Wynik wyszukiwania w bazie Polska Bibliografia Lekarska GBL

Zapytanie: BINDUGA-GAJEWSKA
Liczba odnalezionych rekordów: 3



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Tytuł oryginału: Evaluation of the usefulness of the ELISA method for detection of enterovirus antibodies in serum samples of patients with myocarditis.
Autorzy: Wielkopolska Anna, Gut Włodzimierz, Binduga-Gajewska Iwona, Jarząbek Zdzisław
Źródło: Med. Sci. Monitor 2002: 8 (1) s.MT10-MT14, il., bibliogr. 26 poz.
Sygnatura GBL: 313,278

Hasła klasyfikacyjne GBL:
  • immunologia
  • mikrobiologia
  • kardiologia

    Typ dokumentu:
  • praca kliniczna
  • tytuł obcojęzyczny

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Background: Enteroviruses are among the etiological factors in myocarditis, In the majority of cases it is impossible to obtain a direct confirmation of the presence of the virus, and typologically specific methods are of limited applicability, due both to the number of enteroviruses that can cause myocarditits, and to the serological cross reactions among them. The goal of our research was to determine the usability of the ELISA test and the complement fixation reaction (CFR) in diagnostic testing of the serum of patients with myocarditis. Material/Methods: The research material consisted of 1197 serum samples collected from patients with myocarditis. In all these samples we measured the contentration of IgG adn IgM enterovirus antibodies using the ELISA method, and teh height of the titer using the CFR method. Results: The presence of IgM antibodies is a confirmation of current infection. A postitive result was obtained for 61 p.c. of the patients. On the other hand, if one accepts as confirmation of infection a titer significantly different from the level observed in thy healthy population, namely ň 16 for CFR and ň 11 for ELISA-IgG (cf. positive results of 33 p.c. for CFR and 18.8 p.c. for ELISA IgG in our patients), the results may be disproportional. If one assumes as a test criterion a significant increase in titer, the presence of infection was confirmed in 28.5 p.c. of cases for CFR and 62 p.c. for ELISA-IgG. Conclusions: An analysis of the concentration of IgG antibodies constitutes a clear confirmation of infection., if the criterion of evaluation is based on a measure in a single serum sample. In this case it is necessary to test for a significant increase in the titer. The use of the average titer for the population at large to evaluate results leads to both false positives and false negatives. Testing for IgM antibodies in a single serum sample is a faster method which confirms the current presence of infection. Our results indicate that the ELISA test can be used to diagnose myocarditis, since


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    Tytuł oryginału: Ocena przydatności molekularnych metod wykrycia genomu enterowirusów w różnicowej diagnostyce wirusowych neuroinfekcji na tle ogólnych problemów diagnostyki zakażeń enterowirusowych w Polsce : praca doktorska
    Autorzy: Binduga-Gajewska Iwona, Gut Włodzimierz (promot.).; Państwowy Zakład Higieny Zakład Wirusologii w Warszawie
    Źródło: 2002, [10], 123 k. : il., tab., bibliogr. 110 poz., streszcz., maszyn.
    Sygnatura GBL: 45/20082

    Hasła klasyfikacyjne GBL:
  • pediatria
  • genetyka
  • mikrobiologia
  • neurologia
  • choroby zakaźne

    Typ dokumentu:
  • praca doktorska
  • praca epidemiologiczna

    Wskaźnik treści:
  • ludzie
  • dzieci 2-5 r.ż.
  • dzieci 6-12 r.ż.
  • dzieci 13-18 r.ż.
  • płeć męska
  • płeć żeńska


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    Tytuł oryginału: Diagnostyka zakażeń enterowirusami niepoliomyelitycznymi - aktualne kierunki i problemy.
    Tytuł angielski: Diagnosis of nonpolio enterovirus infections - current trends and problems.
    Autorzy: Binduga-Gajewska Iwona, Gut Włodzimierz
    Źródło: Post. Mikrobiol. 2002: 41 (4) s.367-381, il., bibliogr. 76 poz., sum.
    Sygnatura GBL: 304,786

    Hasła klasyfikacyjne GBL:
  • mikrobiologia

    Wskaźnik treści:
  • ludzie

    Streszczenie angielskie: Enteroviruses are the most commonly identified caused of viral meningitis. In temporary climate infection caused by this group of viruses have seasonal distribution with a domination of certain entertovirus serotypes. Isolation of the virus in cell culture is taken as the gold standard in the diagnosis of diseases caused by enterovirus, despite of its lack of usefulness in rapid diagnosis. Demonstration anti-enterovirus IgM in a single serum sample allows a more rapid diagnosis but the results obtained by this means has to be interpreting with caution, especially in epidemiological seasons. Recent developments in nucleic acid amplification techniques now allow the rapid and sensitive diagnosis of enterovirus infections. When diagnosing the virus in cerebrospinal fluid, isolation of the virus has been largely replaced by the rapid demonstration of the virus using RT-PCR.

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