Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: GRUBEK-JAWORSKA
Liczba odnalezionych rekordów: 3



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Tytuł oryginału: Reactivity of alveolar macrophages in lung cancer patients and healthy subjects: surface ICAM-1 after INF-ç stimulation in vitro.
Autorzy: Hoser G., Grubek-Jaworska H., Droszcz P., Domagała-Kulawik J., Dąbrowska M., Chazan R.
Źródło: Folia Histochem. Cytobiol. 2002: 40 (2) s.103-104, il., tab., bibliogr. 6 poz. - Konferencja pt. Cytometria przepływowa i ilościowa, mikroskopia w diagnostyce medycznej Poznań 21-22.05. 2001
Sygnatura GBL: 304,846

Hasła klasyfikacyjne GBL:
  • immunologia
  • farmacja
  • pulmonologia
  • onkologia

    Typ dokumentu:
  • praca związana ze zjazdem
  • praca kazuistyczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie
  • in vitro

    Streszczenie angielskie: The linearity of ICAM-1 expression on alveolar macrophages (AM) before and after INF-ç stimulation in healthy and lung cancaer subjects were compared. AM were collected by bronchoalveolar lavage and incubated with/without INF-ç according to standard procedures. The harvested calls were analyzed by flow cytometry using mooclonal antibodies against leucocytes and macrophages. Only viable cells were analyzed. Stimulation with INF-ç revealed two AM subpopulations of similar size differentiated in the intensity of ICAM-1 expression. They were not distinctly marked in every studied case. our preliminary results did not confirm the previously reported decreasing reactivity of AMs after INF-ç stimulation in lung cancer patients.


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    Tytuł oryginału: Analiza kwasów mikolowych różnych gatunków z rodzaju Mycobacterium metodą wysokociśnieniowej chromatografii cieczowej (HPLC).
    Tytuł angielski: Analysis of mycolic acids from various species Mycobacterium by high pressure liquid chromatography (HPLC).
    Autorzy: Safianowska Aleksandra, Walkiewicz Renata, Grubek-Jaworska Hanna, Zwolska Zofia, Augustynowicz-Kopeć Ewa, Glapiński Jarosław, Chazan Ryszarda
    Źródło: Pneumonol. Alergol. Pol. 2002: 70 (3/4) s.130-138, il., tab., bibliogr. 6 poz., sum.
    Sygnatura GBL: 301,096

    Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Typ dokumentu:
  • praca doświadczalna

    Streszczenie angielskie: HPLC is the most useful method to speciate mycobacteria by analisis of myocolic acids. The purpose was to prepare a library containing chromatographic patterns of mycolic acids derived from reference species Mycobacterium, which had been cultivated in standard conditions. 28 reference strains (27 ones from American Type Culture Collection and one cultivated from the vaccine m. bovis BCG) were used. The analysis of mycolic acids involved chromatographic separation of their bromophenacyl derivatives according to Centers for disease Control recommendation. Mycolic acids profiles formed by HLPC were reproducible for all reference species in this study. Standard deviation of relative retention time of every peak did not exceed 2,5 p.c. The species included into M. tuberculosis complex beyond M. bovis BCG shared the same mycolis acids pattern. HPLC is the only mean to distinguish m. tuberculosis from M. bovis BCG. The other studied stains had species specific patterns which differed from M. tubeculosis complex and M. bovis BCG. The prepared library comprising 28 reference elution profiles of mycolic acids from known mycobacteria species can be applied in diagnostic procedure of tuberculosis and mycobacteriosis.


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    Tytuł oryginału: Zastosowanie analizy kwasów mikolowych w diagnostyce gruźlicy i mikobakterioz - 3 lata doświadczeń.
    Tytuł angielski: Aplication of mycolic acids analysis in diagnostic procedure of tuberculosis and mycobacteriosis - there-year experience.
    Autorzy: Walkiewicz Renata, Safianowska Aleksandra, Grubek-Jaworska Hanna, Zalewska-Sch”nthaler Natalia, Glapiński Jarosław, Chazan Ryszarda
    Źródło: Pneumonol. Alergol. Pol. 2002: 70 (9/10) s.444-449, tab., bibliogr. 16 poz., sum.
    Sygnatura GBL: 301,096

    Hasła klasyfikacyjne GBL:
  • pulmonologia
  • mikrobiologia

    Typ dokumentu:
  • praca kliniczna

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: The aim of the study was to estimate the utility of the HPLC-based method of mycolic acids analysis to classify Mycobacterium species in routine diagnostic procedure on the basis of own three-year experience. 2142 patients' specimens were examined. 141 AFB were cultured. 36.2 p.c. strains were classified as M. tuberculosis complex by HPLC. The idenification was confirmed by AMPLICOR MTB (Roche diagnostic, USA). M. xenopi (17.0 p.c.), M. kansasii (14.2 p.c.) and M. gordonae (14.2 p.c.) were the most frequent identified out of nontuberculous mycobacteria. Four mycobacteriosiss cases were suspected because of repeated identification of the isolated strains. 136 strains on L-J slant shipped from other center were identified. We confirm that the HPLC method is highly effective and specific for Mycobacterium species classification, which can be performed in no more than a couple of hour. In opinion it is a very helpful tool, hard to replace in diagnostic procedure of tuberculosis and mycobacteriosis.

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