: Pawłowska Zofia, Jerczyńska Hanna, Szemraj Janusz, Barańska Patrycja, Świątkowska Maria, Cierniewski Czesław S.
    Źródło: Cell. Mol. Biol. Lett. 2002: 7 (4) s.1153-1157, il., bibliogr. 10 poz.
    Sygnatura GBL: 306,513

    Hasła klasyfikacyjne GBL:
  • farmacja

    Typ dokumentu:
  • praca doświadczalna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: Plasma concentrations of natriuretic peptides increase in some pathological conditions, but very little is known about the effect of these vasodilator peptides on the regulation of the blood coagulation system. The fundamental role in the regulation of fibrinolysis is played by plasminogen activator inhibitor type 1 (PAI-1). Recent studies demonstrate that natriuretic peptides can modulate PAI-1 expression in bovine aortic smooth muscle cells and rat aortic endothelial cells. In this report, we tested the effect of natriuretic peptides on PAI-1 expression in the human endothelial cell line (EA.hy 926). For this purpose, we treated the cell cultures with ANP, BNP and CNP, and modulation of PAI-1 synthesis was evaluated. We compared the effect of natiuretic peptides on synthesis and release of PAI-1 in unstimulated cells, and after activation with tumour necrosis factor ŕ (TNFŕ). Natriuretic peptides abolished TNFŕ- induced upregulation of PAI-1 expression at both the PAI-1 mRNA and the antigen levels. The inhibitory efficiency was higher in the case of CNP when compared to that produced by ANP and BNP, particularly when TNFŕ-stimulated cells were used. We observed an inhibition of stimulatory effect of TNFŕ on PAI-1 expression also at the level of the PAI-1 promoter in cells transfected with a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was markedly inhibited by C-type natriuretic peptide, already at a very low (0.001 ćM) concentration of the peptide.

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